Monoclonal Antibodies

Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.

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WB (Western Blot) (GLMN monoclonal antibody Western Blot analysis of GLMN expression in HL-60.)

WB (Western Blot) (GLMN monoclonal antibody Western Blot analysis of GLMN expression in Jurkat.)

Application Data (Detection limit for recombinant GST tagged GLMN is ~0.03ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of GLMN transfected lysate using GLMN monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GLMN rabbit polyclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GLMN on HeLa cell. [antibody concentration 20ug/ml])

WB (Western Blot) (Western Blot analysis of GLMN expression in transfected 293T cell line by GLMN monoclonal antibody. Lane 1: GLMN transfected lysate (68.2kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (91.45kD).)

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GLMN, Monoclonal Antibody (Cat# AAA24225)

Full Name

GLMN (FAP48, FAP68, VMGLOM, Glomulin, FK506-binding Protein-associated Protein, FAP, FKBP-associated Protein) (AP)

Gene Names

GLMN; FAP; GVM; GLML; FAP48; FAP68; FKBPAP; VMGLOM

Reactivity

Human

Applications

EIA, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (SMAD3 monoclonal antibody (M01), clone 2C12. Western Blot analysis of SMAD3 expression in Hs 181.Tes.)

WB (Western Blot) (SMAD3 monoclonal antibody (M01), clone 2C12. Western Blot analysis of SMAD3 expression in HeLa.)

WB (Western Blot) (Western Blot analysis of SMAD3 expression in transfected 293T cell line by SMAD3 monoclonal antibody (M01), clone 2C12.Lane 1: SMAD3 transfected lysate(48 KDa).Lane 2: Non-transfected lysate.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SMAD3 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 6 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SMAD3 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 6 ug/ml])

WB (Western Blot) (SMAD3 monoclonal antibody (M01), clone 2C12. Western Blot analysis of SMAD3 expression in human colon.)

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SMAD3, Monoclonal Antibody (Cat# AAA26030)

Full Name

SMAD3 (SMAD Family Member 3, DKFZp586N0721, DKFZp686J10186, HSPC193, HsT17436, JV15-2, MADH3, MGC60396) (APC)

Gene Names

SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436

Applications

IHC, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (FLJ20097 monoclonal antibody, Western Blot analysis of FLJ20097 expression in HeLa.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FLJ20097 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FLJ20097 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml].)

WB (Western Blot) (FLJ20097 monoclonal antibody. Western Blot analysis of FLJ20097 expression in NIH/3T3.)

WB (Western Blot) (FLJ20097 monoclonal antibody. Western Blot analysis of FLJ20097 expression in human pancreas.)

WB (Western Blot) (Western Blot detection against Immunogen (37.44kD).)

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CCDC132, Monoclonal Antibody (Cat# AAA24509)

Full Name

CCDC132 (Coiled-coil Domain-containing Protein 132, KIAA1861, DKFZp313I2429, FLJ20097, FLJ23581, KIAA1861, MGC176659) APC

Reactivity

Human, Mouse

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (LRRN1 monoclonal antibody (M05), clone 3D11. Western Blot analysis of LRRN1 expression in PC-12.)

WB (Western Blot) (LRRN1 monoclonal antibody (M05), clone 3D11. Western Blot analysis of LRRN1 expression in Raw 264.7.)

Application Data (Detection limit for recombinant GST tagged LRRN1 is 3 ng/ml as a capture antibody.)

WB (Western Blot) (LRRN1 monoclonal antibody (M05), clone 3D11. Western Blot analysis of LRRN1 expression in HeLa.)

WB (Western Blot) (Western Blot analysis of LRRN1 expression in transfected 293T cell line by LRRN1 monoclonal antibody (M05), clone 3D11.Lane 1: LRRN1 transfected lysate (Predicted MW: 78.76 KDa).Lane 2: Non-transfected lysate.)

Application Data (Detection limit for recombinant GST tagged LRRN1 is 0.3 ng/ml as a capture antibody.)

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LRRN1, Monoclonal Antibody (Cat# AAA26018)

Full Name

LRRN1 (Leucine Rich Repeat Neuronal 1, FIGLER3, KIAA1497, NLRR-1, LRCH4) (AP)

Reactivity

Human, Mouse, Rat

Applications

WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (SMAD3 monoclonal antibody. Western Blot analysis of SMAD3 expression in human colon.)

WB (Western Blot) (SMAD3 monoclonal antibody. Western Blot analysis of SMAD3 expression in HeLa.)

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between MAPK8 and SMAD3. HeLa cells were stained with MAPK8 rabbit purified polyclonal 1:1200 and 133539 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Application Data (Detection limit for recombinant GST tagged SMAD3 is ~0.1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of 133539 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of SMAD3 expression in transfected 293T cell line by 133539. Lane 1: SMAD3 transfected lysate (48kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot analysis of SMAD3 expression in PC-12 using 133539.)

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SMAD3, Monoclonal Antibody (Cat# AAA25544)

Full Name

SMAD3 (Mothers Against Decapentaplegic Homolog 3, SMAD 3, Mothers Against DPP Homolog 3, MAD Homolog 3, Mad3, hMAD-3, SMAD Family Member 3, JV15-2, hSMAD3, MADH3, DKFZp586N0721, DKFZp686J10186, HSPC193, HsT17436, MGC60396) (HRP)

Gene Names

SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436

Reactivity

Human, Rat

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (CAPNS1 monoclonal antibody, Western Blot analysis of CAPNS1 expression in A-431.)

WB (Western Blot) (Western blot analysis of CAPNS1 over-expressed 293 cell line, cotransfected with CAPNS1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CAPNS1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged CAPNS1 is ~0.1ng/ml when antibody 124328 was used as capture antibody.)

IF (Immunofluorescence) (Immunofluorescence staining of CAPNS1 on HeLa cells using antibody 124328 at 20ug/ml.)

IHC (Immunohistochemistry) (Immunoperoxidase staining of CAPNS1 on formalin-fixed paraffin-embedded human kidney using antibody 124328 at 3ug/ml.)

WB (Western Blot) (Western Blot analysis of CAPNS1 expression in transfected 293T cell line using antibody 124328: Lane 1: CAPNS1 transfected lysate (28.3kD) Lane 2: Non-transfected lysate)

WB (Western Blot) (Western Blot analysis of CAPNS1 expression in PC-12 cells using antibody 124328.)

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CAPNS1, Monoclonal Antibody (Cat# AAA25043)

Full Name

CAPNS1 (Calpain Small Subunit 1, CSS1, Calcium-activated Neutral Proteinase Small Subunit, CANP Small Subunit, Calcium-dependent Protease Small Subunit, CDPS, Calcium-dependent Protease Small Subunit 1, Calpain Regulatory Subunit, CAPN4, CAPNS) (FITC)

Gene Names

CAPNS1; CANP; CDPS; CSS1; CANPS; CAPN4; CALPAIN4

Reactivity

Human, Rat

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged BMP7 is 10 ng/ml as a capture antibody.)

WB (Western Blot) (Western Blot analysis of BMP7 expression in transfected 293T cell line by BMP7 monoclonal antibody (M03), clone S52.Lane 1: BMP7 transfected lysate (49.313 KDa).Lane 2: Non-transfected lysate.)

WB (Western Blot) (BMP7 monoclonal antibody (M03), clone S52. Western Blot analysis of BMP7 expression in A-549 (Cat # L025V1).)

WB (Western Blot) (BMP7 monoclonal antibody (M03), clone S52. Western Blot analysis of BMP7 expression in C32 (Cat # L002V1).)

WB (Western Blot) (BMP7 monoclonal antibody (M03), clone S52. Western Blot analysis of BMP7 expression in Raw 264.7.)

WB (Western Blot) (BMP7 monoclonal antibody (M03), clone S52. Western Blot analysis of BMP7 expression in human ovarian cancer.)

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BMP7, Monoclonal Antibody (Cat# AAA26318)

Full Name

BMP7 (Bone Morphogenetic Protein 7, OP-1) (FITC)

Gene Names

BMP7; OP-1

Applications

WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (AKT1 monoclonal antibody (M08), clone 1D4. Western Blot analysis of AKT1 expression in Raw 264.7.)

WB (Western Blot) (AKT1 monoclonal antibody (M08), clone 1D4. Western Blot analysis of AKT1 expression in NIH/3T3.)

Application Data (Detection limit for recombinant GST tagged AKT1 is approximately 1ng/ml as a capture antibody.)

WB (Western Blot) (AKT1 monoclonal antibody (M08), clone 1D4 Western Blot analysis of AKT1 expression in Jurkat (Cat # L017V1).)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to AKT1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to AKT1 on HeLa cell. [antibody concentration 10 ug/ml])

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AKT1, Monoclonal Antibody (Cat# AAA26583)

Full Name

AKT1 (v-akt murine Thymoma Viral Oncogene Homolog 1, AKT, MGC99656, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA) (PE)

Gene Names

AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Applications

IF, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (CRYM monoclonal antibody, Western Blot analysis of CRYM expression in Jurkat.)

Application Data (Detection limit for recombinant GST tagged CRYM is ~0.03ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of CRYM transfected lysate using CRYM monoclonal antibody and Protein A Magnetic Bead and immunoblotted with CRYM rabbit polyclonal antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CRYM on formalin-fixed paraffin-embedded human skeletal muscle. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of CRYM expression in transfected 293T cell line by CRYM monoclonal antibody. Lane 1: CRYM transfected lysate (33.8kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

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CRYM, Monoclonal Antibody (Cat# AAA25065)

Full Name

CRYM (Thiomorpholine-carboxylate Dehydrogenase, Mu-crystallin Homolog, NADP-regulated Thyroid-hormone-binding Protein, Ketimine Reductase, THBP) (FITC)

Gene Names

CRYM; THBP; DFNA40

Reactivity

Human

Applications

EIA, IHC, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IF (Immunofluorescence) (Figure 8. IF analysis of eRF1/ETF1 using anti- eRF1/ETF1 antibody (AAA19383).eRF1/ETF1 was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti- eRF1/ETF1 Antibody (AAA19383) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of eRF1/ETF1 using anti-eRF1/ETF1 antibody (AAA19383).eRF1/ETF1 was detected in paraffin-embedded section of rat pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-eRF1/ETF1 Antibody (AAA19383) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of eRF1/ETF1 using anti-eRF1/ETF1 antibody (AAA19383).eRF1/ETF1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-eRF1/ETF1 Antibody (AAA19383) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of eRF1/ETF1 using anti-eRF1/ETF1 antibody (AAA19383).eRF1/ETF1 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-eRF1/ETF1 Antibody (AAA19383) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of eRF1/ETF1 using anti-eRF1/ETF1 antibody (AAA19383).eRF1/ETF1 was detected in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-eRF1/ETF1 Antibody (AAA19383) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of eRF1/ETF1 using anti-eRF1/ETF1 antibody (AAA19383).eRF1/ETF1 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-eRF1/ETF1 Antibody (AAA19383) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

WB (Western Blot) (Figure 2. Western blot analysis of eRF1/ETF1 using anti-eRF1/ETF1 antibody (AAA19383).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat liver tissue lysatesLane 2: rat testis tissue lysatesLane 3: rat kidney tissue lysatesLane 4: rat PC-12 whole cell lysatesLane 5: mouse liver tissue lysatesLane 6: mouse testis tissue lysatesLane 7: mouse kidney tissue lysatesLane 8: mouse RAW264. 7 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti-eRF1/ETF1 antigen affinity purified monoclonal antibody (Catalog # AAA19383) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for eRF1/ETF1 at approximately 49KD. The expected band size for eRF1/ETF1 is at 49KD.)

WB (Western Blot) (Figure 1. Western blot analysis of eRF1/ETF1 using anti-eRF1/ETF1 antibody (AAA19383).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hela whole cell lysatesLane 2: human Jurkat whole cell lysatesLane 3: human K562 whole cell lysatesLane 4: human Raji whole cell lysatesLane 5: human HEPG2 whole cell lysatesLane 6: human HEPG2 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti-eRF1/ETF1 antigen affinity purified monoclonal antibody (Catalog # AAA19383) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for eRF1/ETF1 at approximately 49KD. The expected band size for eRF1/ETF1 is at 49KD.)

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eRF1/ETF1, Monoclonal Antibody (Cat# AAA19383)

Full Name

Anti-eRF1/ETF1 Antibody (monoclonal, 3E5)

Gene Names

ETF1; ERF; RF1; ERF1; TB3-1; D5S1995; SUP45L1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, ICC, IF, FC/FACS/FCM

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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IHC (Immunohistchemistry) (Figure 9. IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (AAA19701).PC4/SUB1 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-PC4/SUB1 Antibody (AAA19701) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 8. IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (AAA19701).PC4/SUB1 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-PC4/SUB1 Antibody (AAA19701) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (AAA19701).PC4/SUB1 was detected in a paraffin-embedded section of human renal cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-PC4/SUB1 Antibody (AAA19701) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (AAA19701).PC4/SUB1 was detected in a paraffin-embedded section of human poison impregnated thyroid gland tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-PC4/SUB1 Antibody (AAA19701) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (AAA19701).PC4/SUB1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-PC4/SUB1 Antibody (AAA19701) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (AAA19701).PC4/SUB1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-PC4/SUB1 Antibody (AAA19701) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (AAA19701).PC4/SUB1 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-PC4/SUB1 Antibody (AAA19701) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (AAA19701).PC4/SUB1 was detected in a paraffin-embedded section of human colonic adenoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-PC4/SUB1 Antibody (AAA19701) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (AAA19701).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human Jurkat whole cell lysates,Lane 3: human A549 whole cell lysates,Lane 4: human HepG2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PC4/SUB1 antigen affinity purified monoclonal antibody (#AAA19701) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PC4/SUB1 at approximately 18 kDa. The expected band size for PC4/SUB1 is at 18 kDa.)

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PC4/SUB1, Monoclonal Antibody (Cat# AAA19701)

Full Name

Anti-PC4/SUB1 Antibody Picoband (monoclonal, 6B5B10)

Gene Names

SUB1; P15; PC4; p14

Reactivity

Human, Mouse, Rat

Applications

WB, IHC

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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Application Data (Detection limit for recombinant GST tagged UPB1 is 1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to UPB1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of UPB1 expression in transfected 293T cell line by UPB1 monoclonal antibody. Lane 1: UPB1 transfected lysate (43.2kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (UPB1 monoclonal antibody. Western Blot analysis of UPB1 expression in Jurkat.)

WB (Western Blot) (UPB1 monoclonal antibody. Western Blot analysis of UPB1 expression in human liver.)

WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)

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UPB1, Monoclonal Antibody (Cat# AAA24994)

Full Name

UPB1 (Beta-ureidopropionase, BUP1, BUP-1, Beta-alanine Synthase, N-carbamoyl-beta-alanine Amidohydrolase) (Biotin)

Gene Names

UPB1; BUP1

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SP1 on HeLa cell. [antibody concentration 20 ug/ml])

WB (Western Blot) (SP1 monoclonal antibody (M03), clone 4C8 Western Blot analysis of SP1 expression in Hela S3 NE.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human smooth muscle. [antibody concentration 3 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human smooth muscle. [antibody concentration 3 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml])

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SP1, Monoclonal Antibody (Cat# AAA26036)

Full Name

SP1 (Sp1 Transcription Factor) (APC)

Applications

IF, IHC, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (CLK3 monoclonal antibody. Western Blot analysis of CLK3 expression in Hela NE.)

WB (Western Blot) (Western blot analysis of CLK3 over-expressed 293 cell line, cotransfected with CLK3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CLK3 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CLK3 on HeLa cell. [antibody concentration 8ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CLK3 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.5ug/ml].)

WB (Western Blot) (Western Blot analysis of CLK3 expression in transfected 293T cell line by CLK3 monoclonal antibody. Lane 1: CLK3 transfected lysate (58.6kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

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CLK3, Monoclonal Antibody (Cat# AAA24467)

Full Name

CLK3 (Dual Specificity Protein Kinase CLK3, CDC-like Kinase 3) APC

Gene Names

CLK3; PHCLK3; PHCLK3/152

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data

Application Data

Application Data

Application Data

Application Data

IHC (Immunohistochemistry) (Immunohistochemistry (FFPE) analysis of human tonsil stained with C2399-07G2, biotinylated anti-mouse IgG and Streptavidin Alkaline Phosphatase (red). Nuclei were counterstained with hematoxylin.)

IHC (Immunohistochemistry) (immunohistochemical analysis of an acetone fixed, frozen human colon section using C2399-07G2.)

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CD45, Monoclonal Antibody (Cat# AAA26824)

Full Name

CD45 (CD45 Antigen, B220, GP180, Leukocyte Common Antigen, LCA, L-CA, LY5, LY-5, Protein Tyrosine Phosphatase Receptor Type C, Protein Tyrosine Phosphatase Receptor Type C Polypeptide, PTPRC, T200, T200 Glycoprotein) (MaxLight 650)

Gene Names

PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180

Reactivity

Equine, Human

Applications

FC/FACS, IHC, IP

Purity

Purified by Protein A Affinity Chromatography

Pricing

$1,010/0.1 mL $1,950/2x0.1 mL $2,835/3x0.1 mL $3,675/4x0.1 mL $4,260/5x0.1 mL

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WB (Western Blot) (ASB9 monoclonal antibody, Western Blot analysis of ASB9 expression in HepG2.)

Application Data (Detection limit for recombinant GST tagged ASB9 is ~0.1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of ASB9 transfected lysate using ASB9 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with ASB9 rabbit polyclonal antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ASB9 on formalin-fixed paraffin-embedded human lymphoma tissue. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of ASB9 expression in transfected 293T cell line by ASB9 monoclonal antibody. Lane 1: ASB9 transfected lysate (31.9kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (53.83kD).)

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ASB9, Monoclonal Antibody (Cat# AAA25029)

Full Name

ASB9 (Ankyrin Repeat and SOCS Box Protein 9, ASB-9, DKFZp564L0862, FLJ20636, MGC4954) (FITC)

Reactivity

Human

Applications

EIA, IHC, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (ABHD5 monoclonal antibody. Western Blot analysis of ABHD5 expression in Jurkat.)

Application Data (Detection limit for recombinant GST tagged ABHD5 is ~1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ABHD5 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of ABHD5 expression in transfected 293T cell line by ABHD5 monoclonal antibody. Lane 1: ABHD5 transfected lysate (39kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (ABHD5 monoclonal antibody. Western Blot analysis of ABHD5 expression in human liver.)

WB (Western Blot) (Western Blot detection against Immunogen (64.5kD).)

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ABHD5, Monoclonal Antibody (Cat# AAA25298)

Full Name

ABHD5 (1-acylglycerol-3-phosphate O-acyltransferase ABHD5, Abhydrolase Domain-containing Protein 5, Lipid Droplet-binding Protein CGI-58, CGI-58, MGC8731, NCIE2) (HRP)

Gene Names

ABHD5; CDS; CGI58; IECN2; NCIE2

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (HSPA1L monoclonal antibody Western Blot analysis of HSPA1L expression in PC-12.)

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between MAP3K7 and HSPA1L HeLa cells were stained with MAP3K7 rabbit purified polyclonal 1:1200 and HSPA1L mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Application Data (Detection limit for recombinant GST tagged HSPA1L is ~0.1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HSPA1L on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml])

WB (Western Blot) (HSPA1L monoclonal antibody Western Blot analysis of HSPA1L expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (34.65kD).)

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HSPA1L, Monoclonal Antibody (Cat# AAA24538)

Full Name

HSPA1L (Heat Shock 70kD Protein 1-like, HSP70-Hom, Heat shock 70kD protein 1L, Heat Shock 70kD Protein 1-Hom) APC

Gene Names

HSPA1L; HSP70T; hum70t; HSP70-1L; HSP70-HOM

Reactivity

Human, Rat

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged RPL36A is ~1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of AAA24050 (10ug/ml) to RPL36A on HeLa cells.)

WB (Western Blot) (Western Blot analysis of RPL36A expression in NIH/3T3 using AAA24050.)

Application Data

WB (Western Blot) (Western Blot analysis of RPL36A expression in PC-12 using AAA24050.)

WB (Western Blot) (Western Blot analysis of RPL36A expression in HeLa using AAA24050.)

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RPL36A, Monoclonal Antibody (Cat# AAA24050)

Full Name

RPL36A (60S Ribosomal Protein L36a, 60S Ribosomal Protein L44, Cell Migration-inducing Gene 6 Protein, Cell Growth-inhibiting Gene 15 Protein, RPL44, GIG15, MIG6, MGC72020)

Gene Names

RPL36A; L36A; L44L; MIG6; RPL44

Reactivity

Species Crossreactivity: mouse and rat.

Applications

EL/EIA, WB, IF

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

$820/0.1 mg $1,565/2x0.1 mg $2,270/3x0.1 mg $2,940/4x0.1 mg $3,380/5x0.1 mg

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IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to TAF11 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 1.2 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TAF11 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 1.2 ug/ml])

Application Data (Detection limit for recombinant GST tagged TAF11 is approximately 0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TAF11 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TAF11 on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (TAF11 monoclonal antibody (M06), clone 3G6 Western Blot analysis of TAF11 expression in Hela S3 NE (Cat # L013V3).)

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TAF11, Monoclonal Antibody (Cat# AAA26352)

Full Name

TAF11 (TAF11 RNA Polymerase II, TATA Box Binding Protein (TBP)-Associated Factor, 28kD, MGC:15243, PRO2134, TAF2I, TAFII28) (FITC)

Gene Names

TAF11; TAF2I; PRO2134; TAFII28; MGC:15243

Applications

IF, IHC, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (HOXC12 monoclonal antibody Western Blot analysis of HOXC12 expression in NIH/3T3.)

Application Data (Detection limit for recombinant GST tagged HOXC12 is ~0.1ng/ml as a capture antibody.)

WB (Western Blot) (HOXC12 monoclonal antibody Western Blot analysis of HOXC12 expression in Jurkat.)

WB (Western Blot) (HOXC12 monoclonal antibody Western Blot analysis of HOXC12 expression in Raw 264.7.)

WB (Western Blot) (HOXC12 monoclonal antibody Western Blot analysis of HOXC12 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

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HOXC12, Monoclonal Antibody (Cat# AAA24831)

Full Name

HOXC12 (Homeobox Protein Hox-C12, Homeobox Protein Hox-3F, HOC3F, HOX3F) (Biotin)

Gene Names

HOXC12; HOX3; HOC3F; HOX3F

Reactivity

Human, Mouse, Rat

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western blot analysis of HNF4A over-expressed 293 cell line, cotransfected with HNF4A Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with HNF4A monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged HNF4A is ~1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HNF4A on HeLa cell. [antibody concentration 10ug/ml])

WB (Western Blot) (Western Blot analysis of HNF4A expression in transfected 293T cell line by HNF4A monoclonal antibody. Lane 1: HNF4A transfected lysate (51.6kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (HNF4A monoclonal antibody Western Blot analysis of HNF4A expression in Jurkat.)

WB (Western Blot) (HNF4A monoclonal antibody Western Blot analysis of HNF4A expression in HepG2)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

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HNF4A, Monoclonal Antibody (Cat# AAA24829)

Full Name

HNF4A (Hepatocyte Nuclear Factor 4-alpha, HNF-4-alpha, Transcription Factor HNF-4, Nuclear Receptor Subfamily 2 Group A Member 1, Transcription Factor 14, HNF4, NR2A1, TCF14) (Biotin)

Gene Names

HNF4A; TCF; HNF4; MODY; FRTS4; MODY1; NR2A1; TCF14; HNF4a7; HNF4a8; HNF4a9; NR2A21; HNF4alpha

Reactivity

Human

Applications

EIA, IF, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of A431 cells with Desmoglein 2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Desmoglein 2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Desmoglein 2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Desmoglein 2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Desmoglein 2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Desmoglein 2 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Desmoglein 2 on A431 cell using anti-Desmoglein 2 antibody at 1/500 dilution.)

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Desmoglein 2, Monoclonal Antibody (Cat# AAA30463)

Full Name

Desmoglein 2 Antibody

Gene Names

DSG2; HDGC; CDHF5; ARVC10; ARVD10; CMD1BB

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, FC/FACS

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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WB (Western Blot) (ABHD5 monoclonal antibody. Western Blot analysis of ABHD5 expression in Jurkat.)

Application Data (Detection limit for recombinant GST tagged ABHD5 is ~1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ABHD5 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of ABHD5 expression in transfected 293T cell line by ABHD5 monoclonal antibody. Lane 1: ABHD5 transfected lysate (39kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (ABHD5 monoclonal antibody. Western Blot analysis of ABHD5 expression in human liver.)

WB (Western Blot) (Western Blot detection against Immunogen (64.5kD).)

Compare (5 max)

ABHD5, Monoclonal Antibody (Cat# AAA25002)

Full Name

ABHD5 (1-acylglycerol-3-phosphate O-acyltransferase ABHD5, Abhydrolase Domain-containing Protein 5, Lipid Droplet-binding Protein CGI-58, CGI-58, MGC8731, NCIE2) (FITC)

Gene Names

ABHD5; CDS; CGI58; IECN2; NCIE2

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (LEPRE1 monoclonal antibody, Western Blot analysis of LEPRE1 expression in HeLa.)

Application Data (Detection limit for recombinant GST tagged LEPRE1 is ~0.3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to LEPRE1 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to LEPRE1 on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 6ug/ml].)

WB (Western Blot) (Western Blot analysis of LEPRE1 expression in transfected 293T cell line by LEPRE1 monoclonal antibody. Lane 1: LEPRE1 transfected lysate (46kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (37kD).)

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GROS1, Monoclonal Antibody (Cat# AAA24228)

Full Name

GROS1 (Growth Suppressor 1, Leucine- and Proline-enriched Proteoglycan 1, LEPRE1, Leprecan-1, MGC117314, OI8, Prolyl 3-hydroxylase 1, P3H1, PSEC0109) (AP)

Gene Names

P3H1; OI8; GROS1; LEPRE1

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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<< Previous Next >>

Application Data (Detection limit for recombinant GST tagged MECP2 is ~1ng/ml as a capture antibody.)

IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to MECP2 on formalin-fixed paraffin-embedded human heart. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of MECP2 expression in transfected 293T cell line by MECP2 monoclonal antibody. Lane 1: MECP2 transfected lysate (52.4kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (MECP2 monoclonal antibody. Western Blot analysis of MECP2 expression in NIH/3T3.)

WB (Western Blot) (MECP2 monoclonal antibody Western Blot analysis of MECP2 expression in MCF-7,)

WB (Western Blot) (MECP2 monoclonal antibody. Western Blot analysis of MECP2 expression in rat muscle.)

WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)

Compare (5 max)

MECP2, Monoclonal Antibody (Cat# AAA24270)

Full Name

MECP2 (Methyl-CpG-binding Protein 2, MeCp-2 Protein, MeCp2, DKFZp686A24160) (AP)

Gene Names

MECP2; RS; RTS; RTT; PPMX; MRX16; MRX79; MRXSL; AUTSX3; MRXS13

Reactivity

Human, Mouse, Rat

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Staining of human peripheral blood monocytes with MOUSE ANTI HUMAN CD274:RPE)

Application Data (Staining of human peripheral blood monocytes with C2549-22E4.)

Application Data (Staining of human peripheral blood monocytes with C2549-22E1.)

Application Data (Staining of human peripheral blood monocytes with C2549-22E1.)

Application Data (Jurkat cells were stained with C2549-22E (filled histogram) or isotype control (open histogram) followed by PE-conjugated anti-mouse antibody.)

Application Data (Staining of human peripheral blood monocytes with C2549-22E.)

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CD274, Monoclonal Antibody (Cat# AAA26834)

Full Name

CD274 (CD274 Antigen, B7 Homolog 1, B7H1, B7-H1, B7-H, MGC142294, MGC142296, OTTHUMP00000021029, Programmed Cell Death 1 Ligand 1, PDCD1 Ligand 1, PDCD1L1, PDCD1LG1, Programmed Death Ligand 1, PDL1, PD-L1, RGD1566211) (MaxLight 750)

Gene Names

CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1

Reactivity

Human

Applications

FC/FACS

Purity

Purified by protein G affinity chromatography from tissue culture supernatant.

Pricing

$830/0.1 mL $1,590/2x0.1 mL $2,305/3x0.1 mL $2,980/4x0.1 mL $3,425/5x0.1 mL

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WB (Western Blot) (HHIP monoclonal antibody. Western Blot analysis of HHIP expression in MCF-7.)

WB (Western Blot) (HHIP monoclonal antibody, Western Blot analysis of HHIP expression in Hela NE.)

Application Data (Detection limit for recombinant GST tagged HHIP is ~1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of HHIP transfected lysate using HHIP monoclonal antibody and Protein A Magnetic Bead and immunoblotted with HHIP rabbit polyclonal antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HHIP on formalin-fixed paraffin-embedded human heart. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of HHIP expression in transfected 293T cell line by HHIP monoclonal antibody. Lane 1: HHIP transfected lysate (78.9kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)

Compare (5 max)

HHIP, Monoclonal Antibody (Cat# AAA24532)

Full Name

HHIP (Hedgehog-interacting Protein, HHIP, HIP, UNQ5825/PRO19644, FLJ20992, FLJ90230) APC

Gene Names

HHIP; HIP

Reactivity

Human

Applications

EIA, IHC, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western Blot analysis of TUBA1 expression in transfected 293T cell line by TUBA1 monoclonal antibody. Lane 1: TUBA1 transfected lysate (49.9kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (TUBA1 monoclonal antibody. Western Blot analysis of TUBA1 expression in HeLa.)

WB (Western Blot) (Western blot analysis of TUBA1 over-expressed 293 cell line, cotransfected with TUBA1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TUBA1 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TUBA1 on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TUBA1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml])

WB (Western Blot) (TUBA1 monoclonal antibody. Western Blot analysis of TUBA1 expression in different Cell lines and Human Tissue.)

WB (Western Blot) (Western Blot detection against Immunogen (75.02kD).)

Compare (5 max)

Tubulin alpha-4A Chain, Monoclonal Antibody (Cat# AAA25577)

Full Name

Tubulin alpha-4A Chain (TUBA4A, Alpha-tubulin 1, Tubulin alpha-1 Chain, TUBA1, Testis-specific alpha-tubulin, Tubulin H2-alpha) (HRP)

Gene Names

TUBA4A; ALS22; TUBA1; H2-ALPHA

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (LIMK1 monoclonal antibody. Western Blot analysis of LIMK1 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

Application Data (Detection limit for recombinant GST tagged LIMK1 is ~0.03ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to LIMK1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3ug/ml])

WB (Western Blot) (LIMK1 monoclonal antibody. Western Blot analysis of LIMK1 expression in NIH/3T3.)

WB (Western Blot) (LIMK1 monoclonal antibody. Western Blot analysis of LIMK1 expression in Raw 264.7.)

WB (Western Blot) (LIMK1 monoclonal antibody Western Blot analysis of LIMK1 expression in SW-13.)

Compare (5 max)

LIMK1, Monoclonal Antibody (Cat# AAA24555)

Full Name

LIMK1 (LIM Domain Kinase 1, LIMK-1, LIMK) APC

Gene Names

LIMK1; LIMK; LIMK-1

Reactivity

Human, Mouse, Rat

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Dilution: IF: 1:50-200 WB 1:1000-2000, IHC 1:100-200)

Application Data (Dilution: IF: 1:50-200 WB 1:1000-2000, IHC 1:100-200)

Application Data (Dilution: IF: 1:50-200 WB 1:1000-2000, IHC 1:100-200)

Application Data (Dilution: IF: 1:50-200 WB 1:1000-2000, IHC 1:100-200)

Application Data (Dilution: IF: 1:50-200 WB 1:1000-2000, IHC 1:100-200)

IHC (Immunohistochemistry-Paraffin) (Dilution: IF: 1:50-200 WB 1:1000-2000, IHC 1:100-200)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 WB 1:1000-2000, IHC 1:100-200)

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Bax, Monoclonal Antibody (Cat# AAA28907)

Full Name

Bax mouse Monoclonal Antibody (6F11)

Gene Names

BAX; BCL2L4

Reactivity

Human, Mouse, Rat, Chicken

Applications

WB, IHC-P, IF

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

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Application Data (Dilution: WB 1:1000-2000, IHC 1:100-200)

Application Data (Dilution: WB 1:1000-2000, IHC 1:100-200)

Application Data (Dilution: WB 1:1000-2000, IHC 1:100-200)

Application Data (Dilution: WB 1:1000-2000, IHC 1:100-200)

Application Data (Dilution: WB 1:1000-2000, IHC 1:100-200)

WB (Western Blot) (Dilution: WB 1:1000-2000, IHC 1:100-200)

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ERK1/2, Monoclonal Antibody (Cat# AAA28912)

Full Name

ERK1/2 mouse Monoclonal Antibody (1H4)

Gene Names

MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

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FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of MCF-7 cells using anti-DDX1 antibody (AAA19378).Overlay histogram showing MCF-7 cells stained with AAA19378 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- DDX1 Antibody (AAA19378, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistchemistry) (Figure 9. IHC analysis of DDX1 using anti-DDX1 antibody (AAA19378).DDX1 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-DDX1 Antibody (AAA19378) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 8. IHC analysis of DDX1 using anti-DDX1 antibody (AAA19378).DDX1 was detected in paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-DDX1 Antibody (AAA19378) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of DDX1 using anti-DDX1 antibody (AAA19378).DDX1 was detected in paraffin-embedded section of human renal carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-DDX1 Antibody (AAA19378) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of DDX1 using anti-DDX1 antibody (AAA19378).DDX1 was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-DDX1 Antibody (AAA19378) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of DDX1 using anti-DDX1 antibody (AAA19378).DDX1 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-DDX1 Antibody (AAA19378) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of DDX1 using anti-DDX1 antibody (AAA19378).DDX1 was detected in paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-DDX1 Antibody (AAA19378) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of DDX1 using anti-DDX1 antibody (AAA19378).DDX1 was detected in paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-DDX1 Antibody (AAA19378) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of DDX1 using anti-DDX1 antibody (AAA19378).DDX1 was detected in paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-DDX1 Antibody (AAA19378) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of DDX1 using anti-DDX1 antibody (AAA19378).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human K562 whole cell lysatesLane 2: human CACO-2 whole cell lysatesLane 3: human U20S whole cell lysatesLane 4: human HEK293 whole cell lysatesLane 5: human U87 whole cell lysatesLane 6: human HELA whole cell lysatesLane 7: human A549 whole cell lysatesLane 8: rat heart tissue lysatesLane 9: rat kidney tissue lysatesLane 10: rat skeletal muscle tissue lysatesLane 11: mouse kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- DDX1 antigen affinity purified monoclonal antibody (Catalog # AAA19378) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for DDX1 at approximately 86KD. The expected band size for DDX1 is at 86KD.)

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DDX1, Monoclonal Antibody (Cat# AAA19378)

Full Name

Anti-DDX1 Antibody (monoclonal, 3I10)

Gene Names

DDX1; DBP-RB; UKVH5d

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, FC/FACS/FCM

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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WB (Western Blot) (Detection of human LAG3 by western blot. Samples: Whole cell lysate (50 ug) from HDLM-2, RPMI-8226, Jurkat, MJ, and HEK293T cells prepared using NETN lysis buffer. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody (AAA23801 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody .)

IP (Immunoprecipitation) (Detection of human LAG3 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HDLM-2 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-LAG3 recombinant monoclonal antibody (AAA23801 lot 2) used for IP at 12 ul per reaction. LAG3 was also immunoprecipitated by a previous lot of this antibody (lot AAA23801-1). For blotting immunoprecipitated LAG3, AAA23801 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)

IHC (Immunohistochemistry) (Detection of human LAG3 (red) by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody (AAA23801 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI (blue).)

IHC (Immunohistochemistry) (Detection of human LAG3 in FFPE tonsil by immunohistochemistry. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody (AAA23801 lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry) (Detection of human LAG3 in FFPE HDLM-2 by immunocytochemistry. Antibody: Rabbit anti-LAG3 recombinant monoclonal antibody (AAA23801 lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

FCM (Flow Cytometry) (Detection of human Lag3 (shaded) in HDLM-2 cells by flow cytometry. Antibody: Rabbit anti-Lag3 recombinant monoclonal antibody (AAA23801) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)

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LAG3, Monoclonal Recombinant Antibody (Cat# AAA23801)

Full Name

Rabbit anti-LAG3 Recombinant Monoclonal Antibody [BLR027F]

Gene Names

LAG3; CD223

Reactivity

Human

Applications

WB, IP, IHC, ICC, IHC-IF, mIF

Purity

Purified

Pricing

$295/0.01 mL $680/0.1 mL $980/5x0.01 mL $1,300/2x0.1 mL $1,890/3x0.1 mL $2,440/4x0.1 mL $2,755/5x0.1 mL

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FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of A431 cells using anti-SP1 antibody (AAA19656).Overlay histogram showing A431 cells stained with AAA19656 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-SP1 Antibody (AAA19656, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 9. IF analysis of SP1 using anti-SP1 antibody (AAA19656).SP1 was detected in an immunocytochemical section of HeLa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL mouse anti-SP1 Antibody (AAA19656) overnight at 4 degree C. DyLight550 Conjugated Goat Anti-Mouse IgG (BA1133) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 8. IHC analysis of SP1 using anti-SP1 antibody (AAA19656).SP1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-SP1 Antibody (AAA19656) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of SP1 using anti-SP1 antibody (AAA19656).SP1 was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-SP1 Antibody (AAA19656) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of SP1 using anti-SP1 antibody (AAA19656).SP1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-SP1 Antibody (AAA19656) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of SP1 using anti-SP1 antibody (AAA19656).SP1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-SP1 Antibody (AAA19656) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of SP1 using anti-SP1 antibody (AAA19656).SP1 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-SP1 Antibody (AAA19656) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of SP1 using anti-SP1 antibody (AAA19656).SP1 was detected in a paraffin-embedded section of human glioma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-SP1 Antibody (AAA19656) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of SP1 using anti-SP1 antibody (AAA19656).SP1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-SP1 Antibody (AAA19656) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of SP1 using anti-SP1 antibody (AAA19656).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human Caco-2 whole cell lysates,Lane 3: human Jurkat whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-SP1 antigen affinity purified monoclonal antibody (#AAA19656) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SP1 at approximately 90 kDa. The expected band size for SP1 is at 81 kDa.)

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SP1, Monoclonal Antibody (Cat# AAA19656)

Full Name

Anti-SP1 Antibody Picoband (monoclonal, 3C4C3)

Reactivity

Human

Applications

WB, IHC, ICC, IF, FC/FACS

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of U937 cells using anti-Rab11A antibody (AAA19685).Overlay histogram showing U937 cells stained with AAA19685 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Rab11A Antibody (AAA19685, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of RH35 cells using anti-Rab11A antibody (AAA19685).Overlay histogram showing RH35 cells stained with AAA19685 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Rab11A Antibody (AAA19685, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of ANA-1 cells using anti-Rab11A antibody (AAA19685).Overlay histogram showing ANA-1 cells stained with AAA19685 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Rab11A Antibody (AAA19685, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of Rab11A using anti-Rab11A antibody (AAA19685).Rab11A was detected in an immunocytochemical section of T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL mouse anti-Rab11A Antibody (AAA19685) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of Rab11A using anti-Rab11A antibody (AAA19685).Rab11A was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Rab11A Antibody (AAA19685) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of Rab11A using anti-Rab11A antibody (AAA19685).Rab11A was detected in a paraffin-embedded section of human gastric carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Rab11A Antibody (AAA19685) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of Rab11A using anti-Rab11A antibody (AAA19685).Rab11A was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Rab11A Antibody (AAA19685) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of Rab11A using anti-Rab11A antibody (AAA19685).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human placenta tissue lysates,Lane 3: human Colo320 lysates,Lane 4: human MDA-MB453 whole cell lysates,Lane 5: rat testis tissue lysates,Lane 6: rat brain tissue lysates,Lane 7: mouse testis tissue lysates,Lane 8: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Rab11A antigen affinity purified monoclonal antibody (#AAA19685) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Rab11A at approximately 22 kDa. The expected band size for Rab11A is at 22 kDa.)

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Rab11A, Monoclonal Antibody (Cat# AAA19685)

Full Name

Anti-Rab11A Antibody Picoband (monoclonal, 4H9)

Gene Names

RAB11A; YL8

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ICC, IF, FC/FACS

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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WB (Western Blot) (Western blot analysis of ARID3A over-expressed 293 cell line, cotransfected with ARID3A Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ARID3A monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged ARID3A is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ARID3A on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ARID3A on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml].)

WB (Western Blot) (ARID3A monoclonal antibody, Western Blot analysis of ARID3A expression in K-562.)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

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ARID3A, Monoclonal Antibody (Cat# AAA24729)

Full Name

ARID3A (AT-rich Interactive Domain-containing Protein 3A, ARID Domain-containing Protein 3A, B Cell Regulator of IgH Transcription, Bright, Dead Ringer-like Protein 1, E2F-binding Protein 1, DRIL1, DRIL3, DRX, E2FBP1) (Biotin)

Gene Names

ARID3A; DRIL1; DRIL3; BRIGHT; E2FBP1

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged PGR is ~0.3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PGR on MCF-7 cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody, 131852, to PGR on formalin-fixed paraffin-embedded human breast cancer. [antibody concentration 3ug/ml])

WB (Western Blot) (PGR monoclonal antibody, 131852, Western Blot analysis of PGR expression in MCF-7.)

WB (Western Blot) (PGR monoclonal antibody, 131852, Western Blot analysis of PGR expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen, 131852 (37.84kD))

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Progesterone Receptor, Monoclonal Antibody (Cat# AAA25509)

Full Name

Progesterone Receptor (PgR, PR, Nuclear Receptor Subfamily 3 Group C Member 3, NR3C3) (HRP)

Gene Names

PGR; PR; NR3C3

Reactivity

Human, Rat

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged FGR is ~0.3ng/ml as a capture antibody.)

IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to FGR on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3ug/ml.)

WB (Western Blot) (Western Blot analysis of FGR expression in transfected 293T cell line by FGR monoclonal antibody. Lane 1: FGR transfected lysate (59.5kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (FGR monoclonal antibody. Western Blot analysis of FGR expression in Raw 264.7.)

WB (Western Blot) (FGR monoclonal antibody. Western Blot analysis of FGR expression in PC-12.)

WB (Western Blot) (FGR monoclonal antibody. Western Blot analysis of FGR expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)

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FGR, Monoclonal Antibody (Cat# AAA24506)

Full Name

FGR (Tyrosine-protein Kinase Fgr, Gardner-Rasheed Feline Sarcoma Viral (v-fgr) Oncogene Homolog, Proto-oncogene c-Fgr, p55-Fgr, p58-Fgr, p58c-Fgr, SRC2) APC

Gene Names

FGR; SRC2; c-fgr; c-src2; p55-Fgr; p58-Fgr; p55c-fgr; p58c-fgr

Reactivity

Human, Mouse, Rat

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged UBE2C is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to UBE2C on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to UBE2C on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of UBE2C expression in transfected 293T cell line by UBE2C monoclonal antibody. Lane 1: UBE2C transfected lysate (19.7kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (UBE2C monoclonal antibody, Western Blot analysis of UBE2C expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (38.21kD).)

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UBE2C, Monoclonal Antibody (Cat# AAA25287)

Full Name

UBE2C (Ubiquitin-conjugating Enzyme E2 C, UbcH10, Ubiquitin Carrier Protein C, Ubiquitin-protein Ligase C, dJ447F3.2) (FITC)

Gene Names

UBE2C; UBCH10; dJ447F3.2

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (DARS monoclonal antibody Western Blot analysis of DARS expression in HeLa.)

WB (Western Blot) (Western blot analysis of DARS over-expressed 293 cell line, cotransfected with DARS Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with DARS monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged DARS is ~0.3ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to DARS on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of DARS expression in transfected 293T cell line by DARS monoclonal antibody. Lane 1: DARS transfected lysate (57.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (37.62kD).)

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DARS, Monoclonal Antibody (Cat# AAA24776)

Full Name

DARS (Aspartyl-tRNA Synthetase, Cytoplasmic, Aspartate-tRNA Ligase, AspRS, Cell Proliferation-inducing Gene 40 Protein, PIG40) (Biotin)

Gene Names

DARS; HBSL; aspRS

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (MAN1B1 monoclonal antibody Western Blot analysis of MAN1B1 expression in LNCaP.)

Application Data (Detection limit for recombinant GST tagged MAN1B1 is ~0.03ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to MAN1B1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3ug/ml])

WB (Western Blot) (MAN1B1 monoclonal antibody Western Blot analysis of MAN1B1 expression in A-431.)

WB (Western Blot) (MAN1B1 monoclonal antibody Western Blot analysis of MAN1B1 expression in HepG2.)

WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)

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MAN1B1, Monoclonal Antibody (Cat# AAA24559)

Full Name

MAN1B1 (Endoplasmic Reticulum Mannosyl-oligosaccharide 1,2-alpha-mannosidase, ER alpha-1,2-Mannosidase, ER Mannosidase 1, ERMan1, Man9GlcNAc2-specific-processing alpha-mannosidase, Mannosidase alpha Class 1B Member 1, UNQ747/PRO1477) APC

Gene Names

MAN1B1; MRT15; ERMAN1; MANA-ER

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Staining of human peripheral blood lymphocytes with RAT ANTI HUMAN CD28:RPE)

Application Data (Staining of human peripheral blood lymphocytes with RAT ANTI HUMAN CD28:FITC)

Application Data (Staining of human peripheral blood lymphocytes with RAT ANTI HUMAN CD28: AZIDE FREE)

Application Data (Staining of human peripheral blood lymphocytes with RAT ANTI HUMAN CD28: Alexa Fluor® 647)

Application Data (Staining of human peripheral blood lymphocytes with RAT ANTI HUMAN CD28)

Application Data (MCA709B staining of human peripheral blood lymphocytes)

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CD28, Monoclonal Antibody (Cat# AAA26807)

Full Name

CD28 (CD28 Antigen, CD28 Molecule, MGC138290, T cell Antigen CD28, T cell Specific Surface Glycoprotein, T cell Specific Surface Glycoprotein CD28, Tp44) (MaxLight 550)

Reactivity

Human

Applications

FC/FACS, IHC

Purity

Purified by Protein G Affinity Chromatography

Pricing

$1,015/0.1 mL $1,960/2x0.1 mL $2,850/3x0.1 mL $3,690/4x0.1 mL $4,295/5x0.1 mL

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WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)

WB (Western Blot) (Western blot analysis of ASNA1 over-expressed 293 cell line, cotransfected with ASNA1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ASNA1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged ASNA1 is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ASNA1 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ASNA1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 0.5ug/ml])

WB (Western Blot) (Western Blot analysis of ASNA1 expression in transfected 293T cell line by ASNA1 monoclonal antibody. Lane 1: ASNA1 transfected lysate (38.8kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (ASNA1 monoclonal antibody. Western Blot analysis of ASNA1 expression in PC-12.)

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ASNA1, Monoclonal Antibody (Cat# AAA25030)

Full Name

ASNA1 (ATPase ASNA1, Arsenical Pump-driving ATPase, Arsenite-stimulated ATPase, Transmembrane Domain Recognition Complex 40kD ATPase Subunit, hARSA-I, hASNA-I, ARSA, TRC40) (FITC)

Gene Names

ASNA1; GET3; ARSA1; TRC40; ARSA-I; ASNA-I

Reactivity

Human, Rat

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western Blot detection against Immunogen (51.96kD).)

Application Data (Detection limit for recombinant GST tagged FAM3B is 1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of FAM3B transfected lysate using FAM3B monoclonal antibody and Protein A Magnetic Bead and immunoblotted with FAM3B rabbit polyclonal antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FAM3B on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of FAM3B expression in transfected 293T cell line by FAM3B monoclonal antibody.Lane 1: FAM3B transfected lysate (26kD).Lane 2: Non-transfected lysate.)

WB (Western Blot) (FAM3B monoclonal antibody. Western Blot analysis of FAM3B expression in human kidney.)

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FAM3B, Monoclonal Antibody (Cat# AAA14793)

Full Name

FAM3B (Protein FAM3B, Cytokine-like Protein 2-21, Pancreatic-derived Factor, PANDER, UNQ320/PRO365, PRED44, C21orf76, C21orf11, D21M16SJHU19e)

Reactivity

Human

Applications

EL/EIA, WB, IP, IHC

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

$820/0.1 mg $1,565/2x0.1 mg $2,270/3x0.1 mg $2,940/4x0.1 mg $3,380/5x0.1 mg

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IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to PDK2 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 2 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PDK2 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 2 ug/ml])

Application Data (Detection limit for recombinant GST tagged PDK2 is approximately 0.03ng/ml as a capture antibody.)

WB (Western Blot) (PDK2 monoclonal antibody (M02), clone 5F8. Western Blot analysis of PDK2 expression in A-431 (Cat # L015V1).)

WB (Western Blot) (PDK2 monoclonal antibody (M02), clone 5F8. Western Blot analysis of PDK2 expression in 293 (Cat # L026V1).)

WB (Western Blot) (PDK2 monoclonal antibody (M02), clone 5F8 Western Blot analysis of PDK2 expression in HeLa (Cat # L013V1).)

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PDK2, Monoclonal Antibody (Cat# AAA26595)

Full Name

PDK2 (Pyruvate Dehydrogenase Kinase, Isozyme 2, PDHK2) (PE)

Gene Names

PDK2; PDHK2; PDKII

Applications

IHC, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (SMAD1 monoclonal antibody Western Blot analysis of SMAD1 expression in A-549.)

WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)

Application Data (Detection limit for recombinant GST tagged SMAD1 is ~3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SMAD1 on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SMAD1 on formalin-fixed paraffin-embedded human salivary gland tissue. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of SMAD1 expression in transfected 293T cell line by SMAD1 monoclonal antibody. Lane 1: SMAD1 transfected lysate (52.3kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (SMAD1 monoclonal antibody. Western Blot analysis of SMAD1 expression in MES-SA/Dx5.)

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SMAD1, Monoclonal Antibody (Cat# AAA25839)

Full Name

SMAD1 (Mothers Against Decapentaplegic Homolog 1, Mothers Against DPP Homolog 1, MAD Homolog 1, MADH1, SMAD Family Member 1, SMAD 1, hSMAD1, BSP-1, BSP1, JV4-1, JV41, Mad-related Protein 1, MADR1, Transforming Growth Factor-beta-signaling Protein 1) (PE)

Gene Names

SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of CTNNB1 expression in transfected 293T cell line by CTNNB1 monoclonal antibody. Lane 1: CTNNB1 transfected lysate (85.5kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between FLT1 and CTNNB1. Huh7 cells were stained with FLT1 rabbit purified polyclonal 1:1200 and CTNNB1 mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between GSK3B and CTNNB1. HeLa cells were stained with GSK3B rabbit purified polyclonal 1:1200 and CTNNB1 mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

WB (Western Blot) (Western blot analysis of CTNNB1 over-expressed 293 cell line, cotransfected with CTNNB1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CTNNB1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged CTNNB1 is 0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CTNNB1 on HeLa cell. [antibody concentration 10ug/ml].)

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CTNNB1, Monoclonal Antibody (Cat# AAA25069)

Full Name

CTNNB1 (Catenin beta-1, Beta-catenin, CTNNB, OK/SW-cl.35, PRO2286) (FITC)

Gene Names

CTNNB1; EVR7; CTNNB; MRD19; NEDSDV; armadillo

Reactivity

Human

Applications

EIA, IF, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (VAX1 monoclonal antibody Western Blot analysis of VAX1 expression in NIH/3T3.)

WB (Western Blot) (Western Blot analysis of VAX1 expression in transfected 293T cell line by VAX1 monoclonal antibody Lane 1: VAX1 transfected lysate (21kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (VAX1 monoclonal antibody Western Blot analysis of VAX1 expression in Raw 264.7.)

WB (Western Blot) (VAX1 monoclonal antibody Western Blot analysis of VAX1 expression in HepG2.)

WB (Western Blot) (VAX1 monoclonal antibody Western Blot analysis of VAX1 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)

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VAX1, Monoclonal Antibody (Cat# AAA25586)

Full Name

VAX1 (Ventral Anterior Homeobox 1, MCOPS11) (HRP)

Reactivity

Human, Mouse, Rat

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

WB (Western Blot) (Western Blot; Sample: Gallus OVA;Primary Ab: 0.5ug/ml Mouse Anti-Multi-species OVA AntibodySecond Ab: 0.2 ug/ml HRP-Linked Caprine Anti-Mouse IgG Polyclonal Antibody(Catalog:))

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Ovalbumin, Monoclonal Antibody (Cat# AAA20826)

Full Name

Monoclonal Antibody to Ovalbumin (OVA)

Reactivity

General

Applications

WB, IHC, ICC, IP

Purity

Protein A + Protein G affinity chromatography

Pricing

$320/0.1 mL $395/0.2 mL $690/0.5 mL $840/1 mL $2,305/5 mL $4,470/2x5 mL $6,510/3x5 mL $8,445/4x5 mL $10,310/5x5 mL

What are Monoclonal Antibodies?

Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.

This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.

AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.

What Are The Uses of Monoclonal Antibodies

Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.

ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.

Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.

Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.

Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.

How Monoclonal Antibodies Are Used as Medicine?

Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).

Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:

• Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.


• Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.


• Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.

Why Buy Monoclonal Antibodies From Us?

At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.

1. Validated for Versatile Applications

The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).

2. Wide Selection & Specialized Options

We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.

3. High-Quality Proteins

Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.

Frequently Asked Questions

1. Are your monoclonal antibodies validated for specific applications?

Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.

2. How do I choose the right monoclonal antibody for my application?

Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.

3. How quickly can I receive my order?

Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.