Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
WB (Western Blot) (Detection of human CEACAM1 by western blot. Samples: Whole cell lysate (5 ug) from HEK293T, UACC-257, GaMG, Hep-G2, U2OS, A-549, SK-MEL-28, MCF-7, HT-29, and Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CEACAM1 recombinant monoclonal antibody (AAA23829 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
IP (Immunoprecipitation) (Detection of human CEACAM1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from UACC-257 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CEACAM1 recombinant monoclonal antibody (AAA23829 lot 1) used for IP at 6 ul per reaction. CEACAM1 was also immunoprecipitated by rabbit anti-CEACAM1/5 recombinant monoclonal antibody . For blotting immunoprecipitated CEACAM1, AAA23829 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry) (Detection of human CEACAM1 in FFPE liver carcinoma by IHC. Antibody: Rabbit anti-CEACAM1 recombinant monoclonal antibody (AAA23829 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry) (Detection of human CEACAM1 in FFPE breast carcinoma by IHC. Antibody: Rabbit anti-CEACAM1 recombinant monoclonal antibody (AAA23829 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry) (Detection of human CEACAM1 in FFPE Hep-G2 cells by ICC. Antibody: Rabbit anti-CEACAM1 recombinant monoclonal antibody (AAA23829 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
FCM (Flow Cytometry) (Detection of human CEACAM1 (shaded) in HT-29 cells by flow cytometry. Antibody: Rabbit anti-CEACAM1 recombinant monoclonal antibody (AAA23829) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
WB (Western Blot) (Western Blot detection against Immunogen (85.84kD).)
WB (Western Blot) (Western Blot detection against Immunogen (85.84kD).)
Application Data (Detection limit for recombinant GST tagged STIP1 is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of STIP1 transfected lysate using STIP1 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with STIP1 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to STIP1 on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to STIP1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 0.3ug/ml].)
WB (Western Blot) (Western Blot analysis of STIP1 expression in transfected 293T cell line by STIP1 monoclonal antibody Lane 1: STIP1 transfected lysate (62.6kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (LZTFL1 monoclonal antibody, Western Blot analysis of LZTFL1 expression in HeLa.)
Application Data (Detection limit for recombinant GST tagged LZTFL1 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoDetection limit for recombinant GST tagged LZTFL1 is ~0.1ng/ml as a capture antibody.clonal antibody to LZTFL1 on HeLa cell. [antibody concentration 25ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to LZTFL1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of LZTFL1 expression in transfected 293T cell line by LZTFL1 monoclonal antibody. Lane 1: LZTFL1 transfected lysate (34.6kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
FCM (Flow Cytometry) (Flow cytometric analysis of SH-SY-5Y cells with PSD95 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining PSD95 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining PSD95 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse cerebullum tissue using anti-PSD95 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-PSD95 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PSD95 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of PSD95 on different lysates using anti-PSD95 antibody at 1/1, 000 dilution. Positive control: Lane 1: Mouse brain Lane 2: Human brain)
FCM (Flow Cytometry) (Flow cytometric analysis of LOVO cells with Wnt2b antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining Wnt2b in SHSY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Wnt2b in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Wnt2b in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Wnt2b antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Wnt2b antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Wnt2b antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Wnt2b on SiHa cell lysates using anti-Wnt2b at 1/500 dilution.)
ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-DDB2 mAb. [Lot No. 2246C4a-1])
WB (Western Blot) (Detection of human DDB2 by Western blot.Samples: Whole cell lysate (25 ug) from HeLa cells. [Lot No. 2246C4a-1]Predicted molecular weight: 47 kDa)
WB (Western Blot) (ADPGK monoclonal antibody. Western Blot analysis of ADPGK expression in Raw 264.7.)
WB (Western Blot) (ADPGK monoclonal antibody, Western Blot analysis of ADPGK expression in HeLa.)
WB (Western Blot) (Western blot analysis of ADPGK over-expressed 293 cell line, cotransfected with ADPGK Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ADPGK monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged ADPGK is 0.3ng/ml as a capture antibody.)
WB (Western Blot) (Western Blot analysis of ADPGK expression in transfected 293T cell line by ADPGK monoclonal antibody. Lane 1: ADPGK transfected lysate (54.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (ADPGK monoclonal antibody. Western Blot analysis of ADPGK expression in NIH/3T3.)
WB (Western Blot) (Western Blot detection against Immunogen (34.03kD).)
WB (Western Blot) (Western blot analysis of SARS over-expressed 293 cell line, cotransfected with SARS Validated Chimera RNAi (301-R01V) (Lane 2) or non-transfected control (Lane 1). Blot probed with SARS monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged SARS is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SARS on HeLa cell. [antibody concentration 25ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SARS on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of SARS expression in transfected 293T cell line by SARS monoclonal antibody. Lane 1: SARS transfected lysate (58.8kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (SARS monoclonal antibody, Western Blot analysis of SARS expression in Jurkat.)
WB (Western Blot) (Western Blot detection against Immunogen (82.28kD).)
WB (Western Blot) (PGRMC2 monoclonal antibody Western Blot analysis of PGRMC2 expression in PC-12)
WB (Western Blot) (PGRMC2 monoclonal antibody Western Blot analysis of PGRMC2 expression in Raw 264.7)
Application Data (Detection limit for recombinant GST tagged PGRMC2 is ~0.3ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PGRMC2 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1ug/ml])
WB (Western Blot) (PGRMC2 monoclonal antibody Western Blot analysis of PGRMC2 expression in NIH/3T3)
WB (Western Blot) (PGRMC2 monoclonal antibody Western Blot analysis of PGRMC2 expression in HeLa)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
WB (Western Blot) (LZTFL1 monoclonal antibody, Western Blot analysis of LZTFL1 expression in HeLa.)
Application Data (Detection limit for recombinant GST tagged LZTFL1 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoDetection limit for recombinant GST tagged LZTFL1 is ~0.1ng/ml as a capture antibody.clonal antibody to LZTFL1 on HeLa cell. [antibody concentration 25ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to LZTFL1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of LZTFL1 expression in transfected 293T cell line by LZTFL1 monoclonal antibody. Lane 1: LZTFL1 transfected lysate (34.6kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
Application Data (Detection limit for recombinant GST tagged GCH1 is ~0.3ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of GCH1 transfected lysate using 127227and Protein A Magnetic Bead and immunoblotted with GCH1 rabbit polyclonal antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase on formalin-fixed paraffin-embedded human lymph node using 127227 (3ug/ml).)
WB (Western Blot) (Western Blot analysis of GCH1 expression in transfected 293T cell line using 127227. Lane 1: GCH1 transfected lysate (27.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot analysis of GCH1 using 127227 expression in IMR-32.)
WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)
FCM (Flow Cytometry) (Flow cytometric analysis of HepG2 cells with SULT2A1 antibody at 1/100 dilution (yellow) compared with an unlabelled control (cells without incubation with primary antibody; purple).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining SULT2A1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining SULT2A1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-SULT2A1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-SULT2A1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-SULT2A1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-SULT2A1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat adrenal gland tissue using anti-SULT2A1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of SULT2A1 on mouse kidney tissue lysate using anti-SULT2A1 antibody at 1/1, 000 dilution.)
WB (Western Blot) (BUB1B monoclonal antibody Western Blot analysis of BUB1B expression in Hela NE.)
WB (Western Blot) (Western Blot detection against Immunogen (39.93kD).)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between CDC20 and BUB1B. HeLa cells were stained with CDC20 rabbit purified polyclonal 1:1200 and BUB1B mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
WB (Western Blot) (Western blot analysis of BUB1B over-expressed 293 cell line, cotransfected with BUB1B Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with BUB1B monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged BUB1B is ~0.03ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to BUB1B on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of BUB1B expression in transfected 293T cell line by BUB1B monoclonal antibody. Lane 1: BUB1B transfected lysate (119.5kD). Lane 2: Non-transfected lysate.)
FCM (Flow Cytometry) (Flow cytometric analysis of Daudi cells with SENP1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining SENP1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining SENP1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining SENP1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human small Intestine tissue using anti-SENP1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-SENP1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of SENP1 on K562 cell using anti-SENP1 antibody at 1/1, 000 dilution.)
Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD86 antibody, clone 42F followed by horseradish peroxidase conjugated Goat anti Mouse IgG . Low power)
Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD86 antibody, clone 42F followed by horseradish peroxidase conjugated Goat anti Mouse IgG . Medium power)
Application Data (Staining of rat splenocytes with Mouse anti Rat CD86:RPE)
Application Data (Staining of rat splenocytes with Mouse anti Rat CD86:Alexa Fluor ?647)
Application Data (Staining of rat splenocytes with Mouse anti Rat CD86:Low Endotoxin)
Application Data (Staining of rat splenocytes with Mouse anti Rat CD86:FITC)
Application Data (Staining of rat splenocytes with Mouse anti Rat CD86:Alexa Fluor ?488)
Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD86 antibody, clone 42F followed by horseradish peroxidase conjugated Goat anti Mouse IgG . High power)
ICC (Immunocytochemistry) (ICC staining ERG in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining ERG in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-ERG antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-ERG antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ERG antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of ERG on Jurkat cell lysates using anti-ERG antibody at 1/1, 000 dilution.)
FCM (Flow Cytometry) (Flow cytometric analysis of MCF-7 cells with eIF4A3 antibody at 1/50 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining eIF4A3 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining eIF4A3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining eIF4A3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-eIF4A3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-eIF4A3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-eIF4A3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-eIF4A3 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of eIF4A3 on SiHa cell lysates using anti-eIF4A3 at 1/500 dilution.)
WB (Western Blot) (CLK3 monoclonal antibody. Western Blot analysis of CLK3 expression in Hela NE.)
WB (Western Blot) (Western blot analysis of CLK3 over-expressed 293 cell line, cotransfected with CLK3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CLK3 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CLK3 on HeLa cell. [antibody concentration 8ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CLK3 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.5ug/ml].)
WB (Western Blot) (Western Blot analysis of CLK3 expression in transfected 293T cell line by CLK3 monoclonal antibody.Lane 1: CLK3 transfected lysate (58.6kD).Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
WB (Western Blot) (Western blot analysis of PCDH8 over-expressed 293 cell line, cotransfected with PCDH8 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PCDH8 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged PCDH8 is ~0.1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PCDH8 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of PCDH8 expression in transfected 293T cell line by PCDH8 monoclonal antibody. Lane 1: PCDH8 transfected lysate (113kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (PCDH8 monoclonal antibody Western Blot analysis of PCDH8 expression in COLO 320 HSR.)
WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)
WB (Western Blot) (SMAD1 monoclonal antibody Western Blot analysis of SMAD1 expression in A-549.)
WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)
Application Data (Detection limit for recombinant GST tagged SMAD1 is ~3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SMAD1 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SMAD1 on formalin-fixed paraffin-embedded human salivary gland tissue. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of SMAD1 expression in transfected 293T cell line by SMAD1 monoclonal antibody. Lane 1: SMAD1 transfected lysate (52.3kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (SMAD1 monoclonal antibody. Western Blot analysis of SMAD1 expression in MES-SA/Dx5.)
WB (Western Blot) (Western blot analysis of PITX1 over-expressed 293 cell line, cotransfected with PITX1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PITX1 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged PITX1 is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of PITX1 transfected lysate using PITX1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PITX1 rabbit polyclonal antibody.)
WB (Western Blot) (Western Blot analysis of PITX1 expression in transfected 293T cell line by PITX1 monoclonal antibody Lane 1: PITX1 transfected lysate (34.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (PITX1 monoclonal antibody Western Blot analysis of PITX1 expression in HeLa NE.)
WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)
WB (Western Blot) (PKNOX2 monoclonal antibody. Western Blot analysis of PKNOX2 expression in NIH/3T3.)
WB (Western Blot) (Western blot analysis of PKNOX2 over-expressed 293 cell line, cotransfected with PKNOX2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PKNOX2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PKNOX2 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of PKNOX2 expression in transfected 293T cell line by PKNOX2 monoclonal antibody. Lane 1: PKNOX2 transfected lysate (51.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (PKNOX2 monoclonal antibody, Western Blot analysis of PKNOX2 expression in Hela NE.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
Application Data (Detection limit for recombinant GST tagged RPS5 is ~3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RPS5 on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of RPS5 expression in transfected 293T cell line by RPS5 monoclonal antibody. Lane 1: RPS5 transfected lysate (22.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (RPS5 monoclonal antibody. Western Blot analysis of RPS5 expression in NIH/3T3.)
WB (Western Blot) (RPS5 monoclonal antibody. Western Blot analysis of RPS5 expression in Raw 264.7.)
WB (Western Blot) (RPS5 monoclonal antibody. Western Blot analysis of RPS5 expression in PC-12.)
WB (Western Blot) (RPS5 monoclonal antibody, Western Blot analysis of RPS5 expression in HeLa.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TIMP2 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TIMP2 on HeLa cell. [antibody concentration 10 ug/ml])
Application Data (Detection limit for recombinant GST tagged TIMP2 is approximately 0.3ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TIMP2 on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TIMP2 on formalin-fixed paraffin-embedded human spleen. [antibody concentration 3 ug/ml])
WB (Western Blot) (TIMP2 monoclonal antibody (M04), clone 5B11 Western Blot analysis of TIMP2 expression in HeLa (Cat # L013V1).)
Application Data (Detection limit for recombinant GST tagged TCF7L2 is approximately 0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TCF7L2 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TCF7L2 on HeLa cell. [antibody concentration 10 ug/ml])
WB (Western Blot) (TCF7L2 monoclonal antibody (M06), clone 3A4 Western Blot analysis of TCF7L2 expression in K-562.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TCF7L2 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to TCF7L2 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml])
Application Data (Detection limit for recombinant GST tagged YY1 is approximately 0.03ng/ml as a capture antibody.)
WB (Western Blot) (YY1 monoclonal antibody (M02), clone 4A5 Western Blot analysis of YY1 expression in Hela S3 NE (Cat # L013V3).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to YY1 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to YY1 on HeLa cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to YY1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to YY1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml])
FCM (Flow Cytometry) (Flow cytometric analysis of K562 cells with GCNF antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
ICC (Immunocytochemistry) (ICC staining GCNF (green) in HepG2 cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-GCNF antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human cerebellum tissue using anti-GCNF antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of GCNF on different cell lysates using anti-GCNF antibody at 1/1, 000 dilution. Positive control�� Lane1: K562 Lane2: NTERA-2 Lane3: HEK293 Lane4: HUVE-12 Lane5: HeLa)
WB (Western Blot) (Western blot analysis of GCNF on HEK293 (1) and GCNF -hIgGFc transfected HEK293 (2) cell lysate using anti-GCNF antibody at 1/1, 000 dilution.)
WB (Western Blot) (Western blot analysis of GCNF on human GCNF recombinant protein using anti- GCNF antibody at 1/1, 000 dilution.)
IF (Immunofluorescence) (Confocal immunofluorescent analysis of C2C12 cells, U0126-treated (left, M2865-5010 μM for 1 h) or TPA-treated (right, #T5600-03 200 nM for 15 min), using AAA14743 (green) Actin filaments have been labeled with Alexa Fluor® 555 phalloidin (red). Blue pseudocolor = DRAQ5™ (fluorescent DNA dye).)
FCM (Flow Cytometry) (Flow cytometric analysis of Jurkat cells, U0126-treated (blue) or PMA-treated (green), using AAA14743)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung carcinoma, untreated (left) or λ phosphatase-treated (right), using AAA14743)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma using AAA14743)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded NIH/3T3 cells, untreated (left) or PMA-treated (right), using AAA14743)
WB (Western Blot) (Western blot analysis of extracts from 293, NIH/3T3 and C6 cells, treated with λ phosphatase or TPA as indicated, using AAA14743 (upper), or M2360-02 (lower).)
WB (Western Blot) (CLK3 monoclonal antibody. Western Blot analysis of CLK3 expression in Hela NE.)
WB (Western Blot) (Western blot analysis of CLK3 over-expressed 293 cell line, cotransfected with CLK3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CLK3 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CLK3 on HeLa cell. [antibody concentration 8ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CLK3 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.5ug/ml].)
WB (Western Blot) (Western Blot analysis of CLK3 expression in transfected 293T cell line by CLK3 monoclonal antibody. Lane 1: CLK3 transfected lysate (58.6kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
Application Data (Detection limit for recombinant GST tagged CSE1L is approximately 0.1ng/ml as a capture antibody.)
WB (Western Blot) (CSE1L monoclonal antibody (M05), clone 3F8 Western Blot analysis of CSE1L expression in K-562.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CSE1L on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CSE1L on HeLa cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CSE1L on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to CSE1L on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml])
FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of HepG2 cells using anti-Neurofibromin/NF1 antibody (AAA19654).Overlay histogram showing HepG2 cells stained with AAA19654 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Neurofibromin/NF1 Antibody (AAA19654, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)
IHC (Immunohistchemistry) (Figure 6. IHC analysis of Neurofibromin/NF1 using anti-Neurofibromin/NF1 antibody (AAA19654).Neurofibromin/NF1 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Neurofibromin/NF1 Antibody (AAA19654) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)
IHC (Immunohistochemistry) (Figure 5. IHC analysis of Neurofibromin/NF1 using anti-Neurofibromin/NF1 antibody (AAA19654).Neurofibromin/NF1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Neurofibromin/NF1 Antibody (AAA19654) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)
IHC (Immunohistochemistry) (Figure 4. IHC analysis of Neurofibromin/NF1 using anti-Neurofibromin/NF1 antibody (AAA19654).Neurofibromin/NF1 was detected in a paraffin-embedded section of human metaplasia of squamous cells of the renal pelvis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Neurofibromin/NF1 Antibody (AAA19654) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)
IHC (Immunohistochemistry) (Figure 3. IHC analysis of Neurofibromin/NF1 using anti-Neurofibromin/NF1 antibody (AAA19654).Neurofibromin/NF1 was detected in a paraffin-embedded section of human bladder epithelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Neurofibromin/NF1 Antibody (AAA19654) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)
IHC (Immunohistochemistry) (Figure 2. IHC analysis of Neurofibromin/NF1 using anti-Neurofibromin/NF1 antibody (AAA19654).Neurofibromin/NF1 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Neurofibromin/NF1 Antibody (AAA19654) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)
WB (Western Blot) (Figure 1. Western blot analysis of Neurofibromin/NF1 using anti-Neurofibromin/NF1 antibody (AAA19654).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: rat brain tissue lysates,Lane 3: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Neurofibromin/NF1 antigen affinity purified monoclonal antibody (#AAA19654) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Neurofibromin/NF1 at approximately 319 kDa. The expected band size for Neurofibromin/NF1 is at 319 kDa.)
Application Data (Detection limit for recombinant GST tagged SORD is ~0.1ng/ml as a capture antibody.)
IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to SORD on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1.2ug/ml].)
WB (Western Blot) (Western Blot analysis of SORD expression in transfected 293T cell line by SORD monoclonal antibody. Lane 1: SORD transfected lysate (38.165kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (SORD monoclonal antibody. Western Blot analysis of SORD expression in Raw 264.7.)
WB (Western Blot) (SORD monoclonal antibody, Western Blot analysis of SORD expression in Hela NE.)
WB (Western Blot) (SORD monoclonal antibody. Western Blot analysis of SORD expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (38.21kD).)
WB (Western Blot) (Western blot analysis of CITED1 over-expressed 293 cell line, cotransfected with CITED1 Validated Chimera RNAi ((Lane 2) or non-transfected control (Lane 1). Blot probed with CITED1 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged CITED1 is ~0.3ng/ml as a capture antibody.)
WB (Western Blot) (Western Blot analysis of CITED1 expression in transfected 293T cell line by CITED1 monoclonal antibody. Lane 1: CITED1 transfected lysate (19.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (CITED1 monoclonal antibody Western Blot analysis of CITED1 expression in A-431.)
WB (Western Blot) (CITED1 monoclonal antibody. Western Blot analysis of CITED1 expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
WB (Western Blot) (Western blot analysis of PITX1 over-expressed 293 cell line, cotransfected with PITX1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PITX1 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged PITX1 is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of PITX1 transfected lysate using PITX1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PITX1 rabbit polyclonal antibody.)
WB (Western Blot) (Western Blot analysis of PITX1 expression in transfected 293T cell line by PITX1 monoclonal antibody Lane 1: PITX1 transfected lysate (34.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (PITX1 monoclonal antibody Western Blot analysis of PITX1 expression in HeLa NE.)
WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)
WB (Western Blot) (SAV1 monoclonal antibody (M02), Western Blot analysis of SAV1 expression in Hela NE.)
Application Data (Detection limit for recombinant GST tagged SAV1 is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of SAV1 transfected lysate using SAV1 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SAV1 on HeLa cell. [antibody concentration 60ug/ml].)
WB (Western Blot) (SAV1 monoclonal antibody. Western Blot analysis of SAV1 expression in HepG2.)
WB (Western Blot) (Western Blot detection against Immunogen (35.35kD).)
WB (Western Blot) (Western Blot analysis of NDUFV2 expression in transfected 293T cell line by NDUFV2 monoclonal antibody. Lane 1: NDUFV2 transfected lysate (Predicted MW: 27.4kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (NDUFV2 monoclonal antibody Western Blot analysis of NDUFV2 expression in K-562.)
WB (Western Blot) (NDUFV2 monoclonal antibody Western Blot analysis of NDUFV2 expression in NIH/3T3.)
WB (Western Blot) (NDUFV2 monoclonal antibody Western Blot analysis of NDUFV2 expression in Raw 264.7.)
WB (Western Blot) (NDUFV2 monoclonal antibody Western Blot analysis of NDUFV2 expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
Application Data (Detection limit for recombinant GST tagged GLUD2 is 0.3 ng/ml as a capture antibody.)
WB (Western Blot) (GLUD2 monoclonal antibody (M01), clone 3C2. Western Blot analysis of GLUD2 expression in human pancreas.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GLUD2 on formalin-fixed paraffin-embedded human ovarian cancer. [antibody concentration 1 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GLUD2 on formalin-fixed paraffin-embedded human ovarian cancer. [antibody concentration 1 ug/ml])
IP (Immunoprecipitation) (Immunoprecipitation of GLUD2 transfected lysate using anti-GLUD2 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with GLUD2 MaxPab rabbit polyclonal antibody.)
WB (Western Blot) (Western Blot analysis of GLUD2 expression in transfected 293T cell line by GLUD2 monoclonal antibody (M01), clone 3C2.Lane 1: GLUD2 transfected lysate (61 KDa).Lane 2: Non-transfected lysate.)
IHC (Immunohistochemistry) (IHC image diluted at 1:1000 and staining in paraffin-embedded human kidney tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistochemistry) (IHC image diluted at 1:1000 and staining in paraffin-embedded human lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistchemistry) (IHC image diluted at 1:1000 and staining in paraffin-embedded human lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistochemistry) (IHC image diluted at 1:1000 and staining in paraffin-embedded human lung cancer tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistochemistry) (IHC image diluted at 1:1000 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistchemistry) (IHC image diluted at 1:1000 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistochemistry) (IHC image diluted at 1:1000 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
WB (Western Blot) (Western Blot Positive WB detected in: MCF-7 whole cell lysate All lanes: PKM antibody at 1:4000, 1:8000, 1:16000, 1:32000, 1:64000, 1:128000, 1:256000, 1:512000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 58 kDa Observed band size: 58 KDa Exposure time: 5min)
WB (Western Blot) (Western Blot Positive WB detected in: MCF-7 whole cell lysate at 40ug, 20ug, 10ug, 5ug, 2.5ug, 1.25ug, 0.625ug All lanes: PKM antibody at 1:1000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 58 kDa Observed band size: 58 KDa Exposure time: 5min)
WB (Western Blot) (Western Blot Positive WB detected in: Mouse Brain tissue, Mouse Skeldtal Muscle tissue All lanes: PKM antibody at 1:1000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 58 kDa Observed band size: 58 KDa Exposure time: 5min)
WB (Western Blot) (Western Blot Positive WB detected in: Hela whole cell lysate, MCF-7 whole cell lysate, Jurkat whole cell lysate All lanes: PKM antibody at 1:1000 Secondary Goat polyclonal to Mouse IgG at 1/10000 dilution Predicted band size: 58 kDa Observed band size: 58 KDa Exposure time: 1min)
Western Blot, Immunohistochemistry, Immunofluorescence, Flow Cytometry, Immunoprecipitation
Purity
>95%, Protein G purified
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
Submit a Question
Please complete the form below and a representative will contact you as soon as possible.
Request more Information
Please complete the form below and a representative will contact you as soon as possible.
Request a Manual
Please complete the form below and a representative will contact you as soon as possible.
Request a Quote
Please complete the form below and a representative will contact you as soon as possible.