Rabbit anti-Human Latexin Monoclonal Antibody | anti-LXN antibody

Recombinant Anti-Latexin Antibody, Rabbit Monoclonal

Cat. Num. AAA254707

• Gene Names: LXN; ECI; TCI
• Reactivity: Human
• Applications: Immunoprecipitation, Immunofluorescence, Immunocytochemistry, Flow Cytometry, Functional Assay, ELISA, Western Blot
• Purity: Protein A
• Synonyms: Latexin, Antibody; Recombinant Anti-Latexin Antibody, Rabbit Monoclonal; Latexin/LXN Antibody, Rabbit MAb; latexin; Anti-ECI Antibody; Anti-TCI Antibody; anti-LXN antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: Rabbit IgG
• Clone Number: 101
• Specificity: Human Latexin/LXN
• Purity/Purification: Protein A
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-LXN antibody: IP (Immunoprecipitation), IF (Immunofluorescence), ICC (Immunocytochemistry), FCM/FACS (Flow Cytometry), ELISA, WB (Western Blot)
• Antibody Type: Recombinant Antibody
• Immunogen: Recombinant Human Latexin/LXN Protein
• Conjugation: Unconjugated
• Preparation: This antibody was obtained from a rabbit immunized with purified, recombinant Human Latexin/LXN (rh Latexin/LXN) .
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

IP (Immunoprecipitation)

(LXN was immunoprecipitated using:Lane A:0.5 mg A549 Whole Cell LysateLane B:0.5 mg Hela Whole Cell Lysate2 uL anti-LXN rabbit monoclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-LXN rabbit monoclonal antibody,at 1:200 dilutionSecondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilutionDeveloped using the odssey technique.Performed under reducing conditions.Predicted band size: 25.7 kDaObserved band size: 25.7 kDa)

WB (Western Blot)

(Anti-LXN rabbit monoclonal antibody at 1:500 dilutionLane A: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.Predicted band size:26 kDaObserved band size:26 kDa)

FCM/FACS (Flow Cytometry)

(Flow cytometric analysis of Human LXN expression on MCF-7 cells. The cells were treated according to manufacturer's manual (BD Pharmingen'), stained with purified anti-Human LXN, then a FITC-conjugated second step antibody. The fluorescence histograms were derived from gated events with the forward and side light-scatter characteristics of intact cells.)

IF (Immunofluorescence)

(Immunofluorescence staining of Human LXN in MCF7 cells. Cells were fixed with 4% PFA, permeabilzed with 1% Triton X-100 in PBS, blocked with 10% serum, and incubated with Rabbit anti-Human LXN monoclonal antibody (1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor 488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.)

Related Product Information for anti-LXN antibody

Latexin, also known as endogenous carboxypeptidase inhibitor, tissue carboxypeptidase inhibitor, TCI, ECI, and LXN, is a cytoplasm protein that belongs to the protease inhibitor I47 (latexin) family. It is highly expressed in the heart, prostate, ovary, kidney, pancreas, and colon. Latexin/LXN is the only known endogenous specific inhibitor of zinc-dependent metallocarboxypeptidases (MCPs) present in mammalians so far. Latexin is originally identified as a molecular marker for the regional specification of the neocortex in development in rats. The 222 amino acid latexin in the human shows different expression distribution with high levels in heart, prostate, ovary, kidney, pancreas, and colon, but only moderate or low levels in other tissues including the brain. Latexin is also expressed at high levels and is inducible in macrophages in concert with other protease inhibitors and potential protease targets, and thus is suggested to play a role in inflammation and innate immunity pathways. Despite the non-detectable sequence similarity with plant and parasite inhibitors, Latexin is related to a human putative tumor suppressor protein, TIG1. Also, Latexin is implicated in Alzheimer's disease.

References

Liu Q., et al., (2000), Cloning, tissue expression pattern and genomic organization of latexin, a human homologue of rat carboxypeptidase A inhibitor. Mol. Biol. Rep. 27:241-246.Gauci S., et al., (2009), Lys-N and trypsin cover complementary parts of the phosphoproteome in a refined SCX-based approach.nal. Chem. 81:4493-4501.Muzny D.M., et al., (2006), The DNA sequence, annotation and analysis of human chromosome 3.Nature 440:1194-1198.

NCBI and Uniprot Product Information

• NCBI GI #: 194473714
• NCBI GeneID: 56925
• NCBI Accession #: NP_064554.3
• NCBI GenBank Nucleotide #: NM_020169.3
• UniProt Accession #: Q9BS40; Q96PN2; Q9NQS6
• Molecular Weight: 25,750 Da
• NCBI Official Full Name: latexin
• NCBI Official Synonym Full Names: latexin
• NCBI Official Symbol: LXN
• NCBI Official Synonym Symbols: ECI; TCI
• NCBI Protein Information: latexin; MUM; endogenous carboxypeptidase inhibitor; tissue carboxypeptidase inhibitor
• UniProt Protein Name: Latexin
• UniProt Gene Name: LXN
• UniProt Synonym Gene Names: ECI; TCI
• UniProt Entry Name: LXN_HUMAN