Monoclonal Antibodies

Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.

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ICC (Immunocytochemistry) (ICC staining GART in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining GART in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining GART in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-GART antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-GART antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of GART on different lysates using anti-GART antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: 293T Lane 3: A431)

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GART, Monoclonal Antibody (Cat# AAA30137)

Full Name

GART Antibody

Gene Names

GART; AIRS; GARS; PAIS; PGFT; PRGS; GARTF

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Cortactin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining Cortactin in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Cortactin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Cortactin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Cortactin antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Cortactin antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Cortactin antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Cortactin on different lysates using anti-Cortactin antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3 Lane 3: A431)

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Cortactin, Monoclonal Antibody (Cat# AAA30148)

Full Name

Cortactin Antibody

Gene Names

CTTN; EMS1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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Application Data

Application Data

Application Data

Application Data

Application Data

SDS-PAGE (Whole cell lysates of A549 were separated by 12% SDS-PAGE, and the membrane was blotted with anti-p21(PTR2559) antibody. The HRP-conjugated Goat anti-M)

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p21, Monoclonal Antibody (Cat# AAA30851)

Full Name

p21 (PTR2559) Antibody

Gene Names

CDKN1A; P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot, Immunofluorescence

Purity

Protein G

Pricing

$240/0.02 mL $315/0.05 mL $375/0.1 mL $600/0.2 mL $1,140/2x0.2 mL $1,650/3x0.2 mL $2,135/4x0.2 mL $2,350/5x0.2 mL

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IHC (Immunohistochemistry) (Dilution: WB: 1:1000 IHC: 1:200 IF 1:200)

WB (Western Blot) (Dilution: WB: 1:1000 IHC: 1:200 IF 1:200)

IF (Immunofluorescence) (Dilution: WB: 1:1000 IHC: 1:200 IF 1:200)

IF (Immunofluorescence) (Dilution: WB: 1:1000 IHC: 1:200 IF 1:200)

IHC (Immunohistochemistry-Paraffin) (Dilution: WB: 1:1000 IHC: 1:200 IF 1:200)

IHC (Immunohistochemistry-Paraffin) (Dilution: WB: 1:1000 IHC: 1:200 IF 1:200)

WB (Western Blot) (Dilution: WB: 1:1000 IHC: 1:200 IF 1:200)

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CDX2, Monoclonal Antibody (Cat# AAA28864)

Full Name

CDX2 Monoclonal Antibody (14H6)

Gene Names

CDX2; CDX3; CDX-3

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged CDH11 is 3 ng/ml as a capture antibody.)

Application Data (Detection limit for recombinant GST tagged CDH11 is 1 ng/ml as a capture antibody.)

WB (Western Blot) (CDH11 monoclonal antibody (M05), clone 3C8. Western Blot analysis of CDH11 expression in human kidney.)

WB (Western Blot) (CDH11 monoclonal antibody (M05), clone 3C8. Western Blot analysis of CDH11 expression in MCF-7.)

Application Data (PC3-MM2 cells were stained with CDH11-FITC labeled monoclonal antibody (Green). The cell nucleus were counterstained with DAPI (Blue).)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CDH11 on PC3-MM2 cell. [antibody concentration 10 ug/ml])

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CDH11, Monoclonal Antibody (Cat# AAA26411)

Full Name

CDH11 (Cadherin 11, Type 2, OB-Cadherin (Osteoblast), CAD11, CDHOB, OB, OSF-4) (FITC)

Gene Names

CDH11; OB; CAD11; CDHOB; OSF-4

Applications

Immunofluorescence, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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SDS-PAGE (Mouse lung whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-TTF1(ABT237) antibody. The HRP-conjugated Goat anti-M)

IHC (Immunohistochemistry) (Human thyroid tissue was stained with Anti-TTF1 (ABT237) Antibody)

IHC (Immunohistchemistry) (Human thyroid tissue was stained with Anti-TTF1 (ABT237) Antibody)

IHC (Immunohistochemistry) (Human thyroid papillary carcinoma tissue was stained with Anti-TTF1 (ABT237) Antibody)

IHC (Immunohistochemistry) (Human thyroid papillary carcinoma tissue was stained with Anti-TTF1 (ABT237) Antibody)

IHC (Immunohistchemistry) (Human lung tissue was stained with Anti-TTF1 (ABT237) Antibody)

IHC (Immunohistochemistry) (Human lung tissue was stained with Anti-TTF1 (ABT237) Antibody)

IHC (Immunohistochemistry) (Human lung squamous cell carcinoma tissue was stained with Anti-TTF1 (ABT237) Antibody)

IHC (Immmunohistochemistry) (Human lung squamous cell carcinoma tissue was stained with Anti-TTF1 (ABT237) Antibody)

IHC (Immunohistochemistry) (Human lung adenocarcinoma tissue was stained with Anti-TTF1 (ABT237) Antibody)

IHC (Immunohistochemistry) (Human lung adenocarcinoma tissue was stained with Anti-TTF1 (ABT237) Antibody)

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TTF1, Monoclonal Antibody (Cat# AAA30840)

Full Name

TTF1 (ABT237) Antibody

Gene Names

NKX2-1; BCH; BHC; NK-2; TEBP; TTF1; NKX2A; T/EBP; TITF1; TTF-1; NKX2.1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Purity

The antibody was affinity-purified from ascites by affinity-chromatography using specific immunogen.

Pricing

$325/0.04 mL $445/0.1 mL $685/0.2 mL $1,310/2x0.2 mL $1,905/3x0.2 mL $2,460/4x0.2 mL $2,760/5x0.2 mL

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of THP-1 cells using anti-Ku70 antibody (AAA19362).Overlay histogram showing THP-1 cells stained with AAA19362 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti- Ku70 Antibody (AAA19362, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-mouse IgG (BA1126, 5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 8. IF analysis of Ku70 using anti- Ku70 antibody (AAA19362).Ku70 was detected in immunocytochemical section of U20S cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL mouse anti- Ku70 Antibody (AAA19362) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Mouse IgG (BA1126) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of Ku70 using anti-Ku70 antibody (AAA19362).Ku70 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Ku70 Antibody (AAA19362) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of Ku70 using anti-Ku70 antibody (AAA19362).Ku70 was detected in paraffin-embedded section of human gallbladder adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Ku70 Antibody (AAA19362) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of Ku70 using anti-Ku70 antibody (AAA19362).Ku70 was detected in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Ku70 Antibody (AAA19362) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of Ku70 using anti-Ku70 antibody (AAA19362).Ku70 was detected in paraffin-embedded section of human adrenocortical adenoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Ku70 Antibody (AAA19362) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of Ku70 using anti-Ku70 antibody (AAA19362).Ku70 was detected in paraffin-embedded section of human ovarian serous adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Ku70 Antibody (AAA19362) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of Ku70 using anti-Ku70 antibody (AAA19362).Ku70 was detected in paraffin-embedded section of human renal clear cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml mouse anti-Ku70 Antibody (AAA19362) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of Ku70 using anti-Ku70 antibody (AAA19362).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human Hela whole cell lysatesLane 2: human A549 whole cell lysatesLane 3: human HepG2 whole cell lysatesLane 4: human MCF-7 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with mouse anti- Ku70 antigen affinity purified monoclonal antibody (Catalog # AAA19362) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for Ku70 at approximately 70KD. The expected band size for Ku70 is at 70KD.)

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Ku70, Monoclonal Antibody (Cat# AAA19362)

Full Name

Anti-Ku70 Antibody (monoclonal, 3D7)

Gene Names

XRCC6; ML8; KU70; TLAA; CTC75; CTCBF; G22P1

Reactivity

Human

Applications

WB, IHC-P, ICC, IF, FC/FACS/FCM

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-DHX29 mAb. [Lot No. 2269C1-1])

FCM (Flow Cytometry) (HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-DHX29 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. 2269C1-1])

IP (Immunoprecipitation) (Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-DHX29 mAb. [Lot No. 2269C1-1]Predicted molecular weight: 155 kDa)

WB (Western Blot) (Detection of human DHX29 by Western blot.Samples: Whole cell lysate (25 ug) from HeLa cells. [Lot No. 2269C1-1]Predicted molecular weight: 155 kDa)

Quality Control (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Quality Control (Western blot analysis of immunized recombinant protein, using anti-DHX29 monoclonal antibody.)

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DHX29, Monoclonal Antibody (Cat# AAA10609)

Full Name

Mouse monoclonal antibody Anti-Human DHX29

Gene Names

DHX29; DDX29

Reactivity

Human

Applications

Dot Blot, Immunocytochemistry, Immunoprecipitation, Western Blot, Flow Cytometry

Pricing

$615/0.1 mg $1,170/2x0.1 mg $1,695/3x0.1 mg $2,190/4x0.1 mg $2,435/5x0.1 mg

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IHC (Immunohistochemistry) (Figure 8. IHC analysis of FABP4 using anti-FABP4 antibody (AAA19688).FABP4 was detected in a paraffin-embedded section of mouse intestines tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-FABP4 Antibody (AAA19688) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of FABP4 using anti-FABP4 antibody (AAA19688).FABP4 was detected in a paraffin-embedded section of mouse intestines tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-FABP4 Antibody (AAA19688) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of FABP4 using anti-FABP4 antibody (AAA19688).FABP4 was detected in a paraffin-embedded section of rat intestines tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-FABP4 Antibody (AAA19688) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of FABP4 using anti-FABP4 antibody (AAA19688).FABP4 was detected in a paraffin-embedded section of human lymphadenoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-FABP4 Antibody (AAA19688) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of FABP4 using anti-FABP4 antibody (AAA19688).FABP4 was detected in a paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-FABP4 Antibody (AAA19688) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of FABP4 using anti-FABP4 antibody (AAA19688).FABP4 was detected in a paraffin-embedded section of human gall bladder adenosquamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-FABP4 Antibody (AAA19688) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of FABP4 using anti-FABP4 antibody (AAA19688).FABP4 was detected in a paraffin-embedded section of human renal clear cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-FABP4 Antibody (AAA19688) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of FABP4 using anti-FABP4 antibody (AAA19688).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human RT4 whole cell lysates,Lane 2: human HL-60 whole cell lysates,Lane 3: rat thymus tissue lysates,Lane 4: mouse thymus tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-FABP4 antigen affinity purified monoclonal antibody (#AAA19688) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for FABP4 at approximately 15 kDa. The expected band size for FABP4 is at 15 kDa.)

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FABP4, Monoclonal Antibody (Cat# AAA19688)

Full Name

Anti-FABP4 Antibody Picoband (monoclonal, 10E12)

Gene Names

FABP4; aP2; ALBP; AFABP; A-FABP; HEL-S-104

Reactivity

Human, Mouse, Rat

Applications

WB, IHC

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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WB (Western Blot) (Detection of human CD28 by western blot. Samples: Whole cell lysate (10 ug) from HEK293T, Jurkat, HeLa, RPMI-8226, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD28 recombinant monoclonal antibody (AAA23865 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)

WB (Western Blot) (Detection of mouse CD28 by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, EL4, CH27, RAW 264.7, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD28 recombinant monoclonal antibody (AAA23865 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)

IP (Immunoprecipitation) (Detection of human CD28 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from Jurkat cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD28 recombinant monoclonal antibody (AAA23865 lot 1) used for IP at 6 ul/mg lysate. CD28 was also immunoprecipitated by a second antibody against a different epitope of CD28 . For blotting immunoprecipitated CD28, AAA23865 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)

IHC (Immunohistochemistry) (Detection of human CD28 by immunohistochemistry. Sample: FFPE section of tonsil. Antibody: Rabbit anti-CD28 recombinant monoclonal antibody (AAA23865 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

IHC (Immunohistochemistry) (Detection of mouse CD28 by immunohistochemistry. Sample: FFPE section of mouse gut. Antibody: Rabbit anti-CD28 recombinant monoclonal antibody (AAA23865 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

ICC (Immunocytochemistry) (Detection of human CD28 by immunocytochemistry. Sample: FFPE section of jurkat cells. Antibody: Rabbit anti-CD28 recombinant monoclonal antibody (AAA23865 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

ICC (Immunocytochemistry) (Detection of mouse CD28 by immunocytochemistry. Sample: FFPE section of EL4 cells. Antibody: Rabbit anti-CD28 recombinant monoclonal antibody (AAA23865 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

FCM (Flow Cytometry) (Detection of human CD28 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-CD28 recombinant monoclonal antibody (AAA23865) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)

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CD28, Monoclonal Recombinant Antibody (Cat# AAA23865)

Full Name

Rabbit anti-CD28 Recombinant Monoclonal Antibody [BLR200J]

Gene Names

CD28; Tp44; MGC138290

Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry

Purity

Purified

Pricing

$275/0.01 mL $680/0.1 mL $925/5x0.01 mL $1,300/2x0.1 mL $1,890/3x0.1 mL $2,440/4x0.1 mL $2,755/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged SRPK1 is ~0.03ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SRPK1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1ug/ml].)

WB (Western Blot) (Western Blot analysis of SRPK1 expression in transfected 293T cell line by SRPK1 monoclonal antibody. Lane 1: SRPK1 transfected lysate (74.3kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (SRPK1 monoclonal antibody, Western Blot analysis of SRPK1 expression in IMR-32.)

WB (Western Blot) (SRPK1 monoclonal antibody. Western Blot analysis of SRPK1 expression in HepG2.)

WB (Western Blot) (Western Blot detection against Immunogen (37kD).)

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SRPK1, Monoclonal Antibody (Cat# AAA24967)

Full Name

SRPK1 (SRSF Protein Kinase 1, SFRS Protein Kinase 1, Serine/Arginine-rich Protein-specific Kinase 1, SR-protein-specific Kinase 1) (Biotin)

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IP (Immunoprecipitation) (Immunoprecipitation of PSMA7 transfected lysate using PSMA7 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PSMA7 rabbit polyclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PSMA7 on HeLa cell. [antibody concentration 1 ~ 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PSMA7 on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 5ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PSMA7 on formalin-fixed paraffin-embedded human lung, adenosqumous cell carcinoma. [antibody concentration 5ug/ml])

WB (Western Blot) (Western Blot analysis of PSMA7 expression in transfected 293T cell line by PSMA7 monoclonal antibody. Lane 1: PSMA7 transfected lysate (Predicted MW: 27.9kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (PSMA7 monoclonal antibody Western Blot analysis of PSMA7 expression in HeLa.)

WB (Western Blot) (PSMA7 monoclonal antibody. Western Blot analysis of PSMA7 expression in human omentum, serous carcinoma.)

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PSMA7, Monoclonal Antibody (Cat# AAA24921)

Full Name

PSMA7 (Proteasome Subunit alpha Type-7, Proteasome Subunit RC6-1, Proteasome Subunit XAPC7, HSPC, MGC3755) (Biotin)

Gene Names

PSMA7; C6; HSPC; RC6-1; XAPC7; HEL-S-276

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (ACTB monoclonal antibody (M03), clone 1E7. Western Blot analysis of ACTB expression in Raw 264.7.)

WB (Western Blot) (ACTB monoclonal antibody (M03), clone 1E7. Western Blot analysis of ACTB expression in PC-12.)

WB (Western Blot) (ACTB monoclonal antibody (M03), clone 1E7. Western Blot analysis of ACTB expression in NIH/3T3.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ACTB on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ACTB on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml])

WB (Western Blot) (ACTB monoclonal antibody (M03), clone 1E7. Western Blot analysis of ACTB expression in Jurkat.)

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ACTB, Monoclonal Antibody (Cat# AAA26125)

Full Name

ACTB (Actin, beta, PS1TP5BP1) (APC)

Gene Names

ACTB; BRWS1; PS1TP5BP1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (NME2 monoclonal antibody (M06), clone 1D3. Western Blot analysis of NME2 expression in NIH/3T3.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NME2 on formalin-fixed paraffin-embedded human testis. [antibody concentration 1.5 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NME2 on formalin-fixed paraffin-embedded human testis. [antibody concentration 1.5 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NME2 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NME2 on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (NME2 monoclonal antibody (M06), clone 1D3 Western Blot analysis of NME2 expression in HeLa.)

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NME2, Monoclonal Antibody (Cat# AAA26206)

Full Name

NME2 (Non-Metastatic Cells 2, Protein (NM23B) Expressed in, MGC111212, NDPK-B, NDPKB, NM23-H2, NM23B, puf) (Biotin)

Gene Names

NME2; PUF; NDKB; NDPKB; NM23B; NDPK-B; NM23-H2

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SP1 on HeLa cell. [antibody concentration 20 ug/ml])

WB (Western Blot) (SP1 monoclonal antibody (M03), clone 4C8 Western Blot analysis of SP1 expression in Hela S3 NE.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human smooth muscle. [antibody concentration 3 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human smooth muscle. [antibody concentration 3 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3 ug/ml])

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SP1, Monoclonal Antibody (Cat# AAA25903)

Full Name

SP1 (Sp1 Transcription Factor) (AP)

Applications

Immunohistochemistry, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (RPS6KB1 monoclonal antibody (M04), clone 4H4. Western Blot analysis of RPS6KB1 expression in PC-12.)

WB (Western Blot) (RPS6KB1 monoclonal antibody (M04), clone 4H4. Western Blot analysis of RPS6KB1 expression in NIH/3T3.)

Application Data (Detection limit for recombinant GST tagged RPS6KB1 is approximately 0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RPS6KB1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RPS6KB1 on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (RPS6KB1 monoclonal antibody (M04), clone 4H4 Western Blot analysis of RPS6KB1 expression in HeLa (Cat # L013V1).)

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RPS6KB1, Monoclonal Antibody (Cat# AAA26371)

Full Name

RPS6KB1 (Ribosomal Protein S6 Kinase, 70kD, Polypeptide 1, PS6K, S6K, S6K1, STK14A, p70(S6K)-alpha, p70-S6K, p70-alpha) (FITC)

Gene Names

RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha

Applications

Immunofluorescence, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Zyxin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining Zyxin in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Zyxin in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse smooth muscle tissue using anti-Zyxin antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Zyxin antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Zyxin on MCF-7 cell using anti-Zyxin antibody at 1/500 dilution.)

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Zyxin, Monoclonal Antibody (Cat# AAA30399)

Full Name

Zyxin Antibody

Gene Names

ZYX; ESP-2; HED-2

Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry, Immunoprecipitation

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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IHC (Immunohistochemistry) (LAG3 Antibody-Immunocytochemistry of LAG-3 in over expressing HEK293 cells using LAG-3 antibody and control mouse IgG antibody (left corner box) at 1 ug/ml.)

IHC (Immunohistchemistry) (LAG3 Antibody-Human Spleen: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (LAG3 Antibody-Human Thymus: Formalin-Fixed, Paraffin-Embedded (FFPE))

IHC (Immunohistochemistry) (LAG3 Antibody-Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE))

ICC (Immunocytochemistry) (LAG3 Antibody-Immunofluorescence of LAG-3 in over human spleen tissue using LAG-3 Antibody at 10 ug/ml. Red: LAG3 Antibody [9F9] Blue: DAPI staining)

ICC (Immunocytochemistry) (LAG3 Antibody-Immunofluorescence of LAG-3 in over expressing HEK293 cells using LAG-3 Antibody at 2 ug/ml. Green: LAG3 Antibody [9F9] Blue: DAPI staining)

FCM (Flow Cytometry) (LAG3 Antibody-Flow cytometry analysis of LAG-3 over expressing HEK293 cells using LAG-3 antibody at 1 ug/ml. Blue: untransfected HEK293 cells. Yellow: LAG-3 over expressing HEK293 cells.)

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LAG3, Monoclonal Antibody (Cat# AAA21352)

Full Name

LAG3 Mouse anti-Human Monoclonal (9F9) Antibody

Gene Names

LAG3; CD223

Reactivity

Human

Applications

Flow Cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry

Purity

Protein A purified

Pricing

$690/0.05 mg $1,325/2x0.05 mg $1,915/3x0.05 mg $2,475/4x0.05 mg $2,800/5x0.05 mg

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WB (Western Blot) (NEK10 monoclonal antibody, Western Blot analysis of NEK10 expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (36.01kD).)

Application Data (Detection limit for recombinant GST tagged NEK10 is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NEK10 on HeLa cell. [antibody concentration 10ug/ml].)

WB (Western Blot) (Western Blot analysis of NEK10 expression in transfected 293T cell line by NEK10 monoclonal antibody.Lane 1: NEK10 transfected lysate (53.1kD).Lane 2: Non-transfected lysate.)

WB (Western Blot) (NEK10 monoclonal antibody. Western Blot analysis of NEK10 expression in NIH/3T3.)

WB (Western Blot) (NEK10 monoclonal antibody. Western Blot analysis of NEK10 expression in Raw 264.7.)

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NEK10, Monoclonal Antibody (Cat# AAA24068)

Full Name

NEK10 (Never in Mitosis A-related Kinase 10, NimA-related Protein Kinase 10, FLJ32685, Serine/Threonine Protein Kinase Nek10, FLJ32685)

Reactivity

Human, Mouse

Applications

Western Blot, Immunofluorescence

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

$820/0.1 mg $1,565/2x0.1 mg $2,270/3x0.1 mg $2,940/4x0.1 mg $3,380/5x0.1 mg

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WB (Western Blot) (F3 monoclonal antibody, Western Blot analysis of F3 expression in A-431.)

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between F7 and F3. HeLa cells were stained with F7 rabbit purified polyclonal 1:1200 and F3 mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex)

Application Data (Detection limit for recombinant GST tagged F3 is ~0.03ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of F3 transfected lysate using F3 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with F3 rabbit polyclonal antibody.)

WB (Western Blot) (Western Blot analysis of F3 expression in transfected 293T cell line by F3 monoclonal antibody. Lane 1: F3 transfected lysate (33.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)

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CD142, Monoclonal Antibody (Cat# AAA24160)

Full Name

CD142 (CD142 Antigen, Coagulation Factor III, F3, Tissue Factor, TF, TFA, Thromboplastin) (AP)

Gene Names

F3; TF; TFA; CD142

Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of LYPLA3 expression in NIH/3T3)

WB (Western Blot) (Western Blot analysis of PLA2G15 expression in transfected 293T cell line)

WB (Western Blot) (Western Blot analysis of LYPLA3 expression in Raw 264.7)

WB (Western Blot) (Western Blot analysis of LYPLA3 expression in PC-12)

WB (Western Blot) (Western Blot analysis of LYPLA3 expression in HeLa)

WB (Western Blot) (Western Blot detection against Immunogen using (37kD).)

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LYPLA3, Monoclonal Antibody (Cat# AAA24851)

Full Name

LYPLA3 (PLA2G15, Group XV Phospholipase A2, 1-O-acylceramide Synthase, ACS, LCAT-like Lysophospholipase, LLPL, Lysophospholipase 3, Lysosomal Phospholipase A2, LPLA2, UNQ341/PRO540) (Biotin)

Gene Names

PLA2G15; ACS; LLPL; LPLA2; LYPLA3; GXVPLA2

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western blot analysis of PDK2 over-expressed 293 cell line, cotransfected with PDK2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PDK2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged PDK2 is ~0.3ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PDK2 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 0.8ug/ml])

WB (Western Blot) (Western Blot analysis of PDK2 expression in transfected 293T cell line by PDK2 monoclonal antibody. Lane 1: PDK2 transfected lysate (46.2kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (PDK2 monoclonal antibody Western Blot analysis of PDK2 expression in U-2 OS.)

WB (Western Blot) (Western Blot detection against Immunogen (35.31kD).)

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PDHK2, Monoclonal Antibody (Cat# AAA24898)

Full Name

PDHK2 [Pyruvate Dehydrogenase [Lipoamide]] Kinase Isozyme 2, Mitochondrial, Pyruvate Dehydrogenase Kinase Isoform 2, PDH Kinase 2, PDKII, PDK2) (Biotin)

Gene Names

PDK2; PDHK2; PDKII

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (ACTB monoclonal antibody Western Blot analysis of ACTB expression in K-562.)

WB (Western Blot) (ACTB monoclonal antibody Western Blot analysis of ACTB expression in NIH/3T3.)

Application Data (Detection limit for recombinant GST tagged ACTB is ~1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ACTB on formalin-fixed paraffin-embedded human smooth muscle. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of ACTB expression in transfected 293T cell line by ACTB monoclonal antibody Lane 1: ACTB transfected lysate (42kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (ACTB monoclonal antibody Western Blot analysis of ACTB expression in Raw 264.7.)

WB (Western Blot) (ACTB monoclonal antibody Western Blot analysis of ACTB expression in HeLa.)

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ACTB, Monoclonal Antibody (Cat# AAA24416)

Full Name

ACTB (Actin, Cytoplasmic 1, Beta-actin) APC

Gene Names

ACTB; BRWS1; PS1TP5BP1

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GSTZ1 on HepG2 cell. [antibody concentration 10ug/ml].)

WB (Western Blot) (Western Blot analysis of GSTZ1 expression in transfected 293T cell line by GSTZ1 monoclonal antibody. Lane 1: GSTZ1 transfected lysate (24.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (GSTZ1 monoclonal antibody. Western Blot analysis of GSTZ1 expression in NIH/3T3.)

WB (Western Blot) (GSTZ1 monoclonal antibody, Western Blot analysis of GSTZ1 expression in HepG2.)

WB (Western Blot) (GSTZ1 monoclonal antibody. Western Blot analysis of GSTZ1 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (37.62kD).)

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GSTZ1, Monoclonal Antibody (Cat# AAA25708)

Full Name

GSTZ1 (Glutathione S-transferase zeta 1, Glutathione S-Transferase zeta 1, GSTZ1-1, Maleylacetoacetate Isomerase, MAAI, MAI, MGC2029) (PE)

Gene Names

GSTZ1; MAI; MAAI; MAAID; GSTZ1-1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between CASP3 and HCLS1. HeLa cells were stained with anti-CASP3 rabbit purified polyclonal 1:1200 and anti-HCLS1 mouse monoclonal antibody 1:50. Signals were detected by Duolink® 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

WB (Western Blot) (Western blot analysis of HCLS1 over-expressed 293 cell line, cotransfected with HCLS1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with HCLS1 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged HCLS1 is ~0.1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HCLS1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of HCLS1 expression in transfected 293T cell line by HCLS1 monoclonal antibody. Lane 1: HCLS1 transfected lysate (54kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (HCLS1 monoclonal antibody Western Blot analysis of HCLS1 expression in K-562.)

WB (Western Blot) (Western Blot detection against Immunogen (35.64kD).)

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HCLS1, Monoclonal Antibody (Cat# AAA25711)

Full Name

HCLS1 (Hematopoietic Lineage Cell-specific Protein, Hematopoietic Cell-specific LYN Substrate 1, LckBP1, p75, HS1) (PE)

Gene Names

HCLS1; HS1; p75; CTTNL; lckBP1

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western blot analysis of NDUFS4 over-expressed 293 cell line, cotransfected with NDUFS4 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NDUFS4 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged NDUFS4 is ~0.3ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NDUFS4 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1ug/ml])

WB (Western Blot) (Western Blot analysis of NDUFS4 expression in transfected 293T cell line by NDUFS4 monoclonal antibody. Lane 1: NDUFS4 transfected lysate (20.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (NDUFS4 monoclonal antibody Western Blot analysis of NDUFS4 expression in A-431.)

WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)

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NDUFS4, Monoclonal Antibody (Cat# AAA25467)

Full Name

NDUFS4 (NADH Dehydrogenase (Ubiquinone) Fe-S Protein 4, Mitochondrial, NADH-Ubiquinone Oxidoreductase 18kD Subunit, Complex I-18kD, CI-18kD, Complex I-AQDQ, CI-AQDQ) (HRP)

Gene Names

NDUFS4; AQDQ; CI-18; CI-AQDQ; CI-18 kDa

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged RUNX1 is approximately 1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RUNX1 on HeLa cell. [antibody concentration 40 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RUNX1 on HeLa cell. [antibody concentration 40 ug/ml])

WB (Western Blot) (RUNX1 monoclonal antibody (M06), clone 2C10 Western Blot analysis of RUNX1 expression in Hela S3 NE.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RUNX1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1.5 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to RUNX1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1.5 ug/ml])

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RUNX1, Monoclonal Antibody (Cat# AAA26215)

Full Name

RUNX1 (Runt-Related Transcription Factor 1, AML1, AML1-EVI-1, AMLCR1, CBFA2, EVI-1, PEBP2aB) (Biotin)

Gene Names

RUNX1; AML1; CBFA2; EVI-1; AMLCR1; PEBP2aB; AML1-EVI-1

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IHC (Immunohistchemistry) (Immunochemical staining CD3 in cynomolgus lymph node with rabbit monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry) (Immunochemical staining CD3 in cynomolgus spleen with rabbit monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)

FCM (Flow Cytometry) (Flow cytometric analysis of Human CD3E expression on human whole blood lymphocytes. Cells were stained with purified anti-Human CD3E, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of viable lymphocytes.)

IHC (Immunohistochemistry) (Immunochemical staining of human CD3E in cynomolgus macaque lymphnode with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).)

IHC (Immunohistochemistry) (Immunochemical staining of human CD3E in cynomolgus macaque tonsil with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).)

IHC (Immunohistochemistry) (Immunochemical staining CD3E in human tonsil with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).)

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CD3D & CD3E Heterodimer, Monoclonal Antibody (Cat# AAA27755)

Full Name

Recombinant Anti-CD3D & CD3E Heterodimer Antibody, Rabbit Monoclonal

Gene Names

CD3D; T3D; IMD19; CD3-DELTA

Reactivity

Human, Cynomolgus

Applications

Immunohistochemistry, Flow Cytometry

Purity

Protein A

Pricing

$225/0.02 mL $405/0.1 mL $760/2x0.1 mL $1,105/3x0.1 mL $1,420/4x0.1 mL $1,520/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of Human Lysates showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:1000. Comparison of clone H9010 behavior with Hsp90 human beta (1) and Hsp90 human alpha (2). Courtesy of: David Toft, Mayo Clinic.)

WB (Western Blot) (Western Blot analysis of Human Cervical cancer cell line (HeLa) lysate showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:1000. Secondary Antibody: HRP Goat Anti-Mouse.)

IHC (Immunohistchemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Tissue: backskin. Species: Mouse. Fixation: Bouin's Fixative and paraffin-embedded. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:100 for 1 hour at RT. Secondary Antibody: FITC Goat Anti-Mouse (green) at 1:50 for 1 hour at RT. Localization: Epidermis.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Alexa Fluor 555 Goat Anti-Mouse (red) at 1:5000 for 1 hour at RT. Magnification: 40x.)

WB (Western Blot) (Western Blot analysis of Human cell lysates from various cell lines showing detection of Hsp90 protein using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Load: 15 ug. Block: 1.5% BSA for 30 minutes at RT. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:1000 for 2 hours at RT. Secondary Antibody: Sheep Anti-Mouse IgG: HRP for 1 hour at RT.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Tissue: colon carcinoma. Species: Human. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Mouse at 1:2000 for 1 hour at RT. Counterstain: Mayer Hematoxylin (purple/blue) nuclear stain at 200 ul for 2 minutes at RT. Localization: Inflammatory cells. Magnification: 40x.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis using Mouse Anti-Hsp90 Monoclonal Antibody, Clone H9010. Tissue: inflamed colon. Species: Mouse. Fixation: Formalin. Primary Antibody: Mouse Anti-Hsp90 Monoclonal Antibody at 1:10000 for 12 hours at 4 degree C. Secondary Antibody: Alexa Fluor 555 Goat Anti-Mouse (red) at 1:5000 for 1 hour at RT. Localization: Inflammatory and epithelial mucosa. Magnification: 40x. Inflammatory and epithelial mucosa.)

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Hsp90, Monoclonal Antibody (Cat# AAA17788)

Full Name

Hsp90 Antibody: PerCP

Gene Names

HSP90AB1; HSP84; HSPC2; HSPCB; D6S182; HSP90B

Reactivity

Human (beta-specific), Rabbit (Beta Specific), Chicken (Alpha/Beta), Rat, Canine

Applications

WB, IP, EIA, IHC, IF

Pricing

$680/0.2 mg $1,300/2x0.2 mg $1,890/3x0.2 mg $2,440/4x0.2 mg $2,680/5x0.2 mg

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WB (Western Blot) (Western blot analysis of CAMK4 over-expressed 293 cell line, cotransfected with CAMK4 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CAMK4 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CAMK4 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CAMK4 on formalin-fixed paraffin-embedded human tonsil tissue. [antibody concentration 5ug/ml].)

WB (Western Blot) (Western Blot analysis of CAMK4 expression in transfected 293T cell line by CAMK4 monoclonal antibody. Lane 1: CAMK4 transfected lysate (52.14kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (CAMK4 monoclonal antibody Western Blot analysis of CAMK4 expression in Jurkat.)

WB (Western Blot) (Western Blot detection against Immunogen (77.66kD).)

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CAMKIV, Monoclonal Antibody (Cat# AAA25040)

Full Name

CAMKIV (Calcium/Calmodulin-dependent Protein Kinase Type IV, CAM Kinase-GR, CaMK IV, CAMK4) (FITC)

Gene Names

CAMK4; caMK; CaMKIV; CaMK IV; CaMK-GR

Reactivity

Human

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (AKR1B10 monoclonal antibody, Western Blot analysis of AKR1B10 expression in HepG2.)

Application Data (Detection limit for recombinant GST tagged AKR1B10 is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to AKR1B10 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to AKR1B10 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of AKR1B10 expression in transfected 293T cell line by AKR1B10 monoclonal antibody. Lane 1: AKR1B10 transfected lysate (36kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (33.59kD).)

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AKR1B10, Monoclonal Antibody (Cat# AAA25315)

Full Name

AKR1B10 (Aldo-keto Reductase Family 1 Member B10, ARL-1, Aldose Reductase-like, Aldose Reductase-related Protein, ARP, hARP, Small Intestine Reductase, SI Reductase, AKR1B11) (HRP)

Gene Names

AKR1B10; HIS; HSI; ARL1; ARL-1; ALDRLn; AKR1B11; AKR1B12

Reactivity

Human

Applications

EIA, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IF (Immunofluorescence) (Figure 8. IF analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (AAA19662).CD147/Emmprin was detected in a paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 ug/mL mouse anti-CD147/Emmprin Antibody (AAA19662) overnight at 4 degree C. Biotin conjugated goat anti-mouse IgG (BA1001) was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using DyLight488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (AAA19662).CD147/Emmprin was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-CD147/Emmprin Antibody (AAA19662) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (AAA19662).CD147/Emmprin was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-CD147/Emmprin Antibody (AAA19662) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (AAA19662).CD147/Emmprin was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-CD147/Emmprin Antibody (AAA19662) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (AAA19662).CD147/Emmprin was detected in a paraffin-embedded section of human laryngeal squamous cell carcinomas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-CD147/Emmprin Antibody (AAA19662) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (AAA19662).CD147/Emmprin was detected in a paraffin-embedded section of human colorectal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-CD147/Emmprin Antibody (AAA19662) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (AAA19662).CD147/Emmprin was detected in a paraffin-embedded section of human breast infiltrating ductal carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-CD147/Emmprin Antibody (AAA19662) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of CD147/Emmprin using anti-CD147/Emmprin antibody (AAA19662).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human Jurkat whole cell lysates,Lane 3: human HepG2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-CD147/Emmprin antigen affinity purified monoclonal antibody (#AAA19662) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CD147/Emmprin at approximately 35-60 kDa. The expected band size for CD147/Emmprin is at 42 kDa.)

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CD147/Emmprin, Monoclonal Antibody (Cat# AAA19662)

Full Name

Anti-CD147/Emmprin Antibody Picoband (monoclonal, 6H2B2)

Gene Names

BSG; M6; OK; 5F7; TCSF; CD147; EMMPRIN

Reactivity

Human, Mouse

Applications

WB, IHC-P, IF, ICC

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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IHC (Immunohistochemistry) (SNX8 Antibody-IHC of paraffin-embedded Human liver tissue using anti-SNX8 mouse monoclonal antibody.)

IHC (Immunohistchemistry) (SNX8 Antibody-IHC of paraffin-embedded Carcinoma of Human kidney tissue using anti-SNX8 mouse monoclonal antibody.)

IHC (Immunohistochemistry) (SNX8 Antibody-IHC of paraffin-embedded Human Kidney tissue using anti-SNX8 mouse monoclonal antibody.)

IHC (Immunohistochemistry) (SNX8 Antibody-IHC of paraffin-embedded Carcinoma of Human lung tissue using anti-SNX8 mouse monoclonal antibody.)

IHC (Immunohistchemistry) (SNX8 Antibody-IHC of paraffin-embedded Human lymphoma tissue using anti-SNX8 mouse monoclonal antibody.)

IHC (Immunohistochemistry) (SNX8 Antibody-IHC of paraffin-embedded Human lymph node tissue using anti-SNX8 mouse monoclonal antibody.)

IHC (Immunohistochemistry) (SNX8 Antibody-IHC of paraffin-embedded Human thyroid tissue using anti-SNX8 mouse monoclonal antibody.)

IHC (Immunohistochemistry) (SNX8 Antibody-Human Tonsil: Formalin-Fixed, Paraffin-Embedded (FFPE), at a concentration of 10 ug/ml.)

Application Data (SNX8 Antibody-Western blot of extracts (35 ug) from 9 different cell lines by using anti-SNX8 monoclonal antibody.)

ICC (Immunocytochemistry) (SNX8 Antibody-Anti-SNX8 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY SNX8.)

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SNX8, Monoclonal Antibody (Cat# AAA21335)

Full Name

SNX8 Mouse anti-Human Monoclonal (4F8) Antibody

Gene Names

SNX8; Mvp1

Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunohistochemistry, Western Blot

Purity

Protein A/G purified

Pricing

$690/0.05 mL $1,325/2x0.05 mL $1,915/3x0.05 mL $2,475/4x0.05 mL $2,800/5x0.05 mL

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WB (Western Blot) (Detection of mouse SMAD4 by western blot. Samples: Whole cell lysate (30 ug) from NIH 3T3, CT26, CH27, C2C12, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SMAD4 recombinant monoclonal antibody (AAA23844 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)

WB (Western Blot) (Detection of human SMAD4 by western blot. Samples: Whole cell lysate (1 ug) from HEK293T, GaMG, A-549, HeLa, and Hep-G2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-SMAD4 recombinant monoclonal antibody (AAA23844 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody .)

IP (Immunoprecipitation) (Detection of human SMAD4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from 293T cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-SMAD4 recombinant monoclonal antibody (AAA23844 lot 1) used for IP at 6 ul per reaction. SMAD4 was also immunoprecipitated by rabbit anti-SMAD4 antibody BL-1681B-1A10. For blotting immunoprecipitated SMAD4, AAA23844 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)

IHC (Immunohistochemistry) (Detection of human SMAD4 in FFPE lung carcinoma by immunohistochemistry. Antibody: Rabbit anti-SMAD4 recombinant monoclonal antibody (AAA23844 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry) (Detection of human SMAD4 in FFPE RPMI-8226 cells by immunocytochemistry. Antibody: Rabbit anti-SMAD4 recombinant monoclonal antibody (AAA23844 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry) (Detection of human SMAD4 in FFPE Jurkat cells by immunocytochemistry. Antibody: Rabbit anti-SMAD4 recombinant monoclonal antibody (AAA23844 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

FCM (Flow Cytometry) (Detection of human Smad4 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-Smad4 recombinant monoclonal (AAA23844 lot 1) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)

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SMAD4, Monoclonal Recombinant Antibody (Cat# AAA23844)

Full Name

Rabbit anti-SMAD4 Recombinant Monoclonal Antibody [BLR133J]

Gene Names

SMAD4; JIP; DPC4; MADH4; MYHRS

Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry

Purity

Purified

Pricing

$295/0.01 mL $680/0.1 mL $980/5x0.01 mL $1,300/2x0.1 mL $1,890/3x0.1 mL $2,440/4x0.1 mL $2,755/5x0.1 mL

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Application Data (Proximity Ligation Analysis of protein-protein interactions between CRKL and PIK3R1. Mahlavu cells were stained with anti-CRKL rabbit purified polyclonal 1:1200 and anti-PIK3R1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)

Application Data (Proximity Ligation Analysis of protein-protein interactions between FLT1 and PIK3R1 Huh7 cells were stained with anti-FLT1 rabbit purified polyclonal 1:1200 and anti-PIK3R1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)

Application Data (Proximity Ligation Analysis of protein-protein interactions between EGFR and PIK3R1. HeLa cells were stained with EGFR rabbit purified polyclonal 1:1200 and PIK3R1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue))

Application Data (Detection limit for recombinant GST tagged PIK3R1 is ~0.1ng/ml as a capture antibody.)

WB (Western Blot) (PIK3R1 monoclonal antibody Western Blot analysis of PIK3R1 expression in PC-12.)

WB (Western Blot) (PIK3R1 monoclonal antibody Western Blot analysis of PIK3R1 expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (75.68kD).)

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PIK3R1, Monoclonal Antibody (Cat# AAA25498)

Full Name

PIK3R1 (GRB1, Phosphatidylinositol 3-kinase Regulatory Subunit alpha, Phosphatidylinositol 3-kinase 85kD Regulatory Subunit alpha) (HRP)

Gene Names

PIK3R1; p85; AGM7; GRB1; IMD36; p85-ALPHA

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (CLK3 monoclonal antibody. Western Blot analysis of CLK3 expression in Hela NE.)

WB (Western Blot) (Western blot analysis of CLK3 over-expressed 293 cell line, cotransfected with CLK3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CLK3 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CLK3 on HeLa cell. [antibody concentration 8ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CLK3 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.5ug/ml].)

WB (Western Blot) (Western Blot analysis of CLK3 expression in transfected 293T cell line by CLK3 monoclonal antibody. Lane 1: CLK3 transfected lysate (58.6kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

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CLK3, Monoclonal Antibody (Cat# AAA25354)

Full Name

CLK3 (Dual Specificity Protein Kinase CLK3, CDC-like Kinase 3) (HRP)

Gene Names

CLK3; PHCLK3; PHCLK3/152

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (G3BP monoclonal antibody Western Blot analysis of G3BP expression in A-431)

WB (Western Blot) (Western blot analysis of G3BP over-expressed 293 cell line, cotransfected with G3BP Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with G3BP monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to G3BP on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to G3BP on formalin-fixed paraffin-embedded human lymphoma. [antibody concentration 1ug/ml])

WB (Western Blot) (Western Blot analysis of G3BP expression in transfected 293T cell line by G3BP monoclonal antibody Lane 1: G3BP transfected lysate (52.2kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)

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G3BP1, Monoclonal Antibody (Cat# AAA25400)

Full Name

G3BP1 (Ras GTPase-activating Protein-binding Protein 1, G3BP-1, ATP-dependent DNA Helicase VIII, hDH VIII, GAP SH3 Domain-binding Protein 1, G3BP) (HRP)

Gene Names

G3BP1; G3BP; HDH-VIII

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western blot analysis of SGK over-expressed 293 cell line, cotransfected with SGK Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with SGK monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged SGK is ~1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SGK on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SGK on formalin-fixed paraffin-embedded human stomach tissue. [antibody concentration 5ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SGK on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of SGK expression in transfected 293T cell line by SGK monoclonal antibody. Lane 1: SGK transfected lysate (48.9kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (73.52kD).)

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SGK, Monoclonal Antibody (Cat# AAA25245)

Full Name

SGK (SGK1, Serine/Threonine-protein Kinase SGK1, Serum/Glucocorticoid-regulated Kinase 1) (FITC)

Gene Names

SGK1; SGK

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged GLUD2 is 0.3 ng/ml as a capture antibody.)

WB (Western Blot) (GLUD2 monoclonal antibody (M01), clone 3C2. Western Blot analysis of GLUD2 expression in human pancreas.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GLUD2 on formalin-fixed paraffin-embedded human ovarian cancer. [antibody concentration 1 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GLUD2 on formalin-fixed paraffin-embedded human ovarian cancer. [antibody concentration 1 ug/ml])

IP (Immunoprecipitation) (Immunoprecipitation of GLUD2 transfected lysate using anti-GLUD2 monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with GLUD2 MaxPab rabbit polyclonal antibody.)

WB (Western Blot) (Western Blot analysis of GLUD2 expression in transfected 293T cell line by GLUD2 monoclonal antibody (M01), clone 3C2.Lane 1: GLUD2 transfected lysate (61 KDa).Lane 2: Non-transfected lysate.)

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GLUD2, Monoclonal Antibody (Cat# AAA26539)

Full Name

GLUD2 (Glutamate Dehydrogenase 2, GDH2, GLUDP1) (HRP)

Gene Names

GLUD2; GDH2; GLUDP1

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (KLF10 monoclonal antibody (M15), clone 1H3. Western Blot analysis of KLF10 expression in PC-12.)

WB (Western Blot) (KLF10 monoclonal antibody (M15), clone 1H3. Western Blot analysis of KLF10 expression in NIH/3T3.)

WB (Western Blot) (KLF10 monoclonal antibody (M15), clone 1H3. Western Blot analysis of KLF10 expression in Jurkat.)

WB (Western Blot) (KLF10 monoclonal antibody (M15), clone 1H3. Western Blot analysis of KLF10 expression in A-549 (Cat # L025V1).)

WB (Western Blot) (KLF10 monoclonal antibody (M15), clone 1H3. Western Blot analysis of KLF10 expression in human colon.)

WB (Western Blot) (KLF10 monoclonal antibody (M15), clone 1H3. Western Blot analysis of KLF10 expression in Y-79 (Cat # L042V1).)

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KLF10, Monoclonal Antibody (Cat# AAA26398)

Full Name

KLF10 (Kruppel-like Factor 10, EGRA, TIEG, TIEG1) (FITC)

Gene Names

KLF10; EGRA; TIEG; TIEG1; EGR-alpha

Applications

Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (NEK2 monoclonal antibody (M11), clone 3B7. Western Blot analysis of NEK2 expression in PC-12 (Cat # L012V1).)

WB (Western Blot) (NEK2 monoclonal antibody (M11), clone 3B7. Western Blot analysis of NEK2 expression in NIH/3T3 (Cat # L018V1).)

Application Data (Detection limit for recombinant GST tagged NEK2 is approximately 3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NEK2 on HeLa cell. [antibody concentration 25 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NEK2 on HeLa cell. [antibody concentration 25 ug/ml])

WB (Western Blot) (NEK2 monoclonal antibody (M11), clone 3B7 Western Blot analysis of NEK2 expression in MCF-7 (Cat # L046V1).)

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NEK2, Monoclonal Antibody (Cat# AAA26431)

Full Name

NEK2 (NIMA (never in Mitosis Gene a)-Related Kinase 2, HsPK21, NEK2A, NLK1) (HRP)

Gene Names

NEK2; NLK1; RP67; NEK2A; HsPK21; PPP1R111

Applications

Immunofluorescence, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western blot analysis of GSC over-expressed 293 cell line, cotransfected with GSC Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with GSC monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged GSC is ~0.3ng/ml as a capture antibody.)

WB (Western Blot) (Western Blot analysis of GSC expression in transfected 293T cell line by GSC monoclonal antibody. Lane 1: GSC transfected lysate (28.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (GSC monoclonal antibody. Western Blot analysis of GSC expression in SJCRH30.)

WB (Western Blot) (GSC monoclonal antibody. Western Blot analysis of GSC expression in COLO 320 HSR.)

WB (Western Blot) (Western Blot detection against Immunogen (37.88kD).)

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GSC, Monoclonal Antibody (Cat# AAA24229)

Full Name

GSC (Homeobox Protein Goosecoid) (AP)

Gene Names

GSC; SAMS

Reactivity

Human

Applications

EIA, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b)

Application Data (Staining of mouse peritoneal macrophages cells with Rat anti Mouse CD11b:Alexa Fluor 488)

Application Data (Staining of total mouse peritoneal exudate cells demonstrating labelling of macrophages with Rat anti Mouse CD11b:RPE)

Application Data (Published customer image: Immunocytochemical characterisation of cultures of mouse microglia. Cultures of microglia were immunostained with anti-CD11b (a) and anti-GFAP (c). Cultures of astrocytes were immunostained with anti-CD11b (c) and anti-GFAP (d). All cells were also counterstained with DAPI (blue) to identify the cells' nuclei.From: Ferger et al. Journal of Neuroinflammation 2010 7:45)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b:RPE)

Application Data (Staining of mouse peritoneal macrophages cells with Rat anti Mouse CD11b:FITC)

Application Data (Immunoperoxidase staining of mouse lymph node cryosection using Rat anti Mouse CD11b antibody followed by horseradish peroxidase conjugated Goat anti Rat IgG . High power)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b: Alexa Fluor 647)

Application Data (Immunoperoxidase staining of mouse lymph node cryosection using Rat anti Mouse CD11b antibody followed by horseradish peroxidase conjugated Goat anti Rat IgG . Medium power)

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CD11b, Monoclonal Antibody (Cat# AAA12008)

Full Name

RAT ANTI MOUSE CD11b

Gene Names

Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik

Applications

Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry

Pricing

$440/0.1 mg $840/2x0.1 mg $1,205/3x0.1 mg $1,555/4x0.1 mg $1,680/5x0.1 mg

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WB (Western Blot) (NADK monoclonal antibody. Western Blot analysis of NADK expression in HepG2.)

WB (Western Blot) (NADK monoclonal antibody, Western Blot analysis of NADK expression in HeLa.)

Application Data (Detection limit for recombinant GST tagged NADK is ~0.3ng/ml as a capture antibody.)

WB (Western Blot) (Western Blot analysis of NADK expression in transfected 293T cell line by NADK monoclonal antibody. Lane 1: NADK transfected lysate (49.2kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (NADK monoclonal antibody. Western Blot analysis of NADK expression in NIH/3T3.)

WB (Western Blot) (NADK monoclonal antibody. Western Blot analysis of NADK expression in Raw 264.7.)

WB (Western Blot) (Western Blot detection against Immunogen (75.17kD).)

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NADK, Monoclonal Antibody (Cat# AAA24578)

Full Name

NADK (NAD Kinase, Poly(P)/ATP NAD Kinase, dJ283E3.1, DKFZp686L22239, FLJ13052, FLJ37724, FLJ54695, FLJ77769, FLJ78247, FLJ78307, MGC1900) APC

Gene Names

NADK; dJ283E3.1

Reactivity

Human, Mouse

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (RNF12 monoclonal antibody, Western Blot analysis of RNF12 expression in HeLa NE.)

Application Data (Detection limit for recombinant GST tagged RNF12 is ~0.03ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of RNF12 transfected lysate using RNF12 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with RNF12 rabbit polyclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RNF12 on HeLa cell. [antibody concentration 30ug/ml].)

WB (Western Blot) (Western Blot analysis of RNF12 expression in transfected 293T cell line by RNF12 monoclonal antibody. Lane 1: RNF12 transfected lysate (68.5kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (35.24kD).)

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RLIM, Monoclonal Antibody (Cat# AAA25227)

Full Name

RLIM (RING Finger LIM Domain-binding Protein, R-LIM, E3 Ubiquitin-protein Ligase RLIM, LIM Domain-interacting RING Finger Protein, RING Finger Protein 12, RNF12, Renal Carcinoma Antigen NY-REN-43) (FITC)

Gene Names

RLIM; MRX61; RNF12; TOKAS; NY-REN-43

Reactivity

Human

Applications

Immunofluorescence, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to POU5F1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to POU5F1 on HeLa cell. [antibody concentration 10 ug/ml])

Application Data (A-549 cells were stained with POU5F1-FITC labeled monoclonal antibody (Green). The cell nucleus were counterstained with DAPI (Blue).)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to POU5F1 on A-549 cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (Western Blot analysis of POU5F1 expression in transfected 293T cell line by POU5F1 monoclonal antibody (M05), clone 1B11.Lane 1: POU5F1 transfected lysate (18.3 KDa).Lane 2: Non-transfected lysate.)

WB (Western Blot) (POU5F1 monoclonal antibody (M05), clone 1B11 Western Blot analysis of POU5F1 expression in HepG2 (Cat # L019V1).)

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POU5F1, Monoclonal Antibody (Cat# AAA26627)

Full Name

POU5F1 (POU Class 5 Homeobox 1, MGC22487, OCT3, OCT4, OTF3, OTF4) (PE)

Applications

Immunofluorescence, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (USF2 monoclonal antibody (M03), clone 6A9. Western Blot analysis of USF2 expression in PC-12 (Cat # L012V1).)

Application Data (Detection limit for recombinant GST tagged USF2 is approximately 0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to USF2 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to USF2 on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (USF2 monoclonal antibody (M03), clone 6A9. Western Blot analysis of USF2 expression in different cell lines.)

WB (Western Blot) (USF2 monoclonal antibody (M03), clone 6A9. Western Blot analysis of USF2 expression in Hela S3 NE (Cat # L013V3).)

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USF2, Monoclonal Antibody (Cat# AAA26640)

Full Name

USF2 (Upstream Transcription Factor 2, c-fos Interacting, FIP, bHLHb12) (PE)

Gene Names

USF2; FIP; bHLHb12

Applications

Immunofluorescence, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of LOVO cells with Wnt2b antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining Wnt2b in SHSY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Wnt2b in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Wnt2b in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Wnt2b antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Wnt2b antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Wnt2b antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Wnt2b on SiHa cell lysates using anti-Wnt2b at 1/500 dilution.)

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Wnt2b, Monoclonal Antibody (Cat# AAA30442)

Full Name

Wnt2b Antibody

Gene Names

WNT2B; WNT13

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of SH-SY-5Y cells with PSD95 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining PSD95 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining PSD95 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse cerebullum tissue using anti-PSD95 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-PSD95 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PSD95 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of PSD95 on different lysates using anti-PSD95 antibody at 1/1, 000 dilution. Positive control: Lane 1: Mouse brain Lane 2: Human brain)

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PSD95, Monoclonal Antibody (Cat# AAA29987)

Full Name

PSD95 Antibody

Gene Names

DLG4; PSD95; SAP90; SAP-90

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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WB (Western Blot) (AKT2 monoclonal antibody (M05), clone 1F3. Western Blot analysis of AKT2 expression in PC-12.)

WB (Western Blot) (AKT2 monoclonal antibody (M05), clone 1F3. Western Blot analysis of AKT2 expression in NIH/3T3.)

Application Data (Detection limit for recombinant GST tagged AKT2 is approximately 1ng/ml as a capture antibody.)

WB (Western Blot) (AKT2 monoclonal antibody (M05), clone 1F3 Western Blot analysis of AKT2 expression in Jurkat (Cat # L017V1).)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to AKT2 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to AKT2 on HeLa cell. [antibody concentration 10 ug/ml])

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AKT2, Monoclonal Antibody (Cat# AAA26367)

Full Name

AKT2 (v-akt Murine Thymoma Viral Oncogene Homolog 2, PKBB, PKBBETA, PRKBB, RAC-BETA) (FITC)

Gene Names

AKT2; PKBB; PRKBB; HIHGHH; PKBBETA; RAC-BETA

Applications

IF, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

What are Monoclonal Antibodies?

Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.

This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.

AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.

What Are The Uses of Monoclonal Antibodies

Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.

ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.

Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.

Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.

Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.

How Monoclonal Antibodies Are Used as Medicine?

Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).

Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:

• Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.


• Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.


• Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.

Why Buy Monoclonal Antibodies From Us?

At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.

1. Validated for Versatile Applications

The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).

2. Wide Selection & Specialized Options

We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.

3. High-Quality Proteins

Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.

Frequently Asked Questions

1. Are your monoclonal antibodies validated for specific applications?

Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.

2. How do I choose the right monoclonal antibody for my application?

Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.

3. How quickly can I receive my order?

Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.