Monoclonal Antibodies

Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.

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IF (Immunofluorescence) (Figure 13. IF analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (AAA19702).PC4/SUB1 was detected in a paraffin-embedded section of human intestine cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 ug/mL mouse anti-PC4/SUB1 Antibody (AAA19702) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

FCM (Flow Cytometry) (Figure 12. Flow Cytometry analysis of HepG2 cells using anti-PC4/SUB1 antibody (AAA19702).Overlay histogram showing HepG2 cells stained with AAA19702 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-PC4/SUB1 Antibody (AAA19702, 1 ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG (BA1126, 5-10 ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 11. IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (AAA19702).PC4/SUB1 was detected in a paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-PC4/SUB1 Antibody (AAA19702) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 10. IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (AAA19702).PC4/SUB1 was detected in a paraffin-embedded section of mouse liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-PC4/SUB1 Antibody (AAA19702) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 9. IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (AAA19702).PC4/SUB1 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-PC4/SUB1 Antibody (AAA19702) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 8. IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (AAA19702).PC4/SUB1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-PC4/SUB1 Antibody (AAA19702) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (AAA19702).PC4/SUB1 was detected in a paraffin-embedded section of human testicular germ cell tumor tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-PC4/SUB1 Antibody (AAA19702) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (AAA19702).PC4/SUB1 was detected in a paraffin-embedded section of human hepatocellular carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-PC4/SUB1 Antibody (AAA19702) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (AAA19702).PC4/SUB1 was detected in a paraffin-embedded section of human hepatocellular carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-PC4/SUB1 Antibody (AAA19702) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (AAA19702).PC4/SUB1 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-PC4/SUB1 Antibody (AAA19702) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (AAA19702).PC4/SUB1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-PC4/SUB1 Antibody (AAA19702) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (AAA19702).PC4/SUB1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-PC4/SUB1 Antibody (AAA19702) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of PC4/SUB1 using anti-PC4/SUB1 antibody (AAA19702).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human A549 whole cell lysates,Lane 3: human MCF-7 whole cell lysates,Lane 4: human T47D whole cell lysates,Lane 5: rat liver tissue lysates,Lane 6: rat spleen tissue lysates,Lane 7: mouse spleen tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-PC4/SUB1 antigen affinity purified monoclonal antibody (#AAA19702) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PC4/SUB1 at approximately 19 kDa. The expected band size for PC4/SUB1 is at 14 kDa.)

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PC4/SUB1, Monoclonal Antibody (Cat# AAA19702)

Full Name

Anti-PC4/SUB1 Antibody Picoband (monoclonal, 8D9D1)

Gene Names

SUB1; P15; PC4; p14

Reactivity

Human, Mouse, Rat

Applications

FC/FACS, IF, IHC, WB

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of Jurkat cells using anti-Ki67 antibody (AAA19663).Overlay histogram showing Jurkat cells stained with AAA19663 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Ki67 Antibody (AAA19663, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 7. IF analysis of Ki67 using anti-Ki67 antibody (AAA19663).Ki67 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL mouse anti-Ki67 Antibody (AAA19663) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 6. IHC analysis of Ki67 using anti-Ki67 antibody (AAA19663).Ki67 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Ki67 Antibody (AAA19663) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of Ki67 using anti-Ki67 antibody (AAA19663).Ki67 was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Ki67 Antibody (AAA19663) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of Ki67 using anti-Ki67 antibody (AAA19663).Ki67 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Ki67 Antibody (AAA19663) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of Ki67 using anti-Ki67 antibody (AAA19663).Ki67 was detected in a paraffin-embedded section of human lymphomas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Ki67 Antibody (AAA19663) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of Ki67 using anti-Ki67 antibody (AAA19663).Ki67 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Ki67 Antibody (AAA19663) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

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Ki67, Monoclonal Antibody (Cat# AAA19663)

Full Name

Anti-Ki67 Antibody Picoband (monoclonal, 5C7)

Gene Names

MKI67; KIA

Reactivity

Human, Mouse

Applications

WB, IHC, ICC, IF, FC/FACS

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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Application Data (Detection limit for recombinant GST tagged SMAD3 is approximately 3ng/ml as a capture antibody.)

WB (Western Blot) (SMAD3 monoclonal antibody (M09), clone 1C11. Western Blot analysis of SMAD3 expression in SJCRH30 (Cat # L027V1).)

WB (Western Blot) (SMAD3 monoclonal antibody (M09), clone 1C11. Western Blot analysis of SMAD3 expression in Jurkat (Cat # L017V1).)

WB (Western Blot) (SMAD3 monoclonal antibody (M09), clone 1C11 Western Blot analysis of SMAD3 expression in Hela S3 NE (Cat # L013V3).)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SMAD3 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SMAD3 on HeLa cell. [antibody concentration 10 ug/ml])

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SMAD3, Monoclonal Antibody (Cat# AAA26297)

Full Name

SMAD3 (SMAD Family Member 3, DKFZp586N0721, DKFZp686J10186, HSPC193, HsT17436, JV15-2, MADH3, MGC60396) (FITC)

Gene Names

SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436

Applications

IF, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged PGGT1B is ~1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PGGT1B on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PGGT1B on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1.2ug/ml])

WB (Western Blot) (PGGT1B monoclonal antibody Western Blot analysis of PGGT1B expression in PC-12.)

WB (Western Blot) (PGGT1B monoclonal antibody Western Blot analysis of PGGT1B expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (37.29kD).)

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PGGT1B, Monoclonal Antibody (Cat# AAA14773)

Full Name

PGGT1B (Geranylgeranyl Transferase Type-1 Subunit beta, Geranylgeranyl Transferase Type I Subunit beta, GGTase-I-beta, Type I Protein Geranyl-Geranyltransferase Subunit beta)

Gene Names

Pggt1b; BGG1; GGT1; AI451237; AI551093; 2610100E13; 2010207C17Rik

Reactivity

Human, Rat

Applications

EL/EIA, WB, IHC, IF

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

$820/0.1 mg $1,565/2x0.1 mg $2,270/3x0.1 mg $2,940/4x0.1 mg $3,380/5x0.1 mg

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WB (Western Blot) (MTA1 monoclonal antibody (M02), clone 4D11. Western Blot analysis of MTA1 expression in PC-12 (Cat # L012V1).)

Application Data (Detection limit for recombinant GST tagged MTA1 is approximately 3ng/ml as a capture antibody.)

WB (Western Blot) (MTA1 monoclonal antibody (M02), clone 4D11. Western Blot analysis of MTA1 expression in NIH/3T3 (Cat # L018V1).)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MTA1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MTA1 on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (MTA1 monoclonal antibody (M02), clone 4D11 Western Blot analysis of MTA1 expression in Hela S3 NE (Cat # L013V3).)

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MTA1, Monoclonal Antibody (Cat# AAA26311)

Full Name

MTA1 (Metastasis Associated 1) (FITC)

Applications

IF, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged HMGB2 is approximately 0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HMGB2 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HMGB2 on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (HMGB2 monoclonal antibody (M04), clone 3D2 Western Blot analysis of HMGB2 expression in Hela S3 NE.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HMGB2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to HMGB2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])

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HMGB2, Monoclonal Antibody (Cat# AAA26092)

Full Name

HMGB2 (High-Mobility Group Box 2, HMG2) (APC)

Applications

IF, IHC, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of Caco-2 cells using anti-HGS antibody (AAA19678).Overlay histogram showing Caco-2 cells stained with AAA19678 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-HGS Antibody (AAA19678, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 8. IHC analysis of HGS using anti-HGS antibody (AAA19678).HGS was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-HGS Antibody (AAA19678) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of HGS using anti-HGS antibody (AAA19678).HGS was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-HGS Antibody (AAA19678) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of HGS using anti-HGS antibody (AAA19678).HGS was detected in a paraffin-embedded section of human breast infiltrating ductal carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-HGS Antibody (AAA19678) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of HGS using anti-HGS antibody (AAA19678).HGS was detected in a paraffin-embedded section of human hepatocellular carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-HGS Antibody (AAA19678) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of HGS using anti-HGS antibody (AAA19678).HGS was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-HGS Antibody (AAA19678) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of HGS using anti-HGS antibody (AAA19678).HGS was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-HGS Antibody (AAA19678) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of HGS using anti-HGS antibody (AAA19678).HGS was detected in a paraffin-embedded section of human laryngeal squamous cell carcinomas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-HGS Antibody (AAA19678) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of HGS using anti-HGS antibody (M00179-1).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human 293T whole cell lysates,Lane 3: human K562 whole cell lysates,Lane 4: rat brain tissue lysates,Lane 5: rat PC-12 whole cell lysates,Lane 6: mouse brain tissue lysates,Lane 7: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HGS antigen affinity purified monoclonal antibody (#M00179-1) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HGS at approximately 110 kDa. The expected band size for HGS is at 110 kDa.)

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HGS, Monoclonal Antibody (Cat# AAA19678)

Full Name

Anti-HGS Antibody Picoband (monoclonal, 6C2E1)

Gene Names

HGS; HRS

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, FC/FACS

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of JK cells using anti-RPA32/RPA2 antibody (AAA19693).Overlay histogram showing JK cells stained with AAA19693 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-RPA32/RPA2 Antibody (AAA19693, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 6. IF analysis of RPA32/RPA2 using anti-RPA32/RPA2 antibody (AAA19693).RPA32/RPA2 was detected in an immunocytochemical section of HeLa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL mouse anti-RPA32/RPA2 Antibody (AAA19693) overnight at 4 degree C. DyLight594 Conjugated Goat Anti-Mouse IgG (BA1141) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The tissue section was developed using Phalloidin-iFluor 488 Conjugated. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of RPA32/RPA2 using anti-RPA32/RPA2 antibody (AAA19693).RPA32/RPA2 was detected in a paraffin-embedded section of human colonic adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-RPA32/RPA2 Antibody (AAA19693) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of RPA32/RPA2 using anti-RPA32/RPA2 antibody (AAA19693).RPA32/RPA2 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-RPA32/RPA2 Antibody (AAA19693) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of RPA32/RPA2 using anti-RPA32/RPA2 antibody (AAA19693).RPA32/RPA2 was detected in a paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-RPA32/RPA2 Antibody (AAA19693) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of RPA32/RPA2 using anti-RPA32/RPA2 antibody (AAA19693).RPA32/RPA2 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-RPA32/RPA2 Antibody (AAA19693) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of RPA32/RPA2 using anti-RPA32/RPA2 antibody (AAA19693).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human U20S whole cell lysates,Lane 2: human HEK293 whole cell lysates,Lane 3: human Jurkat whole cell lysates,Lane 4: human K562 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-RPA32/RPA2 antigen affinity purified monoclonal antibody (#AAA19693) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RPA32/RPA2 at approximately 32 kDa. The expected band size for RPA32/RPA2 is at 32 kDa.)

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RPA32/RPA2, Monoclonal Antibody (Cat# AAA19693)

Full Name

Anti-RPA32/RPA2 Antibody Picoband (monoclonal, 3B2E9)

Gene Names

RPA2; REPA2; RPA32

Reactivity

Human

Applications

WB, IHC, ICC, IF, FC/FACS

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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WB (Western Blot) (SSH3 monoclonal antibody, Western Blot analysis of SSH3 expression in A-431.)

Application Data (Detection limit for recombinant GST tagged SSH3 is ~0.3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SSH3 on A-431 cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SSH3 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 1-10ug/ml])

WB (Western Blot) (Western Blot analysis of SSH3 expression in transfected 293T cell line by SSH3 monoclonal antibody. Lane 1: SSH3 transfected lysate (73kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (37kD).)

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SSH3, Monoclonal Antibody (Cat# AAA25854)

Full Name

SSH3 (Protein Phosphatase Slingshot Homolog 3, SSH-like Protein 3, SSH3L, SSH-3L, hSSH-3L) (PE)

Gene Names

SSH3; SSH3L

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IP (Immunoprecipitation) (Figure 6. Immunoprecipitation analysis. Cellular overexpression lysates (made from HEK293F cells transfected with DYKDDDDK tagged human BCMA full length gene) were pre-incubated with 6 different rabbit DimAb clones and negative control IgG. The immunocomplexes were further pulled down by protein A beads, fractionated, and blotted with mouse anti-DYKDDDDK monoclonal antibody.)

ELISA (Figure 5. ELISA plate was coated with recombinant BCMA-hFc fusion protein (PME100001), followed by pre-blocking with huC11D5.3 antibody (Grey bar) or rabbit control IgG (Black bar), and then different rabbit DimAbs antibodies were added to check the competitive inhibition of huC11D5.3. DM3 clone exhibits the strongest inhibition (Red bar). This data indicated that DM3 bind to the same epitope as bb2121.)

Application Data (Figure 4. Affinity ranking of different DimAb clones by titration of rabbit DimAb antibody concentration onto K562-BCMA or NCI-H929 cells. Different concentrations of various anti-BCMA DimAb clones were incubated with K562-BCMA (A) or NCI-H929 cells (B) at 4 degree C. Bound rabbit IgG was detected in flow cytometry analysis. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)

Application Data (Figure 3. Phylogenetic analysis of different Anti-BCMA DimAb clones. A) heavy chain and B) Light chain.)

Application Data

FCM (Flow Cytometry) (Figure 2. A. Flow cytometry analysis with anti-BCMA (DM6) on NCI-H929 cells (Red histogram) or rabbit control antibody on NCI-H929 cells (Blue histogram). B. Flow cytometry data of serially titrated anti-BCMA (DM6) on NCI-H929 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)

Application Data

FCM (Flow Cytometry) (Figure 1. A. Flow cytometry analysis with anti-BCMA (DM6) on K562-BCMA (Red histogram)(K562 cells stably transduced by human BCMA full length gene) and K562 (Negative control cell line)(Blue histogram). B. Flow cytometry data of serially titrated anti-BCMA (DM6). The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)

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BCMA, Monoclonal Antibody (Cat# AAA30719)

Full Name

Anti-BCMA antibody (DM6), Rabbit mAb

Reactivity

Human

Applications

EIA, FC/FACS, IF

Purity

Purified from cell culture supernatant by affinity chromatography

Pricing

$250/0.01 mg $890/0.1 mg $1,705/2x0.1 mg $2,480/3x0.1 mg $3,210/4x0.1 mg $3,710/5x0.1 mg

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WB (Western Blot) (P15RS monoclonal antibody. Western Blot analysis of P15RS expression in NIH/3T3.)

Application Data (Detection limit for recombinant GST tagged P15RS is ~0.03ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to P15RS on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3ug/ml].)

WB (Western Blot) (P15RS monoclonal antibody, Western Blot analysis of P15RS expression in A-431.)

WB (Western Blot) (P15RS monoclonal antibody. Western Blot analysis of P15RS expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (36.56kD).)

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P15RS, Monoclonal Antibody (Cat# AAA25777)

Full Name

P15RS (RPRD1A, Regulation of Nuclear Pre-mRNA Domain-containing Protein 1A, Cyclin-dependent Kinase Inhibitor 2B-related Protein, p15INK4B-related Protein, FLJ10656, HsT3101, MGC19513) (PE)

Gene Names

RPRD1A; P15RS; HsT3101

Reactivity

Human, Mouse, Rat

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Staining of Rat peripheral blood lymphocytes with Mouse anti Rat CD49d:RPE (MCA2872PE))

Application Data (Staining of Rat peripheral blood lymphocytes with Mouse anti Rat CD49d : Alexa Fluor® 488 (MCA2872A488))

Application Data (Staining of Rat peripheral blood lymphocytes with Mouse anti Rat CD49d : Alexa Fluor® 647 (MCA2872A647))

Application Data (Staining of Rat peripheral blood lymphocytes with Mouse anti Rat CD49d (MCA2872)

Application Data (Staining of Rat peripheral blood lymphocytes with Mouse anti Rat CD49d:FITC (MCA2872F)

Application Data (Staining of Rat peripheral blood lymphocytes with Mouse anti Rat CD49d (MCA2872GA))

Application Data (Staining of Rat peripheral blood lymphocytes with Mouse anti Rat CD49d (MCA2872T))

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CD49d, Monoclonal Antibody (Cat# AAA26812)

Full Name

CD49d (Antigen CD49d, CD49d Antigen, CDw49d, Alpha 4 Subunit of VLA-4 Receptor, Integrin alpha IV, Integrin alpha 4, IA4, ITGA4, LPAM23, MGC90518, Very Late Activation Protein 4 Receptor Alpha 4 Subunit, VLA4, VLA-4) (MaxLight 550)

Reactivity

Rat

Applications

FC/FACS, IP

Purity

Purified by Protein G Affinity Chromatography

Pricing

$1,010/0.1 mL $1,950/2x0.1 mL $2,835/3x0.1 mL $3,675/4x0.1 mL $4,260/5x0.1 mL

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Application Data (Staining of mouse thymus with RAT ANTI MOUSE CD150: RPE)

Application Data (Staining of mouse thymus with RAT ANTI MOUSE CD150: AZIDE FREE)

Application Data (Staining of mouse thymus with RAT ANTI MOUSE CD150)

Application Data (Staining of mouse thymus cells with RAT ANTI MOUSE CD150:ALEXA 647)

Application Data (Staining of mouse thymus cells with RAT ANTI MOUSE CD150: Alexa Fluor® 488)

Application Data (Staining of mouse thymus cells with RAT ANTI MOUSE CD150)

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CD150, Monoclonal Antibody (Cat# AAA26818)

Full Name

CD150 (CD150 Antigen, Cdw150, 4933415F16, Estm51, Ipo 3, Ipo-3, OTTHUMP00000060252, Signaling Lymphocytic Activation Molecule, SLAM, Signaling Lymphocytic Activation Molecule Family Member 1, SLAMF1 Protein) (MaxLight 650)

Reactivity

Mouse

Applications

FC/FACS

Purity

Purified by Protein G Affinity Chromatography

Pricing

$1,010/0.1 mL $1,950/2x0.1 mL $2,835/3x0.1 mL $3,675/4x0.1 mL $4,260/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged HDAC1 is approximately 1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HDAC1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 0.7 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HDAC1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 0.7 ug/ml])

WB (Western Blot) (HDAC1 monoclonal antibody (M11), clone 5A11 Western Blot analysis of HDAC1 expression in Hela S3 NE (Cat # L013V3).)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HDAC1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HDAC1 on HeLa cell. [antibody concentration 10 ug/ml])

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HDAC1, Monoclonal Antibody (Cat# AAA26320)

Full Name

HDAC1 (Histone Deacetylase 1, DKFZp686H12203, GON-10, HD1, RPD3, RPD3L1) (FITC)

Gene Names

HDAC1; HD1; RPD3; KDAC1; GON-10; RPD3L1

Applications

IF, IHC, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

SEC-HPLC (The purity of this product is >95% as determined by SEC-MALS.)

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SEZ6, Monoclonal Recombinant Antibody (Cat# AAA18899)

Full Name

Anti-Human SEZ6 Antibody (SAA1693)

Reactivity

Human

Applications

EIA

Purity

>95% by SDS-PAGE
Protein A or G purified from cell culture supernatant.

Pricing

$505/0.1 mg $1,665/1 mg $3,220/2x1 mg $4,685/3x1 mg $6,075/4x1 mg $6,940/5x1 mg

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WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between AKT1 and HSPB1. Mahlavu cells were stained with AKT1 rabbit purified polyclonal 1:1200 and HSPB1 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Application Data (Detection limit for recombinant GST tagged HSPB1 is ~0.1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of HSPB1 transfected lysate using HSPB1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HSPB1 rabbit polyclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HSPB1 on HeLa cell. [antibody concentration 10ug/ml])

WB (Western Blot) (Western Blot analysis of HSPB1 expression in transfected 293T cell line by HSPB1 monoclonal antibody. Lane 1: HSPB1 transfected lysate (22.8kD). Lane 2: Non-transfected lysate.)

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HSPB1, Monoclonal Antibody (Cat# AAA24539)

Full Name

HSPB1 (Heat Shock Protein beta-1, HspB1, Heat Shock 27kD Protein, HSP 27, Stress-responsive Protein 27, SRP27, Estrogen-regulated 24kD Protein, 28kD Heat Shock Protein, HSPB1, HSP27) APC

Gene Names

HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102

Reactivity

Human

Applications

EIA, IF, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (CDK6 monoclonal antibody, Western Blot analysis of CDK6 expression in Jurkat.)

WB (Western Blot) (CDK6 monoclonal antibody. Western Blot analysis of CDK6 expression in PC-12.)

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between CDK2 and CDK6 HeLa cells were stained with CDK2 rabbit purified polyclonal 1:1200 and CDK6 mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CDK6 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CDK6 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of CDK6 expression in transfected 293T cell line by CDK6 monoclonal antibody. Lane 1: CDK6 transfected lysate (36.9kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (36.41kD).)

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CDK6, Monoclonal Antibody (Cat# AAA25053)

Full Name

CDK6 (Cell Division Protein Kinase 6, Serine/Threonine-protein Kinase PLSTIRE, Crk2, MGC59692) (FITC)

Gene Names

CDK6; MCPH12; PLSTIRE

Reactivity

Human, Rat

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Histone H2B antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining Histone H2B in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H2B antibody. Counter stained with hematoxylin.)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H2B antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Histone H2B antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Histone H2B antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Histone H2B antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H2B antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Histone H2B on different lysates using anti-Histone H2B antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3 Lane 3: PC12)

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Histone H2B, Monoclonal Antibody (Cat# AAA30192)

Full Name

Histone H2B Antibody

Gene Names

HIST1H2BK; H2BK; H2B/S; H2BFT; H2BFAiii

Reactivity

Human, Mouse, Rat

Applications

WB, ICC, IF, IHC, IP, FC/FACS

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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WB (Western Blot) (F3 monoclonal antibody, Western Blot analysis of F3 expression in A-431.)

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between F7 and F3. HeLa cells were stained with F7 rabbit purified polyclonal 1:1200 and F3 mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex)

Application Data (Detection limit for recombinant GST tagged F3 is ~0.03ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of F3 transfected lysate using F3 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with F3 rabbit polyclonal antibody.)

WB (Western Blot) (Western Blot analysis of F3 expression in transfected 293T cell line by F3 monoclonal antibody. Lane 1: F3 transfected lysate (33.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)

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CD142, Monoclonal Antibody (Cat# AAA25343)

Full Name

CD142 (CD142 Antigen, Coagulation Factor III, F3, Tissue Factor, TF, TFA, Thromboplastin) (HRP)

Gene Names

F3; TF; TFA; CD142

Reactivity

Human

Applications

EIA, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Detection of human VISTA by western blot. Samples: Whole cell lysate (10 ug) from HeLa, Jurkat, HEK293T, KG-1, and RPMI-8226 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-VISTA recombinant monoclonal antibody [BL-226-5C12] (AAA23805 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

IP (Immunoprecipitation) (Detection of human VISTA by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-VISTA recombinant monoclonal antibody [BL-226-5C12] (AAA23805 lot 3) used for IP at 20 ul/mg lysate. VISTA was also immunoprecipitated by a previous lot of this antibody (AAA23805 lot 2). For blotting immunoprecipitated VISTA, AAA23805 was used at 1:1000. Chemiluminescence with an exposure time of 10 seconds.)

IHC (Immunohistochemistry) (Detection of human VISTA (red) by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Rabbit anti-VISTA recombinant monoclonal antibody (AAA23805) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI (blue).)

IHC (Immunohistochemistry) (Detection of human VISTA by immunohistochemistry. Sample: FFPE section of colon carcinoma. Antibody: Rabbit anti-VISTA recombinant monoclonal antibody (AAA23805-3). Secondary: HRP-conjugated goat anti-rabbit IgG .)

ICC (Immunocytochemistry) (Detection of human VISTA by immunocytochemistry. Sample: FFPE section of NCI-H226 cells. Antibody: Rabbit anti-VISTA recombinant monoclonal antibody (AAA23805-3). Secondary: HRP-conjugated goat anti-rabbit IgG .)

FCM (Flow Cytometry) (Detection of human VISTA in human peripheral blood mononuclear cells by flow cytometry. Antibody: Rabbit anti-VISTA recombinant monoclonal (AAA23805) (right) or isotype control (left). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)

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VISTA, Monoclonal Recombinant Antibody (Cat# AAA23805)

Full Name

Rabbit anti-VISTA Recombinant Monoclonal Antibody [BLR035F]

Gene Names

C10orf54; B7H5; GI24; B7-H5; SISP1; VISTA; PP2135; DD1alpha

Reactivity

Human

Applications

WB, IP, IHC, ICC, IHC-IF, FC/FACS

Purity

Purified

Pricing

$295/0.01 mL $680/0.1 mL $980/5x0.01 mL $1,300/2x0.1 mL $1,890/3x0.1 mL $2,440/4x0.1 mL $2,755/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of Jurkat cells with RUNX1+RUNX2+RUNX3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining RUNX1+RUNX2+RUNX3 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining RUNX1+RUNX2+RUNX3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining RUNX1+RUNX2+RUNX3 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-RUNX1+RUNX2+RUNX3 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-RUNX1+RUNX2+RUNX3 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of RUNX1+RUNX2+RUNX3 on Jurkat cells lysates using anti-RUNX1+RUNX2+RUNX3 antibody at 1/1, 000 dilution.)

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RUNX1+RUNX2+RUNX3, Monoclonal Antibody (Cat# AAA30202)

Full Name

RUNX1+RUNX2+RUNX3 Antibody

Gene Names

RUNX1; AML1; CBFA2; EVI-1; AMLCR1; PEBP2aB; AML1-EVI-1

Reactivity

Human, Mouse, Rat

Applications

WB, ICC, IF, IHC, IP, FC/FACS

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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IF (Immunofluorescence) (Figure 10. IF analysis of EIF4A1 using anti-EIF4A1 antibody (AAA19711).EIF4A1 was detected in an immunocytochemical section of HEP3B cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL mouse anti-EIF4A1 Antibody (AAA19711) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of U87 cells using anti-EIF4A1 antibody (AAA19711).Overlay histogram showing U87 cells stained with AAA19711 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-EIF4A1 Antibody (AAA19711, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of RH35 cells using anti-EIF4A1 antibody (AAA19711).Overlay histogram showing RH35 cells stained with AAA19711 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-EIF4A1 Antibody (AAA19711, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of ANA-1 cells using anti-EIF4A1 antibody (AAA19711).Overlay histogram showing ANA-1 cells stained with AAA19711 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-EIF4A1 Antibody (AAA19711, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of EIF4A1 using anti-EIF4A1 antibody (AAA19711).EIF4A1 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-EIF4A1 Antibody (AAA19711) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of EIF4A1 using anti-EIF4A1 antibody (AAA19711).EIF4A1 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-EIF4A1 Antibody (AAA19711) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of EIF4A1 using anti-EIF4A1 antibody (AAA19711).EIF4A1 was detected in a paraffin-embedded section of human tonsli tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-EIF4A1 Antibody (AAA19711) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of EIF4A1 using anti-EIF4A1 antibody (AAA19711).EIF4A1 was detected in a paraffin-embedded section of human colonic adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-EIF4A1 Antibody (AAA19711) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of EIF4A1 using anti-EIF4A1 antibody (AAA19711).EIF4A1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-EIF4A1 Antibody (AAA19711) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of EIF4A1 using anti-EIF4A1 antibody (AAA19711).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human Hek293 whole cell lysates,Lane 3: human U87 whole cell lysates,Lane 4: human K562 whole cell lysates,Lane 5: rat spleen tissue lysates,Lane 6: rat PC-12 whole cell lysates,Lane 7: mouse RAW264.7 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-EIF4A1 antigen affinity purified monoclonal antibody (#AAA19711) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for EIF4A1 at approximately 47 kDa. The expected band size for EIF4A1 is at 47kDa.)

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EIF4A1, Monoclonal Antibody (Cat# AAA19711)

Full Name

Anti-EIF4A1 Antibody Picoband (monoclonal, 3F11)

Gene Names

EIF4A1; DDX2A; EIF4A; EIF-4A; eIF4A-I; eIF-4A-I

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ICC, IF, FC/FACS

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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IHC (Immunohistochemistry) (Figure 14. IHC analysis of splicing factor 1 using anti-splicing factor 1 antibody (AAA19677).splicing factor 1 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-splicing factor 1 Antibody (AAA19677) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 13. IHC analysis of splicing factor 1 using anti-splicing factor 1 antibody (AAA19677).splicing factor 1 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-splicing factor 1 Antibody (AAA19677) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 12. IHC analysis of splicing factor 1 using anti-splicing factor 1 antibody (AAA19677).splicing factor 1 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-splicing factor 1 Antibody (AAA19677) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 11. IHC analysis of splicing factor 1 using anti-splicing factor 1 antibody (AAA19677).splicing factor 1 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-splicing factor 1 Antibody (AAA19677) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of C6 cells using anti-splicing factor 1 antibody (AAA19677).Overlay histogram showing C6 cells stained with AAA19677 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-splicing factor 1 Antibody (AAA19677, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of Neuro-2a cells using anti-splicing factor 1 antibody (AAA19677).Overlay histogram showing Neuro-2a cells stained with AAA19677 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-splicing factor 1 Antibody (AAA19677, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of A431 cells using anti-splicing factor 1 antibody (AAA19677).Overlay histogram showing A431 cells stained with AAA19677 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-splicing factor 1 Antibody (AAA19677, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 7. IF analysis of splicing factor 1 using anti-splicing factor 1 antibody (AAA19677).splicing factor 1 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL mouse anti-splicing factor 1 Antibody (AAA19677) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of splicing factor 1 using anti-splicing factor 1 antibody (AAA19677).splicing factor 1 was detected in a paraffin-embedded section of human squamous metaplasia of the renal pelvis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-splicing factor 1 Antibody (AAA19677) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of splicing factor 1 using anti-splicing factor 1 antibody (AAA19677).splicing factor 1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-splicing factor 1 Antibody (AAA19677) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of splicing factor 1 using anti-splicing factor 1 antibody (AAA19677).splicing factor 1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-splicing factor 1 Antibody (AAA19677) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of splicing factor 1 using anti-splicing factor 1 antibody (AAA19677).splicing factor 1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-splicing factor 1 Antibody (AAA19677) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of splicing factor 1 using anti-splicing factor 1 antibody (AAA19677).splicing factor 1 was detected in a paraffin-embedded section of human bladder cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-splicing factor 1 Antibody (AAA19677) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of splicing factor 1 using anti-splicing factor 1 antibody (AAA19677).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human SKOV-3 whole cell lysates,Lane 2: human K562 whole cell lysates,Lane 3: human THP-1 whole cell lysates,Lane 4: human A549 whole cell lysates,Lane 5: rat PC-12 whole cell lysates,Lane 6: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-splicing factor 1 antigen affinity purified monoclonal antibody (#AAA19677) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for splicing factor 1 at approximately 68 kDa. The expected band size for splicing factor 1 is at 68 kDa.)

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splicing factor 1, Monoclonal Antibody (Cat# AAA19677)

Full Name

Anti-splicing factor 1 Antibody Picoband (monoclonal, 2F5D10)

Gene Names

SF1; BBP; MBBP; ZFM1; ZNF162; D11S636; ZCCHC25

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ICC, IF, FC/FACS

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti MouseE CD49d: Low Endotoxin)

Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD49d: Alexa Fluor 647)

Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD49d: FITC)

Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD49d)

Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD49d)

Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD49d: Azide Free)

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CD49d, Monoclonal Antibody (Cat# AAA11924)

Full Name

RAT ANTI MOUSE CD49d

Gene Names

Itga4; CD49D; Itga4B

Applications

Immunohistochemistry, Flow Cytometry, Immunoprecipitation

Pricing

$440/0.1 mg $840/2x0.1 mg $1,205/3x0.1 mg $1,555/4x0.1 mg $1,680/5x0.1 mg

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Application Data (Analysis of Protein Array containing >19, 000 full-length human proteins using TACSTD2 Mouse Monoclonal Antibody (TACSTD2/2153) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

SDS-PAGE (SDS-PAGE Analysis Purified TACSTD2 / TROP2Mouse Monoclonal Antibody (TACSTD2/2153).Confirmation of Integrity and Purity of Antibody.)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embeddedhuman PancreaticCarcinoma stained with TACSTD2 / TROP2Mouse Monoclonal Antibody (TACSTD2/2153).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embeddedhuman PancreaticCarcinoma stained with TACSTD2 / TROP2Mouse Monoclonal Antibody (TACSTD2/2153).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embeddedhuman Basal Cell Carcinoma stained with TACSTD2 / TROP2Mouse Monoclonal Antibody (TACSTD2/2153).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embeddedhuman Colon Carcinoma stained with TACSTD2 / TROP2Mouse Monoclonal Antibody (TACSTD2/2153).)

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TACSTD2/TROP2, Monoclonal Antibody (Cat# AAA23900)

Full Name

TACSTD2/TROP2 (Epithelial Marker)

Gene Names

TACSTD2; EGP1; GP50; M1S1; EGP-1; TROP2; GA7331; GA733-1

Reactivity

Human. Others not known.

Applications

Immunohistochemistry

Pricing

$295/0.02 mg (With BSA & Azide at 0.2mg/mL) $475/0.1 mg (With BSA & Azide at 0.2mg/mL) $475/0.1 mg (Without BSA & Azide at 1mg/mL) $905/2x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,310/3x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,690/4x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,900/5x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,900/5x0.1 mg (Without BSA & Azide at 1mg/mL)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

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CRYM, Monoclonal Antibody (Cat# AAA24177)

Full Name

CRYM (Thiomorpholine-carboxylate Dehydrogenase, Mu-crystallin Homolog, NADP-regulated Thyroid-hormone-binding Protein, Ketimine Reductase, THBP) (AP)

Gene Names

CRYM; THBP; DFNA40

Reactivity

Human

Applications

EIA, IHC, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western blot analysis of SGK over-expressed 293 cell line, cotransfected with SGK Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with SGK monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged SGK is ~1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SGK on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SGK on formalin-fixed paraffin-embedded human stomach tissue. [antibody concentration 5ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SGK on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of SGK expression in transfected 293T cell line by SGK monoclonal antibody. Lane 1: SGK transfected lysate (48.9kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (73.52kD).)

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SGK, Monoclonal Antibody (Cat# AAA25539)

Full Name

SGK (SGK1, Serine/Threonine-protein Kinase SGK1, Serum/Glucocorticoid-regulated Kinase 1) (HRP)

Gene Names

SGK1; SGK

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (CAMKK1 monoclonal antibody, Western Blot analysis of CAMKK1 expression in Jurkat.)

Application Data (Detection limit for recombinant GST tagged CAMKK1 is ~1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CAMKK1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1ug/ml].)

WB (Western Blot) (Western Blot analysis of CAMKK1 expression in transfected 293T cell line by CAMKK1 monoclonal antibody. Lane 1: CAMKK1 transfected lysate (57.31kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western blot analysis of CAMKK1 over-expressed 293 cell line, cotransfected with CAMKK1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CAMKK1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

WB (Western Blot) (Western Blot detection against Immunogen (83.31kD).)

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CAMKK1, Monoclonal Antibody (Cat# AAA24744)

Full Name

CAMKK1 (Calcium/Calmodulin-dependent Protein Kinase Kinase 1, CaM-kinase Kinase 1, CaM-KK 1, CaMKK 1, CaMKK 1, Calcium/Calmodulin-dependent Protein Kinase Kinase alpha, CaM-kinase Kinase alpha, CaM-KK alpha, CaMKK alpha, CaM-kinase IV Kinase, CAMKKA, DKFZ

Gene Names

CAMKK1; CAMKKA

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (EFHD1 monoclonal antibody (M05), Western Blot analysis of EFHD1 expression in HeLa.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to EFHD1 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3ug/ml].)

WB (Western Blot) (EFHD1 monoclonal antibody (M05). Western Blot analysis of EFHD1 expression in NIH/3T3.)

WB (Western Blot) (EFHD1 monoclonal antibody. Western Blot analysis of EFHD1 expression in Raw 264.7.)

WB (Western Blot) (EFHD1 monoclonal antibody. Western Blot analysis of EFHD1 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (33.81kD).)

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EFHD1, Monoclonal Antibody (Cat# AAA24787)

Full Name

EFHD1 (EF-hand Domain-containing Protein D1, EF-hand Domain-containing Protein 1, Swiprosin-2, SWS2, PP3051) (Biotin)

Gene Names

EFHD1; SWS2; MST133; PP3051; MSTP133; FLJ13612; DKFZp781H0842

Reactivity

Human, Mouse, Rat

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Staining of mouse thymus with RAT ANTI MOUSE CD150: RPE)

Application Data (Staining of mouse thymus with RAT ANTI MOUSE CD150: AZIDE FREE)

Application Data (Staining of mouse thymus with RAT ANTI MOUSE CD150)

Application Data (Staining of mouse thymus cells with RAT ANTI MOUSE CD150:ALEXA 647)

Application Data (Staining of mouse thymus cells with RAT ANTI MOUSE CD150: Alexa Fluor® 488)

Application Data (Staining of mouse thymus cells with RAT ANTI MOUSE CD150)

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CD150, Monoclonal Antibody (Cat# AAA26832)

Full Name

CD150 (CD150 Antigen, Cdw150, 4933415F16, Estm51, Ipo 3, Ipo-3, OTTHUMP00000060252, Signaling Lymphocytic Activation Molecule, SLAM, Signaling Lymphocytic Activation Molecule Family Member 1, SLAMF1 Protein) (MaxLight 750)

Reactivity

Mouse

Applications

FC/FACS

Purity

Purified by Protein G Affinity Chromatography

Pricing

$950/0.1 mL $1,830/2x0.1 mL $2,660/3x0.1 mL $3,440/4x0.1 mL $3,985/5x0.1 mL

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ICC (Immunocytochemistry) (ICC staining IRF3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining IRF3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-IRF3 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-IRF3 antibody. Counter stained with hematoxylin.)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-IRF3 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-IRF3 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-IRF3 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-IRF3 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-IRF3 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of IRF3 on different lysates using anti-IRF3 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat Lane 3: THP-1)

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IRF3, Monoclonal Antibody (Cat# AAA30188)

Full Name

IRF3 Antibody

Reactivity

Human, Mouse, Rat

Applications

WB, ICC, IF, IHC, FC/FACS

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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WB (Western Blot) (Western blot analysis of GSR over-expressed 293 cell line, cotransfected with GSR Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with GSR monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged GSR is ~0.03ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GSR on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 6ug/ml].)

WB (Western Blot) (Western Blot analysis of GSR expression in transfected 293T cell line by GSR monoclonal antibody. Lane 1: GSR transfected lysate (56.3kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (GSR monoclonal antibody Western Blot analysis of GSR expression in IMR-32.)

WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)

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GSR, Monoclonal Antibody (Cat# AAA24821)

Full Name

GSR (Glutathione Reductase, Mitochondrial, GR, GRase, GSR, GLUR, GRD1, MGC78522) (Biotin)

Gene Names

GSR; HEL-75; HEL-S-122m

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of HL-60 cells using anti-PARP antibody (AAA19346).Overlay histogram showing HL-60 cells stained with AAA19346 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- PARP Antibody (AAA19346, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 6. IF analysis of PARP using anti- PARP antibody (AAA19346).PARP was detected in immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti- PARP Antibody (AAA19346) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of PARP using anti-PARP antibody (AAA19346).PARP was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-PARP Antibody (AAA19346) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of PARP using anti-PARP antibody (AAA19346).PARP was detected in paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-PARP Antibody (AAA19346) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of PARP using anti-PARP antibody (AAA19346).PARP was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-PARP Antibody (AAA19346) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of PARP using anti-PARP antibody (AAA19346).PARP was detected in paraffin-embedded section of human gallbladder adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-PARP Antibody (AAA19346) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of PARP using anti-PARP antibody (AAA19346).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human MCF-7 whole cell lysatesLane 2: human HELA whole cell lysatesLane 3: human HEK293 whole cell lysatesLane 4: rat testis tissue lysatesLane 5: mouse testis tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- PARP antigen affinity purified monoclonal antibody (Catalog # AAA19346) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for PARP at approximately 113KD. The expected band size for PARP is at 113KD.)

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PARP, Monoclonal Antibody (Cat# AAA19346)

Full Name

Anti-PARP Antibody (monoclonal, 10G9)

Gene Names

PARP1; PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, ICC, IF, FC/FACS/FCM

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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WB (Western Blot) (MAP3K7IP1 monoclonal antibody Western Blot analysis of MAP3K7IP1 expression in PC-12.)

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between HSPA1L and MAP3K7IP1 HeLa cells were stained with HSPA1L rabbit purified polyclonal 1:1200 and MAP3K7IP1 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Application Data (Detection limit for recombinant GST tagged MAP3K7IP1 is ~0.3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MAP3K7IP1 on HeLa cell. [antibody concentration 10ug/ml])

WB (Western Blot) (MAP3K7IP1 monoclonal antibody Western Blot analysis of MAP3K7IP1 expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (36.89kD).)

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TAB1, Monoclonal Antibody (Cat# AAA25270)

Full Name

TAB1 (TGF-beta-activated Kinase 1 and MAP3K7-binding Protein 1, Mitogen-activated Protein Kinase Kinase Kinase 7-interacting Protein 1, TGF-beta-activated Kinase 1-binding Protein 1, TAK1-binding Protein 1, MAP3K7IP1) (FITC)

Gene Names

TAB1; 3'-Tab1; MAP3K7IP1

Reactivity

Human, Rat

Applications

EIA, IF, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (DCPS monoclonal antibody. Western Blot analysis of DCPS expression in PC-12.)

Application Data (Detection limit for recombinant GST tagged DCPS is ~3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to DCPS on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to DCPS on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1.5ug/ml])

WB (Western Blot) (DCPS monoclonal antibody Western Blot analysis of DCPS expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (63.18kD).)

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DCPS, Monoclonal Antibody (Cat# AAA14781)

Full Name

DCPS (DCS1, HINT5, m7GpppX Diphosphatase, DCS-1, Hint-related 7meGMP-directed Hydrolase, Histidine Triad Protein Member 5, Scavenger mRNA-decapping Enzyme DcpS, HSPC015)

Gene Names

DCPS; HINT-5

Reactivity

Human, Rat

Applications

EL/EIA, WB, IHC, IF

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

$820/0.1 mg $1,565/2x0.1 mg $2,270/3x0.1 mg $2,940/4x0.1 mg $3,380/5x0.1 mg

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WB (Western Blot) (PPIE monoclonal antibody Western Blot analysis of PPIE expression in PC-12.)

WB (Western Blot) (PPIE monoclonal antibody Western Blot analysis of PPIE expression in Hela NE.)

Application Data (Detection limit for recombinant GST tagged PPIE is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PPIE on HeLa cell. [antibody concentration 10ug/ml])

WB (Western Blot) (Western Blot analysis of PPIE expression in transfected 293T cell line by PPIE monoclonal antibody. Lane 1: PPIE transfected lysate (33.4kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (PPIE monoclonal antibody Western Blot analysis of PPIE expression in NIH/3T3.)

WB (Western Blot) (PPIE monoclonal antibody Western Blot analysis of PPIE expression in Raw 264.7.)

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Cyclophilin E, Monoclonal Antibody (Cat# AAA24773)

Full Name

Cyclophilin E (Cyclophilin 33, CYP33, CYP-33, MGC3736, MGC111222, Peptidyl-Prolyl Cis-Trans Isomerase E, PPIase E, PPIE, Rotamase E, Rotamase-E) (Biotin)

Gene Names

PPIE; CYP33; CYP-33

Reactivity

Human, Mouse, Rat

Applications

EIA, IF, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (SMAD3 monoclonal antibody (M01), clone 2C12. Western Blot analysis of SMAD3 expression in Hs 181.Tes.)

WB (Western Blot) (SMAD3 monoclonal antibody (M01), clone 2C12. Western Blot analysis of SMAD3 expression in HeLa.)

WB (Western Blot) (Western Blot analysis of SMAD3 expression in transfected 293T cell line by SMAD3 monoclonal antibody (M01), clone 2C12.Lane 1: SMAD3 transfected lysate (48 KDa).Lane 2: Non-transfected lysate.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SMAD3 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 6 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SMAD3 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 6 ug/ml])

WB (Western Blot) (SMAD3 monoclonal antibody (M01), clone 2C12. Western Blot analysis of SMAD3 expression in human colon.)

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SMAD3, Monoclonal Antibody (Cat# AAA26562)

Full Name

SMAD3 (SMAD Family Member 3, DKFZp586N0721, DKFZp686J10186, HSPC193, HsT17436, JV15-2, MADH3, MGC60396) (PE)

Gene Names

SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436

Applications

IHC, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged GBA is ~1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GBA on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GBA on formalin-fixed paraffin-embedded human breast cancer. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of GBA expression in transfected 293T cell line by GBA monoclonal antibody. Lane 1: GBA transfected lysate (60kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (GBA monoclonal antibody, Western Blot analysis of GBA expression in MCF-7.)

WB (Western Blot) (Western Blot detection against Immunogen (35.64kD).)

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GBA, Monoclonal Antibody (Cat# AAA24518)

Full Name

GBA (Glucosylceramidase, Acid beta-glucosidase, Alglucerase, Beta-glucocerebrosidase, D-glucosyl-N-acylsphingosine Glucohydrolase, Imiglucerase, GC, GLUC) APC

Gene Names

GBA; GCB; GBA1; GLUC

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (PKNOX2 monoclonal antibody. Western Blot analysis of PKNOX2 expression in NIH/3T3.)

WB (Western Blot) (Western blot analysis of PKNOX2 over-expressed 293 cell line, cotransfected with PKNOX2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PKNOX2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PKNOX2 on HeLa cell. [antibody concentration 10ug/ml].)

WB (Western Blot) (Western Blot analysis of PKNOX2 expression in transfected 293T cell line by PKNOX2 monoclonal antibody. Lane 1: PKNOX2 transfected lysate (51.9kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (PKNOX2 monoclonal antibody, Western Blot analysis of PKNOX2 expression in Hela NE.)

WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)

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PKNOX2, Monoclonal Antibody (Cat# AAA25798)

Full Name

PKNOX2 (PREP2, Homeobox Protein PKNOX2, Homeobox Protein PREP-2, PBX/Knotted Homeobox 2, FLJ13074) (PE)

Gene Names

PKNOX2; PREP2

Reactivity

Human, Mouse

Applications

EIA, IF, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (DNAJC10 monoclonal antibody. Western Blot analysis of DNAJC10 expression in HeLa.)

WB (Western Blot) (DNAJC10 monoclonal antibody. Western Blot analysis of DNAJC10 expression in Raw 264.7.)

Application Data (Detection limit for recombinant GST tagged DNAJC10 is ~10ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to DNAJC10 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to DNAJC10 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3ug/ml].)

WB (Western Blot) (DNAJC10 monoclonal antibody. Western Blot analysis of DNAJC10 expression in NIH/3T3.)

WB (Western Blot) (Western Blot detection against Immunogen (37.77kD).)

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DNAJC10, Monoclonal Antibody (Cat# AAA24784)

Full Name

DNAJC10 (DnaJ Homolog Subfamily C Member 10, ER-resident Protein ERdj5, Macrothioredoxin, MTHr, ERDJ5, UNQ495/PRO1012, DKFZp434J1813, MGC104194) (Biotin)

Gene Names

DNAJC10; JPDI; MTHr; ERdj5; PDIA19

Reactivity

Human, Mouse, Rat

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged MTAP is ~0.3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MTAP on HeLa cell. [antibody concentration 10ug/ml])

WB (Western Blot) (MTAP monoclonal antibody. Western Blot analysis of MTAP expression in NIH/3T3.)

WB (Western Blot) (MTAP monoclonal antibody. Western Blot analysis of MTAP expression in Raw 264.7.)

WB (Western Blot) (MTAP monoclonal antibody. Western Blot analysis of MTAP expression in PC-12.)

WB (Western Blot) (MTAP monoclonal antibody Western Blot analysis of MTAP expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (42.68kD).)

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MTAP, Monoclonal Antibody (Cat# AAA24280)

Full Name

MTAP (methylthioadenosine phosphorylase, 5'-methylthioadenosine phosphorylase, c86fus, MSAP, MTAPase, MTA Phosphorylase, S-methyl-5'-thioadenosine Phosphorylase) (AP)

Gene Names

MTAP; BDMF; MSAP; DMSFH; LGMBF; DMSMFH; c86fus; HEL-249

Reactivity

Human, Mouse, Rat

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of ACY1 expression in transfected 293T cell line by ACY1 monoclonal antibody. Lane 1: ACY1 transfected lysate (45.9kD). Lane 2: Non-transfected lysate.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ACY1 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ACY1 on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B tissue. [antibody concentration 3ug/ml])

IP (Immunoprecipitation) (Immunoprecipitation of ACY1 transfected lysate using ACY1 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with ACY1 rabbit polyclonal antibody.)

WB (Western Blot) (ACY1 monoclonal antibody Western Blot analysis of ACY1 expression in K-562.)

WB (Western Blot) (Western Blot detection against Immunogen (70.62kD).)

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ACY1, Monoclonal Antibody (Cat# AAA25306)

Full Name

ACY1 (Aminoacylase 1, Aminoacylase-1, ACY-1, N-acyl-L-amino-acid Amidohydrolase) (HRP)

Gene Names

ACY1; ACY-1; ACY1D; HEL-S-5

Reactivity

Human

Applications

EIA, IHC, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western Blot detection against Immunogen (37kD).)

Application Data (Detection limit for recombinant GST tagged GMNN is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GMNN on HeLa cell . [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GMNN on formalin-fixed paraffin-embedded human placenta. [antibody concentration 1ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GMNN on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of GMNN expression in transfected 293T cell line by GMNN monoclonal antibody.Lane 1: GMNN transfected lysate (23.6kD).Lane 2: Non-transfected lysate.)

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Geminin, Monoclonal Antibody (Cat# AAA24039)

Full Name

Geminin (GMNN, GEM, Geminin DNA Replication Inhibitor, DNA Replication Inhibitor, RP3-369A17.3)

Gene Names

GMNN; Gem

Reactivity

Human

Applications

EL/EIA, WB, IHC, IF

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

$820/0.1 mg $1,565/2x0.1 mg $2,270/3x0.1 mg $2,940/4x0.1 mg $3,380/5x0.1 mg

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WB (Western Blot) (Detection of mouse BRD4 by western blot. Samples: Whole cell lysate (50 ug) from BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-151-6F11] (AAA23787 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds.)

WB (Western Blot) (Detection of human BRD4 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, Jurkat, MDA-MB-231, Hep-G2, A-549, SW620, and SK-MEL-28 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-151-6F11] (AAA23787 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-GAPDH .)

IP (Immunoprecipitation) (Detection of human BRD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-151-6F11] (AAA23787 lot 3) used for IP at 20 ul/mg lysate. BRD4 was also immunoprecipitated by a previous lot of this antibody (lot AAA23787-2). For blotting immunoprecipitated BRD4, AAA23787 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)

IHC (Immunohistochemistry) (Detection of human BRD4 by immunohistochemistry. Sample: FFPE section of ovarian carcinoma. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-151-6F11] (AAA23787 lot 1) used at 1:50. Secondary: DyLight 594-conjugated goat anti-rabbit IgG antibody .)

IHC (Immunohistochemistry) (Detection of human BRD4 in FFPE ovarian carcinoma by IHC. Antibody: Rabbit anti-BRD4 recombinant monoclonal [BL-151-6F11] (AAA23787 lot 3). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry) (Detection of human BRD4 by immunocytochemistry. Sample: formaldehyde-fixed Hep-G2 cells. Antibody: Rabbit anti-BRD4 recombinant monoclonal antibody [BL-151-6F11] (AAA23787 lot 1) used at 1:50. Secondary: DyLight 594-conjugated goat anti-rabbit IgG antibody .)

FCM (Flow Cytometry) (Detection of human BRD4 (shaded) in KG-1 cells by flow cytometry. Antibody: Rabbit anti-BRD4 recombinant monoclonal [BL-151-6F11] (AAA23787) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

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BRD4, Monoclonal Recombinant Antibody (Cat# AAA23787)

Full Name

Rabbit anti-BRD4 Recombinant Monoclonal Antibody [BL-151-6F11]

Gene Names

BRD4; CAP; MCAP; HUNK1; HUNKI

Reactivity

Human, Mouse

Applications

WB, IP, IHC, IHC-IF, ICC-IF, FC/FACS

Purity

Purified

Pricing

$295/0.02 mL $480/0.1 mL $915/2x0.1 mL $980/5x0.02 mL $1,325/3x0.1 mL $1,705/4x0.1 mL $1,855/5x0.1 mL

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WB (Western Blot) (Western blot analysis of PIP5K3 over-expressed 293 cell line, cotransfected with PIP5K3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PIP5K3 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged PIP5K3 is ~0.3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PIP5K3 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PIP5K3 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of PIP5K3 expression in transfected 293T cell line by PIP5K3 monoclonal antibody.Lane 1: PIP5K3 transfected lysate (50.2kD).Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (38.21kD).)

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PIP5K3, Monoclonal Antibody (Cat# AAA24058)

Full Name

PIP5K3 (PIKFYVE, 1-phosphatidylinositol 3-phosphate 5-kinase, Phosphatidylinositol 3-phosphate 5-kinase, FYVE Finger-containing Phosphoinositide Kinase, PIKfyve, Phosphatidylinositol 3-phosphate 5-kinase Type III, PIPkin-III, Type III PIP Kinase, KIAA0981

Gene Names

PIKFYVE; CFD; FAB1; PIP5K; PIP5K3; ZFYVE29

Reactivity

Human

Applications

EL/EIA, WB, IHC, IF

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

$820/0.1 mg $1,565/2x0.1 mg $2,270/3x0.1 mg $2,940/4x0.1 mg $3,380/5x0.1 mg

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WB (Western Blot) (Western blot analysis of PDK2 over-expressed 293 cell line, cotransfected with PDK2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PDK2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged PDK2 is ~0.3ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PDK2 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 0.8ug/ml])

WB (Western Blot) (Western Blot analysis of PDK2 expression in transfected 293T cell line by PDK2 monoclonal antibody. Lane 1: PDK2 transfected lysate (46.2kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (PDK2 monoclonal antibody Western Blot analysis of PDK2 expression in U-2 OS.)

WB (Western Blot) (Western Blot detection against Immunogen (35.31kD).)

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PDHK2, Monoclonal Antibody (Cat# AAA24307)

Full Name

PDHK2 [Pyruvate Dehydrogenase [Lipoamide]] Kinase Isozyme 2, Mitochondrial, Pyruvate Dehydrogenase Kinase Isoform 2, PDH Kinase 2, PDKII, PDK2) (AP)

Gene Names

PDK2; PDHK2; PDKII

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (EIF4G1 monoclonal antibody. Western Blot analysis of EIF4G1 expression in NIH/3T3.)

WB (Western Blot) (EIF4G1 monoclonal antibody, Western Blot analysis of EIF4G1 expression in HepG2.)

Application Data (Detection limit for recombinant GST tagged EIF4G1 is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to EIF4G1 on HeLa cell. [antibody concentration 10ug/ml].)

WB (Western Blot) (Western Blot analysis of EIF4G1 expression in transfected 293T cell line by EIF4G1 monoclonal antibody. Lane 1: EIF4G1 transfected lysate (70.95kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (EIF4G1 monoclonal antibody. Western Blot analysis of EIF4G1 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

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EIF4G1, Monoclonal Antibody (Cat# AAA24200)

Full Name

EIF4G1 (Eukaryotic Translation Initiation Factor 4 gamma 1, eIF-4-gamma 1, eIF-4G 1, eIF-4G1, p220, EIF4G1, EIF4F, EIF4G, EIF4GI) (AP)

Gene Names

EIF4G1; P220; EIF4F; EIF4G; EIF4GI; PARK18; EIF-4G1

Reactivity

Human, Mouse, Rat

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged GTF2H1 is ~1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GTF2H1 on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GTF2H1 on formalin-fixed paraffin-embedded human testis tissue.[antibody concentration 5ug/ml])

WB (Western Blot) (Western Blot analysis of GTF2H1 expression in transfected 293T cell line by GTF2H1 monoclonal antibody. Lane 1: GTF2H1 transfected lysate (62kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (GTF2H1 monoclonal antibody Western Blot analysis of GTF2H1 expression in Jurkat.)

WB (Western Blot) (GTF2H1 monoclonal antibody Western Blot analysis of GTF2H1 expression in Hela NE.)

WB (Western Blot) (Western Blot detection against Immunogen (86.02kD).)

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GTF2H1, Monoclonal Antibody (Cat# AAA24232)

Full Name

GTF2H1 (BTF2, General Transcription Factor IIH Subunit 1, Basic Transcription Factor 2 62kD Subunit, General Transcription Factor IIH Polypeptide 1, TFIIH Basal Transcription Factor Complex p62 Subunit) (AP)

Gene Names

GTF2H1; P62; BTF2; TFB1; TFIIH

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IF (Immunofluorescence) (IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100.)

WB (Western Blot) (Western blot analysis of 1) Mouse spleen tissue, 2) Rat spleen tissue, diluted at 1:3000.)

IF (Immunofluorescence) (Immunofluorescence analysis of Mouse-lung tissue. 1,EFHD1 Monoclonal Antibody(3G2)(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1,EFHD1 Monoclonal Antibody(3G2) was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1,EFHD1 Monoclonal Antibody(3G2) was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,EFHD1 Monoclonal Antibody(3G2) was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)

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EFHD1, Monoclonal Antibody (Cat# AAA23993)

Full Name

EFHD1 (5C10) Mouse mAb

Gene Names

EFHD1; SWS2; MST133; PP3051; MSTP133

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, ICC, IF

Purity

Affinity purified

Pricing

$565/0.1 mL $1,070/2x0.1 mL $1,545/3x0.1 mL $1,900/5x0.1 mL $1,995/4x0.1 mL

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WB (Western Blot) (SPP1 monoclonal antibody (M08), clone 1E6. Western Blot analysis of SPP1 expression in NIH/3T3.)

Application Data (Detection limit for recombinant GST tagged SPP1 is approximately 1ng/ml as a capture antibody.)

WB (Western Blot) (SPP1 monoclonal antibody (M08), clone 1E6. Western Blot analysis of SPP1 expression in HepG2.)

WB (Western Blot) (SPP1 monoclonal antibody (M08), clone 1E6. Western Blot analysis of SPP1 expression in COLO 320 HSR.)

WB (Western Blot) (SPP1 monoclonal antibody (M08), clone 1E6 Western Blot analysis of SPP1 expression in Hela S3 NE.)

WB (Western Blot) (SPP1 monoclonal antibody (M08), clone 1E6. Western Blot analysis of SPP1 expression in human stomach.)

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SPP1, Monoclonal Antibody (Cat# AAA26037)

Full Name

SPP1 (Secreted Phosphoprotein 1, BNSP, BSPI, ETA-1, MGC110940, OPN) (APC)

Gene Names

SPP1; OPN; BNSP; BSPI; ETA-1

Applications

WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

What are Monoclonal Antibodies?

Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.

This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.

AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.

What Are The Uses of Monoclonal Antibodies

Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.

ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.

Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.

Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.

Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.

How Monoclonal Antibodies Are Used as Medicine?

Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).

Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:

• Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.


• Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.


• Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.

Why Buy Monoclonal Antibodies From Us?

At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.

1. Validated for Versatile Applications

The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).

2. Wide Selection & Specialized Options

We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.

3. High-Quality Proteins

Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.

Frequently Asked Questions

1. Are your monoclonal antibodies validated for specific applications?

Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.

2. How do I choose the right monoclonal antibody for my application?

Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.

3. How quickly can I receive my order?

Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.