Monoclonal Antibodies

Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.

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WB (Western Blot) (CFL1 monoclonal antibody. Western Blot analysis of CFL1 expression in PC-12.)

WB (Western Blot) (CFL1 monoclonal antibody. Western Blot analysis of CFL1 expression in Raw 264.7.)

Application Data (Detection limit for recombinant GST tagged CFL1 is ~10ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CFL1 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CFL1 on formalin-fixed paraffin-embedded human breast cancer. [antibody concentration 1.5ug/ml].)

WB (Western Blot) (CFL1 monoclonal antibody. Western Blot analysis of CFL1 expression in NIH/3T3.)

WB (Western Blot) (Western Blot detection against Immunogen (44kD).)

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CFL1, Monoclonal Antibody (Cat# AAA25645)

Full Name

CFL1 (Cofilin-1, Cofilin, Non-muscle Isoform, 18kD Phosphoprotein, p18, CFL) (PE)

Gene Names

CFL1; CFL; cofilin; HEL-S-15

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (EXOC4 monoclonal antibody. Western Blot analysis of EXOC4 expression in Raw 264.7.)

Application Data (Detection limit for recombinant GST tagged EXOC4 is ~0.1ng/ml as a capture antibody.)

WB (Western Blot) (EXOC4 monoclonal antibody. Western Blot analysis of EXOC4 expression in NIH/3T3.)

WB (Western Blot) (EXOC4 monoclonal antibody, Western Blot analysis of EXOC4 expression in Hela NE.)

WB (Western Blot) (EXOC4 monoclonal antibody. Western Blot analysis of EXOC4 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (38.1kD).)

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EXOC4, Monoclonal Antibody (Cat# AAA25681)

Full Name

EXOC4 (Exocyst Complex Component 4, Exocyst Complex Component Sec8, KIAA1699, SEC8, SEC8L1, MGC27170) (PE)

Gene Names

EXOC4; SEC8; Sec8p; SEC8L1

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western Blot detection against Immunogen (37.44kD).)

WB (Western Blot) (CCT7 monoclonal antibody Western Blot analysis of CCT7 expression in human pancreas.)

Application Data (Detection limit for recombinant GST tagged CCT7 is ~0.3ng/ml as a capture antibody.)

WB (Western Blot) (Western Blot analysis of CCT7 expression in transfected 293T cell line by CCT7 monoclonal antibody. Lane 1: CCT7 transfected lysate (59.4kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (CCT7 monoclonal antibody Western Blot analysis of CCT7 expression in K-562.)

WB (Western Blot) (CCT7 monoclonal antibody Western Blot analysis of CCT7 expression in Raw 264.7)

WB (Western Blot) (CCT7 monoclonal antibody Western Blot analysis of CCT7 expression in HL-60)

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CCT7, Monoclonal Antibody (Cat# AAA24749)

Full Name

CCT7 (T-complex Protein 1 Subunit eta, TCP-1-eta, CCT-eta, HIV-1 Nef-interacting Protein, CCTH, NIP7-1) (Biotin)

Gene Names

CCT7; CCTH; CCTETA; NIP7-1; TCP1ETA

Reactivity

Human, Mouse, Rat

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western blot analysis of CAMK4 over-expressed 293 cell line, cotransfected with CAMK4 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CAMK4 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CAMK4 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CAMK4 on formalin-fixed paraffin-embedded human tonsil tissue. [antibody concentration 5ug/ml].)

WB (Western Blot) (Western Blot analysis of CAMK4 expression in transfected 293T cell line by CAMK4 monoclonal antibody. Lane 1: CAMK4 transfected lysate (52.14kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (CAMK4 monoclonal antibody Western Blot analysis of CAMK4 expression in Jurkat.)

WB (Western Blot) (Western Blot detection against Immunogen (77.66kD).)

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CAMKIV, Monoclonal Antibody (Cat# AAA24743)

Full Name

CAMKIV (Calcium/Calmodulin-dependent Protein Kinase Type IV, CAM Kinase-GR, CaMK IV, CAMK4) (Biotin)

Gene Names

CAMK4; caMK; CaMKIV; CaMK IV; CaMK-GR

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Dilution: WB: 1:1000-5000 IP:1:200 IF 1:200 IHC 1:50-300)

IF (Immunofluorescence) (Dilution: WB: 1:1000-5000 IP:1:200 IF 1:200 IHC 1:50-300)

IF (Immunofluorescence) (Dilution: WB: 1:1000-5000 IP:1:200 IF 1:200 IHC 1:50-300)

IF (Immunofluorescence) (Dilution: WB: 1:1000-5000 IP:1:200 IF 1:200 IHC 1:50-300)

IHC (Immunohistochemistry-Paraffin) (Dilution: WB: 1:1000-5000 IP:1:200 IF 1:200 IHC 1:50-300)

IHC (Immunohistochemistry-Paraffin) (Dilution: WB: 1:1000-5000 IP:1:200 IF 1:200 IHC 1:50-300)

WB (Western Blot) (Dilution: WB: 1:1000-5000 IP:1:200 IF 1:200 IHC 1:50-300)

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Caspase 9, Monoclonal Antibody (Cat# AAA28877)

Full Name

Caspase 9 Monoclonal Antibody (3-20)

Gene Names

CASP9; MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6

Reactivity

Human, Mouse, Rat, Chicken

Applications

WB, IHC-P, IF, IP

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

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IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to ABL2 on formalin-fixed paraffin-embedded human cerebellum. [antibody concentration 1.5 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ABL2 on formalin-fixed paraffin-embedded human cerebellum. [antibody concentration 1.5 ug/ml])

Application Data (Detection limit for recombinant GST tagged ABL2 is approximately 0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ABL2 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ABL2 on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (ABL2 monoclonal antibody (M09), clone 5C6 Western Blot analysis of ABL2 expression in K-562.)

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ABL2, Monoclonal Antibody (Cat# AAA26081)

Full Name

ABL2 (v-abl Abelson murine Leukemia Viral OncoGene Homolog 2 (arg, Abelson-Related Gene), ABLL, ARG, FLJ22224, FLJ31718, FLJ41441) (APC)

Gene Names

ABL2; ARG; ABLL

Applications

IF, IHC, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Detection of human Estrogen Receptor alpha by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, MCF-7, Hep-G2, and MOLT-4 cells prepared using NETN lysis buffer. Antibody: Mouse anti-Estrogen Receptor alpha monoclonal antibody [119-13] (AAA23783 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)

IP (Immunoprecipitation) (Detection of human Estrogen Receptor alpha by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MCF7 cells prepared using NETN lysis buffer. Antibodies: Mouse anti-Estrogen Receptor alpha monoclonal antibody [119-13] (AAA23783 lot 1) used for IP at 6 ug/mg lysate. Estrogen Receptor alpha was also immunoprecipitated by another anti-Estrogen Receptor alpha antibody against a differnet epitope . For blotting immunoprecipitated Estrogen Receptor alpha, A500-026 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.)

IHC (Immunohistochemistry) (Detection of human Estrogen Receptor Alpha in FFPE breast carcinoma by immunohistochemistry. Antibody: Mouse anti-Estrogen Receptor Alpha monoclonal antibody [119-13] (AAA23783 lot 1). Secondary: HRP-conjugated goat anti-mouse IgG . Substrate: DAB.)

IHC (Immunohistochemistry) (Detection of human Estrogen Receptor Alpha in FFPE breast carcinoma by immunohistochemistry. Antibody: Mouse anti-Estrogen Receptor Alpha monoclonal antibody [119-13] (AAA23783 lot 1). Secondary: HRP-conjugated goat anti-mouse IgG . Substrate: DAB.)

ICC (Immunocytochemistry) (Detection of human Estrogen Receptor Alpha in FFPE MCF7 cells by immunocytochemistry. Antibody: Mouse anti-Estrogen Receptor Alpha monoclonal antibody [119-13] (AAA23783 lot 1). Secondary: HRP-conjugated goat anti-mouse IgG . Substrate: DAB.)

FCM (Flow Cytometry) (Detection of human Estrogen Receptor Alpha (shaded) in MCF7 cells by flow cytometry. Antibody: Mouse anti-Estrogen Receptor Alpha monoclonal antibody [119-13] (AAA23783) or secondary antibody only (unshaded). Secondary: DyLight 650-conjugated goat anti-mouse IgG .)

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Estrogen Receptor Alpha, Monoclonal Antibody (Cat# AAA23783)

Full Name

Mouse anti-Estrogen Receptor Alpha Monoclonal Antibody [119-13]

Gene Names

ESR1; ER; ESR; Era; ESRA; NR3A1

Reactivity

Human

Applications

WB, IP, IHC, ICC, FC/FACS

Purity

Purified

Pricing

$295/0.01 mL $680/0.1 mL $980/5x0.01 mL $1,300/2x0.1 mL $1,890/3x0.1 mL $2,440/4x0.1 mL $2,755/5x0.1 mL

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WB (Western Blot) (Detection of mouse KAP-1 by western blot. Samples: Whole cell lysate (50 ug) from TCMK-1 and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-KAP-1 recombinant monoclonal antibody [BL-248-2G6] (AAA23792 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 1 second. Lower Panel: Rabbit anti-Cytoskeletal Actin recombinant monoclonal antibody .)

WB (Western Blot) (Detection of human KAP-1 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, U2OS, HEK293T, Hep-G2, K-562, RKO, and A-549 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-KAP-1 recombinant monoclonal antibody [BL-248-2G6] (AAA23792 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 1 second. Lower Panel: Rabbit anti-Cytoskeletal Actin recombinant monoclonal antibody .)

IP (Immunoprecipitation) (Detection of human KAP-1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Rabbit anti-KAP-1 recombinant monoclonal antibody [BL-248-2G6] (AAA23792 lot 2) used for IP at 20 ul per reaction. KAP-1 was also immunoprecipitated by a previous lot of this antibody (lot AAA23792-1) and rabbit anti-KAP-1 antibodies . For blotting immunoprecipitated KAP-1, AAA23792 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.)

IHC (Immunohistochemistry) (Detection of mouse KAP-1 in FFPE colon carcinoma CT26 by IHC. Antibody: Rabbit anti-KAP-1 recombinant monoclonal antibody [BL-248-2G6] (AAA23792 lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

IHC (Immunohistochemistry) (Detection of human KAP-1 in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-KAP-1 recombinant monoclonal antibody [BL-248-2G6] (AAA23792 lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry) (Detection of human KAP-1 in FFPE SK-MEL-28 cells by ICC. Antibody: Rabbit anti-KAP-1 recombinant monoclonal antibody [BL-248-2G6] (AAA23792 lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

FCM (Flow Cytometry) (Detection of human KAP-1 by flow cytometry. Sample: Jurkat cells were fixed in 4% formaldehyde and permeabilized with 90% methanol. Antibody: 1E6 cells were incubated with 1 ul of rabbit anti-KAP-1 recombinant monoclonal antibody [BL-248-2G6] (AAA23792) (red shaded) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

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KAP-1, Monoclonal Recombinant Antibody (Cat# AAA23792)

Full Name

Rabbit anti-KAP-1 Recombinant Monoclonal Antibody [BL-248-2G6]

Gene Names

TRIM28; KAP1; TF1B; RNF96; TIF1B; PPP1R157

Reactivity

Human, Mouse

Applications

WB, IP, IHC, ICC, FC/FACS

Purity

Purified

Pricing

$295/0.02 mL $480/0.1 mL $915/2x0.1 mL $980/5x0.02 mL $1,325/3x0.1 mL $1,705/4x0.1 mL $1,855/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of SH-SY-5Y cells with Niemann Pick C1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining Niemann Pick C1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Niemann Pick C1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Niemann Pick C1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Niemann Pick C1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Niemann Pick C1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Niemann Pick C1 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Niemann Pick C1 on SiHa cell lysate using anti-Niemann Pick C1 antibody at 1/500 dilution.)

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Niemann Pick C1, Monoclonal Antibody (Cat# AAA30458)

Full Name

Niemann Pick C1 Antibody

Gene Names

NPC1; NPC

Reactivity

Human, Mouse, Rat

Applications

WB, ICC, IF, IHC, FC/FACS

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged HNRPM is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HNRPM on HepG2 cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HNRPM on formalin-fixed paraffin-embedded human thyroid nodular goiter. [antibody concentration 3ug/ml.)

WB (Western Blot) (HNRPM monoclonal antibody. Western Blot analysis of HNRPM expression in NIH/3T3.)

WB (Western Blot) (HNRPM monoclonal antibody Western Blot analysis of HNRPM expression in HepG2.)

WB (Western Blot) (HNRPM monoclonal antibody Western Blot analysis of HNRPM expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (36.3kD).)

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HNRNPM, Monoclonal Antibody (Cat# AAA24830)

Full Name

HNRNPM (Heterogeneous Nuclear Ribonucleoprotein M, hnRNP M, HNRPM, NAGR1, DKFZp547H118) (Biotin)

Gene Names

HNRNPM; CEAR; HNRPM; HTGR1; NAGR1; HNRPM4; HNRNPM4; hnRNP M

Reactivity

Human, Mouse, Rat

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of PC-3 cells using anti-Beclin 1 antibody (AAA19664).Overlay histogram showing PC-3 cells stained with AAA19664 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Beclin 1 Antibody (AAA19664, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 6. IF analysis of Beclin 1 using anti-Beclin 1 antibody (AAA19664).Beclin 1 was detected in an immunocytochemical section of HepG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL mouse anti-Beclin 1 Antibody (AAA19664) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of Beclin 1 using anti-Beclin 1 antibody (AAA19664).Beclin 1 was detected in a paraffin-embedded section of human colonic adenoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Beclin 1 Antibody (AAA19664) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of Beclin 1 using anti-Beclin 1 antibody (AAA19664).Beclin 1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Beclin 1 Antibody (AAA19664) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of Beclin 1 using anti-Beclin 1 antibody (AAA19664).Beclin 1 was detected in a paraffin-embedded section of human hepatocellular carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Beclin 1 Antibody (AAA19664) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of Beclin 1 using anti-Beclin 1 antibody (AAA19664).Beclin 1 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Beclin 1 Antibody (AAA19664) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of Beclin 1 using anti-Beclin 1 antibody (AAA19664).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human 293T whole cell lysates,Lane 2: human Hela whole cell lysates,Lane 3: rat kidney tissue lysates,Lane 4: mouse kidney tissue lysates,Lane 5: mouse NIH/3T3 whole cell lysates,Lane 6: mouse RAW264.7 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Beclin 1 antigen affinity purified monoclonal antibody (#AAA19664) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Beclin 1 at approximately 52-60 kDa. The expected band size for Beclin 1 is at 52 kDa.)

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Beclin 1, Monoclonal Antibody (Cat# AAA19664)

Full Name

Anti-Beclin 1 Antibody Picoband (monoclonal, 2D12A3)

Gene Names

BECN1; ATG6; VPS30; beclin1

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ICC, IF, FC/FACS

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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Application Data (Analysis of Protein Array containing more than 19, 000 full-length human proteins using Mouse Emerin Monoclonal Antibody (EMD/2167) Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

SDS-PAGE (SDS-PAGE Analysis Purified Emerin Mouse Monoclonal Antibody (EMD/2167).Confirmation of Integrity and Purity of Antibody.)

WB (Western Blot) (Western Blot Analysis of human HeLa Cell lysate using Emerin Mouse Monoclonal Antibody (EMD/2167).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Renal Cell Carcinoma stained with Emerin Mouse Monoclonal Antibody (EMD/2167).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Basal Cell Carcinoma stained with Emerin Mouse Monoclonal Antibody (EMD/2167).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with Emerin Mouse Monoclonal Antibody (EMD/2167).)

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Emerin, Monoclonal Antibody (Cat# AAA23898)

Full Name

Emerin (Papillary Thyroid Carcinoma and EDMD Marker)

Gene Names

EMD; STA; EDMD; LEMD5

Reactivity

Human. Others not known.

Applications

Immunohistochemistry

Pricing

$295/0.02 mg (With BSA & Azide at 0.2mg/mL) $475/0.1 mg (With BSA & Azide at 0.2mg/mL) $475/0.1 mg (Without BSA & Azide at 1mg/mL) $905/2x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,310/3x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,690/4x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,900/5x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,900/5x0.1 mg (Without BSA & Azide at 1mg/mL)

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WB (Western Blot) (DNAJC10 monoclonal antibody. Western Blot analysis of DNAJC10 expression in HeLa.)

WB (Western Blot) (DNAJC10 monoclonal antibody. Western Blot analysis of DNAJC10 expression in Raw 264.7.)

Application Data (Detection limit for recombinant GST tagged DNAJC10 is ~10ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to DNAJC10 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to DNAJC10 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3ug/ml].)

WB (Western Blot) (DNAJC10 monoclonal antibody. Western Blot analysis of DNAJC10 expression in NIH/3T3.)

WB (Western Blot) (Western Blot detection against Immunogen (37.77kD).)

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DNAJC10, Monoclonal Antibody (Cat# AAA25670)

Full Name

DNAJC10 (DnaJ Homolog Subfamily C Member 10, ER-resident Protein ERdj5, Macrothioredoxin, MTHr, ERDJ5, UNQ495/PRO1012, DKFZp434J1813, MGC104194) (PE)

Gene Names

DNAJC10; JPDI; MTHr; ERdj5; PDIA19

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged PGR is approximately 0.1ng/ml as a capture antibody.)

WB (Western Blot) (PGR monoclonal antibody (M04), clone 2C2 Western Blot analysis of PGR expression in A-431.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PGR on A-431 cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PGR on A-431 cell. [antibody concentration 10 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PGR on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 1.5 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to PGR on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 1.5 ug/ml])

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PGR, Monoclonal Antibody (Cat# AAA26058)

Full Name

PGR (Progesterone Receptor, NR3C3, PR) (APC)

Gene Names

PGR; PR; NR3C3

Applications

IF, IHC, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (RAD18 monoclonal antibody, Western Blot analysis of RAD18 expression in Hela NE.)

WB (Western Blot) (Western blot analysis of RAD18 over-expressed 293 cell line, cotransfected with RAD18 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with RAD18 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged RAD18 is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RAD18 on HeLa cell. [antibody concentration 25ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RAD18 on formalin-fixed paraffin-embedded human testis. [antibody concentration 1.5ug/ml].)

WB (Western Blot) (Western Blot analysis of RAD18 expression in transfected 293T cell line by RAD18 monoclonal antibody. Lane 1: RAD18 transfected lysate (56.2kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (37kD).)

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RAD18, Monoclonal Antibody (Cat# AAA25812)

Full Name

RAD18 (E3 Ubiquitin-protein Ligase RAD18, Postreplication Repair Protein RAD18, hRAD18, hHR18, RING Finger Protein 73, RNF73) (PE)

Gene Names

RAD18; RNF73

Reactivity

Human, Rat

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (CFL1 monoclonal antibody. Western Blot analysis of CFL1 expression in PC-12.)

WB (Western Blot) (CFL1 monoclonal antibody. Western Blot analysis of CFL1 expression in Raw 264.7.)

Application Data (Detection limit for recombinant GST tagged CFL1 is ~10ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CFL1 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CFL1 on formalin-fixed paraffin-embedded human breast cancer. [antibody concentration 1.5ug/ml].)

WB (Western Blot) (CFL1 monoclonal antibody. Western Blot analysis of CFL1 expression in NIH/3T3.)

WB (Western Blot) (Western Blot detection against Immunogen (44kD).)

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CFL1, Monoclonal Antibody (Cat# AAA25056)

Full Name

CFL1 (Cofilin-1, Cofilin, Non-muscle Isoform, 18kD Phosphoprotein, p18, CFL) (FITC)

Gene Names

CFL1; CFL; cofilin; HEL-S-15

Reactivity

Human, Mouse

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of SiHa cells using anti- Aldolase/ALDOA antibody (AAA19385).Overlay histogram showing SiHa cells stained with AAA19385 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Aldolase/ALDOA Antibody (AAA19385, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of Aldolase/ALDOA using anti- Aldolase/ALDOA antibody (AAA19385).Aldolase/ALDOA was detected in immunocytochemical section of HEPG2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti- Aldolase/ALDOA Antibody (AAA19385) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of Aldolase/ALDOA using anti-Aldolase/ALDOA antibody (AAA19385).Aldolase/ALDOA was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Aldolase/ALDOA Antibody (AAA19385) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of Aldolase/ALDOA using anti-Aldolase/ALDOA antibody (AAA19385).Aldolase/ALDOA was detected in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Aldolase/ALDOA Antibody (AAA19385) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of Aldolase/ALDOA using anti-Aldolase/ALDOA antibody (AAA19385).Aldolase/ALDOA was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Aldolase/ALDOA Antibody (AAA19385) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of Aldolase/ALDOA using anti-Aldolase/ALDOA antibody (AAA19385).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HEPG2 whole cell lysatesLane 2: human A549 whole cell lysatesLane 3: human PC-3whole cell lysatesLane 4: human Hek293 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti-Aldolase/ALDOA antigen affinity purified monoclonal antibody (Catalog # AAA19385) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for Aldolase/ALDOA at approximately 39KD. The expected band size for Aldolase/ALDOA is at 39KD.)

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Aldolase/ALDOA, Monoclonal Antibody (Cat# AAA19385)

Full Name

Anti-Aldolase/ALDOA Antibody (monoclonal, 6H8)

Gene Names

ALDOA; ALDA; GSD12

Reactivity

Human

Applications

WB, IHC-P, ICC, IF, FC/FACS/FCM

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD43 antibody followed by peroxidase conjugated Goat anti Mouse IgG1 antibody for detection. Med power)

Application Data (Immunofluoresce stainng of rat lymph node cryosection with Mouse anti Rat CD43 antibody in red and Mouse anti Rat CD4 in green. Merged image is on the right. Medium power)

Application Data (Immunofluoresce stainng of rat lymph node cryosection with Mouse anti Rat CD43 antibody in red and Mouse anti Rat CD4 in green. Merged image is on the right. Low power)

Application Data (Immunofluoresce stainng of rat lymph node cryosection with Mouse anti Rat CD43 antibody in red and Mouse anti Rat CD4 in green. Merged image is on the right. High power)

Application Data (Staining of rat splenocytes with Mouse anti Rat CD43: FITC)

Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD43 antibody followed by peroxidase conjugated Goat anti Mouse IgG1 antibody for detection. High power)

Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD43 antibody followed by peroxidase conjugated Goat anti Mouse IgG1 antibody for detection. Low power)

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CD43, Monoclonal Antibody (Cat# AAA11989)

Full Name

MOUSE ANTI RAT CD43

Gene Names

Spn; Lsn; Cd43; Lsn1; gpL115

Applications

Immunohistochemistry, Flow Cytometry, Immunohistochemistry

Pricing

$460/0.1 mg $875/2x0.1 mg $1,260/3x0.1 mg $1,635/4x0.1 mg $1,760/5x0.1 mg

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Application Data (Detection limit for recombinant GST tagged SRPK1 is ~0.03ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SRPK1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1ug/ml].)

WB (Western Blot) (Western Blot analysis of SRPK1 expression in transfected 293T cell line by SRPK1 monoclonal antibody. Lane 1: SRPK1 transfected lysate (74.3kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (SRPK1 monoclonal antibody, Western Blot analysis of SRPK1 expression in IMR-32.)

WB (Western Blot) (SRPK1 monoclonal antibody. Western Blot analysis of SRPK1 expression in HepG2.)

WB (Western Blot) (Western Blot detection against Immunogen (37kD).)

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SRPK1, Monoclonal Antibody (Cat# AAA24375)

Full Name

SRPK1 (SRSF Protein Kinase 1, SFRS Protein Kinase 1, Serine/Arginine-rich Protein-specific Kinase 1, SR-protein-specific Kinase 1) (AP)

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western blot analysis of PIP5K3 over-expressed 293 cell line, cotransfected with PIP5K3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PIP5K3 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged PIP5K3 is ~0.3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PIP5K3 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PIP5K3 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of PIP5K3 expression in transfected 293T cell line by PIP5K3 monoclonal antibody. Lane 1: PIP5K3 transfected lysate (50.2kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (38.21kD).)

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PIP5K3, Monoclonal Antibody (Cat# AAA24613)

Full Name

PIP5K3 (PIKFYVE, 1-phosphatidylinositol 3-phosphate 5-kinase, Phosphatidylinositol 3-phosphate 5-kinase, FYVE Finger-containing Phosphoinositide Kinase, PIKfyve, Phosphatidylinositol 3-phosphate 5-kinase Type III, PIPkin-III, Type III PIP Kinase, KIAA0981

Gene Names

PIKFYVE; CFD; FAB1; HEL37; PIP5K; PIP5K3; ZFYVE29

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western Blot detection against Immunogen (70kD).)

WB (Western Blot) (Western blot analysis of LEF1 over-expressed 293 cell line, cotransfected with LEF1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with LEF1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged LEF1 is ~0.3ng/ml as a capture antibody.)

WB (Western Blot) (Western Blot analysis of LEF1 expression in transfected 293T cell line by LEF1 monoclonal antibody. Lane 1: LEF1 transfected lysate (44.2kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (LEF1 monoclonal antibody, Western Blot analysis of LEF1 expression in MES-SA/Dx5.)

WB (Western Blot) (LEF1 monoclonal antibody. Western Blot analysis of LEF1 expression in HL-60.)

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LEF1, Monoclonal Antibody (Cat# AAA24256)

Full Name

LEF1 (Lymphoid Enhancer-binding Factor 1, T Cell-specific Transcription Factor 1-alpha, DKFZp586H0919) (AP)

Gene Names

LEF1; LEF-1; TCF10; TCF7L3; TCF1ALPHA

Reactivity

Human

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (EHD3 monoclonal antibody. Western Blot analysis of EHD3 expression in COLO 320 HSR.)

WB (Western Blot) (EHD3 monoclonal antibody, Western Blot analysis of EHD3 expression in IMR-32.)

Application Data (Detection limit for recombinant GST tagged EHD3 is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to EHD3 on HeLa cell. [antibody concentration 25ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to EHD3 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3ug/ml].)

WB (Western Blot) (EHD3 monoclonal antibody. Western Blot analysis of EHD3 expression in MCF-7.)

WB (Western Blot) (Western Blot detection against Immunogen (31.61kD).)

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EHD3, Monoclonal Antibody (Cat# AAA24788)

Full Name

EHD3 (EH Domain-containing Protein 3, PAST Homolog 3, EHD2, PAST3) (Biotin)

Gene Names

EHD3; PAST3

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with p27 KIP 1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining p27 KIP 1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining p27 KIP 1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-p27 KIP 1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-p27 KIP 1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-p27 KIP 1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-p27 KIP 1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-p27 KIP 1 antibody. Counter stained with hematoxylin.)

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p27Kip1, Monoclonal Antibody (Cat# AAA30078)

Full Name

p27 KIP 1 Antibody

Gene Names

CDKN1B; KIP1; MEN4; CDKN4; MEN1B; P27KIP1

Reactivity

Human, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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WB (Western Blot) (HSPA1B monoclonal antibody Western Blot analysis of HSPA1B expression in Raw 264.7.)

WB (Western Blot) (HSPA1B monoclonal antibody Western Blot analysis of HSPA1B expression in NIH/3T3)

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between TP53 and HSPA1B. HeLa cells were stained with TP53 rabbit purified polyclonal 1:1200 and HSPA1B mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Application Data (Detection limit for recombinant GST tagged HSPA1B is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HSPA1B on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HSPA1B on formalin-fixed paraffin-embedded human testis. [antibody concentration 6ug/ml])

WB (Western Blot) (Western Blot detection against Immunogen (34.65kD).)

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HSPA1A, Monoclonal Antibody (Cat# AAA24241)

Full Name

HSPA1A (Heat Shock 70kD Protein 1A/1B, Heat Shock 70kD Protein 1/2, HSP70.1/HSP70.2, HSP70-1/HSP70-2, HSPA1, HSPA1B) (AP)

Gene Names

HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70.1; HSP70-1A; HEL-S-103

Reactivity

Human, Mouse, Rat

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (IL20 monoclonal antibody, Western Blot analysis of IL20 expression in K-562.)

Application Data (Detection limit for recombinant GST tagged IL20 is ~0.3ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of IL20 transfected lysate using IL20 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with IL20 rabbit polyclonal antibody.)

WB (Western Blot) (Western Blot analysis of IL20 expression in transfected 293T cell line by IL20 monoclonal antibody Lane 1: IL20 transfected lysate (20.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (IL20 monoclonal antibody. Western Blot analysis of IL20 expression in A-431.)

WB (Western Blot) (Western Blot detection against Immunogen (38.21kD).)

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Interleukin 20, Monoclonal Antibody (Cat# AAA24542)

Full Name

Interleukin 20 (Interleukin-20, IL-20, IL20, Cytokine Zcyto10, IL10D, MGC96907, UNQ852/PRO1801, ZCYTO10) APC

Gene Names

IL20; IL-20; IL10D; ZCYTO10

Reactivity

Human

Applications

EIA, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (CSNK2A1 monoclonal antibody, Western Blot analysis of CSNK2A1 expression in Hela NE.)

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between TP53 and CSNK2A1 HeLa cells were stained with TP53 rabbit purified polyclonal 1:1200 and CSNK2A1 mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Application Data (Detection limit for recombinant GST tagged CSNK2A1 is ~3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CSNK2A1 on HeLa cell. [antibody concentration 10ug/ml].)

WB (Western Blot) (Western Blot analysis of CSNK2A1 expression in transfected 293T cell line by CSNK2A1 monoclonal antibody. Lane 1: CSNK2A1 transfected lysate (45.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (36.63kD).)

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CSNK2A1, Monoclonal Antibody (Cat# AAA25067)

Full Name

CSNK2A1 (Casein Kinase II Subunit alpha, CK II alpha, CK2A1) (FITC)

Gene Names

CSNK2A1; CKII; CK2A1; OCNDS; CSNK2A3

Reactivity

Human

Applications

EIA, IF, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (CBS monoclonal antibody. Western Blot analysis of CBS expression in MCF-7.)

WB (Western Blot) (CBS monoclonal antibody, Western Blot analysis of CBS expression in HeLa.)

WB (Western Blot) (Western blot analysis of CBS over-expressed 293 cell line, cotransfected with CBS Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with 124401. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged CBS is ~0.1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of CBS transfected lysate using 124401and Protein A Magnetic Bead and immunoblotted with CBS rabbit polyclonal antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of formalin-fixed paraffin-embedded human hepatocellular carcinoma using 124401 (3ug/ml).)

WB (Western Blot) (Western Blot analysis of CBS expression in transfected 293T cell line by 124401. Lane 1: CBS transfected lysate (61kD). Lane 2: Non-transfected lysate.)

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CBS, Monoclonal Antibody (Cat# AAA25340)

Full Name

CBS (Cystathionine beta-synthase, Serine Sulfhydrase, Beta-thionase) (HRP)

Gene Names

CBS; HIP4

Reactivity

Human

Applications

EIA, IHC, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 IHC-p 1:50-300)

IHC (Immunohistchemistry) (Dilution: IF: 1:50-200 IHC-p 1:50-300)

IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 IHC-p 1:50-300)

IHC (Immunohistochemistry-Paraffin) (Dilution: IF: 1:50-200 IHC-p 1:50-300)

IHC (Immunohistochemistry-Paraffin) (Dilution: IF: 1:50-200 IHC-p 1:50-300)

IHC (Immunohistochemistry-Paraffin) (Dilution: IF: 1:50-200 IHC-p 1:50-300)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 IHC-p 1:50-300)

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Collagen I, Monoclonal Antibody (Cat# AAA28918)

Full Name

Collagen I mouse Monoclonal Antibody (4H10)

Gene Names

COL1A1; OI4

Reactivity

Human, Mouse, Rat

Applications

IHC-P, IF

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

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Application Data (Staining of mouse spleen cells with Rat anti Mouse CD4)

Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD4:FITC)

Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD4: Alexa Fluor 405 (AAA11938A405))

Application Data (Staining of mouse spleen with Rat anti Mouse CD4: Low Endotoxin)

Application Data (Staining of mouse spleen cells with Rat anti Mouse CD4:RPE)

Application Data (Staining of mouse spleen with Rat anti Mouse CD4)

Application Data (Staining of mouse spleen cells with Rat anti Mouse CD4: APC)

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CD4, Monoclonal Antibody (Cat# AAA11938)

Full Name

RAT ANTI MOUSE CD4

Gene Names

Cd4; L3T4; Ly-4

Applications

Immunohistochemistry, Flow Cytometry

Pricing

$600/0.25 mg $1,140/2x0.25 mg $1,650/3x0.25 mg $2,135/4x0.25 mg $2,385/5x0.25 mg

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WB (Western Blot) (Western Blot analysis of NDUFV2 expression in transfected 293T cell line by NDUFV2 monoclonal antibody. Lane 1: NDUFV2 transfected lysate (Predicted MW: 27.4kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (NDUFV2 monoclonal antibody Western Blot analysis of NDUFV2 expression in K-562.)

WB (Western Blot) (NDUFV2 monoclonal antibody Western Blot analysis of NDUFV2 expression in NIH/3T3.)

WB (Western Blot) (NDUFV2 monoclonal antibody Western Blot analysis of NDUFV2 expression in Raw 264.7.)

WB (Western Blot) (NDUFV2 monoclonal antibody Western Blot analysis of NDUFV2 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

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NDUFV2, Monoclonal Antibody (Cat# AAA24582)

Full Name

NDUFV2 (NADH Dehydrogenase [Ubiquinone] Flavoprotein 2, Mitochondrial, NADH-ubiquinone Oxidoreductase 24kD Subunit) APC

Gene Names

NDUFV2; CI-24k

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (CRYM monoclonal antibody, Western Blot analysis of CRYM expression in Jurkat.)

Application Data (Detection limit for recombinant GST tagged CRYM is ~0.03ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of CRYM transfected lysate using CRYM monoclonal antibody and Protein A Magnetic Bead and immunoblotted with CRYM rabbit polyclonal antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CRYM on formalin-fixed paraffin-embedded human skeletal muscle. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of CRYM expression in transfected 293T cell line by CRYM monoclonal antibody. Lane 1: CRYM transfected lysate (33.8kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

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CRYM, Monoclonal Antibody (Cat# AAA24473)

Full Name

CRYM (Thiomorpholine-carboxylate Dehydrogenase, Mu-crystallin Homolog, NADP-regulated Thyroid-hormone-binding Protein, Ketimine Reductase, THBP) APC

Gene Names

CRYM; THBP; DFNA40

Reactivity

Human

Applications

EIA, IHC, IP, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (NUP62 monoclonal antibody. Western Blot analysis of NUP62 expression in NIH/3T3.)

Application Data (Detection limit for recombinant GST tagged NUP62 is ~1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NUP62 on HeLa cell. [antibody concentration 10ug/ml])

WB (Western Blot) (NUP62 monoclonal antibody Western Blot analysis of NUP62 expression in Hela NE.)

WB (Western Blot) (NUP62 monoclonal antibody. Western Blot analysis of NUP62 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)

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NUP62, Monoclonal Antibody (Cat# AAA25774)

Full Name

NUP62 (Nuclear Pore Glycoprotein p62, 62kD Nucleoporin, Nucleoporin Nup62) (PE)

Gene Names

NUP62; p62; IBSN; SNDI

Reactivity

Human, Mouse, Rat

Applications

EIA, IF, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western Blot Analysis of Raji cell lysate using CD20 Mouse Monoclonal Antibody (L26).)

IF (Immunofluorescence) (Immunofluorescence staining of Raji cells using CD20 Mouse Monoclonal Antibody (L26) followed by goat anti-Mouse IgG conjugated to CF488 (green). Nuclei are stained with Reddot.)

SDS-PAGE (SDS-PAGE Analysis Purified CD20 Mouse Monoclonal Antibody (L26). Confirmation of Integrity and Purity of Antibody.)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Lymphoma stained with CD20 Mouse Monoclonal Antibody (L26).)

IF (Immunofluorescence) (Cytometric Analysis of Raji cells using CD20 Mouse Monoclonal Antibody (L26) followed by Goat anti-Mouse IgG-CF488 (Blue); Isotype Control (Red).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Lymphoma stained with CD20 Mouse Monoclonal Antibody (L26).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Tonsil stained with CD20 Mouse Monoclonal Antibody (L26).)

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CD20/MS4A1, Monoclonal Antibody (Cat# AAA23946)

Full Name

CD20/MS4A1 (B-Cell Marker)

Gene Names

MS4A1; B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16

Reactivity

Human

Applications

FC/FACS, IF, WB, IHC

Purity

Purified Ab with BSA and Azide at 200ug/ml OR Purified Ab WITHOUT BSA and Azide at 1.0mg/ml

Pricing

$295/0.02 mg (With BSA & Azide at 0.2mg/mL) $475/0.1 mg (With BSA & Azide at 0.2mg/mL) $475/0.1 mg (Without BSA & Azide at 1mg/mL) $905/2x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,310/3x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,690/4x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,900/5x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,900/5x0.1 mg (Without BSA & Azide at 1mg/mL)

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TESK2 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TESK2 on HeLa cell. [antibody concentration 10 ug/ml])

Application Data (Detection limit for recombinant GST tagged TESK2 is approximately 0.1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TESK2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.7 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TESK2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.7 ug/ml])

WB (Western Blot) (Western Blot analysis of TESK2 expression in transfected 293T cell line by TESK2 monoclonal antibody (M08), clone 5G1.Lane 1: TESK2 transfected lysate(60.3 KDa).Lane 2: Non-transfected lysate.)

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TESK2, Monoclonal Antibody (Cat# AAA26049)

Full Name

TESK2 (Testis-Specific Kinase 2) (APC)

Applications

IF, IHC, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (RUNX1 monoclonal antibody Western Blot analysis of RUNX1 expression in Hela NE.)

Application Data (Detection limit for recombinant GST tagged RUNX1 is ~1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RUNX1 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RUNX1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml].)

WB (Western Blot) (RUNX1 monoclonal antibody Western Blot analysis of RUNX1 expression in NIH/3T3.)

WB (Western Blot) (Western Blot detection against Immunogen (36.85kD).)

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RUNX1, Monoclonal Antibody (Cat# AAA25530)

Full Name

RUNX1 (Runt-related Transcription Factor 1, Acute Myeloid Leukemia 1 Protein, Core-binding Factor Subunit alpha-2, CBF-alpha-2, Oncogene AML-1, Polyomavirus Enhancer-binding Protein 2 alpha B Subunit, PEA2-alpha B, PEBP2-alpha B, SL3-3 Enhancer Factor 1 a

Gene Names

RUNX1; AML1; CBFA2; EVI-1; AMLCR1; PEBP2aB; AML1-EVI-1

Reactivity

Human, Mouse

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Detection of human Phospho KAP-1 (S824) by western blot. Samples: Whole cell lysate (25 ug) from HEK293T cells treated with 100 uM etoposide (+) or mock treated (-) prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal antibody [BL-246-7B5] (AAA23791 lot 4) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-KAP1 recombinant monoclonal antibody [BL-248-2G6] .)

WB (Western Blot) (Detection of mouse Phospho KAP-1 (S824) by western blot. Samples: Whole cell lysate (50 ug) from NIH3T3 cells treated with 100 uM etoposide (+) or mock treated (-) prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal antibody [BL-246-7B5] (AAA23791 lot 4) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-KAP1 recombinant monoclonal antibody [BL-248-2G6] .)

Simple Western (Detection of human Phospho KAP-1 (S824) by Simple Western. Samples: Whole cell lysate (0.4 mg/mL) from HEK293T cells treated with 100 uM etoposide prepared using NETN lysis buffer. Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal antibody [BL-246-7B5] (AAA23791) used at 1:10, 1:50, and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram.)

IP (Immunoprecipitation) (Detection of human Phospho KAP-1 (S824) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from HEK293T cells prepared using NETN lysis buffer that were treated with 100 uM etoposide (+) or mock treated (-). Antibodies: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal antibody [BL-246-7B5] (AAA23791 lot 4) used for IP at 20 ul/mg lysate. Phospho KAP-1 (S824) was also immunoprecipitated by a previous lot of this antibody (AAA23791 lot 3) and rabbit anti-KAP-1 recombinant monoclonal antibody [BL-248-2G6] . For blotting immunoprecipitated Phospho KAP-1 (S824), AAA23791 was used at 1:1000. Chemiluminescence with an exposure time of 1 second. Lower Panel: Rabbit anti-KAP1 recombinant monoclonal antibody [BL-248-2G6] .)

IHC (Immunohistochemistry) (Detection of human Phospho KAP-1 (S824) in FFPE prostate carcinoma by IHC. Mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right). Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal [BL-246-7B5] (AAA23791). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry) (Detection of human Phospho KAP-1 (S824) by immunocytochemistry. Samples: Formaldehyde-fixed asynchronous HeLa cells grown in chambered microscope slides and treated with etoposide (right) or untreated (left). Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal antibody [BL-246-7B5] (AAA23791-T lot 1) used at of 1:100. Secondary: DyLight 594-conjugated goat anti-rabbit IgG . Counterstain: Phalloidin conjugated Alexa Fluor 488 (green).)

ICC (Immunocytochemistry) (Detection of human Phospho KAP-1 (S824) in FFPE etoposide treated HeLa cells by ICC. Mock phosphatase treated section (left) and calf intestinal phosphatase-treated section (right). Antibody: Rabbit anti-Phospho KAP-1 (S824) recombinant monoclonal [BL-246-7B5] (AAA23791). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

FCM (Flow Cytometry) (Detection of human phospho KAP-1 (shaded) in etoposide treated HEK293T cells (right) and untreated HEK293T cells (left) by flow cytometry. Antibody: Rabbit anti-phospho KAP-1 recombinant monoclonal [BL-246-7B5] (AAA23791) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)

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KAP-1, Monoclonal Recombinant Antibody (Cat# AAA23791)

Full Name

Rabbit anti-Phospho KAP-1 (S824) Recombinant Monoclonal Antibody [BL-246-7B5]

Gene Names

TRIM28; KAP1; TF1B; RNF96; TIF1B; PPP1R157

Reactivity

Human, Mouse

Applications

WB, IP, IHC, ICC-IF, FC/FACS, SW-Size

Purity

Purified

Pricing

$295/0.02 mL $480/0.1 mL $915/2x0.1 mL $980/5x0.02 mL $1,325/3x0.1 mL $1,705/4x0.1 mL $1,855/5x0.1 mL

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Application Data (Proximity Ligation Analysis of protein-protein interactions between MSH2 and MLH1 HeLa cells were stained with anti-MSH2 rabbit purified polyclonal 1:1200 and anti-MLH1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)

Application Data (Detection limit for recombinant GST tagged MLH1 is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MLH1 on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to MLH1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 1ug/ml])

WB (Western Blot) (MLH1 monoclonal antibody Western Blot analysis of MLH1 expression in HeLa NE.)

WB (Western Blot) (Western Blot detection against Immunogen (108.9kD).)

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MLH1, Monoclonal Antibody (Cat# AAA24573)

Full Name

MLH1 (MutL Homolog 1, MutL Protein Homolog 1, COCA2, DNA Mismatch Repair Protein Mlh1, FCC2, hMLH1, HNPCC, HNPCC2, MGC5172, MutL Homolog 1 Colon Cancer Nonpolyposis Type 2) APC

Gene Names

MLH1; FCC2; COCA2; HNPCC; hMLH1; HNPCC2

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (EIF2S1 monoclonal antibody, Western Blot analysis of EIF2S1 expression in HeLa.)

WB (Western Blot) (EIF2S1 monoclonal antibody. Western Blot analysis of EIF2S1 expression in Hela NE.)

WB (Western Blot) (Western blot analysis of EIF2S1 over-expressed 293 cell line, cotransfected with EIF2S1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with EIF2S1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged EIF2S1 is ~0.3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to EIF2S1 on HeLa cell. [antibody concentration 10ug/ml].)

WB (Western Blot) (Western Blot analysis of EIF2S1 expression in transfected 293T cell line by EIF2S1 monoclonal antibody. Lane 1: EIF2S1 transfected lysate (36.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (60.39kD).)

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EIF2S1, Monoclonal Antibody (Cat# AAA25086)

Full Name

EIF2S1 (Eukaryotic Translation Initiation Factor 2, Subunit 1 alpha, eIF2 alpha, eIF-2-alpha, eIF-2A, eIF-2alpha, EIF2A, Eukaryotic Translation Initiation Factor 2 Subunit 1) (FITC)

Gene Names

EIF2S1; EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha

Reactivity

Human

Applications

EIA, IF, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (TIMM9 monoclonal antibody. Western Blot analysis of TIMM9 expression in Raw 264.7.)

WB (Western Blot) (TIMM9 monoclonal antibody Western Blot analysis of TIMM9 expression in IMR-32.)

Application Data (Detection limit for recombinant GST tagged TIMM9 is ~0.1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TIMM9 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of TIMM9 expression in transfected 293T cell line by TIMM9 monoclonal antibody. Lane 1: TIMM9 transfected lysate (10.4kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (TIMM9 monoclonal antibody. Western Blot analysis of TIMM9 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (35.9kD).)

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TIMM9, Monoclonal Antibody (Cat# AAA24387)

Full Name

TIMM9 (Mitochondrial Import Inner Membrane Translocase Subunit Tim9, TIM9, TIM9A, TIMM9A) (AP)

Gene Names

TIMM9; TIM9; TIM9A

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of MCF-7 cells with mSin3A antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)

ICC (Immunocytochemistry) (ICC staining mSin3A in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining mSin3A in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining mSin3A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-mSin3A antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of mSin3A on 293T cell using anti-mSin3A antibody at 1/1, 000 dilution.)

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mSin3A, Monoclonal Antibody (Cat# AAA30362)

Full Name

mSin3A Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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WB (Western Blot) (FUSIP1 monoclonal antibody Western Blot analysis of FUSIP1 expression in Hela NE.)

Application Data (Detection limit for recombinant GST tagged FUSIP1 is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FUSIP1 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FUSIP1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of FUSIP1 expression in transfected 293T cell line by FUSIP1 monoclonal antibody Lane 1: FUSIP1 transfected lysate (22.2kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)

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FUSIP1, Monoclonal Antibody (Cat# AAA24808)

Full Name

FUSIP1 (SRSF10, FUSIP1, FUSIP2, SFRS13A, TASR, Serine/Arginine-rich Splicing Factor 10, 40kD SR-repressor Protein, FUS-interacting Serine-arginine-rich Protein 1, Splicing Factor SRp38, Splicing Factor, Arginine/Serine-rich 13A, TLS-associated Protein wit

Gene Names

SRSF10; NSSR; TASR; SRp38; TASR1; TASR2; FUSIP1; FUSIP2; SFRS13; SRrp40; SFRS13A; PPP1R149

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged TCF7L2 is approximately 0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TCF7L2 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TCF7L2 on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (TCF7L2 monoclonal antibody (M06), clone 3A4 Western Blot analysis of TCF7L2 expression in K-562 (Cat # L009V1).)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TCF7L2 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to TCF7L2 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml])

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TCF7L2, Monoclonal Antibody (Cat# AAA26354)

Full Name

TCF7L2 (Transcription Factor 7-like 2 (T-Cell Specific, HMG-Box), TCF-4, TCF4) (FITC)

Gene Names

TCF7L2; TCF4; TCF-4

Applications

IF, IHC, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to POU5F1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to POU5F1 on HeLa cell. [antibody concentration 10 ug/ml])

Application Data (A-549 cells were stained with POU5F1-FITC labeled monoclonal antibody (Green). The cell nucleus were counterstained with DAPI (Blue).)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to POU5F1 on A-549 cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (Western Blot analysis of POU5F1 expression in transfected 293T cell line by POU5F1 monoclonal antibody (M05), clone 1B11.Lane 1: POU5F1 transfected lysate (18.3 KDa).Lane 2: Non-transfected lysate.)

WB (Western Blot) (POU5F1 monoclonal antibody (M05), clone 1B11 Western Blot analysis of POU5F1 expression in HepG2 (Cat # L019V1).)

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POU5F1, Monoclonal Antibody (Cat# AAA26362)

Full Name

POU5F1 (POU Class 5 Homeobox 1, MGC22487, OCT3, OCT4, OTF3, OTF4) (FITC)

Applications

EIA, IF, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IP (Immunoprecipitation) (Immunoprecipitation of GART transfected lysate using GART monoclonal antibody and Protein A Magnetic Bead and immunoblotted with GART rabbit polyclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GART on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GART on formalin-fixed paraffin-embedded human endometrium tissue. [antibody concentration 2ug/ml].)

WB (Western Blot) (Western Blot analysis of GART expression in transfected 293T cell line by GART monoclonal antibody. Lane 1: GART transfected lysate (46kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (GART monoclonal antibody, Western Blot analysis of GART expression in HeLa NE.)

WB (Western Blot) (Western Blot detection against Immunogen (73.37kD).)

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GART, Monoclonal Antibody (Cat# AAA25402)

Full Name

GART (Trifunctional Purine Biosynthetic Protein Adenosine-3, PGFT, PRGS, MGC47764) (HRP)

Gene Names

GART; AIRS; GARS; PAIS; PGFT; PRGS; GARTF

Reactivity

Human

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western blot analysis of NMI over-expressed 293 cell line, cotransfected with NMI Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NMI monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged NMI is ~0.03ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NMI on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of NMI expression in transfected 293T cell line by NMI monoclonal antibody. Lane 1: NMI transfected lysate (35.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (NMI monoclonal antibody Western Blot analysis of NMI expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)

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NMI, Monoclonal Antibody (Cat# AAA25473)

Full Name

NMI (N-myc-interactor, Nmi, N-myc and STAT Interactor) (HRP)

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged RPS23 is ~0.3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RPS23 on HeLa cell. [antibody concentration 10ug/ml])

WB (Western Blot) (RPS23 monoclonal antibody. Western Blot analysis of RPS23 expression in NIH/3T3.)

WB (Western Blot) (RPS23 monoclonal antibody. Western Blot analysis of RPS23 expression in Raw 264.7.)

WB (Western Blot) (RPS23 monoclonal antibody. Western Blot analysis of RPS23 expression in PC-12.)

WB (Western Blot) (RPS23 monoclonal antibody, Western Blot analysis of RPS23 expression in HeLa.)

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RPS23, Monoclonal Antibody (Cat# AAA24935)

Full Name

RPS23 (40S Ribosomal Protein S23, FLJ35016) (Biotin)

Gene Names

RPS23; S23; BTDD; uS12; MABAS; MCINS; PAMAS

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged UBTF is approximately 1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to UBTF on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to UBTF on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])

WB (Western Blot) (UBTF monoclonal antibody (M04), clone 2D8 Western Blot analysis of UBTF expression in HepG2 (Cat # L019V1).)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to UBTF on HepG2 cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to UBTF on HepG2 cell. [antibody concentration 10 ug/ml])

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UBTF, Monoclonal Antibody (Cat# AAA26370)

Full Name

UBTF (Upstream Binding Transcription Factor, RNA Polymerase I, NOR-90, UBF) (FITC)

Gene Names

UBTF; UBF; UBF1; UBF2; UBF-1; CONDBA; NOR-90

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged SMARCB1 is ~0.03ng/ml as a capture antibody.)

IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to SMARCB1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml])

WB (Western Blot) (SMARCB1 monoclonal antibody. Western Blot analysis of SMARCB1 expression in NIH/3T3.)

WB (Western Blot) (SMARCB1 monoclonal antibody. Western Blot analysis of SMARCB1 expression in Raw 264.7.)

WB (Western Blot) (SMARCB1 monoclonal antibody, Western Blot analysis of SMARCB1 expression in Hela NE.)

WB (Western Blot) (SMARCB1 monoclonal antibody, Western Blot analysis of SMARCB1 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)

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SMARCB1, Monoclonal Antibody (Cat# AAA25253)

Full Name

SMARCB1 (SWI/SNF-related Matrix-associated Actin-dependent Regulator of Chromatin Subfamily B Member 1, BRG1-associated Factor 47, BAF47, Integrase Interactor 1 Protein, SNF5 Homolog, hSNF5, BAF47, INI1, SNF5L1) (FITC)

Gene Names

SMARCB1; RDT; CSS3; INI1; SNF5; Snr1; BAF47; MRD15; RTPS1; Sfh1p; hSNFS; SNF5L1; SWNTS1; PPP1R144

Reactivity

Human, Mouse, Rat

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of TRIM28 expression in transfected 293T cell line by TRIM28 monoclonal antibody (M02), clone 1D11.Lane 1: TRIM28 transfected lysate (Predicted MW: 88.5 KDa).Lane 2: Non-transfected lysate.)

WB (Western Blot) (TRIM28 monoclonal antibody (M02), clone 1D11. Western Blot analysis of TRIM28 expression in MCF-7.)

WB (Western Blot) (TRIM28 monoclonal antibody (M02), clone 1D11. Western Blot analysis of TRIM28 expression in PC-12 (Cat # L012V1).)

WB (Western Blot) (TRIM28 monoclonal antibody (M02), clone 1D11. Western Blot analysis of TRIM28 expression in NIH/3T3 (Cat # L018V1).)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TRIM28 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to TRIM28 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml])

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TRIM28, Monoclonal Antibody (Cat# AAA26579)

Full Name

TRIM28 (Tripartite Motif-Containing 28, FLJ29029, KAP1, RNF96, TF1B, TIF1B) (PE)

Gene Names

TRIM28; KAP1; TF1B; RNF96; TIF1B; PPP1R157

Applications

EIA, IHC, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged ADRM1 is ~0.1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of ADRM1 transfected lysate using ADRM1 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with ADRM1 monoclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ADRM1 on HeLa cell. [antibody concentration 10ug/ml].)

WB (Western Blot) (Western Blot analysis of ADRM1 expression in transfected 293T cell line by ADRM1 monoclonal antibody Lane 1: ADRM1 transfected lysate (42.2kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (ADRM1 monoclonal antibody Western Blot analysis of ADRM1 expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (69.01kD).)

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ADRM1, Monoclonal Antibody (Cat# AAA25308)

Full Name

ADRM1 (GP110, Proteasomal Ubiquitin Receptor ADRM1, 110kD Cell Membrane Glycoprotein, Adhesion-regulating Molecule 1, Proteasome Regulatory Particle Non-ATPase 13, Rpn13 Homolog, MGC29536) (HRP)

Gene Names

ADRM1; ARM1; ARM-1; GP110

Reactivity

Human

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

What are Monoclonal Antibodies?

Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.

This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.

AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.

What Are The Uses of Monoclonal Antibodies

Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.

ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.

Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.

Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.

Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.

How Monoclonal Antibodies Are Used as Medicine?

Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).

Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:

• Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.


• Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.


• Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.

Why Buy Monoclonal Antibodies From Us?

At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.

1. Validated for Versatile Applications

The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).

2. Wide Selection & Specialized Options

We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.

3. High-Quality Proteins

Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.

Frequently Asked Questions

1. Are your monoclonal antibodies validated for specific applications?

Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.

2. How do I choose the right monoclonal antibody for my application?

Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.

3. How quickly can I receive my order?

Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.