Bioactivity
(Human Thrombopoietin stimulates cell proliferation of the MO7e human megakaryocytic leukemic cells. The ED50 range )
Thrombopoietin Active Protein | THPO active protein
Recombinant human Thrombopoietin protein
Gene Names
THPO; ML; TPO; MGDF; MKCSF; MPLLG; THCYT1
Applications
SDS-Page
Purity
>90% by SDS-PAGE
Synonyms
Thrombopoietin; N/A; Recombinant human Thrombopoietin protein; THPO; MGDF; MKCSF; ML; MPLLG; THCYT1; TPO; thrombopoietin isoform 1 precursor; C-mpl ligand; Megakaryocyte colony-stimulating factor; Megakaryocyte growth and development factor; Myeloproliferative leukemia virus oncogene ligand; ATGP3057; THPO active protein
Host
HEK293
Purity/Purification
>90% by SDS-PAGE
Form/Format
Liquid. In Phosphate-Buffered Saline (pH7.4) containing 10% glycerol
Concentration
0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
Sequence Positions
22-353aa
Sequence
Applicable Applications for THPO active protein
Bioactivity, SDS-PAGE
Endotoxin
<1 EU per 1ug of protein (determined by LAL method)
Species
Human
Tag
His-Tag
Predicted N-term
Asp
Bioactivity
Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 range <=10ng/ml.
Preparation and Storage
Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.
Bioactivity
(Human Thrombopoietin stimulates cell proliferation of the MO7e human megakaryocytic leukemic cells. The ED50 range )
Bioactivity
(Human Thrombopoietin stimulates cell proliferation of the MO7e human megakaryocytic leukemic cells. The ED50 range )
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)
Related Product Information for THPO active protein
Thrombopoietin, also known as megakaryocyte growth and development factor (MGDF), is a member of EPO/TPO family. It is produced in the liver by both parenchymal cells and sinusoidal endothelial cells, as well as in the kidney by proximal convoluted tubule cells. It acts as a key regulator of megakaryocytopoiesis and thrombopoiesis that leads to platelet production. Also, this protein is the ligand for MLP/C_MPL, the product of myeloproliferative leukemia virus oncogene. Thrombopoietin, like EPO, plays a role in brain development. It promotes apoptosis of newly generated neurons, an effect counteracted by EPO and neurotrophins. Recombinant human Thrombopoietin fused to His-tag at C-terminus, was expressed in HEK293 cell and purified by using conventional chromatography techniques.
Product Categories/Family for THPO active protein
References
Fishley B, et al, (2004) Growth Factors. 22:151-155. Chou FS, et al, (2011) J Cell Biochem. 112:1491-1498. Kong Z, et al, (2017) Blood. 130:1097-1103.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33,604 Da
NCBI Official Full Name
thrombopoietin isoform 1
NCBI Official Synonym Full Names
thrombopoietin
NCBI Official Symbol
THPO
NCBI Official Synonym Symbols
ML; TPO; MGDF; MKCSF; MPLLG; THCYT1
NCBI Protein Information
thrombopoietin
UniProt Protein Name
Thrombopoietin
UniProt Gene Name
THPO
UniProt Synonym Gene Names
MGDF; ML; MGDF
UniProt Entry Name
TPO_HUMAN
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Product Notes
The THPO thpo (Catalog #AAA48378) is an Active Protein produced from HEK293 and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 22-353aa. AAA Biotech's Thrombopoietin can be used in a range of immunoassay formats including, but not limited to, Bioactivity, SDS-PAGE. Researchers should empirically determine the suitability of the THPO thpo for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below:Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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