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WB (Western Blot) (DARS monoclonal antibody Western Blot analysis of DARS expression in HeLa.)
WB (Western Blot) (Western blot analysis of DARS over-expressed 293 cell line, cotransfected with DARS Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with DARS monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged DARS is ~0.3ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to DARS on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of DARS expression in transfected 293T cell line by DARS monoclonal antibody. Lane 1: DARS transfected lysate (57.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (37.62kD).)
WB (Western Blot) (SPRY2 monoclonal antibody Western Blot analysis of SPRY2 expression in C32)
Application Data (Detection limit for recombinant GST tagged SPRY2 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SPRY2 on HeLa cell. [antibody concentration 25ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SPRY2 on formalin-fixed paraffin-embedded human lymphoma. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of SPRY2 expression in transfected 293T cell line by SPRY2 monoclonal antibody Lane 1: SPRY2 transfected lysate (34.7kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (60.76kD).)
WB (Western Blot) (ACATE2 monoclonal antibody Western Blot analysis of ACATE2 expression in MCF-7.)
Application Data (Detection limit for recombinant GST tagged ACOT9 is ~3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ACOT9 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ACOT9 on formalin-fixed paraffin-embedded human heart. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of ACOT9 expression in transfected 293T cell line by ACATE2 monoclonal antibody. Lane 1: ACOT9 transfected lysate (24kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (49.43kD).)
WB (Western Blot) (P15RS monoclonal antibody. Western Blot analysis of P15RS expression in NIH/3T3.)
Application Data (Detection limit for recombinant GST tagged P15RS is ~0.03ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to P15RS on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3ug/ml].)
WB (Western Blot) (P15RS monoclonal antibody, Western Blot analysis of P15RS expression in A-431.)
WB (Western Blot) (P15RS monoclonal antibody. Western Blot analysis of P15RS expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (36.56kD).)
WB (Western Blot) (HSPA1L monoclonal antibody Western Blot analysis of HSPA1L expression in PC-12.)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between MAP3K7 and HSPA1L HeLa cells were stained with MAP3K7 rabbit purified polyclonal 1:1200 and HSPA1L mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Application Data (Detection limit for recombinant GST tagged HSPA1L is ~0.1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HSPA1L on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml])
WB (Western Blot) (HSPA1L monoclonal antibody Western Blot analysis of HSPA1L expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (34.65kD).)
WB (Western Blot) (RIPK2 monoclonal antibody (M02), clone 6F7. Western Blot analysis of RIPK2 expression in PC-12 (Cat # L012V1).)
WB (Western Blot) (RIPK2 monoclonal antibody (M02), clone 6F7. Western Blot analysis of RIPK2 expression in NIH/3T3 (Cat # L018V1).)
Application Data (Detection limit for recombinant GST tagged RIPK2 is approximately 0.1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RIPK2 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 1.2 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RIPK2 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 1.2 ug/ml])
WB (Western Blot) (RIPK2 monoclonal antibody (M02), clone 6F7 Western Blot analysis of RIPK2 expression in HeLa (Cat # L013V1).)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with AOF1 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
ICC (Immunocytochemistry) (ICC staining AOF1 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human rectum cancer tissue using anti-AOF1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human esophageal cancer tissue using anti-AOF1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of AOF1 on HEK293 (1) and AOF1-hIgGFc transfected HEK293 (2) cell lysate using anti-AOF1 antibody at 1/1, 000 dilution.)
WB (Western Blot) (Western blot analysis of AOF1 on human AOF1 recombinant protein using anti-AOF1 antibody at 1/1, 000 dilution.)
ICC (Immunocytochemistry) (ICC staining ABCF1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining ABCF1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining ABCF1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ABCF1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-ABCF1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-ABCF1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat stomach tissue using anti-ABCF1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of ABCF1 on Hela lysates using anti-ABCF1 antibody at 1/1, 000 dilution.)
WB (Western Blot) (Western blot analysis of ARNT over-expressed 293 cell line, cotransfected with ARNT Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ARNT monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
WB (Western Blot) (Western Blot analysis of ARNT expression in transfected 293T cell line by ARNT monoclonal antibody. Lane 1: ARNT transfected lysate (86.6kD). Lane 2: Non-transfected lysate.)
Application Data (Detection limit for recombinant GST tagged ARNT is ~0.03ng/ml as a capture antibod)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ARNT on HeLa cell. [antibody concentration 10ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ARNT on formalin-fixed paraffin-embedded human lung. [antibody concentration 3ug/ml].)
WB (Western Blot) (ARNT monoclonal antibody Western Blot analysis of ARNT expression in Hela NE.)
WB (Western Blot) (Western Blot detection against Immunogen (37.73kD).)
Application Data (Detection limit for recombinant GST tagged GTF2H1 is ~1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GTF2H1 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GTF2H1 on formalin-fixed paraffin-embedded human testis tissue.[antibody concentration 5ug/ml])
WB (Western Blot) (Western Blot analysis of GTF2H1 expression in transfected 293T cell line by GTF2H1 monoclonal antibody. Lane 1: GTF2H1 transfected lysate (62kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (GTF2H1 monoclonal antibody Western Blot analysis of GTF2H1 expression in Jurkat.)
WB (Western Blot) (GTF2H1 monoclonal antibody Western Blot analysis of GTF2H1 expression in Hela NE.)
WB (Western Blot) (Western Blot detection against Immunogen (86.02kD).)
WB (Western Blot) (Western Blot analysis of GABPA expression in transfected 293T cell line by GABPA monoclonal antibody (M03), clone M1.Lane 1: GABPA transfected lysate (Predicted MW: 51.3 KDa).Lane 2: Non-transfected lysate.)
IP (Immunoprecipitation) (Immunoprecipitation of GABPA transfected lysate using anti-GABPA monoclonal antibody and Protein A Magnetic Bead (U0007), and immunoblotted with GABPA monoclonal antibody.)
Application Data (Detection limit for recombinant GST tagged GABPA is approximately 1ng/ml as a capture antibody.)
WB (Western Blot) (GABPA monoclonal antibody (M03), clone M1 Western Blot analysis of GABPA expression in Hela S3 NE (Cat # L013V3).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GABPA on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GABPA on HeLa cell. [antibody concentration 10 ug/ml])
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD204: Alexa Fluor 488)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD204: Low Endotoxin)
Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD204 antibody, clone 2F8 followed by horseradish peroxidase Goat anti Rat IgG antibody . Low power)
Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD204 antibody, clone 2F8 followed by horseradish peroxidase Goat anti Rat IgG antibody . Medium power)
Application Data (Staining of mouse peritoneal macrophages cells with Rat anti Mouse CD204:FITC)
Application Data (Western Blot staining of J774 lysate (non reduced) probed with Rat anti Mouse CD204)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD204: Biotin)
Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD204 antibody, clone 2F8 followed by horseradish peroxidase Goat anti Rat IgG antibody . High power)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD204: Alexa Fluor 647)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD204:FITC)
FCM (Flow Cytometry) (Flow cytometric analysis of K562 cells with Proteasome 20S C2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Proteasome 20S C2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-Proteasome 20S C2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-Proteasome 20S C2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Proteasome 20S C2 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Proteasome 20S C2 on different lysates using anti-Proteasome 20S C2 antibody at 1/500 dilution. Positive control: Lane 1: PC-3M Lane 2: K562 Lane 3: Rat testis Lane 4: Mouse testis Lane 5: Mouse colon)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD43: FITC)
Application Data (Imunofluorescence staining of human tonsil cryosection with Mouse anti Human CD43, clone DFT-1, red in A and Mouse anti Human CD21 , green in B. C is the merged image with nuclei counterstained blue using DAPI. High power)
Application Data (Immunoperoxidase staining of human tonsil cryosection with Mouse anti Human CD43 antibody, clone DFT-1 followed by the HISTAR detection system . Medium power)
Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD43)
Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD43:Biotin)
Application Data (Immunoperoxidase staining of human tonsil cryosection with Mouse anti Human CD43 antibody, clone DFT-1 followed by the HISTAR detection system . Low power)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD43:Azide Free)
Application Data (Imunofluorescence staining of human tonsil cryosection with Mouse anti Human CD43, clone DFT-1, red in A and Mouse anti Human CD21 , green in B. C is the merged image with nuclei counterstained blue using DAPI. Medium power)
Application Data (Immunoperoxidase staining of human tonsil cryosection with Mouse anti Human CD43 antibody, clone DFT-1 followed by the HISTAR detection system . Medium power)
Application Data (Imunofluorescence staining of human tonsil cryosection with Mouse anti Human CD43, clone DFT-1, red in A and Mouse anti Human CD21 , green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power)
Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . Medium power)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD314: Alexa Fluor 488)
Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . High power)
Application Data (Immunoperoxidase staining of a human spleen cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . high power)
Application Data (Immunoperoxidase staining of a human spleen cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . Low power)
Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . Low power)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD314)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD314:Low Endotoxin)
WB (Western Blot) (FLJ23356 monoclonal antibody. Western Blot analysis of FLJ23356 expression in HeLa.)
WB (Western Blot) (FLJ23356 monoclonal antibody. Western Blot analysis of FLJ23356 expression in HepG2.)
Application Data (Detection limit for recombinant GST tagged FLJ23356 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FLJ23356 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of FLJ23356 expression in transfected 293T cell line by FLJ23356 monoclonal antibody. Lane 1: FLJ23356 transfected lysate (38.5kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western blot analysis of FLJ23356 over-expressed 293 cell line, cotransfected with FLJ23356 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with FLJ23356 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
Application Data (Detection limit for recombinant GST tagged PEPD is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of PEPD transfected lysate using PEPD monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PEPD rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PEPD on HepG2 cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of PEPD expression in transfected 293T cell line by PEPD monoclonal antibody. Lane 1: PEPD transfected lysate (Predicted MW: 54.6kD). Lane 2: Non-transfected lysate)
WB (Western Blot) (PEPD monoclonal antibody Western Blot analysis of PEPD expression in HepG2.)
WB (Western Blot) (Western Blot detection against Immunogen (79.97kD).)
WB (Western Blot) (Western Blot detection against Immunogen (51.96kD).)
Application Data (Detection limit for recombinant GST tagged FAM3B is 1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of FAM3B transfected lysate using FAM3B monoclonal antibody and Protein A Magnetic Bead and immunoblotted with FAM3B rabbit polyclonal antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FAM3B on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of FAM3B expression in transfected 293T cell line by FAM3B monoclonal antibody. Lane 1: FAM3B transfected lysate (26kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (FAM3B monoclonal antibody. Western Blot analysis of FAM3B expression in human kidney.)
FCM (Flow Cytometry) (Flow cytometric analysis of SH-SY-5Y cells with STUB1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining STUB1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining STUB1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining STUB1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-STUB1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-STUB1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-STUB1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-STUB1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of STUB1 on different lysates using anti-STUB1 antibody at 1/2, 000 dilution. Positive control: Lane 1: 293 Lane 2: MCF-7 Lane 3: SK-Br-3 Lane 4: Mouse kidney)
FCM (Flow Cytometry) (Flow cytometric analysis of HepG2 cells with Id1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
ICC (Immunocytochemistry) (ICC staining Id1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Id1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Id1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-Id1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Id1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Id1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Id1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Id1 on 293T cell using anti-Id1 antibody at 1/1, 000 dilution.)
FCM (Flow Cytometry) (Flow cytometric analysis of Jurkat cells with ZAP70 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor® 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining ZAP70 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining ZAP70 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-ZAP70 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ZAP70 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of ZAP70 on human thymus tissue lysate using anti-ZAP70 antibody at 1/500 dilution.)
FCM (Flow Cytometry) (Flow cytometric analysis of SH-SY-5Y cells with CRMP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining CRMP1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining CRMP1 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining CRMP1 in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-CRMP1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CRMP1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-CRMP1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of CRMP1 on mouse brain tissue lysate using anti-CRMP1 antibody at 1/500 dilution.)
Application Data (Detection limit for recombinant GST tagged TOMM22 is 0.1ng/ml as a capture.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal to TOMM22 on HeLa)
WB (Western Blot) (Western Blot analysis of TOMM22 expression in Raw)
WB (Western Blot) (Western Blot analysis of TOMM22 expression in PC-12.)
WB (Western Blot) (Western Blot analysis of TOMM22 expression in NIH/3T3.)
IHC (Immunohistochemistry) (TOMM22 (10ug/ml) was used in IHC to stain formalin-fixed, paraffin- embedded human liver, hepatocytes, followed by biotinylated horse, mouse IgG, alkaline phosphatase-streptavidin and chromogen.)
FCM (Flow Cytometry) (Flow cytometry analysis of CD80 overexpressing HEK293 cells using CD80 antibody and control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: CD80 overexpressing HEK293 cells.)
IHC (Immunohistochemistry) (Immunohistochemistry of CD80 in human tonsil tissue with CD80 antibody at 5 μg/mL.)
IHC (Immunohistchemistry) (Immunohistochemistry of CD80 in human stomach carcinoma tissue with CD80 antibody at 5 μg/mL.)
IF (Immunofluorescence) (Immunofluorescence of CD80 in human tonsil tissue with CD80 antibody at 2 μg/mL.Red: CD80 Antibody [12D9]Blue: DAPI staining)
IF (Immunofluorescence) (Immunofluorescence of CD80 in human stomach carcinoma tissue with CD80 antibody at 20 μg/mL.Red: CD80 Antibody [12D9]Blue: DAPI staining)
IF (Immunofluorescence) (Immunofluorescence of CD80 in transfected HEK293 cells with CD80 antibody at 2 μg/mL.Red: CD80 Antibody [12D9]Blue: DAPI staining)
ICC (Immunocytochemistry) (Immunocytochemistry of CD80 in transfected HEK293 cells with CD80 antibody at 1 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 μg/mL.)
WB (Western Blot) (Western blot analysis of CD80 in overexpressing HEK293 cells CD80 antibody at 0.25 and 0.5 μg/ml)
WB (Western Blot) (Detection of mouse BRD9 by western blot. Samples: Whole cell lysate (30 ug) from CT26, CH27, and TCMK-1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD9 recombinant monoclonal antibody (AAA23848 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
WB (Western Blot) (Detection of human BRD9 by western blot. Samples: Whole cell lysate (30 ug) from Jurkat, HEK293T, K-562, MCF-7, and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD9 recombinant monoclonal antibody (AAA23848 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody .)
WB (Western Blot) (Detection of human BRD9 by western blot. Samples: Whole cell lysate (50 ug) prepared using NETN buffer from Jurkat cells (WT and KO). Antibody: Rabbit anti-BRD9 recombinant monoclonal antibody (AAA23848 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)
SWB (Simple Western) (Detection of human BRD9 by Simple Western. Samples: Whole cell lysate (0.4 mg/mL) from Jurkat cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD9 recombinant monoclonal antibody (AAA23848) used at 1:10, 1:50, 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram.)
Simple Western (Detection of mouse BRD9 by Simple Western. Samples: Whole cell lysate (2.0 mg/mL) from CT26 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-BRD9 recombinant monoclonal antibody (AAA23848) used at 1:50 and 1:250. Separation and Detection: SallySue ProteinSimple instrument with the 12-230 kDa separation module and anti-Rabbit detection module. Left Panel: Virtual Lane View. Right Panel: Electropherogram.)
IP (Immunoprecipitation) (Detection of human BRD9 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-BRD9 recombinant monoclonal antibody (AAA23848 lot 2) used for IP at 20 ul/mg lysate. BRD9 was also immunoprecipitated by a previous lot of this antibody (AAA23848 lot 1) and a second antibody against a different epitope of BRD9 . For blotting immunoprecipitated BRD9, AAA23848 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 30 seconds.)
IHC (Immunohistochemistry) (Detection of mouse BRD9 by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Rabbit anti-BRD9 recombinant monoclonal antibody (AAA23848 lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG .)
IHC (Immunohistochemistry) (Detection of human BRD9 by immunohistochemistry. Sample: FFPE section of lung carcinoma. Antibody: Rabbit anti-BRD9 recombinant monoclonal antibody (AAA23848 lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG .)
ICC (Immunocytochemistry) (Detection of human BRD9 by immunocytochemistry. Sample: FFPE section of HDLM-2 cells. Antibody: Rabbit anti-BRD9 recombinant monoclonal antibody ((AAA23848 lot 2). Secondary: HRP-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry) (Detection of human BRD9 (shaded) in HDLM-2 cells by flow cytometry. Antibody: Rabbit anti-BRD9 recombinant monoclonal antibody (AAA23848) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry) (Detection of mouse BRD9 (shaded) in NIH3T3 cells by flow cytometry. Antibody: Rabbit anti-BRD9 recombinant monoclonal antibody (AAA23848) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
WB (Western Blot) (RRAS2 monoclonal antibody Western Blot analysis of RRAS2 expression in A-431.)
WB (Western Blot) (RRAS2 monoclonal antibody Western Blot analysis of RRAS2 expression in NIH/3T3)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RRAS2 on A-431 cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RRAS2 on formalin-fixed paraffin-embedded human dysgerminoma tissue. [antibody concentration 5ug/ml])
WB (Western Blot) (Western Blot analysis of RRAS2 expression in transfected 293T cell line by RRAS2 monoclonal antibody Lane 1: RRAS2 transfected lysate (Predicted MW: 23.4kD. Lane 2: Non-transfected lysate.)
WB (Western Blot) (RRAS2 monoclonal antibody Western Blot analysis of RRAS2 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (48.55kD).)
WB (Western Blot) (CDADC1 monoclonal antibody. Western Blot analysis of CDADC1 expression in NIH/3T3.)
WB (Western Blot) (CDADC1 monoclonal antibody. Western Blot analysis of CDADC1 expression in Raw 264.7.)
WB (Western Blot) (Western blot analysis of CDADC1 over-expressed 293 cell line, cotransfected with CDADC1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CDADC1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged CDADC1 is ~0.03ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CDADC1 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1ug/ml].)
WB (Western Blot) (Western Blot analysis of CDADC1 expression in transfected 293T cell line by CDADC1 monoclonal antibody. Lane 1: CDADC1 transfected lysate (58.5kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (36.23kD).)
CDADC1 (Cytidine and dCMP Deaminase Domain-containing Protein 1, Testis Development Protein NYD-SP15, MGC150615, MGC41774, MGC57136, NYD-SP15, BA103J18.1) (PE)
Application Data (Detection limit for recombinant GST tagged PGGT1B is ~1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PGGT1B on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PGGT1B on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1.2ug/ml])
WB (Western Blot) (PGGT1B monoclonal antibody Western Blot analysis of PGGT1B expression in PC-12.)
WB (Western Blot) (PGGT1B monoclonal antibody Western Blot analysis of PGGT1B expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (37.29kD).)
PGGT1B (Geranylgeranyl Transferase Type-1 Subunit beta, Geranylgeranyl Transferase Type I Subunit beta, GGTase-I-beta, Type I Protein Geranyl-Geranyltransferase Subunit beta) (FITC)
Reactivity
Human, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Application Data (Detection limit for recombinant GST tagged GLUD2 is 0.3 ng/ml as a capture antibody.)
WB (Western Blot) (GLUD2 monoclonal antibody (M01), clone 3C2. Western Blot analysis of GLUD2 expression in human pancreas.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GLUD2 on formalin-fixed paraffin-embedded human ovarian cancer. [antibody concentration 1 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GLUD2 on formalin-fixed paraffin-embedded human ovarian cancer. [antibody concentration 1 ug/ml])
IP (Immunoprecipitation) (Immunoprecipitation of GLUD2 transfected lysate using anti-GLUD2 monoclonal antibody and Protein A Magnetic Bead , and immunoblotted with GLUD2 MaxPab rabbit polyclonal antibody.)
WB (Western Blot) (Western Blot analysis of GLUD2 expression in transfected 293T cell line by GLUD2 monoclonal antibody (M01), clone 3C2.Lane 1: GLUD2 transfected lysate(61 KDa).Lane 2: Non-transfected lysate.)
Application Data (Detection limit for recombinant GST tagged UPB1 is 1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to UPB1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of UPB1 expression in transfected 293T cell line by UPB1 monoclonal antibody. Lane 1: UPB1 transfected lysate (43.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (UPB1 monoclonal antibody. Western Blot analysis of UPB1 expression in Jurkat.)
WB (Western Blot) (UPB1 monoclonal antibody. Western Blot analysis of UPB1 expression in human liver.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
Application Data (Detection limit for recombinant GST tagged HMGB2 is approximately 0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HMGB2 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HMGB2 on HeLa cell. [antibody concentration 10 ug/ml])
WB (Western Blot) (HMGB2 monoclonal antibody (M05), clone 3E5 Western Blot analysis of HMGB2 expression in Hela S3 NE (Cat # L013V3).)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HMGB2 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to HMGB2 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml])
CD28 (CD28 Antigen, CD28 Molecule, MGC138290, T cell Antigen CD28, T cell Specific Surface Glycoprotein, T cell Specific Surface Glycoprotein CD28, Tp44) (AP)
ICC (Immunocytochemistry) (ICC staining WSCD2 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-WSCD2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-WSCD2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-WSCD2 antibody. Counter stained with)
WB (Western Blot) (Western blot analysis of WSCD2 on human stomach tissue lysate using anti-WSCD2 antibody at 1/1000 dilution.)
WB (Western Blot) (Western blot analysis of WSCD2 on WSCD2 recombinant protein lysate using anti-WSCD2 antibody at 1/2000 dilution.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using [KD Validated] ATG7 Rabbit mAb (AAA28516) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using [KD Validated] ATG7 Rabbit mAb (AAA28516) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using [KD Validated] ATG7 Rabbit mAb (AAA28516) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human lung cancer tissue using [KD Validated] ATG7 Rabbit mAb (AAA28516) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using [KD Validated] ATG7 Rabbit mAb (AAA28516) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
WB (Western Blot) (Western blot analysis of lysates from wild type(WT) and ATG7 knockdown (KD) HeLa cells using [KD Validated] ATG7 Rabbit mAb (AAA28516) at 1:1000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.)
WB (Western Blot) (Western blot analysis of various lysates using [KD Validated] ATG7 Rabbit mAb (AAA28516) at 1:1000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
WB (Western Blot) (KHDRBS1 monoclonal antibody Western Blot analysis of KHDRBS1 expression in A-431)
Application Data (Detection limit for recombinant GST tagged KHDRBS1 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to KHDRBS1 on A-431 cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to KHDRBS1 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of KHDRBS1 expression in transfected 293T cell line by KHDRBS1 monoclonal antibody. Lane 1: KHDRBS1 transfected lysate (41kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (37kD).)
Application Data (Published clone specific image Alloimmunity-associated cytotoxicity is mediated through the NKG2D receptor. (A) Liver expression of nkg2d on day ten after liver transplantation. (B) Representative NKG2D expression levels in blood NK cells (left) and monocytes (right) of allogeneic (black) and syngeneic (grey) recipients. Isotype was used as control (dashed lines). (C) Sorted blood NK cell cytotoxicity inhibition with anti-NKG2D antibody or with anti-NKp30 antibody. (D) Levels of NKG2D ligand (rae1l, rrlt and irp94) expression in the liver on day ten after transplantation. (E) Levels of NKG2D ligand (rae1l, rrlt and irp94) expression in rat HCC cell lines. (F) Representative level of recombinant NKG2D-Fc binding to rat HCC cells lines. *p)
Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a as a detection reagent. Low power)
Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a as a detection reagent. High power)
Application Data (Immunofluorescence staining of rat lymphnode cryosection with Mouse anti Rat CD172a antibody , red in A and Mouse anti Rat CD4 , green in B. C is the Merged image with nuclei counter-stained blue using DAPI. Medium power)
Application Data (Immunofluorescence staining of rat lymphnode cryosection with Mouse anti Rat CD172a antibody , red in A and Mouse anti Rat CD4 , green in B. C is the Merged image with nuclei counter-stained blue using DAPI. Low power)
Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a as a detection reagent. Medium power)
Application Data (Immunofluorescence staining of rat lymphnode cryosection with Mouse anti Rat CD172a antibody , red in A and Mouse anti Rat CD4 , green in B. C is the Merged image with nuclei counter-stained blue using DAPI. High power)
WB (Western Blot) (Western Blot detection against Immunogen (37kD).)
WB (Western Blot) (RBM9 monoclonal antibody Western Blot analysis of RBM9 expression in NIH/3T3.)
WB (Western Blot) (Western blot analysis of RBM9 over-expressed 293 cell line, cotransfected with RBM9 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with RBM9 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged RBM9 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RBM9 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RBM9 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of RBM9 expression in transfected 293T cell line by RBM9 monoclonal antibody Lane 1: RBM9 transfected lysate (40.4kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western blot analysis of PAX5 over-expressed 293 cell line, cotransfected with PAX5 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PAX5 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for 130905 is ~0.03ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase on formalin-fixed paraffin-embedded human tonsil using 130905 (1.5ug/ml).)
WB (Western Blot) (Western Blot analysis of PAX5 expression in transfected 293T cell line by 130905. Lane 1: PAX5 transfected lysate (42.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot analysis of PAX5 expression in IMR-32 using 130905.)
WB (Western Blot) (Western Blot detection against immunogen (37.84kD).)
Application Data (Proximity Ligation Analysis of protein-protein interactions between CDC6 and MCM7. Huh7 cells were stained with anti-CDC6 rabbit purified polyclonal 1:600 and anti-MCM7 mouse monoclonal antibody 1:100. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
Application Data (Proximity Ligation Analysis of protein-protein interactions between CDK7 and MCM7 HeLa cells were stained with anti-CDK7 rabbit purified polyclonal 1:1200 and anti-MCM7 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
Application Data (Detection limit for recombinant GST tagged MCM7 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MCM7 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to MCM7 on formalin-fixed paraffin-embedded human ovary, clear cell carcinoma. [antibody concentration 3ug/ml])
WB (Western Blot) (MCM7 monoclonal antibody Western Blot analysis of MCM7 expression in Hela NE.)
WB (Western Blot) (Western Blot detection against Immunogen (68.53kD).)
WB (Western Blot) (Western Blot detection against Immunogen (37kD).)
WB (Western Blot) (RBM9 monoclonal antibody Western Blot analysis of RBM9 expression in NIH/3T3.)
WB (Western Blot) (Western blot analysis of RBM9 over-expressed 293 cell line, cotransfected with RBM9 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with RBM9 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged RBM9 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RBM9 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RBM9 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of RBM9 expression in transfected 293T cell line by RBM9 monoclonal antibody Lane 1: RBM9 transfected lysate (40.4kD). Lane 2: Non-transfected lysate.)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with GAB1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining GAB1 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining GAB1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining GAB1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-GAB1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human skin tissue using anti-GAB1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of GAB1 on human small intestine tissue lysate using anti-GAB1 antibody at 1/500 dilution.)
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry
Purity
ProA affinity purified
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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