Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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IHC (Immunohistochemistry) (IGF1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human liver tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IGF1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

IHC (Immunohistochemistry) (IGF1 Antibody (C-term) immunohistochemistry analysis in formalin fixed and paraffin embedded human kidney tissue followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of IGF1 Antibody (C-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

FCM/FACS (Flow Cytometry) (IGF1 Antibody (C-term) flow cytometric analysis of A549 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IF (Immunofluorescence) (Confocal immunofluorescent analysis of IGF1 Antibody (C-term) with A549 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).DAPI was used to stain the cell nuclear (blue).)

WB (Western Blot) (IGF1 Antibody (C-term) western blot analysis in A549 cell line lysates (35ug/lane).This demonstrates the IGF1 antibody detected the IGF1 protein (arrow).)

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IGF1, Polyclonal Antibody (Cat# AAA289206)

Full Name

IGF1 Antibody (C-term)

Gene Names

IGF1; IGFI; IGF-I; IGF1A

Reactivity

Human

Applications

Immunohistochemistry, Flow Cytometry, Immunofluorescence, Western Blot

Purity

This antibody is purified through a protein A column, followed by peptide affinity purification.

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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WB (Western Blot) (Western blot analysis of MS4A1 (arrow) using rabbit polyclonal MS4A1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MS4A1 gene.)

WB (Western Blot) (MS4A1 Antibody (C-term) western blot analysis in HL-60 cell line lysates (35ug/lane).This demonstrates the MS4A1 antibody detected the MS4A1 protein (arrow).)

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MS4A1, Polyclonal Antibody (Cat# AAA288624)

Full Name

MS4A1 Antibody (C-term)

Gene Names

MS4A1; B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16

Reactivity

Human

Applications

Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot) (Western blot analysis of anti-PPP3CA Pab in mouse kidney tissue lysate.PPP3CA(arrow) was detected using the purified Pab.)

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Calcineurin (PPP3CA), Polyclonal Antibody (Cat# AAA288841)

Full Name

Calcineurin (PPP3CA) Antibody (N-term)

Gene Names

PPP3CA; CALN; CCN1; CNA1; CALNA; PPP2B; CALNA1

Reactivity

Human, Mouse
Predicted Reactivity: Bovine, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

WB (Western Blot) (Mouse Pnpla3 Antibody (C-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the Pnpla3 antibody detected the Pnpla3 protein (arrow).)

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Pnpla3, Polyclonal Antibody (Cat# AAA288487)

Full Name

Mouse Pnpla3 Antibody (C-term)

Gene Names

Pnpla3; Adpn

Reactivity

Mouse

Applications

Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IF (Immunofluorescence) (Fluorescent confocal image of Hela cell stained with TERT Antibody (Center). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TERT primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TERT immunoreactivity is localized to Nucleus significantly and Cytoplasm weakly.)

IF (Immunofluorescence) (Fluorescent confocal image of SY5Y cells stained with TERT (Center) antibody. SY5Y cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AAA288506 TERT (Center) primary antibody (1:200, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). TERT immunoreactivity is localized to the nucleus of SY5Y cells.)

FCM/FACS (Flow Cytometry) (TERT Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

WB (Western Blot) (Western blot analysis of anti-TERT Antibody (Center) (RB14069) in Jurkat cell line lysates (35ug/lane). TERT(arrow) was detected using the purified Pab.)

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TERT, Polyclonal Antibody (Cat# AAA288506)

Full Name

TERT Antibody (Center)

Gene Names

TERT; TP2; TRT; CMM9; EST2; TCS1; hTRT; DKCA2; DKCB4; hEST2; PFBMFT1

Reactivity

Human

Applications

Immunofluorescence, Flow Cytometry, Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot) (Western blot analysis of anti-SRPK2 Pab (AAA288175) in mouse thymus tissue lysate. SRPK2 (arrow) was detected using purified Pab. Secondary HRP-anti-rabbit was used for signal visualization with chemiluminescence.)

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SRPK2, Polyclonal Antibody (Cat# AAA288175)

Full Name

SRPK2 Antibody (C-term)

Gene Names

SRPK2; SFRSK2

Reactivity

Human, mouse

Applications

Immunohistochemistry, Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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FCM/FACS (Flow Cytometry) (SYTL2 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry) (SYTL2 Antibody (Center) IHC analysis in formalin fixed and paraffin embedded brain tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the SYTL2 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot) (Western blot analysis of SYTL2 Antibody (Center) in Hela cell line lysates (35ug/lane). SYTL2 (arrow) was detected using the purified Pab.)

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SYTL2, Polyclonal Antibody (Cat# AAA288221)

Full Name

SYTL2 Antibody (Center)

Gene Names

SYTL2; EXO4; SLP2; SLP2A; SGA72M; CHR11SYT; PPP1R151

Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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FCM/FACS (Flow Cytometry) (VILL Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IF (Immunofluorescence) (Confocal immunofluorescent analysis of VILL Antibody (Center) with WiDr cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

IHC (Immunohiostchemistry) (VILL Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human skeletal muscle followed by peroxidase conjugation of the secondary antibody and DAB staining.This data demonstrates the use of VILL Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot) (VILL Antibody (Center) western blot analysis in K562 cell line lysates (35ug/lane).This demonstrates the VILL antibody detected the VILL protein (arrow).)

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VILL, Polyclonal Antibody (Cat# AAA288335)

Full Name

VILL Antibody (Center)

Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot) (Western blot analysis of anti-URKL1 Pab in 293 cell line lysate (35ug/lane). URKL1(arrow) was detected using the purified Pab.)

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URKL1, Polyclonal Antibody (Cat# AAA288357)

Full Name

URKL1 Antibody (N-term)

Gene Names

UCKL1; UCK1L; URKL1

Reactivity

Human (Predicted Reactivity: Mouse)

Applications

Immunohistochemistry, Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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FCM/FACS (Flow Cytometry) (XRCC5 Antibody (Center K439) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

IF (Immunofluorescence) (Confocal immunofluorescent analysis of XRCC5 Antibody (Center K439) with hela cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red). DAPI was used to stain the cell nuclear (blue).)

WB (Western Blot) (XRCC5 Antibody (CenterK439) western blot analysis in human placenta tissue lysates (35ug/lane).This demonstrates the XRCC5 antibody detected the XRCC5 protein (arrow).)

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XRCC5, Polyclonal Antibody (Cat# AAA288089)

Full Name

XRCC5 Antibody (Center K439)

Gene Names

XRCC5; KU80; KUB2; Ku86; NFIV; KARP1; KARP-1

Reactivity

Human

Applications

Flow Cytometry, Western Blot, Immunofluorescence

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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FCM/FACS (Flow Cytometry) (Flow cytometric analysis of K562 cells using MDH2 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human lung carcinoma reacted with MDH2 Antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

WB (Western Blot) (MDH2 Antibody (Center) western blot analysis in A431,Hela and mouse NIH/3T3 cell line lysates (35ug/lane).This demonstrates the MDH2 antibody detected the MDH2 protein (arrow).)

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MDH2, Polyclonal Antibody (Cat# AAA289831)

Full Name

MDH2 Antibody (Center)

Gene Names

MDH2; MDH; MOR1; M-MDH; MGC:3559

Reactivity

Human, mouse (Predicted Reactivity: Yeast, Bovine, C Elegans, Monkey, Pig, Rat)

Applications

Western Blot, Flow Cytometry, Immunohistochemistry

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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FCM/FACS (Flow Cytometry) (Calponin-3 Antibody (N-term) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry) (Calponin-3 Antibody (N-term) IHC analysis in formalin fixed and paraffin embedded human skeletal muscle tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the Calponin-3 Antibody (N-term) for immunohistochemistry. Clinical relevance has not been evaluated.)

WB (Western Blot) (Western blot analysis of Calponin-3 Antibody (N-term) in A2058 cell line lysates (35ug/lane). Calponin-3 (arrow) was detected using the purified Pab.)

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Calponin-3, Polyclonal Antibody (Cat# AAA289871)

Full Name

Calponin-3 Antibody (N-term)

Reactivity

Human (Predicted Reactivity: Bovine)

Applications

Flow Cytometry, Immunohistochemistry, Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

WB (Western Blot) (OR2B6 Antibody (C-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the OR2B6 antibody detected the OR2B6 protein (arrow).)

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OR2B6, Polyclonal Antibody (Cat# AAA289901)

Full Name

OR2B6 Antibody (C-term)

Gene Names

OR2B6; OR2B1; OR2B5; OR2B1P; OR2B6P; OR5-40; OR5-41; OR6-31; dJ408B20.2

Reactivity

Human

Applications

Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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WB (Western Blot) (Western blot analysis of MARK4 (arrow) using rabbit polyclonal hMARK4-R626 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MARK4 gene (Lane 2) (Origene Technologies).)

WB (Western Blot) (Western blot analysis of anti-MARK4 Pab in mouse liver tissue lysate (35ug/lane). MARK4(arrow) was detected using the purified Pab.)

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MARK4, Polyclonal Antibody (Cat# AAA289933)

Full Name

MARK4 Antibody (C-term)

Gene Names

MARK4; MARK4L; MARK4S; MARKL1; PAR-1D; MARKL1L

Reactivity

Human

Applications

Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of RBBP8 in rat spllen tissue with RBBP8 antibody at 5 ug/mL.)

WB (Western Blot) (Western blot analysis of RBBP8 in mouse spleen tissue lysate with RBBP8 antibody at (A) 1 and (B) 2 ug/mL.)

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RBBP8, Polyclonal Antibody (Cat# AAA306732)

Full Name

RBBP8 Antibody

Gene Names

RBBP8; RIM; COM1; CTIP; JWDS; SAE2; SCKL2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity chromatography purified via peptide column

Pricing

$610/0.1 mL $1,180/2x0.1 mL $1,725/3x0.1 mL $2,235/4x0.1 mL $2,525/5x0.1 mL

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IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded Human breast cancer tissue using at dilution 1/35.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded Human liver cancer tissue using at dilution 1/35.)

Application Data (Gel: 12%SDS-PAGE Lysate: 40ug Hela cellPrimary antibody: 1/500 dilution Secondary antibody dilution: 1/8000Exposure time: 5seconds)

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STX8, Polyclonal Antibody (Cat# AAA307644)

Full Name

STX8 Antibody

Gene Names

STX8; CARB

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antigen affinity purification.

Pricing

$340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human pancreas tissue using Raf1 (Phospho-Tyr341) antibody (left)or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from Jurkat cells treated with Paclitaxel using Raf1 (Phospho-Tyr341) Antibody.The lane on the right is treated with the antigen-specific peptide.)

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C-RAF, Polyclonal Antibody (Cat# AAA308330)

Full Name

C-RAF (Phospho-Tyr341) Antibody

Gene Names

RAF1; NS5; CRAF; Raf-1; c-Raf; CMD1NN

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HeLa cells, treated with TSA 400nM 24h, using Histone H3 Antibody. The lane on the right is blocked with the synthesized peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using Histone H3 Antibody. The picture on the right is blocked with the synthesized peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using Histone H3 Antibody. The picture on the right is blocked with the synthesized peptide.)

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Histone H3, Polyclonal Antibody (Cat# AAA318010)

Full Name

Histone H3 Antibody

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from COS7 cells, treated with TSA 400nM 24h, using p53 Antibody. The lane on the right is blocked with the synthesized peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human lung carcinoma tissue, using p53 Antibody. The picture on the right is blocked with the synthesized peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using p53 Antibody. The picture on the right is blocked with the synthesized peptide.)

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p53, Polyclonal Antibody (Cat# AAA318013)

Full Name

p53 Antibody

Gene Names

TP53; P53; BCC7; LFS1; TRP53

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from COLO cells, using EMR2 Antibody. The lane on the right is blocked with the synthesized peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of COS7 cells, using EMR2 Antibody. The picture on the right is blocked with the synthesized peptide.)

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EMR2, Polyclonal Antibody (Cat# AAA318042)

Full Name

EMR2 Antibody

Gene Names

ADGRE2; VBU; EMR2; CD312

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue, using FZD5 Antibody. The picture on the right is blocked with the synthesized peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of COS7 cells, using FZD5 Antibody. The picture on the right is blocked with the synthesized peptide.)

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FZD5, Polyclonal Antibody (Cat# AAA318050)

Full Name

FZD5 Antibody

Gene Names

FZD5; HFZ5; C2orf31

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from COS7 cells treated with TNF 20ng/ml 5', using GATA2 (Phospho-Ser401) Antibody.)

ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using GATA2 (Phospho-Ser401) Antibody)

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GATA2, Polyclonal Antibody (Cat# AAA318319)

Full Name

GATA2 (Phospho-Ser401) Antibody

Gene Names

GATA2; DCML; IMD21; NFE1B; MONOMAC

Reactivity

Human

Applications

Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$345/0.05 mL $450/0.1 mL $880/2x0.1 mL $1,285/3x0.1 mL $1,660/4x0.1 mL $1,775/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from Jurkat cells, using OR3A2 Antibody. The lane on the right is blocked with the synthesized peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of MCF7 cells, using OR3A2 Antibody. The picture on the right is blocked with the synthesized peptide.)

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OR3A2, Polyclonal Antibody (Cat# AAA318186)

Full Name

OR3A2 Antibody

Gene Names

OR3A2; OR228; OLFRA04; OR17-14; OR17-228

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from Jurkat, COLO, and MCF-7 cells, using OR5AR1 Antibody. The lane on the right is blocked with the synthesized peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells, using OR5AR1 Antibody. The picture on the right is blocked with the synthesized peptide.)

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OR5AR1, Polyclonal Antibody (Cat# AAA318208)

Full Name

OR5AR1 Antibody

Gene Names

OR5AR1; OR11-209

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 30', using IRF-3 (Phospho-Ser396) Antibody.)

ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using IRF-3 (Phospho-Ser396) Antibody)

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IRF-3, Polyclonal Antibody (Cat# AAA318333)

Full Name

IRF-3 (Phospho-Ser396) Antibody

Gene Names

IRF3; IIAE7

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$345/0.05 mL $450/0.1 mL $880/2x0.1 mL $1,285/3x0.1 mL $1,660/4x0.1 mL $1,775/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from NIH/3T3 cells treated with PDGF 50ng/ml 20', using MEK1 (Phospho-Ser298) Antibody.)

ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using MEK1 (Phospho-Ser298) Antibody)

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MEK1, Polyclonal Antibody (Cat# AAA318356)

Full Name

MEK1 (Phospho-Ser298) Antibody

Gene Names

MAP2K1; CFC3; MEK1; MKK1; MAPKK1; PRKMK1

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$345/0.05 mL $450/0.1 mL $880/2x0.1 mL $1,285/3x0.1 mL $1,660/4x0.1 mL $1,775/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from Jurkat cells, using GPRC5D Antibody. The lane on the right is blocked with the synthesized peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of MCF7 cells, using GPRC5D Antibody. The picture on the right is blocked with the synthesized peptide.)

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GPRC5D, Polyclonal Antibody (Cat# AAA318109)

Full Name

GPRC5D Antibody

Reactivity

Human, Mouse

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HT-29, K562, and COS7 cells, using GPR34 Antibody. The lane on the right is blocked with the synthesized peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of LOVO cells, using GPR34 Antibody. The picture on the right is blocked with the synthesized peptide.)

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GPR34, Polyclonal Antibody (Cat# AAA318116)

Full Name

GPR34 Antibody

Gene Names

GPR34; LYPSR1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue, using GRM2 Antibody. The picture on the right is blocked with the synthesized peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of LOVO cells, using GRM2 Antibody. The picture on the right is blocked with the synthesized peptide.)

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GRM2, Polyclonal Antibody (Cat# AAA318122)

Full Name

GRM2 Antibody

Gene Names

GRM2; GLUR2; mGlu2; GPRC1B; MGLUR2

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HT-29 cells, using OR2J2 Antibody. The lane on the right is blocked with the synthesized peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells, using OR2J2 Antibody. The picture on the right is blocked with the synthesized peptide.)

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OR2J2, Polyclonal Antibody (Cat# AAA318177)

Full Name

OR2J2 Antibody

Gene Names

OR2J2; OR6-8; OR6-19; ORL684; OR6.3.8; hs6M1-6; dJ80I19.4

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HT-29 cells, using OR2T1 Antibody. The lane on the right is blocked with the synthesized peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells, using OR2T1 Antibody. The picture on the right is blocked with the synthesized peptide.)

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OR2T1, Polyclonal Antibody (Cat# AAA318182)

Full Name

OR2T1 Antibody

Gene Names

OR2T1; OR1-25

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from Jurkat cells, primary antibody was diluted at 1:1000, 4 degree over night)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Tris-EDTA,pH9.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 45min).)

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RCC1, Polyclonal Antibody (Cat# AAA319482)

Full Name

RCC1 (Phospho-Ser11) Antibody

Gene Names

RCC1; CHC1; RCC1-I; SNHG3-RCC1

Reactivity

Human, Rat, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit serum by affinity-chromatography using specific immunogen.

Pricing

$300/0.05 mL $420/0.1 mL $660/0.2 mL $1,285/2x0.2 mL $1,875/3x0.2 mL $2,430/4x0.2 mL $2,735/5x0.2 mL

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IF (Immunofluorescence) (AAA321047 staining COLO205 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis on COLO205 cell lysate using MN1 Antibody, The lane on the left is treated with the antigen-specific peptide.)

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MN1, Polyclonal Antibody (Cat# AAA321047)

Full Name

MN1 Antibody

Gene Names

MN1; MGCR; MGCR1; MGCR1-PEN; dJ353E16.2

Reactivity

Human

Applications

Immunocytochemistry, Immunofluorescence, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IF (Immunofluorescence) (AAA321065 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis on 293 cell lysate using PKCB1 Antibody, The lane on the left is treated with the antigen-specific peptide.)

WB (Western Blot) (Western blot analysis of PKCB1 expression in Mouse brain lysate)

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PKCB1, Polyclonal Antibody (Cat# AAA321065)

Full Name

PKCB1 Antibody

Gene Names

ZMYND8; RACK7; PRKCBP1; PRO2893

Reactivity

Human, Mouse

Applications

Immunocytochemistry, Immunofluorescence, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohistochemisry) (AAA320858 at 1/100 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IF (Immunofluorescence) (AAA320858 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis on 293 cell lysate using MMP3 Antibody. The lane on the left is treated with the antigen-specific peptide.)

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MMP3, Polyclonal Antibody (Cat# AAA320858)

Full Name

MMP3 Antibody

Gene Names

MMP3; SL-1; STMY; STR1; CHDS6; MMP-3; STMY1

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohiostchemistry) (AAA320726 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of extracts from mouse skeletal muscle, using RYR2 Antibody. The lane on the left was treated with blocking peptide.)

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RYR2, Polyclonal Antibody (Cat# AAA320726)

Full Name

RYR2 Antibody

Gene Names

RYR2; RyR; ARVC2; ARVD2; RYR-2; VTSIP

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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<< Previous Next >>

IF (Immunofluorescence) (AAA322014 staining COLO205 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

IF (Immunofluorescence) (AAA322014 staining K-562 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

WB (Western Blot) (Western blot analysis of HSP40 expression in COLO205 and Hela cell extract)

IHC (Immunohistochemistry) (AAA322014 at 1/100 staining Human heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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HSP40, Polyclonal Antibody (Cat# AAA322014)

Full Name

HSP40 Antibody

Gene Names

DNAJB1; Hdj1; Sis1; HSPF1; Hsp40; RSPH16B

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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<< Previous Next >>

IF (Immunofluorescence) (AAA322016 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of DDR1 expression in JK cells.The lane on the left is treated with the antigen-specific peptide.)

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DDR1, Polyclonal Antibody (Cat# AAA322016)

Full Name

DDR1 Antibody

Gene Names

DDR1; CAK; DDR; NEP; HGK2; PTK3; RTK6; TRKE; CD167; EDDR1; MCK10; NTRK4; PTK3A

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of AAA322059 expression in various lysates 1 Rat brain 2 mouse brain 3 mouse lung 4 HeLa 5 HEPG2 6 293 7 Mouse spleen 8 Rat heart)

IF (Immunofluorescence) (AAA322059 staining 293 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

WB (Western Blot) (Western blot analysis of Caspase 1 expression in 293 cells.The lane on the left is treated with the antigen-specific peptide.)

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Caspase 1, Polyclonal Antibody (Cat# AAA322059)

Full Name

Caspase 1 Antibody

Gene Names

CASP1; ICE; P45; IL1BC

Reactivity

Human, Mouse

Applications

Immunocytochemistry, Immunofluorescence, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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<< Previous Next >>

IHC (Immunohistochemistry) (AAA322084 at 1/100 staining Human urothelial cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of TGFBR2 expression in rat spleen tissue.)

WB (Western Blot) (Western blot analysis of TGFBR2 expression in HeLa cells)

IHC (Immunohiostchemistry) (AAA322084 at 1/100 staining Rat skin tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab.)

WB (Western Blot) (Western blot analysis of extracts from Mouse heart, using TGFBR2 Ab. The lane on the left was treated with blocking peptide.)

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TGFBR2, Polyclonal Antibody (Cat# AAA322084)

Full Name

TGFBR2 Antibody

Gene Names

TGFBR2; AAT3; FAA3; LDS2; MFS2; RIIC; LDS1B; LDS2B; TAAD2; TBRII; TBR-ii; TGFR-2; TGFbeta-RII

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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<< Previous Next >>

IHC (Immunohistochemisry) (AAA321975 at 1/100 staining Human lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of EDG2 expression in Jurkat cell extract)

IF (Immunofluorescence) (AAA321975 staining PC-3 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

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EDG2, Polyclonal Antibody (Cat# AAA321975)

Full Name

EDG2 Antibody

Gene Names

LPAR1; EDG2; LPA1; VZG1; GPR26; edg-2; vzg-1; Gpcr26; Mrec1.3; rec.1.3

Reactivity

Human, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of Phospho-IL-10R alpha (Tyr496) Antibody expression in HuvEc cells lysates.The lane on the right is treated with the antigen-specific peptide.)

WB (Western Blot) (Western blot analysis of IL-10R alpha phosphorylation expression in HuvEc whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA321725 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

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IL-10R alpha, Polyclonal Antibody (Cat# AAA321725)

Full Name

Phospho-IL-10R alpha (Tyr496) Antibody

Gene Names

IL10RA; CD210; IL10R; CD210a; CDW210A; HIL-10R; IL-10R1

Reactivity

Human

Applications

Immunocytochemistry, Immunofluorescence, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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<< Previous Next >>

IF (Immunofluorescence) (AAA321733 staining A431 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

WB (Western Blot) (Western blot analysis of PKD1/PKC mu phosphorylation expression in HepG2 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohistochemistry) (AF3112 at 1/200 staining CRC tissue sections by IHC-P.)

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PKD1/PKC mu, Polyclonal Antibody (Cat# AAA321733)

Full Name

Phospho-PKD1/PKC mu (Tyr463) Antibody

Gene Names

PRKD1; PKD; PKCM; CHDED; PRKCM; PKC-MU

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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<< Previous Next >>

IF (Immunofluorescence) (AAA321784 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of Phospho-IKK (Ser85) Antibody expression in Anisomycin treated HepG2 cells lysates.The lane on the right is treated with the antigen-specific peptide.)

WB (Western Blot) (Western blot analysis of IKK-gamma phosphorylation expression in Anisomycin treated HepG2 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

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IKK-gamma, Polyclonal Antibody (Cat# AAA321784)

Full Name

Phospho-IKK-gamma (Ser85) Antibody

Gene Names

IKBKG; IP; IP1; IP2; FIP3; IKKG; IPD2; NEMO; FIP-3; Fip3p; IMD33; AMCBX1; IKKAP1; ZC2HC9; IKK-gamma

Reactivity

Human

Applications

Immunocytochemistry, Immunofluorescence, Western Blot

Purity

From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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<< Previous Next >>

IHC (Immunohistochemisry) (AAA322093 at 1/100 staining Human melanoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of GluR6 expression in Mouse brain extracts)

IF (Immunofluorescence) (AAA322093 staining A-431 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

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GluR6, Polyclonal Antibody (Cat# AAA322093)

Full Name

GluR6 Antibody

Gene Names

GRM6; mGlu6; CSNB1B; GPRC1F; MGLUR6

Reactivity

Human, Mouse

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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<< Previous Next >>

IHC (Immunohistochemistry) (AAA322121 at 1/100 staining Mouse spleen tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of DNMT3B expression in HeLa cell lysate)

IF (Immunofluorescence) (AAA322121 staining MCF-7 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

IF (Immunofluorescence) (AAA322121 staining HT29 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

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DNMT3B, Polyclonal Antibody (Cat# AAA322121)

Full Name

DNMT3B Antibody

Gene Names

DNMT3B; ICF; ICF1; M.HsaIIIB

Reactivity

Human, Mouse

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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<< Previous Next >>

IF (Immunofluorescence) (AAA322139 staining HT29 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of Survivin expression in Mouse lungtissue lysates, The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohiostchemistry) (AAA322139 at 1/50 dilution staining IKB epsilon in human breast carcinoma by Immunohistochemistry using paraffin-embedded tissue)

IF (Immunofluorescence) (AAA322139 staining MCF-7 cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)

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Survivin, Polyclonal Antibody (Cat# AAA322139)

Full Name

Survivin Antibody

Gene Names

BIRC5; API4; EPR-1

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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IHC (Immunohistochemisry) (AAA322147 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

WB (Western Blot) (Western blot analysis of p95/NBS1 expression in Jurkat whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)

IF (Immunofluorescence) (AAA322147 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

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p95/NBS1, Polyclonal Antibody (Cat# AAA322147)

Full Name

p95/NBS1 Antibody

Gene Names

NBN; ATV; NBS; P95; NBS1; AT-V1; AT-V2

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

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WB (Western Blot) (Western blot analysis of BMP5 expression in HUVEC cells, The lane on the left is treated with the antigen-specific peptide.)

IHC (Immunohistochemistry) (AAA321837 at 1/100 staining human liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

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BMP5, Polyclonal Antibody (Cat# AAA321837)

Full Name

BMP5 Antibody

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.

Pricing

$335/0.1 mL $390/0.2 mL $755/2x0.2 mL $1,105/3x0.2 mL $1,430/4x0.2 mL $1,525/5x0.2 mL

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.