Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of A549 cells using anti-SHKBP1 antibody (AAA19991).Overlay histogram showing A549 cells stained with AAA19991 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SHKBP1 Antibody (AAA19991, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistchemistry) (Figure 9. IHC analysis of SHKBP1 using anti-SHKBP1 antibody (AAA19991).SHKBP1 was detected in a paraffin-embedded section of human lung adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SHKBP1 Antibody (AAA19991) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SHKBP1 Antibody (AAA19991) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SHKBP1 Antibody (AAA19991) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SHKBP1 Antibody (AAA19991) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SHKBP1 Antibody (AAA19991) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SHKBP1 Antibody (AAA19991) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SHKBP1 Antibody (AAA19991) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SHKBP1 Antibody (AAA19991) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HEL whole cell lysates,Lane 3: human U251 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHKBP1 antigen affinity purified polyclonal antibody (#AAA19991) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SHKBP1 at approximately 76 kDa. The expected band size for SHKBP1 is at 76 kDa.)

Compare (5 max)

SHKBP1, Polyclonal Antibody (Cat# AAA19991)

Full Name

Anti-SHKBP1 Antibody Picoband

Gene Names

SHKBP1; Sb1; PP203

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

< >

<< Previous Next >>

WB (Western Blot) (WB Suggested Anti-NFATC3 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:62500Positive Control: Hela cell lysate)

WB (Western Blot) (Host: RabbitTarget Name: NFATC3Sample Type: Fetal Lung lysatesAntibody Dilution: 1.0ug/ml)

WB (Western Blot) (Host: MouseTarget Name: NFATC3Sample Tissue: Mouse TestisAntibody Dilution: 1ug/ml)

WB (Western Blot) (Host: MouseTarget Name: NFATC3Sample Tissue: Mouse PancreasAntibody Dilution: 1ug/ml)

IHC (Immunohistochemistry) (Rabbit Anti-NFATC3 AntibodyParaffin Embedded Tissue: Human alveolar cellCellular Data: Epithelial cells of renal tubuleAntibody Concentration: 4.0-8.0 ug/mlMagnification: 400X)

IHC (Immunohistochemistry) (Immunohistochemistry with Thymus tissue at an antibody concentration of 5ug/ml using anti-NFATC3 antibody)

Compare (5 max)

NFATC3, Polyclonal Antibody (Cat# AAA23612)

Full Name

NFATC3 antibody - N-terminal region

Gene Names

NFATC3; NFAT4; NFATX; NF-AT4c

Reactivity

Cow, Dog, Horse, Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purified

Pricing

$530/0.1 mL $1,005/2x0.1 mL $1,455/3x0.1 mL $1,885/4x0.1 mL $2,140/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using Acetyl-Histone H3-K9 antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using Acetyl-Histone H3-K9 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using Acetyl-Histone H3-K9 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat ovary using Acetyl-Histone H3-K9 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon using Acetyl-Histone H3-K9 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human mammary cancer using Acetyl-Histone H3-K9 antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using Acetyl-Histone H3-K9 antibody.)

Compare (5 max)

Histone H3-K9, Polyclonal Antibody (Cat# AAA29842)

Full Name

Acetyl-Histone H3-K9 pAb

Gene Names

HIST3H3; H3t; H3.4; H3/g; H3FT

Reactivity

Human, Mouse, Rat, Other (Wide Range)

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation, Chromatin Immunoprecipitation, Chromatin Immunoprecipitation

Purity

Affinity purification

Pricing

$360/0.05 mL $450/0.1 mL $855/2x0.1 mL $1,230/3x0.1 mL $1,595/4x0.1 mL $1,750/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

Compare (5 max)

KLF13, Polyclonal Antibody (Cat# AAA29223)

Full Name

KLF13 Polyclonal Antibody

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

Compare (5 max)

GNL3L, Polyclonal Antibody (Cat# AAA29031)

Full Name

GNL3L Polyclonal Antibody

Gene Names

GNL3L; RP11-353K22.1

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistchemistry-Paraffin) (Paraformaldehyde-fixed, paraffin embedded (human kidney); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (C4orf52) Polyclonal Antibody, Unconjugated (bs-15196R) at 1:400 overnight at 4 degree C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.)

IHC (Immunohistochemistry) (Paraformaldehyde-fixed, paraffin embedded (rat stomach); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (C4orf52) Polyclonal Antibody, Unconjugated (bs-15196R) at 1:400 overnight at 4 degree C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.)

IHC (Immunohistochemistry-Paraffin) (Paraformaldehyde-fixed, paraffin embedded (rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37 degree C for 30min; Antibody incubation with (C4orf52) Polyclonal Antibody, Unconjugated (bs-15196R) at 1:400 overnight at 4 degree C, followed by a conjugated secondary (sp-0023) for 20 minutes and DAB staining.)

WB (Western Blot) (Dilution: Sample: heart (Mouse) Lysate at 40 ug Primary: Anti-C4orf52(bs-15196R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18 kD Observed band size: 18 kD)

WB (Western Blot) (Sample: muscle (Mouse) Lysate at 40 ug Primary: Anti-C4orf52(bs-15196R) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 18 kD Observed band size: 18 kD)

IHC (Immunohistochemistry-Paraffin) (Tissue/cell: Rat kidney tissue; 4% Paraformaldehyde-fixed and paraffin-embedded; Antigen retrieval: citrate buffer ( 0.01M, pH 6.0 ), Boiling bathing for 15min; Block endogenous peroxidase by 3% Hydrogen peroxide for 30min; Blocking buffer (normal goat serum,C-0005) at 37? for 20 min; Incubation: Anti-C4orf52 Polyclonal Antibody, Unconjugated(bs-15196R) 1:5000, overnight at 4?C, followed by conjugation to the secondary antibody(SP-0023) and DAB(C-0010) staining)

Compare (5 max)

C4orf52, Polyclonal Antibody (Cat# AAA28817)

Full Name

C4orf52 Polyclonal Antibody

Gene Names

SMIM20; C4orf52

Reactivity

Rat, Dog, Cow

Applications

Western Blot, Immunohistochemistry, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using SUMO1 antibody (AAA28442) at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using SUMO1 antibody (AAA28442) at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using SUMO1 antibody (AAA28442) at dilution of 1:100. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded mouse testis using SUMO1 antibody (AAA28442) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded rat brain using SUMO1 antibody (AAA28442) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human lung cancer using SUMO1 antibody (AAA28442) at dilution of 1:200 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of extracts from wild type(WT) and SUMO1 Rabbit pAb knockout (KO) 293T cells, using SUMO1 Rabbit pAb antibody (AAA28442) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit (RM00021). Exposure time: 180s.)

Compare (5 max)

SUMO1, Polyclonal Antibody (Cat# AAA28442)

Full Name

[KO Validated] SUMO1 Rabbit pAb

Gene Names

SUMO1; DAP1; GMP1; PIC1; SMT3; UBL1; OFC10; SENP2; SMT3C; SMT3H3

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Affinity purification

Pricing

$285/0.02 mL $500/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,780/4x0.1 mL $1,960/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded rat liver using WBP11 Rabbit pAb.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse lung using WBP11 Rabbit pAb.)

IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using WBP11 Rabbit pAb.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using WBP11 Rabbit pAb.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using WBP11 Rabbit pAb.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using WBP11 antibody.)

Compare (5 max)

WBP11, Polyclonal Antibody (Cat# AAA30635)

Full Name

WBP11 Rabbit Polyclonal Antibody

Gene Names

WBP11; NPWBP; SIPP1; WBP-11; PPP1R165

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

$360/0.05 mL $450/0.1 mL $855/2x0.1 mL $1,230/3x0.1 mL $1,595/4x0.1 mL $1,750/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded rat pancreas using LMNB2 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using LMNB2 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using LMNB2 Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using LMNB2 Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using LMNB2 Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver injury using LMNB2 Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat pancreas using LMNB2 Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using LMNB2 Antibody at dilution of 1:200 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using LMNB2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)

Compare (5 max)

LMNB2, Polyclonal Antibody (Cat# AAA28186)

Full Name

LMNB2 Polyclonal Antibody

Gene Names

LMNB2; LMN2; LAMB2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse brain using ADAR antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse testis using ADAR antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human stomach using ADAR antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human breast cancer using ADAR antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver using ADAR antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat heart using ADAR antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using ADAR antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat testis using ADAR antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using ADAR antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 20s.)

Compare (5 max)

ADAR, Polyclonal Antibody (Cat# AAA28202)

Full Name

ADAR Polyclonal Antibody

Gene Names

ADAR; DSH; AGS6; G1P1; IFI4; P136; ADAR1; DRADA; DSRAD; IFI-4; K88DSRBP

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of SiHa cells using anti-TARS2 antibody (AAA19627).Overlay histogram showing SiHa cells stained with AAA19627 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TARS2 Antibody (AAA19627, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 6. IF analysis of TARS2 using anti-TARS2 antibody (AAA19627).TARS2 was detected in an immunocytochemical section of T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-TARS2 Antibody (AAA19627) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of TARS2 using anti-TARS2 antibody (AAA19627).TARS2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-TARS2 Antibody (AAA19627) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of TARS2 using anti-TARS2 antibody (AAA19627).TARS2 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-TARS2 Antibody (AAA19627) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of TARS2 using anti-TARS2 antibody (AAA19627).TARS2 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-TARS2 Antibody (AAA19627) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of TARS2 using anti-TARS2 antibody (AAA19627).TARS2 was detected in a paraffin-embedded section of human colonic adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-TARS2 Antibody (AAA19627) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of TARS2 using anti-TARS2 antibody (AAA19627).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human HepG2 whole cell lysates,Lane 2: human Hela whole cell lysates,Lane 3: human THP-1 whole cell lysates,Lane 4: human Raji whole cell lysates,Lane 5: rat liver tissue lysates,Lane 6: mouse liver tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TARS2 antigen affinity purified polyclonal antibody (#AAA19627) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TARS2 at approximately 72 kDa. The expected band size for TARS2 is at 72 kDa.)

Compare (5 max)

TARS2, Polyclonal Antibody (Cat# AAA19627)

Full Name

Anti-TARS2 Antibody Picoband

Gene Names

TARS2; thrRS; TARSL1; COXPD21

Reactivity

Human, Mouse, Rat

Applications

EIA, FC/FACS, IF, IHC, ICC, WB

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of Caco-2 cells using anti-CD14 antibody (AAA19391).Overlay histogram showing Caco-2 cells stained with AAA19391 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CD14 Antibody (AAA19391, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 6. IF analysis of CD14 using anti-CD14 antibody (AAA19391).CD14 was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-CD14 Antibody (AAA19391) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of CD14 using anti-CD14 antibody (AAA19391).CD14 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-CD14 Antibody (AAA19391) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of CD14 using anti-CD14 antibody (AAA19391).CD14 was detected in a paraffin-embedded section of human renal cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-CD14 Antibody (AAA19391) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of CD14 using anti-CD14 antibody (AAA19391).CD14 was detected in a paraffin-embedded section of human lymphadenoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-CD14 Antibody (AAA19391) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of CD14 using anti-CD14 antibody (AAA19391).CD14 was detected in a paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-CD14 Antibody (AAA19391) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 1. IHC analysis of CD14 using anti-CD14 antibody (AAA19391).CD14 was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-CD14 Antibody (AAA19391) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

Compare (5 max)

CD14, Polyclonal Antibody (Cat# AAA19391)

Full Name

Anti-CD14 Antibody Picoband

Reactivity

Human

Applications

EIA, FC/FACS, IF, IHC, ICC

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 10. Flow Cytometry analysis of PC-3 cells using anti-MLX-interacting protein/MLXIP antibody (AAA19603).Overlay histogram showing PC-3 cells stained with AAA19603 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MLX-interacting protein/MLXIP Antibody (AAA19603, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 9. IF analysis of MLX-interacting protein/MLXIP using anti-MLX-interacting protein/MLXIP antibody (AAA19603).MLX-interacting protein/MLXIP was detected in an immunocytochemical section of SiHa cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-MLX-interacting protein/MLXIP Antibody (AAA19603) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 8. IHC analysis of MLX-interacting protein/MLXIP using anti-MLX-interacting protein/MLXIP antibody (AAA19603).MLX-interacting protein/MLXIP was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-MLX-interacting protein/MLXIP Antibody (AAA19603) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of MLX-interacting protein/MLXIP using anti-MLX-interacting protein/MLXIP antibody (AAA19603).MLX-interacting protein/MLXIP was detected in a paraffin-embedded section of rat colon tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-MLX-interacting protein/MLXIP Antibody (AAA19603) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of MLX-interacting protein/MLXIP using anti-MLX-interacting protein/MLXIP antibody (AAA19603).MLX-interacting protein/MLXIP was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-MLX-interacting protein/MLXIP Antibody (AAA19603) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of MLX-interacting protein/MLXIP using anti-MLX-interacting protein/MLXIP antibody (AAA19603).MLX-interacting protein/MLXIP was detected in a paraffin-embedded section of human laryngeal squamous cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-MLX-interacting protein/MLXIP Antibody (AAA19603) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of MLX-interacting protein/MLXIP using anti-MLX-interacting protein/MLXIP antibody (AAA19603).MLX-interacting protein/MLXIP was detected in a paraffin-embedded section of human hyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-MLX-interacting protein/MLXIP Antibody (AAA19603) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of MLX-interacting protein/MLXIP using anti-MLX-interacting protein/MLXIP antibody (AAA19603).MLX-interacting protein/MLXIP was detected in a paraffin-embedded section of human appendiceal adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-MLX-interacting protein/MLXIP Antibody (AAA19603) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of MLX-interacting protein/MLXIP using anti-MLX-interacting protein/MLXIP antibody (AAA19603).MLX-interacting protein/MLXIP was detected in a paraffin-embedded section of human thyroid papillary carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-MLX-interacting protein/MLXIP Antibody (AAA19603) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of MLX-interacting protein/MLXIP using anti-MLX-interacting protein/MLXIP antibody (AAA19603).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human HepG2 whole cell lysates,Lane 2: human K562 whole cell lysates,Lane 3: human HEK293 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MLX-interacting protein/MLXIP antigen affinity purified polyclonal antibody (#AAA19603) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MLX-interacting protein/MLXIP at approximately 100 kDa. The expected band size for MLX-interacting protein/MLXIP is at 100 kDa.)

Compare (5 max)

MLX-interacting protein/MLXIP, Polyclonal Antibody (Cat# AAA19603)

Full Name

Anti-MLX-interacting protein/MLXIP Antibody Picoband

Gene Names

MLXIP; MIR; MONDOA; bHLHe36

Reactivity

Human, Rat

Applications

EIA, FC/FACS, IF, IHC, ICC, WB

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Figure 8. IHC analysis of RbAp48 using anti-RbAp48 antibody (AAA11671).RbAp48 was detected in frozen section of rat small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-RbAp48 Antibody (AAA11671) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of RbAp48 using anti-RbAp48 antibody (AAA11671).RbAp48 was detected in frozen section of mouse small intestine tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-RbAp48 Antibody (AAA11671) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of RbAp48 using anti-RbAp48 antibody (AAA11671).RbAp48 was detected in frozen section of mouse liver tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-RbAp48 Antibody (AAA11671) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of RbAp48 using anti-RbAp48 antibody (AAA11671).RbAp48 was detected in frozen section of human placenta tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-RbAp48 Antibody (AAA11671) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of RbAp48 using anti-RbAp48 antibody (AAA11671).RbAp48 was detected in paraffin-embedded section of Human Intestinal Cancer Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-RbAp48 Antibody (AAA11671) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of RbAp48 using anti-RbAp48 antibody (AAA11671).RbAp48 was detected in paraffin-embedded section of Rat Intestine Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-RbAp48 Antibody (AAA11671) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of RbAp48 using anti-RbAp48 antibody (AAA11671).RbAp48 was detected in paraffin-embedded section of Mouse Liver Tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1ug/ml rabbit anti-RbAp48 Antibody (AAA11671) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of RbAp48 using anti-RbAp48 antibody (AAA11671).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: Rat Brain Tissue Lysate,Lane 2: Mouse Liver Tissue Lysate,Lane 3: Mouse Lung Tissue Lysate,Lane 4: HELA Whole Cell Lysate,Lane 5: JURKAT Whole Cell Lysate.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RbAp48 antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RbAp48 at approximately 54KD. The expected band size for RbAp48 is at 54KD.)

Compare (5 max)

RbAp48, Polyclonal Antibody (Cat# AAA11671)

Full Name

Anti-RbAp48 Antibody

Gene Names

RBBP4; NURF55; RBAP48; lin-53

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Immunogen Affinity Purified

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human stomach using MAPKBP1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse stomach using MAPKBP1 antibody (AAA10759) at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using MAPKBP1 antibody (AAA10759) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.)

Compare (5 max)

MAPKBP1, Polyclonal Antibody (Cat# AAA10759)

Full Name

MAPKBP1 Polyclonal Antibody

Gene Names

MAPKBP1; JNKBP-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 8. Flow Cytometry analysis of HepG2 cells using anti-MERTK antibody (AAA19219).Overlay histogram showing HepG2 cells stained with AAA19219 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MERTK Antibody (AAA19219, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG (5-10ug/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 7. IHC analysis of MERTK using anti-MERTK antibody (AAA19219).MERTK was detected in a paraffin-embedded section of human retal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MERTK Antibody (AAA19219) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of MERTK using anti-MERTK antibody (AAA19219).MERTK was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MERTK Antibody (AAA19219) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of MERTK using anti-MERTK antibody (AAA19219).MERTK was detected in a paraffin-embedded section of human lymphoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MERTK Antibody (AAA19219) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of MERTK using anti-MERTK antibody (AAA19219).MERTK was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MERTK Antibody (AAA19219) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen)

IHC (Immunohistochemistry) (Formalin-fixed and paraffin-embedded human lymphoma reacted with MERTK Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of MERTK using anti-MERTK antibody (AAA19219).MERTK was detected in a paraffin-embedded section of human gallbladder adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-MERTK Antibody (AAA19219) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of MERTK using anti-MERTK antibody (AAA19219).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human HepG2 whole cell lysatesLane 2: human Jurkat whole cell lysatesLane 3: human Caco-2 whole cell lysatesLane 4: rat testis tissue lysatesLane 5: rat lung tissue lysatesLane 7: mouse testis tissue lysatesLane 8: mouse lung tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-MERTK antigen affinity purified polyclonal antibody (Catalog # AAA19219) at 0. 5ug/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for MERTK at approximately 120 kDa. The expected band size for MERTK is at 120 kDa.)

Compare (5 max)

MERTK, Polyclonal Antibody (Cat# AAA19219)

Full Name

Anti-MERTK Antibody

Gene Names

MERTK; MER; RP38; c-mer

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry, Direct ELISA

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of MCF-7 cells using anti-Epac1/RAPGEF3 antibody (AAA19459).Overlay histogram showing MCF-7 cells stained with AAA19459 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Epac1/RAPGEF3 Antibody (AAA19459, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of Epac1/RAPGEF3 using anti-Epac1/RAPGEF3 antibody (AAA19459).Epac1/RAPGEF3 was detected in an immunocytochemical section of T-47D cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-Epac1/RAPGEF3 Antibody (AAA19459) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of Epac1/RAPGEF3 using anti-Epac1/RAPGEF3 antibody (AAA19459).Epac1/RAPGEF3 was detected in a paraffin-embedded section of human rupture of spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Epac1/RAPGEF3 Antibody (AAA19459) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of Epac1/RAPGEF3 using anti-Epac1/RAPGEF3 antibody (AAA19459).Epac1/RAPGEF3 was detected in a paraffin-embedded section of human pancreatic cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Epac1/RAPGEF3 Antibody (AAA19459) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of Epac1/RAPGEF3 using anti-Epac1/RAPGEF3 antibody (AAA19459).Epac1/RAPGEF3 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Epac1/RAPGEF3 Antibody (AAA19459) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of Epac1/RAPGEF3 using anti-Epac1/RAPGEF3 antibody (AAA19459).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human HepG2 whole cell lysates,Lane 3: monkey COS7 whole cell lysates,Lane 4: human T-47D whole cell lysates,Lane 5: mouse RAW264.7 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Epac1/RAPGEF3 antigen affinity purified polyclonal antibody (#AAA19459) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Epac1/RAPGEF3 at approximately 104 kDa. The expected band size for Epac1/RAPGEF3 is at 104 kDa.)

Compare (5 max)

Epac1/RAPGEF3, Polyclonal Antibody (Cat# AAA19459)

Full Name

Anti-Epac1/RAPGEF3 Antibody Picoband

Gene Names

RAPGEF3; EPAC; EPAC1; bcm910; HSU79275; CAMP-GEFI

Reactivity

Human, Mouse, Monkey

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using YAP1 Rabbit pAb.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse lung using YAP1 Rabbit pAb.)

IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using YAP1 Rabbit pAb.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat spleen using YAP1 Rabbit pAb.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung squamous carcinoma tissue using YAP1 Rabbit pAb.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using YAP1 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human extranodal NK-T cell lymphoma using YAP1 Rabbit pAb.)

Compare (5 max)

YAP1, Polyclonal Antibody (Cat# AAA30658)

Full Name

YAP1 Rabbit Polyclonal Antibody

Gene Names

YAP1; YAP; YKI; YAP2; YAP65

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

$360/0.05 mL $450/0.1 mL $855/2x0.1 mL $1,230/3x0.1 mL $1,595/4x0.1 mL $1,750/5x0.1 mL

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 9. Flow Cytometry analysis of HepG2 cells using anti-Mitofilin/IMMT antibody (AAA19806).Overlay histogram showing HepG2 cells stained with AAA19806 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Mitofilin/IMMT Antibody (AAA19806, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 8. IF analysis of Mitofilin/IMMT using anti-Mitofilin/IMMT antibody (AAA19806).Mitofilin/IMMT was detected in an immunocytochemical section of HELA cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Mitofilin/IMMT Antibody (AAA19806) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Mitofilin/IMMT Antibody (AAA19806) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Mitofilin/IMMT Antibody (AAA19806) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Mitofilin/IMMT Antibody (AAA19806) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Mitofilin/IMMT Antibody (AAA19806) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-Mitofilin/IMMT Antibody (AAA19806) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human HepG2 whole cell lysates,Lane 3: rat NRK whole cell lysates,Lane 4: rat PC-12 whole cell lysates,Lane 5: rat C6 whole cell lysates,Lane 6: mouse HEPA1/6 whole cell lysates,Lane 7: mouse MFC whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Mitofilin/IMMT antigen affinity purified polyclonal antibody (#AAA19806) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Mitofilin/IMMT at approximately 80-90 kDa. The expected band size for Mitofilin/IMMT is at 84 kDa.)

Compare (5 max)

Mitofilin/IMMT, Polyclonal Antibody (Cat# AAA19806)

Full Name

Anti-Mitofilin/IMMT Antibody Picoband

Gene Names

IMMT; HMP; P87; P89; PIG4; PIG52; MINOS2; P87/89

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, ICC, IF, FC/FACS, EIA

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1:100-300 ELISA: 1/20000. Not yet tested in other applications.)

Compare (5 max)

Mcl-1, Polyclonal Antibody (Cat# AAA29161)

Full Name

Mcl-1 Polyclonal Antibody

Gene Names

MCL1; TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT

Reactivity

Human, Mouse, Rat

Applications

WB, IHC-P, IF

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

< >

<< Previous Next >>

Transfection Control (Anti-XPR1 antibody immunocytochemistry (ICC) staining of untransfected HEK293 human embryonic kidney cells.)

Transfection (Anti-XPR1 antibody immunocytochemistry (ICC) staining of HEK293 human embryonic kidney cells transfected with XPR1.)

IHC (Immunohistochemistry) (Anti-XPR1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemistry) (Anti-XPR1 antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemistry) (Anti-XPR1 antibody IHC of human Pancreas, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

IHC (Immunohistochemistry) (Anti-XPR1 antibody IHC of human Breast, Carcinoma. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval.)

Compare (5 max)

XPR1, Polyclonal Antibody (Cat# AAA12332)

Full Name

Rabbit Polyclonal to Human XPR1

Gene Names

XPR1; X3; SYG1

Reactivity

Gibbon, Hamster, Human, Monkey, Mouse, Rabbit
Predicted Reactivity: Rat, Bovine, Dog, Horse, Pig (at least 90% immunogen sequence identity)

Applications

Immunohistochemistry, Immunocytochemistry

Purity

Immunoaffinity Purified

Pricing

$690/0.05 mg $1,325/2x0.05 mg $1,915/3x0.05 mg $2,475/4x0.05 mg $2,800/5x0.05 mg

< >

<< Previous Next >>

WB (Western Blot) (WB Suggested Anti-SENP3 Antibody Titration: 0.2-1 ug/mlELISA Titer: 1:1562500Positive Control: 721_B cell lysateSENP3 is strongly supported by BioGPS gene expression data to be expressed in Human 721_B cells)

WB (Western Blot) (Sample Type: 1. HeLa cell extract (200ug)Primary Dilution: 1:1000Secondary Antibody: alkaline phosphatase-conjugated anti-rabbitSecondary Dilution: 1:1000Image Submitted by: Andreia CarvalhoIBMC-OBF, Portugal.SENP3 is strongly supported by BioGPS gene expression data to be expressed in HeLa)

WB (Western Blot) (Sample Type: 1. HeLa cell extract (200ug)2. HeLa cell extract (200ug) incubated with HA-SUMO2-VME*HA-S2-VME-SENP3Primary Dilution: 1:1000Secondary Antibody: alkaline phosphatase-conjugated anti-rabbitSecondary Dilution: 1:1000Image Submitted by: Andreia CarvalhoIBMC-OBF, Portugal.SENP3 is strongly supported by BioGPS gene expression data to be expressed in HeLa)

WB (Western Blot) (Sample Type: RatSample Type: 1. Rat Liver (200ug)2. Rat Brain (200ug)3. Rat Testis (200ug)4. Rat Kidney (200ug)5.Rat Liver nuclei (-) no HA-SUMO2-WME6.Rat Liver nuclei (+) incubated with 100 ng HA-SUMO2-WMEPrimary Dilution: 1:1000Secondary Antibody: alkaline phosphatase-conjugated anti-rabbitSecondary Dilution: 1:1000Image Submitted by: Andreia CarvalhoIBMC-OBF, PortugalSee Customer Feedback tab for detailed information.)

IHC (Immunohistochemistry) (SENP3 antibody - N-terminal region in HeLa cells using Immunofluorescence.SENP3 is strongly supported by BioGPS gene expression data to be expressed in HeLa)

IF (Immunofluorescence) (SENP3 antibody - N-terminal region in HeLa cells using Immunofluorescence.Antibody concentration: 2 ug/mlData submitted by: Michael Matunis, Johns Hopkins UniversitySENP3 is strongly supported by BioGPS gene expression data to be expressed in HeLa)

Compare (5 max)

SENP3, Polyclonal Antibody (Cat# AAA23562)

Full Name

SENP3 antibody - N-terminal region

Gene Names

SENP3; SSP3; Ulp1; SMT3IP1

Reactivity

Cow, Dog, Guinea Pig, Horse, Human, Mouse, Rabbit, Rat

Applications

Immunofluorescence, Western Blot

Purity

Affinity Purified

Pricing

$530/0.1 mL $1,005/2x0.1 mL $1,455/3x0.1 mL $1,885/4x0.1 mL $2,140/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

Application Data (Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

Compare (5 max)

GDF-6, Polyclonal Antibody (Cat# AAA29313)

Full Name

GDF-6 Polyclonal Antibody

Gene Names

GDF6; KFM; KFS; KFS1; KFSL; SGM1; BMP13; CDMP2; MCOP4; SCDO4; MCOPCB6

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse spleen using PAXIP1 Rabbit pAb at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse brain using PAXIP1 Rabbit pAb at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast cancer using PAXIP1 Rabbit pAb at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human thyroid cancer using PAXIP1 Rabbit pAb at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat spleen using PAXIP1 Rabbit pAb at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat ovary using PAXIP1 Rabbit pAb at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using PAXIP1 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 180s.)

Compare (5 max)

PAXIP1, Polyclonal Antibody (Cat# AAA28350)

Full Name

PAXIP1 Rabbit pAb

Gene Names

PAXIP1; PTIP; TNRC2; CAGF28; CAGF29; PACIP1; PAXIP1L

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Affinity purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Dilution: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

Compare (5 max)

RGS16, Polyclonal Antibody (Cat# AAA29109)

Full Name

RGS16 Polyclonal Antibody

Gene Names

RGS16; RGS-R; A28-RGS14; A28-RGS14P

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunofluorescence

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using HNRNPM at 1:1000 dilution.)

IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using hnRNPM at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of L929 cells using hnRNPM at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using hnRNPM at dilution of 1:100. Blue: DAPI for nuclear staining.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse heart using HNRNPM at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using HNRNPM at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human esophageal cancer using HNRNPM at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using HNRNPM at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using HNRNPM at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using HNRNPM at dilution of 1:100 (40x lens).)

Compare (5 max)

HNRNPM, Polyclonal Antibody (Cat# AAA30681)

Full Name

HNRNPM Rabbit Polyclonal Antibody

Gene Names

HNRNPM; CEAR; HNRPM; HTGR1; NAGR1; HNRPM4; HNRNPM4; hnRNP M

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

$360/0.05 mL $450/0.1 mL $855/2x0.1 mL $1,230/3x0.1 mL $1,595/4x0.1 mL $1,750/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using NASP at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human stomach using NASP at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast cancer using NASP at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast using NASP at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human brain astrocytoma using NASP at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human appendicitis using NASP at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human tonsil using NASP at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat stomach using NASP at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat spleen using NASP at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using NASP at 1:1000 dilution.)

Compare (5 max)

NASP, Polyclonal Antibody (Cat# AAA30682)

Full Name

NASP Rabbit Polyclonal Antibody

Gene Names

NASP; FLB7527; PRO1999

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

$360/0.05 mL $450/0.1 mL $855/2x0.1 mL $1,230/3x0.1 mL $1,595/4x0.1 mL $1,750/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of rat skin using Cytokeratin 16 (KRT16) Rabbit pAb.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human skin using Cytokeratin 16 (KRT16) Rabbit pAb.)

IF (Immunofluorescence) (Immunofluorescence analysis of mouse skin using Cytokeratin 16 (KRT16) Rabbit pAb.)

IF (Immunofluorescence) (Immunofluorescence analysis of A-549 cells using Cytokeratin 16 (KRT16) Rabbit pAb.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human esophageal cancer using Cytokeratin 16 (KRT16) Rabbit pAb.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using Cytokeratin 16 (KRT16) antibody.)

Compare (5 max)

KRT16, Polyclonal Antibody (Cat# AAA30690)

Full Name

KRT16 Rabbit Polyclonal Antibody

Gene Names

KRT16; K16; CK16; K1CP; NEPPK; FNEPPK; KRT16A

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

$360/0.05 mL $450/0.1 mL $855/2x0.1 mL $1,230/3x0.1 mL $1,595/4x0.1 mL $1,750/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using FKBP4 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human adenomyosis using FKBP4 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using FKBP4 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using FKBP4 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human adenomyosis using FKBP4 Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human leiomyoma of uterus using FKBP4 Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using FKBP4 Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver injury using FKBP4 Antibody at dilution of 1:200 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using FKBP4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)

Compare (5 max)

FKBP4, Polyclonal Antibody (Cat# AAA28189)

Full Name

FKBP4 Polyclonal Antibody

Gene Names

FKBP4; HBI; p52; Hsp56; FKBP51; FKBP52; FKBP59; PPIase

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistchemistry) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: IHC-p: 100-300.Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

Compare (5 max)

IL-3Ralpha, Polyclonal Antibody (Cat# AAA29300)

Full Name

IL-3Ralpha Polyclonal Antibody

Gene Names

IL3RA; IL3R; CD123; IL3RX; IL3RY; IL3RAY; hIL-3Ra

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of MCF7 cells using MYO1C antibody.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using MYO1C antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse liver using MYO1C antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human esophagus using MYO1C antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver damage using MYO1C antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using MYO1C antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using MYO1C antibody.)

Compare (5 max)

MYO1C, Polyclonal Antibody (Cat# AAA29832)

Full Name

MYO1C Polyclonal Antibody

Gene Names

MYO1C; NMI; MMIb; myr2; MMI-beta

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

$360/0.05 mL $450/0.1 mL $855/2x0.1 mL $1,230/3x0.1 mL $1,595/4x0.1 mL $1,750/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of JNK1 on hybrid fish (crucian-carp) brain tissue lysate using anti-JNK1 antibody at 1/500 dilution.)

ICC (Immunocytochemistry) (ICC staining of BNDF in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining of BNDF in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining of BNDF in A172 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-BDNF antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-BDNF antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-BDNF antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-BDNF antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of JNK1 on different cell lysates using anti-JNK1 antibody at 1/1, 000 dilution. Positive control: Lane 1: K562     Lane 2: PC-12 Lane 3: Hela)

Compare (5 max)

JNK1, Polyclonal Antibody (Cat# AAA29866)

Full Name

JNK1 Antibody

Gene Names

MAPK8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2

Reactivity

Human, Mouse, Rat, Zebrafish

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified

Pricing

$390/0.05 mL $510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,015/5x0.1 mL

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of HEPA1-6 cells using anti-Rad9b antibody (AAA19340).Overlay histogram showing HEPA1-6 cells stained with AAA19340 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Rad9b Antibody (AAA19340, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of Rad9b using anti-Rad9b antibody (AAA19340).Rad9b was detected in paraffin-embedded section of rat cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Rad9b Antibody (AAA19340) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of Rad9b using anti-Rad9b antibody (AAA19340).Rad9b was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Rad9b Antibody (AAA19340) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of Rad9b using anti-Rad9b antibody (AAA19340).Rad9b was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Rad9b Antibody (AAA19340) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of Rad9b using anti-Rad9b antibody (AAA19340).Rad9b was detected in paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Rad9b Antibody (AAA19340) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of Rad9b using anti-Rad9b antibody (AAA19340).Rad9b was detected in paraffin-embedded section of mouse cardiac muscle tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 1μg/ml rabbit anti-Rad9b Antibody (AAA19340) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (Catalog # with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of Rad9b using anti-Rad9b antibody (AAA19340).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: rat RH35 whole cell lysatesLane 2: mouse NIH/3T3 whole cell lysatesLane 3: mouse SP2/0 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Rad9b antigen affinity purified polyclonal antibody (Catalog # AAA19340) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # with Tanon 5200 system. A specific band was detected for Rad9b at approximately 60KD. The expected band size for Rad9b is at 60KD.)

Compare (5 max)

Rad9b, Polyclonal Antibody (Cat# AAA19340)

Full Name

Anti-Rad9b Antibody

Gene Names

Rad9b; BC021784; A630082N15Rik

Reactivity

Mouse, Rat

Applications

WB, IHC-P, FC/FACS/FCM, EIA

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using Phospho-Histone H3-S10/T11 Rabbit pAb at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using Phospho-Histone H3-S10/T11 Rabbit pAb at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using Phospho-Histone H3-S10/T11 Rabbit pAb at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using Phospho-Histone H3-S10/T11 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat liver using Phospho-Histone H3-S10/T11 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using Phospho-Histone H3-S10/T11 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of NIH/3T3 cells, using phospho-STK4-T387 pAb at 1:1000 dilution or Histone H3 antibody (A15741).HeLa, NIH/3T3 and C6 cellls were treated by nocodazole (50 ng/mL) at 37℃ for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using Phospho-Histone H3-S10/T11 antibody at 1:1000 dilution. Both NIH/3T3 cells and Hela cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour and treated by nocodazole (50 ng/mL) at 37℃ for 20 hours.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% BSA.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)

Compare (5 max)

Phospho-Histone H3-S10/T11, Polyclonal Antibody (Cat# AAA28391)

Full Name

Phospho-Histone H3-S10/T11 Rabbit pAb

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse, Rat, Other (Wide Range)

Applications

WB, IHC, IF

Purity

Affinity purification

Pricing

$285/0.02 mL $500/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,780/4x0.1 mL $1,960/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using NDUFB8 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using NDUFB8 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of A-549 cells using NDUFB8 Polyclonal Antibody at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using NDUFB8 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver using NDUFB8 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines using NDUFB8 Polyclonal Antibody at 1:500 dilution.)

Compare (5 max)

NDUFB8, Polyclonal Antibody (Cat# AAA22274)

Full Name

NDUFB8 Polyclonal Antibody

Gene Names

NDUFB8; ASHI; CI-ASHI

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

$330/0.06 mL $445/0.12 mL $675/0.2 mL $1,290/2x0.2 mL $1,875/3x0.2 mL $2,420/4x0.2 mL $2,905/5x0.2 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human-breast-cancer, antibody was diluted at 1:200)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human-breast-cancer, antibody was diluted at 1:200)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human-liver-cancer, antibody was diluted at 1:200)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human-liver-cancer, antibody was diluted at 1:200)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:200(4 degree overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:200(4 degree overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1, Antibody was diluted at 1:200(4 degree overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:200(4 degree overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:200(4 degree overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human colon. 1, Antibody was diluted at 1:200(4 degree overnight). 2, High-pressure and temperature EDTA, pH8.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)

Compare (5 max)

HDGF, Polyclonal Antibody (Cat# AAA30763)

Full Name

HDGF Rabbit Polyclonal Antibody

Gene Names

HDGF; HMG1L2

Reactivity

Human, Rat, Mouse

Applications

Immunohistochemistry, Immunohistochemistry

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$335/0.05 mL $415/0.1 mL $785/2x0.1 mL $1,130/3x0.1 mL $1,455/4x0.1 mL $1,550/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using CAMK1 at 1:3000 dilution.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using CAMK1 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse spinal cord using CAMK1 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse testis using CAMK1 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse lung using CAMK1 at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human stomach using CAMK1 at dilution of 1:100 (40x lens).)

Compare (5 max)

CAMK1, Polyclonal Antibody (Cat# AAA30624)

Full Name

CAMK1 Rabbit Polyclonal Antibody

Gene Names

CAMK1; CAMKI

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Affinity purification

Pricing

$360/0.05 mL $450/0.1 mL $855/2x0.1 mL $1,230/3x0.1 mL $1,595/4x0.1 mL $1,750/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Confocal immunofluorescence analysis of U2OS cells using LRPPRC Polyclonal at dilution of 1:400. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Confocal immunofluorescence analysis of HeLa cells using LRPPRC Polyclonal at dilution of 1:400. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Confocal immunofluorescence analysis of U-2 OS cells using LRPPRC Polyclonal at dilution of 1:400. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Confocal immunofluorescence analysis of Hela cells using LRPPRC Polyclonal at dilution of 1:400. Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using LRPPRC at 1:1000 dilution.)

IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using LRPPRC at dilution of 1:100. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human stomach using LRPPRC at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human esophagus using LRPPRC at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using LRPPRC at dilution of 1:100 (40x lens).)

Compare (5 max)

LRPPRC, Polyclonal Antibody (Cat# AAA30667)

Full Name

LRPPRC Rabbit Polyclonal Antibody

Gene Names

LRPPRC; LSFC; GP130; LRP130; CLONE-23970

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

$360/0.05 mL $450/0.1 mL $855/2x0.1 mL $1,230/3x0.1 mL $1,595/4x0.1 mL $1,750/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

Application Data (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. IHC-p: 1/100-1/300. ELISA: 1/20000. Not yet tested in other applications.)

Compare (5 max)

Jagged1, Polyclonal Antibody (Cat# AAA29243)

Full Name

Jagged1 Polyclonal Antibody

Gene Names

JAG1; AGS; AHD; AWS; HJ1; CD339; JAGL1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of HEL cells using anti-Septin 11/SEPTIN11 antibody (AAA19631).Overlay histogram showing HEL cells stained with AAA19631 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Septin 11/SEPTIN11 Antibody (AAA19631, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistchemistry) (Figure 6. IHC analysis of Septin 11/SEPTIN11 using anti-Septin 11/SEPTIN11 antibody (AAA19631).Septin 11/SEPTIN11 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Septin 11/SEPTIN11 Antibody (AAA19631) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of Septin 11/SEPTIN11 using anti-Septin 11/SEPTIN11 antibody (AAA19631).Septin 11/SEPTIN11 was detected in a paraffin-embedded section of human spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Septin 11/SEPTIN11 Antibody (AAA19631) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of Septin 11/SEPTIN11 using anti-Septin 11/SEPTIN11 antibody (AAA19631).Septin 11/SEPTIN11 was detected in a paraffin-embedded section of rat brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Septin 11/SEPTIN11 Antibody (AAA19631) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of Septin 11/SEPTIN11 using anti-Septin 11/SEPTIN11 antibody (AAA19631).Septin 11/SEPTIN11 was detected in a paraffin-embedded section of mouse brain tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Septin 11/SEPTIN11 Antibody (AAA19631) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of Septin 11/SEPTIN11 using anti-Septin 11/SEPTIN11 antibody (AAA19631).Septin 11/SEPTIN11 was detected in a paraffin-embedded section of human appendix adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Septin 11/SEPTIN11 Antibody (AAA19631) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of Septin 11/SEPTIN11 using anti-Septin 11/SEPTIN11 antibody (AAA19631).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human 293T whole cell lysates,Lane 3: rat brain tissue lysates,Lane 4: mouse barin tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Septin 11/SEPTIN11 antigen affinity purified polyclonal antibody (#AAA19631) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Septin 11/SEPTIN11 at approximately 49 kDa. The expected band size for Septin 11/SEPTIN11 is at 49 kDa.)

Compare (5 max)

Septin 11/SEPTIN11, Polyclonal Antibody (Cat# AAA19631)

Full Name

Anti-Septin 11/SEPTIN11 Antibody Picoband

Reactivity

Human, Mouse, Rat

Applications

WB, IHC, FC/FACS

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded human adenomyosis using LSP1 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using LSP1 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver using LSP1 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using LSP1 Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human adenomyosis using LSP1 Antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using LSP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 30s.)

Compare (5 max)

LSP1, Polyclonal Antibody (Cat# AAA28192)

Full Name

LSP1 Polyclonal Antibody

Gene Names

LSP1; WP34; pp52

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

< >

<< Previous Next >>

FCM (Flow Cytometry) (Flow cytometric analysis of SH-SY5Y cells with SHP2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)

ICC (Immunocytochemistry) (ICC staining SHP2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining SHP2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining SHP2 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-SHP2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-SHP2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cacner tissue using anti-SHP2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-SHP2 antibody. Counter stained with hematoxylin.)

Compare (5 max)

SHP-2, Polyclonal Antibody (Cat# AAA29928)

Full Name

SHP-2 Antibody

Gene Names

PTPN11; CFC; NS1; SHP2; BPTP3; PTP2C; PTP-1D; SH-PTP2; SH-PTP3

Reactivity

Human, Mouse, Rat

Applications

ICC, IHC, FC/FACS

Purity

Protein affinity purified

Pricing

$390/0.05 mL $510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,015/5x0.1 mL

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 7. Flow Cytometry analysis of K562 cells using anti-Nac1/NACC1 antibody (AAA19602).Overlay histogram showing K562 cells stained with AAA19602 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Nac1/NACC1 Antibody (AAA19602, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of Nac1/NACC1 using anti-Nac1/NACC1 antibody (AAA19602).Nac1/NACC1 was detected in an immunocytochemical section of MCF-7 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL rabbit anti-Nac1/NACC1 Antibody (AAA19602) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The tissue section was developed using Phalloidin-iFluor 555 Conjugated. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of Nac1/NACC1 using anti-Nac1/NACC1 antibody (AAA19602).Nac1/NACC1 was detected in a paraffin-embedded section of human the renal pelvis is squamous metaplasia tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Nac1/NACC1 Antibody (AAA19602) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of Nac1/NACC1 using anti-Nac1/NACC1 antibody (AAA19602).Nac1/NACC1 was detected in a paraffin-embedded section of human papillary carcinoma of the left breast tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Nac1/NACC1 Antibody (AAA19602) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of Nac1/NACC1 using anti-Nac1/NACC1 antibody (AAA19602).Nac1/NACC1 was detected in a paraffin-embedded section of human bladder epithelial carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Nac1/NACC1 Antibody (AAA19602) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of Nac1/NACC1 using anti-Nac1/NACC1 antibody (AAA19602).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human MCF-7 whole cell lysates,Lane 3: human Caco-2 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nac1/NACC1 antigen affinity purified polyclonal antibody (#AAA19602) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Nac1/NACC1 at approximately 68 kDa. The expected band size for Nac1/NACC1 is at 57 kDa.)

Compare (5 max)

Nac1/NACC1, Polyclonal Antibody (Cat# AAA19602)

Full Name

Anti-Nac1/NACC1 Antibody Picoband

Gene Names

NACC1; NAC1; BEND8; NAC-1; BTBD30; BTBD14B

Reactivity

Human

Applications

EIA, FC/FACS, IF, IHC, ICC, WB

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of MOLT-4 cells using anti-HSPA4 antibody (AAA19794).Overlay histogram showing MOLT-4 cells stained with AAA19794 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HSPA4 Antibody (AAA19794, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

FCM (Flow Cytometry) (Figure 5. Flow Cytometry analysis of 293T cells using anti-HSPA4 antibody (AAA19794).Overlay histogram showing 293T cells stained with AAA19794 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-HSPA4 Antibody (AAA19794, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of HSPA4 using anti-HSPA4 antibody (AAA19794).HSPA4 was detected in a paraffin-embedded section of mouse ovary tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HSPA4 Antibody (AAA19794) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-HSPA4 Antibody (AAA19794) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human K562 whole cell lysates,Lane 3: rat brain tissue lysates,Lane 4: rat C6 whole cell lysates,Lane 5: mouse brain tissue lysates,Lane 6: mouse NIH/3T3 whole cell lysates,Lane 7: mouse Raw264.7 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPA4 antigen affinity purified polyclonal antibody (#AAA19794) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (16 kDa.)

WB (Western Blot) (Figure 1. Western blot analysis of HSPA4 using anti-HSPA4 antibody (AAA19794).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human 293T whole cell lysates,Lane 2: human K562 whole cell lysates,Lane 3: rat brain tissue lysates,Lane 4: rat C6 whole cell lysates,Lane 6: mouse brain tissue lysates,Lane 7: mouse NIH/3T3 whole cell lysates,Lane 8: mouse Raw264.7 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-HSPA4 antigen affinity purified polyclonal antibody (#AAA19794) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-DyLight 647 Conjugated secondary antibody at a dilution of 1:2000 for 1.5 hour at RT. A specific band was detected for HSPA4 at approximately 110 kDa. The expected band size for HSPA4 is at 96,14 kDa.)

Compare (5 max)

HSPA4, Polyclonal Antibody (Cat# AAA19794)

Full Name

Anti-HSPA4 Antibody Picoband

Gene Names

HSPA4; RY; APG-2; HSPH2; hsp70; hsp70RY; HS24/P52

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

< >

<< Previous Next >>

FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of U20S cells using anti-GLI2 antibody (AAA19737).Overlay histogram showing U20S cells stained with AAA19737 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-GLI2 Antibody (AAA19737, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of GLI2 using anti-GLI2 antibody (AAA19737) and anti-Beta Tubulin antibody (M01857-3).GLI2 was detected in immunocytochemical section of U2OS cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GLI2 Antibody (AAA19737) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GLI2 Antibody (AAA19737) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit ( epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-GLI2 Antibody (AAA19737) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit (Lane 2: human A549 whole cell lysates,Lane 3: human U20S whole cell lysates,Lane 4: rat brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-GLI2 antigen affinity purified polyclonal antibody (#AAA19737) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for GLI2 at approximately 180-200 kDa. The expected band size for GLI2 is at 220 kDa.)

Compare (5 max)

GLI2, Polyclonal Antibody (Cat# AAA19737)

Full Name

Anti-GLI2 Antibody Picoband

Gene Names

GLI2; HPE9; PHS2; THP1; THP2

Reactivity

Human, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using STRN3 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human stomach using STRN3 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using STRN3 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using STRN3 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse lung using STRN3 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using STRN3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 10s.)

Compare (5 max)

STRN3, Polyclonal Antibody (Cat# AAA28082)

Full Name

STRN3 Polyclonal Antibody

Gene Names

STRN3; SG2NA

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse heart using COPB2 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using COPB2 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using COPB2 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat heart using COPB2 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using COPB2 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using COPB2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)

Compare (5 max)

COPB2, Polyclonal Antibody (Cat# AAA28102)

Full Name

COPB2 Polyclonal Antibody

Gene Names

COPB2; beta'-COP

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of Mouse brain using CAMK2G antibody at dilution of 1 : 100 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of Rat brain using CAMK2G antibody at dilution of 1 : 100 (40x lens). Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse heart using CAMK2G antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat heart using CAMK2G antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human stomach using CAMK2G antibody at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using CAMK2G antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)

Compare (5 max)

CAMK2G, Polyclonal Antibody (Cat# AAA28433)

Full Name

CAMK2G Rabbit pAb

Gene Names

BSG; M6; OK; 5F7; TCSF; CD147; EMMPRIN

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Affinity purification

Pricing

$285/0.02 mL $500/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,780/4x0.1 mL $1,960/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of MCF-7 cells using GAB1 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse spinal cord using GAB1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using GAB1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using GAB1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using GAB1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat brain using GAB1 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using GAB1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 80s.)

Compare (5 max)

GAB1, Polyclonal Antibody (Cat# AAA28080)

Full Name

GAB1 Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of GFP-RNF168 transgenic U2OS cells using TP53BP1 antibody. Green:GFP-RNF168 fusion protein expression for DNA damage marker.Blue: DAPI for nuclear staining.RNF168(GFP) can be used to mark cells damaged by UV-A laser for they always gather around DNA damage region.)

IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using TP53BP1 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse heart using TP53BP1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistchemistry) (Immunohistochemistry of paraffin-embedded mouse spinal cord using TP53BP1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast cancer using TP53BP1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using TP53BP1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human lung cancer using TP53BP1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat kidney using TP53BP1 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of A-431 cells, using TP53BP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

Compare (5 max)

TP53BP1, Polyclonal Antibody (Cat# AAA28106)

Full Name

TP53BP1 Polyclonal Antibody

Gene Names

TP53BP1; p202; 53BP1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

$255/0.02 mL $430/0.1 mL $820/2x0.1 mL $1,175/3x0.1 mL $1,510/4x0.1 mL $1,610/5x0.1 mL

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.