Bioactivity
(Human 20kDa Growth Hormone stimulates cell proliferation of the Nb2-11 Rat lymphoma cells. The ED50 range ?1 ng/ml.)
20kDa Growth Hormone Active Protein | GH1 active protein
20kDa Growth Hormone, 27-202 aa, Human, E Coli (Bioactivity Validated)
Gene Names
GH1; GH; GHN; GH-N; GHB5; hGH-N; IGHD1B
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
20kDa Growth Hormone; N/A; 20kDa Growth Hormone, 27-202 aa, Human, E Coli (Bioactivity Validated); hGH-V; GH2; Growth hormone 2 isoform 4; HCG1749481; isoform CRA_t; Placental growth hormone 20kDa isoform; Pituitary growth hormone; Growth hormone 1; GH; GH 1; GH N; GH-N; GH1; GHN; Growth hormone; Growth hormone 1 Pituitary growth hormone; Growth hormone 1 variant 1; Growth hormone 1 variant 2; Growth Hormone Normal; Growth Hormone Pituitary; normal; pituitary; HG1; hGH N; hGH-N; HGHN; IGHD1B; Pituitary Growth Hormone; RNGHGP; Somatotropin; Somatotropin C; Somatotropin I Precursor,; GH1 active protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In phosphate buffer saline (pH 7.4) containing 10% glycerol
Concentration
1 mg/ml (determined by BCA assay) (varies by lot)
Sequence
MFPTIPLSRL FDNAMLRAHR LHQLAFDTYQ EFNPQTSLCF SESIPTPSNR EETQQKSNLE LLRISLLLIQ SWLEPVQFLR SVFANSLVYG ASDSNVYDLL KDLEEGIQTL MGRLEDGSPR TGQIFKQTYS KFDTNSHNDD ALLKNYGLLY CFRKDMDKVE TFLRIVQCRS VEGSCGF
Sequence Length
217
Applicable Applications for GH1 active protein
Bioactivity, SDS-PAGE
Tag
Not tagged
Endotoxin Level
< 1.0 EU per 1 microgram of protein (determined by LAL method)
Biological Activity
Measured in a cell proliferation assay using Nb2-11 Rat lymphoma cells. The ED50 for this effect is less or equal to 1ng/ml.
Preparation and Storage
Can be stored at 2-8 degree C short term for one week
For long term storage, aliquot and store at -20 degree C or -80 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -80 degree C.
Avoid repeated freezing and thawing cycles.
Bioactivity
(Human 20kDa Growth Hormone stimulates cell proliferation of the Nb2-11 Rat lymphoma cells. The ED50 range ?1 ng/ml.)
Bioactivity
(Human 20kDa Growth Hormone stimulates cell proliferation of the Nb2-11 Rat lymphoma cells. The ED50 range ?1 ng/ml.)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
SDS-PAGE
(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain.)
Related Product Information for GH1 active protein
The 20KDa variant form of human growth hormone (20KDa hGH) presents in extracts from pituitary glands and it differs from the major form of hGH (22K, 191 amino acid) by the deletion of amino acid residues 32-46. The physiological role of 20K hGH remains to be determined partly but it regards the main function as stimulation of somatic and bone growth, as well as an increase in the size and mass of organs and tissues. Recombinant 20KDa human growth hormone was purified by FPLC gel-filtration chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.
Product Categories/Family for GH1 active protein
References
Frigeri, et al (1979) Biochem Biophys Res Commun. 14; 778-82; Adelman, et al (1983) DNA. 2; 183-93; Tsushima, et al (2000) Endocr J. 47; S17-21; Horan, M.,et al (2003) Hum. Mutat. 21 (4), 408-423;
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
20 kDa (177 aa), confirmed by MALDI-TOF.
NCBI Official Full Name
somatotropin isoform 1
NCBI Official Synonym Full Names
growth hormone 1
NCBI Official Symbol
GH1
NCBI Official Synonym Symbols
GH; GHN; GH-N; GHB5; hGH-N; IGHD1B
NCBI Protein Information
somatotropin
UniProt Protein Name
Somatotropin
UniProt Gene Name
GH1
UniProt Synonym Gene Names
GH; GH-N
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Product Notes
The GH1 gh1 (Catalog #AAA48455) is an Active Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. AAA Biotech's 20kDa Growth Hormone can be used in a range of immunoassay formats including, but not limited to, Bioactivity, SDS-PAGE. Researchers should empirically determine the suitability of the GH1 gh1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MFPTIPLSRL FDNAMLRAHR LHQLAFDTYQ EFNPQTSLCF SESIPTPSNR EETQQKSNLE LLRISLLLIQ SWLEPVQFLR SVFANSLVYG ASDSNVYDLL KDLEEGIQTL MGRLEDGSPR TGQIFKQTYS KFDTNSHNDD ALLKNYGLLY CFRKDMDKVE TFLRIVQCRS VEGSCGF. It is sometimes possible for the material contained within the vial of "20kDa Growth Hormone, Active Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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