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IF (Immunofluorescence) (Immunofluorescence analysis of Mouse-lung tissue. 1.EFHD1 Monoclonal Antibody(3G2)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IF (Immunofluorescence) (IF analysis of Hela with antibody (Left) and DAPI (Right) diluted at 1:100.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Rat-heart tissue. 1.EFHD1 Monoclonal Antibody(3G2) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Mouse-testis tissue. 1.EFHD1 Monoclonal Antibody(3G2) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1.EFHD1 Monoclonal Antibody(3G2) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
WB (Western Blot) (Western blot analysis of 1) Mouse spleen tissue. 2) Rat spleen tissue. diluted at 1:3000.)
Application Data (Detection limit for recombinant GST tagged HIP1 is ~0.3ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of HIP1 transfected lysate using HIP1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HIP1 rabbit polyclonal antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HIP1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of HIP1 expression in transfected 293T cell line by HIP1 monoclonal antibody Lane 1: HIP1 transfected lysate (116.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (HIP1 monoclonal antibody Western Blot analysis of HIP1 expression in HeLa)
WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD107b : Biotin . Permeabilised with Leucoperm)
Application Data (Staining of permeabilised mouse peritoneal macrophages with Rat anti Mouse CD107b: FITC)
Application Data (Staining of mouse peritoneal macrophages with Rat anti MouseCD107b : Alexa Fluor 647 . Permeabilised with Leucoperm)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD1076 following permeabilsation with Leucoperm)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD107b : FITC following permeabilisation with Leucoperm)
Application Data (Western blot analysis of CD107b on J774 cell lysate probed with Rat anti Mouse CD107b at 1/500, 1/1000, 1/2000. Rat anti tubulin alpha is included as a loading control. Detection is with Goat anti Rat IgG:Dylight®)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD107b : Alexa Fluor 488 . Permeabilised with Leucoperm)
FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of Caco-2 cells using anti-HGS antibody (AAA19679).Overlay histogram showing Caco-2 cells stained with AAA19679 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-HGS Antibody (AAA19679, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
IF (Immunofluorescence) (Figure 9. IF analysis of HGS using anti-HGS antibody (AAA19679).HGS was detected in an immunocytochemical section of U87 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL mouse anti-HGS Antibody (AAA19679) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)
IHC (Immunohistochemistry) (Figure 8. IHC analysis of HGS using anti-HGS antibody (AAA19679).HGS was detected in a paraffin-embedded section of mouse testis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-HGS Antibody (AAA19679) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)
IHC (Immunohistochemistry) (Figure 7. IHC analysis of HGS using anti-HGS antibody (AAA19679).HGS was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-HGS Antibody (AAA19679) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)
IHC (Immunohistchemistry) (Figure 6. IHC analysis of HGS using anti-HGS antibody (AAA19679).HGS was detected in a paraffin-embedded section of human laryngeal squamous cell carcinomas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-HGS Antibody (AAA19679) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)
IHC (Immunohistochemistry) (Figure 5. IHC analysis of HGS using anti-HGS antibody (AAA19679).HGS was detected in a paraffin-embedded section of human endometrial cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-HGS Antibody (AAA19679) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)
IHC (Immunohistochemistry) (Figure 4. IHC analysis of HGS using anti-HGS antibody (AAA19679).HGS was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-HGS Antibody (AAA19679) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)
IHC (Immunohistochemistry) (Figure 3. IHC analysis of HGS using anti-HGS antibody (AAA19679).HGS was detected in a paraffin-embedded section of human breast infiltrating ductal carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-HGS Antibody (AAA19679) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)
IHC (Immunohistochemistry) (Figure 2. IHC analysis of HGS using anti-HGS antibody (AAA19679).HGS was detected in a paraffin-embedded section of human bladder epithelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-HGS Antibody (AAA19679) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)
WB (Western Blot) (Figure 1. Western blot analysis of HGS using anti-HGS antibody (AAA19679).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human 293T whole cell lysates,Lane 3: human K562 whole cell lysates,Lane 4: human SK-OV-3 whole cell lysates,Lane 5: rat brain tissue lysates,Lane 6: rat PC-12 whole cell lysates,Lane 7: mouse brain tissue lysates,Lane 8: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-HGS antigen affinity purified monoclonal antibody (#AAA19679) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for HGS at approximately 110 kDa. The expected band size for HGS is at 110 kDa.)
Application Data (Detection limit for recombinant GST tagged NR3C1 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NR3C1 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of NR3C1 expression in transfected 293T cell line by NR3C1 monoclonal antibody. Lane 1: NR3C1 transfected lysate (85.7kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (NR3C1 monoclonal antibody, Western Blot analysis of NR3C1 expression in HeLa NE.)
WB (Western Blot) (NR3C1 monoclonal antibody. Western Blot analysis of NR3C1 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)
WB (Western Blot) (HSPA1B monoclonal antibody Western Blot analysis of HSPA1B expression in Raw 264.7.)
WB (Western Blot) (HSPA1B monoclonal antibody Western Blot analysis of HSPA1B expression in NIH/3T3)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between TP53 and HSPA1B. HeLa cells were stained with TP53 rabbit purified polyclonal 1:1200 and HSPA1B mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Application Data (Detection limit for recombinant GST tagged HSPA1B is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HSPA1B on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HSPA1B on formalin-fixed paraffin-embedded human testis. [antibody concentration 6ug/ml])
WB (Western Blot) (Western Blot detection against Immunogen (34.65kD).)
WB (Western Blot) (RIPK2 monoclonal antibody (M02), clone 6F7. Western Blot analysis of RIPK2 expression in PC-12 (Cat # L012V1).)
WB (Western Blot) (RIPK2 monoclonal antibody (M02), clone 6F7. Western Blot analysis of RIPK2 expression in NIH/3T3 (Cat # L018V1).)
Application Data (Detection limit for recombinant GST tagged RIPK2 is approximately 0.1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RIPK2 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 1.2 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RIPK2 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 1.2 ug/ml])
WB (Western Blot) (RIPK2 monoclonal antibody (M02), clone 6F7 Western Blot analysis of RIPK2 expression in HeLa (Cat # L013V1).)
Application Data (Detection limit for recombinant GST tagged RUNX1 is approximately 1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RUNX1 on HeLa cell. [antibody concentration 40 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RUNX1 on HeLa cell. [antibody concentration 40 ug/ml])
WB (Western Blot) (RUNX1 monoclonal antibody (M06), clone 2C10 Western Blot analysis of RUNX1 expression in Hela S3 NE (Cat # L013V3).)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RUNX1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1.5 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to RUNX1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1.5 ug/ml])
WB (Western Blot) (RIPK2 monoclonal antibody (M02), clone 6F7. Western Blot analysis of RIPK2 expression in PC-12 (Cat # L012V1).)
WB (Western Blot) (RIPK2 monoclonal antibody (M02), clone 6F7. Western Blot analysis of RIPK2 expression in NIH/3T3 (Cat # L018V1).)
Application Data (Detection limit for recombinant GST tagged RIPK2 is approximately 0.1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RIPK2 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 1.2 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RIPK2 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 1.2 ug/ml])
WB (Western Blot) (RIPK2 monoclonal antibody (M02), clone 6F7 Western Blot analysis of RIPK2 expression in HeLa (Cat # L013V1).)
WB (Western Blot) (Detection of human 53BP1 by western blot. Samples: Whole cell lysate (15 ug) from HeLa, HEK293T, MCF-7, Hep-G2, A-549, SW620, SK-MEL-28 (SK), and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (AAA23789 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 second.)
IP (Immunoprecipitation) (Detection of human 53BP1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HEK293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (AAA23789 lot 1) used for IP at 20 ul per reaction. 53BP1 was also immunoprecipitated by rabbit anti-53BP1 antibody . For blotting immunoprecipitated 53BP1, AAA23789 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 second.)
IHC (Immunohistochemistry) (Detection of mouse 53BP1 by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (AAA23789 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry) (Detection of human 53BP1 by immunohistochemistry. Sample: FFPE section of colon carcinoma. Antibody: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (AAA23789 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
FCM (Flow Cytometry) (Detection of mouse 53BP1 (shaded) in mIMCD-3 cells by flow cytometry. Antibody: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (AAA23789) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry) (Detection of human 53BP1 (shaded) in HDLM-2 cells by flow cytometry. Antibody: Rabbit anti-53BP1 recombinant monoclonal antibody [BL-250-1H11] (AAA23789) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
WB (Western Blot) (ASB9 monoclonal antibody, Western Blot analysis of ASB9 expression in HepG2.)
Application Data (Detection limit for recombinant GST tagged ASB9 is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of ASB9 transfected lysate using ASB9 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with ASB9 rabbit polyclonal antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ASB9 on formalin-fixed paraffin-embedded human lymphoma tissue. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of ASB9 expression in transfected 293T cell line by ASB9 monoclonal antibody. Lane 1: ASB9 transfected lysate (31.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (53.83kD).)
WB (Western Blot) (Detection of human CD74 by western blot. Samples: Whole cell lysate (50 ug) from HEK293T, Raji, GaMG, HeLa, SR, Jurkat, Daudi, and KG-1 cells prepared using NETN lysis buffer. Antibody: Mouse anti-CD74 monoclonal antibody [LN2] (AAA23781 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-RPS3 .)
IHC (Immunohistochemistry) (Detection of human CD74 in FFPE colon carcinoma by IHC. Antibody: Mouse monoclonal anti-CD74 [LN2] (AAA23781 lot 1). Secondary: HRP-conjugated goat anti-mouse IgG . Substrate: DAB.)
IHC (Immunohistochemistry) (Detection of human CD74 in FFPE lymph node by IHC. Antibody: Mouse monoclonal anti-CD74 [LN2] (AAA23781 lot 1). Secondary: HRP-conjugated goat anti-mouse IgG . Substrate: DAB.)
ICC (Immunocytochemistry) (Detection of human CD74 in FFPE Raji cells by ICC. Antibody: Mouse monoclonal anti-CD74 [LN2] (AAA23781 lot 1). Secondary: HRP-conjugated goat anti-mouse IgG . Substrate: DAB.)
ICC (Immunocytochemistry) (Detection of human CD74 in FFPE Daudi cells by ICC. Antibody: Mouse monoclonal anti-CD74 [LN2] (AAA23781 lot 1). Secondary: HRP-conjugated goat anti-mouse IgG . Substrate: DAB.)
FCM (Flow Cytometry) (Detection of human CD74 (shaded) in Ramos cells by flow cytometry. Antibody: Mouse anti-CD74 recombinant monoclonal [LN2] (AAA23781) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-mouse IgG .)
WB (Western Blot) (NEK9 monoclonal antibody. Western Blot analysis of NEK9 expression in PC-12.)
WB (Western Blot) (NEK9 monoclonal antibody Western Blot analysis of NEK9 expression in HeLa.)
Application Data (Detection limit for recombinant GST tagged NEK9 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NEK9 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of NEK9 expression in transfected 293T cell line by NEK9 monoclonal antibody. Lane 1: NEK9 transfected lysate (107.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (NEK9 monoclonal antibody. Western Blot analysis of NEK9 expression in NIH/3T3.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PRKDC on HeLa cell. [antibody concentration 40 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PRKDC on HeLa cell. [antibody concentration 40 ug/ml])
Application Data (Detection limit for recombinant GST tagged PRKDC is 1 ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PRKDC on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PRKDC on formalin-fixed paraffin-embedded human liver. [antibody concentration 3 ug/ml])
WB (Western Blot) (PRKDC monoclonal antibody (M03), clone 2A8 Western Blot analysis of PRKDC expression in Hela S3 NE.)
WB (Western Blot) (CDC2 monoclonal antibody, Western Blot analysis of CDC2 expression in Hela.)
Application Data (Detection limit for recombinant GST tagged CDC2 is ~30ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CDC2 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CDC2 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 0.3ug/ml].)
WB (Western Blot) (Western Blot analysis of CDC2 expression in transfected 293T cell line by CDC2 monoclonal antibody. Lane 1: CDC2 transfected lysate (34.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (58.41kD).)
WB (Western Blot) (Detection of human HLA-DR by western blot. Samples: Whole cell lysate (50 ug) from KG-1, SNB-75, Raji, Ramos, and SR (10 ug) cells prepared using NETN lysis buffer. Antibody: Mouse anti-HLA-DR monoclonal antibody [LN3] (AAA23780 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-mouse IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
IHC (Immunohistochemistry) (Detection of human HLA-DR (red) by immunohistochemistry. Sample: FFPE section of human tonsil. Antibody: Mouse anti-HLA-DR monoclonal antibody [LN3] (AAA23780 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-Mouse IgG . Substrate: Opal. Counterstain: DAPI (blue).)
IHC (Immunohistochemistry) (Detection of human HLA-DR by immunhistochemistry. Sample: FFPE section of human tonsil. Antibody: Mouse monoclonal anti-HLA-DR antibody [LN3] (AAA23780 lot 1) used at 1:100. Secondary: DyLight 594-conjugated goat anti-mouse IgG .)
IHC (Immunohistochemistry) (Detection of human HLA-DR by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Mouse anti-HLA-DR monoclonal antibody [LN3] (AAA23780-2). Secondary: HRP-conjugated goat anti-mouse IgG .)
ICC (Immunocytochemistry) (Detection of human HLA-DR by immunocytochemistry. Sample: FFPE section of SR cells. Antibody: Mouse anti-HLA-DR monoclonal antibody [LN3] (AAA23780-2). Secondary: HRP-conjugated goat anti-mouse IgG .)
FCM (Flow Cytometry) (Detection of human HLA-DR (shaded) in Daudi cells by flow cytometry. Antibody: Mouse anti-HLA-DR monoclonal antibody [LN3] (AAA23780) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-mouse IgG .)
FCM (Flow Cytometry) (Flow cytometric analysis of HepG2 cells with Glutathione Synthetase antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining Glutathione Synthetase in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Glutathione Synthetase antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-Glutathione Synthetase antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Glutathione Synthetase antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Glutathione Synthetase antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Glutathione Synthetase antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Glutathione Synthetase on different lysates using anti-Glutathione Synthetase antibody at 1/500 dilution. Positive control: Lane 1: SiHa Lane 2: Mouse colon Lane 3: Rat liver)
WB (Western Blot) (SAV1 monoclonal antibody (M02), Western Blot analysis of SAV1 expression in Hela NE.)
Application Data (Detection limit for recombinant GST tagged SAV1 is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of SAV1 transfected lysate using SAV1 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SAV1 on HeLa cell. [antibody concentration 60ug/ml].)
WB (Western Blot) (SAV1 monoclonal antibody. Western Blot analysis of SAV1 expression in HepG2.)
WB (Western Blot) (Western Blot detection against Immunogen (35.35kD).)
Application Data (Detection limit for recombinant GST tagged MPG is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MPG on HeLa cell. [antibody concentration 40ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to MPG on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of MPG expression in transfected 293T cell line by MPG monoclonal antibody. Lane 1: MPG transfected lysate (32.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (MPG monoclonal antibody Western Blot analysis of MPG expression in Hela NE.)
WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)
Application Data (Detection limit for recombinant GST tagged PTBP1 is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of PTBP1 transfected lysate using PTBP1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PTBP1 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PTBP1 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PTBP1 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of PTBP1 expression in transfected 293T cell line by PTBP1 monoclonal antibody, Lane 1: PTBP1 transfected lysate (59.6kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (PTBP1 monoclonal antibody, Western Blot analysis of PTBP1 expression in A-431.)
PTBP1 (Polypyrimidine Tract-binding Protein 1, PTB, Heterogeneous Nuclear Ribonucleoprotein I, hnRNP I, 57kD RNA-binding Protein PPTB-1, MGC10830, MGC8461) (HRP)
Application Data (Detection limit for recombinant GST tagged CSE1L is approximately 0.1ng/ml as a capture antibody.)
WB (Western Blot) (CSE1L monoclonal antibody (M05), clone 3F8 Western Blot analysis of CSE1L expression in K-562.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CSE1L on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CSE1L on HeLa cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CSE1L on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to CSE1L on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml])
WB (Western Blot) (Western blot analysis of OCRL over-expressed 293 cell line, cotransfected with OCRL Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with OCRL monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged OCRL is ~0.1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to OCRL on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of OCRL expression in transfected 293T cell line by OCRL monoclonal antibody Lane 1: OCRL transfected lysate (103.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (OCRL monoclonal antibody Western Blot analysis of OCRL expression in Hela NE.)
WB (Western Blot) (Western Blot detection against Immunogen (36.08kD).)
Application Data (Proximity Ligation Analysis of protein-protein interactions between MSH2 and MLH1 HeLa cells were stained with anti-MSH2 rabbit purified polyclonal 1:1200 and anti-MLH1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
Application Data (Detection limit for recombinant GST tagged MLH1 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MLH1 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to MLH1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 1ug/ml])
WB (Western Blot) (MLH1 monoclonal antibody Western Blot analysis of MLH1 expression in HeLa NE.)
WB (Western Blot) (Western Blot detection against Immunogen (108.9kD).)
WB (Western Blot) (SLC25A13 monoclonal antibody Western Blot analysis of SLC25A13 expression in HepG2)
WB (Western Blot) (Western blot analysis of SLC25A13 over-expressed 293 cell line, cotransfected with SLC25A13 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with SLC25A13 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged SLC25A13 is ~0.3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SLC25A13 on HepG2 cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of SLC25A13 expression in transfected 293T cell line by SLC25A13 monoclonal antibody Lane 1: SLC25A13 transfected lysate (74.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (34.8kD).)
Application Data (Detection limit for recombinant GST tagged CSE1L is approximately 3ng/ml as a capture antibody.)
WB (Western Blot) (CSE1L monoclonal antibody (M08), clone 3D8 Western Blot analysis of CSE1L expression in Hela S3 NE (Cat # L013V3).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CSE1L on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CSE1L on HeLa cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CSE1L on formalin-fixed paraffin-embedded human prostate. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to CSE1L on formalin-fixed paraffin-embedded human prostate. [antibody concentration 3 ug/ml])
WB (Western Blot) (ACATE2 monoclonal antibody Western Blot analysis of ACATE2 expression in MCF-7.)
Application Data (Detection limit for recombinant GST tagged ACOT9 is ~3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ACOT9 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ACOT9 on formalin-fixed paraffin-embedded human heart. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of ACOT9 expression in transfected 293T cell line by ACATE2 monoclonal antibody. Lane 1: ACOT9 transfected lysate (24kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (49.43kD).)
WB (Western Blot) (RNF12 monoclonal antibody, Western Blot analysis of RNF12 expression in HeLa NE.)
Application Data (Detection limit for recombinant GST tagged RNF12 is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of RNF12 transfected lysate using RNF12 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with RNF12 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RNF12 on HeLa cell. [antibody concentration 30ug/ml].)
WB (Western Blot) (Western Blot analysis of RNF12 expression in transfected 293T cell line by RNF12 monoclonal antibody. Lane 1: RNF12 transfected lysate (68.5kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (35.24kD).)
WB (Western Blot) (Western Blot detection against Immunogen (84.7kD).)
WB (Western Blot) (Western blot analysis of LCK over-expressed 293 cell line, cotransfected with LCK Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with LCK monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged LCK is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of LCK transfected lysate using LCK monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LCK rabbit polyclonal antibody.)
WB (Western Blot) (Western Blot analysis of LCK expression in transfected 293T cell line by LCK monoclonal antibody. Lane 1: LCK transfected lysate (59.4kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (LCK monoclonal antibody Western Blot analysis of LCK expression in Jurkat.)
FCM (Flow Cytometry) (Flow cytometric analysis of Jurkat cells with NCK1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining NCK1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining NCK1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining NCK1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NCK1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NCK1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-NCK1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-NCK1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of NCK1 on different lysates using anti-NCK1 antibody at 1/500 dilution. Positive control: Lane 1: Hela Lane 1: NIH-3T3 Lane 2: Rat kidney tissue Lane 3: Mouse testis tissue)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with VAMP8 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining VAMP8 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining VAMP8 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining VAMP8 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-VAMP8 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-VAMP8 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-VAMP8 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of VAMP8 on different lysates using anti-VAMP8 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: Mouse kidney)
IP (Immunoprecipitation) (Immunoprecipitation analysis of 300ug extracts of Jurkat cells using 3ug Vimentin antibody . Western blot was performed from the immunoprecipitate using Vimentin antibody at a dilition of 1:1000.)
IF (Immunofluorescence) (Confocal Immunofluorescent analysis of U2OS cells using KRR1 Rabbit pAb (A4487) at dilution of 1:100 (40x lens)(red). Vimentin (: Vimentin Rabbit mAb) for Cytoskeleton staining(green), DAPI for nuclear staining(blue).)
IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using Vimentin antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using Vimentin antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using Vimentin antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Mouse kidney using Vimentin antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human colon carcinoma using Vimentin antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat spleen using Vimentin antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts from normal (control) and Vimentin knockout (KO) 293T cells, using Vimentin antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using Vimentin antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)
WB (Western Blot) (Western Blot analysis of ACY1 expression in transfected 293T cell line by ACY1 monoclonal antibody. Lane 1: ACY1 transfected lysate (45.9kD). Lane 2: Non-transfected lysate.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ACY1 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ACY1 on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B tissue. [antibody concentration 3ug/ml])
IP (Immunoprecipitation) (Immunoprecipitation of ACY1 transfected lysate using ACY1 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with ACY1 rabbit polyclonal antibody.)
WB (Western Blot) (ACY1 monoclonal antibody Western Blot analysis of ACY1 expression in K-562.)
WB (Western Blot) (Western Blot detection against Immunogen (70.62kD).)
Application Data (Detection limit for recombinant GST tagged NODAL is ~0.3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NODAL on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NODAL on formalin-fixed paraffin-embedded human endometrium cancer. [antibody concentration 3ug/ml])
WB (Western Blot) (NODAL monoclonal antibody Western Blot analysis of NODAL expression in PC-12.)
WB (Western Blot) (NODAL monoclonal antibody Western Blot analysis of NODAL expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (33.66kD).)
Application Data (Detection limit for recombinant GST tagged MEIS2 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MEIS2 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to MEIS2 on formalin-fixed paraffin-embedded human spleen tissue. [antibody concentration 1ug/ml])
WB (Western Blot) (Western Blot analysis of MEIS2 expression in transfected 293T cell line by MEIS2 monoclonal antibody. Lane 1: MEIS2 transfected lysate (51.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (MEIS2 monoclonal antibody Western Blot analysis of MEIS2 expression in K-562.)
WB (Western Blot) (MEIS2 monoclonal antibody Western Blot analysis of MEIS2 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (67.65kD).)
WB (Western Blot) (MAP3K7IP1 monoclonal antibody Western Blot analysis of MAP3K7IP1 expression in PC-12.)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between HSPA1L and MAP3K7IP1 HeLa cells were stained with HSPA1L rabbit purified polyclonal 1:1200 and MAP3K7IP1 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Application Data (Detection limit for recombinant GST tagged MAP3K7IP1 is ~0.3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MAP3K7IP1 on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (MAP3K7IP1 monoclonal antibody Western Blot analysis of MAP3K7IP1 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (36.89kD).)
TAB1 (TGF-beta-activated Kinase 1 and MAP3K7-binding Protein 1, Mitogen-activated Protein Kinase Kinase Kinase 7-interacting Protein 1, TGF-beta-activated Kinase 1-binding Protein 1, TAK1-binding Protein 1, MAP3K7IP1) (Biotin)
Application Data (Detection limit for recombinant GST tagged SNRPA is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SNRPA on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SNRPA on formalin-fixed paraffin-embedded human heart tissue. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of SNRPA expression in transfected 293T cell line by SNRPA monoclonal antibody. Lane 1: SNRPA transfected lysate (31.3kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (SNRPA monoclonal antibody, Western Blot analysis of SNRPA expression in Hela.)
WB (Western Blot) (Western Blot detection against Immunogen (57.13kD).)
WB (Western Blot) (TRIM33 monoclonal antibody, Western Blot analysis of TRIM33 expression in Hela NE)
WB (Western Blot) (TRIM33 monoclonal antibody. Western Blot analysis of TRIM33 expression in 293)
Application Data (Detection limit for recombinant GST tagged TRIM33 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TRIM33 on HeLa cell. [antibody concentration 30ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TRIM33 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml])
WB (Western Blot) (TRIM33 monoclonal antibody. Western Blot analysis of TRIM33 expression in PC-12)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
IHC (Immunohistochemistry) (Figure 8. IHC analysis of Aconitase 2 using anti-Aconitase 2 antibody (AAA19706).Aconitase 2 was detected in a paraffin-embedded section of rat heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Aconitase 2 Antibody (AAA19706) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)
IHC (Immunohistochemistry) (Figure 7. IHC analysis of Aconitase 2 using anti-Aconitase 2 antibody (AAA19706).Aconitase 2 was detected in a paraffin-embedded section of mouse heart tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Aconitase 2 Antibody (AAA19706) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)
IHC (Immunohistchemistry) (Figure 6. IHC analysis of Aconitase 2 using anti-Aconitase 2 antibody (AAA19706).Aconitase 2 was detected in a paraffin-embedded section of human testicular germ cell tumor tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Aconitase 2 Antibody (AAA19706) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)
IHC (Immunohistochemistry) (Figure 5. IHC analysis of Aconitase 2 using anti-Aconitase 2 antibody (AAA19706).Aconitase 2 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Aconitase 2 Antibody (AAA19706) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)
IHC (Immunohistochemistry) (Figure 4. IHC analysis of Aconitase 2 using anti-Aconitase 2 antibody (AAA19706).Aconitase 2 was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Aconitase 2 Antibody (AAA19706) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)
IHC (Immunohistochemistry) (Figure 3. IHC analysis of Aconitase 2 using anti-Aconitase 2 antibody (AAA19706).Aconitase 2 was detected in a paraffin-embedded section of human hepatocellular carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Aconitase 2 Antibody (AAA19706) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)
IHC (Immunohistochemistry) (Figure 2. IHC analysis of Aconitase 2 using anti-Aconitase 2 antibody (AAA19706).Aconitase 2 was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Aconitase 2 Antibody (AAA19706) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)
WB (Western Blot) (Figure 1. Western blot analysis of Aconitase 2 using anti-Aconitase 2 antibody (AAA19706).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human U-87MG whole cell lysates,Lane 3: human Jurkat whole cell lysates,Lane 4: human SH-SY5Y whole cell lysates,Lane 5: rat skeletal muscle tissue lysates,Lane 6: rat brain tissue lysates,Lane 7: mouse skeletal muscle tissue lysates,Lane 8: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Aconitase 2 antigen affinity purified monoclonal antibody (#AAA19706) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Aconitase 2 at approximately 85 kDa. The expected band size for Aconitase 2 is at 85 kDa.)
Application Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti MouseCD19 antibody, clone 6D5, green in A and Rat anti Mlouse CD8 antibody, clone YTS105.18 , red in B. Merged image in C with nuclei counterstained blue using DAPI. Low power)
Application Data (Staining of mouse spleen cells with Rat anti Mouse CD19:RPE-Alexa Fluor 750 (AAA11931P750))
Application Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti MouseCD19 antibody, clone 6D5, green in A and Rat anti Mlouse CD8 antibody, clone YTS105.18 , red in B. Merged image in C with nuclei counterstained blue using DAPI. High power)
Application Data (Immunoperoxidase staining of a murine lymph node with Rat anti Mouse CD19 antibody, clone 6D5 forllowerd by horseradish peroxidase conjugated Goat anti Rat IgG . Low power)
Application Data (Staining of mouse spleen cells with Rat anti Mouse CD19)
Application Data (Immunoperoxidase staining of a murine lymph node with Rat anti Mouse CD19 antibody, clone 6D5 forllowerd by horseradish peroxidase conjugated Goat anti Rat IgG . High power)
Application Data (Staining of mouse spleen cells with Rat anti Mouse CD19:APC)
Application Data (Staining of mouse spleen cells with Rat anti Mouse CD19:FITC)
Application Data (Staining of mouse spleen cells with Rat anti Mouse CD19:RPE-Alexa Fluor 647 (AAA11931P647))
Application Data (Staining of mouse spleen cells with Rat anti Mouse CD19:RPE)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SP1 on HeLa cell. [antibody concentration 20 ug/ml])
WB (Western Blot) (SP1 monoclonal antibody (M03), clone 4C8 Western Blot analysis of SP1 expression in Hela S3 NE (Cat # L013V3).)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human smooth muscle. [antibody concentration 3 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human smooth muscle. [antibody concentration 3 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml])
Western Blot, Immunohistochemistry, Flow Cytometry
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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