Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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IHC (Immunohistochemistry) (DAB staining on fromalin fixed paraffin-embedded Liver tissue.)

WB (Western Blot) (Western Blot;Sample: Mouse Liver lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse FGL1 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (Catalog:))

WB (Western Blot) (Western Blot;Sample: Recombinant FGL1, Mouse.)

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Fibrinogen Like Protein 1 (FGL1), Polyclonal Antibody (Cat# AAA20155)

Full Name

Polyclonal Antibody to Fibrinogen Like Protein 1 (FGL1)

Gene Names

Fgl1; Mfire1

Reactivity

Mouse, Rat

Applications

WB, IHC, ICC, IP

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

$245/0.1 mL $310/0.2 mL $475/0.5 mL $575/1 mL $1,515/5 mL $2,935/2x5 mL $4,275/3x5 mL $5,540/4x5 mL $6,750/5x5 mL

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IHC (Immunohistchemistry) (Anti- VCP Picoband antibody, AAA11662, IHC(F)IHC(F): Rat Brain Tissue)

IHC (Immunohistochemistry) (Anti- VCP Picoband antibody, AAA11662, IHC(F)IHC(F): Mouse Brain Tissue)

IHC (Immunohistochemistry) (Anti- VCP Picoband antibody, AAA11662, IHC(P)IHC(P): Human Glioma Tissue)

IHC (Immunohistochemistry) (Anti- VCP Picoband antibody, AAA11662, IHC(P)IHC(P): Rat Brain Tissue)

IHC (Immunohistochemistry) (Anti- VCP Picoband antibody, AAA11662, IHC(P)IHC(P): Mouse Brain Tissue)

WB (Western Blot) (Anti- VCP Picoband antibody, AAA11662, Western blottingAll lanes: Anti VCP (AAA11662) at 0.5ug/mlLane 1: Rat Brain Tissue Lysate at 50ugLane 2: Mouse Brain Tissue Lysate at 50ugLane 3: HELA Whole Cell Lysate at 40ugLane 4: 293T Whole Cell Lysate at 40ugPredicted bind size: 89KDObserved bind size: 89KD)

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VCP, Polyclonal Antibody (Cat# AAA11662)

Full Name

Anti-VCP Antibody

Gene Names

VCP; p97; TERA; ALS14; CMT2Y; IBMPFD; HEL-220; IBMPFD1; HEL-S-70

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Immunogen Affinity Purified

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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FCM (Flow Cytometry) (Figure 2. Flow Cytometry analysis of A549 cells using anti-WEE1 antibody (AAA19240).Overlay histogram showing A549 cells stained with AAA19240 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-WEE1 Antibody (AAA19240, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

WB (Western Blot) (Figure 1. Western blot analysis of WEE1 using anti-WEE1 antibody (AAA19240).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hela whole cell lysatesLane 2: human K562 whole cell lysatesLane 3: mouse lung tissue lysatesLane 4: mouse kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-WEE1 antigen affinity purified polyclonal antibody (Catalog # AAA19240) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog #) with Tanon 5200 system. A specific band was detected for WEE1 at approximately 72KD. The expected band size for WEE1 is at 72KD.)

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WEE1, Polyclonal Antibody (Cat# AAA19240)

Full Name

Anti-WEE1 Antibody

Gene Names

WEE1; WEE1A; WEE1hu

Reactivity

Human, Mouse

Applications

Western Blot, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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WB (Western Blot) (WB Suggested Anti-HCLS1 Antibody Titration: 1.25ug/mlPositive Control: Jurkat cell lysateHCLS1 is supported by BioGPS gene expression data to be expressed in Jurkat)

WB (Western Blot) (WB Suggested Anti-HCLS1 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:312500Positive Control: JurkatHCLS1 is supported by BioGPS gene expression data to be expressed in Jurkat)

WB (Western Blot) (Host: MouseTarget Name: HCLS1Sample Tissue: Mouse HeartAntibody Dilution: 1ug/ml)

IHC (Immunohistochemistry) (Rabbit Anti-HCLS1 AntibodyParaffin Embedded Tissue: Human StomachCellular Data: Epithelial cells of Fundic GlandAntibody Concentration: 4.0-8.0 ug/mlMagnification: 400X)

IHC (Immunohistochemistry) (Rabbit Anti-HCLS1 AntibodyParaffin Embedded Tissue: Human KidneyCellular Data: Epithelial cells of renal tubuleAntibody Concentration: 4.0-8.0 ug/mlMagnification: 400X)

IHC (Immunohistochemistry) (Rabbit Anti-HCLS1 AntibodyParaffin Embedded Tissue: Human HeartCellular Data: Myocardial cellsAntibody Concentration: 4.0-8.0 ug/mlMagnification: 400X)

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HCLS1, Polyclonal Antibody (Cat# AAA23399)

Full Name

HCLS1 antibody - N-terminal region

Gene Names

HCLS1; HS1; p75; CTTNL; lckBP1

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Protein A purified

Pricing

$475/0.1 mL $905/2x0.1 mL $1,310/3x0.1 mL $1,690/4x0.1 mL $1,890/5x0.1 mL

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 300ug extracts of Jurkat cells usingug FUS Polyclonal Antibody. Western blot was performed from the immunoprecipitate using FUS Polyclonal Antibody at a dilution of 1:1000.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse spleen using FUS Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using FUS Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast cancer using FUS Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using FUS Polyclonal Antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines using FUS Polyclonal Antibody at 1:1000 dilution.)

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FUS, Polyclonal Antibody (Cat# AAA22333)

Full Name

FUS Polyclonal Antibody

Gene Names

FUS; TLS; ALS6; ETM4; FUS1; POMP75; HNRNPP2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunoprecipitation

Purity

Affinity purification

Pricing

$325/0.06 mL $435/0.12 mL $655/0.2 mL $1,245/2x0.2 mL $1,810/3x0.2 mL $2,340/4x0.2 mL $2,855/5x0.2 mL

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IHC (Immunohistchemistry) (Immunohistochemistry Analysis: Representative lot data. (Fig. 1 and 2) Paraffin-embedded mouse and human brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:100 dilution. Reactivity was detected using the IHC-Select Detection Kit. Staining pattern appears as cytoplasmic. (Fig. 3 and 4) Paraffin-embedded mouse and mouse olfactory lobe and cerebellum brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a Chicken IgY Antibody 1:100 dilution of Cat. No. AB15894, anti-Tbr2. Reactivity was detected using the IHC-Select Detection Kit. Immunoreactivity seen here is mostly nuclear.)

IHC (Immunohistochemistry) (Paraffin-embedded mouse cerebellum was prepared using epitope retrieval in citrate buffer, pH 6.0. Staining was performed using 1:100 dilution. Immuno-reactivity is mostly nuclear.)

IHC (Immunohistochemistry) (Paraffin-embedded mouse olfactory lobe was prepared using epitope retrieval in citrate buffer, pH 6.0. Staining was performed using a 1:100 dilution. Reactivity is mostly nuclear.)

IS (Immunostaining) (Paraffin-embedded human brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:100 dilution. Staining pattern appears as cytoplasmic.)

IS (Immunostaining) (Paraffin-embedded mouse brain tissue was prepared using heat-induced epitope retrieval in citrate buffer, pH 6.0. Immunostaining was performed using a 1:100 dilution. Staining pattern appears as cytoplasmic.)

WB (Western Blot) (Western Blot Analysis: Representative lot data. E13-14 mouse brain lysate was resolved by electrophoresis, transferred to PVDF membranes and probed with (1:10,000) dilution. Proteins were visualized using a Rabbit Anti-Chicken conjugated to HRP and a chemi-luminescence detection system. Arrow indicates Tbr2 (~73 kD).)

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EOMES, Polyclonal Antibody (Cat# AAA26884)

Full Name

EOMES (Eomesodermin Homolog, T-box Brain Protein 2, TBR2, T-brain-2, TBR-2)

Gene Names

Eomes; Tbr2; TBR-2; C77258

Reactivity

Mouse, Human, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified by affinity chromatography.

Pricing

$1,355/0.1 mL $2,610/2x0.1 mL $3,795/3x0.1 mL $4,920/4x0.1 mL $5,805/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts of various cell lines, using TRAP1 at 1:3000 dilution.)

IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using TRAP1 at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using TRAP1 at dilution of 1:100. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Mouse kidney using TRAP1 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human colon carcinoma using TRAP1 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat brain using TRAP1 at dilution of 1:100 (40x lens).)

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TRAP1, Polyclonal Antibody (Cat# AAA30672)

Full Name

TRAP1 Rabbit Polyclonal Antibody

Gene Names

TRAP1; HSP75; HSP 75; HSP90L; TRAP-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

$360/0.05 mL $450/0.1 mL $855/2x0.1 mL $1,230/3x0.1 mL $1,595/4x0.1 mL $1,750/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using RIPK1/RIP Rabbit pAb.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human tonsil using RIPK1/RIP antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse spleen using RIPK1/RIP antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat lung using RIPK1/RIP antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using RIPK1/RIP antibody.)

WB (Western Blot) (Western blot analysis of extracts of C6 cells, using RIPK1/RIP antibody.)

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RIPK1, Polyclonal Antibody (Cat# AAA30689)

Full Name

RIPK1 Rabbit Polyclonal Antibody

Gene Names

RIPK1; RIP; RIP1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

$360/0.05 mL $450/0.1 mL $855/2x0.1 mL $1,230/3x0.1 mL $1,595/4x0.1 mL $1,750/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Thioredoxin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)

ICC (Immunocytochemistry) (ICC staining Thioredoxin in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Thioredoxin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Thioredoxin antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Thioredoxin antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Thioredoxin antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Thioredoxin on different cell lysates using anti-Thioredoxin antibody at 1/500 dilution. Positive control�� Lane1: Hela Lane2: HepG2 Lane3: A431 Lane4: PC-3M)

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Thioredoxin, Polyclonal Antibody (Cat# AAA29875)

Full Name

Thioredoxin Antibody

Gene Names

TXN; TRX; TRDX; TRX1

Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry

Purity

Peptide affinity purified

Pricing

$390/0.05 mL $510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,015/5x0.1 mL

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IHC (Immunohistchemistry) (Figure 6. IHC analysis of 14-3-3 zeta/delta using anti-14-3-3 zeta/delta antibody (AAA19144).14-3-3 zeta/delta was detected in paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-14-3-3 zeta/delta Antibody (AAA19144) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of 14-3-3 zeta/delta using anti-14-3-3 zeta/delta antibody (AAA19144).14-3-3 zeta/delta was detected in paraffin-embedded section of human mammary cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-14-3-3 zeta/delta Antibody (AAA19144) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of 14-3-3 zeta/delta using anti-14-3-3 zeta/delta antibody (AAA19144).14-3-3 zeta/delta was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-14-3-3 zeta/delta Antibody (AAA19144) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of 14-3-3 zeta/delta using anti-14-3-3 zeta/delta antibody (AAA19144).14-3-3 zeta/delta was detected in paraffin-embedded section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-14-3-3 zeta/delta Antibody (AAA19144) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Figure 2. Western blot analysis of 14-3-3 zeta/delta using anti-14-3-3 zeta/delta antibody (AAA19144).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat brain tissue lysates,Lane 2: rat spleen tissue lysates,Lane 3: rat lung tissue lysates,Lane 4: rat liver tissue lysates,Lane 5: mouse brain tissue lysates,Lane 6: mouse spleen tissue lysates,Lane 7: mouse lung tissue lysates,Lane 8: mouse liver tissue lysates,Lane 9: mouse kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-14-3-3 zeta/delta antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for 14-3-3 zeta/delta at approximately 28KD. The expected band size for 14-3-3 zeta/delta is at 28KD.)

WB (Western Blot) (Figure 1. Western blot analysis of 14-3-3 zeta/delta using anti-14-3-3 zeta/delta antibody (AAA19144).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human placenta tissue lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human A549 whole cell lysates,Lane 5: human PANC-1 whole cell lysates,Lane 6: human SK-OV-3 whole cell lysates,Lane 7: human 22RV1 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-14-3-3 zeta/delta antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for 14-3-3 zeta/delta at approximately 28KD. The expected band size for 14-3-3 zeta/delta is at 28KD.)

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14-3-3 zeta/delta, Polyclonal Antibody (Cat# AAA19144)

Full Name

Anti-14-3-3 zeta/delta Picoband antibody

Gene Names

YWHAZ; HEL4; YWHAD; KCIP-1; HEL-S-3; HEL-S-93; 14-3-3-zeta

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Applications

IHC, WB

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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WB (Western Blot) (WB Suggested Anti-ERCC8 antibody Titration: 1 ug/mLSample Type: Human liver)

WB (Western Blot) (WB Suggested Anti-ERCC8 Antibody Titration: 0.1-5.0ug/mlELISA Titer: 1:62500Positive Control: Jurkat cell lysate)

WB (Western Blot) (Host: RabbitTarget Name: ERCC8Sample Type: MCF7 Whole Cell lysatesAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: ERCC8Sample Type: Human HepG2Antibody Dilution: 1.0ug/mlERCC8 is supported by BioGPS gene expression data to be expressed in HepG2)

WB (Western Blot) (Host: RabbitTarget Name: ERCC8Sample Type: Human Fetal LungAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: ERCC8Sample Type: Human Fetal HeartAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: ERCC8Sample Type: Human Fetal BrainAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: RabbitTarget Name: ERCC8Sample Type: Human Adult PlacentaAntibody Dilution: 1.0ug/ml)

IHC (Immunohistochemistry) (HumanMuscle)

IHC (Immunohistochemistry) (Human Liver)

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ERCC8, Polyclonal Antibody (Cat# AAA23386)

Full Name

ERCC8 antibody - C-terminal region

Gene Names

ERCC8; CSA; CKN1; UVSS2

Reactivity

Dog, Horse, Human, Pig, Rabbit, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purified

Pricing

$530/0.1 mL $1,005/2x0.1 mL $1,455/3x0.1 mL $1,885/4x0.1 mL $2,140/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using IKBKB antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse brain using IKBKB antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using IKBKB antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using IKBKB antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using IKBKB antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using IKBKB antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using IKBKB antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

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IKBKB, Polyclonal Antibody (Cat# AAA10671)

Full Name

IKBKB Polyclonal Antibody

Gene Names

IKBKB; IKK2; IKKB; IMD15; NFKBIKB; IKK-beta

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of K562 cells using anti-RCC1 antibody (AAA19263).Overlay histogram showing K562 cells stained with AAA19263 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RCC1 Antibody (AAA19263,1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 9. IF analysis of RCC1 using anti-RCC1 antibody (AAA19263).RCC1 was detected in immunocytochemical section of Hela cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 4μg/mL rabbit anti-RCC1 Antibody (AAA19263) overnight at 4 degree C. DyLight®594 Conjugated Goat Anti-Rabbit IgG (BA1142) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IF (Immunofluorescence) (Figure 8. IF analysis of RCC1 using anti- RCC1 antibody (AAA19263).RCC1 was detected in paraffin-embedded section of human intestine cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 4μg/mL rabbit anti- RCC1 Antibody (AAA19263) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of RCC1 using anti- RCC1 antibody (AAA19263).RCC1 was detected in frozen section of human placenta tissue. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti- RCC1 Antibody (AAA19263) overnight at 4 degree C. DyLight®488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC)(Catalog # with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of RCC1 using anti-RCC1 antibody (AAA19263).RCC1 was detected in paraffin-embedded section of rat lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RCC1 Antibody (AAA19263) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of RCC1 using anti-RCC1 antibody (AAA19263).RCC1 was detected in paraffin-embedded section of mouse lung tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RCC1 Antibody (AAA19263) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of RCC1 using anti-RCC1 antibody (AAA19263).RCC1 was detected in paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RCC1 Antibody (AAA19263) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of RCC1 using anti-RCC1 antibody (AAA19263).RCC1 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RCC1 Antibody (AAA19263) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of RCC1 using anti-RCC1 antibody (AAA19263).RCC1 was detected in paraffin-embedded section of human rectal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-RCC1 Antibody (AAA19263) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of RCC1 using anti-RCC1 antibody (AAA19263).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HEK293 whole cell lysatesLane 2: human A431 whole cell lysatesLane 3: human HepG2 whole cell lysatesLane 4: human Raji whole cell lysatesLane 5: human THP-1 whole cell lysatesLane 6: rat brain tissue lysatesLane 7: mouse Neuro-2a whole cell lysatesLane 8: mouse NIH/3T3 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-RCC1 antigen affinity purified polyclonal antibody (Catalog # AAA19263) at 0. 25 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for RCC1 at approximately 45KD. The expected band size for RCC1 is at 45KD.)

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RCC1, Polyclonal Antibody (Cat# AAA19263)

Full Name

Anti-RCC1 Antibody

Gene Names

RCC1; CHC1; RCC1-I; SNHG3-RCC1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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WB (Western Blot) (Western Blot;Sample:Lane 1: Rat Plasma;Lane 2: Rat Liver lysate;Lane 3: Rat Serum;Lane 4: Mouse SerumPrimary Ab: 0.04ug/ml Rabbit Anti-Rat C3 AntibodySecond Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

WB (Western Blot) (Western Blot;Sample: Recombinant C3, Rat.)

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Complement Component 3 (C3), Polyclonal Antibody (Cat# AAA20147)

Full Name

Polyclonal Antibody to Complement Component 3 (C3)

Reactivity

Mouse, Rat

Applications

WB, IHC, ICC, IP

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

$240/0.1 mL $305/0.2 mL $460/0.5 mL $555/1 mL $1,445/5 mL $2,790/2x5 mL $4,065/3x5 mL $5,270/4x5 mL $6,430/5x5 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human esophageal cancer using TET3 Rabbit pAb.)

IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using TET3 Rabbit pAb)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using TET3 antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using TET3 antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 using TET3 antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using TET3 antibody.)

WB (Western Blot) (Western blot analysis of extracts of rat thymus, using TET3 antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using TET3 antibody.)

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TET3, Polyclonal Antibody (Cat# AAA29847)

Full Name

TET3 Polyclonal Antibody

Gene Names

TET3; hCG_40738

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunofluorescence, Immunohistochemistry

Purity

Affinity purification

Pricing

$360/0.05 mL $450/0.1 mL $855/2x0.1 mL $1,230/3x0.1 mL $1,595/4x0.1 mL $1,750/5x0.1 mL

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IHC (Immunohistchemistry) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

IHC (Immunohistochemistry) (Dilution: IHC-p 1:50-200, ELISA 1:10000-20000)

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IL-17D, Polyclonal Antibody (Cat# AAA29384)

Full Name

IL-17D Polyclonal Antibody

Gene Names

IL17D; IL27; IL-27; IL-17D

Reactivity

Human

Applications

Immunohistochemistry

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

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WB (Western Blot) (Western blot analysis of CARD6 Antibody (Center) in MDA-MB231 cell line lysates (35ug/lane). CARD6 (arrow) was detected using the purified Pab.)

WB (Western Blot) (CARD6 Antibody (Center) western blot analysis in WiDr cell line lysates (35ug/lane).This demonstrates the CARD6 antibody detected the CARD6 protein (arrow).)

WB (Western Blot) (Western blot analysis of lysates from A549, HepG2 cell line (from left to right), using CARD6 Antibody (Center). AAA28742 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)

WB (Western Blot) (Western blot analysis of lysate from WiDr cell line, using CARD6 Antibody (Center). AAA28742 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:5000 dilution was used as the secondary antibody. Lysate at 20ug.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using CARD6 Antibody (Center). AAA28742 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded H. colon section using CARD6 Antibody (Center). AAA28742 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded H. skeletal muscle section using CARD6 Antibody (Center). AAA28742 was diluted at 1:25 dilution. A undiluted biotinylated goat polyvalent antibody was used as the secondary, followed by DAB staining.)

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CARD6, Polyclonal Antibody (Cat# AAA28742)

Full Name

CARD6 Antibody (Center)

Gene Names

CARD6; CINCIN1

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$275/0.08 mL $510/0.4 mL $975/2x0.4 mL $1,415/3x0.4 mL $1,825/4x0.4 mL $2,070/5x0.4 mL

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

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CD1D, Polyclonal Antibody (Cat# AAA29189)

Full Name

CD1D Polyclonal Antibody

Gene Names

CD1D; R3; CD1A

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

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IHC (Immunohistochemistry) (At 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistchemistry) (At 1/100 staining Mouse colorectal tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat stomach tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (Staining Rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (At 1/100 staining Rat muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

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YB1, Polyclonal Antibody (Cat# AAA31294)

Full Name

Phospho-YB1 (Ser102) Antibody

Gene Names

YBX1; YB1; BP-8; CSDB; DBPB; YB-1; CSDA2; NSEP1; NSEP-1; MDR-NF1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Peptide ELISA

Purity

The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.

Pricing

$415/0.1 mL $485/0.2 mL $925/2x0.2 mL $1,340/3x0.2 mL $1,725/4x0.2 mL $1,900/5x0.2 mL

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IHC (Immunohistchemistry) (AAA31091 at 1/200 staining human breast cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of extracts of various tissue sample, using VEGFR2 antibody.)

IHC (Immunohistochemistry) (AAA31091 at 1/200 staining human breast cancer tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IHC (Immunohistochemistry) (AAA31091 at 1/50 staining human breast cancer tissue by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA31091 staining K562 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of VEGFR2 expression in SK-OV3 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

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VEGFR2, Polyclonal Antibody (Cat# AAA31091)

Full Name

VEGFR2 Antibody

Gene Names

KDR; FLK1; CD309; VEGFR; VEGFR2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$360/0.1 mL $415/0.2 mL $785/2x0.2 mL $1,130/3x0.2 mL $1,455/4x0.2 mL $1,555/5x0.2 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse spleen using IL2 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse lung using IL2 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung using IL2 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human tonsil using IL2 at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of Raw264.7 cells, using IL2 at 1:1000 dilution. Raw264.7 cells were treated by LPS for 6 hours and Brefeldin A for 3 hours of stimulation.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using IL2 at 1:1000 dilution.)

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IL2, Polyclonal Antibody (Cat# AAA30640)

Full Name

IL2 Rabbit Polyclonal Antibody

Gene Names

IL2; IL-2; TCGF; lymphokine

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Affinity purification

Pricing

$360/0.05 mL $450/0.1 mL $855/2x0.1 mL $1,230/3x0.1 mL $1,595/4x0.1 mL $1,750/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using Fibroblast activation protein-?? (FAP) Rabbit pAb.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using Fibroblast activation protein-?? (FAP) Rabbit pAb.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse colon using Fibroblast activation protein-?? (FAP) Rabbit pAb.)

IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using Fibroblast activation protein-?? (FAP) Rabbit pAb.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human tonsil using Fibroblast activation protein-?? (FAP) Rabbit pAb.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast cancer using Fibroblast activation protein-?? (FAP) Rabbit pAb.)

WB (Western Blot) (Western blot analysis of extracts of NIH/3T3 cells, using Fibroblast activation protein-?? (FAP) antibody.)

WB (Western Blot) (Western blot analysis of extracts of Rat skeletal muscle, using Fibroblast activation protein-?? (FAP) antibody.)

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FAP, Polyclonal Antibody (Cat# AAA30680)

Full Name

FAP Rabbit Polyclonal Antibody

Gene Names

FAP; FAPA; DPPIV

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

$360/0.05 mL $450/0.1 mL $855/2x0.1 mL $1,230/3x0.1 mL $1,595/4x0.1 mL $1,750/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

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Bad, Polyclonal Antibody (Cat# AAA28968)

Full Name

Bad Polyclonal Antibody

Gene Names

BAD; BBC2; BCL2L8

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemical analysis of (AAA28653) on paraffin-embedded Human breastcarcinoma tissue. Tissue was fixed withformaldehyde at room temperature. Heatinduced epitope retrieval was performed byEDTA buffer (pH9. 0). Samples wereincubated with primary antibody(1:100) for 1hour at room temperature. Undiluted CRFAnti-Polyvalent HRP Polymer antibody wasused as the secondary antibody.)

WB (Western Blot) (Western blot analysis of lysates from A2780 cell line, untreated or treated with Alkaline phosphatase, 1h, using 459085101 (AAA28653) or Beta-actin (lower).)

WB (Western Blot) (Western blot analysis of lysates from A2780 cell line, untreated or treated with Alkaline phosphatase, 1h, using 459088101 (AAA28653) (upper) or Beta-actin (lower).)

WB (Western Blot) (Western blot analysis of lysates from 293 cell line, untreated or treated with Alkaline phosphatase, 1h, using 459088102(AAA28653) (upper) or Beta-actin (lower).)

IHC (Immunohistochemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot) (The anti-phospho CDC25A S124 Pab is used in Western blot to detect CDC25A in mouse liver tissue lysate)

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Phospho-CDC25A (S124), Polyclonal Antibody (Cat# AAA28653)

Full Name

Phospho-CDC25A (S124) Antibody

Gene Names

CDC25A; CDC25A2

Reactivity

Human (Predicted Reactivity: Rat)

Applications

WB, EIA, IHC

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$275/0.08 mL $510/0.4 mL $975/2x0.4 mL $1,415/3x0.4 mL $1,825/4x0.4 mL $2,070/5x0.4 mL

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IF (Immunofluorescence) (Figure 7 Immunofluorescence Validation of TMPRSS2 in Rat BrainImmunofluorescent analysis of 4% paraformaldehyde-fixed rat brain labeling TMPRSS2 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)

IF (Immunofluorescence) (Figure 6 Immunofluorescence Validation of TMPRSS2 in Mouse BrainImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse brain labeling TMPRSS2 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)

IF (Immunofluorescence) (Figure 5 Immunofluorescence Validation of TMPRSS2 in Human LungImmunofluorescent analysis of 4% paraformaldehyde-fixed human lung labeling TMPRSS2 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)

IF (Immunofluorescence) (Figure 4 Immunofluorescence Validation of TMPRSS2 in A549 CellsImmunofluorescent analysis of 4% paraformaldehyde-fixed A549 cells labeling TMPRSS2 at 20ug/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (green) and DAPI staining (blue).)

WB (Western Blot) (Figure 3 Western Blot Validation in Rat TissuesLoading: 15 ug of lysates per lane. Antibodies: TMPRSS2, 2ug/mL, 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)

WB (Western Blot) (Figure 2 Western Blot Validation in Mouse Tissues Loading: 15 ug of lysates per lane.Antibodies: TMPRSS2 9571, 2ug/mL, 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10,000 dilution.)

WB (Western Blot) (Figure 1 WB Validation in Human Cell Lines Loading: 15 ug of lysate Antibodies: TMPRSS2, 2ug/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat Anti-Rabbit IgG HRP conjugate at 1:10,000 dilution.)

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TMPRSS2, Polyclonal Antibody (Cat# AAA11038)

Full Name

TMPRSS2 (IN) Antibody

Gene Names

TMPRSS2; PP9284; PRSS10

Reactivity

Human, Mouse, Rat
Predicted species reactivity based on immunogen sequence: Horse (100%); Rabbit (100%); Monkey (100%); Sheep (100%); Gorilla (100%); Cat (100%).

Applications

Immunofluorescence, Western Blot

Purity

TMPRSS2 Antibody is affinity chromatography purified via peptide column.

Pricing

$570/0.1 mg $1,080/2x0.1 mg $1,560/3x0.1 mg $2,015/4x0.1 mg $2,295/5x0.1 mg

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ICC (Immunocytochemistry) (Anti-MCM3 antibody, AAA11518, ICCICC: HELA Cell)

IHC (Immunohistochemistry) (Anti-MCM3 antibody, AAA11518, IHC(P)IHC(P): Mouse Intestine Tissue)

IHC (Immunohistochemistry) (Anti-MCM3 antibody, AAA11518, IHC(P)IHC(P): Rat Intestine Tissue)

IHC (Immunohistochemistry) (Anti-MCM3 antibody, AAA11518, IHC(P)IHC(P): Rat Intestine Tissue)

IHC (Immunohistochemistry) (Anti-MCM3 antibody, AAA11518, IHC(P)IHC(P): Human Lung Cancer Tissue)

WB (Western Blot) (Anti-MCM3 antibody, AAA11518, Western blottingAll lanes: Anti MCM3 (AAA11518) at 0.5ug/mlLane 1: NIH3T3 Whole Cell Lysate at 40ugLane 2: HEPA Whole Cell Lysate at 40ugPredicted bind size: 91KDObserved bind size: 91KD)

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MCM3, Polyclonal Antibody (Cat# AAA11518)

Full Name

Anti-MCM3 antibody

Gene Names

MCM3; HCC5; P1.h; RLFB; P1-MCM3

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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IF (Immunofluorescence) (Immunofluorescence analysis of MCF-7 cells using PSAP antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse spleen using PSAP Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse brain using PSAP Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver using PSAP Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using PSAP Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse spleen using PSAP Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver using PSAP Antibody at dilution of 1:200 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using PSAP antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

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PSAP, Polyclonal Antibody (Cat# AAA10696)

Full Name

PSAP Polyclonal Antibody

Gene Names

PSAP; GLBA; SAP1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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IHC (Immunohistchemistry) (Anti-DUSP23 antibody IHC of human Lung, Non-Small Cell Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemistry) (Anti-DUSP23 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemistry) (Anti-DUSP23 antibody IHC of human brain, substantia nigra. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemistry) (Anti-DUSP23 antibody IHC of human uterus, endometrium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10-20 ug/ml.)

IHC (Immunohistochemistry) (Anti-DUSP23 antibody IHC of human brain, hippocampus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemistry) (Anti-DUSP23 antibody IHC of human colon, epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody dilution 10-20 ug/ml.)

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DUSP23, Polyclonal Antibody (Cat# AAA12325)

Full Name

Rabbit Polyclonal to Human DUSP23

Gene Names

DUSP23; VHZ; MOSP; LDP-3; DUSP25

Reactivity

Gibbon, Gorilla, Human
Predicted Reactivity: Monkey, Horse, Rabbit (at least 90% immunogen sequence identity)

Applications

Immunohistochemistry

Purity

Immunoaffinity Purified

Pricing

$690/0.05 mg $1,325/2x0.05 mg $1,915/3x0.05 mg $2,475/4x0.05 mg $2,800/5x0.05 mg

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IHC (Immunohistchemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot) (Western blot analysis of EPHA4 (arrow) using EphA4 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the EPHA4 gene (Lane 2) (Origene Technologies).)

WB (Western Blot) (Western blot analysis of anti-EphA4 Pab in NCI-H460 cell lysate. EphA4 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

WB (Western Blot) (Western blot analysis of lysates from Hela, NCI-H460, mouse NIH/3T3 cell line and human ovary tissue lysate (from left to right), using EPHA4 Antibody (R890). AAA28736 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35ug per lane.)

WB (Western Blot) (Western blot analysis of lysates from Hela, NCI-H460, HUVEC cell line (from left to right), using EPHA4 Antibody (R890). AAA28736 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)

WB (Western Blot) (Western blot analysis of lysates from Hela, HUVEC cell line (from left to right), using EPHA4 Antibody (R890). AAA28736 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)

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EphA4, Polyclonal Antibody (Cat# AAA28736)

Full Name

EphA4 Antibody (C-term)

Gene Names

EPHA4; SEK; HEK8; TYRO1

Reactivity

Human, mouse

Applications

Western Blot, Immunohistochemistry

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$275/0.05 mL $510/0.2 mL $975/2x0.2 mL $1,415/3x0.2 mL $1,825/4x0.2 mL $2,070/5x0.2 mL

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ICC (Immunocytochemistry) (ICC staining NSUN4 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining NSUN4 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining NSUN4 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-NSUN4 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-NSUN4 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-NSUN4 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of NSUN4 on different cell lysates using anti-NSUN4 antibody at 1/1, 000 dilution. Positive control�� Lane1: MCF-7 Lane2: 293)

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NSUN4, Polyclonal Antibody (Cat# AAA29886)

Full Name

NSUN4 Antibody

Gene Names

NSUN4; SHTAP

Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,015/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/10000. Not yet tested in other applications.)

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CD26, Polyclonal Antibody (Cat# AAA29320)

Full Name

CD26 Polyclonal Antibody

Gene Names

DPP4; CD26; ADABP; ADCP2; DPPIV; TP103

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

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Tubulin alpha, Polyclonal Antibody (Cat# AAA29254)

Full Name

Tubulin alpha Polyclonal Antibody

Gene Names

TUBA1A; LIS3; TUBA3; B-ALPHA-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

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IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1:100-1:300. ELISA: 1/20000. Not yet tested in other applications.)

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OAS2, Polyclonal Antibody (Cat# AAA29290)

Full Name

OAS2 Polyclonal Antibody

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

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ICC (Immunocytochemistry) (Figure 6. IHC analysis of LYRIC using anti-LYRIC antibody (AAA11650).LYRIC was detected in immunocytochemical section of SMMC-7721 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-LYRIC Antibody (AAA11650) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

ICC (Immunocytochemistry) (Figure 5. IHC analysis of LYRIC using anti-LYRIC antibody (AAA11650).LYRIC was detected in immunocytochemical section of Hela cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-LYRIC Antibody (AAA11650) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of LYRIC using anti-LYRIC antibody (AAA11650).LYRIC was detected in immunocytochemical section of A549 cell. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-LYRIC Antibody (AAA11650) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of LYRIC using anti-LYRIC antibody (AAA11650).LYRIC was detected in paraffin-embedded section of Human Mammary Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-LYRIC Antibody (AAA11650) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of LYRIC using anti-LYRIC antibody (AAA11650).LYRIC was detected in paraffin-embedded section of Rat Skeletal Muscle Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-LYRIC Antibody (AAA11650) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of LYRIC using anti-LYRIC antibody (AAA11650).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Rat Skeletal Muscle Tissue Lysate,Lane 2: Mouse Skeletal Muscle Tissue Lysate,Lane 3: Rat Cardiac Muscle Tissue Tissue Lysate,Lane 4: Mouse Cardiac Muscle Tissue Lysate,Lane 5: MCF-7 Whole Cell Lysate,Lane 6: U87 Whole Cell Lysate,Lane 7: 22RV1 Whole Cell Lysate,Lane 8: PC-12 Whole Cell Lysate.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-LYRIC antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for LYRIC at approximately 100KD. The expected band size for LYRIC is at 64KD.)

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LYRIC, Polyclonal Antibody (Cat# AAA11650)

Full Name

Anti-LYRIC Antibody

Gene Names

MTDH; 3D3; AEG1; AEG-1; LYRIC; LYRIC/3D3

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Immunogen Affinity Purified

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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IF (Immunofluorescence) (Immunofluorescence analysis of MCF-7 cells using STK11 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human uterine cancer using STK11 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using STK11 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using STK11 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human stomach using STK11 antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human esophageal cancer using STK11 antibody at dilution of 1:200 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using STK11 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)

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STK11, Polyclonal Antibody (Cat# AAA10694)

Full Name

STK11 Polyclonal Antibody

Gene Names

STK11; PJS; LKB1; hLKB1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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Transfection Control (Anti-GPR4 antibody immunocytochemistry (ICC) staining of untransfected HEK293 human embryonic kidney cells.)

Transfection (Anti-GPR4 antibody immunocytochemistry (ICC) staining of HEK293 human embryonic kidney cells transfected with GPR4.)

IHC (Immunohistochemistry) (Anti-GPR4 antibody IHC of human Lung, Adenocarcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemistry) (Anti-GPR4 antibody IHC of human Skin, Melanoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemistry) (Anti-GPR4 antibody IHC of human lung, respiratory epithelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemistry) (Anti-GPR4 antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

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GPR4, Polyclonal Antibody (Cat# AAA12324)

Full Name

Rabbit Polyclonal to Human GPR4

Reactivity

Gibbon, Dog, Gorilla, Human, Monkey
Predicted Reactivity: Mouse, Rat, Bat, Bovine, Pig (at least 90% immunogen sequence identity)

Applications

Immunohistochemistry, Immunocytochemistry

Purity

Immunoaffinity Purified

Pricing

$670/0.05 mg $1,275/2x0.05 mg $1,860/3x0.05 mg $2,405/4x0.05 mg $2,695/5x0.05 mg

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IHC (Immunohistchemistry) (Figure 6. IHC analysis of Sigma1-Receptor/SIGMAR1 using anti-Sigma1-Receptor/SIGMAR1 antibody (AAA19462).Sigma1-Receptor/SIGMAR1 was detected in a paraffin-embedded section of human liver cancer tissue was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Sigma1-Receptor/SIGMAR1 Antibody (AAA19462) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of Sigma1-Receptor/SIGMAR1 using anti-Sigma1-Receptor/SIGMAR1 antibody (AAA19462).Sigma1-Receptor/SIGMAR1 was detected in a paraffin-embedded section of human stomach cancer tissue was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Sigma1-Receptor/SIGMAR1 Antibody (AAA19462) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of Sigma1-Receptor/SIGMAR1 using anti-Sigma1-Receptor/SIGMAR1 antibody (AAA19462).Sigma1-Receptor/SIGMAR1 was detected in a paraffin-embedded section of human adenocarcinoma of the right colon tissue was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Sigma1-Receptor/SIGMAR1 Antibody (AAA19462) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of Sigma1-Receptor/SIGMAR1 using anti-Sigma1-Receptor/SIGMAR1 antibody (AAA19462).Sigma1-Receptor/SIGMAR1 was detected in a paraffin-embedded section of mouse colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Sigma1-Receptor/SIGMAR1 Antibody (AAA19462) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of Sigma1-Receptor/SIGMAR1 using anti-Sigma1-Receptor/SIGMAR1 antibody (AAA19462).Sigma1-Receptor/SIGMAR1 was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Sigma1-Receptor/SIGMAR1 Antibody (AAA19462) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of Sigma1-Receptor/SIGMAR1 using anti-Sigma1-Receptor/SIGMAR1 antibody (AAA19462).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human Caco-2 whole cell lysates,Lane 3: human 293T whole cell lysates,Lane 4: human HepG2 whole cell lysates,Lane 5: human A549 whole cell lysates,Lane 6: human COLO-320 whole cell lysates,Lane 7: human U-87MG whole cell lysates,Lane 8: monkey COS-7 whole cell lysates,Lane 9: mouse liver tissue lysates,Lane 10: mouse C2C12 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Sigma1-Receptor/SIGMAR1 antigen affinity purified polyclonal antibody (#AAA19462) at 0.25 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Sigma1-Receptor/SIGMAR1 at approximately 25 kDa. The expected band size for Sigma1-Receptor/SIGMAR1 is at 25 kDa.)

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Sigma1-receptor/SIGMAR1, Polyclonal Antibody (Cat# AAA19462)

Full Name

Anti-Sigma1-receptor/SIGMAR1 Antibody Picoband

Gene Names

SIGMAR1; SRBP; ALS16; OPRS1; SR-BP1

Reactivity

Human, Monkey, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human esophagus Tissue.)

IHC (Immunohistchemistry) (DAB staining on IHC-P; Samples: Human Rectum Cancer Tissue)

IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Kidney Tissue)

IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Rectum Tissue))

IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Rectum Tissue.)

WB (Western Blot) (Western Blot: Sample: Recombinant ACTa2, Human.)

WB (Western Blot) (Western Blot: Sample: Lane1: Mouse Brain Tissue; Lane2: Mouse Heart Tissue; Lane3: Mouse Skeletal Muscle Tissue.)

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Actin Alpha 2 (ACTa2), Polyclonal Antibody (Cat# AAA21048)

Full Name

Anti-Actin Alpha 2, Smooth Muscle (ACTa2) polyclonal Antibody

Gene Names

ACTA2; ACTSA

Reactivity

Human, Mouse, Rat, Guinea pig, Rabbit, Simian, Dog, Pig, Bovine, Horse, Chicken, Zebrafish

Applications

Western Blot

Purity

Antigen-specific affinity chromatography

Pricing

$245/0.1 mL $310/0.2 mL $475/0.5 mL $575/1 mL $1,505/5 mL $2,915/2x5 mL $4,245/3x5 mL $5,500/4x5 mL $6,715/5x5 mL

WB (Western Blot) (Western Blot analysis of A549 cells with EIF2AK1 Polyclonal Antibody.)

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HRI, Polyclonal Antibody (Cat# AAA22059)

Full Name

EIF2AK1 Polyclonal Antibody

Reactivity

Human, Mouse, Monkey

Applications

Western Blot, Immunohistochemistry

Purity

Affinity purification

Pricing

$205/0.02 mL $255/0.06 mL $370/0.12 mL $545/0.2 mL $1,030/2x0.2 mL $1,485/3x0.2 mL $1,920/4x0.2 mL $2,165/5x0.2 mL

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IHC (Immunohistchemistry) (Immunochemical staining NRP1 in human liver with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry) (Immunochemical staining NRP1 in human brain with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry) (Immunochemical staining NRP1 in rat liver with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry) (Immunochemical staining NRP1 in rat brain with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry) (Immunochemical staining NRP1 in mouse liver with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

IHC (Immunohistochemistry) (Immunochemical staining NRP1 in mouse brain with rabbit polyclonal antibody at 1:300 dilution, formalin-fixed paraffin embedded sections.)

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Neuropilin-1, Polyclonal Antibody (Cat# AAA27735)

Full Name

Anti-Neuropilin-1 Antibody, Rabbit Polyclonal

Gene Names

Nrp1; Nrp; NP-1; Npn1; NPN-1; C530029I03

Reactivity

Mouse, Rat, Human

Applications

Immunohistochemistry

Purity

Protein A & Antigen Affinity

Pricing

$405/0.1 mL $760/2x0.1 mL $1,105/3x0.1 mL $1,420/4x0.1 mL $1,520/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of L929 cells using MT-ND1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using MT-ND1 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using MT-ND1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human esophageal using MT-ND1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat ovary using MT-ND1 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using MT-ND1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)

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MT-ND1, Polyclonal Antibody (Cat# AAA28360)

Full Name

MT-ND1 Rabbit pAb

Gene Names

MT-ND1; MTND1; ND1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using MyD88 Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa using MyD88 Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using MyD88 Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells using MyD88 Rabbit pAb at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of Rat spleen, using MyD88 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.)

WB (Western Blot) (Western blot analysis of extracts of Mouse spleen, using MyD88 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)

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MyD88, Polyclonal Antibody (Cat# AAA28339)

Full Name

MyD88 Rabbit pAb

Gene Names

MYD88; MYD88D

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunofluorescence

Purity

Affinity purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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WB (Western Blot) (Western blot analysis of 293T mouse-kidney lysate, antibody was diluted at 2000. Secondary antibody was diluted at 1:20000)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human-kidney-cancer, antibody was diluted at 1:200)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human-kidney-cancer, antibody was diluted at 1:200)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human-stomach-cancer, antibody was diluted at 1:200)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded Human skin. 1, Antibody was diluted at 1:200(4 degree overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human skin. 1, Antibody was diluted at 1:200(4 degree overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human skin. 1, Antibody was diluted at 1:200(4 degree overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human gastric cancer. 1, Antibody was diluted at 1:100(4 degree overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human gastric cancer. 1, Antibody was diluted at 1:100(4 degree overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human gastric cancer. 1, Antibody was diluted at 1:100(4 degree overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

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EGF, Polyclonal Antibody (Cat# AAA30755)

Full Name

EGF Rabbit Polyclonal Antibody

Gene Names

EGF; URG; HOMG4

Reactivity

Human, Rat, Mouse

Applications

Western Blot, Immunohistochemistry, Immunohistochemistry

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$335/0.05 mL $415/0.1 mL $785/2x0.1 mL $1,130/3x0.1 mL $1,455/4x0.1 mL $1,550/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using ZNF703 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast cancer using ZNF703 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using ZNF703 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat pancreas using ZNF703 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using ZNF703 at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of MCF7 cells, using ZNF703 at 1:1000 dilution.)

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ZNF703, Polyclonal Antibody (Cat# AAA30623)

Full Name

ZNF703 Rabbit Polyclonal Antibody

Gene Names

ZNF703; NLZ1; ZPO1; ZEPPO1; ZNF503L

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

$360/0.05 mL $450/0.1 mL $855/2x0.1 mL $1,230/3x0.1 mL $1,595/4x0.1 mL $1,750/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human thyroid cancer using CPSF6 Rabbit pAb.)

IF (Immunofluorescence) (Confocal immunofluorescence analysis of U2OS cells using CPSF6 Polyclonal Antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat testis using CPSF6 Rabbit pAb.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse lung using CPSF6 Rabbit pAb.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human esophageal cancer using CPSF6 Rabbit pAb.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using CPSF6 antibody.)

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CPSF6, Polyclonal Antibody (Cat# AAA30676)

Full Name

CPSF6 Rabbit Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

$360/0.05 mL $450/0.1 mL $855/2x0.1 mL $1,230/3x0.1 mL $1,595/4x0.1 mL $1,750/5x0.1 mL

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IF (Immunofluorescence) (Confocal immunofluorescence analysis of U2OS cells using KU70 Polyclonal at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells using KU70. Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using KU70 at 1:1000 dilution.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human uterus using KU70 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using KU70 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using KU70 at dilution of 1:100 (40x lens).)

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KU70, Polyclonal Antibody (Cat# AAA30688)

Full Name

KU70 Rabbit Polyclonal Antibody

Gene Names

XRCC6; ML8; KU70; TLAA; CTC75; CTCBF; G22P1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

$360/0.05 mL $450/0.1 mL $855/2x0.1 mL $1,230/3x0.1 mL $1,595/4x0.1 mL $1,750/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: WB 1:500-2000, ELISA 1:10000-20000)

WB (Western Blot) (Dilution: WB 1:500-2000, ELISA 1:10000-20000)

IHC (Immunohistochemistry) (Dilution: WB 1:500-2000, ELISA 1:10000-20000)

IHC (Immunohistochemistry-Paraffin) (Dilution: WB 1:500-2000, ELISA 1:10000-20000)

IHC (Immunohistochemistry-Paraffin) (Dilution: WB 1:500-2000, ELISA 1:10000-20000)

IHC (Immunohistochemistry-Paraffin) (Dilution: WB 1:500-2000, ELISA 1:10000-20000)

WB (Western Blot) (Dilution: WB 1:500-2000, ELISA 1:10000-20000)

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CYFIP2, Polyclonal Antibody (Cat# AAA29330)

Full Name

CYFIP2 Polyclonal Antibody

Gene Names

CYFIP2; PIR121

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts of various cell lines, using NUMB antibody.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using NUMB Rabbit pAb.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver using NUMB Rabbit pAb.)

IF (Immunofluorescence) (Immunofluorescence analysis of L929 cells using NUMB antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using NUMB Rabbit pAb.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using NUMB Rabbit pAb.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using NUMB antibody.)

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NUMB, Polyclonal Antibody (Cat# AAA30713)

Full Name

NUMB Rabbit Polyclonal Antibody

Gene Names

NUMB; S171; C14orf41; c14_5527

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, IF

Purity

Affinity purification

Pricing

$360/0.05 mL $450/0.1 mL $855/2x0.1 mL $1,230/3x0.1 mL $1,595/4x0.1 mL $1,750/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using DCK antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human uterine cancer using DCK antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using DCK antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human vermiform appendix using DCK antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using DCK antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using DCK Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)

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DCK, Polyclonal Antibody (Cat# AAA10732)

Full Name

DCK Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

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WB (Western Blot) (Figure 1. Western blot analysis of NHEJ1 using anti-NHEJ1 antibody.)

IHC (Immunohistchemistry)

IHC (Immunohistochemistry)

IF (Immunofluorescence)

IF (Immunofluorescence)

FCM (Flow Cytometry)

FCM (Flow Cytometry)

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XLF/NHEJ1, Polyclonal Antibody (Cat# AAA19203)

Full Name

Anti-XLF/NHEJ1 Antibody

Gene Names

NHEJ1; XLF

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.