Monoclonal Antibodies

Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of TAF7 over-expressed 293 cell line, cotransfected with TAF7 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TAF7 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged TAF7 is ~0.3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TAF7 on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TAF7 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 1ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TAF7 on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of TAF7 expression in transfected 293T cell line by TAF7 monoclonal antibody. Lane 1: TAF7 transfected lysate (40.3kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (TAF7 monoclonal antibody, Western Blot analysis of TAF7 expression in MCF-7.)

Compare (5 max)

TAF7, Monoclonal Antibody (Cat# AAA25271)

Full Name

TAF7 (TAF2F, TAFII55, Transcription Initiation Factor TFIID Subunit 7, RNA Polymerase II TBP-associated Factor Subunit F, Transcription Initiation Factor TFIID 55kD Subunit, TAF(II)55, TAFII-55) (FITC)

Gene Names

TAF7; TAF2F; TAFII55

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

Application Data (Detection limit for recombinant GST tagged HDAC1 is approximately 1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HDAC1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 0.7 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HDAC1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 0.7 ug/ml])

WB (Western Blot) (HDAC1 monoclonal antibody (M11), clone 5A11 Western Blot analysis of HDAC1 expression in Hela S3 NE.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HDAC1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HDAC1 on HeLa cell. [antibody concentration 10 ug/ml])

Compare (5 max)

HDAC1, Monoclonal Antibody (Cat# AAA26054)

Full Name

HDAC1 (Histone Deacetylase 1, DKFZp686H12203, GON-10, HD1, RPD3, RPD3L1) (APC)

Gene Names

HDAC1; HD1; RPD3; KDAC1; GON-10; RPD3L1

Applications

IF, IHC, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SNAI2 on HepG2 cell. [antibody concentration 10ug/ml].)

WB (Western Blot) (SNAI2 monoclonal antibody (M05). Western Blot analysis of SNAI2 expression in NIH/3T3.)

WB (Western Blot) (SNAI2 monoclonal antibody (M05). Western Blot analysis of SNAI2 expression in Raw 264.7.)

WB (Western Blot) (SNAI2 monoclonal antibody (M05), Western Blot analysis of SNAI2 expression in HepG2.)

WB (Western Blot) (SNAI2 monoclonal antibody. Western Blot analysis of SNAI2 expression in PC-12.)

WB (Western Blot) (SNAI2 monoclonal antibody. Western Blot analysis of SNAI2 expression in human liver.)

WB (Western Blot) (Western Blot detection against Immunogen (34.14kD).)

Compare (5 max)

SNAI2, Monoclonal Antibody (Cat# AAA25550)

Full Name

SNAI2 (Zinc Finger Protein SNAI2, Neural Crest Transcription Factor Slug, Protein Snail Homolog 2, SLUG, SLUGH, MGC10182) (HRP)

Gene Names

SNAI2; SLUG; WS2D; SLUGH; SLUGH1; SNAIL2

Reactivity

Human, Mouse, Rat

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (ZNF622 monoclonal antibody. Western Blot analysis of ZNF622 expression in Raw 264.7.)

WB (Western Blot) (ZNF622 monoclonal antibody. Western Blot analysis of ZNF622 expression in Hela NE.)

Application Data (Detection limit for recombinant GST tagged ZNF622 is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ZNF622 on HeLa cell. [antibody concentration 10ug/ml].)

WB (Western Blot) (Western Blot analysis of ZNF622 expression in transfected 293T cell line by ZNF622 monoclonal antibody. Lane 1: ZNF622 transfected lysate (54.3kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (ZNF622 monoclonal antibody. Western Blot analysis of ZNF622 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)

Compare (5 max)

ZNF622, Monoclonal Antibody (Cat# AAA25296)

Full Name

ZNF622 (Zinc Finger Protein 622, Zinc Finger-like Protein 9, ZPR9) (FITC)

Reactivity

Human, Mouse, Rat

Applications

EIA, IF, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

Application Data (Detection limit for recombinant GST tagged GBA is ~1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GBA on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GBA on formalin-fixed paraffin-embedded human breast cancer. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of GBA expression in transfected 293T cell line by GBA monoclonal antibody. Lane 1: GBA transfected lysate (60kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (GBA monoclonal antibody, Western Blot analysis of GBA expression in MCF-7.)

WB (Western Blot) (Western Blot detection against Immunogen (35.64kD).)

Compare (5 max)

GBA, Monoclonal Antibody (Cat# AAA24813)

Full Name

GBA (Glucosylceramidase, Acid beta-glucosidase, Alglucerase, Beta-glucocerebrosidase, D-glucosyl-N-acylsphingosine Glucohydrolase, Imiglucerase, GC, GLUC) (Biotin)

Gene Names

GBA; GCB; GBA1; GLUC

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western Blot detection against Immunogen (37kD).)

Application Data (Detection limit for recombinant GST tagged GMNN is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GMNN on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GMNN on formalin-fixed paraffin-embedded human placenta. [antibody concentration 1ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GMNN on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of GMNN expression in transfected 293T cell line by GMNN monoclonal antibody. Lane 1: GMNN transfected lysate (23.6kD). Lane 2: Non-transfected lysate.)

Compare (5 max)

Geminin, Monoclonal Antibody (Cat# AAA24815)

Full Name

Geminin (GMNN, GEM, Geminin DNA Replication Inhibitor, DNA Replication Inhibitor, RP3-369A17.3) (Biotin)

Gene Names

GMNN; Gem; MGORS6

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (MARCH7 monoclonal antibody. Western Blot analysis of 'MARCH7 expression in PC-12.)

Application Data (Detection limit for recombinant GST tagged MARCH7 is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MARCH7 on HeLa cell. [antibody concentration 10ug/ml].)

WB (Western Blot) (MARCH7 monoclonal antibody, Western Blot analysis of MARCH7expression in K-562.)

WB (Western Blot) (MARCH7 monoclonal antibody. Western Blot analysis of 'MARCH7 expression in Raw 264.7.)

WB (Western Blot) (Western Blot detection against Immunogen (33.92kD).)

Compare (5 max)

2019-03-07, Monoclonal Antibody (Cat# AAA24562)

Full Name

MARCH7 (E3 Ubiquitin-protein Ligase MARCH7, Axotrophin, Membrane-associated RING Finger Protein 7, Membrane-associated RING-CH Protein VII, MARCH-VII, RING Finger Protein 177, AXOT, RNF177, DKFZp586F1122) APC

Gene Names

MARCH7; AXO; AXOT; RNF177; MARCH-VII

Reactivity

Human, Mouse, Rat

Applications

EIA, IF, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

Application Data (Detection limit for recombinant GST tagged SNRPA is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SNRPA on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SNRPA on formalin-fixed paraffin-embedded human heart tissue. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of SNRPA expression in transfected 293T cell line by SNRPA monoclonal antibody.Lane 1: SNRPA transfected lysate (31.3kD).Lane 2: Non-transfected lysate.)

WB (Western Blot) (SNRPA monoclonal antibody, Western Blot analysis of SNRPA expression in Hela.)

WB (Western Blot) (Western Blot detection against Immunogen (57.13kD).)

Compare (5 max)

SNRPA, Monoclonal Antibody (Cat# AAA14771)

Full Name

SNRPA (U1 Small Nuclear Ribonucleoprotein A, U1 snRNP A, U1-A, U1A)

Gene Names

snrpa; u1a; mud1

Reactivity

Human

Applications

EL/EIA, WB, IHC, IF

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

$820/0.1 mg $1,565/2x0.1 mg $2,270/3x0.1 mg $2,940/4x0.1 mg $3,380/5x0.1 mg

< >

<< Previous Next >>

WB (Western Blot) (LIMK1 monoclonal antibody. Western Blot analysis of LIMK1 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

Application Data (Detection limit for recombinant GST tagged LIMK1 is ~0.03ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to LIMK1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3ug/ml])

WB (Western Blot) (LIMK1 monoclonal antibody. Western Blot analysis of LIMK1 expression in NIH/3T3.)

WB (Western Blot) (LIMK1 monoclonal antibody. Western Blot analysis of LIMK1 expression in Raw 264.7.)

WB (Western Blot) (LIMK1 monoclonal antibody Western Blot analysis of LIMK1 expression in SW-13.)

Compare (5 max)

LIMK1, Monoclonal Antibody (Cat# AAA24259)

Full Name

LIMK1 (LIM Domain Kinase 1, LIMK-1, LIMK) (AP)

Gene Names

LIMK1; LIMK; LIMK-1

Reactivity

Human, Mouse, Rat

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

Application Data (Detection limit for recombinant GST tagged SP1 is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SP1 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 1ug/ml].)

WB (Western Blot) (SP1 monoclonal antibody. Western Blot analysis of SP1 expression in NIH/3T3.)

WB (Western Blot) (SP1 monoclonal antibody, Western Blot analysis of SP1 expression in Hela NE.)

WB (Western Blot) (Western Blot detection against Immunogen (38.21kD).)

Compare (5 max)

SP1, Monoclonal Antibody (Cat# AAA25555)

Full Name

SP1 (Transcription Factor Sp1, TSFP1) (HRP)

Reactivity

Human, Mouse

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

Application Data (Staining of NALM6 cells with MOUSE ANTI HUMAN CD10:ALEXA 488)

Application Data (Staining of human peripheral blood granulocytes with MOUSE ANTI HUMAN CD10:RPE)

Application Data (Staining of human peripheral blood granulocytes with MOUSE ANTI HUMAN CD10:FITC)

Application Data (Staining of human peripheral blood granulocytes with MOUSE ANTI HUMAN CD10:BIOTIN)

Application Data (Staining of human peripheral blood granulocytes with MOUSE ANTI HUMAN CD10:AZIDE FREE)

Application Data (Staining of human peripheral blood granulocytes with MOUSE ANTI HUMAN CD10:APC)

Compare (5 max)

CD10, Monoclonal Antibody (Cat# AAA26831)

Full Name

CD10 (CALLA, CD 10, Common Acute Lymphocytic Leukemia Antigen, Enkephalinase, gp100, Membrane Metalloendopeptidase, MME, Neprilysin, Neutral Endopeptidase, Pmel17) (MaxLight 750)

Gene Names

MME; NEP; SFE; CD10; CALLA

Reactivity

Human

Applications

FC/FACS, IHC, IP, WB

Purity

Purified by protein G affinity chromatography from tissue culture supernatant.

Pricing

$950/0.1 mL $1,830/2x0.1 mL $2,660/3x0.1 mL $3,440/4x0.1 mL $3,985/5x0.1 mL

< >

<< Previous Next >>

Application Data (Detection limit for recombinant GST tagged NKX3-1 is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NKX3-1 on NIH/3T3 cell. [antibody concentration 10ug/ml])

WB (Western Blot) (NKX3-1 monoclonal antibody. Western Blot analysis of NKX3-1 expression in NIH/3T3.)

WB (Western Blot) (NKX3-1 monoclonal antibody. Western Blot analysis of NKX3-1 expression in Raw 264.7.)

WB (Western Blot) (NKX3-1 monoclonal antibody. Western Blot analysis of NKX3-1 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)

Compare (5 max)

NKX3-1, Monoclonal Antibody (Cat# AAA24290)

Full Name

NKX3-1 (Homeobox Protein Nkx-3.1, Homeobox Protein NK-3 Homolog A, NKX3.1, NKX3A) (AP)

Gene Names

NKX3-1; NKX3; BAPX2; NKX3A; NKX3.1

Reactivity

Human, Mouse, Rat

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

Application Data (Detection limit for recombinant GST tagged UBTF is approximately 1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to UBTF on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to UBTF on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])

WB (Western Blot) (UBTF monoclonal antibody (M04), clone 2D8 Western Blot analysis of UBTF expression in HepG2.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to UBTF on HepG2 cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to UBTF on HepG2 cell. [antibody concentration 10 ug/ml])

Compare (5 max)

UBTF, Monoclonal Antibody (Cat# AAA26104)

Full Name

UBTF (Upstream Binding Transcription Factor, RNA Polymerase I, NOR-90, UBF) (APC)

Gene Names

UBTF; UBF; UBF1; UBF2; UBF-1; CONDBA; NOR-90

Applications

IF, IHC, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Figure 7. IHC analysis of Poliovirus Receptor/PVR using anti-Poliovirus Receptor/PVR antibody (AAA19668).Poliovirus Receptor/PVR was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Poliovirus Receptor/PVR Antibody (AAA19668) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of Poliovirus Receptor/PVR using anti-Poliovirus Receptor/PVR antibody (AAA19668).Poliovirus Receptor/PVR was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Poliovirus Receptor/PVR Antibody (AAA19668) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of Poliovirus Receptor/PVR using anti-Poliovirus Receptor/PVR antibody (AAA19668).Poliovirus Receptor/PVR was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Poliovirus Receptor/PVR Antibody (AAA19668) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of Poliovirus Receptor/PVR using anti-Poliovirus Receptor/PVR antibody (AAA19668).Poliovirus Receptor/PVR was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Poliovirus Receptor/PVR Antibody (AAA19668) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of Poliovirus Receptor/PVR using anti-Poliovirus Receptor/PVR antibody (AAA19668).Poliovirus Receptor/PVR was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Poliovirus Receptor/PVR Antibody (AAA19668) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of Poliovirus Receptor/PVR using anti-Poliovirus Receptor/PVR antibody (AAA19668).Poliovirus Receptor/PVR was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Poliovirus Receptor/PVR Antibody (AAA19668) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of Poliovirus Receptor/PVR using anti-Poliovirus Receptor/PVR antibody (AAA19668).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human A431 whole cell lysates,Lane 2: human A549 whole cell lysates,Lane 3: human HT1080 whole cell lysates,Lane 4: human Daudi whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Poliovirus Receptor/PVR antigen affinity purified monoclonal antibody (#AAA19668) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Poliovirus Receptor/PVR at approximately 70-80 kDa. The expected band size for Poliovirus Receptor/PVR is at 45 kDa.)

Compare (5 max)

Receptor/PVR, Monoclonal Antibody (Cat# AAA19668)

Full Name

Anti-Poliovirus Receptor/PVR Antibody Picoband (monoclonal, 9B9F1)

Gene Names

PVR; PVS; HVED; CD155; NECL5; TAGE4; Necl-5

Reactivity

Human, Mouse

Applications

WB, IHC

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

< >

<< Previous Next >>

WB (Western Blot) (AKT3 monoclonal antibody (M03), clone 3F3. Western Blot analysis of AKT3 expression in PC-12 (Cat # L012V1).)

WB (Western Blot) (AKT3 monoclonal antibody (M03), clone 3F3. Western Blot analysis of AKT3 expression in Raw 264.7 (Cat # L024V1).)

WB (Western Blot) (AKT3 monoclonal antibody (M03), clone 3F3. Western Blot analysis of AKT3 expression in NIH/3T3 (Cat # L018V1).)

Application Data (Detection limit for recombinant GST tagged AKT3 is approximately 0.03ng/ml as a capture antibody.)

WB (Western Blot) (AKT3 monoclonal antibody (M03), clone 3F3. Western Blot analysis of AKT3 expression in MCF-7 (Cat # L046V1).)

WB (Western Blot) (AKT3 monoclonal antibody (M03), clone 3F3 Western Blot analysis of AKT3 expression in HeLa (Cat # L013V1).)

Compare (5 max)

AKT3, Monoclonal Antibody (Cat# AAA26312)

Full Name

AKT3 (v-akt murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, gamma), DKFZp434N0250, PKB-GAMMA, PKBG, PRKBG, RAC-PK-gamma, RAC-gamma, STK-2) (FITC)

Gene Names

AKT3; MPPH; PKBG; MPPH2; PRKBG; STK-2; PKB-GAMMA; RAC-gamma; RAC-PK-gamma

Applications

WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (NDN monoclonal antibody Western Blot analysis of NDN expression in HL-60.)

WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)

Compare (5 max)

NDN, Monoclonal Antibody (Cat# AAA24283)

Full Name

NDN (Necdin) (AP)

Gene Names

NDN; PWCR; HsT16328

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (AKT2 monoclonal antibody (M03), clone 1D9. Western Blot analysis of AKT2 expression in PC-12.)

WB (Western Blot) (AKT2 monoclonal antibody (M03), clone 1D9. Western Blot analysis of AKT2 expression in NIH/3T3.)

Application Data (Detection limit for recombinant GST tagged AKT2 is approximately 1ng/ml as a capture antibody.)

WB (Western Blot) (AKT2 monoclonal antibody (M03), clone 1D9 Western Blot analysis of AKT2 expression in Jurkat.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to AKT2 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to AKT2 on HeLa cell. [antibody concentration 10 ug/ml])

Compare (5 max)

AKT2, Monoclonal Antibody (Cat# AAA26098)

Full Name

AKT2 (v-akt Murine Thymoma Viral Oncogene Homolog 2, PKBB, PKBBETA, PRKBB, RAC-BETA) (APC)

Gene Names

AKT2; PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA

Applications

IF, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of THP-1 cells using anti-INPPL1 antibody (AAA19364).Overlay histogram showing THP-1 cells stained with AAA19364 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- INPPL1 Antibody (AAA19364, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 7. IF analysis of INPPL1 using anti- INPPL1 antibody (AAA19364).INPPL1 was detected in immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti- INPPL1 Antibody (AAA19364) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of INPPL1 using anti-INPPL1 antibody (AAA19364).INPPL1 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-INPPL1 Antibody (AAA19364) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of INPPL1 using anti-INPPL1 antibody (AAA19364).INPPL1 was detected in paraffin-embedded section of human renal clear cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-INPPL1 Antibody (AAA19364) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of INPPL1 using anti-INPPL1 antibody (AAA19364).INPPL1 was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-INPPL1 Antibody (AAA19364) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of INPPL1 using anti-INPPL1 antibody (AAA19364).INPPL1 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-INPPL1 Antibody (AAA19364) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of INPPL1 using anti-INPPL1 antibody (AAA19364).INPPL1 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-INPPL1 Antibody (AAA19364) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of INPPL1 using anti-INPPL1 antibody (AAA19364).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human Hela whole cell lysatesLane 2: human Raji whole cell lysatesLane 3: human PC-3 whole cell lysatesLane 4: rat C6 whole cell lysatesLane 5: mouse NIH/3T3 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- INPPL1 antigen affinity purified monoclonal antibody (Catalog # AAA19364) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for INPPL1 at approximately 150KD. The expected band size for INPPL1 is at 150KD.)

Compare (5 max)

INPPL1, Monoclonal Antibody (Cat# AAA19364)

Full Name

Anti-INPPL1 Antibody (monoclonal, 8C13)

Gene Names

INPPL1; OPSMD; SHIP2

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, ICC, IF, FC/FACS/FCM

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to UBTF on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.5 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to UBTF on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.5 ug/ml])

Application Data (Detection limit for recombinant GST tagged UBTF is approximately 1ng/ml as a capture antibody.)

WB (Western Blot) (UBTF monoclonal antibody (M03), clone 1A2 Western Blot analysis of UBTF expression in HepG2 (Cat # L019V1).)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to UBTF on HepG2 cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to UBTF on HepG2 cell. [antibody concentration 10 ug/ml])

Compare (5 max)

UBTF, Monoclonal Antibody (Cat# AAA26363)

Full Name

UBTF (Upstream Binding Transcription Factor, RNA Polymerase I, NOR-90, UBF) (FITC)

Gene Names

UBTF; UBF; UBF1; UBF2; UBF-1; CONDBA; NOR-90

Applications

IF, IHC, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

Application Data (Proximity Ligation Analysis of protein-protein interactions between TP53 and PML HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-PML mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)

Application Data (Detection limit for recombinant GST tagged PML is ~0.3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PML on HeLa cell. [antibody concentration 10ug/ml])

WB (Western Blot) (PML monoclonal antibody Western Blot analysis of PML expression in Hela NE.)

WB (Western Blot) (PML monoclonal antibody Western Blot analysis of PML expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (36.63kD).)

Compare (5 max)

PML, Monoclonal Antibody (Cat# AAA25505)

Full Name

PML (Probable Transcription Factor PML, RING Finger Protein 71, Tripartite Motif-containing Protein 19, PML, MYL, RNF71, TRIM19) (HRP)

Gene Names

PML; MYL; RNF71; PP8675; TRIM19

Reactivity

Human, Rat

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western Blot analysis of OPA1 expression in transfected 293T cell line by OPA1 monoclonal antibody (M06), clone 8A32.Lane 1: OPA1 transfected lysate (Predicted MW: 111.6 KDa).Lane 2: Non-transfected lysate.)

Application Data (Detection limit for recombinant GST tagged OPA1 is 0.1 ng/ml as a capture antibody.)

WB (Western Blot) (OPA1 monoclonal antibody (M06), clone 8A32. Western Blot analysis of OPA1 expression in rat brain.)

WB (Western Blot) (OPA1 monoclonal antibody (M06), clone 8A32. Western Blot analysis of OPA1 expression in human ovarian cancer.)

WB (Western Blot) (OPA1 monoclonal antibody (M06), clone 8A32. Western Blot analysis of OPA1 expression in PC-12.)

WB (Western Blot) (OPA1 monoclonal antibody (M06), clone 8A32. Western Blot analysis of OPA1 expression in Daoy.)

Compare (5 max)

OPA1, Monoclonal Antibody (Cat# AAA26020)

Full Name

OPA1 (Pptic Atrophy 1 (Autosomal Dominant), FLJ12460, KIAA0567, MGM1, NPG, NTG, LargeG) (AP)

Gene Names

OPA1; NPG; NTG; MGM1; BERHS; largeG; MTDPS14

Applications

EIA, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (CA1 monoclonal antibody, Western Blot analysis of CA1 expression in HeLa.)

Application Data (Detection limit for recombinant GST tagged CA1 is ~0.03ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of CA1 transfected lysate using 124177 and Protein A Magnetic Bead and immunoblotted with CA1 rabbit polyclonal antibody.)

WB (Western Blot) (Western Blot analysis of CA1 expression in transfected 293T cell line using 124177. Lane 1: CA1 transfected lysate (28.9kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot analysis of CA1 expression in human spleen using 124177)

WB (Western Blot) (Western Blot detection against Immunogen (54.45kD), using 124177.)

Compare (5 max)

CA1, Monoclonal Antibody (Cat# AAA25039)

Full Name

CA1 (Carbonic Anhydrase 1, Carbonic Anhydrase I, CA-I, Carbonate Dehydratase I, Carbonic Anhydrase B, CAB) (FITC)

Gene Names

CA1; CAB; CA-I; Car1; HEL-S-11

Reactivity

Human

Applications

EIA, IP, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (BATF monoclonal antibody Western Blot analysis of BATF expression in Hela NE.)

WB (Western Blot) (BATF monoclonal antibody Western Blot analysis of BATF expression in PC-12.)

Application Data (Detection limit for recombinant GST tagged BATF is ~1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of BATF transfected lysate using BATF monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with BATF rabbit polyclonal antibody.)

WB (Western Blot) (Western Blot analysis of BATF expression in transfected 293T cell line by BATF monoclonal antibody Lane 1: BATF transfected lysate (14.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (BATF monoclonal antibody Western Blot analysis of BATF expression in NIH/3T3.)

WB (Western Blot) (Western Blot detection against Immunogen (36.23kD).)

Compare (5 max)

BATF, Monoclonal Antibody (Cat# AAA25034)

Full Name

BATF (Basic Leucine Zipper Transcriptional Factor ATF-like, B Cell-activating Transcription Factor, SF-HT-activated Gene 2 Protein) (FITC)

Gene Names

BATF; SFA2; B-ATF; BATF1; SFA-2

Reactivity

Human, Mouse, Rat

Applications

EIA, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (ACTN4 monoclonal antibody Western Blot analysis of ACTN4 expression in HeLa NE.)

WB (Western Blot) (ACTN4 monoclonal antibody Western Blot analysis of ACTN4 expression in NIH/3T3.)

Application Data (Detection limit for recombinant GST tagged ACTN4 is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ACTN4 on HeLa cell. [antibody concentration 20ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ACTN4 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of ACTN4 expression in transfected 293T cell line by ACTN4 monoclonal antibody. Lane 1: ACTN4 transfected lysate (104.9kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (ACTN4 monoclonal antibody Western Blot analysis of ACTN4 expression in PC-12.)

Compare (5 max)

ACTN4, Monoclonal Antibody (Cat# AAA25305)

Full Name

ACTN4 (Alpha-actinin-4, Non-muscle alpha-actinin 4, F-actin Cross-linking Protein) (HRP)

Gene Names

ACTN4; FSGS; FSGS1; ACTININ-4

Reactivity

Human, Mouse, Rat

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 11. Flow Cytometry analysis of RH35 cells using anti- eRF1/ETF1 antibody (AAA19382).Overlay histogram showing RH35 cells stained with AAA19382 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-eRF1/ETF1 Antibody (AAA19382, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of HEPA1-6 cells using anti- eRF1/ETF1 antibody (AAA19382).Overlay histogram showing HEPA1-6 cells stained with AAA19382 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-eRF1/ETF1 Antibody (AAA19382, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of CACO-2 cells using anti- eRF1/ETF1 antibody (AAA19382).Overlay histogram showing CACO-2 cells stained with AAA19382 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-eRF1/ETF1 Antibody (AAA19382, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 8. IF analysis of eRF1/ETF1 using anti- eRF1/ETF1 antibody (AAA19382).eRF1/ETF1 was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti- eRF1/ETF1 Antibody (AAA19382) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of eRF1/ETF1 using anti-eRF1/ETF1 antibody (AAA19382).eRF1/ETF1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-eRF1/ETF1 Antibody (AAA19382) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of eRF1/ETF1 using anti-eRF1/ETF1 antibody (AAA19382).eRF1/ETF1 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-eRF1/ETF1 Antibody (AAA19382) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of eRF1/ETF1 using anti-eRF1/ETF1 antibody (AAA19382).eRF1/ETF1 was detected in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-eRF1/ETF1 Antibody (AAA19382) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of eRF1/ETF1 using anti-eRF1/ETF1 antibody (AAA19382).eRF1/ETF1 was detected in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-eRF1/ETF1 Antibody (AAA19382) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of eRF1/ETF1 using anti-eRF1/ETF1 antibody (AAA19382).eRF1/ETF1 was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-eRF1/ETF1 Antibody (AAA19382) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of eRF1/ETF1 using anti-eRF1/ETF1 antibody (AAA19382).eRF1/ETF1 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-eRF1/ETF1 Antibody (AAA19382) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of eRF1/ETF1 using anti-eRF1/ETF1 antibody (AAA19382).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hela whole cell lysatesLane 2: human Jurkat whole cell lysatesLane 3: human K562 whole cell lysatesLane 4: human Raji whole cell lysatesLane 5: human HEPG2 whole cell lysatesLane 6: rat PC-12 whole cell lysatesLane 7: mouse RAW264. 7 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti-eRF1/ETF1 antigen affinity purified monoclonal antibody (Catalog # AAA19382) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for eRF1/ETF1 at approximately 49KD. The expected band size for eRF1/ETF1 is at 49KD.)

Compare (5 max)

eRF1/ETF1, Monoclonal Antibody (Cat# AAA19382)

Full Name

Anti-eRF1/ETF1 Antibody (monoclonal, 3B6)

Gene Names

ETF1; ERF; RF1; ERF1; TB3-1; D5S1995; SUP45L1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, ICC, IF, FC/FACS/FCM

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Tissue: hepatocytes. Species: Rat. Fixation: Paraffin Embedded. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:200. Liver sections were paraffin embedded. First pictures in series show two hours after exposure to stress, the second shows the control. Courtesy of: G. Matic, University of Belgrade, Serbia.)

WB (Western Blot) (Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000. Secondary Antibody: HRP Goat Anti-Rat.)

ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Tissue: hepatocyte nuclei. Species: Rat. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:200. Liver sections were paraffin embedded. First pictures in series show two hours after exposure to stress, the second shows the control. Courtesy of: G. Matic, University of Belgrade, Serbia.)

WB (Western Blot) (Western Blot analysis of Bovine MDBK cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. HSP70/HSC70 cells stained brown.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. Inflammatory cells. HSP70/HSC70 stained brown.)

Compare (5 max)

Hsp70/Hsc70, Monoclonal Antibody (Cat# AAA17795)

Full Name

Hsp70/Hsc70 Antibody: RPE

Gene Names

HSPA2; HSP70

Reactivity

Human, Mouse, Rat, Sheep, Dog, Beluga, Bovine, Fish, Guinea pig, Scallop pig, Hamster, Rabbit, Chicken, Xenopus, Drosophila, Yeast

Applications

WB, IP, IHC

Pricing

$675/0.2 mg $1,290/2x0.2 mg $1,875/3x0.2 mg $2,420/4x0.2 mg $2,650/5x0.2 mg

< >

<< Previous Next >>

WB (Western Blot) (NEK9 monoclonal antibody. Western Blot analysis of NEK9 expression in PC-12.)

WB (Western Blot) (NEK9 monoclonal antibody Western Blot analysis of NEK9 expression in HeLa.)

Application Data (Detection limit for recombinant GST tagged NEK9 is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NEK9 on HeLa cell. [antibody concentration 10ug/ml].)

WB (Western Blot) (Western Blot analysis of NEK9 expression in transfected 293T cell line by NEK9 monoclonal antibody. Lane 1: NEK9 transfected lysate (107.2kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (NEK9 monoclonal antibody. Western Blot analysis of NEK9 expression in NIH/3T3.)

WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)

Compare (5 max)

NEK9, Monoclonal Antibody (Cat# AAA25767)

Full Name

NEK9 (Serine/Threonine-protein Kinase Nek9, Nercc1 Kinase, Never in Mitosis A-related Kinase 9, NimA-related Protein Kinase 9, NimA-related Kinase 8, Nek8, KIAA1995, NEK8, NERCC, DKFZp434D0935, MGC138306, MGC16714, NERCC1) (PE)

Gene Names

NEK9; NC; APUG; NERCC; LCCS10; NERCC1

Reactivity

Human, Mouse, Rat

Applications

EIA, IF, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

IP (Immunoprecipitation) (Immunoprecipitation of USP33 transfected lysate using anti-USP33 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with USP33 rabbit polyclonal antibody.)

Application Data (Detection limit for recombinant GST tagged USP33 is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to USP33 on HeLa cell. [antibody concentration 20ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to USP33 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of USP33 expression in transfected 293T cell line by USP33 monoclonal antibody Lane 1: USP33 transfected lysate (103.3kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)

Compare (5 max)

USP33, Monoclonal Antibody (Cat# AAA24996)

Full Name

USP33 (Ubiquitin-specific-processing Protease 33, Ubiquitin Carboxyl-terminal Hydrolase 33, Deubiquitinating Enzyme 33, Ubiquitin Thioesterase 33, VHL-interacting Deubiquitinating Enzyme 1, VDU1, hVDU1, KIAA1097) (Biotin)

Gene Names

USP33; VDU1

Reactivity

Human

Applications

EIA, IF, IHC, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

FCM (Flow Cytometry) (Flow cytometric analysis of MCF-7 cells with Cdk4 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining Cdk4 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Cdk4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Cdk4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human gastric carcinoma tissue using anti-Cdk4 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cdk4 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Cdk4 on Hela cells lysates using anti-Cdk4 antibody at 1/1, 000 dilution.)

Compare (5 max)

CDK4, Monoclonal Antibody (Cat# AAA30186)

Full Name

CDK4 Antibody

Gene Names

CDK4; CMM3; PSK-J3

Reactivity

Human

Applications

WB, ICC, IF, IHC, FC/FACS

Purity

ProA affinity purified

Pricing

$380/0.05 mL $525/0.1 mL $995/2x0.1 mL $1,440/3x0.1 mL $1,865/4x0.1 mL $2,070/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NFKB1 on HeLa cell. [antibody concentration 10 ug/ml])

Application Data (Proximity Ligation Analysis of protein-protein interactions between HSP90AB1 and NFKB1 HeLa cells were stained with anti-HSP90AB1 rabbit purified polyclonal 1:1200 and anti-NFKB1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)

Application Data (Detection limit for recombinant GST tagged NFKB1 is approximately 3ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NFKB1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1.5 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NFKB1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1.5 ug/ml])

WB (Western Blot) (Western Blot analysis of NFKB1 expression in transfected 293T cell line by NFKB1 monoclonal antibody (M02), clone 4A11.Lane 1: NFKB1 transfected lysate(105.4 KDa).Lane 2: Non-transfected lysate.)

Compare (5 max)

NFKB1, Monoclonal Antibody (Cat# AAA26094)

Full Name

NFKB1 (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1, DKFZp686C01211, EBP-1, KBF1, MGC54151, NF-kappa-B, NFKB-p105, NFKB-p50, p105, p50) (APC)

Gene Names

NFKB1; p50; KBF1; p105; EBP-1; NF-kB; CVID12; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B1

Applications

IF, IHC, WB, Cell

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (BATF monoclonal antibody Western Blot analysis of BATF expression in Hela NE.)

WB (Western Blot) (BATF monoclonal antibody Western Blot analysis of BATF expression in PC-12.)

Application Data (Detection limit for recombinant GST tagged BATF is ~1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of BATF transfected lysate using BATF monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with BATF rabbit polyclonal antibody.)

WB (Western Blot) (Western Blot analysis of BATF expression in transfected 293T cell line by BATF monoclonal antibody Lane 1: BATF transfected lysate (14.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (BATF monoclonal antibody Western Blot analysis of BATF expression in NIH/3T3.)

WB (Western Blot) (Western Blot detection against Immunogen (36.23kD).)

Compare (5 max)

BATF, Monoclonal Antibody (Cat# AAA25330)

Full Name

BATF (Basic Leucine Zipper Transcriptional Factor ATF-like, B Cell-activating Transcription Factor, SF-HT-activated Gene 2 Protein) (HRP)

Gene Names

BATF; SFA2; B-ATF; BATF1; SFA-2

Reactivity

Human, Mouse, Rat

Applications

EIA, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of A431 cells using anti-YY1 antibody (AAA19673).Overlay histogram showing A431 cells stained with AAA19673 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-YY1 Antibody (AAA19673, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 8. IF analysis of YY1 using anti-YY1 antibody (M00179-1).YY1 was detected in an immunocytochemical section of T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL mouse anti-YY1 Antibody (M00179-1) overnight at 4 degree C. DyLight594 Conjugated Goat Anti-Mouse IgG (BA1141) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of YY1 using anti-YY1 antibody (AAA19673).YY1 was detected in a paraffin-embedded section of human the muscular layer of a colonic adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-YY1 Antibody (AAA19673) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of YY1 using anti-YY1 antibody (AAA19673).YY1 was detected in a paraffin-embedded section of human serous adenocarcinoma of ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-YY1 Antibody (AAA19673) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of YY1 using anti-YY1 antibody (AAA19673).YY1 was detected in a paraffin-embedded section of human pancreatic ductal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-YY1 Antibody (AAA19673) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of YY1 using anti-YY1 antibody (AAA19673).YY1 was detected in a paraffin-embedded section of human ovarian serous cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-YY1 Antibody (AAA19673) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of YY1 using anti-YY1 antibody (AAA19673).YY1 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinomas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-YY1 Antibody (AAA19673) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of YY1 using anti-YY1 antibody (AAA19673).YY1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-YY1 Antibody (AAA19673) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of YY1 using anti-YY1 antibody (AAA19673).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Caco-2 whole cell lysates,Lane 2: human SW620 whole cell lysates,Lane 3: human MDA-MB-453 lysates,Lane 4: human PC-3 whole cell lysates,Lane 5: rat thymus tissue lysates,Lane 6: mouse thymus tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-YY1 antigen affinity purified monoclonal antibody (#AAA19673) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for YY1 at approximately 65 kDa. The expected band size for YY1 is at 65 kDa.)

Compare (5 max)

YY1, Monoclonal Antibody (Cat# AAA19673)

Full Name

Anti-YY1 Antibody Picoband (monoclonal, 3F3E7)

Gene Names

YY1; DELTA; NF-E1; UCRBP; INO80S; YIN-YANG-1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ICC, IF, FC/FACS

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

< >

<< Previous Next >>

WB (Western Blot) (DNAJC10 monoclonal antibody. Western Blot analysis of DNAJC10 expression in HeLa.)

WB (Western Blot) (DNAJC10 monoclonal antibody. Western Blot analysis of DNAJC10 expression in Raw 264.7.)

Application Data (Detection limit for recombinant GST tagged DNAJC10 is ~10ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to DNAJC10 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to DNAJC10 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3ug/ml].)

WB (Western Blot) (DNAJC10 monoclonal antibody. Western Blot analysis of DNAJC10 expression in NIH/3T3.)

WB (Western Blot) (Western Blot detection against Immunogen (37.77kD).)

Compare (5 max)

DNAJC10, Monoclonal Antibody (Cat# AAA24489)

Full Name

DNAJC10 (DnaJ Homolog Subfamily C Member 10, ER-resident Protein ERdj5, Macrothioredoxin, MTHr, ERDJ5, UNQ495/PRO1012, DKFZp434J1813, MGC104194) APC

Gene Names

DNAJC10; JPDI; MTHr; ERdj5; PDIA19

Reactivity

Human, Mouse, Rat

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western Blot detection against Immunogen (77.26kD).)

IP (Immunoprecipitation) (Immunoprecipitation of STK38 transfected lysate using STK38 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with STK38 rabbit polyclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to STK38 on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to STK38 on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B tissue [antibody concentration 5ug/ml])

WB (Western Blot) (STK38 monoclonal antibody Western Blot analysis of STK38 expression in human kidney.)

WB (Western Blot) (Western Blot analysis of STK38 expression in transfected 293T cell line by STK38 monoclonal antibody Lane 1: STK38 transfected lysate (54.2kD). Lane 2: Non-transfected lysate.)

Compare (5 max)

STK38, Monoclonal Antibody (Cat# AAA24971)

Full Name

STK38 (Serine/Threonine-protein Kinase 38, NDR1 Protein Kinase, Nuclear Dbf2-related Kinase 1, NDR1) (Biotin)

Gene Names

STK38; NDR; NDR1

Reactivity

Human

Applications

EIA, IF, IHC, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (TBX3 monoclonal antibody (M02), clone 8H3. Western Blot analysis of TBX3 expression in PC-12 (Cat # L012V1).)

WB (Western Blot) (TBX3 monoclonal antibody (M02), clone 8H3. Western Blot analysis of TBX3 expression in NIH/3T3 (Cat # L018V1).)

Application Data (Detection limit for recombinant GST tagged TBX3 is approximately 0.03ng/ml as a capture antibody.)

WB (Western Blot) (TBX3 monoclonal antibody (M02), clone 8H3 Western Blot analysis of TBX3 expression in IMR-32 (Cat # L008V1).)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TBX3 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TBX3 on HeLa cell. [antibody concentration 10 ug/ml])

Compare (5 max)

TBX3, Monoclonal Antibody (Cat# AAA26305)

Full Name

TBX3 (T-Box 3, TBX3-ISO, UMS, XHL) (FITC)

Gene Names

TBX3; UMS; XHL; TBX3-ISO

Applications

IF, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of GABBR1 over-expressed 293 cell line, cotransfected with GABBR1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with GABBR1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged GABBR1 is ~0.3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GABBR1 on HeLa cell. [antibody concentration 10ug/ml].)

WB (Western Blot) (Western Blot analysis of GABBR1 expression in transfected 293T cell line by GABBR1 monoclonal antibody. Lane 1: GABBR1 transfected lysate (95kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (GABBR1 monoclonal antibody, Western Blot analysis of GABBR1 expression in IMR-32.)

WB (Western Blot) (Western Blot detection against Immunogen (36.63kD).)

Compare (5 max)

GABBR1, Monoclonal Antibody (Cat# AAA24515)

Full Name

GABBR1 (Gamma-aminobutyric Acid Type B Receptor Subunit 1, GABA-B Receptor 1, GABA-B-R1, GABA-BR1, GABABR1, Gb1) APC

Gene Names

GABBR1; GB1; GPRC3A; GABABR1; GABBR1-3

Reactivity

Human

Applications

EIA, IF, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (CMAS monoclonal antibody. Western Blot analysis of CMAS expression in Raw 264.7.)

WB (Western Blot) (CMAS monoclonal antibody, Western Blot analysis of CMAS expression in Hela NE.)

Application Data (Detection limit for recombinant GST tagged CMAS is ~1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CMAS on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CMAS on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3ug/ml].)

WB (Western Blot) (CMAS monoclonal antibody. Western Blot analysis of CMAS expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)

Compare (5 max)

CMAS, Monoclonal Antibody (Cat# AAA25060)

Full Name

CMAS (N-acylneuraminate Cytidylyltransferase, CMP-N-acetylneuraminic Acid Synthase, CMP-NeuNAc Synthase) (FITC)

Gene Names

CMAS; CSS

Reactivity

Human, Mouse, Rat

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between MAPK3 and RPS6KA2 HeLa cells were stained with anti-MAPK3 rabbit purified polyclonal 1:1200 and anti-RPS6KA2 mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

WB (Western Blot) (Western blot analysis of RPS6KA2 over-expressed 293 cell line, cotransfected with RPS6KA2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with RPS6KA2 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RPS6KA2 on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RPS6KA2 on formalin-fixed paraffin-embedded human cerebral cortex. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of RPS6KA2 expression in transfected 293T cell line by RPS6KA2 monoclonal antibody. Lane 1: RPS6KA2 transfected lysate (83.2kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (RPS6KA2 monoclonal antibody, Western Blot analysis of RPS6KA2 expression in Hela NE.)

Compare (5 max)

RSK3, Monoclonal Antibody (Cat# AAA25825)

Full Name

RSK3, CT (Ribosomal S6 Kinase 3, RSK-3, pp90RSK3, 90kD Ribosomal Protein S6 Kinase 2, p90-RSK 2, p90RSK2, MAP Kinase-activated Protein Kinase 1c, MAPK-activated Protein Kinase 1c, MAPKAP Kinase 1c, MAPKAPK1C, MAPKAPK-1c, Ribosomal Protein S6 Kinase alpha-

Gene Names

RPS6KA2; RSK; HU-2; RSK3; p90RSK2; p90-RSK3; pp90RSK3; MAPKAPK1C; S6K-alpha; S6K-alpha2

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (STK33 monoclonal antibody, Western Blot analysis of STK33 expression in HeLa.)

WB (Western Blot) (Western blot analysis of STK33 over-expressed 293 cell line, cotransfected with STK33 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with STK33 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

IP (Immunoprecipitation) (Immunoprecipitation of STK33 transfected lysate using STK33 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with STK33 rabbit polyclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to STK33 on HeLa cell. [antibody concentration 25ug/ml].)

WB (Western Blot) (Western Blot analysis of STK33 expression in transfected 293T cell line by STK33 monoclonal antibody. Lane 1: STK33 transfected lysate (57.9kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (82.65kD).)

Compare (5 max)

STK33, Monoclonal Antibody (Cat# AAA24970)

Full Name

STK33 (Serine/Threonine Kinase 33) (Biotin)

Reactivity

Human

Applications

EIA, IF, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

Application Data (Detection limit for recombinant GST tagged HDAC1 is approximately 0.1ng/ml as a capture antibody.)

WB (Western Blot) (HDAC1 monoclonal antibody (M06), clone 1D6 Western Blot analysis of HDAC1 expression in Hela S3 NE (Cat # L013V3).)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HDAC1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HDAC1 on HeLa cell. [antibody concentration 10 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HDAC1 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to HDAC1 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml])

Compare (5 max)

HDAC1, Monoclonal Antibody (Cat# AAA26319)

Full Name

HDAC1 (Histone Deacetylase 1, DKFZp686H12203, GON-10, HD1, RPD3, RPD3L1) (FITC)

Gene Names

HDAC1; HD1; RPD3; KDAC1; GON-10; RPD3L1

Applications

IF, IHC, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western Blot analysis of DHFR expression in HeLa.)

IF (Immunofluorescence) (Immunofluorescence on HeLa cells using 125814 (10ug/ml).)

Application Data (Detection limit for 125814 is ~0.1ng/ml as a capture antibody.)

WB (Western Blot) (Western Blot analysis of DHFR expression in transfected 293T cell line using 125814. Lane 1: DHFR transfected lysate (21.5kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot analysis of DHFR expression in PC-12 using 125814.)

WB (Western Blot) (Western Blot detection against immunogen (36.74kD).)

Compare (5 max)

DHFR, Monoclonal Antibody (Cat# AAA24781)

Full Name

DHFR (Dihydrofolate reductase) (Biotin)

Gene Names

DHFR; DYR; DHFRP1

Reactivity

Human, Rat

Applications

EIA, IF, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TTF2 on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (TTF2 monoclonal antibody (M06), clone 1E8. Western Blot analysis of TTF2 expression in PC-12.)

WB (Western Blot) (TTF2 monoclonal antibody (M06), clone 1E8. Western Blot analysis of TTF2 expression in Hela S3 NE.)

WB (Western Blot) (TTF2 monoclonal antibody (M06), clone 1E8. Western Blot analysis of TTF2 expression in Y-79.)

WB (Western Blot) (TTF2 monoclonal antibody (M06), clone 1E8 Western Blot analysis of TTF2 expression in NIH/3T3.)

WB (Western Blot) (TTF2 monoclonal antibody (M06), clone 1E8. Western Blot analysis of TTF2 expression in human placenta.)

Compare (5 max)

TTF2, Monoclonal Antibody (Cat# AAA26070)

Full Name

TTF2 (Transcription Termination Factor, RNA Polymerase II, HuF2) (APC)

Gene Names

TTF2; HuF2; ZGRF6

Applications

EIA, IF, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to POU5F1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to POU5F1 on HeLa cell. [antibody concentration 10 ug/ml])

Application Data (A-549 cells were stained with POU5F1-FITC labeled monoclonal antibody (Green). The cell nucleus were counterstained with DAPI (Blue).)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to POU5F1 on A-549 cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (Western Blot analysis of POU5F1 expression in transfected 293T cell line by POU5F1 monoclonal antibody (M05), clone 1B11.Lane 1: POU5F1 transfected lysate(18.3 KDa).Lane 2: Non-transfected lysate.)

WB (Western Blot) (POU5F1 monoclonal antibody (M05), clone 1B11 Western Blot analysis of POU5F1 expression in HepG2.)

Compare (5 max)

POU5F1, Monoclonal Antibody (Cat# AAA26096)

Full Name

POU5F1 (POU Class 5 Homeobox 1, MGC22487, OCT3, OCT4, OTF3, OTF4) (APC)

Applications

EIA, IF, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western Blot detection against Immunogen (84.74kD).)

WB (Western Blot) (PHGDH monoclonal antibody. Western Blot analysis of PHGDH expression in human liver.)

IP (Immunoprecipitation) (Immunoprecipitation of PHGDH transfected lysate using PHGDH monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PHGDH rabbit polyclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PHGDH on HeLa cell. [antibody concentration 1-10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PHGDH on formalin-fixed paraffin-embedded human prostate. [antibody concentration 3ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PHGDH on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 1-10ug/ml])

WB (Western Blot) (Western Blot analysis of PHGDH expression in transfected 293T cell line by PHGDH monoclonal antibody. Lane 1: PHGDH transfected lysate (56.7kD). Lane 2: Non-transfected lysate.)

Compare (5 max)

PHGDH, Monoclonal Antibody (Cat# AAA24314)

Full Name

PHGDH (D-3-phosphoglycerate Dehydrogenase, 3-PGDH, PGDH3, MGC3017, SERA) (AP)

Gene Names

PHGDH; NLS; PDG; PGD; NLS1; PGAD; PGDH; SERA; 3PGDH; 3-PGDH; PHGDHD; HEL-S-113

Reactivity

Human

Applications

EIA, IHC, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

Application Data (Detection limit for recombinant GST tagged TCF7L2 is approximately 0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TCF7L2 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TCF7L2 on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (TCF7L2 monoclonal antibody (M06), clone 3A4 Western Blot analysis of TCF7L2 expression in K-562 (Cat # L009V1).)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TCF7L2 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to TCF7L2 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml])

Compare (5 max)

TCF7L2, Monoclonal Antibody (Cat# AAA26619)

Full Name

TCF7L2 (Transcription Factor 7-like 2 (T-Cell Specific, HMG-Box), TCF-4, TCF4) (PE)

Gene Names

TCF7L2; TCF4; TCF-4

Applications

IF, IHC, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (CDC2 monoclonal antibody, Western Blot analysis of CDC2 expression in Hela.)

Application Data (Detection limit for recombinant GST tagged CDC2 is ~30ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CDC2 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CDC2 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 0.3ug/ml].)

WB (Western Blot) (Western Blot analysis of CDC2 expression in transfected 293T cell line by CDC2 monoclonal antibody. Lane 1: CDC2 transfected lysate (34.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (58.41kD).)

Compare (5 max)

CDC2, Monoclonal Antibody (Cat# AAA24753)

Full Name

CDC2 (Cell Division Control Protein 2 Homolog, CDC28A, Cell Division Protein Kinase 1, Cyclin-dependent Kinase 1, CDK1, p34 Protein Kinase, P34CDC2) (Biotin)

Gene Names

CDK1; CDC2; CDC28A; P34CDC2

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Detection of human CD20 by western blot. Samples: Whole cell lysate (25 ug) from Ramos and MCF-7 cells prepared using NETN lysis buffer. Antibody: Mouse anti-CD20 monoclonal antibody [L26] (AAA23779 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG . Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

IHC (Immunohistochemistry) (Detection of human CD20 (red) by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Mouse anti-CD20 monoclonal antibody [L26] (AAA23779 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-mouse IgG . Substrate: Opal. Counterstain: DAPI (blue).)

IHC (Immunohistochemistry) (Detection of human CD20 by immunhistochemistry. Sample: FFPE section of human tonsil. Antibody: Mouse monoclonal anti-CD20 antibody [L26] (AAA23779 lot 1) used at 1:250. Secondary: DyLight 594-conjugated goat anti-mouse IgG .)

IHC (Immunohistochemistry) (Detection of human CD20 by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Purified mouse monoclonal anti-CD20 (Clone L26) antibody (AAA23779 Lot2) used at a dilution of 1:500. Detection: DAB. Counterstain: IHC Hematoxylin (blue).)

ICC (Immunocytochemistry) (Detection of human CD20 by immunocytochemistry. Sample: FFPE section of VLN3G2 cells. Antibody: Purified mouse monoclonal anti-CD20 (Clone L26) antibody (AAA23779 Lot2) used at a dilution of 1:500. Detection: DAB. Counterstain: IHC Hematoxylin (blue).)

FCM (Flow Cytometry) (Detection of human CD20 (shaded) in Raji cells by flow cytometry. Antibody: Mouse anti-CD20 monoclonal antibody [L26] (AAA23779) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-mouse IgG .)

Compare (5 max)

CD20, Monoclonal Antibody (Cat# AAA23779)

Full Name

Mouse anti-CD20 Monoclonal Antibody, Purified [L26]

Gene Names

MS4A1; B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16

Reactivity

Human

Applications

WB, IHC, IHC-IF, FC/FACS, mIF

Purity

Purified

Pricing

$295/0.01 mL $680/0.1 mL $980/5x0.01 mL $1,300/2x0.1 mL $1,890/3x0.1 mL $2,440/4x0.1 mL $2,755/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of lysates from 293, Hela, mouse NIH/3T3, rat PC-12 cell line and rat brain tissue lysate(from left to right), using RPS6 Antibody (N-term). AAA28643 was diluted at 1:2000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded M. kidney section using RPS6 Antibody (N-term). AAA28643 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded H. kidney section using RPS6 Antibody (N-term). AAA28643 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded H. brain section using RPS6 Antibody (N-term). AAA28643 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)

IF (Immunofluorescence) (Fluorescent image of Hela cells stained with RPS6 Antibody (N-term). AAA28643 was diluted at 1:25 dilution. An Alexa Fluor 488-conjugated goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody (green). Cytoplasmic actin was counterstained with Alexa Fluor 555 conjugated with Phalloidin (red).)

FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells using RPS6 Antibody (N-term)(green) compared to an isotype control of mouse IgG1(blue). AAA28643 was diluted at 1:25 dilution. An Alexa Fluor 488 goat anti-mouse lgG at 1:400 dilution was used as the secondary antibody.)

Compare (5 max)

RPS6, Monoclonal Antibody (Cat# AAA28643)

Full Name

RPS6 Antibody (N-term)

Gene Names

RPS6; S6

Reactivity

Human, rat (Predicted Reactivity: Monkey, Mouse)

Applications

EIA, IHC, WB, FC/FACS, IF

Purity

Purified Mouse Monoclonal Antibody (Mab)

Pricing

$275/0.08 mL $510/0.4 mL $975/2x0.4 mL $1,415/3x0.4 mL $1,825/4x0.4 mL $2,070/5x0.4 mL

< >

<< Previous Next >>

WB (Western Blot) (Western Blot analysis of MPP1 expression in transfected 293T cell line by MPP1 monoclonal antibody (M01), clone 1E11-1G11.Lane 1: MPP1 transfected lysate (Predicted MW: 52.3 KDa).Lane 2: Non-transfected lysate.)

IP (Immunoprecipitation) (Immunoprecipitation of MPP1 transfected lysate using anti-MPP1 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with MPP1 monoclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MPP1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MPP1 on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (MPP1 monoclonal antibody (M01), clone 1E11-1G11. Western Blot analysis of MPP1 expression in human placenta.)

Application Data (Detection limit for recombinant GST tagged MPP1 is approximately 1ng/ml as a capture antibody.)

Compare (5 max)

MPP1, Monoclonal Antibody (Cat# AAA26079)

Full Name

MPP1 (Membrane Protein, Palmitoylated 1, 55kD, AAG12, DXS552, DXS552E, EMP55, MRG1, PEMP) (APC)

Gene Names

MPP1; MRG1; PEMP; AAG12; EMP55; DXS552E

Applications

IF, IP, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (NEK9 monoclonal antibody. Western Blot analysis of NEK9 expression in PC-12.)

WB (Western Blot) (NEK9 monoclonal antibody Western Blot analysis of NEK9 expression in HeLa.)

Application Data (Detection limit for recombinant GST tagged NEK9 is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NEK9 on HeLa cell. [antibody concentration 10ug/ml].)

WB (Western Blot) (Western Blot analysis of NEK9 expression in transfected 293T cell line by NEK9 monoclonal antibody. Lane 1: NEK9 transfected lysate (107.2kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (NEK9 monoclonal antibody. Western Blot analysis of NEK9 expression in NIH/3T3.)

WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)

Compare (5 max)

NEK9, Monoclonal Antibody (Cat# AAA24289)

Full Name

NEK9 (Serine/Threonine-protein Kinase Nek9, Nercc1 Kinase, Never in Mitosis A-related Kinase 9, NimA-related Protein Kinase 9, NimA-related Kinase 8, Nek8, KIAA1995, NEK8, NERCC, DKFZp434D0935, MGC138306, MGC16714, NERCC1) (AP)

Gene Names

NEK9; NC; APUG; NERCC; LCCS10; NERCC1

Reactivity

Human, Mouse, Rat

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

What are Monoclonal Antibodies?

Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.

This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.

AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.

What Are The Uses of Monoclonal Antibodies

Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.

ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.

Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.

Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.

Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.

How Monoclonal Antibodies Are Used as Medicine?

Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).

Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:

• Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.


• Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.


• Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.

Why Buy Monoclonal Antibodies From Us?

At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.

1. Validated for Versatile Applications

The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).

2. Wide Selection & Specialized Options

We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.

3. High-Quality Proteins

Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.

Frequently Asked Questions

1. Are your monoclonal antibodies validated for specific applications?

Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.

2. How do I choose the right monoclonal antibody for my application?

Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.

3. How quickly can I receive my order?

Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.