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WB (Western Blot)
(Figure 4. Western BlotSample: Recombinant ANXA1, Mouse;Antibody: Rabbit Anti-Mouse ANXA1 Ab)
Annexin A1 Active Protein | ANXA1 active protein
Active Annexin A1 (ANXA1)
Gene Names
Anxa1; Lpc1; Anx-1; Lpc-1; Anx-A1; C430014K04Rik
Reactivity
Mus musculus (Mouse)
Applications
Activity Assay, Cell Culture
Purity
>92%
Synonyms
Annexin A1; N/A; Active Annexin A1 (ANXA1); ANX-A1; ANX1; LPC1; Lipocortin I; Chromobindin-9; Calpactin II; Phospholipase A2 inhibitory protein; ANXA1 active protein
Host
E. coli
Reactivity
Mus musculus (Mouse)
Purity/Purification
>92%
Form/Format
20mM Tris, 150mM NaCl, pH8.0, containing 0.01% skl and 5% trehalose.
Concentration
100ug/mL (varies by lot)
Applicable Applications for ANXA1 active protein
Activity Assay, Cell Culture
Source
Prokaryotic expression.
Residues
Glu6~Asn346
Tags
N-terminal His Tag
Endotoxin Level
<1.0EU per 1ug (determined by the LAL method).
Predicted Isoelectric Point
6.2
Predicted Molecular Mass
44.9kDa
Phenomenon Explanation
The possible reasons that the actual band size differs from the predicted are as follows:
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
1. Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleaved to give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
Usage
Reconstitute in ddH2O to a concentration of 0.1-0.5 mg/mL. Do not vortex.
Preparation and Storage
Avoid repeated freeze/thaw cycles.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
Store at 2-8 degree C for one month.
Aliquot and store at -80 degree C for 12 months.
Stability Test:
The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37 degree C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
WB (Western Blot)
(Figure 4. Western BlotSample: Recombinant ANXA1, Mouse;Antibody: Rabbit Anti-Mouse ANXA1 Ab)
WB (Western Blot)
(Figure 4. Western BlotSample: Recombinant ANXA1, Mouse;Antibody: Rabbit Anti-Mouse ANXA1 Ab)
SDS_PAGE
(Figure 3. SDS-PAGESample: Active recombinant ANXA1, Mouse)
SDS_PAGE
(Figure 3. SDS-PAGESample: Active recombinant ANXA1, Mouse)
Application Data
(Figure 2. Gene Sequencing (extract))
Application Data
(Figure 2. Gene Sequencing (extract))
Bioactivity
(Annexin A1 (ANXA1), also known as lipocortin I, belongs to the annexin family of Ca2+- dependent phospholipid-binding proteins that have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin A1 protein has an apparent relative molecular mass of 40kDa with phospholipase A2 inhibitory activity. Besides, S100 Calcium Binding Protein A11 (S100A11) has been identified as an interactor of ANXA1, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse ANXA1 and recombinant mouse S100A11. Briefly, ANXA1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to S100A11-coated microtiter wells and incubated for 2h at 37 degrees. Wells were washed with PBST and incubated for 1h with anti-ANXA1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degrees. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of ANXA1 and S100A11 was shown in Figure 1, and this effect was in a dose dependent manner.)
Bioactivity
(Annexin A1 (ANXA1), also known as lipocortin I, belongs to the annexin family of Ca2+- dependent phospholipid-binding proteins that have a molecular weight of approximately 35,000 to 40,000 and are preferentially located on the cytosolic face of the plasma membrane. Annexin A1 protein has an apparent relative molecular mass of 40kDa with phospholipase A2 inhibitory activity. Besides, S100 Calcium Binding Protein A11 (S100A11) has been identified as an interactor of ANXA1, thus a binding ELISA assay was conducted to detect the interaction of recombinant mouse ANXA1 and recombinant mouse S100A11. Briefly, ANXA1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100uL were then transferred to S100A11-coated microtiter wells and incubated for 2h at 37 degrees. Wells were washed with PBST and incubated for 1h with anti-ANXA1 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degrees. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of ANXA1 and S100A11 was shown in Figure 1, and this effect was in a dose dependent manner.)
Sequence
Sequence
Product Categories/Family for ANXA1 active protein
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
40kDa as determined by SDS-PAGE reducing conditions.
NCBI Official Full Name
annexin A1
NCBI Official Synonym Full Names
annexin A1
NCBI Official Symbol
Anxa1
NCBI Official Synonym Symbols
Lpc1; Anx-1; Lpc-1; Anx-A1; C430014K04Rik
NCBI Protein Information
annexin A1
UniProt Protein Name
Annexin A1
UniProt Gene Name
Anxa1
UniProt Synonym Gene Names
Anx1; Lpc-1; Lpc1
UniProt Entry Name
ANXA1_MOUSE
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Product Notes
The ANXA1 anxa1 (Catalog #AAA150137) is an Active Protein produced from E. coli and is intended for research purposes only. The product is available for immediate purchase. The Active Annexin A1 (ANXA1) reacts with Mus musculus (Mouse) and may cross-react with other species as described in the data sheet. AAA Biotech's Annexin A1 can be used in a range of immunoassay formats including, but not limited to, Activity Assay, Cell Culture. Researchers should empirically determine the suitability of the ANXA1 anxa1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Annexin A1, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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