Monoclonal Antibodies

Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.

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WB (Western Blot) (ASB10 monoclonal antibody. Western Blot analysis of ASB10 expression in NIH/3T3.)

Application Data (Detection limit for recombinant GST tagged ASB10 is ~0.1ng/ml as a capture antibody.)

WB (Western Blot) (ASB10 monoclonal antibody, Western Blot analysis of ASB10 expression in A-431.)

WB (Western Blot) (ASB10 monoclonal antibody. Western Blot analysis of ASB10 expression in Raw 264.7.)

WB (Western Blot) (ASB10 monoclonal antibody. Western Blot analysis of ASB10 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (37.77kD).)

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ASB10, Monoclonal Antibody (Cat# AAA25028)

Full Name

ASB10 (Ankyrin Repeat and SOCS Box Protein 10, ASB-10) (FITC)

Gene Names

ASB10; GLC1F

Reactivity

Human, Mouse, Rat

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western Blot detection against Immunogen (85.84kD).)

WB (Western Blot) (Western Blot detection against Immunogen (85.84kD).)

Application Data (Detection limit for recombinant GST tagged STIP1 is ~0.1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of STIP1 transfected lysate using STIP1 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with STIP1 rabbit polyclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to STIP1 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to STIP1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 0.3ug/ml].)

WB (Western Blot) (Western Blot analysis of STIP1 expression in transfected 293T cell line by STIP1 monoclonal antibody Lane 1: STIP1 transfected lysate (62.6kD). Lane 2: Non-transfected lysate.)

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STIP1, Monoclonal Antibody (Cat# AAA25559)

Full Name

STIP1 (Stress-induced-phosphoprotein 1, STI1, Hsc70/Hsp90-organizing Protein, Hop, P60, Renal Carcinoma Antigen NY-REN-11, STI1L, Transformation-sensitive Protein IEF SSP 3521, IEF-SSP-3521) (HRP)

Gene Names

STIP1; HOP; P60; STI1; STI1L; HEL-S-94n; IEF-SSP-3521

Reactivity

Human

Applications

EIA, IHC, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TOMM20 on HeLa cell. [antibody concentration 10ug/ml])

Application Data (Detection limit for recombinant GST tagged TOMM20 is ~0.1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase on formalin-fixed paraffin-embedded human small Intestine using 1346004 (3ug/ml).)

WB (Western Blot) (Western Blot analysis in transfected 293T cell line using 134604. Lane 1: 134604 transfected lysate (16.3kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot analysis in NIH/3T3 using 134604.)

WB (Western Blot) (Western Blot analysis of in PC-12 using 134604.)

WB (Western Blot) (Western Blot analysis in HeLa using 134604.)

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TOMM20, Monoclonal Antibody (Cat# AAA25569)

Full Name

TOMM20 (Mitochondrial Import Receptor Subunit TOM20 Homolog, TOM20, Mitochondrial 20kD Outer Membrane Protein, Outer Mitochondrial Membrane Receptor Tom20, KIAA0016, MAS20, MOM19)

Gene Names

TOMM20; MAS20; MOM19; TOM20

Reactivity

Human, Mouse, Rat

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged SP1 is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SP1 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 1ug/ml].)

WB (Western Blot) (SP1 monoclonal antibody. Western Blot analysis of SP1 expression in NIH/3T3.)

WB (Western Blot) (SP1 monoclonal antibody, Western Blot analysis of SP1 expression in Hela NE.)

WB (Western Blot) (Western Blot detection against Immunogen (38.21kD).)

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SP1, Monoclonal Antibody (Cat# AAA25851)

Full Name

SP1 (Transcription Factor Sp1, TSFP1) (PE)

Reactivity

Human, Mouse

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (SAV1 monoclonal antibody (M02), Western Blot analysis of SAV1 expression in Hela NE.)

Application Data (Detection limit for recombinant GST tagged SAV1 is ~0.1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of SAV1 transfected lysate using SAV1 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with rabbit polyclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SAV1 on HeLa cell. [antibody concentration 60ug/ml].)

WB (Western Blot) (SAV1 monoclonal antibody. Western Blot analysis of SAV1 expression in HepG2.)

WB (Western Blot) (Western Blot detection against Immunogen (35.35kD).)

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SAV1, Monoclonal Antibody (Cat# AAA24945)

Full Name

SAV1 (Protein Salvador Homolog 1, SAV, 45kD WW Domain Protein, WW45, hWW45, WWP4) (Biotin)

Gene Names

SAV1; SAV; WW45; WWP4

Reactivity

Human

Applications

Immunofluorescence, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Tissue: hepatocytes. Species: Rat. Fixation: Paraffin Embedded. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:200. Liver sections were paraffin embedded. First pictures in series show two hours after exposure to stress, the second shows the control. Courtesy of: G. Matic, University of Belgrade, Serbia.)

WB (Western Blot) (Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000. Secondary Antibody: HRP Goat Anti-Rat.)

ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Tissue: hepatocyte nuclei. Species: Rat. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:200. Liver sections were paraffin embedded. First pictures in series show two hours after exposure to stress, the second shows the control. Courtesy of: G. Matic, University of Belgrade, Serbia.)

WB (Western Blot) (Western Blot analysis of Bovine MDBK cell lysates showing detection of Hsp70 protein using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:1000.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. HSP70/HSC70 cells stained brown.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp70 Monoclonal Antibody, Clone BB70. Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp70 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x. Inflammatory cells. HSP70/HSC70 stained brown.)

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Hsp70/Hsc70, Monoclonal Antibody (Cat# AAA17784)

Full Name

Hsp70/Hsc70 Antibody: ATTO 390

Gene Names

HSPA2; HSP70

Reactivity

Human, Mouse, Rat, Sheep, Dog, Beluga, Bovine, Fish, Guinea pig, Scallop pig, Hamster, Rabbit, Chicken, Xenopus, Drosophila, Yeast

Applications

WB, IP, IHC

Pricing

$685/0.2 mg $1,310/2x0.2 mg $1,905/3x0.2 mg $2,460/4x0.2 mg $2,705/5x0.2 mg

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WB (Western Blot) (CDC2 monoclonal antibody (M04), clone 8F1. Western Blot analysis of CDC2 expression in PC-12 (Cat # L012V1).)

WB (Western Blot) (CDC2 monoclonal antibody (M04), clone 8F1. Western Blot analysis of CDC2 expression in NIH/3T3 (Cat # L018V1).)

Application Data (Detection limit for recombinant GST tagged CDC2 is approximately 0.1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CDC2 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 1.5 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CDC2 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 1.5 ug/ml])

WB (Western Blot) (CDC2 monoclonal antibody (M04), clone 8F1 Western Blot analysis of CDC2 expression in Hela S3 NE (Cat # L013V3).)

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CDC2, Monoclonal Antibody (Cat# AAA26553)

Full Name

CDC2 (Cell Division Cycle 2, G1 to S and G2 to M, CDC28A, CDK1, DKFZp686L20222, MGC111195) (PE)

Gene Names

CDK1; CDC2; CDC28A; P34CDC2

Applications

IHC, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD45RA:FITC (MCA88F))

Application Data (Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD45RA:BIOTIN (MCA88B))

Application Data (Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD45RA:AZIDE FREE (MCA88XZ))

Application Data (Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD45RA:ALEXA 647 (MCA88A647))

Application Data (Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD45RA: RPE - Alexa Fluor® 647 (MCA88P647))

IHC (Immunohistochemistry) (Staining of formalin fixed, paraffin embedded human tonsil with Mouse anti human CD45RA (MCAP88))

IHC (Immunohistochemistry) (Staining of formalin fixed, paraffin embedded human tonsil with Mouse anti Human CD45RA (MCAP88))

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CD45RA, Monoclonal Antibody (Cat# AAA26811)

Full Name

CD45RA (CD45 Antigen, B220, GP180, Leukocyte Common Antigen, LCA, L-CA, LY5, LY-5, Protein Tyrosine Phosphatase Receptor Type C Polypeptide, PTPRC, T200, T200 Glycoprotein) (MaxLight 550)

Reactivity

Human, Monkey

Applications

FC/FACS, IHC

Purity

Purified by protein G affinity chromatography from tissue culture supernatant.

Pricing

$1,115/0.1 mL $2,145/2x0.1 mL $3,115/3x0.1 mL $4,035/4x0.1 mL $4,710/5x0.1 mL

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WB (Western Blot) (SMAD4 monoclonal antibody. Western Blot analysis of SMAD4 expression in IMR-32.)

WB (Western Blot) (Western Blot detection against Immunogen (86.46kD).)

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between CDKN1A and SMAD4 HeLa cells were stained with CDKN1A rabbit purified polyclonal 1:1200 and SMAD4 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Application Data (Detection limit for recombinant GST tagged SMAD4 is ~3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SMAD4 on HeLa cell. [antibody concentration 10ug/ml])

WB (Western Blot) (SMAD4 monoclonal antibody Western Blot analysis of SMAD4 expression in Hela NE.)

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SMAD4, Monoclonal Antibody (Cat# AAA25545)

Full Name

SMAD4 (Mothers Against Decapentaplegic Homolog 4, SMAD 4, Mothers Against DPP Homolog 4, Deletion Target In Pancreatic Carcinoma 4, MAD Homolog 4, SMAD Family Member 4, hSMAD4, DPC4, MADH4) (HRP)

Gene Names

SMAD4; JIP; DPC4; MADH4; MYHRS

Reactivity

Human

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western Blot detection against Immunogen (36.89kD).)

Application Data (Detection limit for recombinant GST tagged RICTOR is ~0.03ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of RICTOR transfected lysate using RICTOR monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RICTOR rabbit polyclonal antibody)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RICTOR on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of RICTOR expression in transfected 293T cell line by RICTOR monoclonal antibody. Lane 1: RICTOR transfected lysate (29.8kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (RICTOR monoclonal antibody Western Blot analysis of RICTOR expression in Hela NE.)

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RICTOR, Monoclonal Antibody (Cat# AAA25816)

Full Name

RICTOR (Rapamycin-insensitive Companion of mTOR, AVO3 Homolog, hAVO3, KIAA1999, mAVO3, PIA) (PE)

Gene Names

RICTOR; PIA; AVO3; hAVO3

Reactivity

Human

Applications

EIA, IHC, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Detection of human HIF2-alpha by western blot. Samples: Whole cell lysate (50 ug) from HepG2 and 786-O cells treated with 200 uM CoCl2 (+) or mock treated (-). Antibody: Rabbit anti-HIF2-alpha recombinant monoclonal antibody [BL-95-1A2] (AAA23785 lot 3) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 75 seconds.)

IP (Immunoprecipitation) (Detection of human HIF2-alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hep-G2 cells with 200 uM CoCl2 (+) or mock treated (-). Antibodies: Rabbit anti-HIF2-alpha recombinant monoclonal antibody [BL-95-1A2] (AAA23785 lot 3) used for IP at 20 ul/mg lysate. HIF2-alpha was also immunoprecipitated by a previous lot of this antibody (AAA23785 lot 2). For blotting immunoprecipitated HIF2-alpha, AAA23785 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 75 seconds.)

IHC (Immunohistochemistry) (Detection of human HIF2-alpha in FFPE renal cell carcinoma by IHC. Antibody: Rabbit anti-HIF2-alpha recombinant monoclonal antibody [BL95-1A2] (AAA23785 lot 3). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry) (Detection of human HIF2-alpha by immunocytochemistry. Sample: Formaldehyde-fixed Hep-G2 cells untreated (left) and treated with CoCl2 (right). Antibody: Rabbit anti-HIF2-alpha recombinant monoclonal antibody [BL-95-1A2] (AAA23785 lot 2) used at 1:100. Secodnary: DyLight 594-conjugated goat anti-rabbit IgG used at 1:100. Counterstain: Phalloidin Alexa Fluor 488 conjugated (green).)

ICC (Immunocytochemistry) (Detection of human HIF2-alpha in FFPE Hep-G2 cells with (left) and without (right) cobalt treatment by ICC. Antibody: Rabbit anti-HIF2-alpha recombinant monoclonal antibody [BL95-1A2] (AAA23785 lot 3). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry) (Detection of human HIF2-alpha in FFPE 786-O cells by ICC. Antibody: Rabbit anti-HIF2-alpha recombinant monoclonal antibody [BL95-1A2] (AAA23785 lot 3). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ChIP (Chromatin Immunoprecipitation) (Localization of HIF2-alpha Binding Sites by ChIP-sequencing. Chromatin from subcutaneous human tumor 786-O cells was immunoprecipitated with anti-HIF2-alpha antibody AAA23785 and analyzed by DNA sequencing. The figure illustrates the peak distribution of HIF2-alpha binding within a 250 Kb region of chromosome 11 as detected using anti-HIF2-alpha antibody AAA23785. ChIP-seq validation performed by Active Motif, Carlsbad, CA.)

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HIF2-alpha, Monoclonal Recombinant Antibody (Cat# AAA23785)

Full Name

Rabbit anti-HIF2-alpha Recombinant Monoclonal Antibody [BL-95-1A2]

Gene Names

EPAS1; HLF; MOP2; ECYT4; HIF2A; PASD2; bHLHe73

Reactivity

Human

Applications

WB, IP, IHC, ICC, ICC-IF, ChIP-Seq

Purity

Purified

Pricing

$295/0.02 mL $480/0.1 mL $915/2x0.1 mL $980/5x0.02 mL $1,325/3x0.1 mL $1,705/4x0.1 mL $1,855/5x0.1 mL

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IHC (Immunohistchemistry) (Figure 6. IHC analysis of PP2A-alpha/PPP2CA using anti-PP2A-alpha/PPP2CA antibody (AAA19366).PP2A-alpha/PPP2CA was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PP2A-alpha/PPP2CA Antibody (AAA19366) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of PP2A-alpha/PPP2CA using anti-PP2A-alpha/PPP2CA antibody (AAA19366).PP2A-alpha/PPP2CA was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PP2A-alpha/PPP2CA Antibody (AAA19366) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of PP2A-alpha/PPP2CA using anti-PP2A-alpha/PPP2CA antibody (AAA19366).PP2A-alpha/PPP2CA was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml mouse anti-PP2A-alpha/PPP2CA Antibody (AAA19366) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

FCM (Flow Cytometry) (Figure 3. Flow Cytometry analysis of U937 cells using anti-PP2A-alpha/PPP2CA antibody (AAA19366).Overlay histogram showing U937 cells stained with AAA19366 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- PP2A-alpha/PPP2CA Antibody (AAA19366, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 2. IF analysis of PP2A-alpha/PPP2CA using anti- PP2A-alpha/PPP2CA antibody (AAA19366).PP2A-alpha/PPP2CA was detected in immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 4μg/mL mouse anti- PP2A-alpha/PPP2CA Antibody (AAA19366) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

WB (Western Blot) (Figure 1. Western blot analysis of PP2A-alpha/PPP2CA using anti-PP2A-alpha/PPP2CA antibody (AAA19366).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HELA whole cell lysatesLane 2: human HEPG2 whole cell lysatesLane 3: monkey COS-7 whole cell lysatesLane 4: rat PC-12 whole cell lysatesLane 5: mouse NIH/3T3 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- PP2A-alpha/PPP2CA antigen affinity purified monoclonal antibody (Catalog # AAA19366) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for PP2A-alpha/PPP2CA at approximately 36KD. The expected band size for PP2A-alpha/PPP2CA is at 36KD.)

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PP2A-alpha/PPP2CA, Monoclonal Antibody (Cat# AAA19366)

Full Name

Anti-PP2A-alpha/PPP2CA Antibody (monoclonal, 3B6)

Gene Names

PPP2CA; RP-C; PP2Ac; PP2CA; PP2Calpha

Reactivity

Human, Monkey, Mouse, Rat

Applications

WB, IHC-P, ICC, IF, FC/FACS/FCM

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NFKB1 on HeLa cell. [antibody concentration 10 ug/ml])

Application Data (Proximity Ligation Analysis of protein-protein interactions between HSP90AB1 and NFKB1 HeLa cells were stained with anti-HSP90AB1 rabbit purified polyclonal 1:1200 and anti-NFKB1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)

Application Data (Detection limit for recombinant GST tagged NFKB1 is approximately 3ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NFKB1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1.5 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NFKB1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1.5 ug/ml])

WB (Western Blot) (Western Blot analysis of NFKB1 expression in transfected 293T cell line by NFKB1 monoclonal antibody (M02), clone 4A11.Lane 1: NFKB1 transfected lysate (105.4 KDa).Lane 2: Non-transfected lysate.)

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NFKB1, Monoclonal Antibody (Cat# AAA26360)

Full Name

NFKB1 (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1, DKFZp686C01211, EBP-1, KBF1, MGC54151, NF-kappa-B, NFKB-p105, NFKB-p50, p105, p50) (FITC)

Gene Names

NFKB1; p50; KBF1; p105; EBP-1; NF-kB; CVID12; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B1

Applications

IF, IHC, WB, Cell

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western blot analysis of TCF19 over-expressed 293 cell line, cotransfected with TCF19 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TCF19 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged TCF19 is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TCF19 on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TCF19 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of TCF19 expression in transfected 293T cell line by TCF19 monoclonal antibody. Lane 1: TCF19 transfected lysate (37.2kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (TCF19 monoclonal antibody Western Blot analysis of TCF19 expression in Hela NE.)

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TCF19, Monoclonal Antibody (Cat# AAA24977)

Full Name

TCF19 (Transcription Factor 19, TCF-19, SC1, SC1-1, Transcription Factor SC1) (Biotin)

Gene Names

TCF19; SC1; TCF-19

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western blot analysis of CDC25C over-expressed 293 cell line, cotransfected with CDC25C Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CDC25C monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

IP (Immunoprecipitation) (Immunoprecipitation of CDC25C transfected lysate using CDC25C monoclonal antibody and Protein A Magnetic Bead and immunoblotted with CDC25C rabbit polyclonal antibody.)

Application Data (Detection limit for recombinant GST tagged CDC25C is ~0.1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CDC25C on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 6ug/ml].)

WB (Western Blot) (Western Blot analysis of CDC25C expression in transfected 293T cell line by CDC25C monoclonal antibody. Lane 1: CDC25C transfected lysate (53.312kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (CDC25C monoclonal antibody, Western Blot analysis of CDC25C expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (37.73kD).)

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CDC25C, Monoclonal Antibody (Cat# AAA24754)

Full Name

CDC25C (Cell Division Cycle 25 Homolog C, Cdc 25C, CDC 25, Dual Specificity Phosphatase Cdc25C, M-phase Inducer Phosphatase 3, MPIP3, PPP1R60) (Biotin)

Gene Names

CDC25C; CDC25; PPP1R60

Reactivity

Human

Applications

EIA, IHC, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (RUNX2 monoclonal antibody. Western Blot analysis of RUNX2 expression in SJCRH30.)

Application Data (Detection limit for recombinant GST tagged RUNX2 is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RUNX2 on U-2 OS cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RUNX2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml].)

WB (Western Blot) (RUNX2 monoclonal antibody, Western Blot analysis of RUNX2 expression in K-562.)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

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RUNX2, Monoclonal Antibody (Cat# AAA25531)

Full Name

RUNX2 (Runt-related Transcription Factor 2, Core-binding Factor Subunit alpha-1, CBF-alpha-1, Acute Myeloid Leukemia 3 Protein, Oncogene AML-3, Polyomavirus Enhancer-binding Protein 2 alpha A Subunit, PEBP2-alpha A, PEA2-alpha A, SL3-3 Enhancer Factor 1 a

Gene Names

RUNX2; CCD; AML3; CCD1; CLCD; OSF2; CBFA1; OSF-2; PEA2aA; PEBP2aA; CBF-alpha-1

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (CDK2 monoclonal antibody. Western Blot analysis of CDK2 expression in Jurkat.)

WB (Western Blot) (Western blot analysis of CDK2 over-expressed 293 cell line, cotransfected with CDK2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CDK2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged CDK2 is ~0.03ng/ml as a capture antibody.)

WB (Western Blot) (Western Blot analysis of CDK2 expression in transfected 293T cell line by CDK2 monoclonal antibody. Lane 1: CDK2 transfected lysate (33.9kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (CDK2 monoclonal antibody, Western Blot analysis of CDK2 expression in HL-60.)

WB (Western Blot) (Western Blot detection against Immunogen (35.42kD).)

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CDK2, Monoclonal Antibody (Cat# AAA25052)

Full Name

CDK2 (Cell Division Protein Kinase 2, p33 Protein Kinase) (FITC)

Gene Names

CDK2; CDKN2; p33(CDK2)

Reactivity

Human

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (FLJ20097 monoclonal antibody, Western Blot analysis of FLJ20097 expression in HeLa.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FLJ20097 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FLJ20097 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml].)

WB (Western Blot) (FLJ20097 monoclonal antibody. Western Blot analysis of FLJ20097 expression in NIH/3T3.)

WB (Western Blot) (FLJ20097 monoclonal antibody. Western Blot analysis of FLJ20097 expression in human pancreas.)

WB (Western Blot) (Western Blot detection against Immunogen (37.44kD).)

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CCDC132, Monoclonal Antibody (Cat# AAA24804)

Full Name

CCDC132 (Coiled-coil Domain-containing Protein 132, KIAA1861, DKFZp313I2429, FLJ20097, FLJ23581, KIAA1861, MGC176659) (Biotin)

Reactivity

Human, Mouse

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (RUNX2 monoclonal antibody. Western Blot analysis of RUNX2 expression in SJCRH30.)

Application Data (Detection limit for recombinant GST tagged RUNX2 is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RUNX2 on U-2 OS cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RUNX2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml].)

WB (Western Blot) (RUNX2 monoclonal antibody, Western Blot analysis of RUNX2 expression in K-562.)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

Compare (5 max)

RUNX2, Monoclonal Antibody (Cat# AAA25827)

Full Name

RUNX2 (Runt-related Transcription Factor 2, Core-binding Factor Subunit alpha-1, CBF-alpha-1, Acute Myeloid Leukemia 3 Protein, Oncogene AML-3, Polyomavirus Enhancer-binding Protein 2 alpha A Subunit, PEBP2-alpha A, PEA2-alpha A, SL3-3 Enhancer Factor 1 a

Gene Names

RUNX2; CCD; AML3; CCD1; CLCD; OSF2; CBFA1; OSF-2; PEA2aA; PEBP2aA; CBF-alpha-1

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (IRX3 monoclonal antibody (M05), clone 1D7. Western Blot analysis of IRX3 expression in PC-12 (Cat # L012V1).)

WB (Western Blot) (IRX3 monoclonal antibody (M05), clone 1D7. Western Blot analysis of IRX3 expression in NIH/3T3 (Cat # L018V1).)

Application Data (Detection limit for recombinant GST tagged IRX3 is approximately 1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to IRX3 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to IRX3 on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (IRX3 monoclonal antibody (M05), clone 1D7 Western Blot analysis of IRX3 expression in Hela S3 NE (Cat # L013V3).)

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IRX3, Monoclonal Antibody (Cat# AAA26335)

Full Name

IRX3 (Iroquois Homeobox 3, IRX-1) (FITC)

Gene Names

IRX3; IRX-1; IRXB1

Applications

IF, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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ICC (Immunocytochemistry) (ICC staining TRK fused gene in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining TRK fused gene in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining TRK fused gene in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TRK fused gene antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-TRK fused gene antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-TRK fused gene antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-TRK fused gene antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-TRK fused gene antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of TRK fused gene on different lysates using anti-TRK fused gene antibody at 1/5, 000 dilution. Positive control: Lane 1: 293T Lane 2: Hela)

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TRK Fused Gene, Monoclonal Antibody (Cat# AAA30204)

Full Name

TRK Fused Gene Antibody

Gene Names

TFG; TF6; HMSNP; SPG57; TRKT3

Reactivity

Human, Mouse, Rat

Applications

WB, ICC, IF, IHC, IP

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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WB (Western Blot) (Western blot analysis of FOXA1 over-expressed 293 cell line, cotransfected with FOXA1 Validated Chimera RNAi ((Lane 2) or non-transfected control (Lane 1). Blot probed with FOXA1 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged FOXA1 is ~0.03ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FOXA1 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of FOXA1 expression in transfected 293T cell line by FOXA1 monoclonal antibody Lane 1: FOXA1 transfected lysate (49.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (FOXA1 monoclonal antibody Western Blot analysis of FOXA1 expression in HepG2)

WB (Western Blot) (Western Blot detection against Immunogen (37.4kD).)

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FOXA1, Monoclonal Antibody (Cat# AAA24215)

Full Name

FOXA1 (Forkhead Box A1, Hepatocyte Nuclear Factor 3 alpha, HNF-3-alpha, HNF3A, HNF-3A, MGC33105, Transcription Factor 3A, TCF3A, TCF-3A) (AP)

Gene Names

FOXA1; HNF3A; TCF3A

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western blot analysis of CRKL over-expressed 293 cell line, cotransfected with CRKL Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CRKL monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

WB (Western Blot) (Western Blot analysis of CRKL expression in transfected 293T cell line by CRKL monoclonal antibody. Lane 1: CRKL transfected lysate (33.8kD). Lane 2: Non-transfected lysate.)

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between PTK2 and CRKL. Huh7 cells were stained with PTK2 rabbit purified polyclonal 1:600 and CRKL mouse monoclonal antibody 1:100. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex)

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between GAB1 and CRKL. HeLa cells were stained with GAB1 rabbit purified polyclonal 1:1200 and CRKL mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Application Data (Detection limit for recombinant GST tagged CRKL is ~0.3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CRKL on HeLa cell. [antibody concentration 10ug/ml].)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

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CRKL, Monoclonal Antibody (Cat# AAA24767)

Full Name

CRKL (Crk-like Protein) (Biotin)

Reactivity

Human

Applications

EIA, IF, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

WB (Western Blot) (Western Blot detection against Immunogen (37kD).)

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KHDRBS1, Monoclonal Antibody (Cat# AAA24252)

Full Name

KHDRBS1 (KH Domain-containing, RNA-binding, Signal Transduction-associated Protein 1, FLJ34027, GAP-associated Tyrosine Phosphoprotein p62, p21 Ras GTPase-activating Protein-associated p62, p62, p68, Src-associated in Mitosis 68kD Protein, Sam68) (AP)

Gene Names

KHDRBS1; p62; p68; Sam68

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between FGA and F2. HeLa cells were stained with FGA rabbit purified polyclonal 1:1200 and F2 mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

WB (Western Blot) (Western blot analysis of F2 over-expressed 293 cell line, cotransfected with F2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with F2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged F2 is ~1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of F2 transfected lysate using F2 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with F2 rabbit polyclonal antibody.)

WB (Western Blot) (Western Blot analysis of F2 expression in transfected 293T cell line by F2 monoclonal antibody. Lane 1: F2 transfected lysate (70kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

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F2, Monoclonal Antibody (Cat# AAA24797)

Full Name

F2 (Coagulation Factor II, Prothrombin) (Biotin)

Gene Names

F2; PT; THPH1; RPRGL2

Reactivity

Human

Applications

EIA, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (NUP62 monoclonal antibody. Western Blot analysis of NUP62 expression in NIH/3T3.)

Application Data (Detection limit for recombinant GST tagged NUP62 is ~1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NUP62 on HeLa cell. [antibody concentration 10ug/ml])

WB (Western Blot) (NUP62 monoclonal antibody Western Blot analysis of NUP62 expression in Hela NE.)

WB (Western Blot) (NUP62 monoclonal antibody. Western Blot analysis of NUP62 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)

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NUP62, Monoclonal Antibody (Cat# AAA24296)

Full Name

NUP62 (Nuclear Pore Glycoprotein p62, 62kD Nucleoporin, Nucleoporin Nup62) (AP)

Gene Names

NUP62; p62; IBSN; SNDI

Reactivity

Human, Mouse, Rat

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between CASP3 and HCLS1. HeLa cells were stained with anti-CASP3 rabbit purified polyclonal 1:1200 and anti-HCLS1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

WB (Western Blot) (Western blot analysis of HCLS1 over-expressed 293 cell line, cotransfected with HCLS1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with HCLS1 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged HCLS1 is ~0.1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HCLS1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of HCLS1 expression in transfected 293T cell line by HCLS1 monoclonal antibody. Lane 1: HCLS1 transfected lysate (54kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (HCLS1 monoclonal antibody Western Blot analysis of HCLS1 expression in K-562.)

WB (Western Blot) (Western Blot detection against Immunogen (35.64kD).)

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HCLS1, Monoclonal Antibody (Cat# AAA24825)

Full Name

HCLS1 (Hematopoietic Lineage Cell-specific Protein, Hematopoietic Cell-specific LYN Substrate 1, LckBP1, p75, HS1) (Biotin)

Gene Names

HCLS1; HS1; p75; CTTNL; lckBP1

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western blot analysis of DUSP6 over-expressed 293 cell line, cotransfected with DUSP6 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with DUSP6 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged DUSP6 is ~1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to DUSP6 on HeLa cell. [antibody concentration 10ug/ml.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to DUSP6 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of DUSP6 expression in transfected 293T cell line by DUSP6 monoclonal antibody. Lane 1: DUSP6 transfected lysate (42kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (67.65kD).)

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DUSP6, Monoclonal Antibody (Cat# AAA25082)

Full Name

DUSP6 (Dual Specificity Protein Phosphatase 6, Dual Specificity Protein Phosphatase PYST1, Mitogen-activated Protein Kinase Phosphatase 3, MAP Kinase Phosphatase 3, MKP3, MKP-3, PYST1) (FITC)

Gene Names

DUSP6; HH19; MKP3; PYST1

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti MouseE CD49d: Low Endotoxin)

Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD49d: Alexa Fluor 647)

Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD49d: FITC)

Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD49d)

Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD49d)

Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD49d: Azide Free)

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CD49d, Monoclonal Antibody (Cat# AAA11898)

Full Name

RAT ANTI MOUSE CD49d:Low Endotoxin

Gene Names

Itga4; CD49D; Itga4B

Applications

Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation

Pricing

$915/0.5 mg $1,755/2x0.5 mg $2,550/3x0.5 mg $3,300/4x0.5 mg $3,790/5x0.5 mg

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Application Data (Analysis of Protein Array containing more than 19,000 full-length human proteins using AMACR/p504S Mouse Monoclonal Antibody (AMACR/1864). Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

SDS-PAGE (SDS-PAGE Analysis Purified AMACR/p504S Mouse Monoclonal Antibody (AMACR/1864) Confirmation of Purity and Integrity of Antibody.)

WB (Western Blot) (Western Blot of Human Prostate Cancer PC3 Cell lysate AMACR/p504S Mouse Monoclonal Antibody (AMACR/1864).)

WB (Western Blot) (Western Blot (1) Recombinant AMACR and (2) Human Kidney lysate using AMACR/p504S Mouse Monoclonal Antibody (AMACR/1864).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with AMACR/p504S Mouse Monoclonal Antibody (AMACR/1864).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Prostate Carcinoma stained with AMACR/p504S Mouse Monoclonal Antibody (AMACR/1864).)

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AMACR/p504S, Monoclonal Antibody (Cat# AAA23906)

Full Name

AMACR/p504S (Prostate Cancer Marker)

Gene Names

AMACR; RM; RACE; CBAS4; P504S; AMACRD

Reactivity

Human. Others not known.

Applications

Western Blot, Immunohistochemistry

Purity

Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Pricing

$295/0.02 mg (With BSA & Azide at 0.2mg/mL) $475/0.1 mg (With BSA & Azide at 0.2mg/mL) $475/0.1 mg (Without BSA & Azide at 1mg/mL) $905/2x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,310/3x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,690/4x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,900/5x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,900/5x0.1 mg (Without BSA & Azide at 1mg/mL)

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IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human pancreas. 1, Antibody was diluted at 1:200(4 degree ,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human pancreas. 1, Antibody was diluted at 1:200(4 degree ,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded Human pancreas. 1, Antibody was diluted at 1:200(4 degree ,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:400(4 degree ,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:400(4 degree ,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:400(4 degree ,overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

IF (Immunofluorescence) (Immunofluorescence analysis of Rat-lung tissue. 1,CD4 Monoclonal Antibody(11A1)(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

IF (Immunofluorescence) (Immunofluorescence analysis of Mouse-colon tissue. 1,CD4 Monoclonal Antibody(11A1)(red) was diluted at 1:200(4 degree C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300(room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue. 1,CD4 Monoclonal Antibody(11A1) was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1,CD4 Monoclonal Antibody(11A1) was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human-stomach tissue. 1,CD4 Monoclonal Antibody(11A1) was diluted at 1:200(4 degree C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C,20min). 3,Secondary antibody was diluted at 1:200(room tempeRature, 30min). Negative control was used by secondary antibody only.)

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CD4, Monoclonal Antibody (Cat# AAA23995)

Full Name

CD4 (7H9) Mouse mAb

Gene Names

CD4; CD4mut

Reactivity

Human, Rat, Mouse

Applications

IHC-P

Purity

Affinity purified

Pricing

$565/0.1 mL $1,070/2x0.1 mL $1,545/3x0.1 mL $1,900/5x0.1 mL $1,995/4x0.1 mL

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WB (Western Blot) (SLC25A13 monoclonal antibody Western Blot analysis of SLC25A13 expression in HepG2)

WB (Western Blot) (Western blot analysis of SLC25A13 over-expressed 293 cell line, cotransfected with SLC25A13 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with SLC25A13 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged SLC25A13 is ~0.3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SLC25A13 on HepG2 cell. [antibody concentration 10ug/ml])

WB (Western Blot) (Western Blot analysis of SLC25A13 expression in transfected 293T cell line by SLC25A13 monoclonal antibody Lane 1: SLC25A13 transfected lysate (74.2kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (34.8kD).)

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SLC25A13, Monoclonal Antibody (Cat# AAA25542)

Full Name

SLC25A13 (Calcium-binding Mitochondrial Carrier Protein Aralar2, Mitochondrial Aspartate Glutamate Carrier 2, Solute Carrier Family 25 Member 13, Citrin, ARALAR2) (HRP)

Gene Names

SLC25A13; CTLN2; NICCD; CITRIN; ARALAR2

Reactivity

Human

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IP (Immunoprecipitation) (Dilution: WB: 1:500-2000 IP:1:200 IF 1:200 IHC 1:50-300)

WB (Western Blot) (Dilution: WB: 1:500-2000 IP:1:200 IF 1:200 IHC 1:50-300)

IF (Immunofluorescence) (Dilution: WB: 1:500-2000 IP:1:200 IF 1:200 IHC 1:50-300)

IF (Immunofluorescence) (Dilution: WB: 1:500-2000 IP:1:200 IF 1:200 IHC 1:50-300)

IHC (Immunohistochemistry-Paraffin) (Dilution: WB: 1:500-2000 IP:1:200 IF 1:200 IHC 1:50-300)

IHC (Immunohistochemistry-Paraffin) (Dilution: WB: 1:500-2000 IP:1:200 IF 1:200 IHC 1:50-300)

WB (Western Blot) (Dilution: WB: 1:500-2000 IP:1:200 IF 1:200 IHC 1:50-300)

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Histone H3, Monoclonal Antibody (Cat# AAA28882)

Full Name

Histone H3 (Tri Methyl Lys79) Monoclonal Antibody (3G3)

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, IF, IP

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

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WB (Western Blot) (CCNH monoclonal antibody, Western Blot analysis of CCNH expression in HeLa.)

Application Data (Detection limit for recombinant GST tagged CCNH is ~1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CCNH on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CCNH on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of CCNH expression in transfected 293T cell line by CCNH monoclonal antibody. Lane 1: CCNH transfected lysate (37.6kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (37.73kD).)

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CCNH, Monoclonal Antibody (Cat# AAA25341)

Full Name

CCNH (Cyclin H, Cyclin-H, MO15-associated Protein, p34, p37) (HRP)

Gene Names

CCNH; CAK; p34; p37; CycH

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IHC (Immunohistochemistry) (Figure 8. IHC analysis of Liver Carboxylesterase 1/CES1 using anti-Liver Carboxylesterase 1/CES1 antibody (AAA19363).Liver Carboxylesterase 1/CES1 was detected in paraffin-embedded section of rat liver tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Liver Carboxylesterase 1/CES1 Antibody (AAA19363) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of Liver Carboxylesterase 1/CES1 using anti-Liver Carboxylesterase 1/CES1 antibody (AAA19363).Liver Carboxylesterase 1/CES1 was detected in paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Liver Carboxylesterase 1/CES1 Antibody (AAA19363) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of Liver Carboxylesterase 1/CES1 using anti-Liver Carboxylesterase 1/CES1 antibody (AAA19363).Liver Carboxylesterase 1/CES1 was detected in paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Liver Carboxylesterase 1/CES1 Antibody (AAA19363) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of Liver Carboxylesterase 1/CES1 using anti-Liver Carboxylesterase 1/CES1 antibody (AAA19363).Liver Carboxylesterase 1/CES1 was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Liver Carboxylesterase 1/CES1 Antibody (AAA19363) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of Liver Carboxylesterase 1/CES1 using anti-Liver Carboxylesterase 1/CES1 antibody (AAA19363).Liver Carboxylesterase 1/CES1 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Liver Carboxylesterase 1/CES1 Antibody (AAA19363) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

FCM (Flow Cytometry) (Figure 3. Flow Cytometry analysis of HEPG2 cells using anti- Liver Carboxylesterase 1/CES1 antibody (AAA19363).Overlay histogram showing HEPG2 cells stained with AAA19363 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Liver Carboxylesterase 1/CES1 Antibody (AAA19363, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 2. IF analysis of Liver Carboxylesterase 1/CES1 using anti- Liver Carboxylesterase 1/CES1 antibody (AAA19363).Liver Carboxylesterase 1/CES1 was detected in immunocytochemical section of HEPG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti- Liver Carboxylesterase 1/CES1 Antibody (AAA19363) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

WB (Western Blot) (Figure 1. Western blot analysis of Liver Carboxylesterase 1/CES1 using anti-Liver Carboxylesterase 1/CES1 antibody (AAA19363).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human THP-1 whole cell lysatesLane 2: human HEPG2 whole cell lysatesLane 3: rat liver tissue lysatesLane 4: mouse liver tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti-Liver Carboxylesterase 1/CES1 antigen affinity purified monoclonal antibody (Catalog # AAA19363) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for Liver Carboxylesterase 1/CES1 at approximately 63KD. The expected band size for Liver Carboxylesterase 1/CES1 is at 63KD.)

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Liver Carboxylesterase 1/CES1, Monoclonal Antibody (Cat# AAA19363)

Full Name

Anti-Liver Carboxylesterase 1/CES1 Antibody (monoclonal, 3F10)

Gene Names

CES1; CEH; REH; TGH; ACAT; CE-1; CES2; HMSE; SES1; HMSE1; PCE-1; hCE-1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, ICC, IF, FC/FACS/FCM

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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Application Data (Detection limit for recombinant GST tagged MECP2 is ~1ng/ml as a capture antibody.)

IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to MECP2 on formalin-fixed paraffin-embedded human heart. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of MECP2 expression in transfected 293T cell line by MECP2 monoclonal antibody. Lane 1: MECP2 transfected lysate (52.4kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (MECP2 monoclonal antibody. Western Blot analysis of MECP2 expression in NIH/3T3.)

WB (Western Blot) (MECP2 monoclonal antibody Western Blot analysis of MECP2 expression in MCF-7,)

WB (Western Blot) (MECP2 monoclonal antibody. Western Blot analysis of MECP2 expression in rat muscle.)

WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)

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MECP2, Monoclonal Antibody (Cat# AAA24566)

Full Name

MECP2 (Methyl-CpG-binding Protein 2, MeCp-2 Protein, MeCp2, DKFZp686A24160) APC

Gene Names

MECP2; RS; RTS; RTT; PPMX; MRX16; MRX79; MRXSL; AUTSX3; MRXS13

Reactivity

Human, Mouse, Rat

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (RPS6KB1 monoclonal antibody (M03), clone 2C2. Western Blot analysis of RPS6KB1 expression in PC-12.)

WB (Western Blot) (RPS6KB1 monoclonal antibody (M03), clone 2C2. Western Blot analysis of RPS6KB1 expression in NIH/3T3.)

Application Data (Detection limit for recombinant GST tagged RPS6KB1 is approximately 0.1ng/ml as a capture antibody.)

WB (Western Blot) (RPS6KB1 monoclonal antibody (M03), clone 2C2 Western Blot analysis of RPS6KB1 expression in A-431.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RPS6KB1 on A-431 cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RPS6KB1 on A-431 cell. [antibody concentration 10 ug/ml])

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RPS6KB1, Monoclonal Antibody (Cat# AAA26103)

Full Name

RPS6KB1 (Ribosomal Protein S6 Kinase, 70kD, Polypeptide 1, PS6K, S6K, S6K1, STK14A, p70(S6K)-alpha, p70-S6K, p70-alpha) (APC)

Gene Names

RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha

Applications

IF, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of Daudi cells using anti-HLA-DRA antibody (AAA19681).Overlay histogram showing Daudi cells stained with AAA19681 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-HLA-DRA Antibody (AAA19681, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of HLA-DRA using anti-HLA-DRA antibody (AAA19681).HLA-DRA was detected in a paraffin-embedded section of human bladder epithelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-HLA-DRA Antibody (AAA19681) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of HLA-DRA using anti-HLA-DRA antibody (AAA19681).HLA-DRA was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-HLA-DRA Antibody (AAA19681) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of HLA-DRA using anti-HLA-DRA antibody (AAA19681).HLA-DRA was detected in a paraffin-embedded section of human hepatocellular carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-HLA-DRA Antibody (AAA19681) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of HLA-DRA using anti-HLA-DRA antibody (AAA19681).HLA-DRA was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-HLA-DRA Antibody (AAA19681) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of HLA-DRA using anti-HLA-DRA antibody (AAA19681).HLA-DRA was detected in a paraffin-embedded section of human colonic adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-HLA-DRA Antibody (AAA19681) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of HLA-DRA using anti-HLA-DRA antibody (AAA19681).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Raji whole cell lysates,Lane 2: human Daudi whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HLA-DRA antigen affinity purified monoclonal antibody (#AAA19681) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HLA-DRA at approximately 35-37 kDa. The expected band size for HLA-DRA is at 29 kDa.)

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HLA-DR/HLA-DRA, Monoclonal Antibody (Cat# AAA19681)

Full Name

Anti-HLA-DR/HLA-DRA Antibody Picoband (monoclonal, 5B13F7)

Gene Names

HLA-DRA; MLRW; HLA-DRA1

Reactivity

Human

Applications

WB, IHC, FC/FACS

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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WB (Western Blot) (Western blot analysis of ARID3A over-expressed 293 cell line, cotransfected with ARID3A Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ARID3A monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged ARID3A is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ARID3A on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ARID3A on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml].)

WB (Western Blot) (ARID3A monoclonal antibody, Western Blot analysis of ARID3A expression in K-562.)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

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ARID3A, Monoclonal Antibody (Cat# AAA14788)

Full Name

ARID3A (AT-rich Interactive Domain-containing Protein 3A, ARID Domain-containing Protein 3A, B-cell Regulator of IgH Transcription, Bright, Dead Ringer-like Protein 1, E2F-binding Protein 1, DRIL1, DRIL3, DRX, E2FBP1)

Gene Names

ARID3A; DRIL1; DRIL3; BRIGHT; E2FBP1

Reactivity

Human

Applications

EL/EIA, WB, IHC, IF

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

$820/0.1 mg $1,565/2x0.1 mg $2,270/3x0.1 mg $2,940/4x0.1 mg $3,380/5x0.1 mg

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WB (Western Blot) (DAAM1 monoclonal antibody Western Blot analysis of DAAM1 expression in A-431.)

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between RHOA and DAAM1 HeLa cells were stained with RHOA rabbit purified polyclonal 1:1200 and DAAM1 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Application Data (Detection limit for recombinant GST tagged DAAM1 is ~1ng/ml as a capture antibody.)

WB (Western Blot) (Western Blot analysis of DAAM1 expression in transfected 293T cell line by DAAM1 monoclonal antibody. Lane 1: DAAM1 transfected lysate (122.3kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (DAAM1 monoclonal antibody Western Blot analysis of DAAM1 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (38.21kD).)

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DAAM1, Monoclonal Antibody (Cat# AAA24775)

Full Name

DAAM1 (Disheveled-associated Activator Of Morphogenesis 1, KIAA0666) (Biotin)

Reactivity

Human, Rat

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IP (Immunoprecipitation) (Immunoprecipitation of SOX9 transfected lysate using SOX9 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with SOX9 rabbit polyclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SOX9 on HepG2 cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SOX9 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 0.7ug/ml].)

WB (Western Blot) (Western Blot analysis of SOX9 expression in transfected 293T cell line by SOX9 monoclonal antibody. Lane 1: SOX9 transfected lysate (56.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (SOX9 monoclonal antibody, Western Blot analysis of SOX9 expression in HepG2.)

WB (Western Blot) (Western Blot detection against Immunogen (38.21kD).)

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SOX9, Monoclonal Antibody (Cat# AAA25554)

Full Name

SOX9 (Transcription Factor SOX-9, SOX 9) (HRP)

Gene Names

SOX9; CMD1; SRA1; CMPD1

Reactivity

Human

Applications

EIA, IHC, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (MGC21874 monoclonal antibody. Western Blot analysis of MGC21874 expression in PC-12.)

WB (Western Blot) (MGC21874 monoclonal antibody, Western Blot analysis of MGC21874 expression in HeLa.)

Application Data (Detection limit for recombinant GST tagged MGC21874 is ~0.3ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to MGC21874 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1.5ug/ml].)

WB (Western Blot) (MGC21874 monoclonal antibody. Western Blot analysis of MGC21874 expression in NIH/3T3.)

WB (Western Blot) (MGC21874 monoclonal antibody. Western Blot analysis of MGC21874 expression in Raw 264.7.)

WB (Western Blot) (Western Blot detection against Immunogen (38.1kD).)

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TADA2B, Monoclonal Antibody (Cat# AAA24051)

Full Name

TADA2B (Transcriptional Adapter 2-beta, ADA2-like Protein beta, ADA2-beta, ADA2B, MGC21874)

Gene Names

tada2b; wu:fb72h11; wu:fi12b07; wu:fi33a06; zgc:110397

Reactivity

Human, Mouse, Rat

Applications

EL/EIA, WB, IHC

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

$820/0.1 mg $1,565/2x0.1 mg $2,270/3x0.1 mg $2,940/4x0.1 mg $3,380/5x0.1 mg

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Application Data (Detection limit for recombinant GST tagged SNRPA is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SNRPA on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SNRPA on formalin-fixed paraffin-embedded human heart tissue. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of SNRPA expression in transfected 293T cell line by SNRPA monoclonal antibody. Lane 1: SNRPA transfected lysate (31.3kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (SNRPA monoclonal antibody, Western Blot analysis of SNRPA expression in Hela.)

WB (Western Blot) (Western Blot detection against Immunogen (57.13kD).)

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SNRPA, Monoclonal Antibody (Cat# AAA25847)

Full Name

SNRPA (U1 Small Nuclear Ribonucleoprotein A, U1 snRNP A, U1-A, U1A) (PE)

Gene Names

SNRPA; U1A; Mud1; U1-A

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IHC (Immunohistochemistry) (Figure 11. IHC analysis of U2AF65/U2AF2 using anti U2AF65/U2AF2 antibody (AAA19376).U2AF65/U2AF2 was detected in paraffin-embedded section of human gallbladder adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-U2AF65/U2AF2 Antibody (AAA19376) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of A549 cells using anti- U2AF65/U2AF2 antibody (AAA19376).Overlay histogram showing A549 cells stained with AAA19376 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-U2AF65/U2AF2 Antibody (AAA19376, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 9. IF analysis of U2AF65/U2AF2 using anti- U2AF65/U2AF2 antibody (AAA19376).U2AF65/U2AF2 was detected in immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti- U2AF65/U2AF2 Antibody (AAA19376) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 8. IHC analysis of U2AF65/U2AF2 using anti U2AF65/U2AF2 antibody (AAA19376).U2AF65/U2AF2 was detected in paraffin-embedded section of rat intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-U2AF65/U2AF2 Antibody (AAA19376) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of U2AF65/U2AF2 using anti U2AF65/U2AF2 antibody (AAA19376).U2AF65/U2AF2 was detected in paraffin-embedded section of mouse intestine tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-U2AF65/U2AF2 Antibody (AAA19376) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of U2AF65/U2AF2 using anti U2AF65/U2AF2 antibody (AAA19376).U2AF65/U2AF2 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-U2AF65/U2AF2 Antibody (AAA19376) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of U2AF65/U2AF2 using anti U2AF65/U2AF2 antibody (AAA19376).U2AF65/U2AF2 was detected in paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-U2AF65/U2AF2 Antibody (AAA19376) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of U2AF65/U2AF2 using anti U2AF65/U2AF2 antibody (AAA19376).U2AF65/U2AF2 was detected in paraffin-embedded section of human colonic adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-U2AF65/U2AF2 Antibody (AAA19376) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of U2AF65/U2AF2 using anti U2AF65/U2AF2 antibody (AAA19376).U2AF65/U2AF2 was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-U2AF65/U2AF2 Antibody (AAA19376) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of U2AF65/U2AF2 using anti U2AF65/U2AF2 antibody (AAA19376).U2AF65/U2AF2 was detected in paraffin-embedded section of human gallbladder adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-U2AF65/U2AF2 Antibody (AAA19376) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of U2AF65/U2AF2 using anti-U2AF65/U2AF2 antibody (AAA19376).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HEK293 whole cell lysatesLane 2: human THP-1 whole cell lysatesLane 3: human U20S whole cell lysatesLane 4: human Jurkat whole cell lysatesLane 5: rat PC-12 whole cell lysatesLane 6: mouse brain tissue lysatesLane 7: rat brain tissue lysatesLane 8: mouse RAW264. 7 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti-U2AF65/U2AF2 antigen affinity purified monoclonal antibody (Catalog # AAA19376) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for U2AF65/U2AF2 at approximately 65KD. The expected band size for U2AF65/U2AF2 is at 65KD.)

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U2AF65/U2AF2, Monoclonal Antibody (Cat# AAA19376)

Full Name

Anti-U2AF65/U2AF2 Antibody (monoclonal, 3G9)

Gene Names

U2AF2; U2AF65

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, ICC, IF, FC/FACS/FCM

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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WB (Western Blot) (EGFR Antibody (C-term) western blot analysis in Hela cell line lysates (35ug/lane).This demonstrates the EGFR antibody detected the EGFR protein (arrow).)

WB (Western Blot) (Western blot analysis of lysates from A431, MCF-7 cell line (from left to right), using EGFR Antibody (C-term). AAA28637 was diluted at 1:1000 at each lane. A goat anti-mouse IgG H&L(HRP) at 1:3000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded R. esophagus section using EGFR Antibody (C-term). AAA28637 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded M. esophagus section using EGFR Antibody (C-term). AAA28637 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded H. stomach section using EGFR Antibody (C-term). AAA28637 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded H. esophagus section using EGFR Antibody (C-term). AAA28637 was diluted at 1:25 dilution. A peroxidase-conjugated goat anti-rabbit IgG at 1:400 dilution was used as the secondary antibody, followed by DAB staining.)

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EGFR, Monoclonal Antibody (Cat# AAA28637)

Full Name

EGFR Antibody (C-term)

Gene Names

EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2

Reactivity

Human

Applications

WB, EIA, IHC

Pricing

$275/0.08 mL $510/0.4 mL $975/2x0.4 mL $1,415/3x0.4 mL $1,825/4x0.4 mL $2,070/5x0.4 mL

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Application Data (Detection limit for recombinant GST tagged PHB is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PHB on HeLa cell. [antibody concentration 1 ~ 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PHB on formalin-fixed paraffin-embedded human tonsil tissue.[antibody concentration 5ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PHB on formalin-fixed paraffin-embedded human colon adenocarcinoma. [antibody concentration 5ug/ml])

WB (Western Blot) (PHB monoclonal antibody. Western Blot analysis of PHB expression in Hela.)

WB (Western Blot) (Western Blot detection against Immunogen (55.66kD).)

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PHB, Monoclonal Antibody (Cat# AAA24313)

Full Name

PHB (Prohibitin) (AP)

Gene Names

PHB; PHB1; HEL-215; HEL-S-54e

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western blot analysis of PGM1 over-expressed 293 cell line, cotransfected with PGM1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PGM1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged PGM1 is 0.3ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of PGM1 transfected lysate using PGM1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PGM1 rabbit polyclonal antibody)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PGM1 on HeLa cell. [antibody concentration 20ug/ml])

WB (Western Blot) (Western Blot analysis of PGM1 expression in transfected 293T cell line by PGM1 monoclonal antibody. Lane 1: PGM1 transfected lysate (61.4kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (PGM1 monoclonal antibody Western Blot analysis of PGM1 expression in Jurkat.)

WB (Western Blot) (Western Blot detection against Immunogen (87.56kD).)

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PGM1, Monoclonal Antibody (Cat# AAA24310)

Full Name

PGM1 (Phosphoglucomutase-1, Glucose Phosphomutase 1) (AP)

Gene Names

PGM1; CDG1T; GSD14

Reactivity

Human

Applications

EIA, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western blot analysis of PIP5K3 over-expressed 293 cell line, cotransfected with PIP5K3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PIP5K3 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged PIP5K3 is ~0.3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PIP5K3 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PIP5K3 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of PIP5K3 expression in transfected 293T cell line by PIP5K3 monoclonal antibody. Lane 1: PIP5K3 transfected lysate (50.2kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (38.21kD).)

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PIP5K3, Monoclonal Antibody (Cat# AAA24317)

Full Name

PIP5K3 (PIKFYVE, 1-phosphatidylinositol 3-phosphate 5-kinase, Phosphatidylinositol 3-phosphate 5-kinase, FYVE Finger-containing Phosphoinositide Kinase, PIKfyve, Phosphatidylinositol 3-phosphate 5-kinase Type III, PIPkin-III, Type III PIP Kinase, KIAA0981

Gene Names

PIKFYVE; CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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ICC (Immunocytochemistry) (ICC staining STAT5b in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining STAT5b in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining STAT5b in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-STAT5b antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-STAT5b antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-STAT5b antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-STAT5b antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of STAT5b on different lysates using anti-STAT5b antibody at 1/1, 000 dilution. Positive control: Lane 1: K562 Lane 2: Hela)

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STAT5b, Monoclonal Antibody (Cat# AAA30207)

Full Name

STAT5b Antibody

Gene Names

STAT5B; STAT5

Reactivity

Human, Mouse, Rat

Applications

WB, ICC, IF, IHC, IP

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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WB (Western Blot) (DAAM1 monoclonal antibody Western Blot analysis of DAAM1 expression in A-431.)

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between RHOA and DAAM1 HeLa cells were stained with RHOA rabbit purified polyclonal 1:1200 and DAAM1 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Application Data (Detection limit for recombinant GST tagged DAAM1 is ~1ng/ml as a capture antibody.)

WB (Western Blot) (Western Blot analysis of DAAM1 expression in transfected 293T cell line by DAAM1 monoclonal antibody. Lane 1: DAAM1 transfected lysate (122.3kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (DAAM1 monoclonal antibody Western Blot analysis of DAAM1 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (38.21kD).)

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DAAM1, Monoclonal Antibody (Cat# AAA24480)

Full Name

DAAM1 (Disheveled-associated Activator Of Morphogenesis 1, KIAA0666) APC

Reactivity

Human, Rat

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

What are Monoclonal Antibodies?

Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.

This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.

AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.

What Are The Uses of Monoclonal Antibodies

Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.

ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.

Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.

Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.

Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.

How Monoclonal Antibodies Are Used as Medicine?

Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).

Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:

• Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.


• Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.


• Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.

Why Buy Monoclonal Antibodies From Us?

At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.

1. Validated for Versatile Applications

The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).

2. Wide Selection & Specialized Options

We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.

3. High-Quality Proteins

Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.

Frequently Asked Questions

1. Are your monoclonal antibodies validated for specific applications?

Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.

2. How do I choose the right monoclonal antibody for my application?

Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.

3. How quickly can I receive my order?

Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.