Monoclonal Antibodies

Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.

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IF (Immunofluorescence) (IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100.)

WB (Western Blot) (Western blot analysis of 1) Mouse spleen tissue, 2) Rat spleen tissue, diluted at 1:3000.)

IF (Immunofluorescence) (Immunofluorescence analysis of Mouse-lung tissue. 1,EFHD1 Monoclonal Antibody(3G2)(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1,EFHD1 Monoclonal Antibody(3G2) was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1,EFHD1 Monoclonal Antibody(3G2) was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1,EFHD1 Monoclonal Antibody(3G2) was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)

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EFHD1, Monoclonal Antibody (Cat# AAA23993)

Full Name

EFHD1 (5C10) Mouse mAb

Gene Names

EFHD1; SWS2; MST133; PP3051; MSTP133

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, ICC, IF

Purity

Affinity purified

Pricing

$565/0.1 mL $1,070/2x0.1 mL $1,545/3x0.1 mL $1,900/5x0.1 mL $1,995/4x0.1 mL

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WB (Western Blot) (DCPS monoclonal antibody. Western Blot analysis of DCPS expression in PC-12.)

Application Data (Detection limit for recombinant GST tagged DCPS is ~3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to DCPS on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to DCPS on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1.5ug/ml])

WB (Western Blot) (DCPS monoclonal antibody Western Blot analysis of DCPS expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (63.18kD).)

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DCPS, Monoclonal Antibody (Cat# AAA24777)

Full Name

DCPS (DCS1, HINT5, m7GpppX Diphosphatase, DCS-1, Hint-related 7meGMP-directed Hydrolase, Histidine Triad Protein Member 5, Scavenger mRNA-decapping Enzyme DcpS, HSPC015) (Biotin)

Gene Names

DCPS; ARS; DCS1; HSL1; HINT5; HINT-5; HSPC015

Reactivity

Human, Rat

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of CTNS expression in PC-12 using AAA14800.)

WB (Western Blot) (Western Blot detection against immunogen using AAA14800 (36.74kD).)

WB (Western Blot) (Western Blot analysis of CTNS expression in Raw 264.7 using AAA14800.)

WB (Western Blot) (Western Blot analysis of CTNS expression in HepG2 using AAA14800 .)

WB (Western Blot) (Western Blot analysis of CTNS expression in human liver using AAA14800.)

Application Data (Detection limit is ~0.3ng/ml using AAA14800 as a capture antibody.)

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Cystinosin, Monoclonal Antibody (Cat# AAA14800)

Full Name

Cystinosin (CTNS)

Gene Names

Ctns; AI195360; AW049661

Reactivity

Human, Mouse, Rat

Applications

EL/EIA, WB

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

$820/0.1 mg $1,565/2x0.1 mg $2,270/3x0.1 mg $2,940/4x0.1 mg $3,380/5x0.1 mg

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Application Data (Detection limit for recombinant GST tagged S100A4 is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to S100A4 on HeLa cell. [antibody concentration 15ug/ml])

WB (Western Blot) (Western Blot analysis of S100A4 expression in transfected 293T cell line by S100A4 monoclonal antibody. Lane 1: S100A4 transfected lysate (11.7kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (S100A4 monoclonal antibody Western Blot analysis of S100A4 expression in NIH/3T3.)

WB (Western Blot) (S100A4 monoclonal antibody Western Blot analysis of S100A4 expression in Hela.)

WB (Western Blot) (Western Blot detection against Immunogen (37.22kD))

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S100A4, Monoclonal Antibody (Cat# AAA25828)

Full Name

S100A4 (Protein S100-A4, Calvasculin, Metastasin, Placental Calcium-binding Protein, Protein Mts1, S100 Calcium-binding Protein A4, CAPL, MTS1) (PE)

Gene Names

S100A4; 42A; 18A2; CAPL; FSP1; MTS1; P9KA; PEL98

Reactivity

Human, Mouse

Applications

EIA, IF, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data

IHC (Immunohistochemistry) (Immunoperoxidase of paraffin-embedded human uterine cervix using 126835 (0.5ug/ml))

WB (Western Blot) (Western Blot analysis of FKBP4 expression in Raw 264.7 using 126835.)

WB (Western Blot) (Western Blot analysis of FKBP4 expression in PC-12 using 126835.)

WB (Western Blot) (Western Blot analysis of FKBP4 expression in HeLa cells using 126835.)

WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)

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FKBP4, Monoclonal Antibody (Cat# AAA24803)

Full Name

FKBP4 (FK506-binding Protein 4, Peptidyl-prolyl cis-trans Isomerase FKBP4, PPIase FKBP4, Rotamase, HSP-binding Immunophilin, HBI, FKBP52 Protein, 52kD FK506-binding Protein, 52kD FKBP, FKBP-52, 59kD Immunophilin, FKBP59, p59 Protein, FKBP-4, FKBP52) (Biot

Gene Names

FKBP4; HBI; p52; Hsp56; FKBP51; FKBP52; FKBP59; PPIase

Reactivity

Human, Mouse, Rat

Applications

EIA, IHC, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged SPP1 is ~1ng/ml as a capture antibody.)

WB (Western Blot) (SPP1 monoclonal antibody. Western Blot analysis of SPP1 expression in NIH/3T3.)

WB (Western Blot) (SPP1 monoclonal antibody, Western Blot analysis of SPP1 expression in Hela NE.)

WB (Western Blot) (SPP1 monoclonal antibody. Western Blot analysis of SPP1 expression in COLO 320 HSR.)

WB (Western Blot) (SPP1 monoclonal antibody. Western Blot analysis of SPP1 expression in HeLa.)

WB (Western Blot) (SPP1 monoclonal antibody. Western Blot analysis of SPP1 expression in human stomach.)

WB (Western Blot) (Western Blot detection against Immunogen (59.11kD).)

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Osteopontin, Monoclonal Antibody (Cat# AAA24298)

Full Name

Osteopontin (Bone Sialoprotein 1, Nephropontin, Secreted Phosphoprotein 1, SPP-1, Urinary Stone Protein, Uropontin, SPP1, BNSP, OPN, PSEC0156, MGC110940) (AP)

Gene Names

SPP1; OPN; BNSP; BSPI; ETA-1

Reactivity

Human, Mouse

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Detection of human CD247/CD3Z by western blot. Samples: Whole cell lysate (10 ug) from Jurkat, U2OS, SR, HEK293T, and MOLT-4 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD247/CD3Z recombinant monoclonal antibody [BL-336-1B2] (AAA23794 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)

Simple Western (Detection of human CD247/CD3Z by Simple Western. Samples: Whole cell lysate (2.0 mg/mL) from Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD247/CD3Z recombinant monoclonal antibody [BL-336-1B2] (AAA23794) used at 1:10, 1:50, 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram.)

IP (Immunoprecipitation) (Detection of human CD247/CD3Z by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 10% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD247/CD3Z recombinant monoclonal antibody [BL-336-1B2] (AAA23794 lot 3) used for IP at 20 ul/mg lysate. CD247/CD3Z was also immunoprecipitated by a previous lot of this antibody (AAA23794 lot 2). For blotting immunoprecipitated CD247/CD3Z, AAA23794 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)

IHC (Immunohistochemistry) (Detection of human CD247/CD3Z (red) by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Rabbit anti-CD247/CD3Z recombinant monoclonal antibody [BL-336-1B2] (AAA23794 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI (blue).)

IHC (Immunohistochemistry) (Detection of human CD247/CD3Z in FFPE tonsil by immunohistochemistry. Antibody: Rabbit anti-CD247/CD3Z recombinant monoclonal antibody [BL-336-1B2] (AAA23794 lot 3). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

IHC (Immunohistochemistry) (Detection of human CD247/CD3Z in FFPE appendix by immunohistochemistry. Antibody: Rabbit anti-CD247/CD3Z recombinant monoclonal antibody [BL-336-1B2] (AAA23794 lot 3). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

FCM (Flow Cytometry) (Detection of human CD247/CD3Z by flow cytometry. Sample: Jurkat cells were fixed in 4% formaldehyde and permeabilized with 90% methanol. Antibody: 1E6 cells were incubated with 0.5 ul of rabbit anti-CD247/CD3Z recombinant monoclonal antibody [BL-336-1B2] (AAA23794 lot 1) (red shaded) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

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CD247/CD3Z, Monoclonal Recombinant Antibody (Cat# AAA23794)

Full Name

Rabbit anti-CD247/CD3Z Recombinant Monoclonal Antibody [BL-336-1B2]

Gene Names

CD247; T3Z; CD3H; CD3Q; CD3Z; TCRZ; CD3-ZETA

Reactivity

Human

Applications

WB, IP, IHC, IHC-IF, FC/FACS, SW-Size

Purity

Purified

Pricing

$295/0.01 mL $680/0.1 mL $980/5x0.01 mL $1,300/2x0.1 mL $1,890/3x0.1 mL $2,440/4x0.1 mL $2,755/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged SMN2 is ~0.3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SMN2 on HeLa cell. [antibody concentration 1-10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SMN2 on formalin-fixed paraffin-embedded human heart tissue. [antibody concentration 1-10ug/ml].)

WB (Western Blot) (SMN2 monoclonal antibody, Western Blot analysis of SMN2 expression in IMR-32.)

WB (Western Blot) (SMN2 monoclonal antibody. Western Blot analysis of SMN2 expression in human colon.)

WB (Western Blot) (Western Blot detection against Immunogen (57.13kD).)

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SMN2, Monoclonal Antibody (Cat# AAA25254)

Full Name

SMN2 (Survival Motor Neuron Protein, Component of Gems 1, SMN1, Gemin-1, SMN, SMNT, SMNC, FLJ76644, MGC20996, MGC5208) (FITC)

Gene Names

SMN2; SMNC; BCD541; GEMIN1; TDRD16B; C-BCD541

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (CDX4 monoclonal antibody. Western Blot analysis of CDX4 expression in NIH/3T3.)

Application Data (Detection limit for recombinant GST tagged CDX4 is ~0.03ng/ml as a capture antibody.)

WB (Western Blot) (CDX4 monoclonal antibody. Western Blot analysis of CDX4 expression in Raw 264.7.)

WB (Western Blot) (CDX4 monoclonal antibody. Western Blot analysis of CDX4 expression in PC-12.)

WB (Western Blot) (CDX4 monoclonal antibody Western Blot analysis of CDX4 expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

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CDX4, Monoclonal Antibody (Cat# AAA24758)

Full Name

CDX4 (Homeobox Protein CDX-4, Caudal-type Homeobox Protein 4) (Biotin)

Reactivity

Human, Mouse, Rat

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (MGC21874 monoclonal antibody. Western Blot analysis of MGC21874 expression in Raw 264.7.)

WB (Western Blot) (Western Blot detection against Immunogen (38.1kD).)

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TADA2B, Monoclonal Antibody (Cat# AAA24274)

Full Name

TADA2B (Transcriptional Adapter 2-beta, ADA2-like Protein beta, ADA2-beta, ADA2B, MGC21874) (AP)

Gene Names

TADA2B; ADA2B; ADA2(beta)

Reactivity

Human, Mouse, Rat

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data

Application Data

Application Data

Application Data

Application Data

IHC (Immunohistochemistry) (Immunohistochemistry (FFPE) analysis of human tonsil stained with, biotinylated anti-mouse IgG and Streptavidin Alkaline Phosphatase (red). Nuclei were counterstained with hematoxylin.)

IHC (Immunohistochemistry) (immunohistochemical analysis of an acetone fixed, frozen human colon section using.)

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CD45, Monoclonal Antibody (Cat# AAA26838)

Full Name

CD45 (CD45 Antigen, B220, GP180, Leukocyte Common Antigen, LCA, L-CA, LY5, LY-5, Protein Tyrosine Phosphatase Receptor Type C, Protein Tyrosine Phosphatase Receptor Type C Polypeptide, PTPRC, T200, T200 Glycoprotein) (MaxLight 750)

Gene Names

PTPRC; LCA; LY5; B220; CD45; L-CA; T200; CD45R; GP180

Reactivity

Equine, Human

Applications

FC/FACS, IHC, IP

Purity

Purified by Protein A Affinity Chromatography

Pricing

$955/0.1 mL $1,845/2x0.1 mL $2,675/3x0.1 mL $3,460/4x0.1 mL $4,015/5x0.1 mL

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WB (Western Blot) (RIPK2 monoclonal antibody (M02), clone 6F7. Western Blot analysis of RIPK2 expression in PC-12.)

WB (Western Blot) (RIPK2 monoclonal antibody (M02), clone 6F7. Western Blot analysis of RIPK2 expression in NIH/3T3.)

Application Data (Detection limit for recombinant GST tagged RIPK2 is approximately 0.1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RIPK2 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 1.2 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RIPK2 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 1.2 ug/ml])

WB (Western Blot) (RIPK2 monoclonal antibody (M02), clone 6F7 Western Blot analysis of RIPK2 expression in HeLa.)

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RIPK2, Monoclonal Antibody (Cat# AAA26072)

Full Name

RIPK2 (Receptor-Interacting Serine-Threonine Kinase 2, CARD3, CARDIAK, CCK, GIG30, RICK, RIP2) (APC)

Gene Names

RIPK2; CCK; RICK; RIP2; CARD3; GIG30; CARDIAK

Applications

IHC, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (FUSIP1 monoclonal antibody Western Blot analysis of FUSIP1 expression in Hela NE.)

Application Data (Detection limit for recombinant GST tagged FUSIP1 is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FUSIP1 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FUSIP1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of FUSIP1 expression in transfected 293T cell line by FUSIP1 monoclonal antibody Lane 1: FUSIP1 transfected lysate (22.2kD).Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)

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FUSIP1, Monoclonal Antibody (Cat# AAA24038)

Full Name

FUSIP1 (SRSF10, FUSIP1, FUSIP2, SFRS13A, TASR, Serine/Arginine-rich Splicing Factor 10, 40kD SR-repressor Protein, FUS-interacting Serine-arginine-rich Protein 1, Splicing Factor SRp38, Splicing Factor, Arginine/Serine-rich 13A, TLS-associated Protein wit

Gene Names

SRSF10; NSSR; TASR; SRp38; TASR1; TASR2; FUSIP1; FUSIP2; SFRS13; SRrp40; SFRS13A

Reactivity

Human

Applications

EL/EIA, WB, IHC, IF

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

$820/0.1 mg $1,565/2x0.1 mg $2,270/3x0.1 mg $2,940/4x0.1 mg $3,380/5x0.1 mg

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WB (Western Blot) (TRIB2 monoclonal antibody Western Blot analysis of TRIB2 expression in Jurkat.)

WB (Western Blot) (Western blot analysis of TRIB2 over-expressed 293 cell line, cotransfected with TRIB2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TRIB2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged TRIB2 is ~0.03ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TRIB2 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of TRIB2 expression in transfected 293T cell line by TRIB2 monoclonal antibody. Lane 1: TRIB2 transfected lysate (38.8kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (36.01kD).)

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TRIB2, Monoclonal Antibody (Cat# AAA25573)

Full Name

TRIB2 (Tribbles Homolog 2, TRB2, TRB-2, C5FW, GS3955) (HRP)

Gene Names

TRIB2; C5FW; TRB2; GS3955

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Dilution: IF: 1:50-200 IHC 1:100-200)

Application Data (Dilution: IF: 1:50-200 IHC 1:100-200)

IHC (Immunohistochemistry-Paraffin) (Dilution: IF: 1:50-200 IHC 1:100-200)

Application Data (Dilution: IF: 1:50-200 IHC 1:100-200)

Application Data (Dilution: IF: 1:50-200 IHC 1:100-200)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 IHC 1:100-200)

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phospho-MLKL, Monoclonal Antibody (Cat# AAA28911)

Full Name

phospho-MLKL (S358) mouse Monoclonal Antibody (6F8)

Reactivity

Human

Applications

IHC-P, IF

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of Daudi cells using anti-HLA-DRA antibody (AAA19680).Overlay histogram showing Daudi cells stained with AAA19680 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-HLA-DRA Antibody (AAA19680, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of HLA-DRA using anti-HLA-DRA antibody (AAA19680).HLA-DRA was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-HLA-DRA Antibody (AAA19680) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of HLA-DRA using anti-HLA-DRA antibody (AAA19680).HLA-DRA was detected in a paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-HLA-DRA Antibody (AAA19680) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of HLA-DRA using anti-HLA-DRA antibody (AAA19680).HLA-DRA was detected in a paraffin-embedded section of human mammary infiltrate tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-HLA-DRA Antibody (AAA19680) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of HLA-DRA using anti-HLA-DRA antibody (AAA19680).HLA-DRA was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-HLA-DRA Antibody (AAA19680) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of HLA-DRA using anti-HLA-DRA antibody (AAA19680).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Raji whole cell lysates,Lane 2: human Daudi whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HLA-DRA antigen affinity purified monoclonal antibody (#AAA19680) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HLA-DRA at approximately 35-37 kDa. The expected band size for HLA-DRA is at 29 kDa.)

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HLA-DR/HLA-DRA, Monoclonal Antibody (Cat# AAA19680)

Full Name

Anti-HLA-DR/HLA-DRA Antibody Picoband (monoclonal, 8I10H1)

Gene Names

HLA-DRA; MLRW; HLA-DRA1

Reactivity

Human

Applications

WB, IHC, FC/FACS

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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Application Data (Detection limit for recombinant GST tagged YY1 is approximately 0.03ng/ml as a capture antibody.)

WB (Western Blot) (YY1 monoclonal antibody (M04), clone 2C5 Western Blot analysis of YY1 expression in Hela S3 NE (Cat # L013V3).)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to YY1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to YY1 on HeLa cell. [antibody concentration 10 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to YY1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 7 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to YY1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 7 ug/ml])

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YY1, Monoclonal Antibody (Cat# AAA26309)

Full Name

YY1 (YY1 Transcription Factor, DELTA, INO80S, NF-E1, UCRBP, YIN-YANG-1) (FITC)

Gene Names

YY1; DELTA; NF-E1; UCRBP; GADEVS; INO80S; YIN-YANG-1

Applications

IF, IHC, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged YY1 is approximately 0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to YY1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to YY1 on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (YY1 monoclonal antibody (M03), clone 4D2 Western Blot analysis of YY1 expression in Hela S3 NE (Cat # L013V3).)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to YY1 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to YY1 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 3 ug/ml])

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YY1, Monoclonal Antibody (Cat# AAA26574)

Full Name

YY1 (YY1 Transcription Factor, DELTA, INO80S, NF-E1, UCRBP, YIN-YANG-1) (PE)

Gene Names

YY1; DELTA; NF-E1; UCRBP; GADEVS; INO80S; YIN-YANG-1

Applications

IF, IHC, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western blot analysis of ABL2 over-expressed 293 cell line, cotransfected with ABL2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ABL2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between NCK1 and ABL2. HeLa cells were stained with NCK1 rabbit purified polyclonal 1:1200 and ABL2 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Application Data (Detection limit for recombinant GST tagged ABL2 is ~0.03ng/ml as a capture antibody.)

WB (Western Blot) (Western Blot analysis of ABL2 expression in transfected 293T cell line by ABL2 monoclonal antibody. Lane 1: ABL2 transfected lysate (126.7kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (ABL2 monoclonal antibody Western Blot analysis of ABL2 expression in Hela NE.)

WB (Western Blot) (Western Blot detection against Immunogen (37kD).)

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ABL2, Monoclonal Antibody (Cat# AAA25299)

Full Name

ABL2 (Tyrosine-protein Kinase ABL2, Abelson Murine Leukemia Viral Oncogene Homolog 2, Abelson-related Gene Protein, Tyrosine Kinase ARG, ABLL, ARG) (HRP)

Gene Names

ABL2; ARG; ABLL

Reactivity

Human

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to PDK2 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 2 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PDK2 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 2 ug/ml])

Application Data (Detection limit for recombinant GST tagged PDK2 is approximately 0.03ng/ml as a capture antibody.)

WB (Western Blot) (PDK2 monoclonal antibody (M02), clone 5F8. Western Blot analysis of PDK2 expression in A-431 (Cat # L015V1).)

WB (Western Blot) (PDK2 monoclonal antibody (M02), clone 5F8. Western Blot analysis of PDK2 expression in 293 (Cat # L026V1).)

WB (Western Blot) (PDK2 monoclonal antibody (M02), clone 5F8 Western Blot analysis of PDK2 expression in HeLa (Cat # L013V1).)

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PDK2, Monoclonal Antibody (Cat# AAA26329)

Full Name

PDK2 (Pyruvate Dehydrogenase Kinase, Isozyme 2, PDHK2) (FITC)

Gene Names

PDK2; PDHK2; PDKII

Applications

IHC, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (SCARA3 monoclonal antibody, Western Blot analysis of SCARA3 expression in IMR-32.)

Application Data (Detection limit for recombinant GST tagged SCARA3 is ~3ng/ml as a capture antibody.)

WB (Western Blot) (SCARA3 monoclonal antibody, Western Blot analysis of SCARA3 expression in NIH/3T3.)

WB (Western Blot) (SCARA3 monoclonal antibody. Western Blot analysis of SCARA3 expression in HepG2.)

WB (Western Blot) (SCARA3 monoclonal antibody. Western Blot analysis of SCARA3 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)

Compare (5 max)

SCARA3, Monoclonal Antibody (Cat# AAA25832)

Full Name

SCARA3 (Scavenger Receptor Class A Member 3, Cellular Stress Response Gene Protein , CSR, CSR1, APC7, MSLR1, MSRL1) (PE)

Gene Names

SCARA3; CSR; APC7; CSR1; MSLR1; MSRL1

Reactivity

Human, Mouse, Rat

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of MPP1 expression in transfected 293T cell line by MPP1 monoclonal antibody (M01), clone 1E11-1G11.Lane 1: MPP1 transfected lysate (Predicted MW: 52.3 KDa).Lane 2: Non-transfected lysate.)

IP (Immunoprecipitation) (Immunoprecipitation of MPP1 transfected lysate using anti-MPP1 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with MPP1 monoclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MPP1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MPP1 on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (MPP1 monoclonal antibody (M01), clone 1E11-1G11. Western Blot analysis of MPP1 expression in human placenta.)

Application Data (Detection limit for recombinant GST tagged MPP1 is approximately 1ng/ml as a capture antibody.)

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MPP1, Monoclonal Antibody (Cat# AAA26610)

Full Name

MPP1 (Membrane Protein, Palmitoylated 1, 55kD, AAG12, DXS552, DXS552E, EMP55, MRG1, PEMP) (PE)

Gene Names

MPP1; MRG1; PEMP; AAG12; EMP55; DXS552E

Applications

IF, IP, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged PAK1 is ~0.1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of PAK1 transfected lysate using anti-PAK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PAK1 monoclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PAK1 on HeLa cell. [antibody concentration 35ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PAK1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of PAK1 expression in transfected 293T cell line by PAK1 monoclonal antibody. Lane 1: PAK1 transfected lysate (Predicted MW: 49.6kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (PAK1 monoclonal antibody Western Blot analysis of PAK1 expression in HeLa NE.)

WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)

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PAK1, Monoclonal Antibody (Cat# AAA25779)

Full Name

PAK1 (Serine/Threonine-protein Kinase PAK 1, Alpha-PAK, p21-activated Kinase 1, PAK-1, p65-PAK, MGC130000, MGC130001) (PE)

Gene Names

PAK1; IDDMSSD; p65-PAK; PAKalpha; alpha-PAK

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged TP53 is approximately 1ng/ml as a capture antibody.)

WB (Western Blot) (TP53 monoclonal antibody (M01), clone 2C3 Western Blot analysis of TP53 expression in A-431.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TP53 on A-431 cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TP53 on A-431 cell. [antibody concentration 10 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TP53 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to TP53 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml])

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TP53, Monoclonal Antibody (Cat# AAA26040)

Full Name

TP53 (Tumor Protein p53, FLJ92943, LFS1, TRP53, p53) (APC)

Gene Names

TP53; P53; BCC7; LFS1; BMFS5; TRP53

Applications

IF, IHC, IP, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged HADHSC is ~0.03ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of HADH transfected lysate using anti-HADH monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HADH rabbit polyclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HADHSC on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HADHSC on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of HADH expression in transfected 293T cell line by HADHSC monoclonal antibody. Lane 1: HADH transfected lysate (34.3kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (HADHSC monoclonal antibody Western Blot analysis of HADHSC expression in HepG2.)

WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)

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HADHSC, Monoclonal Antibody (Cat# AAA24529)

Full Name

HADHSC (L-3-Hydroxyacyl Coenzyme A Dehydrogenase Short Chain, Hydroxyacyl-coenzyme A Dehydrogenase Mitochondrial, HAD, HADH, HADH1, HCDH, HHF4, Medium and Short Chain L-3-Hydroxyacyl-coenzyme A Dehydrogenase, M/SCHAD, MGC8392, Short Chain 3-Hydroxyacyl-Co

Gene Names

HADH; HAD; HCDH; HHF4; HADH1; SCHAD; HADHSC; MSCHAD

Reactivity

Human

Applications

EIA, IF, IHC, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged FGR is ~0.3ng/ml as a capture antibody.)

IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to FGR on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3ug/ml.)

WB (Western Blot) (Western Blot analysis of FGR expression in transfected 293T cell line by FGR monoclonal antibody. Lane 1: FGR transfected lysate (59.5kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (FGR monoclonal antibody. Western Blot analysis of FGR expression in Raw 264.7.)

WB (Western Blot) (FGR monoclonal antibody. Western Blot analysis of FGR expression in PC-12.)

WB (Western Blot) (FGR monoclonal antibody. Western Blot analysis of FGR expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)

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FGR, Monoclonal Antibody (Cat# AAA24210)

Full Name

FGR (Tyrosine-protein Kinase Fgr, Gardner-Rasheed Feline Sarcoma Viral (v-fgr) Oncogene Homolog, Proto-oncogene c-Fgr, p55-Fgr, p58-Fgr, p58c-Fgr, SRC2) (AP)

Gene Names

FGR; SRC2; c-fgr; c-src2; p55-Fgr; p58-Fgr; p55c-fgr; p58c-fgr

Reactivity

Human, Mouse, Rat

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western blot analysis of NDUFS4 over-expressed 293 cell line, cotransfected with NDUFS4 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NDUFS4 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged NDUFS4 is ~0.3ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NDUFS4 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1ug/ml])

WB (Western Blot) (Western Blot analysis of NDUFS4 expression in transfected 293T cell line by NDUFS4 monoclonal antibody. Lane 1: NDUFS4 transfected lysate (20.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (NDUFS4 monoclonal antibody Western Blot analysis of NDUFS4 expression in A-431.)

WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)

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NDUFS4, Monoclonal Antibody (Cat# AAA25763)

Full Name

NDUFS4 (NADH Dehydrogenase (Ubiquinone) Fe-S Protein 4, Mitochondrial, NADH-Ubiquinone Oxidoreductase 18kD Subunit, Complex I-18kD, CI-18kD, Complex I-AQDQ, CI-AQDQ) (PE)

Gene Names

NDUFS4; AQDQ; CI-18; CI-AQDQ; CI-18 kDa

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (CRYM monoclonal antibody, Western Blot analysis of CRYM expression in Jurkat.)

Application Data (Detection limit for recombinant GST tagged CRYM is ~0.03ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of CRYM transfected lysate using CRYM monoclonal antibody and Protein A Magnetic Bead and immunoblotted with CRYM rabbit polyclonal antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CRYM on formalin-fixed paraffin-embedded human skeletal muscle. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of CRYM expression in transfected 293T cell line by CRYM monoclonal antibody. Lane 1: CRYM transfected lysate (33.8kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

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CRYM, Monoclonal Antibody (Cat# AAA24768)

Full Name

CRYM (Thiomorpholine-carboxylate Dehydrogenase, Mu-crystallin Homolog, NADP-regulated Thyroid-hormone-binding Protein, Ketimine Reductase, THBP) (Biotin)

Gene Names

CRYM; THBP; DFNA40

Reactivity

Human

Applications

EIA, IHC, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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<< Previous Next >>

Application Data (Overlay histogram showing Hela cells stained with AAA18857 (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (10ug/1x10^6cells) for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-mouse IgG (H+L) at 1/200 dilution for 1 h at 4 degree C. Isotype control antibody (green line) was mouse IgG1 (10ug/1x10^6cells) used under the same conditions. Acquisition of >10,000 events was performed.)

Application Data (Overlay histogram showing U251 cells stained with AAA18857 (red line). The cells were fixed with 70% Ethylalcohol (18h) and then permeabilized with 0.3% Triton X-100 for 2 min. The cells were then incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by the antibody (10ug/1x10^6cells) for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-mouse IgG (H+L) at 1/200 dilution for 1 h at 4 degree C. Isotype control antibody (green line) was mouse IgG1 (10ug/1x10^6cells) used under the same conditions. Acquisition of >10,000 events was performed.)

IF (Immunofluorescence) (Immunofluorescent analysis of U251 cells using AAA18857 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L))

IF (Immunofluorescence) (Immunofluorescent analysis of PC-3 cells using AAA18857 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L))

IF (Immunofluorescence) (Immunofluorescent analysis of Hela cells using AAA18857 at a dilution of 1:100 and Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L))

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human melanoma using AAA18857 at dilution of 1:100.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human kidney tissue using AAA18857 at dilution of 1:100.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human tonsil tissue using AAA18857 at dilution of 1:100.)

WB (Western Blot) (Western Blot Positive WB detected in: U251 whole cell lysate, SH-Sy5y whole cell lysate All lanes: NES antibody at 3 ug/mlPredicted band size: 260 kDa Observed band size: 260 kDa )

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Nestin, Monoclonal Antibody (Cat# AAA18857)

Full Name

Mouse Anti-Nestin monoclonal Antibody

Gene Names

NES; Nbla00170

Applications

EIA, WB, IHC, IF, FC/FACS

Purity

>95%, Protein G Purified

Pricing

$305/0.05 mL $400/0.1 mL $750/2x0.1 mL $1,090/3x0.1 mL $1,400/4x0.1 mL $1,705/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of ATP6V1G2 expression in transfected 293T cell line by ATP6V1G2 monoclonal antibody. Lane 1: ATP6V1G2 transfected lysate (13.6kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (ATP6V1G2 monoclonal antibody Western Blot analysis of ATP6V1G2 expression in NIH/3T3.)

WB (Western Blot) (ATP6V1G2 monoclonal antibody Western Blot analysis of ATP6V1G2 expression in Raw 264.7.)

WB (Western Blot) (ATP6V1G2 monoclonal antibody, Western Blot analysis of ATP6V1G2 expression in HepG2.)

WB (Western Blot) (ATP6V1G2 monoclonal antibody. Western Blot analysis of ATP6V1G2 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (34.32kD).)

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ATP6V1G2, Monoclonal Antibody (Cat# AAA25032)

Full Name

ATP6V1G2 (V-type Proton ATPase Subunit G 2, V-ATPase Subunit G 2, V-ATPase 13kD Subunit 2, Vacuolar Proton Pump Subunit G 2, ATP6G, ATP6G2, NG38) (FITC)

Gene Names

ATP6V1G2; NG38; ATP6G; VMA10; ATP6G2

Reactivity

Human, Mouse, Rat

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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<< Previous Next >>

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between BAD and MAPK8 Mahlavu cells were stained with BAD rabbit purified polyclonal 1:1200 and anti-MAPK8 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between PXN and MAPK8 Huh7 cells were stained with PXN rabbit purified polyclonal 1:1200 and MAPK8 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between CRK and MAPK8 HeLa cells were stained with CRK rabbit purified polyclonal 1:1200 and MAPK8 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

WB (Western Blot) (Western blot analysis of MAPK8 over-expressed 293 cell line, cotransfected with MAPK8 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MAPK8 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged MAPK8 is 1ng/ml as a capture antibody.)

WB (Western Blot) (Western Blot analysis of MAPK8 expression in transfected 293T cell line by MAPK8 monoclonal antibody. Lane 1: MAPK8 transfected lysate (48.3kD). Lane 2: Non-transfected lysate.)

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MAPK8, Monoclonal Antibody (Cat# AAA24265)

Full Name

MAPK8 (JNK1, PRKM8, SAPK1, SAPK1C, Mitogen-activated Protein Kinase 8, MAP Kinase 8, MAPK 8, JNK-46, Stress-activated Protein Kinase 1c, Stress-activated Protein Kinase JNK1, c-Jun N-terminal Kinase 1) (AP)

Gene Names

MAPK8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2

Reactivity

Human

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (FOXA1 monoclonal antibody (M05), clone 3C1. Western Blot analysis of FOXA1 expression in HepG2 (Cat # L019V1).)

WB (Western Blot) (FOXA1 monoclonal antibody (M05), clone 3C1 Western Blot analysis of FOXA1 expression in MCF-7 (Cat # L046V1).)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA1 on HeLa cell. [antibody concentration 10 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FOXA1 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 1.5 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to FOXA1 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 1.5 ug/ml])

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FOXA1, Monoclonal Antibody (Cat# AAA26332)

Full Name

FOXA1 (Forkhead Box A1, HNF3A, MGC33105, TCF3A) (FITC)

Gene Names

FOXA1; HNF3A; TCF3A

Applications

EIA, IF, IHC, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Dilution: WB: 1:1000-5000 IHC: 1:500-1000 IF 1:200)

IF (Immunofluorescence) (Dilution: WB: 1:1000-5000 IHC: 1:500-1000 IF 1:200)

IF (Immunofluorescence) (Dilution: WB: 1:1000-5000 IHC: 1:500-1000 IF 1:200)

IF (Immunofluorescence) (Dilution: WB: 1:1000-5000 IHC: 1:500-1000 IF 1:200)

IHC (Immunohistochemistry-Paraffin) (Dilution: WB: 1:1000-5000 IHC: 1:500-1000 IF 1:200)

IHC (Immunohistochemistry-Paraffin) (Dilution: WB: 1:1000-5000 IHC: 1:500-1000 IF 1:200)

WB (Western Blot) (Dilution: WB: 1:1000-5000 IHC: 1:500-1000 IF 1:200)

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CYCS, Monoclonal Antibody (Cat# AAA28892)

Full Name

CYCS Monoclonal Antibody (4B10)

Gene Names

CYCS; CYC; HCS; THC4

Reactivity

Human, Mouse, Rat, Chicken

Applications

WB, IHC-P, IF

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (NMI monoclonal antibody Western Blot analysis of NMI expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)

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NMI, Monoclonal Antibody (Cat# AAA24291)

Full Name

NMI (N-myc-interactor, Nmi, N-myc and STAT Interactor) (AP)

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 200ug extracts of HeLa cells using 3ug SOX9 antibody . Western blot was performed from the immunoprecipitate using SOX9 antibody at a dilition of 1:1000.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using SOX9 antibody . Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse testis using SOX9 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon using SOX9 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat testis using SOX9 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using SOX9 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 3min.)

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SOX9, Monoclonal Antibody (Cat# AAA28384)

Full Name

SOX9 Rabbit mAb

Gene Names

SOX9; CMD1; SRA1; CMPD1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF, IP

Purity

Affinity purification

Pricing

$300/0.02 mL $500/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,780/4x0.1 mL $1,960/5x0.1 mL

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FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of A431 cells using anti-FEN1 antibody (AAA19686).Overlay histogram showing A431 cells stained with AAA19686 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-FEN1 Antibody (AAA19686, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 9. IF analysis of FEN1 using anti-FEN1 antibody (AAA19686).FEN1 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL mouse anti-FEN1 Antibody (AAA19686) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 8. IHC analysis of FEN1 using anti-FEN1 antibody (AAA19686).FEN1 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-FEN1 Antibody (AAA19686) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of FEN1 using anti-FEN1 antibody (AAA19686).FEN1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-FEN1 Antibody (AAA19686) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of FEN1 using anti-FEN1 antibody (AAA19686).FEN1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-FEN1 Antibody (AAA19686) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of FEN1 using anti-FEN1 antibody (AAA19686).FEN1 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinomas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-FEN1 Antibody (AAA19686) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of FEN1 using anti-FEN1 antibody (AAA19686).FEN1 was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-FEN1 Antibody (AAA19686) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of FEN1 using anti-FEN1 antibody (AAA19686).FEN1 was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-FEN1 Antibody (AAA19686) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of FEN1 using anti-FEN1 antibody (AAA19686).FEN1 was detected in a paraffin-embedded section of human bladder epithelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-FEN1 Antibody (AAA19686) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of FEN1 using anti-FEN1 antibody (AAA19686).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human Jurkat whole cell lysates,Lane 3: human MOLT-4 whole cell lysates,Lane 4: rat thymus tissue lysates,Lane 5: rat PC-12 whole cell lysates,Lane 6: mouse thymus tissue lysates,Lane 6: mouse RAW264.7 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-FEN1 antigen affinity purified monoclonal antibody (#AAA19686) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for FEN1 at approximately 48 kDa. The expected band size for FEN1 is at 48 kDa.)

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FEN1, Monoclonal Antibody (Cat# AAA19686)

Full Name

Anti-FEN1 Antibody Picoband (monoclonal, 6F3F2)

Gene Names

FEN1; MF1; RAD2; FEN-1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ICC, IF, FC/FACS

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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WB (Western Blot) (Western Blot detection against Immunogen (85.84kD).)

WB (Western Blot) (Western Blot detection against Immunogen (85.84kD).)

Application Data (Detection limit for recombinant GST tagged STIP1 is ~0.1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of STIP1 transfected lysate using STIP1 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with STIP1 rabbit polyclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to STIP1 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to STIP1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 0.3ug/ml].)

WB (Western Blot) (Western Blot analysis of STIP1 expression in transfected 293T cell line by STIP1 monoclonal antibody Lane 1: STIP1 transfected lysate (62.6kD). Lane 2: Non-transfected lysate.)

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STIP1, Monoclonal Antibody (Cat# AAA25265)

Full Name

STIP1 (Stress-induced-phosphoprotein 1, STI1, Hsc70/Hsp90-organizing Protein, Hop, P60, Renal Carcinoma Antigen NY-REN-11, STI1L, Transformation-sensitive Protein IEF SSP 3521, IEF-SSP-3521) (FITC)

Gene Names

STIP1; HOP; P60; STI1; STI1L; HEL-S-94n; IEF-SSP-3521

Reactivity

Human

Applications

EIA, IF, IHC, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (ALS2CR8 monoclonal antibody, Western Blot analysis of ALS2CR8 expression in Hela NE.)

WB (Western Blot) (Western Blot detection against Immunogen (36.89kD).)

WB (Western Blot) (Western blot analysis of ALS2CR8 over-expressed 293 cell line, cotransfected with ALS2CR8 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ALS2CR8 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged ALS2CR8 is ~0.3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ALS2CR8 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ALS2CR8 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 1ug/ml].)

WB (Western Blot) (Western Blot analysis of ALS2CR8 expression in transfected 293T cell line by ALS2CR8 monoclonal antibody. Lane 1: ALS2CR8 transfected lysate (79.8kD). Lane 2: Non-transfected lysate.)

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ALS2CR8, Monoclonal Antibody (Cat# AAA25024)

Full Name

ALS2CR8 (CARF, Amyotrophic Lateral Sclerosis 2 Chromosomal Region Candidate Gene 8 Protein, Calcium-response Factor, Testis Development Protein NYD-SP24, DKFZp667N246, FLJ12675, FLJ21579) (FITC)

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HDAC1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of HDAC1 expression in transfected 293T cell line by HDAC1 monoclonal antibody Lane 1: HDAC1 transfected lysate (55.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (HDAC1 monoclonal antibody Western Blot analysis of HDAC1 expression in NIH/3T3)

WB (Western Blot) (HDAC1 monoclonal antibody Western Blot analysis of HDAC1 expression in Raw 264.7)

WB (Western Blot) (HDAC1 monoclonal antibody Western Blot analysis of HDAC1 expression in Hela NE)

WB (Western Blot) (HDAC1 monoclonal antibody. Western Blot analysis of HDAC1 expression in PC-12)

WB (Western Blot) (Western Blot detection against Immunogen (78.76kD).)

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HDAC1, Monoclonal Antibody (Cat# AAA24531)

Full Name

HDAC1 (Histone Deacetylase 1, HD1, HD-1, HDAC1, HDAC-1, RPD3, RPD3L1, Reduced Potassium Dependency 3 Like-1) APC

Gene Names

HDAC1; HD1; RPD3; GON-10; RPD3L1

Reactivity

Human, Mouse, Rat

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western blot analysis of SGK over-expressed 293 cell line, cotransfected with SGK Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with SGK monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged SGK is ~1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SGK on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SGK on formalin-fixed paraffin-embedded human stomach tissue. [antibody concentration 5ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SGK on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of SGK expression in transfected 293T cell line by SGK monoclonal antibody. Lane 1: SGK transfected lysate (48.9kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (73.52kD).)

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SGK, Monoclonal Antibody (Cat# AAA25835)

Full Name

SGK (SGK1, Serine/Threonine-protein Kinase SGK1, Serum/Glucocorticoid-regulated Kinase 1) (PE)

Gene Names

SGK1; SGK

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Dilution: WB 1:1000-2000, IHC 1:100-200 IF 1:200)

Application Data (Dilution: WB 1:1000-2000, IHC 1:100-200 IF 1:200)

IF (Immunofluorescence) (Dilution: WB 1:1000-2000, IHC 1:100-200 IF 1:200)

IF (Immunofluorescence) (Dilution: WB 1:1000-2000, IHC 1:100-200 IF 1:200)

IF (Immunofluorescence) (Dilution: WB 1:1000-2000, IHC 1:100-200 IF 1:200)

IHC (Immunohistochemistry-Paraffin) (Dilution: WB 1:1000-2000, IHC 1:100-200 IF 1:200)

WB (Western Blot) (Dilution: WB 1:1000-2000, IHC 1:100-200 IF 1:200)

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Ubiquitin, Monoclonal Antibody (Cat# AAA28909)

Full Name

Ubiquitin mouse Monoclonal Antibody (5F1)

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, IF

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

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WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)

WB (Western Blot) (Western blot analysis of ASNA1 over-expressed 293 cell line, cotransfected with ASNA1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ASNA1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged ASNA1 is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ASNA1 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ASNA1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 0.5ug/ml])

WB (Western Blot) (Western Blot analysis of ASNA1 expression in transfected 293T cell line by ASNA1 monoclonal antibody. Lane 1: ASNA1 transfected lysate (38.8kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (ASNA1 monoclonal antibody. Western Blot analysis of ASNA1 expression in PC-12.)

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ASNA1, Monoclonal Antibody (Cat# AAA25326)

Full Name

ASNA1 (ATPase ASNA1, Arsenical Pump-driving ATPase, Arsenite-stimulated ATPase, Transmembrane Domain Recognition Complex 40kD ATPase Subunit, hARSA-I, hASNA-I, ARSA, TRC40) (HRP)

Gene Names

ASNA1; GET3; ARSA1; TRC40; ARSA-I; ASNA-I

Reactivity

Human, Rat

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (SMAD4 monoclonal antibody. Western Blot analysis of SMAD4 expression in IMR-32.)

WB (Western Blot) (Western Blot detection against Immunogen (86.46kD).)

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between CDKN1A and SMAD4 HeLa cells were stained with CDKN1A rabbit purified polyclonal 1:1200 and SMAD4 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Application Data (Detection limit for recombinant GST tagged SMAD4 is ~3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SMAD4 on HeLa cell. [antibody concentration 10ug/ml])

WB (Western Blot) (SMAD4 monoclonal antibody Western Blot analysis of SMAD4 expression in Hela NE.)

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SMAD4, Monoclonal Antibody (Cat# AAA25841)

Full Name

SMAD4 (Mothers Against Decapentaplegic Homolog 4, SMAD 4, Mothers Against DPP Homolog 4, Deletion Target In Pancreatic Carcinoma 4, MAD Homolog 4, SMAD Family Member 4, hSMAD4, DPC4, MADH4) (PE)

Gene Names

SMAD4; JIP; DPC4; MADH4; MYHRS

Reactivity

Human

Applications

EIA, IF, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western blot analysis of GPR3 over-expressed 293 cell line, cotransfected with GPR3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with GPR3 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged GPR3 is ~0.03ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GPR3 on formalin-fixed paraffin-embedded human transitional cell carcinoma tissue. [antibody concentration 1ug/ml].)

WB (Western Blot) (Western Blot analysis of GPR3 expression in transfected 293T cell line by GPR3 monoclonal antibody. Lane 1: GPR3 transfected lysate (35kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (GPR3 monoclonal antibody, Western Blot analysis of GPR3 expression in Jurkat.)

WB (Western Blot) (Western Blot detection against Immunogen (62.04kD).)

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GPR3, Monoclonal Antibody (Cat# AAA24818)

Full Name

GPR3 (G-protein Coupled Receptor 3, ACCA Orphan Receptor, ACCA) (Biotin)

Gene Names

GPR3; ACCA

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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ICC (Immunocytochemistry) (ICC staining PDPK1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining PDPK1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining PDPK1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PDPK1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PDPK1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PDPK1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PDPK1 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of PDPK1 on different lysates using anti-PDPK1 antibody at 1/1, 000 dilution. Positive control: Lane 1: 293T Lane 2: PC-12 Lane 3: HL-60)

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PDPK1, Monoclonal Antibody (Cat# AAA30194)

Full Name

PDPK1 Antibody

Gene Names

PDPK1; PDK1; PDPK2; PRO0461

Reactivity

Human, Mouse, Rat

Applications

WB, ICC, IF, IHC

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged SORD is ~0.1ng/ml as a capture antibody.)

IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to SORD on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1.2ug/ml].)

WB (Western Blot) (Western Blot analysis of SORD expression in transfected 293T cell line by SORD monoclonal antibody. Lane 1: SORD transfected lysate (38.165kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (SORD monoclonal antibody. Western Blot analysis of SORD expression in Raw 264.7.)

WB (Western Blot) (SORD monoclonal antibody, Western Blot analysis of SORD expression in Hela NE.)

WB (Western Blot) (SORD monoclonal antibody. Western Blot analysis of SORD expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (38.21kD).)

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SORD, Monoclonal Antibody (Cat# AAA25849)

Full Name

SORD (Sorbitol Dehydrogenase, L-iditol 2-dehydrogenase) (PE)

Gene Names

SORD; SORD1; HEL-S-95n

Reactivity

Human, Mouse, Rat

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged FOXA1 is approximately 0.03ng/ml as a capture antibody.)

WB (Western Blot) (FOXA1 monoclonal antibody (M02), clone 1B1 Western Blot analysis of FOXA1 expression in HepG2 (Cat # L019V1).)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA1 on HepG2 cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA1 on HepG2 cell. [antibody concentration 10 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FOXA1 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to FOXA1 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml])

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FOXA1, Monoclonal Antibody (Cat# AAA26614)

Full Name

FOXA1 (Forkhead Box A1, HNF3A, MGC33105, TCF3A) (PE)

Gene Names

FOXA1; HNF3A; TCF3A

Applications

IF, IHC, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged PGGT1B is ~1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PGGT1B on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PGGT1B on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1.2ug/ml])

WB (Western Blot) (PGGT1B monoclonal antibody Western Blot analysis of PGGT1B expression in PC-12.)

WB (Western Blot) (PGGT1B monoclonal antibody Western Blot analysis of PGGT1B expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (37.29kD).)

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PGGT1B, Monoclonal Antibody (Cat# AAA24309)

Full Name

PGGT1B (Geranylgeranyl Transferase Type-1 Subunit beta, Geranylgeranyl Transferase Type I Subunit beta, GGTase-I-beta, Type I Protein Geranyl-Geranyltransferase Subunit beta) (AP)

Reactivity

Human, Rat

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of Caco-2 cells using anti-SHP1/PTPN6 antibody (AAA19675).Overlay histogram showing Caco-2 cells stained with AAA19675 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-SHP1/PTPN6 Antibody (AAA19675, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of SHP1/PTPN6 using anti-SHP1/PTPN6 antibody (AAA19675).SHP1/PTPN6 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL mouse anti-SHP1/PTPN6 Antibody (AAA19675) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of SHP1/PTPN6 using anti-SHP1/PTPN6 antibody (AAA19675).SHP1/PTPN6 was detected in a paraffin-embedded section of human SM fatty carcinoma of the left kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-SHP1/PTPN6 Antibody (AAA19675) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of SHP1/PTPN6 using anti-SHP1/PTPN6 antibody (AAA19675).SHP1/PTPN6 was detected in a paraffin-embedded section of human serous adenocarcinoma of ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-SHP1/PTPN6 Antibody (AAA19675) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of SHP1/PTPN6 using anti-SHP1/PTPN6 antibody (AAA19675).SHP1/PTPN6 was detected in a paraffin-embedded section of human renal clear cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-SHP1/PTPN6 Antibody (AAA19675) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of SHP1/PTPN6 using anti-SHP1/PTPN6 antibody (AAA19675).SHP1/PTPN6 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinomas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-SHP1/PTPN6 Antibody (AAA19675) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of SHP1/PTPN6 using anti-SHP1/PTPN6 antibody (AAA19675).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Jurkat whole cell lysates,Lane 2: human Raji whole cell lysates,Lane 3: human Hela whole cell lysates,Lane 4: human HepG2 whole cell lysates,Lane 5: rat RH35 whole cell lysates,Lane 6: mouse HEPA1-6 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-SHP1/PTPN6 antigen affinity purified monoclonal antibody (#AAA19675) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SHP1/PTPN6 at approximately 68 kDa. The expected band size for SHP1/PTPN6 is at 68 kDa.)

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SHP1/PTPN6, Monoclonal Antibody (Cat# AAA19675)

Full Name

Anti-SHP1/PTPN6 Antibody Picoband (monoclonal, 8H11B10)

Gene Names

PTPN6; HCP; HCPH; SHP1; SHP-1; HPTP1C; PTP-1C; SHP-1L; SH-PTP1

Reactivity

Human, Mouse, Rat

Applications

FC/FACS, IF, IHC, ICC, WB

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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Application Data (Staining of mouse splenocytes with Rat anti Mouse CD40: RPE)

Application Data (Staining of mouse splenocytes with Rat anti Mouse CD40:FITC)

Application Data (Staining of mouse splenocytes with Rat anti Mouse CD40:RPE)

Application Data (Staining of mouse splenocytes cells with Rat anti Mouse CD40)

Application Data (Staining of mouse splenocytes with Rat anti Mouse CD40: Alexa Fluor 647)

Application Data (Staining of mouse splenocytes with Rat anti Mouse CD40: Low Endotoxin)

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CD40, Monoclonal Antibody (Cat# AAA11897)

Full Name

RAT ANTI MOUSE CD40:Low Endotoxin

Gene Names

Cd40; IGM; p50; Bp50; GP39; IMD3; TRAP; HIGM1; T-BAM; Tnfrsf5; AI326936

Applications

Immunohistochemistry, Flow Cytometry, Functional Assay

Pricing

$915/0.5 mg $1,755/2x0.5 mg $2,550/3x0.5 mg $3,300/4x0.5 mg $3,790/5x0.5 mg

What are Monoclonal Antibodies?

Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.

This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.

AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.

What Are The Uses of Monoclonal Antibodies

Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.

ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.

Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.

Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.

Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.

How Monoclonal Antibodies Are Used as Medicine?

Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).

Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:

• Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.


• Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.


• Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.

Why Buy Monoclonal Antibodies From Us?

At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.

1. Validated for Versatile Applications

The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).

2. Wide Selection & Specialized Options

We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.

3. High-Quality Proteins

Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.

Frequently Asked Questions

1. Are your monoclonal antibodies validated for specific applications?

Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.

2. How do I choose the right monoclonal antibody for my application?

Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.

3. How quickly can I receive my order?

Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.