Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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IHC (Immunohistochemistry) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

IHC (Immunohistchemistry) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

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Abin-2, Polyclonal Antibody (Cat# AAA29374)

Full Name

Abin-2 Polyclonal Antibody

Gene Names

TNIP2; KLIP; ABIN2; FLIP1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

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IHC (Immunohistochemistry) (At 1/100 staining Human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human ovarian cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of MUC1 (Phospho-Ser1227) using 293 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

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CD227/MUC1, Polyclonal Antibody (Cat# AAA31426)

Full Name

Phospho-CD227/MUC1 (Ser1227) Antibody

Gene Names

MUC1; EMA; PEM; PUM; KL-6; MAM6; PEMT; CD227; H23AG; MUC-1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$345/0.05 mL $415/0.1 mL $485/0.2 mL $925/2x0.2 mL $1,340/3x0.2 mL $1,725/4x0.2 mL $1,900/5x0.2 mL

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IHC (Immunohistchemistry) (Figure 6. IHC analysis of PDPK1 using anti-PDPK1 antibody (AAA11643).PDPK1 was detected in frozen section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PDPK1 Antibody (AAA11643) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of PDPK1 using anti-PDPK1 antibody (AAA11643).PDPK1 was detected in frozen section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PDPK1 Antibody (AAA11643) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of PDPK1 using anti-PDPK1 antibody (AAA11643).PDPK1 was detected in paraffin-embedded section of Human Lung Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PDPK1 Antibody (AAA11643) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of PDPK1 using anti-PDPK1 antibody (AAA11643).PDPK1 was detected in paraffin-embedded section of Rat Testis Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PDPK1 Antibody (AAA11643) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of PDPK1 using anti-PDPK1 antibody (AAA11643).PDPK1 was detected in paraffin-embedded section of Mouse Intestine Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-PDPK1 Antibody (AAA11643) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of PDPK1 using anti-PDPK1 antibody (AAA11643).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Rat Liver Tissue Lysate,Lane 2: Rat Lung Tissue Lysate,Lane 3: Mouse Liver Tissue Lysate,Lane 4: Mouse Lung Tissue Lysate,Lane 5: COLO320 Whole Cell Lysate,Lane 6: MCF-7 Whole Cell Lysate.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PDPK1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PDPK1 at approximately 70KD. The expected band size for PDPK1 is at 63KD.)

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PDPK1, Polyclonal Antibody (Cat# AAA11643)

Full Name

Anti-PDPK1 Antibody

Gene Names

PDPK1; PDK1; PDPK2; PDPK2P; PRO0461

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Immunogen Affinity Purified

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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ICC (Immunocytochemistry) (Anti- MCM7 Picoband antibody, AAA11649,ICCICC: A549 Cell)

IHC (Immunohistochemistry) (Anti- MCM7 Picoband antibody, AAA11649,IHC(P)IHC(P): Human Lung Cancer Tissue)

IHC (Immunohistochemistry) (Anti- MCM7 Picoband antibody, AAA11649,IHC(P)IHC(P): Rat Intestine Tissue)

IHC (Immunohistochemistry) (Anti- MCM7 Picoband antibody, AAA11649,IHC(P)IHC(P): Mouse Testis Tissue)

WB (Western Blot) (Anti- MCM7 Picoband antibody, AAA11649, Western blottingAll lanes: Anti MCM7 (AAA11649) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Human Placenta Tissue Lysate at 50ugLane 3: NIH3T3 Whole Cell Lysate at 40ugLane 4: HELA Whole Cell Lysate at 40ugLane 5: JURKAT Whole Cell Lysate at 40ugLane 6: 22RV1 Whole Cell Lysate at 40ugLane 7: COLO320 Whole Cell Lysate at 40ugLane 8: PC-12 Whole Cell Lysate at 40ugPredicted bind size: 81KDObserved bind size: 81KD)

WB (Western Blot) (Anti- MCM7 Picoband antibody, AAA11649, Western blottingAll lanes: Anti MCM7 (AAA11649) at 0.5ug/mlWB: Recombinant Human MCM7 Protein 0.5ngPredicted bind size: 39KDObserved bind size: 39KD)

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MCM7, Polyclonal Antibody (Cat# AAA11649)

Full Name

Anti-MCM7 Antibody

Gene Names

MCM7; MCM2; CDC47; P85MCM; P1CDC47; PNAS146; PPP1R104; P1.1-MCM3

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Immunogen Affinity Purified

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human uterine cancer using USO1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using USO1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using USO1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human tonsil using USO1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human kidney using USO1 antibody at dilution of 1:200 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using USO1 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

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USO1, Polyclonal Antibody (Cat# AAA10760)

Full Name

USO1 Polyclonal Antibody

Gene Names

USO1; TAP; VDP; P115

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using TXNL1 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using TXNL1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat testis using TXNL1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using TXNL1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver injury using TXNL1 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using TXNL1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

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TXNL1, Polyclonal Antibody (Cat# AAA10784)

Full Name

TXNL1 Polyclonal Antibody

Gene Names

TXNL1; Txl; TXNL; TRP32; TXL-1; HEL-S-114

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of A-549 cells using Bcl-2 Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using Bcl-2 Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using Bcl-2 Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using Bcl-2 Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using Bcl-2 Polyclonal Antibody at dilution of 1:200. Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines using Bcl-2 Polyclonal Antibody at 1:1000 dilution.)

WB (Western Blot) (Western blot analysis of extracts of Mouse lung using Bcl-2 Polyclonal Antibody at 1:500 dilution.)

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Bcl-2, Polyclonal Antibody (Cat# AAA22304)

Full Name

Bcl-2 Polyclonal Antibody

Gene Names

BCL2; Bcl-2; PPP1R50

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunofluorescence

Purity

Affinity purification

Pricing

$325/0.06 mL $435/0.12 mL $655/0.2 mL $1,245/2x0.2 mL $1,810/3x0.2 mL $2,340/4x0.2 mL $2,855/5x0.2 mL

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IHC (Immunohistchemistry) (Immunohistochemistry Analysis: Representative lot data. (Fig. 1 and 2) Paraffin-embedded mouse and human brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:100 dilution. Reactivity was detected using the IHC-Select Detection Kit. Staining pattern appears as cytoplasmic. (Fig. 3 and 4) Paraffin-embedded mouse and mouse olfactory lobe and cerebellum brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a Chicken IgY Antibody 1:100 dilution of Cat. No. AB15894, anti-Tbr2. Reactivity was detected using the IHC-Select Detection Kit. Immunoreactivity seen here is mostly nuclear.)

IHC (Immunohistochemistry) (Paraffin-embedded mouse cerebellum was prepared using epitope retrieval in citrate buffer, pH 6.0. Staining was performed using 1:100 dilution. Immuno-reactivity is mostly nuclear.)

IHC (Immunohistochemistry) (Paraffin-embedded mouse olfactory lobe was prepared using epitope retrieval in citrate buffer, pH 6.0. Staining was performed using a 1:100 dilution. Reactivity is mostly nuclear.)

IS (Immunostaining) (Paraffin-embedded human brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:100 dilution. Staining pattern appears as cytoplasmic.)

IS (Immunostaining) (Paraffin-embedded mouse brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:100 dilution. Staining pattern appears as cytoplasmic.)

WB (Western Blot) (Western Blot Analysis: Representative lot data. E13-14 mouse brain lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with (1:10,000) dilution. Proteins were visualized using a Rabbit Anti-Chicken conjugated to HRP and a chemi-luminescence detection system. Arrow indicates Tbr2 (~73 kD).)

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EOMES, Polyclonal Antibody (Cat# AAA26887)

Full Name

EOMES (Eomesodermin Homolog, T-box Brain Protein 2, TBR2, T-brain-2, TBR-2)

Gene Names

Eomes; Tbr2; TBR-2; C77258

Reactivity

Mouse, Human, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified by affinity chromatography.

Pricing

$1,355/0.1 mL $2,610/2x0.1 mL $3,795/3x0.1 mL $4,920/4x0.1 mL $5,805/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry Analysis: Representative lot data. (Fig. 1 and 2) Paraffin-embedded mouse and human brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:100 dilution. Reactivity was detected using the IHC-Select Detection Kit. Staining pattern appears as cytoplasmic. (Fig. 3 and 4) Paraffin-embedded mouse and mouse olfactory lobe and cerebellum brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a Chicken IgY Antibody 1:100 dilution of Cat. No. AB15894, anti-Tbr2. Reactivity was detected using the IHC-Select Detection Kit. Immunoreactivity seen here is mostly nuclear.)

IHC (Immunohistochemistry) (Paraffin-embedded mouse cerebellum was prepared using epitope retrieval in citrate buffer, pH 6.0. Staining was performed using 1:100 dilution. Immuno-reactivity is mostly nuclear.)

IHC (Immunohistochemistry) (Paraffin-embedded mouse olfactory lobe was prepared using epitope retrieval in citrate buffer, pH 6.0. Staining was performed using a 1:100 dilution. Reactivity is mostly nuclear.)

IS (Immunostaining) (Paraffin-embedded human brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:100 dilution. Staining pattern appears as cytoplasmic.)

IS (Immunostaining) (Paraffin-embedded mouse brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:100 dilution. Staining pattern appears as cytoplasmic.)

WB (Western Blot) (Western Blot Analysis: Representative lot data. E13-14 mouse brain lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with (1:10,000) dilution. Proteins were visualized using a Rabbit Anti-Chicken conjugated to HRP and a chemi-luminescence detection system. Arrow indicates Tbr2 (~73 kD).)

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EOMES, Polyclonal Antibody (Cat# AAA26888)

Full Name

EOMES (Eomesodermin Homolog, T-box Brain Protein 2, TBR2, T-brain-2, TBR-2)

Gene Names

Eomes; Tbr2; TBR-2; C77258

Reactivity

Mouse, Human, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified by affinity chromatography.

Pricing

$1,260/0.1 mL $2,440/2x0.1 mL $3,545/3x0.1 mL $4,590/4x0.1 mL $5,395/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse heart using ATP5B antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using ATP5B antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using ATP5B antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using ATP5B antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat heart using ATP5B antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver injury using ATP5B antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using ATP5B Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 3s.)

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ATP5B, Polyclonal Antibody (Cat# AAA28083)

Full Name

ATP5B Polyclonal Antibody

Gene Names

ATP5B; ATPMB; ATPSB; HEL-S-271

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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WB (Western Blot) (Anti-GP73 rabbit polyclonal antibody at 1:500 dilution Lane A: GOLM1 konckout Hela Whole Cell Lysate Lane B: Hela Whole Cell Lysate Lysates/proteins at 10 ug per lane. Secondary Goat Anti-Rabbit IgG (H+L)/HRP at 1/10000 dilution. Developed using the ECL technique. Performed under reducing conditions. Predicted band size:45 kDa Observed band size: kDa)

IF (Immunofluorescence) (Immunofluorescence staining of GP73 in HeLa cells. Cells were fixed with 4% PFA,blocked with 10% serum, and incubated with rabbit anti-human GP73 polyclonal antibody (dilution ratio 1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red) and counterstained with DAPI (blue). Positive staining was localized to cytoplasm.)

WB (Western Blot) (Anti-GP73 rabbit polyclonal antibody at 1:500 dilutionLane A: Jurkat Whole Cell LysateLane B: 293T Whole Cell LysateLane C: Hela Whole Cell LysateLane D: A549 Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:45 kDaObserved band size:72 kDa(We are unsure as to the identity of these extra bands.))

IP (Immunoprecipitation) (GP73 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell Lysate2 uL anti-GP73 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-GP73 rabbit polyclonal antibody,at 1:200 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 45 kDaObserved band size: 70 kDa)

IHC (Immunohistochemistry) (Immunochemical staining of human GP73 in human hepatoma with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).)

IHC (Immunohistochemistry) (Immunochemical staining of human GP73 in human liver with rabbit polyclonal antibody (1:5000, formalin-fixed paraffin embedded sections).)

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GOLPH2/GOLM1, Polyclonal Antibody (Cat# AAA27751)

Full Name

Anti-GOLPH2/GOLM1 Antibody, Rabbit Polyclonal

Gene Names

GOLM1; GP73; HEL46; GOLPH2; C9orf155; PSEC0257; bA379P1.3

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

Purity

Protein A & Antigen Affinity

Pricing

$405/0.1 mL $760/2x0.1 mL $1,105/3x0.1 mL $1,420/4x0.1 mL $1,520/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat testis using PSMC6 antibody at dilution of 1:200 (200x lens).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast cancer using PSMC6 antibody at dilution of 1:200 (200x lens).)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human endometrial cancer using PSMC6 antibody at dilution of 1:200 (200x lens).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney cancer using PSMC6 antibody at dilution of 1:200 (200x lens).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human stomach cancer using PSMC6 antibody at dilution of 1:200 (200x lens).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human stomach using PSMC6 antibody at dilution of 1:200 (200x lens).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human esophagus using PSMC6 antibody at dilution of 1:200 (200x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using PSMC6 antibody.)

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PSMC6, Polyclonal Antibody (Cat# AAA29708)

Full Name

PSMC6 Antibody

Gene Names

PSMC6; P44; p42; SUG2; CADP44; HEL-S-73

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Antibodies were purified by affinity purification using immunogen.

Pricing

$360/0.05 mL $450/0.1 mL $855/2x0.1 mL $1,230/3x0.1 mL $1,595/4x0.1 mL $1,750/5x0.1 mL

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IHC (Immunohistchemistry) (Dilution: WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry) (Dilution: WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000)

WB (Western Blot) (Dilution: WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry-Paraffin) (Dilution: WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry-Paraffin) (Dilution: WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000)

WB (Western Blot) (Dilution: WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000)

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NFAT5, Polyclonal Antibody (Cat# AAA29345)

Full Name

NFAT5 Polyclonal Antibody

Gene Names

NFAT5; NFATZ; OREBP; NF-AT5; NFATL1; TONEBP

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

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IHC (Immunohistchemistry) (Dilution: WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry) (Dilution: WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000)

WB (Western Blot) (Dilution: WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry-Paraffin) (Dilution: WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry-Paraffin) (Dilution: WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000)

WB (Western Blot) (Dilution: WB 1:500-2000,IHC-p 1:500-200, ELISA 1:10000-20000)

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CKR-5, Polyclonal Antibody (Cat# AAA29347)

Full Name

CKR-5 Polyclonal Antibody

Gene Names

CCR5; CKR5; CCR-5; CD195; CKR-5; CCCKR5; CMKBR5; IDDM22; CC-CKR-5

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

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Application Data (At 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/200 staining Human kidney cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/200 staining Mouse intestinal tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/200 staining Rat lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of Retinoblastoma (Phospho-Ser788) using K562 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

WB (Western Blot) (Western blot analysis of extracts from Mouse heart, using Phospho-Retinoblastoma (Ser788) Antibody. The lane on the left was treated with blocking peptide.Observed bands: 125kDa)

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Retinoblastoma, Polyclonal Antibody (Cat# AAA31401)

Full Name

Phospho-Retinoblastoma (Ser788) Antibody

Gene Names

RB1; RB; pRb; OSRC; pp110; p105-Rb

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (100%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (92%), Xenopus (92%)

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$345/0.05 mL $415/0.1 mL $485/0.2 mL $925/2x0.2 mL $1,340/3x0.2 mL $1,725/4x0.2 mL $1,900/5x0.2 mL

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using ZNF581 antibody at dilution of 1:100 .)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using ZNF581 antibody at dilution of 1:100 .)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat pancreas using ZNF581 antibody at dilution of 1:100 .)

IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using ZNF581 Polyclonal Antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using ZNF581 Polyclonal Antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using ZNF581 Polyclonal Antibody at dilution of 1:100 . Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using ZNF581 antibody at 1:3000 dilution.)

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ZNF581, Polyclonal Antibody (Cat# AAA30560)

Full Name

ZNF581 Polyclonal Antibody

Gene Names

ZNF581; HSPC189

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

$360/0.05 mL $450/0.1 mL $855/2x0.1 mL $1,230/3x0.1 mL $1,595/4x0.1 mL $1,750/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat spleen using MMP9 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse spleen using MMP9 at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human liver using MMP9 at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using MMP9 at 1:1000 dilution.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human stomach using MMP9 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human esophagus using MMP9 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver damage using MMP9 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using MMP9 at dilution of 1:100 (40x lens).)

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MMP9, Polyclonal Antibody (Cat# AAA30591)

Full Name

MMP9 Rabbit Polyclonal Antibody

Gene Names

MMP9; GELB; CLG4B; MMP-9; MANDP2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Affinity purification

Pricing

$360/0.05 mL $450/0.1 mL $855/2x0.1 mL $1,230/3x0.1 mL $1,595/4x0.1 mL $1,750/5x0.1 mL

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of HL-60 cells using anti-PAP/ACP3 antibody (AAA19446).Overlay histogram showing HL-60 cells stained with AAA19446 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PAP/ACP3 Antibody (AAA19446, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of PAP/ACP3 using anti-PAP/ACP3 antibody (AAA19446).PAP/ACP3 was detected in an immunocytochemical section of U87 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-PAP/ACP3 Antibody (AAA19446) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of PAP/ACP3 using anti-PAP/ACP3 antibody (AAA19446).PAP/ACP3 was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-PAP/ACP3 Antibody (AAA19446) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of PAP/ACP3 using anti-PAP/ACP3 antibody (AAA19446).PAP/ACP3 was detected in a paraffin-embedded section of mouse kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-PAP/ACP3 Antibody (AAA19446) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of PAP/ACP3 using anti-PAP/ACP3 antibody (AAA19446).PAP/ACP3 was detected in a paraffin-embedded section of human prostate cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-PAP/ACP3 Antibody (AAA19446) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of PAP/ACP3 using anti-PAP/ACP3 antibody (AAA19446).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human MCF-7 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAP/ACP3 antigen affinity purified polyclonal antibody (#AAA19446) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PAP/ACP3 at approximately 45-50 kDa. The expected band size for PAP/ACP3 is at 45 kDa.)

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PAP/ACP3, Polyclonal Antibody (Cat# AAA19446)

Full Name

Anti-PAP/ACP3 Antibody Picoband

Gene Names

ACPP; ACP3; 5'-NT; ACP-3

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ICC, IF, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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WB (Western Blot) (Host: RabbitTarget: GATA2Positive control (+): Hela (HL)Negative control (-): 293T (2T)Antibody concentration: 1ug/ml)

WB (Western Blot) (WB Suggested Anti-GATA2 Antibody Titration: 1.0-2.0ug/mlELISA Titer: 1:1562500Positive Control: K562 cell lysateGATA2 is strongly supported by BioGPS gene expression data to be expressed in Human K562 cells)

WB (Western Blot) (WB Suggested Anti-GATA2 antibody Titration: 1 ug/mLSample Type: Human K562)

WB (Western Blot) (Host: RabbitTarget Name: GATA2Sample Tissue: Human HepG2 Whole CellAntibody Dilution: 1ug/ml)

WB (Western Blot) (Sample Type: Mouse liver, N2aLanes :Lane 1: 30ug mouse liver lysateLane 2: 30ug mouse N2a cell lysatePrimary Antibody Dilution :1:500Secondary Antibody :Anti-rabbit-HRPSecondary Antibody Dilution :1:2500Gene Name :GATA2Submitted by :A Kalyani and Vinayak Gupta IIT Madras)

IHC (Immunohistochemistry) (Human Liver)

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GATA2, Polyclonal Antibody (Cat# AAA23392)

Full Name

GATA2 antibody - N-terminal region

Gene Names

GATA2; DCML; IMD21; NFE1B; MONOMAC

Reactivity

Tested Reactivity: Human, Mouse

Predicted Reactivity: Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Protein A purified

Pricing

$475/0.1 mL $905/2x0.1 mL $1,310/3x0.1 mL $1,690/4x0.1 mL $1,890/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemistry-CLNS1A Polyclonal Antibody)

IHC (Immunohistchemistry) (Immunohistochemistry-CLNS1A Polyclonal Antibody)

IHC (Immunohistochemistry) (Immunohistochemistry-CLNS1A Polyclonal Antibody)

IHC (Immunohistochemistry) (Immunohistochemistry-CLNS1A Polyclonal Antibody)

IHC (Immunohistochemistry) (Immunohistochemistry-CLNS1A Polyclonal Antibody)

IHC (Immunohistochemistry) (Immunohistochemistry-CLNS1A Polyclonal Antibody)

WB (Western Blot) (Western blot-CLNS1A Polyclonal Antibody)

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CLNS1A, Polyclonal Antibody (Cat# AAA28292)

Full Name

CLNS1A Polyclonal Antibody

Gene Names

CLNS1A; CLCI; ICln; CLNS1B

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human stomach cancer using Bid antibody at dilution of 1:200 (200x lens).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human thyroid cancer using BID antibody at dilution of 1:200 (200x lens).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung cancer using BID antibody at dilution of 1:200 (200x lens).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain using BID antibody at dilution of 1:200 (200x lens).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Colon cancer using BID antibody.)

WB (Western Blot) (Western blot analysis of extracts of HL60 cell lines, using BID antibody.)

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BID, Polyclonal Antibody (Cat# AAA29655)

Full Name

BID Antibody

Gene Names

BID; FP497

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Antibodies were purified by affinity purification using immunogen.

Pricing

$360/0.05 mL $450/0.1 mL $855/2x0.1 mL $1,230/3x0.1 mL $1,595/4x0.1 mL $1,750/5x0.1 mL

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Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI (blue).)

IHC (Immunohistchemistry) (At 1/100 staining Human pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human kidney cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human ovarian cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

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RPA32/RPA2, Polyclonal Antibody (Cat# AAA31304)

Full Name

Phospho-RPA32/RPA2 (Ser4/Ser8) Antibody

Gene Names

RPA2; REPA2; RPA32

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$415/0.1 mL $485/0.2 mL $925/2x0.2 mL $1,340/3x0.2 mL $1,725/4x0.2 mL $1,900/5x0.2 mL

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using CHRNA1 antibody. Blue: DAPI for nuclear staining.)

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CHRNA1, Polyclonal Antibody (Cat# AAA28261)

Full Name

CHRNA1 Polyclonal Antibody

Gene Names

CHRNA1; ACHRA; ACHRD; CHRNA; CMS1A; CMS1B; CMS2A; FCCMS; SCCMS

Reactivity

Human

Applications

Immunofluorescence

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 100ug extracts of 293T cells using 3ug DDX1 antibody. Western blot was performed from the immunoprecipitate using DDX1 antibody at a dilition of 1:1000.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using DDX1 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using DDX1 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using DDX1 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse lung using DDX1 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using DDX1 Antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using DDX1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)

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DDX1, Polyclonal Antibody (Cat# AAA28165)

Full Name

DDX1 Polyclonal Antibody

Gene Names

DDX1; DBP-RB; UKVH5d

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

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BMAL1, Polyclonal Antibody (Cat# AAA29252)

Full Name

BMAL1 Polyclonal Antibody

Gene Names

ARNTL; TIC; JAP3; MOP3; BMAL1; PASD3; BMAL1c; bHLHe5

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using ZNF433 Polyclonal Antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using ZNF433 Polyclonal Antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using ZNF433 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using ZNF433 antibody.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse lung using ZNF433 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human uterine cancer using ZNF433 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using ZNF433 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human appendix using ZNF433 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using ZNF433 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using ZNF433 antibody.)

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ZNF433, Polyclonal Antibody (Cat# AAA29815)

Full Name

ZNF433 Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

$360/0.05 mL $450/0.1 mL $855/2x0.1 mL $1,230/3x0.1 mL $1,595/4x0.1 mL $1,750/5x0.1 mL

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of U-87 cells using anti-SSH3BP1 antibody (AAA11656).Overlay histogram showing U-87 cells stained with AAA11656 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SSH3BP1 Antibody (AAA11656,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

FCM (Flow Cytometry) (Figure 5. Flow Cytometry analysis of Neuro-2a cells using anti-SSH3BP1 antibody (AAA11656).Overlay histogram showing Neuro-2a cells stained with AAA11656 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SSH3BP1 Antibody (AAA11656,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

FCM (Flow Cytometry) (Figure 4. Flow Cytometry analysis of U937 cells using anti-SSH3BP1 antibody (AAA11656).Overlay histogram showing U937 cells stained with AAA11656 (Blue line).The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SSH3BP1 Antibody (AAA11656,1ug/1x10^6 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10ug/1x10^6 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of SSH3BP1 using anti-SSH3BP1 antibody (AAA11656). SSH3BP1 was detected in paraffin-embedded section of Rat Brain Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SSH3BP1 Antibody (AAA11656) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of SSH3BP1 using anti-SSH3BP1 antibody (AAA11656). SSH3BP1 was detected in paraffin-embedded section of Mouse Brain Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-SSH3BP1 Antibody (AAA11656) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of SSH3BP1 using anti-SSH3BP1 antibody (AAA11656). Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions. Lane 1: Rat Liver Tissue Lysate Lane 2: Rat Testis Tissue Lysate Lane 3: HELA Whole Cell Lysate Lane 4: RH35 Whole Cell Lysate Lane 5: HEPA Whole Cell Lysate After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SSH3BP1 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SSH3BP1 at approximately 55KD. The expected band size for SSH3BP1 is at 55KD.)

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SSH3BP1, Polyclonal Antibody (Cat# AAA11656)

Full Name

Anti-SSH3BP1 Antibody

Gene Names

ABI1; E3B1; ABI-1; ABLBP4; NAP1BP; SSH3BP; SSH3BP1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Immunogen Affinity Purified

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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IF (Immunofluorescence) (Figure 7 Immunofluorescence Validation of PEX3 in Rat testisImmunofluorescent analysis of 4% paraformaldehyde-fixed rat testis labeling PEX3 with AAA11047 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)

IF (Immunofluorescence) (Figure 6 Immunofluorescence Validation of PEX3 in Mouse ThymusImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse thymus labeling PEX3 with AAA11047 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)

IF (Immunofluorescence) (Figure 5 Immunofluorescence Validation of PEX3 in Human SpleenImmunofluorescent analysis of 4% paraformaldehyde-fixed human spleen labeling PEX3 with AAA11047 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)

IF (Immunofluorescence) (Figure 4 Immunofluorescence Validation of PEX3 in Human HeLa CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed HeLa cells labeling PEX3 with AAA11047 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)

WB (Western Blot) (Figure 3 Western Blot Validation in Rat TissuesLoading: 15ug of lysates per lane.Antibodies: PEX3 AAA11047, 2ug/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)

WB (Western Blot) (Figure 2 Western Blot Validation in Mouse Tissues Loading: 15ug of lysates per lane.Antibodies: PEX3 AAA11047, 2ug/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)

WB (Western Blot) (Figure 1 WB Validation in Human Cell Lines Loading: 15ug of lysate Antibodies: PEX3 AAA11047, 2ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.)

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PEX3, Polyclonal Antibody (Cat# AAA11047)

Full Name

PEX3 (CT) Antibody

Gene Names

PEX3; TRG18; PBD10A

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunofluorescence

Purity

PEX3 Antibody is affinity chromatography purified via peptide column.

Pricing

$570/0.1 mg $1,080/2x0.1 mg $1,560/3x0.1 mg $2,015/4x0.1 mg $2,295/5x0.1 mg

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FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of JK cells using anti-LGI4 antibody (AAA19971).Overlay histogram showing JK cells stained with AAA19971 (Blue line). The cells were fixed with 4% paraformaldehyde and blocked with 10% normal goat serum. And then incubated with rabbit anti-LGI4 Antibody (AAA19971, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 7. IF analysis of LGI4 using anti-LGI4 antibody (AAA19971).LGI4 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LGI4 Antibody (AAA19971) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LGI4 Antibody (AAA19971) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LGI4 Antibody (AAA19971) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LGI4 Antibody (AAA19971) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-LGI4 Antibody (AAA19971) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human Hacat whole cell lysates,Lane 3: human SiHa whole cell lysates,Lane 4: human U251 whole cell lysates,Lane 5: rat brain tissue lysates,Lane 6: rat stomach tissue lysates,Lane 7: mouse brain tissue lysates,Lane 8: mouse stomach tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LGI4 antigen affinity purified polyclonal antibody (#AAA19971) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LGI4 at approximately 59 kDa. The expected band size for LGI4 is at 59 kDa.)

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LGI4, Polyclonal Antibody (Cat# AAA19971)

Full Name

Anti-LGI4 Antibody Picoband

Gene Names

LGI4; LGIL3

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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WB (Western Blot) (Anti-HDAC3 antibody, AAA11582, Western blottingLane 1: Rat Stomach Tissue LysateLane 2: Rat Testis Tissue LysateLane 3: MCF-7 Cell LysateLane 4: HELA Cell LysateLane 5: JURKAT Cell LysateLane 6: SKOV Cell Lysate)

ICC (Immunocytochemistry) (Anti-HDAC3 antibody, AAA11582, ICCICC: A549 Cell)

IHC (Immunohistochemistry) (Anti-HDAC3 antibody, AAA11582, IHC(F)IHC(F): Mouse Brain Tissue)

IHC (Immunohistochemistry) (Anti-HDAC3 antibody, AAA11582, IHC(P)IHC(P): Rat Intestine Tissue)

IHC (Immunohistochemistry) (Anti-HDAC3 antibody, AAA11582, IHC(P)IHC(P): Human Lung Cancer Tissue)

IHC (Immunohistochemistry) (Anti-HDAC3 antibody, AAA11582, IHC(P)IHC(P): Human Intestinal Cancer Tissue)

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HDAC3, Polyclonal Antibody (Cat# AAA11582)

Full Name

Anti-HDAC3 antibody

Gene Names

HDAC3; HD3; RPD3; RPD3-2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunohistochemistry, Immunocytochemistry

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using SIAH1 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse spleen using SIAH1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse brain using SIAH1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast cancer using SIAH1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using SIAH1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat spleen using SIAH1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using SIAH1 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of mouse kidney, using SIAH1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

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SIAH1, Polyclonal Antibody (Cat# AAA10680)

Full Name

SIAH1 Polyclonal Antibody

Gene Names

SIAH1; SIAH1A

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of U87 cells using anti-RAB13 antibody (AAA19513).Overlay histogram showing U87 cells stained with AAA19513 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RAB13 Antibody (AAA19513, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 9. IF analysis of RAB13 using anti-RAB13 antibody (AAA19513).RAB13 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-RAB13 Antibody (AAA19513) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 8. IHC analysis of RAB13 using anti-RAB13 antibody (AAA19513).RAB13 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-RAB13 Antibody (AAA19513) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of RAB13 using anti-RAB13 antibody (AAA19513).RAB13 was detected in a paraffin-embedded section of human stomach cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-RAB13 Antibody (AAA19513) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of RAB13 using anti-RAB13 antibody (AAA19513).RAB13 was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-RAB13 Antibody (AAA19513) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of RAB13 using anti-RAB13 antibody (AAA19513).RAB13 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-RAB13 Antibody (AAA19513) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of RAB13 using anti-RAB13 antibody (AAA19513).RAB13 was detected in a paraffin-embedded section of human cervical cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-RAB13 Antibody (AAA19513) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of RAB13 using anti-RAB13 antibody (AAA19513).RAB13 was detected in a paraffin-embedded section of human adenocarcinoma of the right colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-RAB13 Antibody (AAA19513) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of RAB13 using anti-RAB13 antibody (AAA19513).RAB13 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-RAB13 Antibody (AAA19513) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of RAB13 using anti-RAB13 antibody (AAA19513).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human Caco-2 whole cell lysates,Lane 3: human MCF-7 whole cell lysates,Lane 4: human 293T whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RAB13 antigen affinity purified polyclonal antibody (#AAA19513) at 0.25 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RAB13 at approximately 23 kDa. The expected band size for RAB13 is at 23 kDa.)

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RAB13, Polyclonal Antibody (Cat# AAA19513)

Full Name

Anti-RAB13 Antibody Picoband

Gene Names

RAB13; GIG4

Reactivity

Human, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of PC-3 cells using anti-PAK3 antibody (AAA19477).Overlay histogram showing PC-3 cells stained with AAA19477 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PAK3 Antibody (AAA19477, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of PAK3 using anti-PAK3 antibody (AAA19477).PAK3 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-PAK3 Antibody (AAA19477) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of PAK3 using anti-PAK3 antibody (AAA19477).PAK3 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-PAK3 Antibody (AAA19477) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of PAK3 using anti-PAK3 antibody (AAA19477).PAK3 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-PAK3 Antibody (AAA19477) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of PAK3 using anti-PAK3 antibody (AAA19477).PAK3 was detected in a paraffin-embedded section of human thyroid papillary carcinom tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-PAK3 Antibody (AAA19477) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of PAK3 using anti-PAK3 antibody (AAA19477).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human placenta tissue lysates,Lane 2: human PANC-1 whole cell lysates,Lane 3: human U20S whole cell lysates,Lane 4: human PC-3 whole cell lysates,Lane 5: rat brain tissue lysates,Lane 6: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PAK3 antigen affinity purified polyclonal antibody (#AAA19477) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PAK3 at approximately 62 kDa. The expected band size for PAK3 is at 62 kDa.)

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PAK3, Polyclonal Antibody (Cat# AAA19477)

Full Name

Anti-PAK3 Antibody Picoband

Gene Names

PAK3; bPAK; MRX30; MRX47; OPHN3; hPAK3; CDKN1A; PAK3beta

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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WB (Western Blot) (WB Suggested Anti-UQCRQ AntibodyTitration: 1.0 ug/mlPositive Control: Fetal Liver)

WB (Western Blot) (Host: RabbitTarget Name: UQCRQSample Type: Human MCF7Antibody Dilution: 1.0ug/mlUQCRQ is supported by BioGPS gene expression data to be expressed in MCF7)

WB (Western Blot) (Host: RabbitTarget Name: UQCRQSample Type: Human HelaAntibody Dilution: 1.0ug/mlUQCRQ is supported by BioGPS gene expression data to be expressed in HeLa)

WB (Western Blot) (Host: RabbitTarget Name: UQCRQSample Type: Human Fetal KidneyAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: UQCRQSample Type: Human Fetal HeartAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: UQCRQSample Type: Human Fetal BrainAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: UQCRQSample Type: Human Adult PlacentaAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: UQCRQSample Type: Human 721_BAntibody Dilution: 1.0ug/mlUQCRQ is supported by BioGPS gene expression data to be expressed in 721_B)

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UQCRQ, Polyclonal Antibody (Cat# AAA23598)

Full Name

UQCRQ Antibody - middle region

Gene Names

UQCRQ; QPC; QCR8; QP-C; UQCR7; MC3DN4

Reactivity

Cow, Dog, Guinea Pig, Human, Mouse, Pig, Rabbit, Rat

Applications

Western Blot

Purity

Affinity Purified

Pricing

$530/0.1 mL $1,005/2x0.1 mL $1,455/3x0.1 mL $1,885/4x0.1 mL $2,140/5x0.1 mL

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IHC (Immunohistochemistry) (Immunochemical staining HMGB1 in rat liver with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

IP (Immunoprecipitation) (HMGB1 was immunoprecipitated using:Lane A:0.5 mg Hela Whole Cell LysateLane B:0.5 mg Jurkat Whole Cell LysateLane C:0.5 mg HepG2 Whole Cell Lysate4 uL anti-HMGB1 rabbit polyclonal antibody and 15 ul of 50 % Protein G agarose.Primary antibody:Anti-HMGB1 rabbit polyclonal antibody,at 1:100 dilution Secondary antibody:Dylight 800-labeled antibody to rabbit IgG (H+L), at 1:5000 dilution Developed using the odssey technique.Performed under reducing conditions.Predicted band size: 25 kDaObserved band size: 25 kDa)

IF (Immunofluorescence) (Immunofluorescence staining of HMGB1 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human HMGB1 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue). Positive staining was localized to nucleus.)

IHC (Immunohistochemistry) (Immunochemical staining HMGB1 in rat kidney with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

IHC (Immunohistochemistry) (Immunochemical staining HMGB1 in mouse kidney with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

IHC (Immunohistochemistry) (Immunochemical staining HMGB1 in human liver with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

IHC (Immunohistochemistry) (Immunochemical staining HMGB1 in human kidney with rabbit polyclonal antibody (1:1000, formalin-fixed paraffin embedded sections).)

WB (Western Blot) (Anti-HMGB1 rabbit polyclonal antibody at 1:500 dilutionLane A: HeLa Whole Cell LysateLane B: HepG2 Whole Cell LysateLane C: Jurkat Whole Cell LysateLysates/proteins at 30 ug per lane.SecondaryGoat Anti- Rabbit  IgG H&L (Dylight 800)  at 1/10000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.Predicted band size:25 kDaObserved band size:25 kDa)

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HMGB1, Polyclonal Antibody (Cat# AAA27719)

Full Name

Anti-HMGB1 Antibody, Rabbit Polyclonal

Gene Names

HMGB1; HMG1; HMG3; SBP-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Immunoprecipitation

Purity

Protein A & Antigen Affinity

Pricing

$205/0.02 mL $405/0.1 mL $760/2x0.1 mL $1,105/3x0.1 mL $1,420/4x0.1 mL $1,520/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using TSC22D3 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using TSC22D3 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human stomach using TSC22D3 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using TSC22D3 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse lung using TSC22D3 Antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using TSC22D3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

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TSC22D3, Polyclonal Antibody (Cat# AAA28179)

Full Name

TSC22D3 Polyclonal Antibody

Gene Names

TSC22D3; DIP; GILZ; hDIP; DSIPI; TSC-22R

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

WB (Western Blot) (Western blot analysis of extracts from LOVO cells and RAW264.7 cells, using CEBPE antibody.)

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CEBPE, Polyclonal Antibody (Cat# AAA29704)

Full Name

CEBPE Antibody

Gene Names

CEBPD; CELF; CRP3; C/EBP-delta; NF-IL6-beta

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$305/0.05 mL $365/0.1 mL $680/2x0.1 mL $980/3x0.1 mL $1,260/4x0.1 mL $1,355/5x0.1 mL

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Application Data (At 25 degree C. Samples were then incubated with primary Ab(At 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)

IHC (Immunohistochemistry) (At 1/100 staining Human pancreatic cancer and adjacent nomal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Human pancreatic cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human kidney cancer and adjacent normal tissues by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Human ovarian cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of PDX1 (Phospho-Ser66) using EGF treated A431 whole cell lysates.-/+ means absence or presence of N peptide（non-phospho peptide) and P peptide(phospho peptide).)

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PDX1, Polyclonal Antibody (Cat# AAA31430)

Full Name

Phospho-PDX1 (Ser66) Antibody

Gene Names

PDX1; GSF; IPF1; IUF1; IDX-1; MODY4; PDX-1; STF-1

Reactivity

Human, Mouse, Rat
Predicted Reactivity: Pig (88%), Bovine (100%), Horse (100%), Sheep (100%), Rabbit (100%), Dog (100%), Chicken (88%), Xenopus (100%)

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$345/0.05 mL $415/0.1 mL $485/0.2 mL $925/2x0.2 mL $1,340/3x0.2 mL $1,725/4x0.2 mL $1,900/5x0.2 mL

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000.IHC-p:1:50-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000.IHC-p:1:50-300 ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000.IHC-p:1:50-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000.IHC-p:1:50-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000.IHC-p:1:50-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000.IHC-p:1:50-300 ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000.IHC-p:1:50-300 ELISA: 1/20000. Not yet tested in other applications.)

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IL-12A, Polyclonal Antibody (Cat# AAA29047)

Full Name

IL-12A Polyclonal Antibody

Gene Names

IL12A; P35; CLMF; NFSK; NKSF1; IL-12A

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: WB 1:500-2000, IF 1:50-300, IHC 1:50-300)

IHC (Immunohistchemistry) (Dilution: WB 1:500-2000, IF 1:50-300, IHC 1:50-300)

IHC (Immunohistochemistry) (Dilution: WB 1:500-2000, IF 1:50-300, IHC 1:50-300)

IHC (Immunohistochemistry-Paraffin) (Dilution: WB 1:500-2000, IF 1:50-300, IHC 1:50-300)

IHC (Immunohistochemistry-Paraffin) (Dilution: WB 1:500-2000, IF 1:50-300, IHC 1:50-300)

IHC (Immunohistochemistry-Paraffin) (Dilution: WB 1:500-2000, IF 1:50-300, IHC 1:50-300)

WB (Western Blot) (Dilution: WB 1:500-2000, IF 1:50-300, IHC 1:50-300)

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Cleaved-PARP-1, Polyclonal Antibody (Cat# AAA28838)

Full Name

Cleaved-PARP-1 (D214) Polyclonal Antibody

Gene Names

PARP1; PARP; PPOL; ADPRT; ARTD1; ADPRT1; PARP-1; ADPRT 1; pADPRT-1

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts of various cell lines, using NAA10 at 1:1000 dilution.)

IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using NAA10. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse lung using NAA10 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human stomach using NAA10 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast cancer using NAA10 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat lung using NAA10 at dilution of 1:100 (40x lens).)

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NAA10, Polyclonal Antibody (Cat# AAA30685)

Full Name

NAA10 Rabbit Polyclonal Antibody

Gene Names

NAA10; TE2; ARD1; NATD; ARD1A; ARD1P; DXS707; MCOPS1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

$360/0.05 mL $450/0.1 mL $855/2x0.1 mL $1,230/3x0.1 mL $1,595/4x0.1 mL $1,750/5x0.1 mL

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IHC (Immunohistochemistry) (Immunochemical staining RBBP4 in mouse lung with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistchemistry) (Immunochemical staining RBBP4 in mouse kidney with rabbit polyclonal antibody at 1:2000 dilution, formalin-fixed paraffin embedded sections.)

WB (Western Blot) (Anti-RBBP4 rabbit polyclonal antibody at 1:500 dilution. Lane A: HepG2 Whole Cell Lysate Lane B: K562 Whole Cell Lysate Lane C: Mouse spleen tissue lysate Lane D: Rat lung tissue lysate Lysates/proteins at 30 ug per lane. Secondary Goat Anti-Rabbit IgG H&L (Dylight800) at 1/10000 dilution. Developed using the Odyssey technique. Performed under reducing conditions. Predicted band size:48 kDa Observed band size:55 kDa)

IHC (Immunohistochemistry) (Immunochemical staining RBBP4 in human breast carcinoma with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)

IHC (Immunohistochemistry) (Immunochemical staining RBBP4 in human lung cancer with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)

IHC (Immunohistochemistry) (Immunochemical staining RBBP4 in human testis with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)

IF (Immunofluorescence) (Immunofluorescence staining of RBBP4 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.3% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human RBBP4 polyclonal antibody (1:1000) at 4 degree C overnight. Then cells were stained with the Alexa Fluor594-conjugated Goat Anti-rabbit IgG secondary antibody (red)Positive staining was localized to nucleus.)

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RbAp48, Polyclonal Antibody (Cat# AAA27724)

Full Name

Anti-RbAp48 Antibody, Rabbit Polyclonal

Gene Names

RBBP4; NURF55; RBAP48; lin-53

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence

Purity

Protein A & Antigen Affinity

Pricing

$405/0.1 mL $760/2x0.1 mL $1,105/3x0.1 mL $1,420/4x0.1 mL $1,520/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of SK-Br-3 cells using PTAR1 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohistochemistry) (Formalin-fixed and paraffin-embedded human breast carcinoma with PTAR1 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

WB (Western Blot) (Western blot analysis of PTAR1 Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane). PTAR1 (arrow) was detected using the purified Pab.)

WB (Western Blot) (Western blot analysis of PTAR1 Antibody (Center) in Jurkat cell line lysates (35ug/lane). PTAR1 (arrow) was detected using the purified Pab.)

WB (Western Blot) (Western blot analysis of PTAR1 Antibody (Center) in HL-60 cell line lysates (35ug/lane). PTAR1 (arrow) was detected using the purified Pab.)

WB (Western Blot) (Western blot analysis of PTAR1 Antibody (Center) in CEM cell line lysates (35ug/lane). PTAR1 (arrow) was detected using the purified Pab.)

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PTAR1, Polyclonal Antibody (Cat# AAA28765)

Full Name

PTAR1 Antibody (Center)

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$275/0.08 mL $510/0.4 mL $975/2x0.4 mL $1,415/3x0.4 mL $1,825/4x0.4 mL $2,070/5x0.4 mL

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WB (Western Blot) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot) (Western blot analysis of anti-PACSIN1 Pab in mouse brain tissue lysate. PACSIN1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

WB (Western Blot) (Western blot analysis of anti-PACSIN1 Pab in HL-60 cell line lysate. PACSIN1 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

WB (Western Blot) (Western blot analysis of lysates from Daudi cell line, human heart and pancreas tissue lysate(from left to right), using PACSIN1 Antibody (G23). AAA28726 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

WB (Western Blot) (Western blot analysis of lysates from human brain, mouse brain and rat brain tissue lysate (from left to right), using PACSIN1 Antibody (G23). AAA28726 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

WB (Western Blot) (Western blot analysis of lysates from Daudi cell line, human brain, human pancrease, human heart tissue (from left to right), using PACSIN1 Antibody (G23). AAA28726 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)

WB (Western Blot) (Western blot analysis of lysate from human heart tissue, using PACSIN1 Antibody (G23). AAA28726 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)

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PACSIN1, Polyclonal Antibody (Cat# AAA28726)

Full Name

PACSIN1 Antibody (N-term)

Gene Names

PACSIN1; SDPI

Reactivity

Human, mouse, rat (Predicted Reactivity: Rat, Chicken, Bovine, Xenopus)

Applications

Western Blot, Immunohistochemistry

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$275/0.08 mL $510/0.4 mL $975/2x0.4 mL $1,415/3x0.4 mL $1,825/4x0.4 mL $2,070/5x0.4 mL

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IHC (Immunohistchemistry) (Dilution: Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

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KiSS-1R, Polyclonal Antibody (Cat# AAA29058)

Full Name

KiSS-1R Polyclonal Antibody

Gene Names

KISS1R; HH8; GPR54; AXOR12; KISS-1R; HOT7T175

Reactivity

Human

Applications

Immunofluorescence

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

Application Data (Dilution: IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

Application Data (Dilution: IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 WB 1:500-2000, ELISA 1:10000-20000 IHC 1:50-300)

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SOCS-1, Polyclonal Antibody (Cat# AAA29121)

Full Name

SOCS-1 Polyclonal Antibody

Gene Names

SOCS1; JAB; CIS1; SSI1; TIP3; CISH1; SSI-1; SOCS-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts of various cell lines, using Aromatase at 1:3000 dilution.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded rat pancreas using Aromatase at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat ovary using Aromatase at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human placenta using Aromatase at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using Aromatase at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon using Aromatase at dilution of 1:100 (40x lens).)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using Aromatase at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

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Aromatase, Polyclonal Antibody (Cat# AAA30608)

Full Name

Aromatase Rabbit Polyclonal Antibody

Gene Names

CYP19A1; ARO; ARO1; CPV1; CYAR; CYP19; CYPXIX; P-450AROM

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

$360/0.05 mL $450/0.1 mL $855/2x0.1 mL $1,230/3x0.1 mL $1,595/4x0.1 mL $1,750/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of Jurkat cells with CD9 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti rabbit IgG (FITC) was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining CD9 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining CD9 in A549 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining CD9 in Lovo cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse large intestine tissue using anti-CD9 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-CD9 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-CD9 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of CD9 on HepG2 cell lysates using anti-CD9 antibody at 1/1000 dilution.)

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CD9, Polyclonal Antibody (Cat# AAA29968)

Full Name

CD9 Antibody

Gene Names

CD9; MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry

Purity

Peptide affinity purified

Pricing

$390/0.05 mL $510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,015/5x0.1 mL

WB (Western Blot) (Western BlotPositive WB detected in Recombinant proteinAll lanes: mpt64 antibody at 1.5ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 25 KDaObserved band size: 25 KDa)

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mpt64, Polyclonal Antibody (Cat# AAA26984)

Full Name

mpt64 Antibody

Reactivity

Mycobacterium tuberculosis

Applications

Western Blot

Purity

>95%, Protein G purified

Pricing

$265/0.05 mg $370/0.1 mg $690/2x0.1 mg $995/3x0.1 mg $1,285/4x0.1 mg $1,515/5x0.1 mg

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

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IL-33, Polyclonal Antibody (Cat# AAA29272)

Full Name

IL-33 Polyclonal Antibody

Gene Names

IL33; DVS27; IL1F11; NF-HEV; NFEHEV; C9orf26; RP11-575C20.2

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.