Monoclonal Antibodies

Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of U87 cells using anti-CTBP2 antibody (AAA19698).Overlay histogram showing U87 cells stained with AAA19698 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-CTBP2 Antibody (AAA19698, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 8. IHC analysis of CTBP2 using anti-CTBP2 antibody (AAA19698).CTBP2 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-CTBP2 Antibody (AAA19698) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of CTBP2 using anti-CTBP2 antibody (AAA19698).CTBP2 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-CTBP2 Antibody (AAA19698) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of CTBP2 using anti-CTBP2 antibody (AAA19698).CTBP2 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-CTBP2 Antibody (AAA19698) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of CTBP2 using anti-CTBP2 antibody (AAA19698).CTBP2 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-CTBP2 Antibody (AAA19698) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of CTBP2 using anti-CTBP2 antibody (AAA19698).CTBP2 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-CTBP2 Antibody (AAA19698) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of CTBP2 using anti-CTBP2 antibody (AAA19698).CTBP2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-CTBP2 Antibody (AAA19698) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of CTBP2 using anti-CTBP2 antibody (AAA19698).CTBP2 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-CTBP2 Antibody (AAA19698) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of CTBP2 using anti-CTBP2 antibody (AAA19698).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human SiHa whole cell lysates,Lane 3: human COLO 320 whole cell lysates,Lane 4: human SW620 whole cell ysates,Lane 5: rat stomach tissue lysates,Lane 6: rat brain tissue lysates,Lane 7: mouse stomach tissue lysates,Lane 8: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CTBP2 antigen affinity purified monoclonal antibody (#AAA19698) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CTBP2 at approximately 49 kDa. The expected band size for CTBP2 is at 49 kDa.)

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CTBP2, Monoclonal Antibody (Cat# AAA19698)

Full Name

Anti-CTBP2 Antibody Picoband (monoclonal, 7F3E1)

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, FC/FACS

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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WB (Western Blot) (Western Blot detection against Immunogen (54.71kD).)

WB (Western Blot) (TARDBP monoclonal antibody Western Blot analysis of TARDBP expression in A-431.)

WB (Western Blot) (Western blot analysis of TARDBP over-expressed 293 cell line, cotransfected with TARDBP Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TARDBP monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged TARDBP is 0.3ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of TARDBP transfected lysate using TARDBP monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with TARDBP rabbit polyclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TARDBP on HeLa cell. [antibody concentration 10ug/ml])

WB (Western Blot) (Western Blot analysis of TARDBP expression in transfected 293T cell line by TARDBP monoclonal antibody. Lane 1: TARDBP transfected lysate (44.7kD). Lane 2: Non-transfected lysate.)

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TARDBP, Monoclonal Antibody (Cat# AAA24976)

Full Name

TARDBP (TAR DNA-binding Protein 43, TDP43, TDP-43, ALS10) (Biotin)

Gene Names

TARDBP; ALS10; TDP-43

Reactivity

Human

Applications

EIA, IF, IHC, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (BATF monoclonal antibody Western Blot analysis of BATF expression in Hela NE.)

WB (Western Blot) (BATF monoclonal antibody Western Blot analysis of BATF expression in PC-12.)

Application Data (Detection limit for recombinant GST tagged BATF is ~1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of BATF transfected lysate using BATF monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with BATF rabbit polyclonal antibody.)

WB (Western Blot) (Western Blot analysis of BATF expression in transfected 293T cell line by BATF monoclonal antibody Lane 1: BATF transfected lysate (14.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (BATF monoclonal antibody Western Blot analysis of BATF expression in NIH/3T3.)

WB (Western Blot) (Western Blot detection against Immunogen (36.23kD).)

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BATF, Monoclonal Antibody (Cat# AAA24737)

Full Name

BATF (Basic Leucine Zipper Transcriptional Factor ATF-like, B Cell-activating Transcription Factor, SF-HT-activated Gene 2 Protein) (Biotin)

Gene Names

BATF; SFA2; B-ATF; BATF1; SFA-2

Reactivity

Human, Mouse, Rat

Applications

EIA, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western blot analysis of PAX5 over-expressed 293 cell line, cotransfected with PAX5 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PAX5 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for 130905 is ~0.03ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase on formalin-fixed paraffin-embedded human tonsil using 130905 (1.5ug/ml).)

WB (Western Blot) (Western Blot analysis of PAX5 expression in transfected 293T cell line by 130905. Lane 1: PAX5 transfected lysate (42.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot analysis of PAX5 expression in IMR-32 using 130905.)

WB (Western Blot) (Western Blot detection against immunogen (37.84kD).)

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PAX5, Monoclonal Antibody (Cat# AAA25780)

Full Name

PAX5 (Paired Box Protein Pax-5, B Cell-specific Transcription Factor, BSAP, PAX-5) (PE)

Gene Names

PAX5; BSAP

Reactivity

Human

Applications

IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (MTA1 monoclonal antibody (M02), clone 4D11. Western Blot analysis of MTA1 expression in PC-12.)

Application Data (Detection limit for recombinant GST tagged MTA1 is approximately 3ng/ml as a capture antibody.)

WB (Western Blot) (MTA1 monoclonal antibody (M02), clone 4D11. Western Blot analysis of MTA1 expression in NIH/3T3.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MTA1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MTA1 on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (MTA1 monoclonal antibody (M02), clone 4D11 Western Blot analysis of MTA1 expression in Hela S3 NE.)

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MTA1, Monoclonal Antibody (Cat# AAA26045)

Full Name

MTA1 (Metastasis Associated 1) (APC)

Applications

IF, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (EFHD1 monoclonal antibody (M05), Western Blot analysis of EFHD1 expression in HeLa.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to EFHD1 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3ug/ml].)

WB (Western Blot) (EFHD1 monoclonal antibody (M05). Western Blot analysis of EFHD1 expression in NIH/3T3.)

WB (Western Blot) (EFHD1 monoclonal antibody. Western Blot analysis of EFHD1 expression in Raw 264.7.)

WB (Western Blot) (EFHD1 monoclonal antibody. Western Blot analysis of EFHD1 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (33.81kD).)

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EFHD1, Monoclonal Antibody (Cat# AAA24196)

Full Name

EFHD1 (EF-hand Domain-containing Protein D1, EF-hand Domain-containing Protein 1, Swiprosin-2, SWS2, PP3051) (AP)

Gene Names

EFHD1; SWS2; MST133; PP3051; MSTP133; FLJ13612; DKFZp781H0842

Reactivity

Human, Mouse, Rat

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged STIP1 is approximately 0.03ng/ml as a capture antibody.)

WB (Western Blot) (STIP1 monoclonal antibody (M11), clone 1E3 Western Blot analysis of STIP1 expression in HeLa (Cat # L013V1).)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to STIP1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to STIP1 on HeLa cell. [antibody concentration 10 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to STIP1 on formalin-fixed paraffin-embedded human lung. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to STIP1 on formalin-fixed paraffin-embedded human lung. [antibody concentration 3 ug/ml])

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STIP1, Monoclonal Antibody (Cat# AAA26316)

Full Name

STIP1 (Stress-Induced-Phosphoprotein 1, HOP, IEF-SSP-3521, P60, STI1, STI1L) (FITC)

Gene Names

STIP1; HOP; P60; STI1; STI1L; HEL-S-94n; IEF-SSP-3521

Applications

IF, IHC, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (TBX3 monoclonal antibody (M02), clone 8H3. Western Blot analysis of TBX3 expression in PC-12 (Cat # L012V1).)

WB (Western Blot) (TBX3 monoclonal antibody (M02), clone 8H3. Western Blot analysis of TBX3 expression in NIH/3T3 (Cat # L018V1).)

Application Data (Detection limit for recombinant GST tagged TBX3 is approximately 0.03ng/ml as a capture antibody.)

WB (Western Blot) (TBX3 monoclonal antibody (M02), clone 8H3 Western Blot analysis of TBX3 expression in IMR-32 (Cat # L008V1).)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TBX3 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TBX3 on HeLa cell. [antibody concentration 10 ug/ml])

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TBX3, Monoclonal Antibody (Cat# AAA26571)

Full Name

TBX3 (T-Box 3, TBX3-ISO, UMS, XHL) (PE)

Gene Names

TBX3; UMS; XHL; TBX3-ISO

Applications

IF, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of VRK2 expression in transfected 293T cell line by VRK2 monoclonal antibody Lane 1: VRK2 transfected lysate (58.1kD). Lane 2: Non-transfected lysate.)

Application Data (Detection limit for recombinant GST tagged VRK2 is ~1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to VRK2 on HeLa cell. [antibody concentration 10ug/ml])

WB (Western Blot) (Western blot analysis of VRK2 over-expressed 293 cell line, cotransfected with VRK2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with VRK2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

WB (Western Blot) (VRK2 monoclonal antibody, Western Blot analysis of VRK2 expression in K-562.)

WB (Western Blot) (VRK2 monoclonal antibody. Western Blot analysis of VRK2 expression in U-2 OS.)

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VRK2, Monoclonal Antibody (Cat# AAA25294)

Full Name

VRK2 (Vaccinia-related Kinase 2, Serine/threonine-protein Kinase VRK2) (FITC)

Reactivity

Human

Applications

EIA, IF, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of B2M expression in transfected 293T cell line by B2M monoclonal antibody. Lane 1: B2M transfected lysate (13.7kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (B2M monoclonal antibody, Western Blot analysis of B2M expression in U-2 OS.)

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between CALR and B2M. HeLa cells were stained with CALR rabbit purified polyclonal 1:1200 and B2M mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Application Data (Detection limit for recombinant GST tagged B2M is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to B2M on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to B2M on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western blot analysis of B2M over-expressed 293 cell line, cotransfected with B2M Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with B2M monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

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b2-Microglobulin, Monoclonal Antibody (Cat# AAA24275)

Full Name

b2-Microglobulin (B2M, Beta-2-microglobulin) (AP)

Gene Names

B2M; IMD43

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged SRPK1 is ~0.03ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SRPK1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1ug/ml].)

WB (Western Blot) (Western Blot analysis of SRPK1 expression in transfected 293T cell line by SRPK1 monoclonal antibody. Lane 1: SRPK1 transfected lysate (74.3kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (SRPK1 monoclonal antibody, Western Blot analysis of SRPK1 expression in IMR-32.)

WB (Western Blot) (SRPK1 monoclonal antibody. Western Blot analysis of SRPK1 expression in HepG2.)

WB (Western Blot) (Western Blot detection against Immunogen (37kD).)

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SRPK1, Monoclonal Antibody (Cat# AAA25557)

Full Name

SRPK1 (SRSF Protein Kinase 1, SFRS Protein Kinase 1, Serine/Arginine-rich Protein-specific Kinase 1, SR-protein-specific Kinase 1) (HRP)

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (EIF2S2 monoclonal antibody. Western Blot analysis of EIF2S2 expression in HeLa.)

WB (Western Blot) (EIF2S2 monoclonal antibody. Western Blot analysis of EIF2S2 expression in PC-12.)

Application Data (Detection limit for recombinant GST tagged EIF2S2 is 0.3ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to EIF2S2 on formalin-fixed paraffin-embedded human pancreatic cancer. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of EIF2S2 expression in transfected 293T cell line by EIF2S2 monoclonal antibody. Lane 1: EIF2S2 transfected lysate (38.4kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (EIF2S2 monoclonal antibody. Western Blot analysis of EIF2S2 expression in NIH/3T3.)

WB (Western Blot) (EIF2S2 monoclonal antibody. Western Blot analysis of EIF2S2 expression in Raw 264.7.)

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EIF2S2, Monoclonal Antibody (Cat# AAA24790)

Full Name

EIF2S2 (EIF2B, Eukaryotic Translation Initiation Factor 2 Subunit 2, Eukaryotic Translation Initiation Factor 2 Subunit beta, DKFZp686L18198, EIF2Beta, MGC8508) (Biotin)

Gene Names

EIF2S2; EIF2; EIF2B; PPP1R67; EIF2beta; eIF-2-beta

Reactivity

Human, Mouse, Rat

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged PCAF is approximately 3ng/ml as a capture antibody.)

WB (Western Blot) (PCAF monoclonal antibody (M06), clone 5E5. Western Blot analysis of PCAF expression in human uterus myoma.)

WB (Western Blot) (PCAF monoclonal antibody (M06), clone 5E5. Western Blot analysis of PCAF expression in C32 (Cat # L002V1).)

WB (Western Blot) (PCAF monoclonal antibody (M06), clone 5E5. Western Blot analysis of PCAF expression in A-431 (Cat # L015V1).)

WB (Western Blot) (PCAF monoclonal antibody (M06), clone 5E5 Western Blot analysis of PCAF expression in Hela S3 NE (Cat # L013V3).)

WB (Western Blot) (PCAF monoclonal antibody (M06), clone 5E5. Western Blot analysis of PCAF expression in human thyroid (diffuse hyperplasia).)

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PCAF, Monoclonal Antibody (Cat# AAA26608)

Full Name

PCAF (K(Lysine) Acetyltransferase 2B, CAF, P, P/CAF, PCAF) (PE)

Gene Names

KAT2B; CAF; PCAF; P/CAF

Applications

IF, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Proximity Ligation Analysis of protein-protein interactions between TP53 and MDM2 HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-MDM2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)

WB (Western Blot) (Western blot analysis of MDM2 over-expressed 293 cell line, cotransfected with MDM2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MDM2 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged MDM2 is ~0.03ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to MDM2 on formalin-fixed paraffin-embedded human cerebral cortex. [antibody concentration 1.5ug/ml])

WB (Western Blot) (Western Blot analysis of MDM2 expression in transfected 293T cell line by MDM2 monoclonal antibody. Lane 1: MDM2 transfected lysate (55.2kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

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MDM2, Monoclonal Antibody (Cat# AAA24565)

Full Name

MDM2 (E3 Ubiquitin-protein Ligase Mdm2, Double Minute 2 Protein, Hdm2, Oncoprotein Mdm2, p53-binding Protein Mdm2) APC

Gene Names

MDM2; HDMX; hdm2; ACTFS

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IF (Immunofluorescence) (Figure 7. IF analysis of NKCC1/SLC12A2 using anti-NKCC1/SLC12A2 antibody (AAA19708).NKCC1/SLC12A2 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 ug/mL mouse anti-NKCC1/SLC12A2 Antibody (AAA19708) overnight at 4 degree C. Biotin conjugated goat anti-mouse IgG (BA1001) was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using DyLight550 Conjugated Avidin (BA1134). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IF (Immunofluorescence) (Figure 6. IF analysis of NKCC1/SLC12A2 using anti-NKCC1/SLC12A2 antibody (AAA19708).NKCC1/SLC12A2 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 ug/mL mouse anti-NKCC1/SLC12A2 Antibody (AAA19708) overnight at 4 degree C. Biotin conjugated goat anti-mouse IgG (BA1001) was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using DyLight550 Conjugated Avidin (BA1134). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IF (Immunofluorescence) (Figure 5. IF analysis of NKCC1/SLC12A2 using anti-NKCC1/SLC12A2 antibody (AAA19708).NKCC1/SLC12A2 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 ug/mL mouse anti-NKCC1/SLC12A2 Antibody (AAA19708) overnight at 4 degree C. Biotin conjugated goat anti-mouse IgG (BA1001) was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using DyLight550 Conjugated Avidin (BA1134). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of NKCC1/SLC12A2 using anti-NKCC1/SLC12A2 antibody (AAA19708).NKCC1/SLC12A2 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-NKCC1/SLC12A2 Antibody (AAA19708) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of NKCC1/SLC12A2 using anti-NKCC1/SLC12A2 antibody (AAA19708).NKCC1/SLC12A2 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-NKCC1/SLC12A2 Antibody (AAA19708) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of NKCC1/SLC12A2 using anti-NKCC1/SLC12A2 antibody (AAA19708).NKCC1/SLC12A2 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-NKCC1/SLC12A2 Antibody (AAA19708) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of NKCC1/SLC12A2 using anti-NKCC1/SLC12A2 antibody (AAA19708).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human A549 whole cell lysates,Lane 2: human CACO-2 whole cell lysates,Lane 3: human PC-3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-NKCC1/SLC12A2 antigen affinity purified monoclonal antibody (#AAA19708) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for NKCC1/SLC12A2 at approximately 200 kDa. The expected band size for NKCC1/SLC12A2 is at 131 kDa.)

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NKCC1/SLC12A2, Monoclonal Antibody (Cat# AAA19708)

Full Name

Anti-NKCC1/SLC12A2 Antibody Picoband (monoclonal, 6G7D2)

Gene Names

SLC12A2; BSC; BSC2; NKCC1; PPP1R141

Reactivity

Human, Mouse, Rat

Applications

IF, IHC, WB

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of RH35 cells using anti- EIF4A1 antibody (AAA19380).Overlay histogram showing RH35 cells stained with AAA19380 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-EIF4A1 Antibody (AAA19380, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of HEPA1-6 cells using anti- EIF4A1 antibody (AAA19380).Overlay histogram showing HEPA1-6 cells stained with AAA19380 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-EIF4A1 Antibody (AAA19380, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

FCM (Flow Cytometry) (Figure 5. Flow Cytometry analysis of CACO-2 cells using anti- EIF4A1 antibody (AAA19380).Overlay histogram showing CACO-2 cells stained with AAA19380 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-EIF4A1 Antibody (AAA19380, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 4. IF analysis of EIF4A1 using anti- EIF4A1 antibody (AAA19380).EIF4A1 was detected in immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti- EIF4A1 Antibody (AAA19380) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of EIF4A1 using anti-EIF4A1 antibody (AAA19380).EIF4A1 was detected in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-EIF4A1 Antibody (AAA19380) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of EIF4A1 using anti-EIF4A1 antibody (AAA19380).EIF4A1 was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-EIF4A1 Antibody (AAA19380) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of EIF4A1 using anti-EIF4A1 antibody (AAA19380).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hela whole cell lysatesLane 2: human Jurkat whole cell lysatesLane 3: human MCF-7 whole cell lysatesLane 4: human HEK293 whole cell lysatesLane 5: human HEPG2 whole cell lysatesLane 6: human K562 whole cell lysatesLane 7: rat PC-12 whole cell lysatesLane 8: mouse spleen tissue lysatesLane 9: mouse lung tissue lysatesLane 10: mouse RAW264. 7 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti-EIF4A1 antigen affinity purified monoclonal antibody (Catalog # AAA19380) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for EIF4A1 at approximately 46KD. The expected band size for EIF4A1 is at 46KD.)

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EIF4A1, Monoclonal Antibody (Cat# AAA19380)

Full Name

Anti-EIF4A1 Antibody (monoclonal, 11B8)

Gene Names

EIF4A1; DDX2A; EIF4A; EIF-4A; eIF4A-I; eIF-4A-I

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, ICC, IF, FC/FACS/FCM

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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Application Data (Detection limit for recombinant GST tagged NKX3-1 is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NKX3-1 on NIH/3T3 cell. [antibody concentration 10ug/ml])

WB (Western Blot) (NKX3-1 monoclonal antibody. Western Blot analysis of NKX3-1 expression in NIH/3T3.)

WB (Western Blot) (NKX3-1 monoclonal antibody. Western Blot analysis of NKX3-1 expression in Raw 264.7.)

WB (Western Blot) (NKX3-1 monoclonal antibody. Western Blot analysis of NKX3-1 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)

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NKX3-1, Monoclonal Antibody (Cat# AAA25768)

Full Name

NKX3-1 (Homeobox Protein Nkx-3.1, Homeobox Protein NK-3 Homolog A, NKX3.1, NKX3A) (PE)

Gene Names

NKX3-1; NKX3; BAPX2; NKX3A; NKX3.1

Reactivity

Human, Mouse, Rat

Applications

EIA, IF, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western blot analysis of PDK2 over-expressed 293 cell line, cotransfected with PDK2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PDK2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged PDK2 is ~0.3ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PDK2 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 0.8ug/ml])

WB (Western Blot) (Western Blot analysis of PDK2 expression in transfected 293T cell line by PDK2 monoclonal antibody. Lane 1: PDK2 transfected lysate (46.2kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (PDK2 monoclonal antibody Western Blot analysis of PDK2 expression in U-2 OS.)

WB (Western Blot) (Western Blot detection against Immunogen (35.31kD).)

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PDHK2, Monoclonal Antibody (Cat# AAA25785)

Full Name

PDHK2 [Pyruvate Dehydrogenase [Lipoamide]] Kinase Isozyme 2, Mitochondrial, Pyruvate Dehydrogenase Kinase Isoform 2, PDH Kinase 2, PDKII, PDK2) (PE)

Gene Names

PDK2; PDHK2; PDKII

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of CSTF3 expression in transfected 293T cell line by CSTF3 monoclonal antibody. Lane 1: CSTF3 transfected lysate (11.44kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western blot analysis of CSTF3 over-expressed 293 cell line, cotransfected with CSTF3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CSTF3 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged CSTF3 is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CSTF3 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CSTF3 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot detection against Immunogen (37.07kD).)

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CSTF3, Monoclonal Antibody (Cat# AAA24771)

Full Name

CSTF3 (Cleavage Stimulation Factor Subunit 3, CF-1 77kD Subunit, Cleavage Stimulation Factor 77kD Subunit, CSTF 77kD Subunit, CstF-77) (Biotin)

Gene Names

CSTF3; CSTF-77

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (BANF1 monoclonal antibody (M07), clone M2. Western Blot analysis of BANF1 expression in PC-12.)

WB (Western Blot) (BANF1 monoclonal antibody (M07), clone M2. Western Blot analysis of BANF1 expression in Raw 264.7.)

WB (Western Blot) (BANF1 monoclonal antibody (M07), clone M2. Western Blot analysis of BANF1 expression in Jurkat.)

Application Data (Detection limit for recombinant GST tagged BANF1 is approximately 10ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to BANF1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to BANF1 on HeLa cell. [antibody concentration 10 ug/ml])

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BANF1, Monoclonal Antibody (Cat# AAA26310)

Full Name

BANF1 (Barrier to Autointegration Factor 1, BAF, BCRP1, D14S1460, MGC111161) (FITC)

Gene Names

BANF1; BAF; NGPS; BCRP1; D14S1460

Applications

IF, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of B2M expression in transfected 293T cell line by B2M monoclonal antibody. Lane 1: B2M transfected lysate (13.7kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (B2M monoclonal antibody, Western Blot analysis of B2M expression in U-2 OS.)

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between CALR and B2M. HeLa cells were stained with CALR rabbit purified polyclonal 1:1200 and B2M mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Application Data (Detection limit for recombinant GST tagged B2M is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to B2M on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to B2M on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western blot analysis of B2M over-expressed 293 cell line, cotransfected with B2M Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with B2M monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

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b2-Microglobulin, Monoclonal Antibody (Cat# AAA24571)

Full Name

b2-Microglobulin (B2M, Beta-2-microglobulin) (APC)

Gene Names

B2M; IMD43

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (CFL1 monoclonal antibody. Western Blot analysis of CFL1 expression in PC-12.)

WB (Western Blot) (CFL1 monoclonal antibody. Western Blot analysis of CFL1 expression in Raw 264.7.)

Application Data (Detection limit for recombinant GST tagged CFL1 is ~10ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CFL1 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CFL1 on formalin-fixed paraffin-embedded human breast cancer. [antibody concentration 1.5ug/ml].)

WB (Western Blot) (CFL1 monoclonal antibody. Western Blot analysis of CFL1 expression in NIH/3T3.)

WB (Western Blot) (Western Blot detection against Immunogen (44kD).)

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CFL1, Monoclonal Antibody (Cat# AAA24464)

Full Name

CFL1 (Cofilin-1, Cofilin, Non-muscle Isoform, 18kD Phosphoprotein, p18, CFL) APC

Gene Names

CFL1; CFL; cofilin; HEL-S-15

Reactivity

Human, Mouse

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (SPRY2 monoclonal antibody Western Blot analysis of SPRY2 expression in C32)

Application Data (Detection limit for recombinant GST tagged SPRY2 is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SPRY2 on HeLa cell. [antibody concentration 25ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SPRY2 on formalin-fixed paraffin-embedded human lymphoma. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of SPRY2 expression in transfected 293T cell line by SPRY2 monoclonal antibody Lane 1: SPRY2 transfected lysate (34.7kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (60.76kD).)

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SPRY2, Monoclonal Antibody (Cat# AAA25262)

Full Name

SPRY2 (Protein Sprouty Homolog 2, Spry-2, hSPRY2) (FITC)

Gene Names

SPRY2; IGAN3; hSPRY2

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (AKT3 monoclonal antibody (M03), clone 3F3. Western Blot analysis of AKT3 expression in PC-12.)

WB (Western Blot) (AKT3 monoclonal antibody (M03), clone 3F3. Western Blot analysis of AKT3 expression in Raw 264.7.)

WB (Western Blot) (AKT3 monoclonal antibody (M03), clone 3F3. Western Blot analysis of AKT3 expression in NIH/3T3.)

Application Data (Detection limit for recombinant GST tagged AKT3 is approximately 0.03ng/ml as a capture antibody.)

WB (Western Blot) (AKT3 monoclonal antibody (M03), clone 3F3. Western Blot analysis of AKT3 expression in MCF-7.)

WB (Western Blot) (AKT3 monoclonal antibody (M03), clone 3F3 Western Blot analysis of AKT3 expression in HeLa.)

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AKT3, Monoclonal Antibody (Cat# AAA26046)

Full Name

AKT3 (v-akt murine Thymoma Viral Oncogene Homolog 3 (Protein Kinase B, gamma), DKFZp434N0250, PKB-GAMMA, PKBG, PRKBG, RAC-PK-gamma, RAC-gamma, STK-2) (APC)

Gene Names

AKT3; MPPH; PKBG; MPPH2; PRKBG; STK-2; PKB-GAMMA; RAC-gamma; RAC-PK-gamma

Applications

WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (CHEK2 monoclonal antibody Western Blot analysis of CHEK2 expression in HeLa NE.)

Application Data (Detection limit for recombinant GST tagged CHEK2 is ~3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CHEK2 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CHEK2 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml].)

WB (Western Blot) (CHEK2 monoclonal antibody Western Blot analysis of CHEK2 expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (85.84kD).)

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CHEK2, Monoclonal Antibody (Cat# AAA24760)

Full Name

CHEK2 (Serine/Threonine-protein Kinase Chk2, CHK2 Checkpoint Homolog, Cds1 Homolog, Hucds1, hCds1, Checkpoint Kinase 2, CDS1, CHK2, RAD53) (Biotin)

Gene Names

CHEK2; CDS1; CHK2; LFS2; RAD53; hCds1; HuCds1; PP1425

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IP (Immunoprecipitation) (Immunoprecipitation of GART transfected lysate using GART monoclonal antibody and Protein A Magnetic Bead and immunoblotted with GART rabbit polyclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GART on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GART on formalin-fixed paraffin-embedded human endometrium tissue. [antibody concentration 2ug/ml].)

WB (Western Blot) (Western Blot analysis of GART expression in transfected 293T cell line by GART monoclonal antibody. Lane 1: GART transfected lysate (46kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (GART monoclonal antibody, Western Blot analysis of GART expression in HeLa NE.)

WB (Western Blot) (Western Blot detection against Immunogen (73.37kD).)

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GART, Monoclonal Antibody (Cat# AAA24516)

Full Name

GART (Trifunctional Purine Biosynthetic Protein Adenosine-3, PGFT, PRGS, MGC47764) APC

Gene Names

GART; AIRS; GARS; PAIS; PGFT; PRGS; GARTF

Reactivity

Human

Applications

EIA, IF, IHC, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (CPSF3 monoclonal antibody. Western Blot analysis of CPSF3 expression in NIH/3T3.)

WB (Western Blot) (Western Blot analysis of CPSF3 expression in transfected 293T cell line by CPSF3 monoclonal antibody. Lane 1: CPSF3 transfected lysate (77.5kD). Lane 2: Non-transfected lysate.)

Application Data (Detection limit for recombinant GST tagged CPSF3 is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CPSF3 on HeLa cell. [antibody concentration 10ug/ml].)

WB (Western Blot) (CPSF3 monoclonal antibody, Western Blot analysis of CPSF3 expression in Hela NE.)

WB (Western Blot) (CPSF3 monoclonal antibody. Western Blot analysis of CPSF3 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)

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CPSF3, Monoclonal Antibody (Cat# AAA14795)

Full Name

CPSF3 (Cleavage and Polyadenylation Specificity Factor Subunit 3, Cleavage and Polyadenylation Specificity Factor 73 kDa Subunit, CPSF 73 kDa Subunit, mRNA 3'-end-processing Endonuclease CPSF-73, CPSF73)

Gene Names

CPSF3; CPSF73; CPSF-73

Reactivity

Human, Mouse, Rat

Applications

EL/EIA, WB, IF

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

$820/0.1 mg $1,565/2x0.1 mg $2,270/3x0.1 mg $2,940/4x0.1 mg $3,380/5x0.1 mg

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IHC (Immunohistochemistry) (Dilution: WB: 1:2000 IF: 1:100-200 IHC 1:50-300)

IF (Immunofluorescence) (Dilution: WB: 1:2000 IF: 1:100-200 IHC 1:50-300)

Application Data (Dilution: WB: 1:2000 IF: 1:100-200 IHC 1:50-300)

IF (Immunofluorescence) (Dilution: WB: 1:2000 IF: 1:100-200 IHC 1:50-300)

IHC (Immunohistochemistry-Paraffin) (Dilution: WB: 1:2000 IF: 1:100-200 IHC 1:50-300)

IHC (Immunohistochemistry-Paraffin) (Dilution: WB: 1:2000 IF: 1:100-200 IHC 1:50-300)

WB (Western Blot) (Dilution: WB: 1:2000 IF: 1:100-200 IHC 1:50-300)

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EFHD1, Monoclonal Antibody (Cat# AAA28880)

Full Name

EFHD1 Monoclonal Antibody (3G2)

Gene Names

EFHD1; SWS2; MST133; PP3051; MSTP133

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, IF

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

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Application Data (Proximity Ligation Analysis of protein-protein interactions between CDC6 and MCM7. Huh7 cells were stained with anti-CDC6 rabbit purified polyclonal 1:600 and anti-MCM7 mouse monoclonal antibody 1:100. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)

Application Data (Proximity Ligation Analysis of protein-protein interactions between CDK7 and MCM7 HeLa cells were stained with anti-CDK7 rabbit purified polyclonal 1:1200 and anti-MCM7 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)

Application Data (Detection limit for recombinant GST tagged MCM7 is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MCM7 on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to MCM7 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. [antibody concentration 3ug/ml])

WB (Western Blot) (MCM7 monoclonal antibody Western Blot analysis of MCM7 expression in Hela NE.)

WB (Western Blot) (Western Blot detection against Immunogen (68.53kD).)

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MCM7, Monoclonal Antibody (Cat# AAA24268)

Full Name

MCM7 (DNA Replication Licensing Factor MCM7, CDC47 Homolog, P1.1-MCM3, CDC47, MCM2) (AP)

Gene Names

MCM7; MCM2; CDC47; P85MCM; P1CDC47; PNAS146; PPP1R104; P1.1-MCM3

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged MEIS2 is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MEIS2 on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to MEIS2 on formalin-fixed paraffin-embedded human spleen tissue. [antibody concentration 1ug/ml])

WB (Western Blot) (Western Blot analysis of MEIS2 expression in transfected 293T cell line by MEIS2 monoclonal antibody. Lane 1: MEIS2 transfected lysate (51.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (MEIS2 monoclonal antibody Western Blot analysis of MEIS2 expression in K-562.)

WB (Western Blot) (MEIS2 monoclonal antibody Western Blot analysis of MEIS2 expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (67.65kD).)

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MEIS2, Monoclonal Antibody (Cat# AAA24271)

Full Name

MEIS2 (Homeobox Protein Meis2, Meis1-related Protein 1, MRG1, MGC2820) (AP)

Gene Names

MEIS2; MRG1; HsT18361

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (RRAS2 monoclonal antibody Western Blot analysis of RRAS2 expression in A-431.)

WB (Western Blot) (RRAS2 monoclonal antibody Western Blot analysis of RRAS2 expression in NIH/3T3)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RRAS2 on A-431 cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RRAS2 on formalin-fixed paraffin-embedded human dysgerminoma tissue. [antibody concentration 5ug/ml])

WB (Western Blot) (Western Blot analysis of RRAS2 expression in transfected 293T cell line by RRAS2 monoclonal antibody Lane 1: RRAS2 transfected lysate (Predicted MW: 23.4kD. Lane 2: Non-transfected lysate.)

WB (Western Blot) (RRAS2 monoclonal antibody Western Blot analysis of RRAS2 expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (48.55kD).)

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RRAS2, Monoclonal Antibody (Cat# AAA25824)

Full Name

RRAS2 (Ras-related Protein R-Ras2, Ras-like Protein TC21, Teratocarcinoma Oncogene, TC21) (PE)

Reactivity

Human, Mouse, Rat

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged GCH1 is ~0.3ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of GCH1 transfected lysate using 127227and Protein A Magnetic Bead and immunoblotted with GCH1 rabbit polyclonal antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase on formalin-fixed paraffin-embedded human lymph node using 127227 (3ug/ml).)

WB (Western Blot) (Western Blot analysis of GCH1 expression in transfected 293T cell line using 127227. Lane 1: GCH1 transfected lysate (27.9kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot analysis of GCH1 using 127227 expression in IMR-32.)

WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)

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GCH1, Monoclonal Antibody (Cat# AAA24814)

Full Name

GCH1 (GTP Cyclohydrolase 1, GTP Cyclohydrolase I, GTP-CH-I, DYT5, GCH) (Biotin)

Gene Names

GCH1; GCH; DYT5; DYT14; DYT5a; GTPCH1; HPABH4B; GTP-CH-1

Reactivity

Human

Applications

EIA, IHC, IP, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (SAV1 monoclonal antibody (M02), Western Blot analysis of SAV1 expression in Hela NE.)

Application Data (Detection limit for recombinant GST tagged SAV1 is ~0.1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of SAV1 transfected lysate using SAV1 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with rabbit polyclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SAV1 on HeLa cell. [antibody concentration 60ug/ml].)

WB (Western Blot) (SAV1 monoclonal antibody. Western Blot analysis of SAV1 expression in HepG2.)

WB (Western Blot) (Western Blot detection against Immunogen (35.35kD).)

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SAV1, Monoclonal Antibody (Cat# AAA25831)

Full Name

SAV1 (Protein Salvador Homolog 1, SAV, 45kD WW Domain Protein, WW45, hWW45, WWP4) (PE)

Gene Names

SAV1; SAV; WW45; WWP4

Reactivity

Human

Applications

EIA, IF, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (FOXA1 monoclonal antibody (M03), clone 4F6 Western Blot analysis of FOXA1 expression in A-431 (Cat # L015V1).)

WB (Western Blot) (FOXA1 monoclonal antibody (M03), clone 4F6 Western Blot analysis of FOXA1 expression in HepG2 (Cat # L019V1).)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA1 on A-431 cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA1 on A-431 cell. [antibody concentration 10 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FOXA1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to FOXA1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml])

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FOXA1, Monoclonal Antibody (Cat# AAA26356)

Full Name

FOXA1 (Forkhead Box A1, HNF3A, MGC33105, TCF3A) (FITC)

Gene Names

FOXA1; HNF3A; TCF3A

Applications

IF, IHC, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PRKDC on HeLa cell. [antibody concentration 40 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PRKDC on HeLa cell. [antibody concentration 40 ug/ml])

Application Data (Detection limit for recombinant GST tagged PRKDC is 1 ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PRKDC on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PRKDC on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml])

WB (Western Blot) (PRKDC monoclonal antibody (M03), clone 2A8 Western Blot analysis of PRKDC expression in Hela S3 NE (Cat # L013V3).)

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PRKDC, Monoclonal Antibody (Cat# AAA26355)

Full Name

PRKDC (Protein Kinase, DNA-Activated, Catalytic Polypeptide, DNA-PKcs, DNAPK, DNPK1, HYRC, HYRC1, XRCC7, p350) (FITC)

Gene Names

PRKDC; HYRC; p350; DNAPK; DNPK1; HYRC1; IMD26; XRCC7; DNAPKc; DNA-PKC; DNA-PKcs

Applications

IF, IHC, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (ABHD5 monoclonal antibody. Western Blot analysis of ABHD5 expression in Jurkat.)

Application Data (Detection limit for recombinant GST tagged ABHD5 is ~1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ABHD5 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of ABHD5 expression in transfected 293T cell line by ABHD5 monoclonal antibody. Lane 1: ABHD5 transfected lysate (39kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (ABHD5 monoclonal antibody. Western Blot analysis of ABHD5 expression in human liver.)

WB (Western Blot) (Western Blot detection against Immunogen (64.5kD).)

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ABHD5, Monoclonal Antibody (Cat# AAA25591)

Full Name

ABHD5 (1-acylglycerol-3-phosphate O-acyltransferase ABHD5, Abhydrolase Domain-containing Protein 5, Lipid Droplet-binding Protein CGI-58, CGI-58, MGC8731, NCIE2) (PE)

Gene Names

ABHD5; CDS; CGI58; IECN2; NCIE2

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IP (Immunoprecipitation) (Immunoprecipitation of SOX9 transfected lysate using SOX9 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with SOX9 rabbit polyclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SOX9 on HepG2 cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SOX9 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 0.7ug/ml].)

WB (Western Blot) (Western Blot analysis of SOX9 expression in transfected 293T cell line by SOX9 monoclonal antibody. Lane 1: SOX9 transfected lysate (56.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (SOX9 monoclonal antibody, Western Blot analysis of SOX9 expression in HepG2.)

WB (Western Blot) (Western Blot detection against Immunogen (38.21kD).)

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SOX9, Monoclonal Antibody (Cat# AAA25850)

Full Name

SOX9 (Transcription Factor SOX-9, SOX 9) (PE)

Gene Names

SOX9; CMD1; SRA1; CMPD1

Reactivity

Human

Applications

EIA, IF, IHC, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IHC (Immunohistochemistry) (Figure 7. IHC analysis of Cdk2 using anti-Cdk2 antibody (AAA19658).Cdk2 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Cdk2 Antibody (AAA19658) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of Cdk2 using anti-Cdk2 antibody (AAA19658).Cdk2 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Cdk2 Antibody (AAA19658) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of Cdk2 using anti-Cdk2 antibody (AAA19658).Cdk2 was detected in a paraffin-embedded section of human serous adenocarcinoma of ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Cdk2 Antibody (AAA19658) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of Cdk2 using anti-Cdk2 antibody (AAA19658).Cdk2 was detected in a paraffin-embedded section of human renal clear cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Cdk2 Antibody (AAA19658) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of Cdk2 using anti-Cdk2 antibody (AAA19658).Cdk2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Cdk2 Antibody (AAA19658) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of Cdk2 using anti-Cdk2 antibody (AAA19658).Cdk2 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinomas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Cdk2 Antibody (AAA19658) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of Cdk2 using anti-Cdk2 antibody (AAA19658).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Jurkat whole cell lysates,Lane 2: human HepG2 whole cell lysates,Lane 3: human U20S whole cell lysates,Lane 4: rat heart tissue lysates,Lane 5: rat L6 whole cell lysates,Lane 6: mouse heart tissue lysates,Lane 7: mouse C2C12 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Cdk2 antigen affinity purified monoclonal antibody (#AAA19658) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cdk2 at approximately 30 kDa. The expected band size for Cdk2 is at 30 kDa.)

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Cdk2, Monoclonal Antibody (Cat# AAA19658)

Full Name

Anti-Cdk2 Antibody Picoband (monoclonal, 6D5B5)

Gene Names

CDK2; p33(CDK2)

Reactivity

Human, Mouse, Rat

Applications

IHC, WB

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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WB (Western Blot) (ACP1 monoclonal antibody Western Blot analysis of ACP1 expression in NIH/3T3.)

WB (Western Blot) (ACP1 monoclonal antibody Western Blot analysis of ACP1 expression in PC-12.)

Application Data (Detection limit for recombinant GST tagged ACP1 is ~3ng/ml as a capture antibody.)

WB (Western Blot) (Western Blot analysis of ACP1 expression in transfected 293T cell line by ACP1 monoclonal antibody. Lane 1: ACP1 transfected lysate (18kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (ACP1 monoclonal antibody Western Blot analysis of ACP1 expression in Raw 264.7.)

WB (Western Blot) (ACP1 monoclonal antibody Western Blot analysis of ACP1 expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (43.12kD).)

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ACP1, Monoclonal Antibody (Cat# AAA25006)

Full Name

ACP1 (Low Molecular Weight Phosphotyrosine Protein Phosphatase, LMW-PTP, LMW-PTPase, Low Molecular Weight Cytosolic Acid Phosphatase, Red Cell Acid Phosphatase 1, PTPase, Adipocyte Acid Phosphatase) (FITC)

Gene Names

ACP1; HAAP; LMW-PTP

Reactivity

Human, Mouse, Rat

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged BMP7 is 10 ng/ml as a capture antibody.)

WB (Western Blot) (Western Blot analysis of BMP7 expression in transfected 293T cell line by BMP7 monoclonal antibody (M03), clone S52.Lane 1: BMP7 transfected lysate(49.313 KDa).Lane 2: Non-transfected lysate.)

WB (Western Blot) (BMP7 monoclonal antibody (M03), clone S52. Western Blot analysis of BMP7 expression in A-549.)

WB (Western Blot) (BMP7 monoclonal antibody (M03), clone S52. Western Blot analysis of BMP7 expression in C32.)

WB (Western Blot) (BMP7 monoclonal antibody (M03), clone S52. Western Blot analysis of BMP7 expression in Raw 264.7.)

WB (Western Blot) (BMP7 monoclonal antibody (M03), clone S52. Western Blot analysis of BMP7 expression in human ovarian cancer.)

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BMP7, Monoclonal Antibody (Cat# AAA26052)

Full Name

BMP7 (Bone Morphogenetic Protein 7, OP-1) (APC)

Gene Names

BMP7; OP-1

Applications

WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Staining of human peripheral blood monocytes with MOUSE ANTI HUMAN CD274:RPE)

Application Data (Staining of human peripheral blood monocytes with C2549-22E4.)

Application Data (Staining of human peripheral blood monocytes with C2549-22E1.)

Application Data (Staining of human peripheral blood monocytes with C2549-22E1.)

Application Data (Jurkat cells were stained with C2549-22E (filled histogram) or isotype control (open histogram) followed by PE-conjugated anti-mouse antibody.)

Application Data (Staining of human peripheral blood monocytes with C2549-22E.)

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CD274, Monoclonal Antibody (Cat# AAA26820)

Full Name

CD274 (CD274 Antigen, B7 Homolog 1, B7H1, B7-H1, B7-H, MGC142294, MGC142296, OTTHUMP00000021029, Programmed Cell Death 1 Ligand 1, PDCD1 Ligand 1, PDCD1L1, PDCD1LG1, Programmed Death Ligand 1, PDL1, PD-L1, RGD1566211) (MaxLight 650)

Gene Names

CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1

Reactivity

Human

Applications

FC/FACS

Purity

Purified by protein G affinity chromatography from tissue culture supernatant.

Pricing

$830/0.1 mL $1,590/2x0.1 mL $2,305/3x0.1 mL $2,980/4x0.1 mL $3,425/5x0.1 mL

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WB (Western Blot) (CDC2 monoclonal antibody, Western Blot analysis of CDC2 expression in Hela.)

Application Data (Detection limit for recombinant GST tagged CDC2 is ~30ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CDC2 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CDC2 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 0.3ug/ml].)

WB (Western Blot) (Western Blot analysis of CDC2 expression in transfected 293T cell line by CDC2 monoclonal antibody. Lane 1: CDC2 transfected lysate (34.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (58.41kD).)

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CDC2, Monoclonal Antibody (Cat# AAA24458)

Full Name

CDC2 (Cell Division Control Protein 2 Homolog, CDC28A, Cell Division Protein Kinase 1, Cyclin-dependent Kinase 1, CDK1, p34 Protein Kinase, P34CDC2) APC

Gene Names

CDK1; CDC2; CDC28A; P34CDC2

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (DLX5 monoclonal antibody. Western Blot analysis of DLX5 expression in NIH/3T3.)

WB (Western Blot) (Western Blot analysis of DLX5 expression in transfected 293T cell line by DLX5 monoclonal antibody (M12). Lane 1: DLX5 transfected lysate (31.5kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (DLX5 monoclonal antibody, Western Blot analysis of DLX5 expression in A-431.)

WB (Western Blot) (DLX5 monoclonal antibody. Western Blot analysis of DLX5 expression in Raw 264.7.)

WB (Western Blot) (DLX5 monoclonal antibody. Western Blot analysis of DLX5 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (35.79kD).)

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DLX5, Monoclonal Antibody (Cat# AAA24782)

Full Name

DLX5 (Homeobox Protein DLX-5) (Biotin)

Gene Names

DLX5; SHFM1D

Reactivity

Human, Mouse, Rat

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western blot analysis of FOXA1 over-expressed 293 cell line, cotransfected with FOXA1 Validated Chimera RNAi ((Lane 2) or non-transfected control (Lane 1). Blot probed with FOXA1 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged FOXA1 is ~0.03ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FOXA1 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of FOXA1 expression in transfected 293T cell line by FOXA1 monoclonal antibody Lane 1: FOXA1 transfected lysate (49.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (FOXA1 monoclonal antibody Western Blot analysis of FOXA1 expression in HepG2)

WB (Western Blot) (Western Blot detection against Immunogen (37.4kD).)

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FOXA1, Monoclonal Antibody (Cat# AAA24806)

Full Name

FOXA1 (Forkhead Box A1, Hepatocyte Nuclear Factor 3 alpha, HNF-3-alpha, HNF3A, HNF-3A, MGC33105, Transcription Factor 3A, TCF3A, TCF-3A) (Biotin)

Gene Names

FOXA1; HNF3A; TCF3A

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (ACP1 monoclonal antibody Western Blot analysis of ACP1 expression in NIH/3T3.)

WB (Western Blot) (ACP1 monoclonal antibody Western Blot analysis of ACP1 expression in PC-12.)

Application Data (Detection limit for recombinant GST tagged ACP1 is ~3ng/ml as a capture antibody.)

WB (Western Blot) (Western Blot analysis of ACP1 expression in transfected 293T cell line by ACP1 monoclonal antibody. Lane 1: ACP1 transfected lysate (18kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (ACP1 monoclonal antibody Western Blot analysis of ACP1 expression in Raw 264.7.)

WB (Western Blot) (ACP1 monoclonal antibody Western Blot analysis of ACP1 expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (43.12kD).)

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ACP1, Monoclonal Antibody (Cat# AAA25595)

Full Name

ACP1 (Low Molecular Weight Phosphotyrosine Protein Phosphatase, LMW-PTP, LMW-PTPase, Low Molecular Weight Cytosolic Acid Phosphatase, Red Cell Acid Phosphatase 1, PTPase, Adipocyte Acid Phosphatase) (PE)

Gene Names

ACP1; HAAP; LMW-PTP

Reactivity

Human, Mouse, Rat

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IF (Immunofluorescence) (Figure 9. IF analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (AAA19660).CD147/Emmprin was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 ug/mL mouse anti-CD147/Emmprin Antibody (AAA19660) overnight at 4 degree C. Biotin conjugated goat anti-mouse IgG (BA1001) was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using DyLight488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 8. IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (AAA19660).CD147/Emmprin was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-CD147/Emmprin Antibody (AAA19660) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (AAA19660).CD147/Emmprin was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-CD147/Emmprin Antibody (AAA19660) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (AAA19660).CD147/Emmprin was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-CD147/Emmprin Antibody (AAA19660) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (AAA19660).CD147/Emmprin was detected in a paraffin-embedded section of human breast infiltrating ductal carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-CD147/Emmprin Antibody (AAA19660) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (AAA19660).CD147/Emmprin was detected in a paraffin-embedded section of human ahepatocellular carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-CD147/Emmprin Antibody (AAA19660) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (AAA19660).CD147/Emmprin was detected in a paraffin-embedded section of human laryngeal squamous cell carcinomas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-CD147/Emmprin Antibody (AAA19660) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (AAA19660).CD147/Emmprin was detected in a paraffin-embedded section of human bladder epithelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-CD147/Emmprin Antibody (AAA19660) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (AAA19660).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human HepG2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CD147/Emmprin antigen affinity purified monoclonal antibody (#AAA19660) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD147/Emmprin at approximately 35-60 kDa. The expected band size for CD147/Emmprin is at 42 kDa.)

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CD147/Emmprin, Monoclonal Antibody (Cat# AAA19660)

Full Name

Anti-CD147/Emmprin Antibody Picoband (monoclonal, 3B13G7)

Gene Names

BSG; M6; OK; 5F7; TCSF; CD147; EMMPRIN

Reactivity

Human, Mouse

Applications

WB, IHC-P, IF, ICC

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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WB (Western Blot) (Western Blot Analysis of human liver tissue lysate using Prohibitin Mouse Monoclonal Antibody (PHB/3194).)

Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using Monospecific Mouse Monoclonal Antibody to Prohibitin (PHB/3194). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

IF (Immunofluorescence) (Confocal Immunofluorescence of HepG2 cells stained with Prohibitin Mouse Monoclonal Antibody (PHB/3194) labeled with CF488 (Green); Reddot is used to label the nuclei.)

SDS-PAGE (SDS-PAGE Analysis Purified Prohibitin Mouse Monoclonal Antibody (PHB/3194). Confirmation of Purity and Integrity of Antibody.)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Liver stained with Prohibitin Mouse Monoclonal Antibody (PHB/3194).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Liver stained with Prohibitin Mouse Monoclonal Antibody (PHB/3194).)

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Prohibitin, Monoclonal Antibody (Cat# AAA23932)

Full Name

Prohibitin (Mitochondrial Marker)

Gene Names

PHB; PHB1; HEL-215; HEL-S-54e

Reactivity

Human

Applications

WB, IF, IHC

Purity

Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Pricing

$295/0.02 mg (With BSA & Azide at 0.2mg/mL) $475/0.1 mg (With BSA & Azide at 0.2mg/mL) $475/0.1 mg (Without BSA & Azide at 1mg/mL) $905/2x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,310/3x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,690/4x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,900/5x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,900/5x0.1 mg (Without BSA & Azide at 1mg/mL)

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WB (Western Blot) (Western blot analysis of CITED1 over-expressed 293 cell line, cotransfected with CITED1 Validated Chimera RNAi ((Lane 2) or non-transfected control (Lane 1). Blot probed with CITED1 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged CITED1 is ~0.3ng/ml as a capture antibody.)

WB (Western Blot) (Western Blot analysis of CITED1 expression in transfected 293T cell line by CITED1 monoclonal antibody. Lane 1: CITED1 transfected lysate (19.9kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (CITED1 monoclonal antibody Western Blot analysis of CITED1 expression in A-431.)

WB (Western Blot) (CITED1 monoclonal antibody. Western Blot analysis of CITED1 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

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CITED1, Monoclonal Antibody (Cat# AAA24466)

Full Name

CITED1 (CBP/p300 Interacting Transactivator 1, Cbp/p300-interacting Transactivator with Glu/Asp-rich Carboxy-terminal Domain 1, Melanocyte-specific Protein1) APC

Gene Names

CITED1; MSG1

Reactivity

Human, Rat

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (ACP1 monoclonal antibody Western Blot analysis of ACP1 expression in NIH/3T3.)

WB (Western Blot) (ACP1 monoclonal antibody Western Blot analysis of ACP1 expression in PC-12.)

Application Data (Detection limit for recombinant GST tagged ACP1 is ~3ng/ml as a capture antibody.)

WB (Western Blot) (Western Blot analysis of ACP1 expression in transfected 293T cell line by ACP1 monoclonal antibody. Lane 1: ACP1 transfected lysate (18kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (ACP1 monoclonal antibody Western Blot analysis of ACP1 expression in Raw 264.7.)

WB (Western Blot) (ACP1 monoclonal antibody Western Blot analysis of ACP1 expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (43.12kD).)

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ACP1, Monoclonal Antibody (Cat# AAA25302)

Full Name

ACP1 (Low Molecular Weight Phosphotyrosine Protein Phosphatase, LMW-PTP, LMW-PTPase, Low Molecular Weight Cytosolic Acid Phosphatase, Red Cell Acid Phosphatase 1, PTPase, Adipocyte Acid Phosphatase) (HRP)

Gene Names

ACP1; HAAP; LMW-PTP

Reactivity

Human, Mouse, Rat

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (STK33 monoclonal antibody (M09), clone 1F10. Western Blot analysis of STK33 expression in Raw 264.7.)

WB (Western Blot) (STK33 monoclonal antibody (M09), clone 1F10. Western Blot analysis of STK33 expression in PC-12.)

WB (Western Blot) (STK33 monoclonal antibody (M09), clone 1F10. Western Blot analysis of STK33 expression in NIH/3T3.)

WB (Western Blot) (STK33 monoclonal antibody (M09), clone 1F10 Western Blot analysis of STK33 expression in A-431 (Cat # L015V1).)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to STK33 on A-431 cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to STK33 on A-431 cell. [antibody concentration 10 ug/ml])

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STK33, Monoclonal Antibody (Cat# AAA26587)

Full Name

STK33 (Serine/Threonine Kinase 33) (PE)

Applications

EIA, IF, IP, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

What are Monoclonal Antibodies?

Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.

This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.

AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.

What Are The Uses of Monoclonal Antibodies

Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.

ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.

Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.

Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.

Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.

How Monoclonal Antibodies Are Used as Medicine?

Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).

Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:

• Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.


• Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.


• Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.

Why Buy Monoclonal Antibodies From Us?

At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.

1. Validated for Versatile Applications

The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).

2. Wide Selection & Specialized Options

We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.

3. High-Quality Proteins

Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.

Frequently Asked Questions

1. Are your monoclonal antibodies validated for specific applications?

Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.

2. How do I choose the right monoclonal antibody for my application?

Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.

3. How quickly can I receive my order?

Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.