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WB (Western Blot) (Western Blot detection against Immunogen (84.74kD).)
WB (Western Blot) (PHGDH monoclonal antibody. Western Blot analysis of PHGDH expression in human liver.)
IP (Immunoprecipitation) (Immunoprecipitation of PHGDH transfected lysate using PHGDH monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PHGDH rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PHGDH on HeLa cell. [antibody concentration 1-10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PHGDH on formalin-fixed paraffin-embedded human prostate. [antibody concentration 3ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PHGDH on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 1-10ug/ml])
WB (Western Blot) (Western Blot analysis of PHGDH expression in transfected 293T cell line by PHGDH monoclonal antibody. Lane 1: PHGDH transfected lysate (56.7kD). Lane 2: Non-transfected lysate.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ROD1 on HeLa cell. [antibody concentration 20ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ROD1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml])
WB (Western Blot) (ROD1 monoclonal antibody. Western Blot analysis of ROD1 expression in NIH/3T3)
WB (Western Blot) (ROD1 monoclonal antibody Western Blot analysis of ROD1 expression in Hela NE.)
WB (Western Blot) (ROD1 monoclonal antibody Western Blot analysis of ROD1 expression in PC-12)
WB (Western Blot) (Western Blot detection against Immunogen (36.63kD).)
Application Data (Detection limit for recombinant GST tagged HNRPM is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HNRPM on HepG2 cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HNRPM on formalin-fixed paraffin-embedded human thyroid nodular goiter. [antibody concentration 3ug/ml.)
WB (Western Blot) (HNRPM monoclonal antibody. Western Blot analysis of HNRPM expression in NIH/3T3.)
WB (Western Blot) (HNRPM monoclonal antibody Western Blot analysis of HNRPM expression in HepG2.)
WB (Western Blot) (HNRPM monoclonal antibody Western Blot analysis of HNRPM expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (36.3kD).)
WB (Western Blot) (MAP3K7IP1 monoclonal antibody Western Blot analysis of MAP3K7IP1 expression in PC-12.)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between HSPA1L and MAP3K7IP1 HeLa cells were stained with HSPA1L rabbit purified polyclonal 1:1200 and MAP3K7IP1 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Application Data (Detection limit for recombinant GST tagged MAP3K7IP1 is ~0.3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MAP3K7IP1 on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (MAP3K7IP1 monoclonal antibody Western Blot analysis of MAP3K7IP1 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (36.89kD).)
TAB1 (TGF-beta-activated Kinase 1 and MAP3K7-binding Protein 1, Mitogen-activated Protein Kinase Kinase Kinase 7-interacting Protein 1, TGF-beta-activated Kinase 1-binding Protein 1, TAK1-binding Protein 1, MAP3K7IP1) (HRP)
WB (Western Blot) (PPIE monoclonal antibody Western Blot analysis of PPIE expression in PC-12.)
WB (Western Blot) (PPIE monoclonal antibody Western Blot analysis of PPIE expression in Hela NE.)
Application Data (Detection limit for recombinant GST tagged PPIE is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PPIE on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of PPIE expression in transfected 293T cell line by PPIE monoclonal antibody. Lane 1: PPIE transfected lysate (33.4kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (PPIE monoclonal antibody Western Blot analysis of PPIE expression in NIH/3T3.)
WB (Western Blot) (PPIE monoclonal antibody Western Blot analysis of PPIE expression in Raw 264.7.)
WB (Western Blot) (FLJ23356 monoclonal antibody. Western Blot analysis of FLJ23356 expression in HeLa.)
WB (Western Blot) (FLJ23356 monoclonal antibody. Western Blot analysis of FLJ23356 expression in HepG2.)
Application Data (Detection limit for recombinant GST tagged FLJ23356 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FLJ23356 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of FLJ23356 expression in transfected 293T cell line by FLJ23356 monoclonal antibody. Lane 1: FLJ23356 transfected lysate (38.5kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western blot analysis of FLJ23356 over-expressed 293 cell line, cotransfected with FLJ23356 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with FLJ23356 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
WB (Western Blot) (SMURF1 monoclonal antibody, Western Blot analysis of SMURF1 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (37.55kD).)
Application Data (Detection limit for recombinant GST tagged SMURF1 is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of SMURF1 transfected lysate using SMURF1 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with SMURF1 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SMURF1 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SMURF1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 2ug/ml].)
WB (Western Blot) (Western Blot analysis of SMURF1 expression in transfected 293T cell line by SMURF1 monoclonal antibody. Lane 1: SMURF1 transfected lysate (83.4kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western blot analysis of ALPPL2 over-expressed 293 cell line, cotransfected with ALPPL2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ALPPL2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
IP (Immunoprecipitation) (Immunoprecipitation of ALPPL2 transfected lysate using ALPPL2 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with ALPPL2 rabbit polyclonal antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ALPPL2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of ALPPL2 expression in transfected 293T cell line by ALPPL2 monoclonal antibody Lane 1: ALPPL2 transfected lysate (57.5kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (ALPPL2 monoclonal antibody Western Blot analysis of ALPPL2 expression in A-431.)
WB (Western Blot) (Western Blot detection against Immunogen (35.64kD).)
WB (Western Blot) (DAAM1 monoclonal antibody Western Blot analysis of DAAM1 expression in A-431.)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between RHOA and DAAM1 HeLa cells were stained with RHOA rabbit purified polyclonal 1:1200 and DAAM1 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Application Data (Detection limit for recombinant GST tagged DAAM1 is ~1ng/ml as a capture antibody.)
WB (Western Blot) (Western Blot analysis of DAAM1 expression in transfected 293T cell line by DAAM1 monoclonal antibody. Lane 1: DAAM1 transfected lysate (122.3kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (DAAM1 monoclonal antibody Western Blot analysis of DAAM1 expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (38.21kD).)
FCM (Flow Cytometry) (Flow cytometry analysis of Hsp105alpha in Hep3B cell line, staining at 2-5ug for 1x106cells (red line). The secondary antibody used goat anti-mouse IgG Alexa fluor 488 conjugate. Isotype control antibody was mouse IgG (black line).)
ICC (Immunocytochemistry) (ICC/IF analysis of Hsp105alpha in Hep3B cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human Hsp105alpha antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
WB (Western Blot) (The cell lysates (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human Hsp105 alpha antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.Lane 1.: HeLa cell lysateLane 2.: HepG2 cell lysateLane 3.: NIH-3T3 cell lysateLane 4.: MCF7 cell lysateLane 5.: U-87 MG cell lysateLane 6.: A549 cell lysate)
WB (Western Blot) (HSPA1L monoclonal antibody Western Blot analysis of HSPA1L expression in PC-12.)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between MAP3K7 and HSPA1L HeLa cells were stained with MAP3K7 rabbit purified polyclonal 1:1200 and HSPA1L mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Application Data (Detection limit for recombinant GST tagged HSPA1L is ~0.1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HSPA1L on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml])
WB (Western Blot) (HSPA1L monoclonal antibody Western Blot analysis of HSPA1L expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (34.65kD).)
WB (Western Blot) (RUNX1 monoclonal antibody Western Blot analysis of RUNX1 expression in Hela NE.)
Application Data (Detection limit for recombinant GST tagged RUNX1 is ~1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RUNX1 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RUNX1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml].)
WB (Western Blot) (RUNX1 monoclonal antibody Western Blot analysis of RUNX1 expression in NIH/3T3.)
WB (Western Blot) (Western Blot detection against Immunogen (36.85kD).)
WB (Western Blot) (Western blot analysis of PGM3 over-expressed 293 cell line, cotransfected with PGM3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PGM3 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged PGM3 is ~3ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of PGM3 transfected lysate using PGM3 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PGM3 rabbit polyclonal antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PGM3 on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 2ug/ml])
WB (Western Blot) (Western Blot analysis of PGM3 expression in transfected 293T cell line by PGM3 monoclonal antibody. Lane 1: PGM3 transfected lysate (59.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (PGM3 monoclonal antibody Western Blot analysis of PGM3 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (85.36kD).)
WB (Western Blot) (CA1 monoclonal antibody, Western Blot analysis of CA1 expression in HeLa.)
Application Data (Detection limit for recombinant GST tagged CA1 is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of CA1 transfected lysate using 124177 and Protein A Magnetic Bead and immunoblotted with CA1 rabbit polyclonal antibody.)
WB (Western Blot) (Western Blot analysis of CA1 expression in transfected 293T cell line using 124177. Lane 1: CA1 transfected lysate (28.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot analysis of CA1 expression in human spleen using 124177)
WB (Western Blot) (Western Blot detection against Immunogen (54.45kD), using 124177.)
WB (Western Blot) (Western Blot analysis of LYPLA3 expression in NIH/3T3 using AAA24054.)
WB (Western Blot) (Western Blot analysis of PLA2G15 expression in transfected 293T cell line using AAA24054. Lane 1: PLA2G15 transfected lysate (46.7kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot analysis of LYPLA3 expression in Raw 264.7 using AAA24054.)
WB (Western Blot) (Western Blot analysis of LYPLA3 expression in PC-12 using AAA24054.)
WB (Western Blot) (Western Blot analysis of LYPLA3 expression in HeLa using AAA24054.)
WB (Western Blot) (Western Blot detection against Immunogen using AAA24054 (37kD) .)
WB (Western Blot) (Western blot analysis of GATA2 over-expressed 293 cell line, cotransfected with GATA2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with GATA2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged GATA2 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GATA2 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of GATA2 expression in transfected 293T cell line by GATA2 monoclonal antibody. Lane 1: GATA2 transfected lysate (50.5kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (GATA2 monoclonal antibody, Western Blot analysis of GATA2 expression in HeLa NE.)
WB (Western Blot) (Western Blot detection against Immunogen (36.96kD).)
WB (Western Blot) (TIMM9 monoclonal antibody. Western Blot analysis of TIMM9 expression in Raw 264.7.)
WB (Western Blot) (TIMM9 monoclonal antibody Western Blot analysis of TIMM9 expression in IMR-32.)
Application Data (Detection limit for recombinant GST tagged TIMM9 is ~0.1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TIMM9 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of TIMM9 expression in transfected 293T cell line by TIMM9 monoclonal antibody. Lane 1: TIMM9 transfected lysate (10.4kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (TIMM9 monoclonal antibody. Western Blot analysis of TIMM9 expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (35.9kD).)
IP (Immunoprecipitation) (Immunoprecipitation of USP33 transfected lysate using anti-USP33 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with USP33 rabbit polyclonal antibody.)
Application Data (Detection limit for recombinant GST tagged USP33 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to USP33 on HeLa cell. [antibody concentration 20ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to USP33 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of USP33 expression in transfected 293T cell line by USP33 monoclonal antibody Lane 1: USP33 transfected lysate (103.3kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
WB (Western Blot) (EIF2S1 monoclonal antibody, Western Blot analysis of EIF2S1 expression in HeLa.)
WB (Western Blot) (EIF2S1 monoclonal antibody. Western Blot analysis of EIF2S1 expression in Hela NE.)
WB (Western Blot) (Western blot analysis of EIF2S1 over-expressed 293 cell line, cotransfected with EIF2S1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with EIF2S1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged EIF2S1 is ~0.3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to EIF2S1 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of EIF2S1 expression in transfected 293T cell line by EIF2S1 monoclonal antibody. Lane 1: EIF2S1 transfected lysate (36.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (60.39kD).)
Application Data (Detection limit for recombinant GST tagged SMARCB1 is ~0.03ng/ml as a capture antibody.)
IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to SMARCB1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml])
WB (Western Blot) (SMARCB1 monoclonal antibody. Western Blot analysis of SMARCB1 expression in NIH/3T3.)
WB (Western Blot) (SMARCB1 monoclonal antibody. Western Blot analysis of SMARCB1 expression in Raw 264.7.)
WB (Western Blot) (SMARCB1 monoclonal antibody, Western Blot analysis of SMARCB1 expression in Hela NE.)
WB (Western Blot) (SMARCB1 monoclonal antibody, Western Blot analysis of SMARCB1 expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
WB (Western Blot) (AKAP8 monoclonal antibody (Western Blot analysis of AKAP8 expression in HeLa NE)
IP (Immunoprecipitation) (Immunoprecipitation of AKAP8 transfected lysate using AKAP8 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with AKAP8 monoclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to AKAP8 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to AKAP8 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of AKAP8 expression in transfected 293T cell line by AKAP8 monoclonal antibody Lane 1: AKAP8 transfected lysate (76.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (38.43kD).)
WB (Western Blot) (DDX56 monoclonal antibody. Western Blot analysis of DDX56 expression in PC-12.)
WB (Western Blot) (DDX56 monoclonal antibody, Western Blot analysis of DDX56 expression in HeLa.)
Application Data (Detection limit for recombinant GST tagged DDX56 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to DDX56 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to DDX56 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of DDX56 expression in transfected 293T cell line by DDX56 monoclonal antibody. Lane 1: DDX56 transfected lysate (62kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (36.89kD).)
WB (Western Blot) (Western Blot analysis of TUBB2A expression in transfected 293T cell line by TUBB2A monoclonal antibody. Lane 1: TUBB2A transfected lysate (49.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (TUBB2A monoclonal antibody. Western Blot analysis of TUBB2A expression in NIH/3T3.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TUBB2A on formalin-fixed paraffin-embedded human spleen. [antibody concentration 1.5ug/ml])
WB (Western Blot) (TUBB2A monoclonal antibody. Western Blot analysis of TUBB2A expression in Jurkat.)
WB (Western Blot) (TUBB2A monoclonal antibody. Western Blot analysis of TUBB2A expression in Raw 264.7.)
WB (Western Blot) (TUBB2A monoclonal antibody. Western Blot analysis of TUBB2A expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (74.47kD).)
IF (Immunofluorescence) (Figure 2: Immunofluorescence staining of Megalin in the brush border of the proximal tubule (AAA14941))
IHC (Immunohistochemistry) (Figure 1: Staining (paraffin-embedded tissue-sections fixed in formalin) of Megalin in the brush border of the proximal tubule (AAA14941))
WB (Western Blot) (ACATE2 monoclonal antibody Western Blot analysis of ACATE2 expression in MCF-7.)
Application Data (Detection limit for recombinant GST tagged ACOT9 is ~3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ACOT9 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ACOT9 on formalin-fixed paraffin-embedded human heart. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of ACOT9 expression in transfected 293T cell line by ACATE2 monoclonal antibody. Lane 1: ACOT9 transfected lysate (24kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (49.43kD).)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between MAPK3 and RPS6KA2 HeLa cells were stained with anti-MAPK3 rabbit purified polyclonal 1:1200 and anti-RPS6KA2 mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
WB (Western Blot) (Western blot analysis of RPS6KA2 over-expressed 293 cell line, cotransfected with RPS6KA2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with RPS6KA2 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RPS6KA2 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RPS6KA2 on formalin-fixed paraffin-embedded human cerebral cortex. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of RPS6KA2 expression in transfected 293T cell line by RPS6KA2 monoclonal antibody. Lane 1: RPS6KA2 transfected lysate (83.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (RPS6KA2 monoclonal antibody, Western Blot analysis of RPS6KA2 expression in Hela NE.)
Application Data (Detection limit for recombinant GST tagged NODAL is ~0.3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NODAL on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NODAL on formalin-fixed paraffin-embedded human endometrium cancer. [antibody concentration 3ug/ml])
WB (Western Blot) (NODAL monoclonal antibody Western Blot analysis of NODAL expression in PC-12.)
WB (Western Blot) (NODAL monoclonal antibody Western Blot analysis of NODAL expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (33.66kD).)
Application Data (Detection limit for recombinant GST tagged NR3C1 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NR3C1 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of NR3C1 expression in transfected 293T cell line by NR3C1 monoclonal antibody. Lane 1: NR3C1 transfected lysate (85.7kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (NR3C1 monoclonal antibody, Western Blot analysis of NR3C1 expression in HeLa NE.)
WB (Western Blot) (NR3C1 monoclonal antibody. Western Blot analysis of NR3C1 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)
WB (Western Blot) (Western blot analysis of UGP2 over-expressed 293 cell line, cotransfected with UGP2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with UGP2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged UGP2 is ~0.1ng/ml as a capture antibody.)
WB (Western Blot) (Western Blot analysis of UGP2 expression in transfected 293T cell line by UGP2 monoclonal antibody (M01). Lane 1: UGP2 transfected lysate (56.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (UGP2 monoclonal antibody Western Blot analysis of UGP2 expression in NIH/3T3.)
WB (Western Blot) (UGP2 monoclonal antibody Western Blot analysis of UGP2 expression in Raw 264.7)
WB (Western Blot) (UGP2 monoclonal antibody Western Blot analysis of UGP2 expression in PC-12)
WB (Western Blot) (UGP2 monoclonal antibody Western Blot analysis of UGP2 expression in HeLa)
WB (Western Blot) (Western blot analysis of GSR over-expressed 293 cell line, cotransfected with GSR Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with GSR monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged GSR is ~0.03ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GSR on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 6ug/ml].)
WB (Western Blot) (Western Blot analysis of GSR expression in transfected 293T cell line by GSR monoclonal antibody. Lane 1: GSR transfected lysate (56.3kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (GSR monoclonal antibody Western Blot analysis of GSR expression in IMR-32.)
WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)
Application Data (Detection limit for recombinant GST tagged ADRM1 is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of ADRM1 transfected lysate using ADRM1 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with ADRM1 monoclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ADRM1 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of ADRM1 expression in transfected 293T cell line by ADRM1 monoclonal antibody Lane 1: ADRM1 transfected lysate (42.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (ADRM1 monoclonal antibody Western Blot analysis of ADRM1 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (69.01kD).)
Application Data (Proximity Ligation Analysis of protein-protein interactions between TP53 and MDM2 HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-MDM2 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
WB (Western Blot) (Western blot analysis of MDM2 over-expressed 293 cell line, cotransfected with MDM2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MDM2 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged MDM2 is ~0.03ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to MDM2 on formalin-fixed paraffin-embedded human cerebral cortex. [antibody concentration 1.5ug/ml])
WB (Western Blot) (Western Blot analysis of MDM2 expression in transfected 293T cell line by MDM2 monoclonal antibody. Lane 1: MDM2 transfected lysate (55.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TESK2 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TESK2 on HeLa cell. [antibody concentration 10 ug/ml])
Application Data (Detection limit for recombinant GST tagged TESK2 is approximately 0.1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TESK2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.7 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TESK2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.7 ug/ml])
WB (Western Blot) (Western Blot analysis of TESK2 expression in transfected 293T cell line by TESK2 monoclonal antibody (M08), clone 5G1.Lane 1: TESK2 transfected lysate (60.3 KDa).Lane 2: Non-transfected lysate.)
WB (Western Blot) (F3 monoclonal antibody, Western Blot analysis of F3 expression in A-431.)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between F7 and F3. HeLa cells were stained with F7 rabbit purified polyclonal 1:1200 and F3 mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex)
Application Data (Detection limit for recombinant GST tagged F3 is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of F3 transfected lysate using F3 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with F3 rabbit polyclonal antibody.)
WB (Western Blot) (Western Blot analysis of F3 expression in transfected 293T cell line by F3 monoclonal antibody. Lane 1: F3 transfected lysate (33.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)
Application Data (Detection limit for recombinant GST tagged RPS7 is ~1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RPS7 on HeLa cell. [antibody concentration 20ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RPS7 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml])
WB (Western Blot) (RPS7 monoclonal antibody Western Blot analysis of RPS7 expression in NIH/3T3.)
WB (Western Blot) (RPS7 monoclonal antibody. Western Blot analysis of RPS7 expression in Raw 264.7.)
WB (Western Blot) (RPS7 monoclonal antibody. Western Blot analysis of RPS7 expression in PC-12.)
WB (Western Blot) (RPS7 monoclonal antibody Western Blot analysis of RPS7 expression in HeLa.)
WB (Western Blot) (NEK11 monoclonal antibody. Western Blot analysis of NEK11 expression in A-431.)
WB (Western Blot) (NEK11 monoclonal antibody, Western Blot analysis of NEK11 expression in HL-60.)
Application Data (Detection limit for recombinant GST tagged NEK11 is 0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NEK11 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NEK11 on formalin-fixed paraffin-embedded human endometrium tissue. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of NEK11 expression in transfected 293T cell line by NEK11 monoclonal antibody. Lane 1: NEK11 transfected lysate (55.5kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (79.13kD).)
WB (Western Blot) (Western blot analysis of FOXA2 over-expressed 293 cell line, cotransfected with FOXA2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with FOXA2 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged FOXA2 is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of FOXA2 transfected lysate using FOXA2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with FOXA2 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA2 on HepG2 cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of FOXA2 expression in transfected 293T cell line by FOXA2 monoclonal antibody Lane 1: FOXA2 transfected lysate (48.3kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (FOXA2 monoclonal antibody Western Blot analysis of FOXA2 expression in HepG2.)
WB (Western Blot) (Western Blot detection against Immunogen (36.19kD).)
Application Data (Detection limit for recombinant GST tagged HADHSC is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of HADH transfected lysate using anti-HADH monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HADH rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HADHSC on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HADHSC on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of HADH expression in transfected 293T cell line by HADHSC monoclonal antibody. Lane 1: HADH transfected lysate (34.3kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (HADHSC monoclonal antibody Western Blot analysis of HADHSC expression in HepG2.)
WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)
HADHSC (L-3-Hydroxyacyl Coenzyme A Dehydrogenase Short Chain, Hydroxyacyl-coenzyme A Dehydrogenase Mitochondrial, HAD, HADH, HADH1, HCDH, HHF4, Medium and Short Chain L-3-Hydroxyacyl-coenzyme A Dehydrogenase, M/SCHAD, MGC8392, Short Chain 3-Hydroxyacyl-Co
Application Data (Detection limit for recombinant GST tagged CAMKK2 is ~1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of CAMKK2 transfected lysate using CAMKK2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with CAMKK2 rabbit polyclonal antibody.)
WB (Western Blot) (Western Blot analysis of CAMKK2 expression in transfected 293T cell line by CAMKK2 monoclonal antibody Lane 1: CAMKK2 transfected lysate (59.6kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (CAMKK2 monoclonal antibody Western Blot analysis of CAMKK2 expression in K-562.)
WB (Western Blot) (CAMKK2 monoclonal antibody Western Blot analysis of CAMKK2 expression in IMR-32)
WB (Western Blot) (Western Blot detection against Immunogen (40.3kD).)
WB (Western Blot) (Detection of human FOXQ1 by western blot. Samples: Whole cell lysate (50 ug) from Hep-G2, HEK293T, NCI-H520, MUTZ-3, and HT-29 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-FOXQ1 recombinant monoclonal antibody (AAA23877 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
IP (Immunoprecipitation) (Detection of human FOXQ1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from NCI-H520 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-FOXQ1 recombinant monoclonal antibody (AAA23877 lot 1) used for IP at 6 ul/mg lysate. FOXQ1 was also immunoprecipitated by a second antibody against a different epitope of FOXQ1 (BL-2269A-2C5). For blotting immunoprecipitated FOXQ1, AAA23877 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry) (Detection of human FOXQ1 by immunohistochemistry. Sample: FFPE section of human colon carcinoma. Antibody: Rabbit anti-FOXQ1 recombinant monoclonal antibody (AAA23877-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
IHC (Immunohistochemistry) (Detection of mouse FOXQ1 by immunohistochemistry. Sample: FFPE section of mouse bladder. Antibody: Rabbit anti-FOXQ1 recombinant monoclonal antibody (AAA23877-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry) (Detection of human FOXQ1 by immunocytochemistry. Sample: FFPE section of NCI-H520 cells. Antibody: Rabbit anti-FOXQ1 recombinant monoclonal antibody (AAA23877-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry) (Detection of human FOXQ1 (shaded) in HT-29 cells by flow cytometry. Antibody: Rabbit anti-FOXQ1 recombinant monoclonal antibody (AAA23877) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
WB (Western Blot) (Western Blot analysis of CTNNB1 expression in transfected 293T cell line by CTNNB1 monoclonal antibody. Lane 1: CTNNB1 transfected lysate (85.5kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between FLT1 and CTNNB1. Huh7 cells were stained with FLT1 rabbit purified polyclonal 1:1200 and CTNNB1 mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between GSK3B and CTNNB1. HeLa cells were stained with GSK3B rabbit purified polyclonal 1:1200 and CTNNB1 mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
WB (Western Blot) (Western blot analysis of CTNNB1 over-expressed 293 cell line, cotransfected with CTNNB1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CTNNB1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged CTNNB1 is 0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CTNNB1 on HeLa cell. [antibody concentration 10ug/ml].)
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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