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AAA Biotech also known as AAA Bio or AAABio provides a variety of high-quality recombinant and natural/native proteins that are proven to work in a wide range of experiments. Explore our products to find the active protein that best fits your needs or experimental model.
Viewing 800-850 of 2567 product results
Application Data
FGF8, Active Protein (Cat# AAA214334)
Full Name
Recombinant Mouse FGF8
Gene Names
FGF8; HH6; AIGF; KAL6; FGF-8; HBGF-8
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
CNTF, Active Protein (Cat# AAA214336)
Full Name
Recombinant Rat CNTF
Purity
>95% as determined by SDS-PAGE
Pricing
SDS-PAGE
Interleukin 2 (IL2), Active Protein (Cat# AAA148221)
Full Name
Active Interleukin 2 (IL2)
Gene Names
IL2; IL-2; TCGF; lymphokine
Reactivity
Human
Applications
Activity Assay, Cell Culture
Purity
> 95%
Pricing
Application Data
(Figure. Cell proliferation of Jurkat cells after stimulated with IL1a.Interleukin 1 alpha (IL1) also known as hematopoietin 1 is a cytokine of the interleukin 1 family that in humans is encoded by the IL1A gene. IL1 is produced mainly by activated macrophages, as well as neutrophils, epithelial cells, and endothelial cells. It possesses metabolic, physiological, haematopoietic activities, and plays one of the central roles in the regulation of the immune responses. It binds to the interleukin-1 receptor. It is on the pathway that activates tumor necrosis factor-alpha. To test the effect of IL1a on cell proliferation, Jurkat cells were seeded into triplicate wells of 96-well plates at a density of 2,000 cells/well with 2% serum standard 1640 including various concentrations of recombinant human IL1a. After incubated for 96h, cells were observed by inverted microscope and cell proliferation was measured by Cell Counting Kit-8 (CCK-8). Briefly, 10uL of CCK-8 solution was added to each well of the plate, then the absorbance at 450nm was measured using a microplate reader after incubating the plate for 1-4 hours at 37. Proliferation of Jurkat cells after incubation with IL1a for 96h observed by inverted microscope was shown in Figure 1. Cell viability was assessed by CCK-8 (Cell Counting Kit-8) assay after incubation with recombinant IL1a for 96h. The result was shown in Figure 2. It was obvious that IL1a significantly increased cell viability of Jurkat cells.(A) Jurkat cells cultured in 1640, stimulated with 10ng/mL IL1a for 96h; (B) Unstimulated Jurkat cells cultured in1640 for 96h.)
Interleukin 1 Alpha, Active Protein (Cat# AAA150058)
Full Name
Active Interleukin 1 Alpha (IL1a)
Gene Names
IL1A; IL1; IL-1A; IL1F1; IL1-ALPHA; IL-1 alpha
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
SDS_PAGE
(Sample: Active recombinant SOD3, Human)
Superoxide Dismutase 3, Extracellular, Active Protein (Cat# AAA150072)
Full Name
Active Superoxide Dismutase 3, Extracellular (SOD3)
Gene Names
SOD3; EC-SOD
Reactivity
Homo sapiens (Human)
Applications
Activity Assay, Cell Culture
Purity
>90%
Pricing
Application Data
(IL-1 (Interleukin-1 beta) is a proinflammatory and immunoregulatory cytokine involved in a variety of cellular activities. It has been elucidated that IL-1 stimulation of cells activates MMP-9 (matrix metalloproteinases-9) secretion by the activation of the dual signalling pathways. Thus, a stimulation assay of IL-1 was conducted using 3T3 cell line, and the MMP-9 activity was detected through gel zymography. Briefly, 1×106 3T3 cells were cultured overnight in 5%DMEM, washed with serum-free medium and then stimulated with different concentrations of IL-1 for 20h, and cell lysates were collected to measure MMP-9 activity. Cell lysates samples were denatured by SDS loading buffer, electrophoresed through sodium dodecyl sulphate–polyacrylamide gel (SDS–PAGE; 10% gels) containing gelatin (1mg/mL) with nonreducing conditions. After renaturation, incubation and coomassiebrilliant blue (CBB)-stained, MMPs hydrolyzed gelatin nearby, indicated by the white binds on the gel. Result Increased MMP-9 activity in 3T3 cells due to the stimulation of IL-1 was shown in Figure 1.Figure. Activation of MMP-9 by IL-1.)
Interleukin 1 Beta, Active Protein (Cat# AAA150093)
Full Name
Active Interleukin 1 Beta (IL1b)
Gene Names
Il1b; Il-1b; IL-1beta
Applications
Activity Assay, Cell Culture
Purity
>97%
Pricing
Application Data
(Figure. Cell proliferation of spleen cells after stimulated with GP39.)
Chitinase-3-like Protein 1, Active Protein (Cat# AAA150109)
Full Name
Active Chitinase-3-like Protein 1 (CHI3L1)
Gene Names
Chi3l1; Chil1; GP-39; CGP-39
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
Bioactivity
(Wingless-type MMTV integration site family, member 16 is a protein that in humans is encoded by the WNT16 gene. The WNT gene family consists of structurally related genes that encode secreted signaling proteins. These proteins have been implicated in oncogenesis and in several developmental processes, including regulation of cell fate and patterning during embryogenesis. This gene is a member of the WNT gene family. It contains two transcript variants diverging at the 5 termini. These two variants are proposed to be the products of separate promoters and not to be splice variants from a single promoter. Besides, Tubulin Beta 3 (TUBb3) has been identified as an interactor of WNT16, thus a binding ELISA assay was conducted to detect the interaction of recombinant human WNT16 and recombinant human TUBb3. Briefly, WNT16 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100L were then transferred to TUBb3-coated microtiter wells and incubated for 2h at 37. Wells were washed with PBST and incubated for 1h with anti-WNT16 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 3 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37. Finally, add 50uL stop solution to the wells and read at 450nm immediately. The binding activity of WNT16 and TUBb3 was shown in Figure 1, and this effect was in a dose dependent manner.Figure. The binding activity of WNT16 with TUBb3.)
Wingless Type MMTV Integration Site Family, Member 16, Active Protein (Cat# AAA150149)
Full Name
Active Wingless Type MMTV Integration Site Family, Member 16 (WNT16)
Applications
Activity Assay, Cell Culture
Purity
>95%
Pricing
Bioactivity
(FAS (Tumor necrosis factor receptor superfamily member 6) belongs to the tumor necrosis factor receptor superfamily. FAS contains a death domain, which has been shown to play a central role in the physiological regulation of programmed cell death. A bindi)
Factor Related Apoptosis (FAS), Active Protein (Cat# AAA152950)
Full Name
Active Factor Related Apoptosis (FAS)
Gene Names
FAS; APT1; CD95; FAS1; APO-1; FASTM; ALPS1A; TNFRSF6
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Cathepsin D is a lysosomal aspartic protease of the pepsin family. Human cathepsin D is synthesized as a precursor protein, consisting of a signal peptide (residues 118), a propeptide (residues 1964), and a mature chain (residues 65412). The mature chain can be processed further to the light (residues 65161) and heavy (residues 169412) chains. It is expressed in most cells and overexpressed in breast cancer cells. It is a major enzyme in protein degradation in lysosomes, and also involved in the presentation of antigenic peptides. Mice deficient in this enzyme showed a progressive atrophy of the intestinal mucosa, a massive destruction of lymphoid organs, and a profound neuronal ceroid lipofucinosis, indicating that cathepsin D is essential for proteolysis of proteins regulating cell growth and tissue homeostasis. The activity of recombinant human CTSD is measured by its ability to cleave a fluorogenic peptide substrate MCA-Pro-Leu-Gly-Leu-DPA-Ala-Arg-NH2 in the assay buffer 0.1 M NaOAc, 0.2 M NaCl, pH 3.5. The rhCTSD is diluted to 20 ug/ml in assay buffer, then incubated at 37 degree C for 30min. The incubated rhCTSD is diluted to 5 ug/mL in assay buffer. Loading into a black well plate 50 uL of 5 ug/mL rhCTSD and start the reaction by adding 50 uL of 60 uM substrate, with a substrate blank containing 50 uL assay buffer, 50 uL substrate, and no rhCTSD. Then read at excitiation and emission wavelengths of 320 nm and 405 nm, respectively, in kinetic mode for 5 minutes. The specific activity of recombinant human CTSD is > 675 pmol/min/ug.)
Cathepsin D (CTSD), Active Protein (Cat# AAA161838)
Full Name
Active Cathepsin D (CTSD)
Gene Names
CTSD; CPSD; CLN10
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Hemoglobin (HB) is a protein in red blood cells which contains iron. It is used to transport oxygen around the human body. Hemoglobin is found in the red blood cells of almost all vertebrates. Hemoglobin has peroxidase activity. In the presence of hydrogen peroxide, hemoglobin can catalyze the substrates 3,3 ', 5,5' - tetramethylbenzidine (TMB) to produce a blue compound which has a maximum absorption at 595 nm. Thus, the activity of native rabbit hemoglobin was measured by its ability to catalyze the substrates of TMB. The reaction was performed in PBS, pH 7.4 (Assay Buffer), ainitiated by addition 40 uL of various concentrations of HB (diluted by Assay Buffer) to 160 uL of TMB. Incubated at 37 degree C for 20min, then read at a wavelength of 595 nm immediately. The result was shown in figure 1, and the OD value was in a linear relationship with the concentration of HB.)
Hemoglobin (HB), Active Protein (Cat# AAA161850)
Full Name
Active Hemoglobin (HB)
Gene Names
HBA; HBA1
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Fatty acid binding protein-4 (FABP4) is a member of a large superfamily of lipid binding proteins that are expressed in a tissue specific manner. FABP4 is one of ten cytoplasmic FABPs that are 14-15 kDa in size and range from 126-140 amino acids (aa) in length. Although all are highly conserved in their tertiary structure, there is only modest aa identity between any two members. Lipase, Hormone Sensitive (LIPE) has been identified as an interactor of FABP4, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse FABP4 and recombinant human LIPE. Briefly, FABP4 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to LIPE-coated microtiter wells and incubated for 1h at 37 degree C. Wells were aspirated and incubated for 1h with anti-FABP4 mAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450 nm immediately. The binding activity of FABP4 and LIPE was shown in Figure 1, and this effect was in a dose dependent manner. The EC50 for this effect is 0.23 ug/mL.)
Fatty Acid Binding Protein 4 (FABP4), Active Protein (Cat# AAA161857)
Full Name
Active Fatty Acid Binding Protein 4 (FABP4)
Gene Names
Fabp4; Ap2; Lbpl; 422/aP2; ALBP/Ap2
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Cluster Of Differentiation 19 (CD19) is a type I transmembrane glycoprotein belonging to the immunoglobulin superfamily. It is expressed in normal and neoplastic B cells, and it modulates the threshold of B cell activation for amplifying B cell receptor signaling. This protein has recently emerged as a very attractive biomarker and therapeutic target for B-cell malignancies. The development of safe and effective ligands for CD19 has become an important need for the development of targeted conventional and immunotherapies. As far as we know, signaling through CD19 activates Vav/mitogen-activated protein kinase pathway and induces formation of a CD19/Vav/phosphatidylinositol 3-kinase complex in human B cell precursors. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human CD19 and recombinant human VAV1. Briefly, biotin-linked CD19 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to VAV1-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human CD19 and recombinant human VAV1 was shown in Figure 1, the EC50 for this effect is 0.67 ug/mL.)
Cluster Of Differentiation 19 (CD19), Active Protein (Cat# AAA161866)
Full Name
Active Cluster Of Differentiation 19 (CD19)
Gene Names
CD19; B4; CVID3
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(c-Met is a receptor tyrosine kinase belonging to the MET (MNNG HOS transforming gene) family, and is expressed on the surfaces of various cells. Hepatocyte growth factor (HGF) is the ligand for this receptor. The binding of HGF to c-Met initiates a series of intracellular signals that mediate embryogenesis and wound healing in normal cells. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human MET and recombinant human HGF. Briefly, MET was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to HGF-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-MET pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant human MET and recombinant human HGF was shown in Figure 1, the EC50 for this effect is 0.02 ug/mL.)
C-Met (MET), Active Protein (Cat# AAA161879)
Full Name
Active C-Met (MET)
Gene Names
MET; HGFR; AUTS9; RCCP2; c-Met
Applications
Activity Assay, Cell Culture
Purity
Greater than 80% by SDS-PAGE
Pricing
Bioactivity
(Transforming growth factor beta receptor 2 (TGFBR2) is a tumor suppressor gene that plays a role in the differentiation of striated cells and remodeling of coronary arteries. Single nucleotide polymorphisms (SNPs) of this gene are associated with Marfan syndrome and sudden death in patients with coronary artery disease. Cardiovascular remodeling and T cell activation of TGFBR2 gene suggest that the TGFBR2 gene SNPs are related to the pathogenesis of Kawasaki disease (KD) and coronary artery lesion (CAL). Endoglin can regulate endothelial cell shape changes in response to blood flow, which drive vascular remodeling and establishment of normal vascular morphology during angiogenesis. A functional ELISA assay was conducted to detect the interaction of recombinant human TGFBR2 and recombinant rat Endoglin (ENG). Briefly, TGFBR2 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to ENG-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-TGFBR2 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630nm immediately. The binding activity of recombinant human TGFBR2 and recombinant rat ENG was shown in Figure 1, the EC50 for this effect is 36.1 ng/mL.)
Transforming Growth Factor Beta Receptor II (TGFbR2), Active Protein (Cat# AAA161898)
Full Name
Active Transforming Growth Factor Beta Receptor II (TGFbR2)
Gene Names
TGFBR2; AAT3; FAA3; MFS2; RIIC; LDS1B; LDS2B; TAAD2; TGFR-2; TGFbeta-RII
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Glutathione S-Transferases (GSTs) are members of the phase II detoxification enzyme family that conjugate glutathione to various electrophilic compounds, including metabolites generated by oxidative processes in the body, environmental toxins or carcinogens, and anti-cancer drugs. GSTM1 is a cytosolic protein that belongs to the mu class of the GST superfamily. GSTM1 catalyze the endogenous glutathione conjugation 1-Chloro-2,4-dinitrobenzene (CDNB), which can increase in the absorbance at 340 nm. The reaction was performed in adding 10 ul 200 mM glutathione (reduced) and 10 ul 100 mM CDNB in 980 ul 100 mM NaH2PO4 (pH7.0), rapidly mixed. Then add 50 ul mixed substrates to 50 ul different concentrations of recombinant chicken GSTM1, mix gently. Incubated at 37 degree C for 5min, then read at a wavelength of 340 nm. The specific activity of recombinant chicken GSTM1 is >2500 pmol/min/ug.)
Glutathione S Transferase Mu 1 (GSTM1), Active Protein (Cat# AAA161759)
Full Name
Active Glutathione S Transferase Mu 1 (GSTM1)
Applications
Activity Assay, Cell Culture
Purity
Greater than 97% by SDS-PAGE
Pricing
Bioactivity
(GSTa3 (Glutathione S-transferase a3) is an enzyme that plays an important role in detoxification by catalyzing the conjugation of many hydrophobic and electrophilic compounds with reduced glutathione. This subfamily of enzymes has a particular role in protecting cells from Reactive Oxygen Species and the products of peroxidation. Polymorphisms in this gene influence the ability of individuals to metabolize different drugs. GSTa3 catalyze the endogenous glutathione conjugation 1-Chloro-2,4-dinitrobenzene (CDNB), which can increase in the absorbance at 340 nm. The reaction was performed in adding 10 ul 200 mM glutathione (reduced) and 10 ul 100 mM CDNB in 980 ul 100 mM NaH2PO4 (pH7.0), rapidly mixed. Then add 50 ul mixed substrates to 50 ul different concentrations of recombinant mouse GSTa3, mix gently. Incubated at 37 degree C for 5min, then read at a wavelength of 340 nm. The specific activity of recombinant mouse GSTa3 is >60,000 pmol/min/ug.)
Glutathione S Transferase Alpha 3 (GSTa3), Active Protein (Cat# AAA161767)
Full Name
Active Glutathione S Transferase Alpha 3 (GSTa3)
Gene Names
Gsta3; Gst2-3
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Reactive protein (CRP) is an annular (ring-shaped), pentameric protein found in blood plasma, whose levels rise in response to inflammation. It is an acute-phase protein of hepatic origin that increases following interleukin-6 secretion by macrophages and T cells. Its physiological role is to bind to lysophosphatidylcholine expressed on the surface of dead or dying cells (and some types of bacteria) in order to activate the complement system via C1q. Besides, Coagulation Factor II (F2) has been identified as an interactor of CRP, thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse CRP and recombinant rat F2. Briefly, CRP was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to F2-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-CRP pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant mouse CRP and recombinant rat F2 was shown in Figure 1, the EC50 for this effect is 0.02 ug/mL.)
C ReProtein (CRP), Active Protein (Cat# AAA161783)
Full Name
Active C Reactive Protein (CRP)
Gene Names
Crp; AI255847
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Retinol-binding protein 4 (RBP4) is the specific carrier for retinol (also known as vitamin A), and is responsible for the conversion of unstable and insoluble retinol in aqueous solution into stable and soluble complex in plasma through their tight interaction. As a member of the lipocalin superfamily, RBP4 containing a beta-barrel structure with a well-defined cavity is secreted from the liver, and in turn delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP4-retinol complex interacts with transthyretin (TTR), and this binding is crucial for preventing RBP4 excretion through the kidney glomeruli. RBP4 expressed from an ectopic source efficiently delivers retinol to the eyes, and its deficiency affects night vision largely. Recently, RBP4 as an adipokine, is found to be expressed in adipose tissue and correlated with obesity, insulin resistance (IR) and type 2 diabetes (T2DM). The activity of recombinant human RBP4 was measured by its ability to bind all-trans retinoic acid. The binding of retinoic acid results in the quenching of Trp fluorescence in RBP4. RBP4 was diluted to 50 ug/ml in 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, pH 7.5 (assay buffer) and the retinoic acid was diluted to 100, 30, 10, 3 and 1 uM in 95% ethanol. Mixing 112.5 uL of 50 ug/mL rhRBP4 and 12.5 uL of retinoic acid serial dilutions in microtubes and a blank containing 112.5 uL of 50 ug/mL rhRBP4 and 12.5 uL of 95% ethanol, then incubate at room temperature for 30 minutes. Loading 100 ul of the reaction mixtures and blank and read at excitation and emission wavelengths of 280 nm and 340 nm (top read), respectively, in endpoint mode. The result was shown in figure 1, the 50% binding concentration (BC50) is >30 uM.)
Retinol Binding Protein 4 (RBP4), Active Protein (Cat# AAA161805)
Full Name
Active Retinol Binding Protein 4 (RBP4)
Gene Names
RBP4; RDCCAS
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(Retinol-binding protein 4 (RBP4) is the specific carrier for retinol (also known as vitamin A), and is responsible for the conversion of unstable and insoluble retinol in aqueous solution into stable and soluble complex in plasma through their tight interaction. As a member of the lipocalin superfamily, RBP4 containing a beta-barrel structure with a well-defined cavity is secreted from the liver, and in turn delivers retinol from the liver stores to the peripheral tissues. In plasma, the RBP4-retinol complex interacts with transthyretin (TTR), and this binding is crucial for preventing RBP4 excretion through the kidney glomeruli. RBP4 expressed from an ectopic source efficiently delivers retinol to the eyes, and its deficiency affects night vision largely. Recently, RBP4 as an adipokine, is found to be expressed in adipose tissue and correlated with obesity, insulin resistance (IR) and type 2 diabetes (T2DM). The activity of recombinant rat RBP4 was measured by its ability to bind all-trans retinoic acid. The binding of retinoic acid results in the quenching of Trp fluorescence in RBP4. RBP4 was diluted to 50 ug/ml in 50 mM Tris, 10 mM CaCl2, 150 mM NaCl, pH 7.5 (assay buffer) and the retinoic acid was diluted to 800, 400, 200, 100, 30, 10, 3, 1, 0.5 and 0.1 uM in 95% ethanol. Mixing 112.5 uL of 50 ug/mL rrRBP4 and 12.5 uL of retinoic acid serial dilutions in microtubes and a blank containing 112.5 uL of 50 ug/mL rrRBP4 and 12.5 uL of 95% ethanol, then incubate at room temperature for 30 minutes. Loading 100 ul of the reaction mixtures and blank and read at excitation and emission wavelengths of 280 nm and 340 nm (top read), respectively, in endpoint mode. The result was shown in figure 1, the 50% binding concentration (BC50) is >22 uM.)
Retinol Binding Protein 4 (RBP4), Active Protein (Cat# AAA161808)
Full Name
Active Retinol Binding Protein 4 (RBP4)
Gene Names
Rbp4; RBPA
Applications
Activity Assay, Cell Culture
Purity
Greater than 95% by SDS-PAGE
Pricing
Bioactivity
(Glypican-3 (GPC3), a 70 kDa protein, is a member of the glypican family that attaches to the cell surface by a glycosylphosphatidylinositol anchor, is specifically up-regulated in hepatocellular carcinoma (HCC) although rarely or not expressed in normal liver tissues, making it a perfect test and treatment target for HCC. GPC3 is also a negative transcriptional regulator and tumor suppressor that inhibits the growth of breast, ovary, and lung cancer cells. It is reported that GPC3 can form a complex with insulin-like growth factor 2 (IGF2), and might thereby modulate IGF2 action. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant mouse GPC3 and recombinant rat IGF2. Briefly, GPC3 was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to IGF2-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-GPC3 pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant mouse GPC3 and recombinant rat IGF2 was shown in Figure 1, the EC50 for this effect is 0.12 ug/mL.)
Glypican 3 (GPC3), Active Protein (Cat# AAA161815)
Full Name
Active Glypican 3 (GPC3)
Gene Names
Gpc3; OCI-5
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(N-Myristoyltransferase 1 (NMT1) is an indispensable eukaryotic enzyme that belongs to the NMT family. NMT1 can catalyses the transfer of myristoyl groups to the amino acid terminal residues of numerous proteins. This catalytic process is required for the growth and development of many eukaryotes and viruses. Furthermore, it is recognized that N-myristoylation can also occur posttranslationally on an internal glycine exposed by caspase cleavage during apoptosis. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant human NMT1 and recombinant human CASP3. Briefly, biotin-linked NMT1 were diluted serially in PBS, with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to CASP3-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST 3 times and incubation with Streptavidin-HRP for 30min, then wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 ul stop solution to the wells and read at 450 nm immediately. The binding activity of recombinant human NMT1 and recombinant human CASP3 was shown in Figure 1, the EC50 for this effect is 0.01 ug/mL.)
N-Myristoyltransferase 1 (NMT1), Active Protein (Cat# AAA161922)
Full Name
Active N-Myristoyltransferase 1 (NMT1)
Gene Names
NMT1; NMT
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
Bioactivity
(IFN-g is a dimerized soluble cytokine that is the only member of the type II class of interferons. The importance of IFNg in the immune system stems in part from its ability to inhibit viral replication directly and most importantly from its immunostimulatory and immunomodulatory effects. Studies show that IFN-gamma can rapidly regulate STAT activation by IL-10 and alters macrophage responses to IL-10. Thus a functional binding ELISA assay was conducted to detect the interaction of recombinant pig IFN-g and recombinant pig IL-10. Briefly, IFN-g was diluted serially in PBS with 0.01% BSA (pH 7.4). Duplicate samples of 100 ul were then transferred to IL-10-coated microtiter wells and incubated for 1h at 37 degree C. Wells were washed with PBST and incubated for 1h with anti-IFN-g pAb, then aspirated and washed 3 times. After incubation with HRP labelled secondary antibody for 1h at 37 degree C, wells were aspirated and washed 5 times. With the addition of substrate solution, wells were incubated 15-25 minutes at 37 degree C. Finally, add 50 uL stop solution to the wells and read at 450/630 nm immediately. The binding activity of recombinant pig IFN-g and recombinant pig IL-10 was shown in Figure 1, the EC50 for this effect is 3.1 ug/mL.)
Interferon Gamma (IFNg), Active Protein (Cat# AAA161669)
Full Name
Active Interferon Gamma (IFNg)
Applications
Activity Assay, Cell Culture
Purity
Greater than 90% by SDS-PAGE
Pricing
SDS-PAGE
DAPK1 / DAP Kinase 1, Active Protein (Cat# AAA173507)
Full Name
Recombinant Human DAPK1 / DAP Kinase 1 Protein (aa 1-363, His & GST tag)
Gene Names
DAPK1; DAPK
Purity
(78.5 + 12.2%) as determined by SDS-PAGE
Pricing
SDS-PAGE
IL13RA2 / CD213A2, Active Protein (Cat# AAA173596)
Full Name
Recombinant Mouse IL13RA2 / CD213A2 Protein (His tag)
Gene Names
Il13ra2; CD213a2; IL-13R-alpha-2
Purity
> 98% as determined by SDS-PAGE
Pricing
Application Data
VEGF165, Active Protein (Cat# AAA79271)
Full Name
Human VEGF165
Gene Names
VEGFA; VPF; VEGF; MVCD1
Reactivity
Human
Purity
> 95% by SDS-PAGE & silver stain
Pricing
CTGF, Active Protein (Cat# AAA75454)
Full Name
CTGF protein
Gene Names
CTGF; CCN2
Purity
> 95% pure
Pricing
FasL, Active Protein (Cat# AAA75199)
Full Name
FasL protein
Gene Names
FASLG; APTL; FASL; CD178; CD95L; ALPS1B; CD95-L; TNFSF6; APT1LG1
Purity
> 98% by SDS-PAGE gel and HPLC Analyses.
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized human PD-L2 at 2 ug/ml (100 ul/well) can bind human PD-1, the EC50 of human PD-1 is 150-800 ng/mL.)
PD-L2, Active Protein (Cat# AAA257866)
Full Name
PD-L2 Protein, Human, Recombinant (ECD, His Tag)
Gene Names
PDCD1LG2; B7DC; Btdc; PDL2; CD273; PD-L2; PDCD1L2; bA574F11.2
Purity
>98% as determined by SDS-PAGE
Pricing
Application Data
(Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts.The ED50 for this effect is typically 0.4-2ng/mL.)
HB-EGF, Active Protein (Cat# AAA257870)
Full Name
HB-EGF Protein, Human, Recombinant
Gene Names
HBEGF; DTR; DTS; DTSF; HEGFL
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized recombinant Human HVEM-His at 10 ug/ml (100 ul/well) can bind human BTLA-Fc with a linear range of 1.28-20 ug/ml.)
HVEM, Active Protein (Cat# AAA257872)
Full Name
HVEM Protein, Human, Recombinant (ECD, His Tag)
Gene Names
TNFRSF14; TR2; ATAR; HVEA; HVEM; CD270; LIGHTR
Purity
>90% as determined by SDS-PAGE
Pricing
Application Data
(Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 5-25 ng/mL.)
LIGHT, Active Protein (Cat# AAA257879)
Full Name
LIGHT Protein, Human, Recombinant (rFc Tag)
Purity
>90% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized human BID at 10 ug/mL (100 ul/well) can bind biotinylated mouse BCL2L1, The EC50 of biotinylated mouse BCL2L1 is 5.6 ng/mL.)
BID, Active Protein (Cat# AAA257888)
Full Name
BID Protein, Human, Recombinant
Gene Names
BID; FP497
Purity
>90% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its ability to inhibit FGF acidic (aFGF / FGF1) dependent proliferation of Balb/c3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 5-25ng/mL.)
FGFR4, Active Protein (Cat# AAA257891)
Full Name
FGFR4 Protein, Human, Recombinant (hFc Tag)
Gene Names
FGFR4; TKF; JTK2; CD334
Purity
>97% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its ability to inhibit FGF acidic (aFGF / FGF1) dependent proliferation of Balb/c3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.2-1ug/mL.)
FGFR4, Active Protein (Cat# AAA257892)
Full Name
FGFR4 Protein, Human, Recombinant (His Tag)
Gene Names
FGFR4; TKF; JTK2; CD334
Purity
>90% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized VEGF C-his at 2 ug/mL (100 uL/well) can bind VEGFR3-His, Biotinylated, the EC50 of VEGFR3-His, Biotinylated is 1-10 ng/mL.)
VEGF C, Active Protein (Cat# AAA257893)
Full Name
VEGF C Protein, Human, Recombinant (His Tag)
Gene Names
VEGFC; VRP; Flt4-L
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized CD155a-His at 10 ug/mL (100 uL/well) can bind CD226-Fc-10565-H02H, the EC50 of human CD226-Fc is 15-40 ng/mL.)
CD226, Active Protein (Cat# AAA257895)
Full Name
CD226 Protein, Human, Recombinant (ECD, hFc Tag)
Gene Names
CD226; PTA1; DNAM1; DNAM-1; TLiSA1
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized human CD226 at 2 ug/ml (100 ul/well) can bind human CD112 with a linear ranger of 6.4-800 ng/ml.)
CD226, Active Protein (Cat# AAA257896)
Full Name
CD226 Protein, Human, Recombinant (ECD, His Tag)
Gene Names
CD226; PTA1; DNAM1; DNAM-1; TLiSA1
Purity
>97% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized Human PDGF-B His at 2 ug/ml (100 ul/well) can bind Human PDGFRBHis & hFc, the EC50 of Human PDGFRBHis & hFc is 9.0-40.0 ng/mL.)
PDGF-B, Active Protein (Cat# AAA257897)
Full Name
PDGF-B Protein, Human, Recombinant (His Tag)
Gene Names
PDGFB; SIS; SSV; PDGF2; c-sis; PDGF-2
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its ability to inhibit FGF acidic dependent proliferation of Balb/C 3T3 mouse fibroblasts. The ED50 for this effect is typically 0.1-0.6 ug/mL.)
FGFR1, Active Protein (Cat# AAA257902)
Full Name
FGFR1 Protein, Human, Recombinant (His Tag)
Gene Names
FGFR1; CEK; FLG; HH2; OGD; ECCL; FLT2; KAL2; BFGFR; CD331; FGFBR; FLT-2; HBGFR; N-SAM; FGFR-1; HRTFDS; bFGF-R-1
Purity
>98% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized Mouse TNFSF13 at 2 ug/mL (100 ul/well) can bind Human BCMA, The EC50 of Human BCMA is 0.6-2.1 ng/mL.)
BCMA, Active Protein (Cat# AAA257903)
Full Name
BCMA Protein, Human, Recombinant (ECD, His Tag)
Gene Names
TNFRSF17; BCM; BCMA; CD269; TNFRSF13A
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Immobilized human GST-SerpinB2 at 10 ug/ml (100 ul/well) can bind biotinylated human uPA-His, The EC50 of biotinylated human uPA-His is 10.24-23.88 ng/ml.)
SerpinB2, Active Protein (Cat# AAA257905)
Full Name
SerpinB2 Protein, Human, Recombinant (GST Tag)
Gene Names
SERPINB2; PAI; PAI2; PAI-2; PLANH2; HsT1201
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
SFRP1, Active Protein (Cat# AAA257909)
Full Name
SFRP1 Protein, Human, Recombinant (His Tag)
Gene Names
SFRP1; FRP; FRP1; FrzA; FRP-1; SARP2
Purity
>97% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized human VEGFC-His at 10 ug/mL (100 uL/well) can bind human NRP2-Fc, the EC50 of human NRP2-Fc is 0.1-0.5 ug/mL.)
Neuropilin-2, Active Protein (Cat# AAA257915)
Full Name
Neuropilin-2 Protein, Human, Recombinant (hFc Tag)
Gene Names
NRP2; NP2; NPN2; PRO2714; VEGF165R2
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its binding ability in a functional ELISA. Immobilized B7-1 Protein, Human, Recombinant (His Tag) at 2 ug/ml (100 ul/well) can bind CTLA-4 Protein, Human, Recombinant (His & hFc Tag). The EC50 of of CTLA-4 (His & hFc Tag) is 35-80 ng/ml.)
B7-1, Active Protein (Cat# AAA257916)
Full Name
B7-1 Protein, Human, Recombinant (ECD, His Tag)
Gene Names
CD80; B7; BB1; B7-1; B7.1; LAB7; CD28LG; CD28LG1
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its ability to inhibit EPO-dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 15-60 ng/mL in the presence of 0.1 U/mL Recombinant Human EPO.)
EPOR, Active Protein (Cat# AAA257919)
Full Name
EPOR Protein, Human, Recombinant (ECD, His Tag)
Gene Names
EPOR; EPO-R
Purity
>98% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T 1/2 2A6 mouse embryonal fibroblast cells. The ED50 for this effect is typically 1-6 ug/mL in the presence of 20 ng/mL of Wnt3a.)
SFRP4, Active Protein (Cat# AAA257921)
Full Name
SFRP4 Protein, Human, Recombinant (His Tag)
Gene Names
SFRP4; FRP-4; FRPHE; sFRP-4
Purity
>90% as determined by SDS-PAGE
Pricing
Application Data
(Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T 1/2 2A6 mouse embryonal fibroblast cells. The ED50 for this effect is typically 2-20 ug/mL in the presence of 20 ng/mL of Wnt3a.)
SFRP4, Active Protein (Cat# AAA257922)
Full Name
SFRP4 Protein, Human, Recombinant (His Tag)
Gene Names
SFRP4; FRP-4; FRPHE; sFRP-4
Purity
>95% as determined by SDS-PAGE
Pricing
Application Data
(Immobilized human His-NKG2D (78-216) at 10 ug/ml (100 ul/well) can bind human MICB-Fch, The EC50 of human MICB-Fch is 15.9-37.1 ng/ml.)
MICB, Active Protein (Cat# AAA257924)
Full Name
MICB Protein, Human, Recombinant (His & hFc Tag)
Gene Names
MICB; PERB11.2
Purity
>98% as determined by SDS-PAGE
Pricing
What Are Active Proteins?
Proteins are large molecules made up of long chains of amino acids.
They will typically fold into a very particular 3-dimensional shape/conformation, that is sometimes referred to as their “native” form, which allows them to work properly in the body. For the purposes of product categorization, AAA Biotech will typically refer to proteins purified from their original animal host as being “native” proteins (this is to signify their difference compared to their “recombinant” or “synthetic” protein counterparts).
If a protein successfully folds into the correct shape, it is will typically display high fidelity characteristics to its original protein in its original animal host, and be classified as an active protein, as it will be able to function “normally” in most enzymatic or binding capacities. If it loses this shape, due to factors such as heat or strong chemicals (such as detergents), it becomes inactive and is no longer able to perform its basic functions. All of the proteins in this category are made under strict quality control, and they are active, pure, low in contaminants, and stable.
Most are stored as freeze-dried powders and come without extra tags, so they’re very close to the actual natural/native form.
Key Applications of Active Proteins
1. Scientific Research
- Aid in the study of how proteins function in the body
- Aid in understanding various disease processes
2. Drug Development
- Powerful tools to investigate how potential drugs interact with specific proteins
- Ideal for identifying drug targets
3. Cell Culture
- Are routinely utilized to support cell growth and function (e.g., using exogenous growth factors)
- Can be used to promote cellular development into specific types (differentiation)
4. Diagnostics
- Regularly utilized in tests to detect diseases or infections (e.g., COVID-19, cancer)
- Note: All products are strictly for research-use only (RUO).
5. Therapeutics
- Some active proteins are used directly as treatments (e.g., insulin, enzymes)
- Note: All products are strictly for research-use only (RUO).
6. Vaccine Development
- Used to create or test vaccines by mimicking parts of viruses or bacteria
7. Biochemical Assays
- They can facilitate the characterization of enzyme activity, binding strength, or protein interactions in lab tests
Why Buy Active Proteins from AAA Biotech?
- High biological activity – Verified to perform as expected or indicated on datasheet
- Strict quality control – We are confident in our active proteins’ reliability and consistency
- High purity & low endotoxin – Ideal for applications involving sensitive or precious samples/components
- Freeze-dried for stability – Long shelf life and straightforward storage
- Mostly tag-free – Closer to natural/native protein form
FAQ
1. What are active proteins used for in research?
Active proteins are used primarily in the study of how proteins function, in characterizing/discovering drug interactions, supporting cell growth, running biochemical assays, and in development of diagnostics or therapeutics.
2. How are AAA Biotech's active proteins validated?
AAA Biotech’s active proteins are validated through strict quality control and functional assays to ensure they are properly folded and active. “Active”, though, can be an ambiguous term, so if a specific “activity” or “binding” capability of a protein is of crucial interest to you, please inquire with us prior to purchase, and we will provide further details on how the “Active” modifier was determined to be applicable.
3. Are these proteins tested for biological activity?
Yes, all active proteins from AAA Biotech are tested to confirm they have the expected biological activity before being offered for use. Though, said “biological activity” can be either “enzymatic”, “binding”, or both.