AAA Biotech provides a variety of high-quality recombinant and natural/native proteins that are proven to work in a wide range of experiments. Explore our products to find the active protein that best fits your needs or experimental model.
Bioactivity (Figure. The binding activity of PSA with PDIA3.Puromycin Sensitive Aminopeptidase (PSA) also known as cytosol alanyl aminopeptidase or alanine aminopeptidase (AAP) is used as a biomarker to detect damage to the kidneys, and that may be used to help idenfity certain kidney disorders. It is found at high levels in the urine when there are kidney problems. PSA has been proposed to function in a variety of processes, including metabolism of neuropeptidase, regulation of the cell cycle, and hydrolysis of proteasomal products to amino acids. Besides, Protein Disulfide Isomerase A3 (PDIA3) has been identified as an interactor of PSA, thus a binding ELISA assay was conducted to detect the interaction of recombinant human PSA and recombinant human PDIA3. Briefly, PSA were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to PDIA3-coated microtiter wells and incubated for 2h at 37. Wells were washed with PBST and incubated for 1h with anti-PSA pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of PSA and PDIA3 was shown in Figure 1, and this effect was in a dose dependent manner.)
SDS_PAGE (Figure 3. SDS-PAGESample: Active recombinant ANXA1, Mouse)
Application Data (Figure 2. Gene Sequencing (extract))
Bioactivity (Annexin A1 (ANXA1), also known as lipocortin I, belongs to the annexin family of Ca2+- dependent phospholipid-binding proteins that have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin A1 protein has an apparent relative molecular mass of 40kDa with phospholipase A2 inhibitory activity. Besides, S100 Calcium Binding Protein A11 (S100A11) has been identified as an interactor of ANXA1, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse ANXA1 and recombinant mouse S100A11. Briefly, ANXA1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to S100A11-coated microtiter wells and incubated for 2h at 37 degrees. Wells were washed with PBST and incubated for 1h with anti-ANXA1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degrees. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of ANXA1 and S100A11 was shown in Figure 1, and this effect was in a dose dependent manner.)
Bioactivity (Klotho (KL) is a transmembrane protein that, in addition to other effects, provides some control over the sensitivity of the organism to insulin and appears to be involved in aging. The Klotho protein is a novel -glucuronidase capable of hydrolyzing steroid -glucuronides. Genetic variants in KLOTHO have been associated with human aging, and Klotho protein has been shown to be a circulating factor detectable in serum that declines with age. The binding of certain fibroblast growth factors (FGFs) viz., FGF19, FGF20, and FGF23, to their fibroblast growth factor receptors, is promoted via their interactions as co-receptors with -Klotho. Besides, Fibroblast Growth Factor 23 (FGF23) has been identified as an interactor of KL, thus a binding ELISA assay was conducted to detect the interaction of recombinant rat KL and recombinant rat FGF23. Briefly, KL were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100L were then transferred to FGF23-coated microtiter wells and incubated for 2h at 37. Wells were washed with PBST and incubated for 1h with anti-KL pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of KL and FGF23 was shown in Figure 1, and this effect was in a dose dependent manner.Figure. The binding activity of KL with FGF23.)
HPLC (SARS COV-2 Spike S1 B.1.1.529 omicron HPLC. The purity of 2019-nCoV Spike B.1.1.529 omicron is greater than 95% as determined by SEC-HPLC.)
SDS-PAGE (S1 variant B.1.1.529 omicron protein PAGE. 1ug loaded Tris-Bis PAGE under reducing condition. The purity is greater than 95%.)
ELISA (SARS COV2 S1 B.1.1.529/omicron ACE functional binding ELISA assay. COV2 S1 (coating at 0.1ug-well) binding with Human ACE2-Fc .The linear range was found to be 100 ng/ml)
Bioactivity (Human IFN-alpha 2 (CAT# IFN0502) is coated at 10 ug/ml (100 ul/well) can bind Human IFN-alpha/beta R2 in the presence of Human IFN-alpha/beta R1 (CAT# ATGP3894). The ED50 range )
SDS-PAGE (3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
Application Data (CyaA accelerates cell detachment and migration of TLR-activated DCs.Migration of DCs treated with toxins and LPS (for 24 h) towards CCL19 or CCL21 (both 200 ng/ml) in transwell plates was determined by flow cytometry after additional 14 h (MDDCs) or 4 h (BMDCs) of incubation at 37°C.)
Application Data (Effect of hematopoietic S1P lyase deficiency on T-cell proliferation and migration.*P)
Application Data (Differential activation of MAP kinases in O. tsutsugamushi-infected DCs upon exposure to CCL19.DCs were stimulated with O. tsutsugamushi , or LPS for 18 h and then further incubated with CCL19 (200 ng/ml) for the indicated times.)
Application Data (Analysis of chemokine receptors and chemotaxis.The migration of MSCs to different concentrations of CXCL12 and CCL19 was evaluated by chemotaxis assay.)
Application Data (Effect of stem cell factor (SCF) on cardiac stem cell (CSC) migration in vitro.Representative images of migrated CSCs treated with varying concentrations of SCF by Transwell-based migration assays.)
Application Data (Pathological examination of livers and kidneys after SCF+G-CSF treatment during chronic stroke in aged mice.S+G 200/50: SCF (200 ?g/kg) in combination with G-CSF (50 ?g/kg).)
Application Data (Proliferation assay with the mouse hybridoma cell line B9. The cells were stimulated with increasing amounts of recombinant mouse IL-6.)
SDS-PAGE (SDS-PAGE analysis of recombinant mouse IL-6. Sample was loaded in 15% SDS-polyacrylamide gel under reducing conditions and stained with Coomassie blue.)
Application Data (Binding of Biotinylated VEGF165 to anti-human VEGF-A #3C5 in a functional ELISA. Anti-human VEGF-A antibody was coated with 0,5ug/ml (100ul/well) and Biotinylated VEGF165 was added starting with 3.125 ng/ml to 200 ng/ml.)
Application Data (Binding of Biotinylated VEGF165 to human soluble VEGFR-1/Flt-1 in a BioLISA. Recombinant human soluble VEGFR-1/Flt-1 was coated with 1ug/ml (100ul/well) over night at 4 degree C and Biotinylated VEGF165 was added.)
Application Data (Binding of Biotinylated VEGF165 to anti-human VEGF-A #3C5 in a functional ELISA. Anti-human VEGF-A antibody was coated with 0,5ug/ml (100ul/well) and Biotinylated VEGF165 was added starting with 3.125 ng/ml to 200 ng/ml.)
Application Data (Binding of Biotinylated VEGF165 to human soluble VEGFR-1/Flt-1 in a BioLISA. Recombinant human soluble VEGFR-1/Flt-1 was coated with 1ug/ml (100ul/well) over night at 4 degree C and Biotinylated VEGF165 was added.)
Proteins are large molecules made up of long chains of amino acids.
They will typically fold into a very particular 3-dimensional shape/conformation, that is sometimes referred to as their “native” form, which allows them to work properly in the body. For the purposes of product categorization, AAA Biotech will typically refer to proteins purified from their original animal host as being “native” proteins (this is to signify their difference compared to their “recombinant” or “synthetic” protein counterparts).
If a protein successfully folds into the correct shape, it is will typically display high fidelity characteristics to its original protein in its original animal host, and be classified as an active protein, as it will be able to function “normally” in most enzymatic or binding capacities. If it loses this shape, due to factors such as heat or strong chemicals (such as detergents), it becomes inactive and is no longer able to perform its basic functions. All of the proteins in this category are made under strict quality control, and they are active, pure, low in contaminants, and stable.
Most are stored as freeze-dried powders and come without extra tags, so they’re very close to the actual natural/native form.
Key Applications of Active Proteins
1. Scientific Research
Aid in the study of how proteins function in the body
Aid in understanding various disease processes
2. Drug Development
Powerful tools to investigate how potential drugs interact with specific proteins
Ideal for identifying drug targets
3. Cell Culture
Are routinely utilized to support cell growth and function (e.g., using exogenous growth factors)
Can be used to promote cellular development into specific types (differentiation)
4. Diagnostics
Regularly utilized in tests to detect diseases or infections (e.g., COVID-19, cancer)
Note: All products are strictly for research-use only (RUO).
5. Therapeutics
Some active proteins are used directly as treatments (e.g., insulin, enzymes)
Note: All products are strictly for research-use only (RUO).
6. Vaccine Development
Used to create or test vaccines by mimicking parts of viruses or bacteria
7. Biochemical Assays
They can facilitate the characterization of enzyme activity, binding strength, or protein interactions in lab tests
Why Buy Active Proteins from AAA Biotech?
High biological activity – Verified to perform as expected or indicated on datasheet
Strict quality control – We are confident in our active proteins’ reliability and consistency
High purity & low endotoxin – Ideal for applications involving sensitive or precious samples/components
Freeze-dried for stability – Long shelf life and straightforward storage
Mostly tag-free – Closer to natural/native protein form
FAQ
1. What are active proteins used for in research?
Active proteins are used primarily in the study of how proteins function, in characterizing/discovering drug interactions, supporting cell growth, running biochemical assays, and in development of diagnostics or therapeutics.
2. How are AAA Biotech's active proteins validated?
AAA Biotech’s active proteins are validated through strict quality control and functional assays to ensure they are properly folded and active. “Active”, though, can be an ambiguous term, so if a specific “activity” or “binding” capability of a protein is of crucial interest to you, please inquire with us prior to purchase, and we will provide further details on how the “Active” modifier was determined to be applicable.
3. Are these proteins tested for biological activity?
Yes, all active proteins from AAA Biotech are tested to confirm they have the expected biological activity before being offered for use. Though, said “biological activity” can be either “enzymatic”, “binding”, or both.
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