Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
WB (Western Blot) (FOXA1 monoclonal antibody (M05), clone 3C1. Western Blot analysis of FOXA1 expression in HepG2 (Cat # L019V1).)
WB (Western Blot) (FOXA1 monoclonal antibody (M05), clone 3C1 Western Blot analysis of FOXA1 expression in MCF-7 (Cat # L046V1).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA1 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA1 on HeLa cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FOXA1 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 1.5 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to FOXA1 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 1.5 ug/ml])
ICC (Immunocytochemistry) (ICC staining Histone H4 in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H4 antibody. Counter stained with hematoxylin.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Histone H4 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-Histone H4 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Histone H4 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Histone H4 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H4 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Histone H4 on mouse spleen lysates using anti-Histone H4 antibody at 1/5, 000 dilution.)
WB (Western Blot) (MGC21874 monoclonal antibody. Western Blot analysis of MGC21874 expression in PC-12.)
WB (Western Blot) (MGC21874 monoclonal antibody, Western Blot analysis of MGC21874 expression in HeLa.)
Application Data (Detection limit for recombinant GST tagged MGC21874 is ~0.3ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to MGC21874 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1.5ug/ml].)
WB (Western Blot) (MGC21874 monoclonal antibody. Western Blot analysis of MGC21874 expression in NIH/3T3.)
WB (Western Blot) (MGC21874 monoclonal antibody. Western Blot analysis of MGC21874 expression in Raw 264.7.)
WB (Western Blot) (Western Blot detection against Immunogen (38.1kD).)
WB (Western Blot) (DCPS monoclonal antibody. Western Blot analysis of DCPS expression in PC-12.)
Application Data (Detection limit for recombinant GST tagged DCPS is ~3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to DCPS on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to DCPS on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1.5ug/ml])
WB (Western Blot) (DCPS monoclonal antibody Western Blot analysis of DCPS expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (63.18kD).)
WB (Western Blot) (Western blot analysis of GSR over-expressed 293 cell line, cotransfected with GSR Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with GSR monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged GSR is ~0.03ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to GSR on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 6ug/ml].)
WB (Western Blot) (Western Blot analysis of GSR expression in transfected 293T cell line by GSR monoclonal antibody. Lane 1: GSR transfected lysate (56.3kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (GSR monoclonal antibody Western Blot analysis of GSR expression in IMR-32.)
WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)
WB (Western Blot) (Western blot analysis of NDUFS4 over-expressed 293 cell line, cotransfected with NDUFS4 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NDUFS4 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged NDUFS4 is ~0.3ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NDUFS4 on formalin-fixed paraffin-embedded human colon. [antibody concentration 1ug/ml])
WB (Western Blot) (Western Blot analysis of NDUFS4 expression in transfected 293T cell line by NDUFS4 monoclonal antibody. Lane 1: NDUFS4 transfected lysate (20.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (NDUFS4 monoclonal antibody Western Blot analysis of NDUFS4 expression in A-431.)
WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)
WB (Western Blot) (BACE1 monoclonal antibody (M02), clone 2C1. Western Blot analysis of BACE1 expression in rat testis.)
WB (Western Blot) (Western Blot analysis of BACE1 expression in transfected 293T cell line by BACE1 monoclonal antibody (M01), clone 2C1.Lane 1: BACE1 transfected lysate (52.9 KDa).Lane 2: Non-transfected lysate.)
Application Data (Proximity Ligation Analysis of protein-protein interactions between MMP2 and BACE1. HeLa cells were stained with anti-MMP2 rabbit purified polyclonal 1:1200 and anti-BACE1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
Application Data (Detection limit for recombinant GST tagged BACE1 is approximately 0.03ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to BACE1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to BACE1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml])
FCM (Flow Cytometry) (Dual staining of pig peripheral blood lymphocytes with Mouse anti Pig CD335 detected with Goat anti Mouse IgG (H/L):FITC (STAR117F), and Mouse anti Pig wCD8a:RPE)
Application Data (Published investigator image: Mouse anti Pig CD335 antibody, clone VIV-KM1 used for the identification of NK cells in influenza infected pigs by flow cytometry.Image caption: NK cell numbers in the blood of influenza infected pigs. Blood was taken from influenza A virus infected (n = 12) and control pigs (n = 9) on day 0-3 pi in a second experiment. (A) Isolated PBMCs were analysed by flow cytometry and live CD3- lymphocytes were gated as NKp46- or NKp46+ NK cells according to CD8a and NKp46 expression. Plots are taken from a representative control animal. Absolute numbers of (B) NKp46+ NK cells in PBMC were analysed by flow cytometry in control (left) and infected animals (right). (C) Results for the NKp46+ cells in the two groups were compared on each sampling day. (D) NKp46- NK cells in PBMC in control (left) and infected animals (right). (E) NKp46- NK cells were compared for the two groups. Each line in (B) and (D) represents one animal. **p=0.01.From: Forberg H, Hauge AG, Valheim M, Garcon F, Nunez A, et al. (2014) Early Responses of Natural Killer Cells in Pigs Experimentally Infected with 2009 Pandemic H1N1 Influenza A Virus.PLoS ONE 9(6): e100619.)
Application Data (Published investigator image: Mouse anti Pig CD335 antibody, clone VIV-KM1 used for the identification of NK cells in influenza infected pigs by immunofluorescence.Image caption:Staining for apoptosis in the lungs. Lung tissue sections from animals infected with influenza A virus (n = 12) were stained with immunofluorescence markers against (A) NKp46(green), (B) influenza A NP(red) and (C) the apoptosis marker caspase-3(blue). (D) Overlay displaying simultaneously influenza A virus NP+ and caspase-3+ cells as purple (arrows). Representative of virus infected bronchiole at day 1 pi. Immunofluorescence staining, 400x.From: Forberg H, Hauge AG, Valheim M, Garcon F, Nunez A, et al. (2014) Early Responses of Natural Killer Cells in Pigs Experimentally Infected with 2009 Pandemic H1N1 Influenza A Virus.PLoS ONE 9(6): e100619.)
Application Data (Published investigator image: Mouse anti Pig CD335 antibody, clone VIV-KM1 used for the identification of NK cells in influenza infected pigs by immunofluorescence.Image caption:NKp46+ cells in the lungs of influenza virus infected pigs.Lung tissue sections from pigs infected with influenza A virus and control pigs were stained with immunofluorescence markers for cytokeratin (blue), NKp46 (green) and influenza A virus NP (red). NKp46+ cells were counted in areas were influenza A virus NP was (A) detected and (B) not detected. Representative pictures taken from the same animal on day 1 pi are shown. Arrows point at NKp46+ cells. Immunofluorescence staining, 200x. (C) Plot shows number of NKp46+ cells per 0,1 mm2 in sections (n = 24 per animal) from control animals (n = 6) and in areas with and without virus in infected animals (n = 4 per day) calculated as described in Material and Methods. Groups with different letters differ significantly (p=0.05). (D) NKp46+ cells in the lumen of a bronchus (BL). Arrows point at the epithelial lining. Representative picture of luminal exudate, taken from an infected animal on day 2 pi. Insert shows NKp46+ and influenza A virus NP+ cell in the lung tissue of an infected animal on day 1 pi. Immunofluorescence staining, 400x.From: Forberg H, Hauge AG, Valheim M, Garcon F, Nunez A, et al. (2014) Early Responses of Natural Killer Cells in Pigs Experimentally Infected with 2009 Pandemic H1N1 Influenza A Virus.PLoS ONE 9(6): e100619.)
Application Data (Published investigator image: Mouse anti Pig CD335 antibody, clone VIV-KM1 used for the identification of NK cells in influenza infected pigs by flow cytometry.Image caption: Percentages of NK cells and expression of NKp46 and CD25 in lung tissue. Mononuclear cells were isolated from lung tissue of pigs infected with influenza A virus (n = 12) and control animals (n = 6) during the first 3 days pi and analysed by flow cytometry. (A) Live CD3- lymphocytes were gated as described in Fig 2. NK cells were gated according to CD8a and NKp46 expression and defined as NKp46-, NKp46int or NKp46high cells. Plot shown is from a representative control animal. (B) Proportions of NKp46- (green), NKp46int (blue) and NKp46high (purple) NK cells in individual animals, shown as percentages of gated cells in lymphocytes. (C) Percentages of NKp46- (left), NKp46int (middle) and NKp46high (right) NK cells among lymphocytes. (D) Median fluorescence intensity (MFI) in the NKp46- gate (left), the NKp46+ gate (middle) and in the NKp46high gate (right) are shown. (E) CD25+ cells were gated in the NKp46- and NKp46int NK cells (left) and in the NKp46high NK cells (right). Plots shown are from a representative control animal. (F) The percentages of CD25+ cells in each gate were calculated in control animals (green), infected animals from day 1 (purple), day 2 (blue) and day 3 (pink) pi. *p=0,05, **p=0.01.From: Forberg H, Hauge AG, Valheim M, Garcon F, Nunez A, et al. (2014) Early Responses of Natural Killer Cells in Pigs Experimentally Infected with 2009 Pandemic H1N1 Influenza A Virus.PLoS ONE 9(6): e100619.)
Application Data (Published investigator image: Mouse anti Pig CD335 antibody, clone VIV-KM1 used for the identification of NK cells in influenza infected pigs by immunofluorescence.Image caption:Counting of NKp46+ cells. Numbers of NKp46+ cells per area were counted in lung tissue sections from control animals and influenza A virus infected animals as described in Material and Methods. Representative picture of area with virus from infected animal. Immunofluorescence staining, 200x.From: Forberg H, Hauge AG, Valheim M, Garcon F, Nunez A, et al. (2014) Early Responses of Natural Killer Cells in Pigs Experimentally Infected with 2009 Pandemic H1N1 Influenza A Virus.PLoS ONE 9(6): e100619.)
Application Data (Published investigator image: Mouse anti Pig CD335 antibody, clone VIV-KM1 used for the evaluation of CD335 expression on porcine cells by flow cytometry.Image caption:Splenic NKp46high NK cells produced the highest levels of IFN-?. (A + B) Intracellular cytokine staining for IFN-? production in NKp46-defined NK cells within PBMC (upper graphs) and splenocytes (lower graphs) by four-colour FCM after 24 h in vitro stimulation with rhIL-2, rpIL-12 and rpIL-18 or medium. CD3- lymphocytes were gated (not shown) and NKp46/CD8a defined total NK cells were further subgated for IFN-? production. IFN-? producing NK cells were gated according to NKp46 expression levels (CD8a+NKp46-: blue, CD8a+NKp46+: green, CD8adim/-NKp46high: red). (A) Numbers indicate the percentage of IFN-?+ NK cells within respective gates. Results are representative for experiments with four different animals. (B) Percentage of IFN-?+ cells (left graph) and mean fluorescence intensity of IFN-?+ cells (right graph) within the different NK-cell subsets in blood and spleen of four animals are shown. (C) Supernatants of cytokine stimulated FACS-sorted CD3-CD8a+NKp46- and CD3-CD8a+NKp46+ NK cells from blood and CD3-CD8a+NKp46-, CD3-CD8a+NKp46+ and CD3-CD8adim/-NKp46high NK cells from spleen were tested for IFN-? production in ELISA following 24 h in vitro stimulation with rhIL-2, rpIL-12 and rpIL-18. Data on the left are from one representative animal and displayed as the mean of duplicates +/- SD. IFN-? production in experiments with four animals analysed is shown on the right. Mean values are represented by a black bar. (B + C) Significant differences between the subsets in blood or spleen are indicated (* = p < 0.05, ** = p < 0.01).From: Mair KH, Mllebner A, Essler SE, Duvigneau JC, Storset AK, Saalmller A, Gerner W. Porcine CD8adim/-NKp46high NK cells are in a highly activated state. Vet Res. 2013 Mar 1;44:13.)
Application Data (Published investigator image: Mouse anti Pig CD335 antibody, clone VIV-KM1 used for the evaluation of CD335 expression on porcine cells by flow cytometry.Image caption:Varying expression of NKp46, CD8a, CD16 and CD27 on NK-cell subsets in blood and spleen. (A) Following five-colour staining, PBMC and splenocytes were gated on CD3- cells and further subgated according to their NKp46/CD8a expression pattern in NKp46- and NKp46+ NK cells in blood (upper graph) and NKp46-, NKp46+ and NKp46high NK cells in spleen (lower graph). (B) 14 healthy 6 -7 month old pigs were investigated for NKp46 and CD8a expression levels in the NKp46-defined NK-cell subsets. Box-plots show the mean fluorescence intensity of the two markers. (C) NK-cell subsets defined in (A) were further analysed for their expression of the surface markers CD16 and CD27. Histograms show the expression of the two markers within the respective NKp46-defined subsets (CD8a+NKp46-: blue histograms, CD8a+NKp46+: green histograms, CD8adim/-NKp46high: red histograms) in blood (upper graphs) and spleen (lower graphs) according to the corresponding isotype control (grey histrograms with dotted lines). Box-plots show the mean fluorescence intensity of CD16 and CD27 of the NKp46-defined NK-cell subsets in blood and spleen of 14 healthy 6 -7 month old pigs. (B + C) Significant differences between the subsets in blood or spleen are indicated (* = p < 0.05, ** = p < 0.01, *** = p < 0.001).From: Mair KH, Mllebner A, Essler SE, Duvigneau JC, Storset AK, Saalmller A, Gerner W. Porcine CD8adim/-NKp46high NK cells are in a highly activated state. Vet Res. 2013 Mar 1;44:13.)
Application Data (Dual staining of pig peripheral blood lymphocytes with Mouse anti Pig CD335:Alexa Fluor488 and Mouse anti Pig wCD8a:RPE)
Application Data (Dual staining of pig peripheral blood lymphocytes with Mouse anti Pig CD335:APC and Mouse anti Pig wCD8a:RPE)
Application Data (Published customer image: Increased accumulation of repair-associated macrophages surrounding collaterals in ischemic hind limbs is PAR2-dependent. (A) Stainings of CD206-positive macrophages (green) and SMA-positive vessels (red) in non-ischemic (control) and ischemic (ligated) hind limbs of WT, PAR1-/- and PAR2-/- mice are shown. Nuclei were visualized with DAPI (blue). Arrows indicate single macrophages in the non-ischemic adductor. Quantification of the average number of repair-associated macrophages per vessel is indicated on the right. (B) Correlation between the number of CD206-positive macrophages in the ischemic tissues and the expression of CD11b and (C) CD115 on monocytes. ** p)
Application Data (Published customer image: Western blot analysis to determine type of activation of adult primary microglial cells produced from wild-type and IL-1 KO mice stimulated with IL-4, IL-13 or IL-4/IL-13 with or without IL-1beta. (A) Representative western blotting data of primary microglial cells produced from wild-type (wild) and IL-1 KO mice and exposed for 24 hours to IL-4, IL-13 or IL-4 plus IL-13 (IL-4/IL-13) with or without IL-1beta. Each lane expected to CD206 blotting were applied 7 ug of reduced samples. Non-reduced samples (5 ug) were applied to detect CD206. Densitometric analysis of COX2 (B), Ym1 (C), Arg-1 (D) and CD206 (E) (n = 3 each group). (B) COX2 levels are increased by exposure of cells to IL-1beta and are not influenced by IL-4 or IL-13 alone. The COX2 level was slightly enhanced by IL-1beta and IL-4 co-treatment. (C) Ym1 levels are increased by exposure of cells to IL-4 and IL-4/Il-13 and are synergistically increased by co-treatment with IL-1beta. However, only a low level of Ym1 is seen upon exposure of cells to IL-13, and is significantly less than that seen in response to exposure of cells to IL-4. (D) Arg-1 shows similar levels in response to exposure to IL-4 and IL-4/IL-13; these are synergistically increased by co-treatment with IL-1beta. However, low levels of Arg-1 are seen for exposure of cells to IL-13. (G) CD206 was detected in response to exposure of cells to both IL-4 and IL-4/IL-13 with or without IL-1beta; however, CD206 levels in IL-13-exposed samples were lower than those seen with the other treatments. Data are expressed as mean +/- SD (n = 3). *: P < 0.05, **: P < 0.01, ***: P < 0.001 compared with the IL-4-treated group without IL-1beta for each genotype (one-way ANOVA followed by Dunnett post-hoc test). ANOVA, analysis of variance; arg-1, arginase 1; COX 2, cyclooxygenase 2.From: Sato A, Ohtaki H, Tsumuraya T, Song D, Ohara K, Asano M, Iwakura Y, Atsumi T, Shioda S. Interleukin-1 participates in the classical and alternative activation of microglia/macrophages after spinal cord injury. J Neuroinflammation. 2012 Apr 7;9:65.)
Application Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained in blue using DAPI. Low power)
Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . High power)
Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Low power)
Application Data (Staining of J774 cell line with Rat anti Mouse CD206 following permeabilisation with Leucoperm)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD206: Alexa Fluor 488)
Application Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained in blue using DAPI. High power)
Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Medium power)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD206: Alexa Fluor 647)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD204: Alexa Fluor 488)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD204: Low Endotoxin)
Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD204 antibody, clone 2F8 followed by horseradish peroxidase Goat anti Rat IgG antibody . Low power)
Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD204 antibody, clone 2F8 followed by horseradish peroxidase Goat anti Rat IgG antibody . Medium power)
Application Data (Staining of mouse peritoneal macrophages cells with Rat anti Mouse CD204:FITC)
Application Data (Western Blot staining of J774 lysate (non reduced) probed with Rat anti Mouse CD204)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD204: Biotin)
Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD204 antibody, clone 2F8 followed by horseradish peroxidase Goat anti Rat IgG antibody . High power)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD204: Alexa Fluor 647)
Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD204:FITC)
Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . Medium power)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD314: Alexa Fluor 488)
Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . High power)
Application Data (Immunoperoxidase staining of a human spleen cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . high power)
Application Data (Immunoperoxidase staining of a human spleen cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . Low power)
Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD314 antibody, clone 1D11 followed by the Histar detection system . Low power)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD314)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD314:Low Endotoxin)
IHC (Immunohistchemistry) (TIGIT Antibody-Immunocytochemistry of TIGIT in over expressing HEK293 cells using TIGIT antibody and control mouse IgG antibody (left corner box) at 1 ug/ml.)
IHC (Immunohistochemistry) (TIGIT Antibody-Immunohistochemistry of TIGIT in human stomach carcinoma tissue using TIGIT Antibody and control mouse IgG (corner box) at 2 ug/ml.)
IHC (Immunohistochemistry) (TIGIT Antibody-Human Small Intestine, MALT: Formalin-Fixed, Paraffin-Embedded (FFPE) at 10 ug/ml.)
ICC (Immunocytochemistry) (TIGIT Antibody-Immunofluorescence of TIGIT in human stomach carcinoma tissue using TIGIT Antibody at 5 ug/ml. Green: TIGIT Antibody [3B5] Blue: DAPI staining)
ICC (Immunocytochemistry) (TIGIT Antibody-Immunofluorescence of TIGIT in over expressing HEK293 cells using TIGIT Antibody at 1 ug/ml. Green: TIGIT Antibody [3B5] Blue: DAPI staining)
FCM (Flow Cytometry) (TIGIT Antibody-Flow cytometry analysis of TIGIT over expressing HEK293 cells using TIGIT antibody at 1 ug/ml. Blue: untransfected HEK293 cells. Yellow: TIGIT over expressing HEK293 cells.)
WB (Western Blot) (RAB7B monoclonal antibody. Western Blot analysis of RAB7B expression in A-431.)
Application Data (Detection limit for recombinant GST tagged RAB7B is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of RAB7B transfected lysate using RAB7B monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with RAB7B rabbit polyclonal antibody.)
WB (Western Blot) (Western Blot analysis of RAB7B expression in transfected 293T cell line by RAB7B monoclonal antibody. Lane 1: RAB7B transfected lysate (22.5kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (RAB7B monoclonal antibody. Western Blot analysis of RAB7B expression in human kidney.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
WB (Western Blot) (Western Blot detection against Immunogen (41.62kD).)
Application Data (Detection limit for recombinant GST tagged C1D is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to C1D on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to C1D on formalin-fixed paraffin-embedded human colon. [antibody concentration 1ug/ml])
WB (Western Blot) (Western Blot analysis of C1D expression in transfected 293T cell line by C1D monoclonal antibody Lane 1: C1D transfected lysate (16kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (C1D monoclonal antibody Western Blot analysis of C1D expression in HeLa)
WB (Western Blot) (ERO1L monoclonal antibody. Western Blot analysis of ERO1L expression in PC-12.)
Application Data (Detection limit for recombinant GST tagged ERO1L is ~3ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ERO1L on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 1.5ug/ml].)
WB (Western Blot) (Western Blot analysis of ERO1L expression in transfected 293T cell line by ERO1L monoclonal antibody. Lane 1: ERO1L transfected lysate (54kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (ERO1L monoclonal antibody, Western Blot analysis of ERO1L expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (35.9kD).)
ICC (Immunocytochemistry) (ICC staining Histone H3.3 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Histone H3.3 in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-Histone H3.3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Histone H3.3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H3.3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Histone H3.3 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Histone H3.3 on BT-20 cell lysates using anti-Histone H3.3 antibody at 1/1, 000 dilution.)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with RAD18 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
ICC (Immunocytochemistry) (ICC staining RAD18 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining RAD18 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining RAD18 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-RAD18 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of RAD18 on different lysates using anti-RAD18 antibody at 1/1, 000 dilution. Positive control: Lane 1: NIH/3T3 Lane 2: 293T Lane 3: MCF-7)
FCM (Flow Cytometry) (Flow cytometric analysis of SH-SY-5Y cells with RACK1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-RACK1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-RACK1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-RACK1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat cerebellum tissue using anti-RACK1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of RACK1 on different lysates using anti-RACK1 antibody at 1/2, 000 dilution. Positive control: Lane 1: SH-SY-5Y Lane 2: Rat small intestine)
ICC (Immunocytochemistry) (ICC staining IGF2 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining IGF2 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining IGF2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining IGF2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-IGF2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-IGF2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-IGF2 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of IGF2 on human placenta lysates using anti-IGF2 antibody at 1/1, 000 dilution.)
FCM (Flow Cytometry) (Flow cytometric analysis of A549 cells with FGFR3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining FGFR3 in SH-SY5Y cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining FGFR3 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining FGFR3 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-FGFR3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-FGFR3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-FGFR3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-FGFR3 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of FGFR3 on different cells lysates using anti-FGFR3 antibody at 1/500 dilution. Positive control�� Line 1: 293 Line 2: HepG2)
FCM (Flow Cytometry) (Flow cytometric analysis of HepG2 cells with MTCO2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
ICC (Immunocytochemistry) (ICC staining MTCO2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining MTCO2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining MTCO2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MTCO2 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of MTCO2 on different lysates using anti-MTCO2 antibody at 1/1, 000 dilution. Positive control: Lane 1: MCF-7 Lane 2: THP-1 Lane 3: Hela)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with IKK gamma antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
ICC (Immunocytochemistry) (ICC staining IKK gamma in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-IKK gamma antibody. Counter stained with hematoxylin.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-IKK gamma antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse fallopian tube tissue using anti-IKK gamma antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-IKK gamma antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-IKK gamma antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-IKK gamma antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of IKK gamma on Hela cell using anti-IKK gamma antibody at 1/1, 000 dilution.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-HPRT antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-HPRT antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HPRT antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-HPRT antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of HPRT on Zebrafish tissue lysates using anti-HPRT antibody at 1/500 dilution.)
WB (Western Blot) (Western blot analysis of HPRT on different lysates using anti-HPRT antibody at 1/500 dilution. Positive control: Lane 1: Hela Lane 2: A431 Lane 3: MCF-7 Lane 4: 293 Lane 5: Rat kidney Lane 6: Rat brain Lane 7: Mouse testis Lane 8: Mouse colon)
FCM (Flow Cytometry) (Flow cytometric analysis of Jurkat cells with DIS3L2 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human bladder cancer tissue using anti-DIS3L2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human endometrial cancer tissue using anti-DIS3L2 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of DIS3L2 on Hela (1) and HepG2 (2) cell lysate using anti-DIS3L2 antibody at 1/1, 000 dilution.)
WB (Western Blot) (Western blot analysis of DIS3L2 on HEK293 (1) and DIS3L2-hIgGFc transfected HEK293 (2) cell lysate using anti-DIS3L2 antibody at 1/1, 000 dilution.)
WB (Western Blot) (Western blot analysis of DIS3L2 on human DIS3L2 recombinant protein using anti-DIS3L2 antibody at 1/1, 000 dilution.)
WB (Western Blot) (ACTN4 monoclonal antibody Western Blot analysis of ACTN4 expression in HeLa NE.)
WB (Western Blot) (ACTN4 monoclonal antibody Western Blot analysis of ACTN4 expression in NIH/3T3.)
Application Data (Detection limit for recombinant GST tagged ACTN4 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ACTN4 on HeLa cell. [antibody concentration 20ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ACTN4 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml].)
WB (Western Blot) (Western Blot analysis of ACTN4 expression in transfected 293T cell line by ACTN4 monoclonal antibody. Lane 1: ACTN4 transfected lysate (104.9kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (ACTN4 monoclonal antibody Western Blot analysis of ACTN4 expression in PC-12.)
IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using Vinculin Monoclonal Antibody at dilution of 1:200.)
IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using Vinculin Monoclonal Antibody at dilution of 1:200.)
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using Vinculin Monoclonal Antibody at dilution of 1:200.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human colon cancer using Vinculin Monoclonal Antibody at dilution of 1:1000.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human breast cancer using Vinculin Monoclonal Antibody at dilution of 1:1000.)
WB (Western Blot) (Western blot with Anti Vinculin Monoclonal Antibody at dilution of 1:3000. Lane 1: HeLa cell lysate, Lane 2: 293 cell lysate, Lane 3: Hep G2 cell lysate, Lane 4: NIH/3T3 cell lysate, Lane 5: Mouse heart tissue lysate, Lane 6: Rat heart tissue lysate.)
WB (Western Blot) (Western blot analysis of PITX1 over-expressed 293 cell line, cotransfected with PITX1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PITX1 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged PITX1 is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of PITX1 transfected lysate using PITX1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PITX1 rabbit polyclonal antibody.)
WB (Western Blot) (Western Blot analysis of PITX1 expression in transfected 293T cell line by PITX1 monoclonal antibody Lane 1: PITX1 transfected lysate (34.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (PITX1 monoclonal antibody Western Blot analysis of PITX1 expression in HeLa NE.)
WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)
WB (Western Blot) (GLMN monoclonal antibody Western Blot analysis of GLMN expression in HL-60.)
WB (Western Blot) (GLMN monoclonal antibody Western Blot analysis of GLMN expression in Jurkat.)
Application Data (Detection limit for recombinant GST tagged GLMN is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of GLMN transfected lysate using GLMN monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with GLMN rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to GLMN on HeLa cell. [antibody concentration 20ug/ml])
WB (Western Blot) (Western Blot analysis of GLMN expression in transfected 293T cell line by GLMN monoclonal antibody. Lane 1: GLMN transfected lysate (68.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (91.45kD).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TESK2 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TESK2 on HeLa cell. [antibody concentration 10 ug/ml])
Application Data (Detection limit for recombinant GST tagged TESK2 is approximately 0.1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TESK2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.7 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TESK2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.7 ug/ml])
WB (Western Blot) (Western Blot analysis of TESK2 expression in transfected 293T cell line by TESK2 monoclonal antibody (M08), clone 5G1.Lane 1: TESK2 transfected lysate(60.3 KDa).Lane 2: Non-transfected lysate.)
ICC (Immunocytochemistry) (ICC staining TIA1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining TIA1 in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining TIA1 in 293T cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-TIA1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-TIA1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of TIA1 on Jurkat cells lysates using anti-TIA1 antibody at 1/500 dilution.)
Application Data (Immunoperoxidase staining of human tonsil cryosection using Mouse ati Human CD21 antibody followed by HISTAR detection system. Medium power)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD21:FITC)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD21:RPE)
Application Data (Immunofluorescence staining of human tonsil cryosection using Mouse anti Human CD1a antibody , red in A and Mouse anti Human CD21, green in B. C is the merged image with nuclei counterstained blue using DAPI. High power)
Application Data (Immunoperoxidase staining of human tonsil cryosection using Mouse ati Human CD21 antibody followed by HISTAR detection system. Medium power)
Application Data (Immunofluorescence image of human tonsil cryosection stained with Sheep anti Human LOX-1 antibody in red and with Mouse anti Human CD21 antibody in red. Nuclei are stained blue using DAPI. Merged image on right)
Application Data (Immunofluorescence staining of human tonsil cryosection with Mouse anti Human CD11b antibody, clone ICRF44 , red in A and Mouse anti Human CD21, green in B. C is the merged image with nuclei counterstained blue using DAPI. medium power)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD21)
Application Data (Immunoperoxidase staining of human tonsil cryosection using Mouse ati Human CD21 antibody followed by HISTAR detection system. High power)
Application Data (Immunoperoxidase staining of human spleen cryosection using Mouse ati Human CD21 antibody followed by HISTAR detection system. High power)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded cerebellum tissues using ZEB1 mouse mAb with DAB staining.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using ZEB1 mouse mAb with DAB staining.)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells using CD22 mouse mAb (green) and negative control (red).)
IF (Immunofluorescence) (Immunofluorescence analysis of Hela cells using CD22 mouse mAb (green). Blue)
WB (Western Blot) (Western blot analysis using CD22 mouse mAb against L1210 (1), Hela (2), HEK293 (3), Jurkat (4), OCM-1 (5), A432 (6) and NIH/3T3 (7) cell lysate.)
WB (Western Blot) (Western blot analysis using CD22 mAb against HEK293 (1) and CD22 (AA)
WB (Western Blot) (Western blot analysis using CD22 mAb against human CD22 recombinant protein. (Expected MW is 37 kDa))
Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD86 antibody, clone 42F followed by horseradish peroxidase conjugated Goat anti Mouse IgG . Low power)
Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD86 antibody, clone 42F followed by horseradish peroxidase conjugated Goat anti Mouse IgG . Medium power)
Application Data (Staining of rat splenocytes with Mouse anti Rat CD86:RPE)
Application Data (Staining of rat splenocytes with Mouse anti Rat CD86:Alexa Fluor ?647)
Application Data (Staining of rat splenocytes with Mouse anti Rat CD86:Low Endotoxin)
Application Data (Staining of rat splenocytes with Mouse anti Rat CD86:FITC)
Application Data (Staining of rat splenocytes with Mouse anti Rat CD86:Alexa Fluor ?488)
Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD86 antibody, clone 42F followed by horseradish peroxidase conjugated Goat anti Mouse IgG . High power)
WB (Western Blot) (Western blot analysis of PITX1 over-expressed 293 cell line, cotransfected with PITX1 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PITX1 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged PITX1 is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of PITX1 transfected lysate using PITX1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PITX1 rabbit polyclonal antibody.)
WB (Western Blot) (Western Blot analysis of PITX1 expression in transfected 293T cell line by PITX1 monoclonal antibody Lane 1: PITX1 transfected lysate (34.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (PITX1 monoclonal antibody Western Blot analysis of PITX1 expression in HeLa NE.)
WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)
WB (Western Blot) (Western Blot analysis of IRF2 expression in transfected 293T cell line by IRF2 monoclonal antibody. Lane 1: IRF2 transfected lysate (39.4kD). Lane 2: Non-transfected lysate.)
Application Data (Detection limit for 128582 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence in HeLa cells using 128582 (10ug/ml).)
IHC (Immunohistochemistry) (Immunoperoxidase in formalin-fixed paraffin-embedded human leiomyosarcoma usign 128582 (3ug/ml).)
WB (Western Blot) (Western Blot analysis of IRF2 expression in Hela NE using 128582.)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RUNX2 on U-2 OS cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RUNX2 on U-2 OS cell. [antibody concentration 10 ug/ml])
Application Data (Detection limit for recombinant GST tagged RUNX2 is approximately 10ng/ml as a capture antibody.)
WB (Western Blot) (RUNX2 monoclonal antibody (M04), clone 4D5 Western Blot analysis of RUNX2 expression in PC-12 (Cat # L012V1).)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RUNX2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to RUNX2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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