Active Proteins

AAA Biotech provides a variety of high-quality recombinant and natural/native proteins that are proven to work in a wide range of experiments. Explore our products to find the active protein that best fits your needs or experimental model.

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SDS-PAGE

Activity (IL1R1 (Interleukin 1 Receptor Type I), also known as CD121a, is an important mediator involved in many cytokine induced immune and inflammatory responses. It belongs to the interleukin-1 receptor family, and is a receptor for interleukin 1 alpha (IL1A), interleukin 1 beta (IL1B), and interleukin 1 receptor antagonist (IL1RA). Besides, mouse IL1B shares 87.0% AA sequence identity with rat IL1B, suggesting the exist of cross-species activity. Thus, a binding ELISA assay was constructed to detect the association of recombinant rat IL1R1 with recombinant mouse IL1B. Briefly, IL1R1 were diluted serially in PBS with 0.1%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL1B-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL1R1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL1R1 with IL1B was shown in Figure 1 and this effect was in a dose dependent manner.)

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Interleukin 1 Receptor Type I (IL1R1), Active Protein (Cat# AAA148235)

Full Name

Active Interleukin 1 Receptor Type I (IL1R1)

Reactivity

Rat

Applications

Activity Assay, Cell Culture

Purity

> 97%

Pricing

$215/0.01 mg $390/0.05 mg $590/0.1 mg $705/0.2 mg $1,335/0.5 mg $1,965/1 mg $2,600/2 mg $4,790/5 mg $9,335/2x5 mg $13,645/3x5 mg $17,715/4x5 mg $21,675/5x5 mg

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SDS-PAGE

Activity (Interleukin-6 (IL-6), a pro-inflammatory cytokine and an anti-inflammatory myokine, plays important roles in the acute phase reaction, inflammation, hematopoiesis, bone metabolism, and cancer progression. It has been reported that IL-6 significantly increased the MMP-10 protein lever in the human lung cancer A549 cells through the JAK/STAT signaling pathway. Briefly, A549 cells were seeded into 6-well cell culture clusters and allowed to grow to 50-70% confluence, then different concentrations of IL-6 was added. After incubated for 24h, the protein levels of MMP-10 in the cell supernatant were determined by Western blot.Result: MMP-10 protein lever significantly increased in A549 cells due to the stimulation of IL-6, the data was shown in Figure 1.)

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Interleukin 6 (IL6), Active Protein (Cat# AAA148238)

Full Name

Active Interleukin 6 (IL6)

Gene Names

Il6; ILg6; Ifnb2

Reactivity

Rat

Applications

Activity Assay, Cell Culture

Purity

> 95%

Pricing

$290/0.01 mg $575/0.05 mg $860/0.1 mg $1,065/0.2 mg $2,035/0.5 mg $3,020/1 mg $3,990/2 mg $7,365/5 mg $14,360/2x5 mg $20,985/3x5 mg $27,240/4x5 mg

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SDS-PAGE

Activity (Interleukin-17 receptor A (IL17RA) is a cytokine receptor which binds interleukin 17A (IL17A). IL17A is a proinflammatory cytokine secreted by activated T-lymphocytes. IL17RA, as a transmembrane protein, is a ubiquitous type I membrane glycoprotein that binds IL17A, IL17F and IL17C. Activation of IL17RA leads to induction of expression of inflammatory chemokines and cytokines such as CXCL1, CXCL8/IL8 and IL6. Thus a binding ELISA assay was constructed to detect the association of recombinant human IL17RA with recombinant human IL17A. Briefly, IL17RA were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL17A-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL17RA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL17RA with IL17A was shown in Figure 1 and this effect was in a dose dependent manner.)

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Interleukin 17 Receptor A (IL17RA), Active Protein (Cat# AAA148242)

Full Name

Active Interleukin 17 Receptor A (IL17RA)

Gene Names

IL17RA; CD217; IL17R; IMD51; CANDF5; CDw217; IL-17RA; hIL-17R

Reactivity

Human

Applications

Activity Assay, Cell Culture

Purity

> 90%

Pricing

$220/0.01 mg $410/0.05 mg $615/0.1 mg $750/0.2 mg $1,415/0.5 mg $2,100/1 mg $2,765/2 mg $5,110/5 mg $9,960/2x5 mg $14,550/3x5 mg $18,890/4x5 mg $23,100/5x5 mg

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SDS-PAGE

Activity (IL2RA (Interleukin-2 receptor alpha chain ), also known as Tac antigen and as CD25, was initially identified as a 55kDa membrane glycoprotein which is capable of binding IL2. Besides, mouse IL2 shares 75.1% AA sequence identity with rat IL2, suggesting the exist of cross-species activity. Thus we have conducted a binding ELISA assay to detect the interaction of recombinant rat IL2RA with recombinant mouse IL2. Briefly, IL2RA were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL2-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL2RA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL2RA with IL2 was shown in Figure 1 and this effect was in a dose dependent manner.)

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Interleukin 2 Receptor Alpha (IL2Ra), Active Protein (Cat# AAA148244)

Full Name

Active Interleukin 2 Receptor Alpha (IL2Ra)

Reactivity

Mouse

Applications

Activity Assay, Cell Culture

Purity

> 90%

Pricing

$220/0.01 mg $410/0.05 mg $615/0.1 mg $750/0.2 mg $1,415/0.5 mg $2,100/1 mg $2,765/2 mg $5,110/5 mg $9,960/2x5 mg $14,550/3x5 mg $18,890/4x5 mg $23,100/5x5 mg

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SDS-PAGE

Activity (IL17RD (interleukin-17 receptor D) is a membrane protein belonging to the IL17R protein family, acting as a component of the interleukin-17 receptor signaling complex. Knowing that the interaction between this protein and IL-17R does not require the interleukin, we have conducted a binding a binding ELISA assay to detect the interaction of recombinant human IL17RD with both recombinant human IL17RA and IL17. Briefly, IL17RD were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL17RA-coated or IL17-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL17RA pAb and anti-IL17 pAb separately, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution , wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL17RD with IL17RA and IL17 was shown in Figure 1 and Figure 2 respectively and this effect was in a dose dependent manner.)

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Interleukin 17 Receptor D (IL17RD), Active Protein (Cat# AAA148245)

Full Name

Active Interleukin 17 Receptor D (IL17RD)

Gene Names

IL17RD; SEF; HH18; IL-17RD; IL17RLM

Reactivity

Human

Applications

Activity Assay, Cell Culture

Purity

> 90%

Pricing

$335/0.01 mg $690/0.05 mg $1,070/0.1 mg $1,305/0.2 mg $2,540/0.5 mg $3,760/1 mg $4,990/2 mg $9,190/5 mg $17,910/2x5 mg $26,170/3x5 mg $33,980/4x5 mg

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SDS-PAGE

Activity (IL17RB (Interleukin-17 receptor B) is a receptor for the proinflammatory cytokines IL17B (Interleukin-17B) and IL17E (Interleukin-17E). This receptor has been shown to mediate the activation of NF-kappaB and the production of IL8 induced by IL17E. Thus a binding ELISA assay was constructed to detect the association of recombinant human IL17RB with recombinant human IL17B. Briefly, IL17RB were diluted serially in PBS, with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL17B-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IL17RB pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL17RB with IL17B was shown in Figure 1 and this effect was in a dose dependent manner.)

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Interleukin 17 Receptor B (IL17RB), Active Protein (Cat# AAA148246)

Full Name

Active Interleukin 17 Receptor B (IL17RB)

Gene Names

IL17RB; CRL4; EVI27; IL17BR; IL17RH1

Reactivity

Human

Applications

Activity Assay, Cell Culture

Purity

> 80%

Pricing

$215/0.01 mg $390/0.05 mg $590/0.1 mg $705/0.2 mg $1,335/0.5 mg $1,965/1 mg $2,600/2 mg $4,790/5 mg $9,335/2x5 mg $13,645/3x5 mg $17,715/4x5 mg $21,675/5x5 mg

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SDS-PAGE

Activity (NOG (Noggin) is a signaling molecule that plays an important role in promoting somite patterning in the developing embryo, essential for cartilage morphogenesis and joint formation. It is considered as an inhibitor in bone morphogenetic proteins (BMP) signaling, by binding with BMP4, thus a binding ELISA assay was conducted to detect the association of recombinant rat NOG with BMP4. Briefly, NOG were diluted serially in PBS with 0.01%BSA (pH 7.4). Duplicate samples of 100uL were then transferred to BMP4-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-NOG pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of NOG with BMP4 was shown in Figure 1 and this effect was in a dose dependent manner.)

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Noggin (NOG), Active Protein (Cat# AAA148248)

Full Name

Active Noggin (NOG)

Reactivity

Rat

Applications

Activity Assay, Cell Culture

Purity

> 90%

Pricing

$280/0.01 mg $540/0.05 mg $815/0.1 mg $990/0.2 mg $1,915/0.5 mg $2,830/1 mg $3,735/2 mg $6,885/5 mg $13,425/2x5 mg $19,615/3x5 mg $25,470/4x5 mg $31,190/5x5 mg

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SDS-PAGE

Activity (CA2 (Carbonic anhydrase 2) is an enzyme that catalyzes reversible hydration of carbon dioxide. It is essential for bone resorption and osteoclast differentiation and contributes to intracellular pH regulation in the duodenal upper villous epithelium during proton-coupled peptide absorption. It is widely accepted that CA2 also catalyzes hydrolysis of p-Nitrophenyl Acetate. Thus, a hydration assay was conducted to test the catalytic activity of CA2 using 4-Nitrophenyl Acetate (4-NPA) as substrate. Briefly, different concentrations of CA2 were incubated with 1mM 4-NPA in reaction buffer. The absorbance at the wavelength of 400nm was read per hour, and the result was shown in figure 1. It is obvious that CA2 catalyzes hydrolysis of p-Nitrophenyl Acetate.)

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Carbonic Anhydrase II (CA2), Active Protein (Cat# AAA148249)

Full Name

Active Carbonic Anhydrase II (CA2)

Gene Names

Car2; Ca2

Reactivity

Rat

Applications

Activity Assay, Cell Culture

Purity

> 97%

Pricing

$200/0.01 mg $335/0.05 mg $465/0.1 mg $575/0.2 mg $1,065/0.5 mg $1,545/1 mg $2,035/2 mg $3,745/5 mg $7,300/2x5 mg $10,665/3x5 mg $13,845/4x5 mg $16,920/5x5 mg

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Bioactivity (Interleukin 9, also known as IL-9, is a pleiotropic cytokine belonging to the group of interleukins. IL-9 secreted by CD4 helper cells that acts as a regulator of a variety of hematopoietic cells. This cytokine stimulates cell proliferation and prevents apoptosis. It functions through the interleukin-9 receptor (IL9R), which activates different signal transducer and activator (STAT) proteins namely STAT1, STAT3 and STAT5 and thus connects this cytokine to various biological processes. Besides, Interleukin 9 Receptor (IL9R) has been identified as an interactor of IL-9, thus a binding ELISA assay was conducted to detect the interaction of recombinant human IL-9 and recombinant human IL9R. Briefly, IL-9 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to IL9R-coated microtiter wells and incubated for 2h at 37. Wells were washed with PBST and incubated for 1h with anti-IL-9 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of IL-9 and IL9R was shown in Figure 1, and this effect was in a dose dependent manner.Figure. The binding activity of IL-9 with IL9R)

WB (Western Blot) (Figure. Western Blot)

SDS-PAGE (Figure. SDS-PAGE)

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Interleukin 9, Active Protein (Cat# AAA150065)

Full Name

Active Interleukin 9 (IL9)

Gene Names

IL9; P40; HP40; IL-9

Applications

Activity Assay, Cell Culture

Purity

>95%

Pricing

$320/0.01 mg $670/0.05 mg $1,015/0.1 mg $1,255/0.2 mg $2,440/0.5 mg $3,605/1 mg $4,780/2 mg $8,795/5 mg $17,150/2x5 mg $25,060/3x5 mg $32,535/4x5 mg

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Application Data (Tissue Factor Pathway Inhibitor 2 (TFPI2) takes part in the regulation of plasmin-mediated matrix remodeling. Inhibits trypsin, plasmin, factor VIIa/tissue factor and weakly factor Xa. TFPI2 doesn’t have any influence on thrombin. TFPI2 also can inhibit MMP activity, which can hydrolyze gelatin under certain conditions. Thus, the activity of TFPI2 can be measured by inhibit MMP-2 hydrolyze gelatin. Gelatin zymography is mainly used for the detection of the gelatinases, 2g/mL was denatured by SDS loading buffer, electrophoresed through sodium dodecylsulphate–polyacrylamide gel (SDS–PAGE; 8% gels) containing gelatin (1mg/mL) with nonreducing conditions. After renaturation, incubate with various concentrations of recombinant mouse TFPI2, then staining with coomassie brilliant blue G250, active MMP-2 would hydrolyze gelatin nearby, which was indicated by the white binds on the gel; if the activity of MMP-2 inhibit by TFPI2, there was none white binds on the gel.The result was shown in figure 1.As the figure 1 shown, MMP-2 can be inhibited by recombinant mouse TFPI2 at least 2.5g/mL.)

Gene Sequencing (Figure. Gene Sequencing (Extract))

WB (Western Blot) (Figure. Western Blot)

SDS-PAGE (Figure. SDS-PAGE)

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Tissue Factor Pathway Inhibitor 2, Active Protein (Cat# AAA150120)

Full Name

Active Tissue Factor Pathway Inhibitor 2 (TFPI2)

Applications

Activity Assay, Cell Culture

Purity

>95%

Pricing

$310/0.01 mg $650/0.05 mg $985/0.1 mg $1,215/0.2 mg $2,355/0.5 mg $3,465/1 mg $4,610/2 mg $8,455/5 mg $16,480/2x5 mg $24,080/3x5 mg $31,260/4x5 mg

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Bioactivity (Figure. The binding activity of SIRT3 with IDH2.)

WB (Western Blot) (Figure. Western Blot)

SDS-PAGE (Figure. SDS-PAGE)

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Sirtuin 3, Active Protein (Cat# AAA150138)

Full Name

Active Sirtuin 3 (SIRT3)

Gene Names

SIRT3; SIR2L3

Applications

Activity Assay, Cell Culture

Purity

>90%

Pricing

$275/0.01 mg $520/0.05 mg $780/0.1 mg $965/0.2 mg $1,850/0.5 mg $2,730/1 mg $3,595/2 mg $6,645/5 mg $12,945/2x5 mg $18,925/3x5 mg $24,565/4x5 mg $30,050/5x5 mg

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Bioactivity (Galectin 3 (GAL3) is a member of the lectin family, of which 14 mammalian galectins have been identified. It is also a member of the beta-galactoside-binding protein family that plays an important role in cell-cell adhesion, cell-matrix interactions, macrophage activation, angiogenesis, metastasis, apoptosis. The protein also has been demonstrated to be involved in cancer, inflammation and fibrosis, heart disease, and stroke. GAL3 is expressed in the nucleus, cytoplasm, mitochondrion, cell surface, and extracellular space. It also can agglutinate red blood. In this case, we chose rabbit erythrocyte (RaE) to assay its ability of agglutination. A general procedure for hemagglutination assay (or haemagglutination assay; HA) is as follows, two-fold dilute the recombinant mouse GAL3 with 0.9% sodium chloride injection, add 50uL a serial dilution of GAL3 to each well of a U or V-bottom shaped 96-well microtiter plate. The final well serves as a negative control without GAL3, replace with 50uL 0.9% sodium chloride injection. Then add 50uL 1% rabbit erythrocyte to each well and mixed gently. The plate is incubated for 3 hours at room temperature. The results are shown in Figure 1. It was obvious that the minimal effective concentration of GAL3 is 3.1ug/ml.)

SDS-PAGE (SDS-PAGE)

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Galectin 3 (GAL3), Active Protein (Cat# AAA152172)

Full Name

Active Galectin 3 (GAL3)

Gene Names

Lgals3; GBP; L-34; gal3; Mac-2

Applications

Activity Assay, Cell Culture

Purity

Greater than 97% by SDS-PAGE

Pricing

$300/0.01 mg $605/0.05 mg $925/0.1 mg $1,140/0.2 mg $2,195/0.5 mg $3,235/1 mg $4,300/2 mg $7,895/5 mg $15,395/2x5 mg $22,495/3x5 mg $29,205/4x5 mg

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Bioactivity (Extracellular matrix metalloproteinase (MMP) inducer (EMMPRIN), also known as basigin and CD147, is a 4466 kDa, variably N and Oglycosylated, type I transmembrane protein that belongs to the immunoglobulin superfamily. Human EMMPRIN is 269 amino acids (aa)

SDS-PAGE (Figure. SDS-PAGE)

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Cluster Of Differentiation 147 (CD147), Active Protein (Cat# AAA153101)

Full Name

Active Cluster Of Differentiation 147 (CD147)

Gene Names

Bsg; CE9; HT7; 5A11; Ox47R; EMMPRIN; basignin

Applications

Activity Assay, Cell Culture

Purity

Greater than 95% by SDS-PAGE

Pricing

$255/0.01 mg $495/0.05 mg $750/0.1 mg $915/0.2 mg $1,760/0.5 mg $2,600/1 mg $3,430/2 mg $6,365/5 mg $12,410/2x5 mg $18,140/3x5 mg $23,545/4x5 mg $28,810/5x5 mg

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SDS_PAGE (Sample: Active recombinant SOD1, Human)

Gene Sequencing (Gene Sequencing (extract))

Bioactivity (Superoxide Dismutase 1 (SOD1) is an enzyme that in human is encoded by the SOD1 gene. This gene encodes a member of the superoxide dismutase (SOD) protein family. SODs are antioxidant enzymes that catalyze the dismutation of two superoxide radicals into hydrogen peroxide and oxygen. Acroding to the report, in a weakly alkaline buffer solution (pH=8.2) with N-tris(hydroxymethyl)amino methane-HCL, pyrogallol can occur autoxidation in the air, then SOD can inhibit this reaction. Thus, we use this way to measued the activity of recombinant human SOD1. The reaction was performed in adding 7 ul 5 mmol/L pyrogallol to 200 ul 50 mmol/L Tris-HCl, rapidly mixing at 25 degree C, then read at 325 nm (using 50mmol/L Tris-HCl as blank control) in kinetic mode for 3 minutes using a microplate reader controlling the pyrogallol autoxidation rate at 0.70 OD/min. Different concentrations of recombinant human SOD1 were added into 200 ul 50 mmol/L Tris-HCl, incubated for 20 min at 25 degree C, then adding 7ul 5mmol/L pyrogallol to each well, rapidly mixing and read at 325 nm in kinetic mode for 3 minutes.Under these conditions, the enzyme amount of 50% inhibition of pyrogallol autooxidation per minute is defined as a unit. The specific activity of recombinant human SOD1 is 732 U/mg.)

Sequence

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Superoxide Dismutase 1 (SOD1), Active Protein (Cat# AAA153106)

Full Name

Active Superoxide Dismutase 1 (SOD1)

Gene Names

SOD1; ALS; SOD; ALS1; IPOA; hSod1; HEL-S-44; homodimer

Reactivity

Homo sapiens (Human)

Applications

Activity Assay, Cell Culture

Purity

>90%

Pricing

$275/0.01 mg $520/0.05 mg $780/0.1 mg $965/0.2 mg $1,850/0.5 mg $2,730/1 mg $3,595/2 mg $6,645/5 mg $12,945/2x5 mg $18,925/3x5 mg $24,565/4x5 mg $30,050/5x5 mg

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Bioactivity (17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3) belongs to the HSD17B family with NAD(P)H/NAD(P) -dependent oxidoreductase activity that catalyzes the interconversion between 17-ketosteroids and 17-hydroxysteroids to maintain the balance between les)

SDS-PAGE (Figure. SDS-PAGE)

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17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3), Active Protein (Cat# AAA153131)

Full Name

Active 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3)

Gene Names

HSD17B3; EDH17B3; SDR12C2

Applications

Activity Assay, Cell Culture

Purity

Greater than 90% by SDS-PAGE

Pricing

$285/0.01 mg $545/0.05 mg $830/0.1 mg $1,005/0.2 mg $1,945/0.5 mg $2,890/1 mg $3,790/2 mg $7,005/5 mg $13,660/2x5 mg $19,960/3x5 mg $25,910/4x5 mg

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Bioactivity (The interleukin-1 receptor antagonist (IL-1RA) is a member of the interleukin 1 cytokine family. IL1Ra is secreted by various types of cells including immune cells, epithelial cells, and adipocytes, and is a natural inhibitor of the pro-inflammatory effec)

SDS-PAGE (Figure. SDS-PAGE)

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Interleukin 1 Receptor Antagonist (IL1RA), Active Protein (Cat# AAA153008)

Full Name

Active Interleukin 1 Receptor Antagonist (IL1RA)

Gene Names

IL1RN; IRAP; IL-1RN; IL-1ra

Applications

Activity Assay, Cell Culture

Purity

Greater than 90% by SDS-PAGE

Pricing

$315/0.01 mg $660/0.05 mg $1,000/0.1 mg $1,240/0.2 mg $2,405/0.5 mg $3,540/1 mg $4,705/2 mg $8,650/5 mg $16,865/2x5 mg $24,645/3x5 mg $31,995/4x5 mg

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Bioactivity (Figure 2. The hemagglutination assay of GAL12 in V- bottom shaped 96-well microtiter plate.)

Bioactivity (Galectin-12 is a member of a family of mammalian lectins known as galectins. The galectins constitute a large family of carbohydrate-binding proteins that function in many systems both intracellularly and following secretion. Galectins contain either one)

SDS-PAGE (Figure. SDS-PAGE)

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Galectin 12 (GAL12), Active Protein (Cat# AAA153027)

Full Name

Active Galectin 12 (GAL12)

Applications

Activity Assay, Cell Culture

Purity

Greater than 90% by SDS-PAGE

Pricing

$280/0.01 mg $540/0.05 mg $820/0.1 mg $995/0.2 mg $1,925/0.5 mg $2,845/1 mg $3,750/2 mg $6,925/5 mg $13,495/2x5 mg $19,725/3x5 mg $25,605/4x5 mg

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Bioactivity (Lactotransferrin (LTF), also known as actoferrin (LF) , is a multifunctional protein of the transferrin family. Lactoferrin belongs to the innate immune system. Apart from its main biological function, namely binding and transport of iron ions, lactoferri)

SDS-PAGE (Figure. SDS-PAGE)

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Lactoferrin (LTF), Active Protein (Cat# AAA153058)

Full Name

Active Lactoferrin (LTF)

Gene Names

LTF; PLF

Applications

Activity Assay, Cell Culture

Purity

Greater than 90% by SDS-PAGE

Pricing

$285/0.01 mg $565/0.05 mg $845/0.1 mg $1,040/0.2 mg $1,995/0.5 mg $2,960/1 mg $3,900/2 mg $7,205/5 mg $14,045/2x5 mg $20,525/3x5 mg $26,645/4x5 mg

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Bioactivity (Programmed Cell Death 1 Ligand 2 (also known as PD-L2, B7-DC) is a member of the B7 family of proteins that provide signals for regulating T-cell activation and tolerance. Mature human PD-L2 consists of a 201 amino acid (aa) extracellular domain (ECD) wit)

SDS-PAGE (Figure. SDS-PAGE)

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Programmed Cell Death Protein 1 Ligand 2 (PDL2), Active Protein (Cat# AAA153059)

Full Name

Active Programmed Cell Death Protein 1 Ligand 2 (PDL2)

Gene Names

PDCD1LG2; B7DC; Btdc; PDL2; CD273; PD-L2; PDCD1L2; bA574F11.2

Applications

Activity Assay, Cell Culture

Purity

Greater than 95% by SDS-PAGE

Pricing

$220/0.01 mg $405/0.05 mg $605/0.1 mg $735/0.2 mg $1,390/0.5 mg $2,050/1 mg $2,720/2 mg $5,005/5 mg $9,755/2x5 mg $14,250/3x5 mg $18,500/4x5 mg $22,625/5x5 mg

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Bioactivity (Glypican 5 (GPC5) belongs to the glypican family of proteoglycans that are linked to the cell surface through a glycosyl-phosphatidylinositol anchor. GPC5 is expressed primarily in embryonic neurons and mesenchyme and it is implicated in a variety of physiological processes, ranging from cell proliferation to morphogenesis. Besides, Syndecan 1 (SDC1) has been identified as an interactor of GPC5, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant rat GPC5 and recombinant human SDC1. Briefly, GPC5 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to SDC1-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-GPC5 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant rat GPC5 and recombinant human SDC1 was shown in Figure 1, the EC50 for this effect is 0.6 ug/mL.)

SDS-PAGE (Figure. SDS-PAGE)

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Glypican 5 (GPC5), Active Protein (Cat# AAA161913)

Full Name

Active Glypican 5 (GPC5)

Applications

Activity Assay, Cell Culture

Purity

Greater than 90% by SDS-PAGE

Pricing

$200/0.01 mg $345/0.05 mg $490/0.1 mg $600/0.2 mg $1,125/0.5 mg $1,635/1 mg $2,160/2 mg $3,950/5 mg $7,695/2x5 mg $11,245/3x5 mg $14,595/4x5 mg $17,870/5x5 mg

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Bioactivity (Ephrin-A1 (EFNA1), also known as EPLG1, LERK1, TNFAIP4, Immediate early response protein B61, is a member of the A-type ephrin family of cell surface proteins that function as ligands for the A-type Eph receptor tyrosine kinase family. EFNA1 plays an important role in angiogenesis and tumor neovascularization. EFNA1 widely affects tumor growth through enhancing tumor angiogenesis, malignant cell events and invasiveness. Ephrin Type A Receptor 1 (EPHA1) is one of high-affinity ligands for EFNA1, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human EFNA1 and recombinant mouse EPHA1. Briefly, biotin-linked EFNA1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to EPHA1-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human EFNA1 and recombinant mouse EPHA1 was shown in Figure 1, the EC50 for this effect is 0.24 ug/mL.)

SDS-PAGE (Figure. SDS-PAGE)

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Ephrin A1 (EFNA1), Active Protein (Cat# AAA161914)

Full Name

Active Ephrin A1 (EFNA1)

Gene Names

EFNA1; B61; EFL1; ECKLG; EPLG1; LERK1; LERK-1; TNFAIP4

Applications

Activity Assay, Cell Culture

Purity

Greater than 95% by SDS-PAGE

Pricing

$220/0.01 mg $415/0.05 mg $620/0.1 mg $755/0.2 mg $1,440/0.5 mg $2,120/1 mg $2,805/2 mg $5,170/5 mg $10,080/2x5 mg $14,725/3x5 mg $19,115/4x5 mg $23,390/5x5 mg

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Bioactivity (Ephrin A2 (EFNA2) is a cell surface-associated protein belonging to the Ephrin family, which are involved in intercellular communication and cell localization. EFNA2 is mainly expressed in tissues including the brain, heart, lung, kidney and pancreas. And this protein is involved in regulating a variety of biological processes, including cell migration, cell differentiation, and tissue boundary formation, by interacting with Eph receptors. It aslo plays a significant role in the development and maintenance of the cardiovascular system, as well as in the regulation of synaptic plasticity and neuronal circuitry in the brain. A Disintegrin And Metalloprotease 10 (ADAM10), a disintegrin and metalloproteinase, can cleave EFNA2, potentially altering the activity of EFNA2 or its interaction with Eph receptors, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human EFNA2 and recombinant rat ADAM10. Briefly, biotin-linked EFNA2 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to ADAM10-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450 nm immediately. The binding activity of EFNA2 and ADAM10 was shown in Figure 1, the EC50 for this effect is 1.38 ug/mL.)

SDS-PAGE (Figure. SDS-PAGE)

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Ephrin A2 (EFNA2), Active Protein (Cat# AAA161915)

Full Name

Active Ephrin A2 (EFNA2)

Gene Names

EFNA2; ELF-1; EPLG6; LERK6; HEK7-L; LERK-6

Applications

Activity Assay, Cell Culture

Purity

Greater than 90% by SDS-PAGE

Pricing

$205/0.01 mg $360/0.05 mg $525/0.1 mg $645/0.2 mg $1,205/0.5 mg $1,760/1 mg $2,325/2 mg $4,260/5 mg $8,305/2x5 mg $12,130/3x5 mg $15,750/4x5 mg $19,295/5x5 mg

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Bioactivity (Ephrin-A4, also known as EFNA4 and EFL-4, is a member of the ligand of the EPH family. It is mainly expressed in the spleen, lymph nodes, ovary, small intestine and colon of adults, as well as in the heart, lungs, liver, and kidneys of the fetus. It is involved in the development of neurons, blood vessels, and epithelium by regulating cell migration, rejection, and adhesion. Ephrin-A4 has been shown to bind FYN,EphA2, EphA3, EphA4, EphA5, EphA6, EphA7, and EphB1. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant rat EFNA4 and recombinant human FYN. Briefly, EFNA4 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to FYN-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-EFNA4 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant rat EFNA4 and recombinant human FYN was shown in Figure 1, the EC50 for this effect is 0.37 ug/mL.)

SDS-PAGE (Figure. SDS-PAGE)

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Ephrin A4 (EFNA4), Active Protein (Cat# AAA161918)

Full Name

Active Ephrin A4 (EFNA4)

Applications

Activity Assay, Cell Culture

Purity

Greater than 90% by SDS-PAGE

Pricing

$285/0.01 mg $550/0.05 mg $835/0.1 mg $1,015/0.2 mg $1,965/0.5 mg $2,925/1 mg $3,850/2 mg $7,120/5 mg $13,880/2x5 mg $20,285/3x5 mg $26,335/4x5 mg

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Bioactivity (To test the effect of MIA1 on cell apoptosis, A375 cells were seeded into triplicate wells of 96-well plates at a density of 4,000 cells/well and allowed to attach overnight, then the medium was replaced with various concentrations of recombinant human MIA1 diluted with 5% serum standard DMEM. After incubated for 48h, cells were observed by inverted microscope and cell viability was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 ul of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Apoptosis of A375 cells after incubation with MIA1 for 48h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 assay after incubation with recombinant human MIA1 for 48h. The result was shown in Figure 2. It was obvious that MIA1 significantly decreased cell viability of A375 cells. The ED50 of recombinant human MIA1 is 0.53 ug/ml.)

Bioactivity (Melanoma inhibitory activity (MIA), also known as cartilage-derived retinoic acid-sensitive protein (CD-RAP), is a 12-kDa protein that is secreted from both chondrocytes and malignant melanoma cells. MIA has been reported to have effects on cell growth and adhesion, and it may play a role in melanoma metastasis and cartilage development. In the presence of MIA, we observed enhanced migratory ability of melanocytic cells, induction of melanoma-associated genes as well as inhibition of apoptosis due to anoikis. S100 Calcium Binding Protein B (S100B) is a high affinity ligand for MIA1, a functional binding ELISA assay was conducted to detect the interaction of recombinant human MIA1 and recombinant bovine S100B. Briefly, MIA1 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to S100B-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-MIA1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant humant MIA1 and recombinant bovine S100B was shown in Figure 1, the EC50 for this effect is 1.1 ug/mL.)

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Melanoma Inhibitory Activity Protein 1 (MIA1), Active Protein (Cat# AAA161926)

Full Name

Active Melanoma Inhibitory Activity Protein 1 (MIA1)

Gene Names

MIA; CD-RAP

Applications

Activity Assay, Cell Culture

Purity

Greater than 80% by SDS-PAGE

Pricing

$235/0.01 mg $440/0.05 mg $665/0.1 mg $820/0.2 mg $1,545/0.5 mg $2,280/1 mg $3,020/2 mg $5,580/5 mg $10,880/2x5 mg $15,900/3x5 mg $20,635/4x5 mg $25,245/5x5 mg

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Bioactivity (Cystatin D is a member of family 2 of the cystatin superfamily. In contrast to other members of family 2, Cystatin D has restricted tissue distribution and has been found only in saliva and tears. Two allelic variants (Arg46 and Cys46) are known in the human protein and they are not significantly different in their inhibitory activity against papain and cathepsins B, H, L and S. Recombinant Human Cystatin D corresponds to the Arg46 variant. The functions of Cystatin D are largely unknown. However, Cystatin D has been shown to inhibit coronavirus replication at its physiological concentration (0.121.9 uM) and has been suggested to play a protective role against proteases present in the oral cavity. The activity of recombinant human Cystatin D was measured by its ability to inhibit papain cleavage of a fluorogenic peptide substrate Z-FR-AMC in the assay buffer 50 mM Tris, pH 7.0. Papain was diluted to 500 ug/ml in activation buffer 50 mM Tris, 5 mM DTT, pH 7.0 and incubated at room temperature for 15 minutes. The activated papain was diluted to 100 ug/ml in the assay buffer and 20 ul different concentrations of recombinant human Cystatin D (MW: 17.45 KD) was incubated with 20 ul 100 ug/ml papain at 37 degree C for 10 minutes. Loading 50 uL of the incubated mixtures which were diluted five-fold in assay buffer into empty wells of a plate, and start the reaction by adding 50 uL of 200 uM substrate. Include a substrate blank containing 50 uL of assay buffer and 50 uL of 200 uM substrate. Then read at excitiation and emission wavelengths of 380 nm and 460 nm, respectively, in kinetic mode for 5 minutes. The result was shown in Figure 1 and it was obvious that recombinant human Cystatin D significantly decreased papain activity. The inhibition IC50 was )

SDS-PAGE (Figure. SDS-PAGE)

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Cystatin 5 (CST5), Active Protein (Cat# AAA161930)

Full Name

Active Cystatin 5 (CST5)

Applications

Activity Assay, Cell Culture

Purity

Greater than 95% by SDS-PAGE

Pricing

$235/0.01 mg $450/0.05 mg $680/0.1 mg $830/0.2 mg $1,580/0.5 mg $2,335/1 mg $3,085/2 mg $5,690/5 mg $11,090/2x5 mg $16,210/3x5 mg $21,040/4x5 mg $25,775/5x5 mg

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Sequence

Identification

SDS-PAGE

Bioactivity (Steroid 5 Alpha Reductase 1 (SRD5a1) a member of the steroid 5alpha-reductase family (SRD5A1, SRD5A2 and SRD5A3) converting testosterone into 5-alpha-dihydrotestosterone and progesterone or corticosterone into their corresponding 5-alpha-3-oxosteroids. It plays a central role in sexual differentiation and androgen physiology. SRD5A1 was mainly expressed in the skin, scalp, liver, and brain tissues. Prostatic Adenoma and Prostatic Hyperplasia are associated with SRD5A1. Besides, 17-Beta-Hydroxysteroid Dehydrogenase Type 3 (HSD17b3) has been identified as an interactor of SRD5a1, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse SRD5a1 and recombinant human HSD17b3. Briefly, biotin-linked SRD5a1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to HSD17b3-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant mouse SRD5a1 and recombinant human HSD17b3 was shown in Figure 1, the EC50 for this effect is 0.03 ug/mL.)

SDS-PAGE (Figure. SDS-PAGE)

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Steroid 5 Alpha Reductase 1 (SRD5a1), Active Protein (Cat# AAA161940)

Full Name

Active Steroid 5 Alpha Reductase 1 (SRD5a1)

Gene Names

Srd5a1; S5AR 1; Srd5a-1; 0610031P22Rik; 4930435F02Rik

Applications

Activity Assay

Purity

>80%

Pricing

$295/0.01 mg $605/0.05 mg $915/0.1 mg $1,135/0.2 mg $2,175/0.5 mg $3,230/1 mg $4,255/2 mg $7,865/5 mg $15,335/2x5 mg $22,405/3x5 mg $29,090/4x5 mg

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Bioactivity (Tryptase is a serine protease with trypsin-like activity, which is sometimes also referred to as Mast Cell Protease 7. It is stored in the secretory granules of mouse mast cells. It exhibits anticoagulant activity due to its ability to degrade fibrinogen in the presence of the diverse array of protease inhibitors in plasma. The activity of recombinant human TPS is measured by its ability to cleave a fluorogenic peptide substrate Mca-Arg-Pro-Lys-Pro-Val-Glu-Nval-Trp-Arg-Lys(Dnp)-NH2 in the assay buffer 50 mM Tris, pH 8.5. The rhTPS is diluted to 200 ug/ml in 50 mM Tris, 150 mM NaCl, 10 mM CaCl2, 0.05% (w/v) Brij-35, pH 7.5, then activated with 0.1 ug/ml Thermolysin at 37 degree C for 15min followed by adding 10 mM 1, 10 phenanthroline to stop activation. The activated rhTPS is diluted to 50 ug/mL in heparin incubation buffer of 100 ug/mL heparin, 50 mM MES, pH 5.5 and incubated at room temperature for 2 hours. Then the rhTPS was diluted to 12.5 ug/ml in assay buffer and load into a black well plate 50 uL and start the reaction by adding 50 uL of 20 uM substrate, with a substrate blank containing 50 uL assay buffer, 50 uL substrate, and no rhTPS. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The specific activity of recombinant human TPS is > 25 pmol/min/ug.)

SDS-PAGE (Figure. SDS-PAGE)

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Tryptase (TPS), Active Protein (Cat# AAA161828)

Full Name

Active Tryptase (TPS)

Gene Names

TPSAB1; TPS1; TPS2; TPSB1

Applications

Activity Assay, Cell Culture

Purity

Greater than 90% by SDS-PAGE

Pricing

$265/0.01 mg $510/0.05 mg $775/0.1 mg $940/0.2 mg $1,810/0.5 mg $2,685/1 mg $3,535/2 mg $6,530/5 mg $12,725/2x5 mg $18,595/3x5 mg $24,140/4x5 mg $29,580/5x5 mg

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Bioactivity (Complement Component 4b (C4b) is a component of the complement system, which is activated by the recognition of foreign pathogens, such as bacteria and viruses, or altered self-cells. In the classical activation pathway of complement, C4b is produced by the proteolytic cleavage of the precursor protein C4 by the activated enzyme C1s, and it can bind to C2a to form C3 invertase (C4b2a) which is responsible for the cleavage of C3. Besides, the binding of MASP2 to C4b is an important step in the lectin pathway of the complement system, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human C4b and recombinant human MASP2. Briefly, C4b was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to MASP2-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-C4b pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human C4b and recombinant human MASP2 was shown in Figure 1, the EC50 for this effect is 1.39 ug/mL.)

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Complement C4-B (C4B), Active Protein (Cat# AAA161839)

Full Name

Active Complement C4-B (C4B)

Gene Names

C4B; CH; C4F; CO4; C4B1; C4B2; C4B3; C4B5; C4BD; C4B12; C4B_2; CPAMD3

Applications

Activity Assay, Cell Culture

Purity

Greater than 90% by SDS-PAGE

Pricing

$275/0.01 mg $520/0.05 mg $780/0.1 mg $965/0.2 mg $1,850/0.5 mg $2,730/1 mg $3,595/2 mg $6,645/5 mg $12,945/2x5 mg $18,925/3x5 mg $24,565/4x5 mg $30,050/5x5 mg

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Bioactivity (Thymic stromal lymphopoietin (TSLP) is a member of the IL-2 cytokine family and a distant paralog of IL-7. TSLP is a pleiotropic cytokine that acts on multiple cell lineages, including dendritic cells, T cells, B cells, neutrophils, mast cells, eosinophils and innate lymphoid cells, affecting their maturation, survival and recruitment. It is best known for its role in promoting type 2 immune responses such as in allergic diseases. Interleukin 13 (IL13) is a critical downstream element for TSLP-driven allergic inflammation. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human TSLP and recombinant bovine IL13. Briefly, TSLP was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to IL13-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-TSLP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human TSLP and recombinant bovine IL13 was shown in Figure 1, the EC50 for this effect is 0.11 ug/mL.)

SDS-PAGE (Figure. SDS-PAGE)

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Thymic Stromal Lymphopoietin (TSLP), Active Protein (Cat# AAA161842)

Full Name

Active Thymic Stromal Lymphopoietin (TSLP)

Applications

Activity Assay, Cell Culture

Purity

Greater than 95% by SDS-PAGE

Pricing

$305/0.01 mg $620/0.05 mg $945/0.1 mg $1,170/0.2 mg $2,260/0.5 mg $3,345/1 mg $4,425/2 mg $8,145/5 mg $15,880/2x5 mg $23,205/3x5 mg $30,130/4x5 mg

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Bioactivity (Hemoglobin (HB) is a protein in red blood cells which contains iron. It is used to transport oxygen around the human body. Hemoglobin is found in the red blood cells of almost all vertebrates. Hemoglobin has peroxidase activity. In the presence of hydrogen peroxide, hemoglobin can catalyze the substrates 3,3 ', 5,5' - tetramethylbenzidine (TMB) to produce a blue compound which has a maximum absorption at 595 nm. Thus, the activity of native chinese hamster hemoglobin was measured by its ability to catalyze the substrates of TMB. The reaction was performed in PBS, pH 7.4 (Assay Buffer), ainitiated by addition 40 uL of various concentrations of HB (diluted by Assay Buffer) to 160 uL of TMB. Incubated at 37 degree C for 20min, then read at a wavelength of 595 nm immediately. The result was shown in figure 1, and the OD value was in a linear relationship with the concentration of HB.)

SDS-PAGE (Figure. SDS-PAGE)

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Hemoglobin (HB), Active Protein (Cat# AAA161849)

Full Name

Active Hemoglobin (HB)

Applications

Activity Assay, Cell Culture

Purity

Greater than 90% by SDS-PAGE

Pricing

$170/0.01 mg $285/0.05 mg $375/0.1 mg $450/0.2 mg $835/0.5 mg $1,215/1 mg $1,595/2 mg $2,920/5 mg $5,685/2x5 mg $8,305/3x5 mg $10,785/4x5 mg $13,190/5x5 mg

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Bioactivity (Complement Component 5 (C5), which forms part of the later stages of the complement pathway, is cleaved into C5a and C5b. C5a is a chemotactic agent for neutrophils, while C5b forms part of the complement membrane attack complex. Coagulation Factor II (F2) has been identified as an interactor of C5, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human C5 and recombinant mouse F2. Briefly, C5 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to F2-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-C5 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human C5 and recombinant mouse F2 was shown in Figure 1, the EC50 for this effect is 0.22 ug/mL.)

SDS-PAGE (Figure. SDS-PAGE)

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Complement Component 5 (C5), Active Protein (Cat# AAA161871)

Full Name

Active Complement Component 5 (C5)

Gene Names

C5; C5a; C5b; CPAMD4

Applications

Activity Assay, Cell Culture

Purity

Greater than 95% by SDS-PAGE

Pricing

$280/0.01 mg $535/0.05 mg $810/0.1 mg $985/0.2 mg $1,895/0.5 mg $2,815/1 mg $3,705/2 mg $6,840/5 mg $13,335/2x5 mg $19,485/3x5 mg $25,300/4x5 mg $31,000/5x5 mg

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Bioactivity (Protein Z-dependent Protease Inhibitor (ZPI), also known as SerpinA10 (SERine Proteinase INhibitor-clade A10) is a monomeric, secreted member of the A (or extracellular) clade within the serpin superfamily of protease inhibitors. In general, members of this superfamily regulate multiple proteolytic cascades, and are particularly effective due to the fact that their inhibitory activities can be fine-tuned through the participation of discrete, non-serpin co-factors. Serpins are unusual in that they are one-time use, non-recyclable proteins whose native state is thermodynamically unstable. The activity of recombinant human SERPINA10 was measured by its ability to inhibit Coagulation Factor X cleavage of a fluorogenic peptide substrate Mca-RPKPVE-Nval-WRK(Dnp)-NH2 in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. Coagulation Factor X was diluted to 5 ug/ml in the assay buffer and 25 ul different concentrations of recombinant human SERPINA10 (MW: 78.26 KD) was incubated with 25 ul diluted Coagulation Factor X at 37 degree C for 30 minutes. Loading 50 uL 20 uM substrate to start the reaction including a substrate blank containing 50 uL of assay buffer and 50 uL of 20 uM substrate. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The result was shown in Figure 1 and it was obvious that recombinant human SERPINA10 significantly decreased Coagulation Factor X activity. The inhibition IC50 was )

SDS-PAGE (Figure. SDS-PAGE)

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Serpin A10 (SERPINA10), Active Protein (Cat# AAA161873)

Full Name

Active Serpin A10 (SERPINA10)

Gene Names

SERPINA10; PZI; ZPI

Applications

Activity Assay, Cell Culture

Purity

Greater than 80% by SDS-PAGE

Pricing

$280/0.01 mg $525/0.05 mg $785/0.1 mg $970/0.2 mg $1,865/0.5 mg $2,750/1 mg $3,625/2 mg $6,695/5 mg $13,050/2x5 mg $19,070/3x5 mg $24,760/4x5 mg $30,335/5x5 mg

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Bioactivity (Fibroblast Growth Factor 12 (FGF12) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities, and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth, and invasion. Fibroblast Growth Factor 12 (FGF12) may represent an important modulator of neuronal network activity and has been associated with developmental and epileptic encephalopathy (DEE). Besides, it is reported that both domains of (Ca2)4-CaM directly bind two sites in the N-terminal domain (NTD) of A-type FGF splice variants (FGF11A, FGF12A, FGF13A, and FGF14A) with high affinity. Calmodulin 1 (CALM1) has been identified as an interactor of FGF12, thus a binding ELISA assay was conducted to detect the interaction of recombinant human FGF12 and recombinant human CALM1. Briefly, FGF12 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to CALM1-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-FGF12 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human FGF12 and recombinant human CALM1 was shown in Figure 1, the EC50 for this effect is 4.44 ug/mL.)

SDS-PAGE (Figure. SDS-PAGE)

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Fibroblast Growth Factor 12 (FGF12), Active Protein (Cat# AAA161897)

Full Name

Active Fibroblast Growth Factor 12 (FGF12)

Gene Names

FGF12; FHF1; FGF12B

Applications

Activity Assay, Cell Culture

Purity

Greater than 95% by SDS-PAGE

Pricing

$200/0.01 mg $340/0.05 mg $490/0.1 mg $600/0.2 mg $1,110/0.5 mg $1,625/1 mg $2,140/2 mg $3,930/5 mg $7,650/2x5 mg $11,180/3x5 mg $14,515/4x5 mg $17,775/5x5 mg

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Bioactivity (Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2  H2O = HCO3-  H, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. CA3 is a cytosolic enzyme with a very low CA activity. It is expressed at low levels in human muscle during early development but increases rapidly during the last trimester to reach 50-60% of adult levels at birth. The activity of recombinant human CA3 was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 12.5 mM Tris, 75 mM NaCl, pH 7.5 (assay buffer), initiated by addition 50 uL of various concentrations of CA3 (diluted by assay buffer) to 50 uL of 2 mM substrate 4-NPA (100 mM stock in Acetone, diluted by assay buffer). Incubated at 37 degree C for 5min, then read at a wavelength of 400 nm.)

SDS-PAGE (Figure. SDS-PAGE)

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Carbonic Anhydrase III, Muscle Specific (CA3), Active Protein (Cat# AAA161899)

Full Name

Active Carbonic Anhydrase III, Muscle Specific (CA3)

Gene Names

CA3; Car3; CAIII

Applications

Activity Assay, Cell Culture

Purity

Greater than 90% by SDS-PAGE

Pricing

$195/0.01 mg $320/0.05 mg $455/0.1 mg $555/0.2 mg $1,020/0.5 mg $1,495/1 mg $1,975/2 mg $3,630/5 mg $7,075/2x5 mg $10,340/3x5 mg $13,420/4x5 mg $16,445/5x5 mg

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Bioactivity (Tissue inhibitors of metalloproteinase 1 (TIMP1) is a member of the family of proteins that regulate the activation and proteolytic activity of the zinc enzymes known as matrix metalloproteinases (MMPs). TIMP-1 is a glycoprotein with a molecular mass of 28 kDa produced by a wide range of cell types. TIMP-1 inhibits active MMP-mediated proteolysis by forming an N-terminal, non-covalent binary complex with the MMP active site. The activity of recombinant dog TIMP1 was measured by its ability to inhibit rhMMP2 cleavage of a fluorogenic peptide substrate MCA-Pro-Leu-Gly-Leu-DPA-Ala-Arg-NH2 in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. rhMMP2 was diluted to 100 ug/ml and activated with 1 mM APMA at 37 degree C for 1 hour and TIMP1 (MW: 20.86 KD) was diluted to different concentrations with the assay buffer. Mix 8 ul of TIMP1 curve dilutions, 12.8 ul of activated rhMMP-2, and 59.2 ul of assay buffer, including a control containing assay buffer and the diluted rhMMP-2 and incubate the reactions for 2 hours at 37 degree C. Loading 50 ul of the incubated mixtures which were diluted five-fold in assay buffer into empty wells of a plate, and start the reaction by adding 50 ul of 20 uM substrate. Include a substrate blank containing 50 ul of assay buffer and 50 ul of 20 uM substrate. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The result was shown in Figure 1 and it was obvious that recombinant dog TIMP1 significantly decreased rhMMP2 activity. The inhibition IC50 was )

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Tissue Inhibitors Of Metalloproteinase 1 (TIMP1), Active Protein (Cat# AAA161742)

Full Name

Active Tissue Inhibitors Of Metalloproteinase 1 (TIMP1)

Gene Names

TIMP1; TIMP-1

Applications

Activity Assay, Cell Culture

Purity

Greater than 95% by SDS-PAGE

Pricing

$280/0.01 mg $535/0.05 mg $810/0.1 mg $985/0.2 mg $1,895/0.5 mg $2,815/1 mg $3,705/2 mg $6,840/5 mg $13,335/2x5 mg $19,485/3x5 mg $25,300/4x5 mg $31,000/5x5 mg

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Bioactivity (Matrix metalloproteinases are a family of zinc and calcium dependent endopeptidases with the combined ability to degrade all the components of the extracellular matrix. MMP-9 (gelatinase B) can degrade a broad range of substrates including gelatin, collagen types IV and V, elastin and proteoglycan core protein. It is believed to act synergistically with interstitial collagenase (MMP-1) in the degradation of fibrillar collagens as it degrades their denatured gelatin forms. MMP-9 is produced by keratinocytes, monocytes, macrophages and PMN leukocytes. MMP-9 is present in most cases of inflammatory responses. The activity of recombinant human MMP9 is measured by its ability to cleave a fluorogenic peptide substrate MCA-Pro-Leu-Gly-Leu-DPA-Ala-Arg-NH2 in the assay buffer 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, 0.05% (w/v) Brij-35, pH 7.5. The rhMMP9 is diluted to 100 ug/ml in assay buffer, then activated by p-aminophenylmercuric acetate (APMA) in a final concentration of 1 mM incubated at 37 degree C for 1 hours. The activated rhMMP9 is diluted to 0.2 ug/mL in assay buffer. Loading into a black well plate 50 uL of 0.2 ug/mL rhMMP9 and start the reaction by adding 50 uL of 20 uM substrate, with a substrate blank containing 50 uL assay buffer, 50 uL substrate, and no rhMMP9. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The specific activity of recombinant human MMP9 is > 3000 pmol/min/ug.)

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Matrix Metalloproteinase 9 (MMP9), Active Protein (Cat# AAA161746)

Full Name

Active Matrix Metalloproteinase 9 (MMP9)

Gene Names

MMP9; GELB; CLG4B; MMP-9; MANDP2

Applications

Activity Assay, Cell Culture

Purity

Greater than 95% by SDS-PAGE

Pricing

$315/0.01 mg $655/0.05 mg $995/0.1 mg $1,230/0.2 mg $2,380/0.5 mg $3,520/1 mg $4,665/2 mg $8,580/5 mg $16,730/2x5 mg $24,450/3x5 mg $31,745/4x5 mg

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Bioactivity (Homing Associated Cell Adhesion Molecule (HCAM), also known as CD44, is a ubiquitous multistructural and multifunctional cells surface adhesion molecule involved in cell-cell and cell-matrix interactions. CD44 is broadly expressed, including in the membranes of B cells, granulocytes, monocytes, and erythrocytes as well as on many thymocytes and mature T cells, besides it is highly expressed in many cancers and regulates metastasis via recruitment of CD44 to the cell surface. This protein is a receptor for hyaluronic acid (HA) and can also interact with other ligands, such as osteopontin, collagens, and matrix metalloproteinases (MMPs). Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse HCAM and biotinylated hyaluronan (HA). Briefly, biotin-linked HA was diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to HCAM-coated microtiter wells and incubated for 2h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 1 hour, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant mouse HCAM and biotinylated HA was shown in Figure 1, and this effect was in a dose dependent manner.)

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Homing Associated Cell Adhesion Molecule (HCAM), Active Protein (Cat# AAA161764)

Full Name

Active Homing Associated Cell Adhesion Molecule (HCAM)

Gene Names

Cd44; Ly-24; Pgp-1; HERMES; AU023126; AW121933; AW146109

Applications

Activity Assay, Cell Culture

Purity

Greater than 80% by SDS-PAGE

Pricing

$215/0.01 mg $375/0.05 mg $570/0.1 mg $680/0.2 mg $1,290/0.5 mg $1,885/1 mg $2,490/2 mg $4,575/5 mg $8,920/2x5 mg $13,040/3x5 mg $16,925/4x5 mg $20,725/5x5 mg

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Bioactivity (Tissue kallikreins are a family of extracellular serine proteases consisting of 15 members. Tissue kallikreins have attracted great interest as potential biomarkers for various cancers, including prostate, ovarian, breast, testicular, and lung. Human Kallikrein 6 (hKLK6) is a member of tissue kallikrein family observed in breast and brain tissues, colon carcinoma cells, and oligodedrocytes. Known protein substrates of hKLK6 are myelin basic protein, the precursor of the A beta amyloid peptide, and plasminogen. Its physiological functions may include the participation in demyelination processes as well as in the progression of inflammatory disease of the CNS. The activity assay of recombinant mouse KLK6 was measured by its ability to cleave the fluorogenic peptide substrate Boc-QAR-AMC. The rmKLK6 was activated by Lysyl-endopeptidase in the activation buffer 50 mM Tris, 0.05% (w/v) Brij-35, pH 8.0, of which equal volumes of 200 ug/ml rmKLK6 and 2.5 mU/ml Lysyl-endopeptidase were combined and incubated at room temperature for 30 minutes. The activated rmKLK6 was diluted to 3 ug/ml in assay buffer and start the reaction by adding 50 uL of 200 uM substrate. Read at excitation and emission wavelengths of 380 nm and 460 nm (top read), respectively, in kinetic mode for 5 minutes. The specific activity of recombinant mouse KLK6 is >6000 pmol/min/ug.)

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Kallikrein 6 (KLK6), Active Protein (Cat# AAA161769)

Full Name

Active Kallikrein 6 (KLK6)

Gene Names

Klk6; BSP; MSP; Bssp; Klk29; Prss9; Prss18; AI849898; neurosin

Applications

Activity Assay, Cell Culture

Purity

Greater than 90% by SDS-PAGE

Pricing

$280/0.01 mg $525/0.05 mg $785/0.1 mg $970/0.2 mg $1,865/0.5 mg $2,750/1 mg $3,625/2 mg $6,695/5 mg $13,050/2x5 mg $19,070/3x5 mg $24,760/4x5 mg $30,335/5x5 mg

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Bioactivity (C reactive protein (CRP) is an annular (ring-shaped), pentameric protein found in blood plasma, whose levels rise in response to inflammation. It is an acute-phase protein of hepatic origin that increases following interleukin-6 secretion by macrophages and T cells. Its physiological role is to bind to lysophosphatidylcholine expressed on the surface of dead or dying cells (and some types of bacteria) in order to activate the complement system via C1q. Besides, Coagulation Factor II (F2) has been identified as an interactor of CRP, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant dog CRP and recombinant rat F2. Briefly, CRP was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to F2-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CRP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant dog CRP and recombinant rat F2 was shown in Figure 1, the EC50 for this effect is 0.82 ug/mL.)

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C ReProtein (CRP), Active Protein (Cat# AAA161778)

Full Name

Active C Reactive Protein (CRP)

Applications

Activity Assay, Cell Culture

Purity

Greater than 90% by SDS-PAGE

Pricing

$280/0.01 mg $525/0.05 mg $805/0.1 mg $975/0.2 mg $1,875/0.5 mg $2,765/1 mg $3,660/2 mg $6,740/5 mg $13,140/2x5 mg $19,205/3x5 mg $24,930/4x5 mg $30,530/5x5 mg

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Bioactivity (PRL (prolactin), also known as luteotropin, is a hormone secreted from the pituitary gland and is best known for its role in enabling mammals to produce milk. PRL plays an essential role in metabolism, regulation of the immune system through activating its specific membrane-anchored receptor (PRLR). A functional ELISA assay was conducted to detect the interaction of recombinant bovine PRL and recombinant human PRLR. Briefly, PRL was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to PRLR-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-PRL pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant bovine PRL and recombinant human PRLR was shown in Figure 1, the EC50 for this effect is 0.05 ug/mL.)

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Prolactin (PRL), Active Protein (Cat# AAA161787)

Full Name

Active Prolactin (PRL)

Gene Names

PRL; GHA1; Prol

Applications

Activity Assay, Cell Culture

Purity

Greater than 90% by SDS-PAGE

Pricing

$290/0.01 mg $585/0.05 mg $870/0.1 mg $1,080/0.2 mg $2,065/0.5 mg $3,070/1 mg $4,035/2 mg $7,470/5 mg $14,565/2x5 mg $21,280/3x5 mg $27,625/4x5 mg

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Bioactivity (PRL (prolactin), also known as luteotropin, is a hormone secreted from the pituitary gland and is best known for its role in enabling mammals to produce milk. PRL plays an essential role in metabolism, regulation of the immune system through activating its specific membrane-anchored receptor (PRLR). A functional binding ELISA assay was conducted to detect the interaction of recombinant horse PRL and recombinant human PRLR. Briefly, PRL was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to PRLR-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-PRL pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant horse PRL and recombinant human PRLR was shown in Figure 1, the EC50 for this effect is 0.11 ug/mL.)

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Prolactin (PRL), Active Protein (Cat# AAA161789)

Full Name

Active Prolactin (PRL)

Gene Names

PRL; GHA1

Applications

Activity Assay, Cell Culture

Purity

Greater than 95% by SDS-PAGE

Pricing

$290/0.01 mg $575/0.05 mg $860/0.1 mg $1,065/0.2 mg $2,035/0.5 mg $3,020/1 mg $3,990/2 mg $7,365/5 mg $14,360/2x5 mg $20,985/3x5 mg $27,240/4x5 mg

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Bioactivity (Carbonic Anhydrase (CA) catalyzes the reversible reaction of CO2  H2O = HCO3-  H, which is fundamental to many processes such as respiration, renal tubular acidification and bone resorption. CA1 is a cytosolic enzyme with the highest levels in erythrocytes and is a very early marker for erythroid differentiation. The activity of recombinant human CA1 was measured by its ability to hydrolyze 4-Nitrophenyl acetate (4-NPA) to 4-Nitrophenol. The reaction was performed in 12.5 mM Tris, 75 mM NaCl, pH 7.5 (assay buffer), initiated by addition 50 uL of various concentrations of CA1 (diluted by assay buffer) to 50 uL of 2 mM substrate 4-NPA (100 mM stock in Acetone, diluted by assay buffer). Incubated at 37 degree C for 5min, then read at a wavelength of 400 nm.)

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Carbonic Anhydrase I (CA1), Active Protein (Cat# AAA161796)

Full Name

Active Carbonic Anhydrase I (CA1)

Gene Names

CA1; CAB; CA-I; Car1

Applications

Activity Assay, Cell Culture

Purity

Greater than 90% by SDS-PAGE

Pricing

$220/0.01 mg $410/0.05 mg $615/0.1 mg $750/0.2 mg $1,415/0.5 mg $2,100/1 mg $2,765/2 mg $5,110/5 mg $9,960/2x5 mg $14,550/3x5 mg $18,890/4x5 mg $23,100/5x5 mg

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Bioactivity (Peroxisome Proliferator Activated Receptor Gamma (PPARg) belongs to a large group of nuclear receptors controlling reproduction, metabolism, development and immune response. It is mainly expressed in adipose tissue, hematopoietic cells and the large intestine and it plays an important role in adipocyte differentiation, lipid and glucose metabolism, and modulation of immune and inflammatory reactions. Besides, Peroxisome Proliferator Activated Receptor Gamma Coactivator 1 Alpha (PPARgC1a) has been identified as an interactor of PPARg, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human PPARg and recombinant mouse PPARgC1a. Briefly, PPARg was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to PPARgC1a-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-PPARg pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human PPARg and recombinant mouse PPARgC1a was shown in Figure 1, the EC50 for this effect is 0.03 ug/mL.)

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Peroxisome Proliferator Activated Receptor Gamma (PPARg), Active Protein (Cat# AAA161802)

Full Name

Active Peroxisome Proliferator Activated Receptor Gamma (PPARg)

Gene Names

PPARG; GLM1; CIMT1; NR1C3; PPARG1; PPARG2; PPARgamma

Applications

Activity Assay, Cell Culture

Purity

Greater than 90% by SDS-PAGE

Pricing

$280/0.01 mg $545/0.05 mg $820/0.1 mg $1,000/0.2 mg $1,930/0.5 mg $2,850/1 mg $3,760/2 mg $6,955/5 mg $13,560/2x5 mg $19,810/3x5 mg $25,720/4x5 mg

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Bioactivity (Glypican-3 (GPC3), a 70 kDa protein, is a member of the glypican family that attaches to the cell surface by a glycosylphosphatidylinositol anchor, is specifically up-regulated in hepatocellular carcinoma (HCC) although rarely or not expressed in normal liver tissues, making it a perfect test and treatment target for HCC. GPC3 is also a negative transcriptional regulator and tumor suppressor that inhibits the growth of breast, ovary, and lung cancer cells. It is reported that GPC3 can form a complex with insulin-like growth factor 2 (IGF2), and might thereby modulate IGF2 action. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human GPC3 and recombinant rat IGF2. Briefly, GPC3 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to IGF2-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-GPC3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human GPC3 and recombinant rat IGF2 was shown in Figure 1, the EC50 for this effect is 0.08 ug/mL.)

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Glypican 3 (GPC3), Active Protein (Cat# AAA161814)

Full Name

Active Glypican 3 (GPC3)

Gene Names

GPC3; SGB; DGSX; MXR7; SDYS; SGBS; OCI-5; SGBS1; GTR2-2

Applications

Activity Assay, Cell Culture

Purity

Greater than 80% by SDS-PAGE

Pricing

$215/0.01 mg $375/0.05 mg $570/0.1 mg $680/0.2 mg $1,290/0.5 mg $1,885/1 mg $2,490/2 mg $4,575/5 mg $8,920/2x5 mg $13,040/3x5 mg $16,925/4x5 mg $20,725/5x5 mg

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Bioactivity (The cytochrome P450 enzyme CYP2E1 catalyzes the oxidative metabolism of many solvents and other small organic molecules. CYP2E1 is expressed in adult and fetal human liver in addition to extrahepatic tissues such as lung and placenta. Treatment of primary cultures of human hepatocytes with ethanol induces CYP2E1 protein, and this is consistent with the finding that hepatic CYP2E1 protein and mRNA levels are increased in individuals with alcoholism. Although only a few drugs (e.g., acetaminophen have been identified as substrates for CYP2E1, many low molecular weight procarcinogens are activated by this cytochrome P450 (P450). Chlorzoxazone 6-hydroxylation, N-nitrosodimethylamine N-demethylation and p-nitrophenol hydroxylation can be used to measure the catalytic activity of CYP2E1. Thus, the recombinant mouse CYP2E1 activity was measured by its ability to hydroxylate p-nitrophenol to p-nitrocatechol. The reaction was performed in 50 mM potassium phosphate, pH 7.4 (Assay Buffer), initiated by addition 20 uL of 500 ug/ml CYP2E1 to 10 uL of 5 mM substrate p-nitrophenol and 30 ul of 26 mM NADPH in a total volume of 500 ul. Incubated at 37 degree C for 30min, then read at a wavelength of 535 nm after acidification of the reaction mixture with trichloroacetic acid followed by neutralization using 2 M NaOH.)

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Cytochrome P450 2E1 (CYP2E1), Active Protein (Cat# AAA161819)

Full Name

Active Cytochrome P450 2E1 (CYP2E1)

Gene Names

Cyp2e1; Cyp2e

Applications

Activity Assay, Cell Culture

Purity

Greater than 95% by SDS-PAGE

Pricing

$290/0.01 mg $580/0.05 mg $870/0.1 mg $1,075/0.2 mg $2,060/0.5 mg $3,060/1 mg $4,030/2 mg $7,460/5 mg $14,545/2x5 mg $21,250/3x5 mg $27,590/4x5 mg

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Bioactivity (Figure 2. Inhibition of HepG2 cells proliferation after stimulated with recombinant rat BMP4)

Bioactivity (BMP4 (Bone morphogenetic protein 4) is a member of the bone morphogenetic protein family, which is involved in bone and cartilage development, specifically tooth and limb development and fracture repair. To test the effect of BMP4 on cell apoptosis, HepG2 cells were seeded into triplicate wells of 96-well plates at a density of 4,000 cells/well and allowed to attach overnight, then the medium was replaced with various concentrations of recombinant rat BMP4 diluted with 5% serum standard DMEM. After incubated for 72h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10 ul of CCK-8 solution was added to each well of the plate, then the absorbance at 450 nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37 degree C. Apoptosis of HepG2 cells after incubation with BMP4 for 72h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant rat BMP4 for 72h. The result was shown in Figure 2. It was obvious that BMP4 significantly decreased cell viability of HepG2 cells. The ED50 of recombinant rat BMP4 is 18.5 ug/ml.)

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Bone Morphogenetic Protein 4 (BMP4), Active Protein (Cat# AAA161655)

Full Name

Active Bone Morphogenetic Protein 4 (BMP4)

Applications

Activity Assay, Cell Culture

Purity

Greater than 90% by SDS-PAGE

Pricing

$310/0.01 mg $650/0.05 mg $985/0.1 mg $1,215/0.2 mg $2,355/0.5 mg $3,465/1 mg $4,610/2 mg $8,455/5 mg $16,480/2x5 mg $24,080/3x5 mg $31,260/4x5 mg

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Bioactivity (Fibroblast Growth Factor 9 (FGF9) is a member of the fibroblast growth factor (FGF) family. FGF family members possess broad mitogenic and cell survival activities and are involved in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF9 was isolated as a secreted factor that exhibits a growth-stimulating effect on cultured glial cells. In nervous system, this protein is produced mainly by neurons and may be important for glial cell development. FGF9 secreted by HSC is a candidate activating ligand for hepatocyte FGFR4 functions, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human FGF9 and recombinant human FGFR4. Briefly, biotin-linked FGF9 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to FGFR4-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30 min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450 nm immediately. The binding activity of FGF9 and FGFR4 was shown in Figure 1, the EC50 for this effect is 0.23 ug/mL.)

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Fibroblast Growth Factor 9 (FGF9), Active Protein (Cat# AAA161661)

Full Name

Active Fibroblast Growth Factor 9 (FGF9)

Gene Names

FGF9; GAF; FGF-9; SYNS3; HBFG-9; HBGF-9

Applications

Activity Assay, Cell Culture

Purity

Greater than 90% by SDS-PAGE

Pricing

$210/0.01 mg $370/0.05 mg $550/0.1 mg $670/0.2 mg $1,255/0.5 mg $1,855/1 mg $2,440/2 mg $4,485/5 mg $8,740/2x5 mg $12,770/3x5 mg $16,575/4x5 mg $20,300/5x5 mg

What Are Active Proteins?

Proteins are large molecules made up of long chains of amino acids.
They will typically fold into a very particular 3-dimensional shape/conformation, that is sometimes referred to as their “native” form, which allows them to work properly in the body. For the purposes of product categorization, AAA Biotech will typically refer to proteins purified from their original animal host as being “native” proteins (this is to signify their difference compared to their “recombinant” or “synthetic” protein counterparts).

If a protein successfully folds into the correct shape, it is will typically display high fidelity characteristics to its original protein in its original animal host, and be classified as an active protein, as it will be able to function “normally” in most enzymatic or binding capacities. If it loses this shape, due to factors such as heat or strong chemicals (such as detergents), it becomes inactive and is no longer able to perform its basic functions. All of the proteins in this category are made under strict quality control, and they are active, pure, low in contaminants, and stable.
Most are stored as freeze-dried powders and come without extra tags, so they’re very close to the actual natural/native form.

Key Applications of Active Proteins

1. Scientific Research

• Aid in the study of how proteins function in the body


• Aid in understanding various disease processes

2. Drug Development

• Powerful tools to investigate how potential drugs interact with specific proteins


• Ideal for identifying drug targets

3. Cell Culture

• Are routinely utilized to support cell growth and function (e.g., using exogenous growth factors)


• Can be used to promote cellular development into specific types (differentiation)

4. Diagnostics

• Regularly utilized in tests to detect diseases or infections (e.g., COVID-19, cancer)


• Note: All products are strictly for research-use only (RUO).

5. Therapeutics

• Some active proteins are used directly as treatments (e.g., insulin, enzymes)


• Note: All products are strictly for research-use only (RUO).

6. Vaccine Development

• Used to create or test vaccines by mimicking parts of viruses or bacteria

7. Biochemical Assays

• They can facilitate the characterization of enzyme activity, binding strength, or protein interactions in lab tests

Why Buy Active Proteins from AAA Biotech?

• High biological activity – Verified to perform as expected or indicated on datasheet


• Strict quality control – We are confident in our active proteins’ reliability and consistency


• High purity & low endotoxin – Ideal for applications involving sensitive or precious samples/components


• Freeze-dried for stability – Long shelf life and straightforward storage


• Mostly tag-free – Closer to natural/native protein form

FAQ

1. What are active proteins used for in research?

Active proteins are used primarily in the study of how proteins function, in characterizing/discovering drug interactions, supporting cell growth, running biochemical assays, and in development of diagnostics or therapeutics.

2. How are AAA Biotech's active proteins validated?

AAA Biotech’s active proteins are validated through strict quality control and functional assays to ensure they are properly folded and active. “Active”, though, can be an ambiguous term, so if a specific “activity” or “binding” capability of a protein is of crucial interest to you, please inquire with us prior to purchase, and we will provide further details on how the “Active” modifier was determined to be applicable.

3. Are these proteins tested for biological activity?

Yes, all active proteins from AAA Biotech are tested to confirm they have the expected biological activity before being offered for use. Though, said “biological activity” can be either “enzymatic”, “binding”, or both.