Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded rat heart using SNRPA Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using SNRPA Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat liver using SNRPA Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded rat heart using SNRPA Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using SNRPA Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using SNRPA Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast using SNRPA Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human adenomyosis using SNRPA Antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using SNRPA antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

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SNRPA, Polyclonal Antibody (Cat# AAA28084)

Full Name

SNRPA Polyclonal Antibody

Gene Names

SNRPA; U1A; Mud1; U1-A

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse heart using BAZ1B antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse stomach using BAZ1B antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using BAZ1B antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse lung using BAZ1B antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using BAZ1B antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using BAZ1B antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)

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BAZ1B, Polyclonal Antibody (Cat# AAA28215)

Full Name

BAZ1B Polyclonal Antibody

Gene Names

BAZ1B; WSTF; WBSCR9; WBSCR10

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Affinity purification

Pricing

$285/0.02 mL $500/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,780/4x0.1 mL $1,960/5x0.1 mL

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FCM (Flow Cytometry) (SEPT9 Antibody (A555) flow cytometric analysis of HepG2 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohistochemistry) (SEPT9 Antibody (A555) immunohistochemistry analysis in formalin fixed and paraffin embedded kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of SEPT9 Antibody (A555) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot) (The anti-SEPT9 Pab is used in Western blot to detect SEPT9 in Jurkat cell lysate.)

IHC (Immunohistochemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot) (Western blot analysis of lysates from human kidney and liver tissue lysate (from left to right), using SEPT9 Antibody (C-term). AAA28793 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

WB (Western Blot) (Western blot analysis of lysates from A431, Hela cell line (from left to right), using SEPT9 Antibody (C-term). AAA28793 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

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SEPT9, Polyclonal Antibody (Cat# AAA28793)

Full Name

SEPT9 Antibody (C-term)

Gene Names

SEPT9; MSF; MSF1; NAPB; SINT1; PNUTL4; SeptD1; AF17q25

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$275/0.08 mL $510/0.4 mL $975/2x0.4 mL $1,415/3x0.4 mL $1,825/4x0.4 mL $2,070/5x0.4 mL

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

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HDAC5/9, Polyclonal Antibody (Cat# AAA29035)

Full Name

HDAC5/9 Polyclonal Antibody

Gene Names

HDAC5; HD5; NY-CO-9

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

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Rb, Polyclonal Antibody (Cat# AAA29107)

Full Name

Rb Polyclonal Antibody

Gene Names

RB1; RB; pRb; OSRC; pp110; p105-Rb

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/40000. Not yet tested in other applications.)

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TdT, Polyclonal Antibody (Cat# AAA29129)

Full Name

TdT Polyclonal Antibody

Gene Names

DNTT; TDT

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

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IHC (Immunohistochemistry) (TECTA was detected in paraffin-embedded sections of human testis tissues using rabbit anti- TECTA Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)

IHC (Immunohistochemistry) (TECTA was detected in paraffin-embedded sections of human lung cancer tissues using rabbit anti- TECTA Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)

IHC (Immunohistochemistry) (TECTA was detected in paraffin-embedded sections of human intetsinal cancer tissues using rabbit anti- TECTA Antigen Affinity purified polyclonal antibody at 1ug/mL. The immunohistochemical section was developed using SABC method.)

WB (Western Blot) (Western blot analysis of TECTA expression in rat testis extract (lane 1), HEPA1-6 whole cell lysates (lane 2) and HEPG2 whole cell lysates (lane 3). TECTA at 239KD was detected using rabbit anti- TECTA Antigen Affinity purified polyclonal antibody at 0.5ug/mL. The blot was developed using chemiluminescence (ECL) method.)

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TECTA, Polyclonal Antibody (Cat# AAA11686)

Full Name

Anti-TECTA Antibody

Gene Names

TECTA; DFNA8; DFNA12; DFNB21

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of HEL cells using anti-GNAI2 antibody (AAA19777).Overlay histogram showing HEL cells stained with AAA19777 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GNAI2 Antibody (AAA19777, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 8. IF analysis of GNAI2 using anti-GNAI2 antibody (AAA19777).GNAI2 was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GNAI2 Antibody (AAA19777) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GNAI2 Antibody (AAA19777) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GNAI2 Antibody (AAA19777) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GNAI2 Antibody (AAA19777) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GNAI2 Antibody (AAA19777) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GNAI2 Antibody (AAA19777) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HEL whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GNAI2 antigen affinity purified polyclonal antibody (#AAA19777) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GNAI2 at approximately 40 kDa. The expected band size for GNAI2 is at 40 kDa.)

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GNAI2, Polyclonal Antibody (Cat# AAA19777)

Full Name

Anti-GNAI2 Antibody Picoband

Gene Names

GNAI2; GIP; GNAI2B; H_LUCA15.1; H_LUCA16.1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of Hela cells using anti-MOSPD2 antibody (AAA20007).Overlay histogram showing Hela cells stained with AAA20007 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MOSPD2 Antibody (AAA20007, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of Caco-2 cells using anti-MOSPD2 antibody (AAA20007).Overlay histogram showing Caco-2 cells stained with AAA20007 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MOSPD2 Antibody (AAA20007, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of MOSPD2 using anti-MOSPD2 antibody (AAA20007).MOSPD2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MOSPD2 Antibody (AAA20007) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MOSPD2 Antibody (AAA20007) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MOSPD2 Antibody (AAA20007) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MOSPD2 Antibody (AAA20007) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MOSPD2 Antibody (AAA20007) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MOSPD2 Antibody (AAA20007) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human U251 whole cell lysates,Lane 3: rat C6 whole cell lysates,Lane 4: rat RH35 whole cell lysates,Lane 5: rat PC-12 whole cell lysates,Lane 6: mouse Neuro-2a whole cell lysates,Lane 7: mouse HEPA1-6 whole cell lysates,Lane 8: mouse RAW264.7 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MOSPD2 antigen affinity purified polyclonal antibody (#AAA20007) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MOSPD2 at approximately 60 kDa. The expected band size for MOSPD2 is at 60 kDa.)

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MOSPD2, Polyclonal Antibody (Cat# AAA20007)

Full Name

Anti-MOSPD2 Antibody Picoband

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 200ug extracts of 293T cells usingug CDK4 Polyclonal Antibody. Western blot was performed from the immunoprecipitate using CDK4 Polyclonal Antibody at a dilution of 1:1000.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 using CDK4 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon using CDK4 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of extracts from wild type(WT) and CDK4 knockout (KO) HeLa cells using CDK4 Polyclonal Antibody at 1:1000 dilution.)

WB (Western Blot) (Western blot analysis of extracts of 293T cells using CDK4 Polyclonal Antibody at 1:1000 dilution.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines using CDK4 Polyclonal Antibody at 1:1000 dilution.)

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CDK4, Polyclonal Antibody (Cat# AAA22315)

Full Name

(KO Validated) CDK4 Polyclonal Antibody

Gene Names

CDK4; CMM3; PSK-J3

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunoprecipitation, Immunofluorescence

Purity

Affinity purification

Pricing

$340/0.06 mL $475/0.12 mL $710/0.2 mL $1,365/2x0.2 mL $1,975/3x0.2 mL $2,550/4x0.2 mL $3,100/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts of HCT116 cells, using Bcl-2 antibody at 1:1000 dilution.HCT116 cells treated with different concentrations of B7-H4.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using Bcl-2 antibody at 1:1000 dilution.)

IF (Immunofluorescence) (Immunofluorescence analysis of L929 cells using Bcl-2 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse pancreas using Bcl-2 antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using Bcl-2 antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat pancreas using Bcl-2 antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human stomach using Bcl-2 antibody at dilution of 1:100 .)

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Bcl-2, Polyclonal Antibody (Cat# AAA30544)

Full Name

Bcl-2 Polyclonal Antibody

Gene Names

BCL2; Bcl-2; PPP1R50

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

$360/0.05 mL $450/0.1 mL $855/2x0.1 mL $1,230/3x0.1 mL $1,595/4x0.1 mL $1,750/5x0.1 mL

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IHC (Immunohistchemistry) (At 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis NDEL1 (Phospho-Thr219) using UV treated Jurkat whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

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NDEL1, Polyclonal Antibody (Cat# AAA31462)

Full Name

Phospho-NDEL1 (Thr219) Antibody

Gene Names

NDEL1; EOPA; NDE2; NUDEL; MITAP1; NDE1L1

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (100%), Xenopus (100%)

Applications

Western Blot, Immunohistochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$345/0.05 mL $415/0.1 mL $485/0.2 mL $925/2x0.2 mL $1,340/3x0.2 mL $1,725/4x0.2 mL $1,900/5x0.2 mL

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IF (Immunofluorescence) (Figure 12. IF analysis of PLBD2 using anti-PLBD2 antibody (AAA19979).PLBD2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5ug/mL rabbit anti-PLBD2 Antibody (AAA19979) overnight at 4 degree C. DyLight594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

FCM (Flow Cytometry) (Figure 11. Flow Cytometry analysis of HepG2 cells using anti-PLBD2 antibody (AAA19979).Overlay histogram showing HepG2 cells stained with AAA19979 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PLBD2 Antibody (AAA19979, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 10. IF analysis of PLBD2 using anti-PLBD2 antibody (AAA19979).PLBD2 was detected in an immunocytochemical section of U2OS cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PLBD2 Antibody (AAA19979) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PLBD2 Antibody (AAA19979) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PLBD2 Antibody (AAA19979) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PLBD2 Antibody (AAA19979) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PLBD2 Antibody (AAA19979) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PLBD2 Antibody (AAA19979) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PLBD2 Antibody (AAA19979) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-PLBD2 Antibody (AAA19979) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human A549 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PLBD2 antigen affinity purified polyclonal antibody (#AAA19979) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PLBD2 at approximately 75 kDa. The expected band size for PLBD2 is at 66 kDa.)

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PLBD2, Polyclonal Antibody (Cat# AAA19979)

Full Name

Anti-PLBD2 Antibody Picoband

Gene Names

PLBD2; P76

Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using BCAP29 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse spleen using BCAP29 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human stomach using BCAP29 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human prostate using BCAP29 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using BCAP29 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat heart using BCAP29 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using BCAP29 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using BCAP29 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 30s.)

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BCAP29, Polyclonal Antibody (Cat# AAA28093)

Full Name

BCAP29 Polyclonal Antibody

Gene Names

BCAP29; BAP29

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse liver using HIRIP3 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human kidney cancer using HIRIP3 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human kidney using HIRIP3 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human well-differentiated squamous skin carcinoma using HIRIP3 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human oophoroma using HIRIP3 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using HIRIP3 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat spleen using HIRIP3 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat liver using HIRIP3 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using HIRIP3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

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HIRIP3, Polyclonal Antibody (Cat# AAA28166)

Full Name

HIRIP3 Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human stomach using GNE antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human esophagus using GNE antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon using GNE antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using GNE antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using GNE antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using GNE antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)

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GNE, Polyclonal Antibody (Cat# AAA28213)

Full Name

GNE Polyclonal Antibody

Gene Names

GNE; NM; DMRV; IBM2; Uae1; GLCNE

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Affinity purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse heart using BAZ1B antibody.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse stomach using BAZ1B antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using BAZ1B antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse lung using BAZ1B antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using BAZ1B antibody.)

WB (Western Blot) (Western blot analysis of extracts from normal (control) and BAZ1B knockout (KO) HeLa cells, using BAZ1B antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using BAZ1B antibody.)

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BAZ1B, Polyclonal Antibody (Cat# AAA29857)

Full Name

BAZ1B Polyclonal Antibody

Gene Names

BAZ1B; WSTF; WBSCR9; WBSCR10

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

$360/0.05 mL $450/0.1 mL $855/2x0.1 mL $1,230/3x0.1 mL $1,595/4x0.1 mL $1,750/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of Jurkat cells with CD3E antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining CD3E in Lovo cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining CD3E in Hela cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-CD3E antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD3E antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD3E antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of CD3E on different cell lysates using anti-CD3E antibody at 1/1000 dilution.)

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CD3 epsilon, Polyclonal Antibody (Cat# AAA29969)

Full Name

CD3 epsilon Antibody

Gene Names

CD3E; T3E; TCRE; IMD18

Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry

Purity

Peptide affinity purified

Pricing

$390/0.05 mL $510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,015/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

Application Data (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

Application Data (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

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mTOR, Polyclonal Antibody (Cat# AAA29074)

Full Name

mTOR Polyclonal Antibody

Gene Names

MTOR; FRAP; FRAP1; FRAP2; RAFT1; RAPT1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.)

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CD79a, Polyclonal Antibody (Cat# AAA28987)

Full Name

CD79a Polyclonal Antibody

Gene Names

CD79A; IGA; MB-1

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of A431 cells using anti-BAP31/BCAP31 antibody (AAA19799).Overlay histogram showing A431 cells stained with AAA19799 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-BAP31/BCAP31 Antibody (AAA19799, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 8. IF analysis of BAP31/BCAP31 using anti-BAP31/BCAP31 antibody (AAA19799).BAP31/BCAP31 was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BAP31/BCAP31 Antibody (AAA19799) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BAP31/BCAP31 Antibody (AAA19799) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BAP31/BCAP31 Antibody (AAA19799) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BAP31/BCAP31 Antibody (AAA19799) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BAP31/BCAP31 Antibody (AAA19799) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-BAP31/BCAP31 Antibody (AAA19799) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human A431 whole cell lysates,Lane 3: human Hela whole cell lysates,Lane 4: human placenta tissue lysates,Lane 5: rat liver tissue lysates,Lane 6: rat PC-12 whole cell lysates,Lane 7: mouse liver tissue lysates,Lane 8: mouse NIH/3T3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-BAP31/BCAP31 antigen affinity purified polyclonal antibody (#AAA19799) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for BAP31/BCAP31 at approximately 28 kDa. The expected band size for BAP31/BCAP31 is at 28 kDa.)

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BAP31/BCAP31, Polyclonal Antibody (Cat# AAA19799)

Full Name

Anti-BAP31/BCAP31 Antibody Picoband

Gene Names

BCAP31; CDM; BAP31; 6C6-AG; DXS1357E

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of MCF-7 cells using anti-FAM111B antibody (AAA19994).Overlay histogram showing MCF-7 cells stained with AAA19994 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FAM111B Antibody (AAA19994, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 8. IHC analysis of FAM111B using anti-FAM111B antibody (AAA19994).FAM111B was detected in a paraffin-embedded section of human thyroid cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-FAM111B Antibody (AAA19994) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-FAM111B Antibody (AAA19994) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-FAM111B Antibody (AAA19994) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-FAM111B Antibody (AAA19994) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-FAM111B Antibody (AAA19994) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-FAM111B Antibody (AAA19994) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Hacat whole cell lysates,Lane 3: human RT4 whole cell lysates,Lane 4: human U2OS whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAM111B antigen affinity purified polyclonal antibody (#AAA19994) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Lane 2: human Hela whole cell lysates,Lane 3: human MCF-7 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FAM111B antigen affinity purified polyclonal antibody (#AAA19994) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for FAM111B at approximately 85 kDa. The expected band size for FAM111B is at 85 kDa.)

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FAM111B, Polyclonal Antibody (Cat# AAA19994)

Full Name

Anti-FAM111B Antibody Picoband

Gene Names

FAM111B; CANP; POIKTMP

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

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NFkappaB-p65, Polyclonal Antibody (Cat# AAA28852)

Full Name

NFkappaB-p65 (Acetyl Lys314/Lys315) Polyclonal Antibody

Gene Names

RELA; p65; NFKB3

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

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VEGF-C, Polyclonal Antibody (Cat# AAA29208)

Full Name

VEGF-C Polyclonal Antibody

Gene Names

VEGFC; VRP; Flt4-L

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts of U-251MG cells, using NOTCH1 at 1:1000 dilution.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse testis using NOTCH1 at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human placenta using NOTCH1 at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat testis using NOTCH1 at dilution of 1:200 (40x lens).)

IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using NOTCH1 Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using NOTCH1 Polyclonal at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

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NOTCH1, Polyclonal Antibody (Cat# AAA30646)

Full Name

NOTCH1 Rabbit Polyclonal Antibody

Gene Names

NOTCH1; hN1; TAN1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

$360/0.05 mL $450/0.1 mL $855/2x0.1 mL $1,230/3x0.1 mL $1,595/4x0.1 mL $1,750/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse cancer using LHX4 antibody.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse liver using LHX4 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using LHX4 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human kidney cancer using LHX4 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human oophoroma using LHX4 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using LHX4 antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using LHX4 Antibody.)

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LHX4, Polyclonal Antibody (Cat# AAA29853)

Full Name

LHX4 Polyclonal Antibody

Gene Names

LHX4; CPHD4

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Affinity purification

Pricing

$360/0.05 mL $450/0.1 mL $855/2x0.1 mL $1,230/3x0.1 mL $1,595/4x0.1 mL $1,750/5x0.1 mL

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IHC (Immunohistochemistry) (AAA31076 at 1/200 staining human liver cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistchemistry) (AAA31076 at 1/200 staining human liver cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31076 at 1/200 staining human liver cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (BMP3 Antibody for IHC in human testis.)

WB (Western Blot) (Western blot analysis of extracts of various sample, using BMP3 antibody.)

WB (Western Blot) (Western blot analysis of BMP3 expression in HeLa cells, The lane on the left is treated with the antigen-specific peptide.)

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BMP3, Polyclonal Antibody (Cat# AAA31076)

Full Name

BMP3 Antibody

Gene Names

BMP3; BMP-3A

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

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WB (Western Blot) (Western Blot Analysis: Representative lot data. Lysate from HEK-293 cells was resolved by electrophoresis, transferred to PVDF and probed with anti-PI3 Kinase, p110beta (0.05 ug/mL). Proteins were visualized using donkey anti-rabbit secondary antibody conjugated to HRP and chemiluminescence detection. Arrow indicates PI3 Kinase, p110beta (~110kD).)

IP (Immunoprecipitation) (Immunoprecipitation: 10ug of was used to immunoprecipitate from 500ug of Jurkat whole cell lysate. The antibody was collected on Protein A beads and eluted with sample buffer. 5uL of Jurkat whole cell lysate was then resolved via SDS-PAGE, transferred to PVDF and probed with 0.5ug . Proteins were visualized using anti-rabbit-HRP conjugate and an ECL system.)

ICC (Immunocytochemistry) (Immunocytochemistry: PI3-Kinase, p110b staining of human skeletal muscle. Tissue pre-treated with Citrate pH 6.0 antigen-retrieval. diluted to 1:100, IHC-Select Detection with HRP-DAB. Immunoreactivity is seeing as a staining pattern of cross banding striated muscle fibers.)

ICC (Immunocytochemistry) (Immunocytochemistry: PI3-Kinase, p110b staining in colorectal carcinoma. Tissue pre-treated with Citrate pH 6.0 antigen-retrieval. diluted to 1:100, IHC-Select Detection with HRP-DAB. Immunoreactivity is seeing as a plasma membrane staining pattern.)

ICC (Immunocytochemistry) (Confocal Immunocytochemistry Analysis: HeLa cells were fixed, permeablized, and stained with (Cy3, red), DAPI (blue, nuclei), and Phalloidin-AlexaFluor488 (actin, green). Figure on the left has the DAPI filter off.)

ICC (Immunocytochemistry) (Immunocytochemistry: PI3-Kinase, p110b staining of human kidney 2 mm array spot. Tissue pre-treated with Citrate pH 6.0 antigen-retrieval. diluted to 1:100, IHC-Select Detection with HRP-DAB. Immunoreactivity is see as a staining to include the thin and distal microtubules.)

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Phosphoinositide 3 Kinase, p110 beta, Polyclonal Antibody (Cat# AAA26903)

Full Name

Phosphoinositide 3 Kinase, p110 beta (DKFZp779K1237, MGC133043, p110beta, Phosphatidylinositol 3 Kinase Catalytic beta Polypeptide, Phosphatidylinositol-4,5-bisphosphate 3-kinase Catalytic Subunit beta Isoform, Phosphoinositide 3 Kinase Catalytic beta Pol

Gene Names

PIK3CA; MCM; CWS5; MCAP; PI3K; CLOVE; MCMTC; p110-alpha

Reactivity

Human

Applications

Immunocytochemistry, Western Blot, Immunoprecipitation, Immunohistochemistry

Purity

Purified by Immunoaffinity chromatography.

Pricing

$1,120/0.1 mL $2,155/2x0.1 mL $3,130/3x0.1 mL $4,055/4x0.1 mL $4,750/5x0.1 mL

IHC (Immunohistochemistry) (The image is immunohistochemistry of paraffin-embedded Human tonsil tissue using 47699(GNA12 Antibody) at dilution 1/20.(Original magnification: 200))

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GNA12, Polyclonal Antibody (Cat# AAA29764)

Full Name

GNA12 Antibody

Gene Names

GNA12; RMP; gep; NNX3

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Purity

Antigen affinity purification

Pricing

$365/0.1 mL $680/2x0.1 mL $980/3x0.1 mL $1,260/4x0.1 mL $1,355/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

Application Data (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

Application Data (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

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Ub, Polyclonal Antibody (Cat# AAA29292)

Full Name

Ub Polyclonal Antibody

Gene Names

UBA52; L40; CEP52; RPL40; HUBCEP52

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using SEPT7 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using SEPT7 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse Intestine using SEPT7 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using SEPT7 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat Intestine using SEPT7 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using SEPT7 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

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SEPT7, Polyclonal Antibody (Cat# AAA10767)

Full Name

SEPT7 Polyclonal Antibody

Gene Names

SEPT7; CDC3; CDC10; SEPT7A; NBLA02942

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of SiHa cells using anti-DCTN1/p150-glued antibody (AAA19253).Overlay histogram showing SiHa cells stained with AAA19253 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-DCTN1/p150-glued Antibody (AAA19253, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 8. IF analysis of DCTN1/p150-glued using anti- DCTN1/p150-glued antibody (AAA19253).DCTN1/p150-glued was detected in immunocytochemical section of CACO-2 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5μg/mL rabbit anti- DCTN1/p150-glued Antibody (AAA19253) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of DCTN1/p150-glued using anti-DCTN1/p150-glued antibody (AAA19253).DCTN1/p150-glued was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-DCTN1/p150-glued Antibody (AAA19253) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of DCTN1/p150-glued using anti-DCTN1/p150-glued antibody (AAA19253).DCTN1/p150-glued was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-DCTN1/p150-glued Antibody (AAA19253) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of DCTN1/p150-glued using anti-DCTN1/p150-glued antibody (AAA19253).DCTN1/p150-glued was detected in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-DCTN1/p150-glued Antibody (AAA19253) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of DCTN1/p150-glued using anti-DCTN1/p150-glued antibody (AAA19253).DCTN1/p150-glued was detected in paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-DCTN1/p150-glued Antibody (AAA19253) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of DCTN1/p150-glued using anti-DCTN1/p150-glued antibody (AAA19253).DCTN1/p150-glued was detected in paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-DCTN1/p150-glued Antibody (AAA19253) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of DCTN1/p150-glued using anti-DCTN1/p150-glued antibody (AAA19253).DCTN1/p150-glued was detected in paraffin-embedded section of human ovarian adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-DCTN1/p150-glued Antibody (AAA19253) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of DCTN1/p150-glued using anti-DCTN1/p150-glued antibody (AAA19253).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human Hela whole cell lysatesLane 2: human Jurkat whole cell lysatesLane 3: human placenta tissue lysatesLane 4: human U-87MG whole cell lysatesLane 5: human Caco-2 whole cell lysatesLane 6: human U20S whole cell lysatesLane 7: human HEK293 whole cell lysatesLane 8: human HepG2 whole cell lysatesLane 9: rat brain tissue lysatesLane 10: rat stomach tissue lysatesLane 11: mouse brain tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-DCTN1/p150-glued antigen affinity purified polyclonal antibody (Catalog # AAA19253) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for DCTN1/p150-glued at approximately 150KD. The expected band size for DCTN1/p150-glued is at 150KD.)

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DCTN1/p150-glued, Polyclonal Antibody (Cat# AAA19253)

Full Name

Anti-DCTN1/p150-glued Antibody

Gene Names

DCTN1; P135; DP-150; DAP-150

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

IHC (Immunohistochemistry) (At 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse ovarian tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from Jurkat paclitaxel 8h, using Phospho-Bcl-2 (Thr56) Antibody. Lane1 was treated with phospho-blocking peptide, Lane2 was treated with non-phospho-blocking peptide.)

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Bcl-2, Polyclonal Antibody (Cat# AAA31360)

Full Name

Phospho-Bcl-2 (Thr56) Antibody

Gene Names

BCL2; Bcl-2; PPP1R50

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (86%), Bovine (83%)

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$345/0.05 mL $415/0.1 mL $485/0.2 mL $925/2x0.2 mL $1,340/3x0.2 mL $1,725/4x0.2 mL $1,900/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence of TET3 in human brain tissue with TET3 antibody at 20 μg/mL.)

IHC (Immunohistochemistry) (Immunohistochemistry of TET3 in human brain tissue with TET3 antibody at 2.5 μg/ml.)

WB (Western Blot) (Western blot analysis of TET3 in SK-N-SH cell lysate with TET3 antibody at 1 μg/ml in (A) the absence and (B) the presence of blocking peptide.)

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TET3, Polyclonal Antibody (Cat# AAA10939)

Full Name

TET3 Antibody

Gene Names

TET3; hCG_40738

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

TET3 Antibody is affinity chromatography purified via peptide column.

Pricing

$570/0.1 mg $1,080/2x0.1 mg $1,560/3x0.1 mg $2,015/4x0.1 mg $2,295/5x0.1 mg

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using Smarcd1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using Smarcd1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using Smarcd1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using Smarcd1 antibody (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using Smarcd1 antibody (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using Smarcd1 antibody (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using Smarcd1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.)

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BAF60a, Polyclonal Antibody (Cat# AAA28224)

Full Name

BAF60a Polyclonal Antibody

Gene Names

Smarcd1; Baf60a; D15Kz1; AA407987

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Affinity purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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IHC (Immunohistochemistry) (The image is immunohistochemistry of paraffin embedded Human breast cancer tissue using HSD17B13 Antibody at dilution 1/140.)

IHC (Immunohistochemistry) (The image is immunohistochemistry of paraffin embedded Human liver cancer tissue using HSD17B13 Antibody at dilution 1/140.)

SDS-PAGE (Gel: 8% SDS-PAGE Lysate: 40 ugLane: Rat liver tissue lysatePrimary antibody: HSD17B13 Antibody at dilution 1/500 Secondary antibody: Goat anti rabbit IgG at 1/5000 dilutionExposure time: 1 minute)

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HSD17B13, Polyclonal Antibody (Cat# AAA29646)

Full Name

HSD17B13 Antibody

Gene Names

HSD17B13; SCDR9; NIIL497; SDR16C3; HMFN0376

Reactivity

Human, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Antigen affinity purification.

Pricing

$365/0.1 mL $680/2x0.1 mL $980/3x0.1 mL $1,260/4x0.1 mL $1,355/5x0.1 mL

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IHC (Immunohistchemistry) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

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CD16, Polyclonal Antibody (Cat# AAA29360)

Full Name

CD16 Polyclonal Antibody

Gene Names

FCGR3A; CD16; FCG3; CD16A; FCGR3; IGFR3; IMD20; FCR-10; FCRIII; FCGRIII; FCRIIIA

Reactivity

Human

Applications

Immunohistochemistry

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

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IHC (Immunohistchemistry) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

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CUL-1, Polyclonal Antibody (Cat# AAA29377)

Full Name

CUL-1 Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

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IHC (Immunohistochemistry) (At 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Rat colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of Phospho-IRS-1 (Tyr896) in lysates of 293T insulin, using Phospho-IRS-1 (Tyr896) Antibody(AF4424).)

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IRS1, Polyclonal Antibody (Cat# AAA31366)

Full Name

Phospho-IRS1 (Tyr896) Antibody

Gene Names

IRS1; HIRS-1

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Zebrafish (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%)

Applications

Western Blot, Immunohistochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$345/0.05 mL $415/0.1 mL $485/0.2 mL $925/2x0.2 mL $1,340/3x0.2 mL $1,725/4x0.2 mL $1,900/5x0.2 mL

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

Application Data (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

Application Data (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

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Raf-1, Polyclonal Antibody (Cat# AAA29105)

Full Name

Raf-1 Polyclonal Antibody

Gene Names

RAF1; NS5; CRAF; Raf-1; c-Raf

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

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WB (Western Blot) (Sample: HL-60 Cell (Human) Lysate at 30 ug Primary: Anti-P2Y9 (bs-12074R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 55 kD Observed band size: 55 kD)

WB (Western Blot) (Sample: HL-60(Human) Cell Lysate at 30 ug K562(Human) Cell Lysate at 30 ug Primary: Anti-P2Y9 (bs-12074R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 57 kD)

WB (Western Blot) (Sample: Cerebrum (Mouse) Lysate at 40 ug Ovary (Mouse) Lysate at 40 ug Primary: Anti-P2Y9 (bs-12074R) at 1/1000 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 57 kD)

WB (Western Blot) (Dilution: Sample: Brain (mouse) Lysate at 40 ug Primary: Anti- P2Y9 (bs-12074R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 48 kD)

WB (Western Blot) (Sample: k562 (human)cell Lysate at 40 ug Primary: Anti- P2Y9 (bs-12074R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 42 kD Observed band size: 48 kD)

IHC (Immunohistochemistry-Paraffin) (Paraformaldehyde-fixed, paraffin embedded (Human brain glioma); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (P2Y9) Polyclonal Antibody, Unconjugated (bs-12074R) at 1:400 overnight at 4 degree C, followed by operating according to SP Kit(Rabbit) (sp-0023) instructionsand DAB staining.)

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P2Y9, Polyclonal Antibody (Cat# AAA28814)

Full Name

P2Y9 Polyclonal Antibody

Gene Names

Lpar4; LPA4; p2y9; Gpr23; 5730485F04Rik

Reactivity

Rat, Pig, Dog, Cow

Applications

Western Blot, Immunohistochemistry, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of mouse pancreas using PNLIPRP1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of rat pancreas using PNLIPRP1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of rat pancreas using PNLIPRP1 antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse pancreas using PNLIPRP1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human appendix using PNLIPRP1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat pancreas using PNLIPRP1 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using PNLIPRP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 5s.)

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PNLIPRP1, Polyclonal Antibody (Cat# AAA28353)

Full Name

PNLIPRP1 Rabbit pAb

Gene Names

PNLIPRP1; PLRP1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using SIRT3 antibody at dilution of 1:200. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using SIRT3 antibody at dilution of 1:200. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using SIRT3 Rabbit pAb at dilution of 1:50 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using SIRT3 Rabbit pAb at dilution of 1:50 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using SIRT3 Rabbit pAb at dilution of 1:50 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts from normal (control) and SIRT3 knockout (KO) 293T cells, using SIRT3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)

WB (Western Blot) (Western blot analysis of extracts of HepG2 cells, using SIRT3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)

WB (Western Blot) (Western blot analysis of extracts of Rat liver, using SIRT3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 180s.)

WB (Western Blot) (Western blot analysis of extracts of Mouse liver, using SIRT3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)

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SIRT3, Polyclonal Antibody (Cat# AAA28346)

Full Name

SIRT3 Rabbit pAb

Gene Names

SIRT3; SIR2L3

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

$285/0.02 mL $500/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,780/4x0.1 mL $1,960/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts of various cell lines, using GABARAP at 1:1000 dilution.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse heart using GABARAP at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using GABARAP at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver using GABARAP at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat heart using GABARAP at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using GABARAP at dilution of 1:100 (40x lens).)

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GABARAP, Polyclonal Antibody (Cat# AAA30605)

Full Name

GABARAP Rabbit Polyclonal Antibody

Gene Names

GABARAP; MM46; ATG8A; GABARAP-a

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Affinity purification

Pricing

$360/0.05 mL $450/0.1 mL $855/2x0.1 mL $1,230/3x0.1 mL $1,595/4x0.1 mL $1,750/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using MAP2K5 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using MAP2K5 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using MAP2K5 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using MAP2K5 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat spinal cord using MAP2K5 Antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using MAP2K5 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 20s.)

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MAP2K5, Polyclonal Antibody (Cat# AAA28148)

Full Name

MAP2K5 Polyclonal Antibody

Gene Names

MAP2K5; MEK5; MAPKK5; PRKMK5; HsT17454

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of U251 cells using anti-GABARAP antibody (AAA19250).Overlay histogram showing U251 cells stained with AAA19250 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GABARAP Antibody (AAA19250, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of HEPA1-6 cells using anti-GABARAP antibody (AAA19250).Overlay histogram showing HEPA1-6 cells stained with AAA19250 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GABARAP Antibody (AAA19250, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of GABARAP using anti-GABARAP antibody (AAA19250).GABARAP was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-GABARAP Antibody (AAA19250) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of GABARAP using anti-GABARAP antibody (AAA19250).GABARAP was detected in paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-GABARAP Antibody (AAA19250) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of GABARAP using anti-GABARAP antibody (AAA19250).GABARAP was detected in paraffin-embedded section of human renal carcinomar tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-GABARAP Antibody (AAA19250) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of GABARAP using anti-GABARAP antibody (AAA19250).GABARAP was detected in paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-GABARAP Antibody (AAA19250) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of GABARAP using anti-GABARAP antibody (AAA19250).GABARAP was detected in paraffin-embedded section of human esophageal squamous carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-GABARAP Antibody (AAA19250) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of GABARAP using anti-GABARAP antibody (AAA19250).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HEK293 whole cell lysatesLane 2: human Jurkat whole cell lysatesLane 3: human A549 whole cell lysatesLane 4: human HELA whole cell lysatesLane 5: human U87 whole cell lysatesLane 6: human HEPG2 whole cell lysatesLane 7: human U937 whole cell lysatesLane 8: rat liver tissue lysatesLane 9: rat pancreas tissue lysatesLane 10: mouse liver tissue lysates, Lane 11: mouse pancreas tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-GABARAP antigen affinity purified polyclonal antibody (Catalog # AAA19250) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for GABARAP at approximately 18KD. The expected band size for GABARAP is at 18KD.)

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GABARAP, Polyclonal Antibody (Cat# AAA19250)

Full Name

Anti-GABARAP Antibody

Gene Names

GABARAP; MM46; ATG8A; GABARAP-a

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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IF (Immunofluorescence) (Immunofluorescence staining of KLH-SMCCA301 in C2C12 cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-mouse KLH-SMCCA301 polyclonal antibody (dilution ratio 1:100) at 4? overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Cytoplasm.)

IHC (Immunohistochemistry-Paraffin) (Immunochemical staining of rat in rat heart with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistchemistry-Paraffin) (Immunochemical staining of Mouse DES in Mouse stomach with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry-Paraffin) (Immunochemical staining of Mouse DES in Mouse heart with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry-Paraffin) (Immunochemical staining of human DES in human stomach with rabbit polyclonal antibody at 1:1000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry-Paraffin) (Immunochemical staining of human DES in human prostate with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry-Paraffin) (Immunochemical staining of human DES in human heart with rabbit polyclonal antibody at 1:100 dilution, formalin-fixed paraffin embedded sections.)

WB (Western Blot) (Anti-Desmin rabbit polyclonal antibody at 1:500 dilutionLane A: Mouse heart tissue lysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution.Developed using the ECL technique.Performed under reducing conditions.Predicted band size:53 kDaObserved band size:55 kDa)

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Desmin, Polyclonal Antibody (Cat# AAA27768)

Full Name

Anti-Desmin Antibody, Rabbit Polyclonal

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence

Purity

Protein A & Antigen Affinity

Pricing

$405/0.1 mL $760/2x0.1 mL $1,105/3x0.1 mL $1,420/4x0.1 mL $1,520/5x0.1 mL

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IHC (Immunohistchemistry) (Formalin-fixed and paraffin-embedded human Spleen tissue reacted with KLF4 antibody (N-term C74) , which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

IHC (Immunohistochemistry) (Formalin-fixed and paraffin-embedded human prostata carcinoma tissue reacted with KLF4 antibody (N-term C74) , which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

IF (Immunofluorescence) (Fluorescent confocal image of SY5Y cells stained with AAA28755 KLF4 (N-term C74) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with AAA28755 KLF4 (N-term C74) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 uM, 25 min). KLF4 immunoreactivity is localized to the nuclei in SY5Y cells.)

IF (Immunofluorescence) (Fluorescent confocal image of HeLa cells stained with AAA28755 KLF4 (N-term C74) antibody. HeLa cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min), then incubated with AAA28755 KLF4 (N-term C74) primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (5.25 uM, 25 min). KLF4 immunoreactivity is localized to the nuclei in HeLa cells.)

WB (Western Blot) (Western blot analysis of KLF4 Antibody (N-term C74) in mouse stomach tissue lysates (35ug/lane). KLF4 (arrow) was detected using the purified Pab.)

WB (Western Blot) (Western blot analysis of KLF4 Antibody (N-term C74) in Jurkat,293 cell line lysates (35ug/lane).KLF4 (arrow) was detected using the purified Pab.)

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KLF4, Polyclonal Antibody (Cat# AAA28755)

Full Name

KLF4 Antibody (N-term C74)

Gene Names

KLF4; EZF; GKLF

Reactivity

Human, mouse

Applications

Western Blot, Immunofluorescence, Immunohistochemistry

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$275/0.08 mL $510/0.4 mL $975/2x0.4 mL $1,415/3x0.4 mL $1,825/4x0.4 mL $2,070/5x0.4 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse spinal cord using RPN1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse testis using RPN1 antibody at dilution of 1:100 (20x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using RPN1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using RPN1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human tonsil using RPN1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using RPN1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using RPN1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon using RPN1 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using RPN1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

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RPN1, Polyclonal Antibody (Cat# AAA28249)

Full Name

RPN1 Polyclonal Antibody

Gene Names

RPN1; OST1; RBPH1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H2B-K15 antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using Acetyl-Histone H2B-K15 antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using Acetyl-Histone H2B-K15 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using H2B K15ac antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using H2B K15ac antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast cancer using H2B K15ac antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat lung using H2B K15ac antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using Acetyl-Histone H2B-K15 antibody.)

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Histone H2B-K15, Polyclonal Antibody (Cat# AAA29817)

Full Name

Acetyl-Histone H2B-K15 pAb

Reactivity

Human, Mouse, Rat, Other (Wide, Range)

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation

Purity

Affinity purification

Pricing

$360/0.05 mL $450/0.1 mL $855/2x0.1 mL $1,230/3x0.1 mL $1,595/4x0.1 mL $1,750/5x0.1 mL

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.