Monoclonal Antibodies

Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.

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Application Data (Detection limit for recombinant GST tagged PLXNA2 is 0.1ng/ml as a capture antibody.)

WB (Western Blot) (Western Blot analysis of PLXNA2 expression in transfected 293T cell line by PLXNA2 monoclonal antibody. Lane 1: PLXNA2 transfected lysate (Predicted MW: 18.8kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (PLXNA2 monoclonal antibody. Western Blot analysis of PLXNA2 expression in NIH/3T3.)

WB (Western Blot) (PLXNA2 monoclonal antibody. Western Blot analysis of PLXNA2 expression in Raw 264.7.)

WB (Western Blot) (PLXNA2 monoclonal antibody. Western Blot analysis of PLXNA2 expression in HepG2)

WB (Western Blot) (PLXNA2 monoclonal antibody. Western Blot analysis of PLXNA2 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (43.78kD).)

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PLXNA2, Monoclonal Antibody (Cat# AAA25504)

Full Name

PLXNA2 (Plexin A2, KIAA0463, OCT, PLXN2, Plexin-A2, Semaphorin Receptor OCT, FLJ11751, FLJ30634) (HRP)

Gene Names

PLXNA2; OCT; PLXN2

Reactivity

Human, Mouse, Rat

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Proximity Ligation Analysis of protein-protein interactions between CRKL and PIK3R1. Mahlavu cells were stained with anti-CRKL rabbit purified polyclonal 1:1200 and anti-PIK3R1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)

Application Data (Proximity Ligation Analysis of protein-protein interactions between FLT1 and PIK3R1 Huh7 cells were stained with anti-FLT1 rabbit purified polyclonal 1:1200 and anti-PIK3R1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)

Application Data (Proximity Ligation Analysis of protein-protein interactions between EGFR and PIK3R1. HeLa cells were stained with EGFR rabbit purified polyclonal 1:1200 and PIK3R1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue))

Application Data (Detection limit for recombinant GST tagged PIK3R1 is ~0.1ng/ml as a capture antibody.)

WB (Western Blot) (PIK3R1 monoclonal antibody Western Blot analysis of PIK3R1 expression in PC-12.)

WB (Western Blot) (PIK3R1 monoclonal antibody Western Blot analysis of PIK3R1 expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (75.68kD).)

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PIK3R1, Monoclonal Antibody (Cat# AAA25794)

Full Name

PIK3R1 (GRB1, Phosphatidylinositol 3-kinase Regulatory Subunit alpha, Phosphatidylinositol 3-kinase 85kD Regulatory Subunit alpha) (PE)

Gene Names

PIK3R1; p85; AGM7; GRB1; IMD36; p85-ALPHA

Reactivity

Human, Mouse, Rat

Applications

EIA, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (CA1 monoclonal antibody, Western Blot analysis of CA1 expression in HeLa.)

Application Data (Detection limit for recombinant GST tagged CA1 is ~0.03ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of CA1 transfected lysate using 124177 and Protein A Magnetic Bead and immunoblotted with CA1 rabbit polyclonal antibody.)

WB (Western Blot) (Western Blot analysis of CA1 expression in transfected 293T cell line using 124177. Lane 1: CA1 transfected lysate (28.9kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot analysis of CA1 expression in human spleen using 124177)

WB (Western Blot) (Western Blot detection against Immunogen (54.45kD), using 124177.)

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CA1, Monoclonal Antibody (Cat# AAA24742)

Full Name

CA1 (Carbonic Anhydrase 1, Carbonic Anhydrase I, CA-I, Carbonate Dehydratase I, Carbonic Anhydrase B, CAB) (Biotin)

Gene Names

CA1; CAB; CA-I; Car1; HEL-S-11

Reactivity

Human

Applications

EIA, IP, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsils using CD23 (3D1) Mouse mAb.)

IHC (Immunohistchemistry) (IHC staining of Human tonsil tissue paraffin-embedded, diluted at 1:200.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Mouse-lung tissue. 1,CD23 Monoclonal Antibody(1E9) was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,CD23 Monoclonal Antibody(1E9) was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human-lung tissue. 1,CD23 Monoclonal Antibody(1E9) was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)

IF (Immunofluorescence) (Immunofluorescence analysis of rat-lung tissue. 1,CD23 Monoclonal Antibody(1E9)(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

IF (Immunofluorescence) (Immunofluorescence analysis of human-stomach tissue. 1,CD23 Monoclonal Antibody(1E9)(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

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CD23, Monoclonal Antibody (Cat# AAA23992)

Full Name

CD23 (3D1) Mouse mAb

Gene Names

FCER2; CD23; FCE2; CD23A; IGEBF; CLEC4J; BLAST-2

Reactivity

Human, Mouse, Rat

Applications

ICC, IF, IHC-P, IHC-F

Purity

Affinity purified

Pricing

$565/0.1 mL $1,070/2x0.1 mL $1,545/3x0.1 mL $1,900/5x0.1 mL $1,995/4x0.1 mL

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Application Data (Proximity Ligation Analysis of protein-protein interactions between STAT1 and PDGFRB Mahlavu cells were stained with anti-STAT1 rabbit purified polyclonal (1:1200) and 131071 (1:50). Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)

Application Data (Detection limit for recombinant GST tagged PDGFRB is ~1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of PDGFRB transfected lysate using 131071 and Protein A Magnetic Bead, and immunoblotted with PDGFRB rabbit polyclonal antibody.)

WB (Western Blot) (Western Blot analysis of PDGFRB expression in human uterus myoma using 131071.)

WB (Western Blot) (Western Blot analysis of PDGFRB expression in human stomach using 131071.)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

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PDGFRB, Monoclonal Antibody (Cat# AAA25784)

Full Name

PDGFRB (PDGFR, PDGFR1, Platelet-derived Growth Factor Receptor beta, Beta Platelet-derived Growth Factor Receptor, Beta-type Platelet-derived Growth Factor Receptor, CD140 Antigen-like Family Member B, Platelet-derived Growth Factor Receptor 1, CD140b) (P

Gene Names

PDGFRB; IMF1; KOGS; IBGC4; JTK12; PDGFR; PENTT; CD140B; PDGFR1; PDGFR-1

Reactivity

Human

Applications

EIA, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CDC2 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CDC2 on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (CDC2 monoclonal antibody (M03), clone 1G10. Western Blot analysis of CDC2 expression in Raw 264.7.)

WB (Western Blot) (CDC2 monoclonal antibody (M03), clone 1G10. Western Blot analysis of CDC2 expression in PC-12.)

WB (Western Blot) (CDC2 monoclonal antibody (M03), clone 1G10. Western Blot analysis of CDC2 expression in NIH/3T3.)

WB (Western Blot) (CDC2 monoclonal antibody (M03), clone 1G10 Western Blot analysis of CDC2 expression in Hela S3 NE.)

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CDC2, Monoclonal Antibody (Cat# AAA26057)

Full Name

CDC2 (Cell Division Cycle 2, G1 to S and G2 to M, CDC28A, CDK1, DKFZp686L20222, MGC111195) (APC)

Gene Names

CDK1; CDC2; CDC28A; P34CDC2

Applications

EIA, IF, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug NF-kB p65/RelA antibody . Western blot was performed from the immunoprecipitate using NF-kB p65/RelA antibody at a dilition of 1:1000.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using NF-kB p65/RelA antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat spleen using NF-kB p65/RelA antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using NF-kB p65/RelA antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 3min.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using NF-kB p65/RelA antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 3min.)

WB (Western Blot) (Western blot analysis of extracts from normal (control) and NF-kB p65/RelA knockout (KO) HeLa cells, using NF-kB p65/RelA antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 3min.)

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NF-kB p65/RelA, Monoclonal Antibody (Cat# AAA28381)

Full Name

[KO Validated] NF-kB p65/RelA Rabbit mAb

Gene Names

RELA; p65; NFKB3

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IP

Purity

Affinity purification

Pricing

$300/0.02 mL $500/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,780/4x0.1 mL $1,960/5x0.1 mL

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WB (Western Blot) (Western blot analysis of ORM1 over-expressed 293 cell line, cotransfected with ORM1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ORM1 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged ORM1 is ~0.1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of ORM1 transfected lysate using ORM1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ORM1 rabbit polyclonal antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ORM1 on formalin-fixed paraffin-embedded human stomach carcinoma tissue. [antibody concentration 1ug/ml])

WB (Western Blot) (Western Blot analysis of ORM1 expression in transfected 293T cell line by ORM1 monoclonal antibody. Lane 1: ORM1 transfected lysate (23.5kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (ORM1 monoclonal antibody Western Blot analysis of ORM1 expression in MCF-7.)

WB (Western Blot) (Western Blot detection against Immunogen (45.98kD).)

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AGP1, Monoclonal Antibody (Cat# AAA25310)

Full Name

AGP1 (Alpha-1-acid Glycoprotein 1, AGP 1, Orosomucoid-1, OMD 1, ORM1) (HRP)

Gene Names

ORM1; ORM; AGP1; AGP-A; HEL-S-153w

Reactivity

Human

Applications

EIA, IHC, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of LYPLA3 expression in NIH/3T3.)

WB (Western Blot) (Western Blot analysis of PLA2G15 expression in transfected 293T cell line)

WB (Western Blot) (Western Blot analysis of LYPLA3 expression in Raw 264.7)

WB (Western Blot) (Western Blot analysis of LYPLA3 expression in PC-12)

WB (Western Blot) (Western Blot analysis of LYPLA3 expression in HeLa)

WB (Western Blot) (Western Blot detection against Immunogen using (37kD).)

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LYPLA3, Monoclonal Antibody (Cat# AAA24556)

Full Name

LYPLA3 (PLA2G15, Group XV Phospholipase A2, 1-O-acylceramide Synthase, ACS, LCAT-like Lysophospholipase, LLPL, Lysophospholipase 3, Lysosomal Phospholipase A2, LPLA2, UNQ341/PRO540) APC

Gene Names

PLA2G15; ACS; LLPL; LPLA2; LYPLA3; GXVPLA2

Reactivity

Human, Mouse, Rat

Applications

WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged HMGB2 is approximately 0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HMGB2 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HMGB2 on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (HMGB2 monoclonal antibody (M05), clone 3E5 Western Blot analysis of HMGB2 expression in Hela S3 NE.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HMGB2 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to HMGB2 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml])

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HMGB2, Monoclonal Antibody (Cat# AAA26093)

Full Name

HMGB2 (High-Mobility Group Box 2, HMG2) (APC)

Applications

IF, IHC, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HDAC1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of HDAC1 expression in transfected 293T cell line by HDAC1 monoclonal antibody Lane 1: HDAC1 transfected lysate (55.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (HDAC1 monoclonal antibody Western Blot analysis of HDAC1 expression in NIH/3T3)

WB (Western Blot) (HDAC1 monoclonal antibody Western Blot analysis of HDAC1 expression in Raw 264.7)

WB (Western Blot) (HDAC1 monoclonal antibody Western Blot analysis of HDAC1 expression in Hela NE)

WB (Western Blot) (HDAC1 monoclonal antibody. Western Blot analysis of HDAC1 expression in PC-12)

WB (Western Blot) (Western Blot detection against Immunogen (78.76kD).)

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HDAC1, Monoclonal Antibody (Cat# AAA24826)

Full Name

HDAC1 (Histone Deacetylase 1, HD1, HD-1, HDAC1, HDAC-1, RPD3, RPD3L1, Reduced Potassium Dependency 3 Like-1) (Biotin)

Gene Names

HDAC1; HD1; RPD3; GON-10; RPD3L1

Reactivity

Human, Mouse, Rat

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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SDS-PAGE (SDS-PAGE Analysis Purified CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/2598R). Confirmation of Purity and Integrity of Antibody.)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Tonsil stained with CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/2598R).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/2598R).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/2598R).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/2598R).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Angiosarcoma stained with CD34 Recombinant Rabbit Monoclonal Antibody (HPCA1/2598R).)

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CD34, Monoclonal Antibody (Cat# AAA23910)

Full Name

CD34 (Hematopoietic Stem Cell & Endothelial Marker)

Reactivity

Human, Rat. Others not known.

Applications

Immunohistochemistry

Purity

Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Pricing

$295/0.02 mg (With BSA & Azide at 0.2mg/mL) $475/0.1 mg (With BSA & Azide at 0.2mg/mL) $475/0.1 mg (Without BSA & Azide at 1mg/mL) $905/2x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,310/3x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,690/4x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,900/5x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,900/5x0.1 mg (Without BSA & Azide at 1mg/mL)

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WB (Western Blot) (CD58 monoclonal antibody, Western Blot analysis of CD58 expression in Jurkat.)

Application Data (Detection limit for recombinant GST tagged CD58 is ~0.3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CD58 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CD58 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 2ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CD58 on formalin-fixed paraffin-embedded human lymphoma tissue [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot detection against Immunogen (52.14kD).)

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CD58, Monoclonal Antibody (Cat# AAA24751)

Full Name

CD58 (Lymphocyte Function-associated Antigen 3, Ag3, Surface Glycoprotein LFA-3, LFA3) (Biotin)

Gene Names

CD58; ag3; LFA3; LFA-3

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged SORD is ~0.1ng/ml as a capture antibody.)

IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to SORD on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1.2ug/ml].)

WB (Western Blot) (Western Blot analysis of SORD expression in transfected 293T cell line by SORD monoclonal antibody. Lane 1: SORD transfected lysate (38.165kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (SORD monoclonal antibody. Western Blot analysis of SORD expression in Raw 264.7.)

WB (Western Blot) (SORD monoclonal antibody, Western Blot analysis of SORD expression in Hela NE.)

WB (Western Blot) (SORD monoclonal antibody. Western Blot analysis of SORD expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (38.21kD).)

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SORD, Monoclonal Antibody (Cat# AAA25259)

Full Name

SORD (Sorbitol Dehydrogenase, L-iditol 2-dehydrogenase) (FITC)

Gene Names

SORD; SORD1; HEL-S-95n

Reactivity

Human, Mouse, Rat

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of GLUL expression in transfected 293T cell line by GLUL monoclonal antibody (M02), clone 3B6.Lane 1: GLUL transfected lysate(42.1 KDa).Lane 2: Non-transfected lysate.)

WB (Western Blot) (GLUL monoclonal antibody (M02), clone 3B6. Western Blot analysis of GLUL expression in Raw 264.7.)

WB (Western Blot) (GLUL monoclonal antibody (M02), clone 3B6. Western Blot analysis of GLUL expression in PC-12.)

Application Data (Detection limit for recombinant GST tagged GLUL is approximately 0.03ng/ml as a capture antibody.)

WB (Western Blot) (GLUL monoclonal antibody (M02), clone 3B6. Western Blot analysis of GLUL expression in HepG2.)

WB (Western Blot) (GLUL monoclonal antibody (M02), clone 3B6 Western Blot analysis of GLUL expression in Jurkat.)

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GLUL, Monoclonal Antibody (Cat# AAA26027)

Full Name

GLUL (Glutamate-Ammonia Ligase (Glutamine Synthetase), GLNS, GS, PIG43, PIG59) (APC)

Gene Names

GLUL; GS; GLNS; PIG43; PIG59

Applications

WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western blot analysis of Human Lysates showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:1000. Comparison of clone H9010 behavior with Hsp90 human beta (1) and Hsp90 human alpha (2). Courtesy of: David Toft, Mayo Clinic.)

WB (Western Blot) (Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:1000. Secondary Antibody: HRP Goat Anti-Mouse.)

IHC (Immunohistchemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Alexa Fluor 555 Goat Anti-Mouse (red) at 1:5000 for 1 hour at RT. Magnification: 40x.)

WB (Western Blot) (Western Blot analysis of Human cell lysates from various cell lines showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Alexa Fluor 555 Goat Anti-Mouse (red) at 1:5000 for 1 hour at RT. Localization: Inflammatory and epithelial mucosa. Magnification: 40x. Inflammatory and epithelial mucosa.)

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Hsp90, Monoclonal Antibody (Cat# AAA17792)

Full Name

Hsp90 Antibody: ATTO 594

Gene Names

HSP90AB1; HSP84; HSPC2; HSPCB; D6S182; HSP90B

Reactivity

Human (beta-specific), Rabbit (Beta Specific), Chicken (Alpha/Beta), Rat, Canine

Applications

WB, IP, EIA, IHC, IF

Pricing

$680/0.2 mg $1,300/2x0.2 mg $1,890/3x0.2 mg $2,440/4x0.2 mg $2,685/5x0.2 mg

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WB (Western Blot) (BACE1 monoclonal antibody (M02), clone 2C1. Western Blot analysis of BACE1 expression in rat testis.)

WB (Western Blot) (Western Blot analysis of BACE1 expression in transfected 293T cell line by BACE1 monoclonal antibody (M01), clone 2C1.Lane 1: BACE1 transfected lysate(52.9 KDa).Lane 2: Non-transfected lysate.)

Application Data (Proximity Ligation Analysis of protein-protein interactions between MMP2 and BACE1. HeLa cells were stained with anti-MMP2 rabbit purified polyclonal 1:1200 and anti-BACE1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)

Application Data (Detection limit for recombinant GST tagged BACE1 is approximately 0.03ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to BACE1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to BACE1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml])

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BACE1, Monoclonal Antibody (Cat# AAA26019)

Full Name

BACE1 (beta-site APP-cleaving Enzyme 1, ASP2, BACE, FLJ90568, HSPC104, KIAA1149) (AP)

Gene Names

BACE1; ASP2; BACE; HSPC104

Applications

IHC, WB, Cell

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (C22orf18 monoclonal antibody, Western Blot analysis of C22orf18 expression in K-562.)

Application Data (Detection limit for recombinant GST tagged C22orf18 is ~0.03ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of CENPM transfected lysate using CENPM monoclonal antibody and Protein A Magnetic Bead and immunoblotted with CENPM rabbit polyclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MGC861 on HeLa cell. [antibody concentration 10ug/ml].)

WB (Western Blot) (Western Blot analysis of CENPM expression in transfected 293T cell line by C22orf18 monoclonal antibody. Lane 1: CENPM transfected lysate (19.7kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (45.91kD).)

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CENPM, Monoclonal Antibody (Cat# AAA24741)

Full Name

CENPM (C22orf18, ICEN39, PANE1, Centromere Protein M, Interphase Centromere Complex Protein 39, Proliferation-associated Nuclear Element Protein 1, MGC861, PANE1, BK250D10.2) (Biotin)

Gene Names

CENPM; PANE1; CENP-M; C22orf18

Reactivity

Human

Applications

EIA, IF, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western Blot detection against Immunogen (37.44kD).)

WB (Western Blot) (CCT7 monoclonal antibody Western Blot analysis of CCT7 expression in human pancreas.)

Application Data (Detection limit for recombinant GST tagged CCT7 is ~0.3ng/ml as a capture antibody.)

WB (Western Blot) (Western Blot analysis of CCT7 expression in transfected 293T cell line by CCT7 monoclonal antibody. Lane 1: CCT7 transfected lysate (59.4kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (CCT7 monoclonal antibody Western Blot analysis of CCT7 expression in K-562.)

WB (Western Blot) (CCT7 monoclonal antibody Western Blot analysis of CCT7 expression in Raw 264.7)

WB (Western Blot) (CCT7 monoclonal antibody Western Blot analysis of CCT7 expression in HL-60)

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CCT7, Monoclonal Antibody (Cat# AAA25046)

Full Name

CCT7 (T-complex Protein 1 Subunit eta, TCP-1-eta, CCT-eta, HIV-1 Nef-interacting Protein, CCTH, NIP7-1) (FITC)

Gene Names

CCT7; CCTH; CCTETA; NIP7-1; TCP1ETA

Reactivity

Human, Mouse, Rat

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (ASB10 monoclonal antibody. Western Blot analysis of ASB10 expression in NIH/3T3.)

Application Data (Detection limit for recombinant GST tagged ASB10 is ~0.1ng/ml as a capture antibody.)

WB (Western Blot) (ASB10 monoclonal antibody, Western Blot analysis of ASB10 expression in A-431.)

WB (Western Blot) (ASB10 monoclonal antibody. Western Blot analysis of ASB10 expression in Raw 264.7.)

WB (Western Blot) (ASB10 monoclonal antibody. Western Blot analysis of ASB10 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (37.77kD).)

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ASB10, Monoclonal Antibody (Cat# AAA24043)

Full Name

ASB10 (Ankyrin Repeat and SOCS Box Protein 10, ASB-10)

Gene Names

ASB10; GLC1F

Reactivity

Human, Mouse, Rat

Applications

EL/EIA, WB

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

$820/0.1 mg $1,565/2x0.1 mg $2,270/3x0.1 mg $2,940/4x0.1 mg $3,380/5x0.1 mg

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WB (Western Blot) (ATP2C1 monoclonal antibody Western Blot analysis of ATP2C1 expression in HeLa.)

WB (Western Blot) (Western blot analysis of ATP2C1 over-expressed 293 cell line, cotransfected with ATP2C1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ATP2C1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged ATP2C1 is ~0.03ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of ATP2C1 transfected lysate using ATP2C1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with ATP2C1 rabbit polyclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ATP2C1 on HeLa cell. [antibody concentration 10ug/ml])

WB (Western Blot) (Western Blot analysis of ATP2C1 expression in transfected 293T cell line by ATP2C1 monoclonal antibody. Lane 1: ATP2C1 transfected lysate (100.6kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (42.61kD).)

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ATP2C1, Monoclonal Antibody (Cat# AAA24734)

Full Name

ATP2C1 (KIAA1347, PMR1L, Calcium-transporting ATPase Type 2C Member 1, ATP-dependent Ca(2+) Pump PMR1) (Biotin)

Gene Names

ATP2C1; HHD; BCPM; PMR1; SPCA1; hSPCA1; ATP2C1A

Reactivity

Human

Applications

EIA, IF, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of NQO1 expression in transfected 293T cell line by NQO1 monoclonal antibody. Lane 1: NQO1 transfected lysate (30.9kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot analysis of NQO1 expression in HepG2.)

WB (Western Blot) (Western blot analysis of NQO1 over-expressed 293 cell line, cotransfected with NQO1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NQO1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged NQO1 is ~0.3ng/ml as a capture antibody)

IP (Immunoprecipitation) (Immunoprecipitation of NQO1 transfected lysate using NQO1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with NQO1 rabbit polyclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NQO1 on HepG2 cell. [antibody concentration 10ug/ml])

WB (Western Blot) (Western Blot detection against Immunogen (55.88kD).)

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NQO1, Monoclonal Antibody (Cat# AAA25771)

Full Name

NQO1 (NAD(P)H Dehydrogenase [Quinone] 1, Azoreductase, DT-diaphorase, DTD, QR1, Menadione Reductase, NAD(P)H:quinone Oxidoreductase 1, Phylloquinone Reductase, Quinone Reductase 1, DIA4, NMOR1) (PE)

Gene Names

NQO1; DTD; QR1; DHQU; DIA4; NMOR1; NMORI

Reactivity

Human

Applications

EIA, IF, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (CDK2 monoclonal antibody. Western Blot analysis of CDK2 expression in Jurkat.)

WB (Western Blot) (Western blot analysis of CDK2 over-expressed 293 cell line, cotransfected with CDK2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CDK2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged CDK2 is ~0.03ng/ml as a capture antibody.)

WB (Western Blot) (Western Blot analysis of CDK2 expression in transfected 293T cell line by CDK2 monoclonal antibody. Lane 1: CDK2 transfected lysate (33.9kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (CDK2 monoclonal antibody, Western Blot analysis of CDK2 expression in HL-60.)

WB (Western Blot) (Western Blot detection against Immunogen (35.42kD).)

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CDK2, Monoclonal Antibody (Cat# AAA24755)

Full Name

CDK2 (Cell Division Protein Kinase 2, p33 Protein Kinase) (Biotin)

Gene Names

CDK2; CDKN2; p33(CDK2)

Reactivity

Human

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western Blot detection against Immunogen (78.03kD).)

WB (Western Blot) (POLK monoclonal antibody, Western Blot analysis of POLK expression in Hela NE.)

Application Data (Detection limit for recombinant GST tagged POLK is ~0.3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to POLK on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to POLK on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of POLK expression in transfected 293T cell line by POLK monoclonal antibody. Lane 1: POLK transfected lysate (54.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western blot analysis of POLK over-expressed 293 cell line, cotransfected with POLK Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with POLK monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

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POLK, Monoclonal Antibody (Cat# AAA25802)

Full Name

POLK (DNA Polymerase kappa, DINB1, DINB Protein) (PE)

Gene Names

POLK; DINP; POLQ; DINB1

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (BPNT1 monoclonal antibody Western Blot analysis of BPNT1 expression in HeLa.)

Application Data (Detection limit for recombinant GST tagged BPNT1 is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to BPNT1 on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to BPNT1 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of BPNT1 expression in transfected 293T cell line by BPNT1 monoclonal antibody. Lane 1: BPNT1 transfected lysate (28.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

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BPNT1, Monoclonal Antibody (Cat# AAA25035)

Full Name

BPNT1 (3'(2'),5'-bisphosphate nucleotidase 1, Bisphosphate 3'-nucleotidase 1, PAP-inositol 1,4-phosphatase) (FITC)

Gene Names

BPNT1; PIP; HEL20

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (SPRY2 monoclonal antibody Western Blot analysis of SPRY2 expression in C32)

Application Data (Detection limit for recombinant GST tagged SPRY2 is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SPRY2 on HeLa cell. [antibody concentration 25ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SPRY2 on formalin-fixed paraffin-embedded human lymphoma. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of SPRY2 expression in transfected 293T cell line by SPRY2 monoclonal antibody Lane 1: SPRY2 transfected lysate (34.7kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (60.76kD).)

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SPRY2, Monoclonal Antibody (Cat# AAA25556)

Full Name

SPRY2 (Protein Sprouty Homolog 2, Spry-2, hSPRY2) (HRP)

Gene Names

SPRY2; IGAN3; hSPRY2

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (TGIF2 monoclonal antibody, Western Blot analysis of TGIF2 expression in HeLa NE.)

WB (Western Blot) (TGIF2 monoclonal antibody. Western Blot analysis of TGIF2 expression in SW-13.)

WB (Western Blot) (Western blot analysis of TGIF2 over-expressed 293 cell line, cotransfected with TGIF2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TGIF2 monoclonal antibody (M01). GAPDH (36.1kD). used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged TGIF2 is ~0.3ng/ml as a capture antibody.)

WB (Western Blot) (Western Blot analysis of TGIF2 expression in transfected 293T cell line by TGIF2 monoclonal antibody. Lane 1: TGIF2 transfected lysate (25.9kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (TGIF2 monoclonal antibody. Western Blot analysis of TGIF2 expression in IMR-32.)

WB (Western Blot) (Western Blot detection against Immunogen (37.77kD).)

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TGIF2, Monoclonal Antibody (Cat# AAA25567)

Full Name

TGIF2 (TGF-beta-induced Transcription Factor 2, TGFB-induced Factor 2, Homeobox Protein TGIF2, 5'-TG-3'-interacting Factor 2) (HRP)

Reactivity

Human

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of SiHa cells using anti- ASS1 antibody (AAA19370).Overlay histogram showing SiHa cells stained with AAA19370 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-ASS1 Antibody (AAA19370, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 7. IF analysis of ASS1 using anti- ASS1 antibody (AAA19370).ASS1 was detected in immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti- ASS1 Antibody (AAA19370) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of ASS1 using anti-ASS1 antibody (AAA19370).ASS1 was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-ASS1 Antibody (AAA19370) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of ASS1 using anti-ASS1 antibody (AAA19370).ASS1 was detected in paraffin-embedded section of human colon cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-ASS1 Antibody (AAA19370) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of ASS1 using anti-ASS1 antibody (AAA19370).ASS1 was detected in paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-ASS1 Antibody (AAA19370) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of ASS1 using anti-ASS1 antibody (AAA19370).ASS1 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-ASS1 Antibody (AAA19370) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of ASS1 using anti-ASS1 antibody (AAA19370).ASS1 was detected in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-ASS1 Antibody (AAA19370) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of ASS1 using anti-ASS1 antibody (AAA19370).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hela whole cell lysatesLane 2: human HEPG2 whole cell lysatesLane 3: human Hek293 wohle cell lysatesLane 4: human THP-1 whole cell lysatesLane 5: human T47D whole cell lysatesLane 6: monkey kidney tissue lysatesLane 7: rat liver tissue lysatesLane 8: rat kidney tissue lysatesLane 9: mouse liver tissue lysatesLane 10: mouse kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti-ASS1 antigen affinity purified monoclonal antibody (Catalog # AAA19370) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for ASS1 at approximately 47KD. The expected band size for ASS1 is at 47KD.)

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ASS1, Monoclonal Antibody (Cat# AAA19370)

Full Name

Anti-ASS1 Antibody (monoclonal, 7I9)

Gene Names

ASS1; ASS; CTLN1

Reactivity

Human, Mouse, Rat, Monkey

Applications

WB, IHC-P, ICC, IF, FC/FACS/FCM

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of MCF-7 cells using anti-DDX1 antibody (AAA19379).Overlay histogram showing MCF-7 cells stained with AAA19379 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- DDX1 Antibody (AAA19379, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of DDX1 using anti-DDX1 antibody (AAA19379).DDX1 was detected in paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-DDX1 Antibody (AAA19379) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of DDX1 using anti-DDX1 antibody (AAA19379).DDX1 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-DDX1 Antibody (AAA19379) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of DDX1 using anti-DDX1 antibody (AAA19379).DDX1 was detected in paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-DDX1 Antibody (AAA19379) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of DDX1 using anti-DDX1 antibody (AAA19379).DDX1 was detected in paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-DDX1 Antibody (AAA19379) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of DDX1 using anti-DDX1 antibody (AAA19379).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human K562 whole cell lysatesLane 2: human CACO-2 whole cell lysatesLane 3: human U20S whole cell lysatesLane 4: human HEK293 whole cell lysatesLane 5: human U87 whole cell lysatesLane 6: human HELA whole cell lysatesLane 7: human A549 whole cell lysatesLane 8: rat kidney tissue lysatesLane 9: mouse kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- DDX1 antigen affinity purified monoclonal antibody (Catalog # AAA19379) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for DDX1 at approximately 86KD. The expected band size for DDX1 is at 86KD.)

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DDX1, Monoclonal Antibody (Cat# AAA19379)

Full Name

Anti-DDX1 Antibody (monoclonal, 2D4)

Gene Names

DDX1; DBP-RB; UKVH5d

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, FC/FACS/FCM

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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WB (Western Blot) (DDX56 monoclonal antibody. Western Blot analysis of DDX56 expression in PC-12.)

WB (Western Blot) (DDX56 monoclonal antibody, Western Blot analysis of DDX56 expression in HeLa.)

Application Data (Detection limit for recombinant GST tagged DDX56 is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to DDX56 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to DDX56 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of DDX56 expression in transfected 293T cell line by DDX56 monoclonal antibody. Lane 1: DDX56 transfected lysate (62kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (36.89kD).)

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DDX56, Monoclonal Antibody (Cat# AAA24189)

Full Name

DDX56 (Probable ATP-dependent RNA Helicase DDX56, ATP-dependent 61kD Nucleolar RNA Helicase, DEAD Box Protein 21, DEAD Box Protein 56, DDX21, NOH61) (AP)

Reactivity

Human, Rat

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (DDX54 monoclonal antibody, Western Blot analysis of DDX54 expression in HeLa.)

Application Data (Detection limit for recombinant GST tagged DDX54 is 0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to DDX54 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to DDX54 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of DDX54 expression in transfected 293T cell line by DDX54 monoclonal antibody. Lane 1: DDX54 transfected lysate (98.6kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (37.55kD).)

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DDX54, Monoclonal Antibody (Cat# AAA24188)

Full Name

DDX54 (ATP-dependent RNA Helicase DDX54, ATP-dependent RNA Helicase DP97, DEAD Box RNA Helicase 97kD, DEAD Box Protein 54, MGC2835) (AP)

Gene Names

DDX54; DP97

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (LDB3 monoclonal antibody Western Blot analysis of LDB3 expression in A-431.)

WB (Western Blot) (Western blot analysis of LDB3 over-expressed 293 cell line, cotransfected with LDB3 Validated Chimera RNAi ((Lane 2) or non-transfected control (Lane 1). Blot probed with LDB3 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged LDB3 is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to LDB3 on A-431 cell. [antibody concentration 10ug/ml])

WB (Western Blot) (Western Blot analysis of LDB3 expression in transfected 293T cell line by LDB3 monoclonal antibody. Lane 1: LDB3 transfected lysate (31kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (57.24kD).)

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LDB3, Monoclonal Antibody (Cat# AAA24255)

Full Name

LDB3 (LIM Domain-binding Protein 3, Protein Cypher, Z-band Alternatively Spliced PDZ-motif Protein, KIAA0613, ZASP) (AP)

Gene Names

LDB3; MFM4; ZASP; CMD1C; CMH24; CMPD3; LVNC3; CYPHER; LDB3Z1; LDB3Z4; ORACLE; PDLIM6

Reactivity

Human

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (SCARA3 monoclonal antibody, Western Blot analysis of SCARA3 expression in IMR-32.)

Application Data (Detection limit for recombinant GST tagged SCARA3 is ~3ng/ml as a capture antibody.)

WB (Western Blot) (SCARA3 monoclonal antibody, Western Blot analysis of SCARA3 expression in NIH/3T3.)

WB (Western Blot) (SCARA3 monoclonal antibody. Western Blot analysis of SCARA3 expression in HepG2.)

WB (Western Blot) (SCARA3 monoclonal antibody. Western Blot analysis of SCARA3 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)

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SCARA3, Monoclonal Antibody (Cat# AAA25536)

Full Name

SCARA3 (Scavenger Receptor Class A Member 3, Cellular Stress Response Gene Protein , CSR, CSR1, APC7, MSLR1, MSRL1) (HRP)

Gene Names

SCARA3; CSR; APC7; CSR1; MSLR1; MSRL1

Reactivity

Human, Mouse, Rat

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HDAC1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of HDAC1 expression in transfected 293T cell line by HDAC1 monoclonal antibody Lane 1: HDAC1 transfected lysate (55.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (HDAC1 monoclonal antibody Western Blot analysis of HDAC1 expression in NIH/3T3)

WB (Western Blot) (HDAC1 monoclonal antibody Western Blot analysis of HDAC1 expression in Raw 264.7)

WB (Western Blot) (HDAC1 monoclonal antibody Western Blot analysis of HDAC1 expression in Hela NE)

WB (Western Blot) (HDAC1 monoclonal antibody. Western Blot analysis of HDAC1 expression in PC-12)

WB (Western Blot) (Western Blot detection against Immunogen (78.76kD).)

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HDAC1, Monoclonal Antibody (Cat# AAA24235)

Full Name

HDAC1 (Histone Deacetylase 1, HD1, HD-1, HDAC1, HDAC-1, RPD3, RPD3L1, Reduced Potassium Dependency 3 Like-1) (AP)

Gene Names

HDAC1; HD1; RPD3; GON-10; RPD3L1

Reactivity

Human, Mouse, Rat

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (ACBD3 monoclonal antibody. Western Blot analysis of ACBD3 expression in PC-12.)

WB (Western Blot) (ACBD3 monoclonal antibody, Western Blot analysis of ACBD3 expression in HeLa.)

Application Data (Detection limit for recombinant GST tagged ACBD3 is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ACBD3 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ACBD3 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of ACBD3 expression in transfected 293T cell line by ACBD3 monoclonal antibody. Lane 1: ACBD3 transfected lysate (60.6kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (37kD).)

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ACBD3, Monoclonal Antibody (Cat# AAA25300)

Full Name

ACBD3 (Golgi Resident Protein GCP60, Acyl-CoA-binding Domain-containing Protein 3, Golgi Complex-associated Protein 1, GOCAP1, Golgi Phosphoprotein 1, GOLPH1, PBR- and PKA-associated Protein 7, Peripheral Benzodiazepine Receptor-associated Protein PAP7, G

Gene Names

ACBD3; PAP7; GCP60; GOCAP1; GOLPH1

Reactivity

Human, Mouse, Rat

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (CDADC1 monoclonal antibody. Western Blot analysis of CDADC1 expression in NIH/3T3.)

WB (Western Blot) (CDADC1 monoclonal antibody. Western Blot analysis of CDADC1 expression in Raw 264.7.)

WB (Western Blot) (Western blot analysis of CDADC1 over-expressed 293 cell line, cotransfected with CDADC1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CDADC1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged CDADC1 is ~0.03ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CDADC1 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1ug/ml].)

WB (Western Blot) (Western Blot analysis of CDADC1 expression in transfected 293T cell line by CDADC1 monoclonal antibody. Lane 1: CDADC1 transfected lysate (58.5kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (36.23kD).)

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CDADC1, Monoclonal Antibody (Cat# AAA25049)

Full Name

CDADC1 (Cytidine and dCMP Deaminase Domain-containing Protein 1, Testis Development Protein NYD-SP15, MGC150615, MGC41774, MGC57136, NYD-SP15, BA103J18.1) (FITC)

Gene Names

CDADC1; NYD-SP15; bA103J18.1

Reactivity

Human, Mouse

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western blot analysis of SP110 over-expressed 293 cell line, cotransfected with SP110 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with SP110 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged SP110 is ~0.1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of SP110 transfected lysate using SP110 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with SP110 rabbit polyclonal antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SP110 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of SP110 expression in transfected 293T cell line by SP110 monoclonal antibody. Lane 1: SP110 transfected lysate (61.8kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (SP110 monoclonal antibody. Western Blot analysis of SP110 expression in Jurkat.)

WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)

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IPR1, Monoclonal Antibody (Cat# AAA24543)

Full Name

IPR1 (SP110, Sp110 Nuclear Body Protein, Interferon-induced Protein 41/75, Speckled 110kD, Transcriptional Coactivator Sp110) APC

Gene Names

SP110; IPR1; VODI; IFI41; IFI75

Reactivity

Human

Applications

EIA, IHC, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 300ug extracts of HeLa cells using 3ug SQSTM1/p62 antibody . Western blot was performed from the immunoprecipitate using SQSTM1/p62 antibody at a dilition of 1:1000.)

IF (Immunofluorescence) (Immunofluorescence analysis of L929 cells using SQSTM1/p62 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using SQSTM1/p62 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using SQSTM1/p62 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat spleen using SQSTM1/p62 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using p62/SQSTM1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)

WB (Western Blot) (Western blot analysis of extracts from normal (control) and SQSTM1/p62 knockout (KO) 293T cells, using SQSTM1/p62 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 3min.)

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SQSTM1/p62, Monoclonal Antibody (Cat# AAA28383)

Full Name

[KO Validated] SQSTM1/p62 Rabbit mAb

Gene Names

SQSTM1; p60; p62; A170; OSIL; PDB3; ZIP3; p62B

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, IP

Purity

Affinity purification

Pricing

$300/0.02 mL $500/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,780/4x0.1 mL $1,960/5x0.1 mL

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WB (Western Blot) (Western blot analysis of GABBR1 over-expressed 293 cell line, cotransfected with GABBR1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with GABBR1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged GABBR1 is ~0.3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GABBR1 on HeLa cell. [antibody concentration 10ug/ml].)

WB (Western Blot) (Western Blot analysis of GABBR1 expression in transfected 293T cell line by GABBR1 monoclonal antibody. Lane 1: GABBR1 transfected lysate (95kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (GABBR1 monoclonal antibody, Western Blot analysis of GABBR1 expression in IMR-32.)

WB (Western Blot) (Western Blot detection against Immunogen (36.63kD).)

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GABBR1, Monoclonal Antibody (Cat# AAA24810)

Full Name

GABBR1 (Gamma-aminobutyric Acid Type B Receptor Subunit 1, GABA-B Receptor 1, GABA-B-R1, GABA-BR1, GABABR1, Gb1) (Biotin)

Gene Names

GABBR1; GB1; GPRC3A; GABABR1; GABBR1-3

Reactivity

Human

Applications

EIA, IF, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Dilution: WB 1:500-2000,IHC-p 1:50-300)

Application Data (Dilution: WB 1:500-2000,IHC-p 1:50-300)

Application Data (Dilution: WB 1:500-2000,IHC-p 1:50-300)

Application Data (Dilution: WB 1:500-2000,IHC-p 1:50-300)

Application Data (Dilution: WB 1:500-2000,IHC-p 1:50-300)

WB (Western Blot) (Dilution: WB 1:500-2000,IHC-p 1:50-300)

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HP-1gamma, Monoclonal Antibody (Cat# AAA28914)

Full Name

HP-1gamma mouse Monoclonal Antibody (3B9)

Gene Names

CBX3; HECH; HP1-GAMMA; HP1Hs-gamma

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 IHC-p 1:50-300)

IHC (Immunohistchemistry) (Dilution: IF: 1:50-200 IHC-p 1:50-300)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 IHC-p 1:50-300)

Application Data (Dilution: IF: 1:50-200 IHC-p 1:50-300)

IHC (Immunohistochemistry-Paraffin) (Dilution: IF: 1:50-200 IHC-p 1:50-300)

IHC (Immunohistochemistry-Paraffin) (Dilution: IF: 1:50-200 IHC-p 1:50-300)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 IHC-p 1:50-300)

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NBR1, Monoclonal Antibody (Cat# AAA28916)

Full Name

NBR1 mouse Monoclonal Antibody (7C3)

Gene Names

NBR1; IAI3B; M17S2; MIG19; 1A1-3B

Reactivity

Human, Mouse, Rat

Applications

IHC-P, IF

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of THP-1 cells using anti-Ku70 antibody (AAA19361).Overlay histogram showing THP-1 cells stained with AAA19361 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- Ku70 Antibody (AAA19361, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 6. IF analysis of Ku70 using anti- Ku70 antibody (AAA19361).Ku70 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti- Ku70 Antibody (AAA19361) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of Ku70 using anti-Ku70 antibody (AAA19361).Ku70 was detected in paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Ku70 Antibody (AAA19361) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of Ku70 using anti-Ku70 antibody (AAA19361).Ku70 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Ku70 Antibody (AAA19361) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of Ku70 using anti-Ku70 antibody (AAA19361).Ku70 was detected in paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Ku70 Antibody (AAA19361) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of Ku70 using anti-Ku70 antibody (AAA19361).Ku70 was detected in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Ku70 Antibody (AAA19361) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of Ku70 using anti-Ku70 antibody (AAA19361).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human Hela whole cell lysatesLane 2: human A549 whole cell lysatesLane 3: human HepG2 whole cell lysatesLane 4: human MCF-7 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- Ku70 antigen affinity purified monoclonal antibody (Catalog # AAA19361) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for Ku70 at approximately 70KD. The expected band size for Ku70 is at 70KD.)

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Ku70, Monoclonal Antibody (Cat# AAA19361)

Full Name

Anti-Ku70 Antibody (monoclonal, 9B6)

Gene Names

XRCC6; ML8; KU70; TLAA; CTC75; CTCBF; G22P1

Reactivity

Human

Applications

WB, IHC-P, ICC, IF, FC/FACS/FCM

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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FCM (Flow Cytometry) (Figure 12. Flow Cytometry analysis of U251 cells using anti-HP1 alpha/CBX5 antibody (AAA19373).Overlay histogram showing U251 cells stained with AAA19373 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- HP1 alpha/CBX5 Antibody (AAA19373, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 11. IF analysis of HP1 alpha/CBX5 using anti- HP1 alpha/CBX5 antibody (AAA19373).HP1 alpha/CBX5 was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti- HP1 alpha/CBX5 Antibody (AAA19373) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 10. IHC analysis of HP1 alpha/CBX5 using anti-HP1 alpha/CBX5 antibody (AAA19373).HP1 alpha/CBX5 was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-HP1 alpha/CBX5 Antibody (AAA19373) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 9. IHC analysis of HP1 alpha/CBX5 using anti-HP1 alpha/CBX5 antibody (AAA19373).HP1 alpha/CBX5 was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-HP1 alpha/CBX5 Antibody (AAA19373) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 8. IHC analysis of HP1 alpha/CBX5 using anti-HP1 alpha/CBX5 antibody (AAA19373).HP1 alpha/CBX5 was detected in paraffin-embedded section of human renal clear cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-HP1 alpha/CBX5 Antibody (AAA19373) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of HP1 alpha/CBX5 using anti-HP1 alpha/CBX5 antibody (AAA19373).HP1 alpha/CBX5 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-HP1 alpha/CBX5 Antibody (AAA19373) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of HP1 alpha/CBX5 using anti-HP1 alpha/CBX5 antibody (AAA19373).HP1 alpha/CBX5 was detected in paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-HP1 alpha/CBX5 Antibody (AAA19373) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of HP1 alpha/CBX5 using anti-HP1 alpha/CBX5 antibody (AAA19373).HP1 alpha/CBX5 was detected in paraffin-embedded section of human gallbladder adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-HP1 alpha/CBX5 Antibody (AAA19373) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of HP1 alpha/CBX5 using anti-HP1 alpha/CBX5 antibody (AAA19373).HP1 alpha/CBX5 was detected in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-HP1 alpha/CBX5 Antibody (AAA19373) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of HP1 alpha/CBX5 using anti-HP1 alpha/CBX5 antibody (AAA19373).HP1 alpha/CBX5 was detected in paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-HP1 alpha/CBX5 Antibody (AAA19373) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of HP1 alpha/CBX5 using anti-HP1 alpha/CBX5 antibody (AAA19373).HP1 alpha/CBX5 was detected in paraffin-embedded section of human adrenocortical adenoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-HP1 alpha/CBX5 Antibody (AAA19373) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of HP1 alpha/CBX5 using anti-HP1 alpha/CBX5 antibody (AAA19373).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human U87 whole cell lysatesLane 2: human K562 whole cell lysatesLane 3: human Jurkat whole cell lysatesLane 4: rat brain tissue lysatesLane 5: rat PC-12 whole cell lysatesLane 6: mouse brain tissue lysatesLane 7: mouse SP2/0 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- HP1 alpha/CBX5 antigen affinity purified monoclonal antibody (Catalog # AAA19373) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for HP1 alpha/CBX5 at approximately 22KD. The expected band size for HP1 alpha/CBX5 is at 22KD.)

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HP1 alpha/CBX5, Monoclonal Antibody (Cat# AAA19373)

Full Name

Anti-HP1 alpha/CBX5 Antibody (monoclonal, 8G6)

Gene Names

CBX5; HP1; HP1A

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, ICC, IF, FC/FACS/FCM

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of U937 cells using anti-Glutaminase/GLS antibody (AAA19683).Overlay histogram showing U937 cells stained with AAA19683 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Glutaminase/GLS Antibody (AAA19683, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 7. IF analysis of Glutaminase/GLS using anti-Glutaminase/GLS antibody (AAA19683).Glutaminase/GLS was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL mouse anti-Glutaminase/GLS Antibody (AAA19683) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of Glutaminase/GLS using anti-Glutaminase/GLS antibody (AAA19683).Glutaminase/GLS was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Glutaminase/GLS Antibody (AAA19683) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of Glutaminase/GLS using anti-Glutaminase/GLS antibody (AAA19683).Glutaminase/GLS was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Glutaminase/GLS Antibody (AAA19683) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of Glutaminase/GLS using anti-Glutaminase/GLS antibody (AAA19683).Glutaminase/GLS was detected in a paraffin-embedded section of human appendiceal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Glutaminase/GLS Antibody (AAA19683) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of Glutaminase/GLS using anti-Glutaminase/GLS antibody (AAA19683).Glutaminase/GLS was detected in a paraffin-embedded section of human gall bladder adenosquamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Glutaminase/GLS Antibody (AAA19683) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

WB (Western Blot) (Figure 2. Western blot analysis of Glutaminase/GLS using anti-Glutaminase/GLS antibody (AAA19683).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: rat brain tissue lysates,Lane 2: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Glutaminase/GLS antigen affinity purified monoclonal antibody (#AAA19683) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Glutaminase/GLS at approximately 56-73 kDa. The expected band size for Glutaminase/GLS is at 73 kDa.)

WB (Western Blot) (Figure 1. Western blot analysis of Glutaminase/GLS using anti-Glutaminase/GLS antibody (AAA19683).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human HepG2 whole cell lysates,Lane 2: human HEL whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Glutaminase/GLS antigen affinity purified monoclonal antibody (#AAA19683) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Glutaminase/GLS at approximately 56-73 kDa. The expected band size for Glutaminase/GLS is at 73 kDa.)

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Glutaminase/GLS, Monoclonal Antibody (Cat# AAA19683)

Full Name

Anti-Glutaminase/GLS Antibody Picoband (monoclonal, 3G13)

Gene Names

GLS; GAC; GAM; KGA; GLS1; AAD20

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ICC, IF, FC/FACS

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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Application Data

Application Data

Application Data (Detection limit for 123091 is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence to AHR on HeLa cell using 123091 (10ug/ml).)

IP (Immunoprecipitation) (Immunoprecipitation of AHR transfected lysate using 123091 and Protein A Magnetic Bead and immunoblotted with AHR rabbit polyclonal antibody.)

WB (Western Blot) (Western Blot analysis of AHR over-expressed 293 cell line, cotransfected with AHR Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with 123091. GAPDH (36.1kD) used as specificity and loading control.)

WB (Western Blot) (Western Blot detection against immunogen (36.74kD).)

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AHR, Monoclonal Antibody (Cat# AAA24719)

Full Name

AHR (Aryl Hydrocarbon Receptor, Ah Receptor, AhR, Class E Basic Helix-loop-helix Protein 76, bHLHe76, BHLHE76) (Biotin)

Gene Names

AHR; bHLHe76

Reactivity

Human

Applications

EIA, IF, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (CAPNS1 monoclonal antibody, Western Blot analysis of CAPNS1 expression in A-431.)

WB (Western Blot) (Western blot analysis of CAPNS1 over-expressed 293 cell line, cotransfected with CAPNS1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CAPNS1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged CAPNS1 is ~0.1ng/ml when antibody 124328 was used as capture antibody.)

IF (Immunofluorescence) (Immunofluorescence staining of CAPNS1 on HeLa cells using antibody 124328 at 20ug/ml.)

IHC (Immunohistochemistry) (Immunoperoxidase staining of CAPNS1 on formalin-fixed paraffin-embedded human kidney using antibody 124328 at 3ug/ml.)

WB (Western Blot) (Western Blot analysis of CAPNS1 expression in transfected 293T cell line using antibody 124328: Lane 1: CAPNS1 transfected lysate (28.3kD) Lane 2: Non-transfected lysate)

WB (Western Blot) (Western Blot analysis of CAPNS1 expression in PC-12 cells using antibody 124328.)

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CAPNS1, Monoclonal Antibody (Cat# AAA24746)

Full Name

CAPNS1 (Calpain Small Subunit 1, CSS1, Calcium-activated Neutral Proteinase Small Subunit, CANP Small Subunit, Calcium-dependent Protease Small Subunit, CDPS, Calcium-dependent Protease Small Subunit 1, Calpain Regulatory Subunit, CAPN4, CAPNS) (Biotin)

Gene Names

CAPNS1; CANP; CDPS; CSS1; CANPS; CAPN4; CALPAIN4

Reactivity

Human, Rat

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (CDC2 monoclonal antibody (M04), clone 8F1. Western Blot analysis of CDC2 expression in PC-12.)

WB (Western Blot) (CDC2 monoclonal antibody (M04), clone 8F1. Western Blot analysis of CDC2 expression in NIH/3T3.)

Application Data (Detection limit for recombinant GST tagged CDC2 is approximately 0.1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CDC2 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 1.5 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CDC2 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 1.5 ug/ml])

WB (Western Blot) (CDC2 monoclonal antibody (M04), clone 8F1 Western Blot analysis of CDC2 expression in Hela S3 NE.)

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CDC2, Monoclonal Antibody (Cat# AAA26021)

Full Name

CDC2 (Cell Division Cycle 2, G1 to S and G2 to M, CDC28A, CDK1, DKFZp686L20222, MGC111195) (APC)

Gene Names

CDK1; CDC2; CDC28A; P34CDC2

Applications

IHC, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged NDUFA9 is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NDUFA9 on NIH/3T3 cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NDUFA9 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 0.8ug/ml])

WB (Western Blot) (NDUFA9 monoclonal antibody Western Blot analysis of NDUFA9 expression in NIH/3T3.)

WB (Western Blot) (NDUFA9 monoclonal antibody. Western Blot analysis of NDUFA9 expression in Raw 264.7.)

WB (Western Blot) (NDUFA9 monoclonal antibody Western Blot analysis of NDUFA9 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (33.99kD).)

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NDUFA9, Monoclonal Antibody (Cat# AAA25762)

Full Name

NDUFA9 (NADH Dehydrogenase [Ubiquinone] 1 alpha Subcomplex Subunit 9, Mitochondrial, NADH-Ubiquinone Oxidoreductase 39kD Subunit, Complex I-39kD, CI-39kD, NDUFS2L, MGC111043) (PE)

Gene Names

NDUFA9; CC6; CI39k; CI-39k; MC1DN26; NDUFS2L; SDR22E1

Reactivity

Human, Mouse, Rat

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between AKT1 and HSPB1. Mahlavu cells were stained with AKT1 rabbit purified polyclonal 1:1200 and HSPB1 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Application Data (Detection limit for recombinant GST tagged HSPB1 is ~0.1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of HSPB1 transfected lysate using HSPB1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HSPB1 rabbit polyclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HSPB1 on HeLa cell. [antibody concentration 10ug/ml])

WB (Western Blot) (Western Blot analysis of HSPB1 expression in transfected 293T cell line by HSPB1 monoclonal antibody. Lane 1: HSPB1 transfected lysate (22.8kD). Lane 2: Non-transfected lysate.)

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HSPB1, Monoclonal Antibody (Cat# AAA24243)

Full Name

HSPB1 (Heat Shock Protein beta-1, HspB1, Heat Shock 27kD Protein, HSP 27, Stress-responsive Protein 27, SRP27, Estrogen-regulated 24kD Protein, 28kD Heat Shock Protein, HSPB1, HSP27) (AP)

Gene Names

HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102

Reactivity

Human

Applications

EIA, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western blot analysis of SARS over-expressed 293 cell line, cotransfected with SARS Validated Chimera RNAi (301-R01V) (Lane 2) or non-transfected control (Lane 1). Blot probed with SARS monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged SARS is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SARS on HeLa cell. [antibody concentration 25ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SARS on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of SARS expression in transfected 293T cell line by SARS monoclonal antibody. Lane 1: SARS transfected lysate (58.8kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (SARS monoclonal antibody, Western Blot analysis of SARS expression in Jurkat.)

WB (Western Blot) (Western Blot detection against Immunogen (82.28kD).)

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SARS, Monoclonal Antibody (Cat# AAA25534)

Full Name

SARS (Seryl-tRNA Synthetase, Cytoplasmic, Seryl-tRNA(Ser/Sec) Synthetase, Serine-tRNA Ligase, SerRS, SERS, FLJ36399) (HRP)

Gene Names

SARS; SERS; SERRS

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

What are Monoclonal Antibodies?

Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.

This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.

AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.

What Are The Uses of Monoclonal Antibodies

Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.

ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.

Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.

Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.

Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.

How Monoclonal Antibodies Are Used as Medicine?

Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).

Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:

• Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.


• Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.


• Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.

Why Buy Monoclonal Antibodies From Us?

At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.

1. Validated for Versatile Applications

The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).

2. Wide Selection & Specialized Options

We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.

3. High-Quality Proteins

Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.

Frequently Asked Questions

1. Are your monoclonal antibodies validated for specific applications?

Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.

2. How do I choose the right monoclonal antibody for my application?

Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.

3. How quickly can I receive my order?

Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.