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Application Data (Detection limit for recombinant GST tagged GCH1 is ~0.3ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of GCH1 transfected lysate using 127227and Protein A Magnetic Bead and immunoblotted with GCH1 rabbit polyclonal antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase on formalin-fixed paraffin-embedded human lymph node using 127227 (3ug/ml).)
WB (Western Blot) (Western Blot analysis of GCH1 expression in transfected 293T cell line using 127227. Lane 1: GCH1 transfected lysate (27.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot analysis of GCH1 using 127227 expression in IMR-32.)
WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)
WB (Western Blot) (Western Blot analysis of Mouse, Rat Brain showing detection of 14 kDa Alpha Synuclein protein using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 3C11. Lane 1: Molecular Weight Ladder (MW). Lane 2: Mouse brain cell lysate. Lane 3: Rat brain cell lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 14 kDa. Other Band(s): ~30 kDa (dimer).)
WB (Western Blot) (Western Blot analysis of Human Brain showing detection of 14 kDa Alpha Synuclein protein using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 3C11. Lane 1: Molecular Weight Ladder (MW). Lane 2: Parkinson brain cell lystate. Lane 3: Human brain cell lysate. Load: 15 ug. Block: 5% Skim Milk in 1X TBST. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Goat Anti-Mouse HRP:IgG at 1:3000 for 1 hour at RT. Color Development: ECL solution (Super Signal West Pico) for 5 min in RT. Predicted/Observed Size: 14 kDa. Other Band(s): 100 kDa (oligomer).)
ICC (Immunocytochemistry) (Immunocytochemistry/Immunofluorescence analysis using Mouse Anti-Alpha Synuclein Monoclonal Antibody, Clone 3C11. Tissue: Neuroblastoma cell line (SK-N-BE). Species: Human. Fixation: 4% Formaldehyde for 15 min at RT. Primary Antibody: Mouse Anti-Alpha Synuclein Monoclonal Antibody at 1:100 for 60 min at RT. Secondary Antibody: Goat Anti-Mouse ATTO 488 at 1:200 for 60 min at RT. Counterstain: Phalloidin Texas Red F-Actin stain; DAPI (blue) nuclear stain at 1:1000, 1:5000 for 60 min at RT, 5 min at RT. Localization: Cytoplasm: weak; Nucleus: Med. Magnification: 60X. (A) DAPI (blue) nuclear stain. (B) Phalloidin Texas Red F-Actin stain. (C) Alpha Synuclein Antibody. (D) Composite.)
WB (Western Blot) (LDB3 monoclonal antibody Western Blot analysis of LDB3 expression in A-431.)
WB (Western Blot) (Western blot analysis of LDB3 over-expressed 293 cell line, cotransfected with LDB3 Validated Chimera RNAi ((Lane 2) or non-transfected control (Lane 1). Blot probed with LDB3 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged LDB3 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to LDB3 on A-431 cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of LDB3 expression in transfected 293T cell line by LDB3 monoclonal antibody. Lane 1: LDB3 transfected lysate (31kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (57.24kD).)
WB (Western Blot) (Western Blot Analysis of Human Spleen cell lysate using HLA-Pan Mouse Monoclonal Antibody (CR3/43).)
WB (Western Blot) (Western Blot Analysis of Ramos cell lysate using HLA-Pan Mouse Monoclonal Antibody (CR3/43).)
SDS-PAGE (SDS-PAGE Analysis Purified HLA-Pan Mouse Monoclonal Antibody (CR3/43). Confirmation of Purity and Integrity of Antibody.)
IF (Immunofluorescence) (Immunofluorescent staining of Raji cells. HLA- Pan Mouse Monoclonal Antibody (CR3/43) followed by goat anti-Mouse IgG-CF488 (Green). The nuclear counterstain is Reddot (Red))
FCM (Flow Cytometry) (Flow Cytometric Analysis of Human Raji cells using HLA-Pan Mouse Monoclonal Antibody (CR3/43) followed by goat anti-Mouse IgG-CF488 (Blue); Isotype control (Red).)
IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Tonsil stained with HLA- Pan Mouse Monoclonal Antibody (CR3/43).)
IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Tonsil stained with HLA- Pan Mouse Monoclonal Antibody (CR3/43).)
Application Data (Detection limit for recombinant GST tagged UBE2C is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to UBE2C on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to UBE2C on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of UBE2C expression in transfected 293T cell line by UBE2C monoclonal antibody. Lane 1: UBE2C transfected lysate (19.7kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (UBE2C monoclonal antibody, Western Blot analysis of UBE2C expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (38.21kD).)
WB (Western Blot) (Western blot analysis of GPR3 over-expressed 293 cell line, cotransfected with GPR3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with GPR3 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged GPR3 is ~0.03ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GPR3 on formalin-fixed paraffin-embedded human transitional cell carcinoma tissue. [antibody concentration 1ug/ml].)
WB (Western Blot) (Western Blot analysis of GPR3 expression in transfected 293T cell line by GPR3 monoclonal antibody. Lane 1: GPR3 transfected lysate (35kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (GPR3 monoclonal antibody, Western Blot analysis of GPR3 expression in Jurkat.)
WB (Western Blot) (Western Blot detection against Immunogen (62.04kD).)
ICC (Immunocytochemistry) (ICC staining ACTL6A in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining ACTL6A in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ACTL6A antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-ACTL6A antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ACTL6A antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of ACTL6A on Jurkat cell (1) and mouse brain tissue (2) lysate using anti-ACTL6A antibody at 1/500 dilution.)
FCM (Flow Cytometry) (Flow cytometric analysis of PC-12 cells with HMGB1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody)
ICC (Immunocytochemistry) (ICC staining HMGB1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining HMGB1 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-HMGB1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-HMGB1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-HMGB1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HMGB1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of HMGB1 on different cell lysates using anti-HMGB1 antibody at 1/1, 000 dilution. Positive control: Lane 1: MCF-7 Lane 2: PC12 Lane 3: F9)
ICC (Immunocytochemistry) (ICC staining Cdk2 in N2A cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Cdk2 in SH-SY-5Y cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Cdk2 in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Cdk2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-Cdk2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Cdk2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cdk2 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Cdk2 on different cell lysates using anti-Cdk2 antibody at 1/1, 000 dilution. Positive control: Lane 1: A549 Lane 2: F9)
Application Data (Detection limit for recombinant GST tagged NFKB1 is approximately 3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NFKB1 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NFKB1 on HeLa cell. [antibody concentration 10 ug/ml])
WB (Western Blot) (NFKB1 monoclonal antibody (M03), clone 3F6 Western Blot analysis of NFKB1 expression in Hela S3 NE.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NFKB1 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to NFKB1 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml])
Application Data (Detection limit for recombinant GST tagged NFKB1 is approximately 3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NFKB1 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NFKB1 on HeLa cell. [antibody concentration 10 ug/ml])
WB (Western Blot) (NFKB1 monoclonal antibody (M03), clone 3F6 Western Blot analysis of NFKB1 expression in Hela S3 NE (Cat # L013V3).)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NFKB1 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to NFKB1 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml])
WB (Western Blot) (SMAD1 monoclonal antibody Western Blot analysis of SMAD1 expression in A-549.)
WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)
Application Data (Detection limit for recombinant GST tagged SMAD1 is ~3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SMAD1 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SMAD1 on formalin-fixed paraffin-embedded human salivary gland tissue. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of SMAD1 expression in transfected 293T cell line by SMAD1 monoclonal antibody. Lane 1: SMAD1 transfected lysate (52.3kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (SMAD1 monoclonal antibody. Western Blot analysis of SMAD1 expression in MES-SA/Dx5.)
WB (Western Blot) (PRPF19 monoclonal antibody. Western Blot analysis of PRPF19 expression in NIH/3T3.)
Application Data (Detection limit for recombinant GST tagged PRPF19 is 0.3ng/ml as a capture antibody.)
WB (Western Blot) (Western Blot analysis of PRPF19 expression in transfected 293T cell line by PRPF19 monoclonal antibody. Lane 1: PRPF19 transfected lysate (55.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (PRPF19 monoclonal antibody. Western Blot analysis of PRPF19 expression in Hela NE.)
WB (Western Blot) (PRPF19 monoclonal antibody. Western Blot analysis of PRPF19 expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (36.01kD).)
WB (Western Blot) (SMAD4 monoclonal antibody. Western Blot analysis of SMAD4 expression in IMR-32.)
WB (Western Blot) (Western Blot detection against Immunogen (86.46kD).)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between CDKN1A and SMAD4 HeLa cells were stained with CDKN1A rabbit purified polyclonal 1:1200 and SMAD4 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Application Data (Detection limit for recombinant GST tagged SMAD4 is ~3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SMAD4 on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (SMAD4 monoclonal antibody Western Blot analysis of SMAD4 expression in Hela NE.)
WB (Western Blot) (Western blot analysis of FOXA2 over-expressed 293 cell line, cotransfected with FOXA2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with FOXA2 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged FOXA2 is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of FOXA2 transfected lysate using FOXA2 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with FOXA2 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA2 on HepG2 cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of FOXA2 expression in transfected 293T cell line by FOXA2 monoclonal antibody Lane 1: FOXA2 transfected lysate (48.3kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (FOXA2 monoclonal antibody Western Blot analysis of FOXA2 expression in HepG2.)
WB (Western Blot) (Western Blot detection against Immunogen (36.19kD).)
Application Data (Proximity Ligation Analysis of protein-protein interactions between STAT1 and PDGFRB Mahlavu cells were stained with anti-STAT1 rabbit purified polyclonal (1:1200) and (1:50). Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
Application Data (Detection limit for recombinant GST tagged PDGFRB is ~1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of PDGFRB transfected lysate using and Protein A Magnetic Bead, and immunoblotted with PDGFRB rabbit polyclonal antibody.)
WB (Western Blot) (Western Blot analysis of PDGFRB expression in human uterus myoma using.)
WB (Western Blot) (Western Blot analysis of PDGFRB expression in human stomach using.)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
WB (Western Blot) (LETM1 monoclonal antibody Western Blot analysis of LETM1 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (37.62kD).)
Application Data (Detection limit for recombinant GST tagged LETM1 is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of LETM1 transfected lysate using LETM1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LETM1 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to LETM1 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to LETM1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of LETM1 expression in transfected 293T cell line by LETM1 monoclonal antibody.Lane 1: LETM1 transfected lysate (83.4kD).Lane 2: Non-transfected lysate.)
WB (Western Blot) (TXNDC4 monoclonal antibody Western Blot analysis of TXNDC4 expression in K-562.)
WB (Western Blot) (TXNDC4 monoclonal antibody Western Blot analysis of TXNDC4 expression in A-431)
Application Data (Detection limit for recombinant GST tagged TXNDC4 is 1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of TXNDC4 transfected lysate using TXNDC4 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with TXNDC4 rabbit polyclonal antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TXNDC4 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of TXNDC4 expression in transfected 293T cell line by TXNDC4 monoclonal antibody Lane 1: TXNDC4 transfected lysate (47kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (67.58kD).)
Thioredoxin Domain-containing Protein 4 (TXNDC4, Endoplasmic Reticulum Resident Protein 44, ER Protein 44, ERp44, KIAA0573, PDIA10, UNQ532/PRO1075) (FITC)
Application Data (Detection limit for recombinant GST tagged PGR is approximately 0.1ng/ml as a capture antibody.)
WB (Western Blot) (PGR monoclonal antibody (M04), clone 2C2 Western Blot analysis of PGR expression in A-431 (Cat # L015V1).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PGR on A-431 cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PGR on A-431 cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PGR on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 1.5 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to PGR on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 1.5 ug/ml])
WB (Western Blot) (Western blot analysis of NFIC over-expressed 293 cell line, cotransfected with NFIC Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NFIC monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NFIC on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NFIC on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of NFIC expression in transfected 293T cell line by NFIC monoclonal antibody. Lane 1: NFIC transfected lysate (47.6kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (NFIC monoclonal antibody Western Blot analysis of NFIC expression in A-431.)
WB (Western Blot) (Western Blot detection against Immunogen (72.82kD).)
WB (Western Blot) (Western Blot detection against Immunogen (37.44kD).)
WB (Western Blot) (CCT7 monoclonal antibody Western Blot analysis of CCT7 expression in human pancreas.)
Application Data (Detection limit for recombinant GST tagged CCT7 is ~0.3ng/ml as a capture antibody.)
WB (Western Blot) (Western Blot analysis of CCT7 expression in transfected 293T cell line by CCT7 monoclonal antibody. Lane 1: CCT7 transfected lysate (59.4kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (CCT7 monoclonal antibody Western Blot analysis of CCT7 expression in K-562.)
WB (Western Blot) (CCT7 monoclonal antibody Western Blot analysis of CCT7 expression in Raw 264.7)
WB (Western Blot) (CCT7 monoclonal antibody Western Blot analysis of CCT7 expression in HL-60)
WB (Western Blot) (Western blot analysis of MRPL12 over-expressed 293 cell line, cotransfected with MRPL12 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MRPL12 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged MRPL12 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MRPL12 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to MRPL12 on formalin-fixed paraffin-embedded human breast cancer tissue. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of MRPL12 expression in transfected 293T cell line by MRPL12 monoclonal antibody. Lane 1: MRPL12 transfected lysate (21.3kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (MRPL12 monoclonal antibody, Western Blot analysis of MRPL12 expression in COLO 320 HSR.)
IP (Immunoprecipitation) (Figure 6. Immunoprecipitation analysis. Cellular overexpression lysates (made from HEK293F cells transfected with DYKDDDDK tagged human BCMA full length gene) were pre-incubated with 6 different rabbit DimAb clones and negative control IgG. The immunocomplexes were further pulled down by protein A beads, fractionated, and blotted with mouse anti-DYKDDDDK monoclonal antibody.)
FCM (Flow Cytometry) (Figure 6. Immunoprecipitation analysis. Cellular overexpression lysates (made from HEK293F cells transfected with DYKDDDDK tagged human BCMA full length gene) were pre-incubated with 6 different rabbit DimAb clones and negative control IgG. The immunocomplexes were further pulled down by protein A beads, fractionated, and blotted with mouse anti-DYKDDDDK monoclonal antibody.)
FCM (Flow Cytometry) (Figure 6. Immunoprecipitation analysis. Cellular overexpression lysates (made from HEK293F cells transfected with DYKDDDDK tagged human BCMA full length gene) were pre-incubated with 6 different rabbit DimAb clones and negative control IgG. The immunocomplexes were further pulled down by protein A beads, fractionated, and blotted with mouse anti-DYKDDDDK monoclonal antibody.)
Application Data (Figure 6. Immunoprecipitation analysis. Cellular overexpression lysates (made from HEK293F cells transfected with DYKDDDDK tagged human BCMA full length gene) were pre-incubated with 6 different rabbit DimAb clones and negative control IgG. The immunocomplexes were further pulled down by protein A beads, fractionated, and blotted with mouse anti-DYKDDDDK monoclonal antibody.)
Application Data (Figure 6. Immunoprecipitation analysis. Cellular overexpression lysates (made from HEK293F cells transfected with DYKDDDDK tagged human BCMA full length gene) were pre-incubated with 6 different rabbit DimAb clones and negative control IgG. The immunocomplexes were further pulled down by protein A beads, fractionated, and blotted with mouse anti-DYKDDDDK monoclonal antibody.)
Application Data (Figure 6. Immunoprecipitation analysis. Cellular overexpression lysates (made from HEK293F cells transfected with DYKDDDDK tagged human BCMA full length gene) were pre-incubated with 6 different rabbit DimAb clones and negative control IgG. The immunocomplexes were further pulled down by protein A beads, fractionated, and blotted with mouse anti-DYKDDDDK monoclonal antibody.)
Application Data (Detection limit for recombinant GST tagged TYK2 is approximately 1ng/ml as a capture antibody.)
WB (Western Blot) (TYK2 monoclonal antibody (M03), clone 6H1 Western Blot analysis of TYK2 expression in Hela S3 NE.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TYK2 on formalin-fixed paraffin-embedded human lymphoma. [antibody concentration 3 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TYK2 on formalin-fixed paraffin-embedded human lymphoma. [antibody concentration 3 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TYK2 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to TYK2 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml])
FCM (Flow Cytometry) (Flow cytometric analysis of MCF-7 cells with Fragilis antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining Fragilis in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Fragilis in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Fragilis in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Fragilis antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Fragilis antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Fragilis on different cell lysates using anti-Fragilis antibody at 1/500 dilution. Positive control: Lane 1: Hela Lane 2: HepG2 Lane 3: Hela)
WB (Western Blot) (Western Blot analysis of AFP expression in transfected 293T cell line by AFP monoclonal antibody. Lane 1: AFP transfected lysate (69kD). Lane 2: Non-transfected lysate.)
Application Data (Detection limit for recombinant GST tagged AFP is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of AFP transfected lysate using AFP monoclonal antibody and Protein A Magnetic Bead and immunoblotted with AFP rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to AFP on HepG2 cell. [antibody concentration 30ug/ml].)
WB (Western Blot) (AFP monoclonal antibody Western Blot analysis of AFP expression in HepG2.)
WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)
WB (Western Blot) (Western Blot analysis of B2M expression in transfected 293T cell line by B2M monoclonal antibody. Lane 1: B2M transfected lysate (13.7kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (B2M monoclonal antibody, Western Blot analysis of B2M expression in U-2 OS.)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between CALR and B2M. HeLa cells were stained with CALR rabbit purified polyclonal 1:1200 and B2M mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Application Data (Detection limit for recombinant GST tagged B2M is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to B2M on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to B2M on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western blot analysis of B2M over-expressed 293 cell line, cotransfected with B2M Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with B2M monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
WB (Western Blot) (Western Blot analysis of GABPA expression in transfected 293T cell line by GABPA monoclonal antibody (M03), clone M1.Lane 1: GABPA transfected lysate (Predicted MW: 51.3 KDa).Lane 2: Non-transfected lysate.)
IP (Immunoprecipitation) (Immunoprecipitation of GABPA transfected lysate using anti-GABPA monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with GABPA monoclonal antibody.)
Application Data (Detection limit for recombinant GST tagged GABPA is approximately 1ng/ml as a capture antibody.)
WB (Western Blot) (GABPA monoclonal antibody (M03), clone M1 Western Blot analysis of GABPA expression in Hela S3 NE.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GABPA on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GABPA on HeLa cell. [antibody concentration 10 ug/ml])
Application Data (Staining of KG1 lymphocytes with Mouse anti Human CD59:FITC)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD59: Azide Free)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD59:RPE)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD59:Alexa Fluor488)
Application Data (Published customer image: The effect of protease treatment on influenza virion associated host proteins. Purified influenza A/WSN/33 virus was either mock treated or subjected to overnight digestion with subtilisin followed by concentration through a sucrose cushion. 10 ug of mock infected cell lysate (lane 1) or influenza infected cell lysate (lane 2) and 2 ug of untreated influenza virions (lane 3) or protease treated influenza virions (lane 4) were then analyzed by western blot with antibodies against the indicated proteins. Numbers to the right are molecular weight markers.From: Shaw ML, Stone KL, Colangelo CM, Gulcicek EE, Palese P (2008) Cellular Proteins in Influenza Virus Particles. PLoS Pathog 4(6): e1000085.)
Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD59:Alexa Fluor 647)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD59)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD59)
Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD59:Biotin)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b)
Application Data (Staining of mouse peritoneal macrophages cells with Rat anti Mouse CD11b:Alexa Fluor 488)
Application Data (Staining of total mouse peritoneal exudate cells demonstrating labelling of macrophages with Rat anti Mouse CD11b:RPE)
Application Data (Published customer image: Immunocytochemical characterisation of cultures of mouse microglia. Cultures of microglia were immunostained with anti-CD11b (a) and anti-GFAP (c). Cultures of astrocytes were immunostained with anti-CD11b (c) and anti-GFAP (d). All cells were also counterstained with DAPI (blue) to identify the cells' nuclei.From: Ferger et al. Journal of Neuroinflammation 2010 7:45)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b:RPE)
Application Data (Staining of mouse peritoneal macrophages cells with Rat anti Mouse CD11b:FITC)
Application Data (Immunoperoxidase staining of mouse lymph node cryosection using Rat anti Mouse CD11b antibody followed by horseradish peroxidase conjugated Goat anti Rat IgG . High power)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b: Alexa Fluor 647)
Application Data (Immunoperoxidase staining of mouse lymph node cryosection using Rat anti Mouse CD11b antibody followed by horseradish peroxidase conjugated Goat anti Rat IgG . Medium power)
WB (Western Blot) (Western blot analysis of UGP2 over-expressed 293 cell line, cotransfected with UGP2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with UGP2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged UGP2 is ~0.1ng/ml as a capture antibody.)
WB (Western Blot) (Western Blot analysis of UGP2 expression in transfected 293T cell line by UGP2 monoclonal antibody (M01). Lane 1: UGP2 transfected lysate (56.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (UGP2 monoclonal antibody Western Blot analysis of UGP2 expression in NIH/3T3.)
WB (Western Blot) (UGP2 monoclonal antibody Western Blot analysis of UGP2 expression in Raw 264.7)
WB (Western Blot) (UGP2 monoclonal antibody Western Blot analysis of UGP2 expression in PC-12)
WB (Western Blot) (UGP2 monoclonal antibody Western Blot analysis of UGP2 expression in HeLa)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ROD1 on HeLa cell. [antibody concentration 20ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ROD1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml])
WB (Western Blot) (ROD1 monoclonal antibody. Western Blot analysis of ROD1 expression in NIH/3T3)
WB (Western Blot) (ROD1 monoclonal antibody Western Blot analysis of ROD1 expression in Hela NE.)
WB (Western Blot) (ROD1 monoclonal antibody Western Blot analysis of ROD1 expression in PC-12)
WB (Western Blot) (Western Blot detection against Immunogen (36.63kD).)
Application Data (Detection limit for recombinant GST tagged YY1 is approximately 0.03ng/ml as a capture antibody.)
WB (Western Blot) (YY1 monoclonal antibody (M04), clone 2C5 Western Blot analysis of YY1 expression in Hela S3 NE.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to YY1 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to YY1 on HeLa cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to YY1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 7 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to YY1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 7 ug/ml])
Application Data (Detection limit for recombinant GST tagged TYK2 is approximately 1ng/ml as a capture antibody.)
WB (Western Blot) (TYK2 monoclonal antibody (M03), clone 6H1 Western Blot analysis of TYK2 expression in Hela S3 NE.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TYK2 on formalin-fixed paraffin-embedded human lymphoma. [antibody concentration 3 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TYK2 on formalin-fixed paraffin-embedded human lymphoma. [antibody concentration 3 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TYK2 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to TYK2 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml])
Application Data (Detection limit for recombinant GST tagged CSE1L is approximately 0.1ng/ml as a capture antibody.)
WB (Western Blot) (CSE1L monoclonal antibody (M04), clone 2F4 Western Blot analysis of CSE1L expression in Hela S3 NE (Cat # L013V3).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CSE1L on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CSE1L on HeLa cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CSE1L on formalin-fixed paraffin-embedded human testis. [antibody concentration 6 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to CSE1L on formalin-fixed paraffin-embedded human testis. [antibody concentration 6 ug/ml])
Application Data (Detection limit for recombinant GST tagged YY1 is approximately 0.03ng/ml as a capture antibody.)
WB (Western Blot) (YY1 monoclonal antibody (M02), clone 4A5 Western Blot analysis of YY1 expression in Hela S3 NE (Cat # L013V3).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to YY1 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to YY1 on HeLa cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to YY1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to YY1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml])
Application Data (Proximity Ligation Analysis of protein-protein interactions between PRKCZ and AKT3. HeLa cells were stained with anti-PRKCZ rabbit purified polyclonal 1:1200 and anti-AKT3 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
Application Data (Detection limit for recombinant GST tagged AKT3 is ~0.03ng/ml as a capture antibody.)
WB (Western Blot) (AKT3 monoclonal antibody Western Blot analysis of AKT3 expression in MCF-7.)
WB (Western Blot) (AKT3 monoclonal antibody Western Blot analysis of AKT3 expression in Raw 264.7.)
WB (Western Blot) (AKT3 monoclonal antibody Western Blot analysis of AKT3 expression in PC-12.)
WB (Western Blot) (AKT3 monoclonal antibody Western Blot analysis of AKT3 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)
WB (Western Blot) (Western blot analysis of ARID3A over-expressed 293 cell line, cotransfected with ARID3A Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ARID3A monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged ARID3A is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ARID3A on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ARID3A on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml].)
WB (Western Blot) (ARID3A monoclonal antibody, Western Blot analysis of ARID3A expression in K-562.)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
ARID3A (AT-rich Interactive Domain-containing Protein 3A, ARID Domain-containing Protein 3A, B Cell Regulator of IgH Transcription, Bright, Dead Ringer-like Protein 1, E2F-binding Protein 1, DRIL1, DRIL3, DRX, E2FBP1) (FITC)
WB (Western Blot) (Western Blot analysis of ALB expression in transfected 293T cell line by ALB monoclonal antibody. Lane 1: ALB transfected lysate (69.4kD). Lane 2: Non-transfected lysate.)
Application Data (Detection limit for recombinant GST tagged ALB is ~1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of ALB transfected lysate using ALB monoclonal antibody and Protein A Magnetic Bead and immunoblotted with ALB rabbit polyclonal antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ALB on formalin-fixed paraffin-embedded human hepatocellular carcinoma tissue. [antibody concentration 3ug/ml].)
WB (Western Blot) (ALB monoclonal antibody Western Blot analysis of ALB expression in HepG2.)
WB (Western Blot) (Western Blot detection against Immunogen (90.75kD).)
WB (Western Blot) (Western blot analysis of ARID3A over-expressed 293 cell line, cotransfected with ARID3A Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ARID3A monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged ARID3A is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ARID3A on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ARID3A on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml].)
WB (Western Blot) (ARID3A monoclonal antibody, Western Blot analysis of ARID3A expression in K-562.)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
ARID3A (AT-rich Interactive Domain-containing Protein 3A, ARID Domain-containing Protein 3A, B Cell Regulator of IgH Transcription, Bright, Dead Ringer-like Protein 1, E2F-binding Protein 1, DRIL1, DRIL3, DRX, E2FBP1) (HRP)
Application Data (Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 followed by horseradish peroxidase Goat anti Mouse IgG2a antibody as a detection reagent. Medium power)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD3:RPE)
Application Data (Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 followed by horseradish peroxidase Goat anti Mouse IgG2a antibody as a detection reagent. Low power)
Application Data (ELISA analysis of human CD3 expression using Rat anti Human CD3ε, clone CD3-12 as a capture reagent and biotinylated Mouse anti Human CD3, clone UCHT1 as a detection reagent with recombinant human CD3 as antigen to produce the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . A serum (green) sample is included undiluted)
Application Data (Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 followed by horseradish peroxidase Goat anti Mouse IgG2a antibody as a detection reagent. High power)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD3:Alexa Fluor 405)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD3)
Application Data (Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 followed by horseradish peroxidase Goat anti Mouse IgG2a antibody as a detection reagent. High power)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD3: RPE- Alexa Fluor 750)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD3: FITC)
WB (Western Blot) (Western Blot analysis of ATP6V1G2 expression in transfected 293T cell line by ATP6V1G2 monoclonal antibody. Lane 1: ATP6V1G2 transfected lysate (13.6kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (ATP6V1G2 monoclonal antibody Western Blot analysis of ATP6V1G2 expression in NIH/3T3.)
WB (Western Blot) (ATP6V1G2 monoclonal antibody Western Blot analysis of ATP6V1G2 expression in Raw 264.7.)
WB (Western Blot) (ATP6V1G2 monoclonal antibody, Western Blot analysis of ATP6V1G2 expression in HepG2.)
WB (Western Blot) (ATP6V1G2 monoclonal antibody. Western Blot analysis of ATP6V1G2 expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (34.32kD).)
ATP6V1G2 (V-type Proton ATPase Subunit G 2, V-ATPase Subunit G 2, V-ATPase 13kD Subunit 2, Vacuolar Proton Pump Subunit G 2, ATP6G, ATP6G2, NG38) APC
Gene Names
ATP6V1G2; NG38; ATP6G; VMA10; ATP6G2
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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