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Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.


Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.


Viewing 7700-7750 of 98831 product results


product-image-AAA244187_WB15.png WB (Western Blot) (Positive WB detected in Recombinant proteinAll lanes: LPTD antibody at 1:2000SecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 26.3 kDaObserved band size: 27 kDa)

lptD, Polyclonal Antibody (Cat# AAA244187)

Full Name
Rabbit anti-Escherichia coli (strain K12) lptD Polyclonal antibody
Gene Names
lptD; ECK0055; imp; JW0053; ostA; yabG
Reactivity
E Coli (strain K12)
Applications
Western Blot
Purity
Protein A/G
Pricing
product-image-AAA308330_IHC13.jpg IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human pancreas tissue using Raf1 (Phospho-Tyr341) antibody (left)or the same antibody preincubated with blocking peptide (right).)

C-RAF, Polyclonal Antibody (Cat# AAA308330)

Full Name
C-RAF (Phospho-Tyr341) Antibody
Gene Names
RAF1; NS5; CRAF; Raf-1; c-Raf; CMD1NN
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
Pricing
product-image-AAA288721_IHC10.jpg IHC (Immunohistochemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

ATG4D, Polyclonal Antibody (Cat# AAA288721)

Full Name
ATG4D Antibody (C-term)
Gene Names
ATG4D; APG4D; AUTL4; APG4-D
Reactivity
Human
Applications
Immunohistochemistry, Western Blot, Immunofluorescence
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
product-image-AAA288487_WB15.jpg WB (Western Blot) (Mouse Pnpla3 Antibody (C-term) western blot analysis in mouse kidney tissue lysates (35ug/lane).This demonstrates the Pnpla3 antibody detected the Pnpla3 protein (arrow).)

Pnpla3, Polyclonal Antibody (Cat# AAA288487)

Full Name
Mouse Pnpla3 Antibody (C-term)
Gene Names
Pnpla3; Adpn
Reactivity
Mouse
Applications
Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
product-image-AAA288506_IF10.jpg IF (Immunofluorescence) (Fluorescent confocal image of Hela cell stained with TERT Antibody (Center). Hela cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.1%, 10 min), then incubated with TERT primary antibody (1:25, 1 h at 37 degree). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:400, 50 min at 37 degree).Cytoplasmic actin was counterstained with Alexa Fluor 555 (red) conjugated Phalloidin (7units/ml, 1 h at 37 degree). Nuclei were counterstained with DAPI (blue) (10 ug/ml, 10 min). TERT immunoreactivity is localized to Nucleus significantly and Cytoplasm weakly.)

TERT, Polyclonal Antibody (Cat# AAA288506)

Full Name
TERT Antibody (Center)
Gene Names
TERT; TP2; TRT; CMM9; EST2; TCS1; hTRT; DKCA2; DKCB4; hEST2; PFBMFT1
Reactivity
Human
Applications
Immunofluorescence, Flow Cytometry, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
product-image-AAA288841_IHC13.jpg IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Calcineurin (PPP3CA), Polyclonal Antibody (Cat# AAA288841)

Full Name
Calcineurin (PPP3CA) Antibody (N-term)
Gene Names
PPP3CA; CALN; CCN1; CNA1; CALNA; PPP2B; CALNA1
Reactivity
Human, Mouse
Predicted Reactivity: Bovine, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
product-image-AAA288335_FCM10.jpg FCM/FACS (Flow Cytometry) (VILL Antibody (Center) flow cytometric analysis of K562 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

VILL, Polyclonal Antibody (Cat# AAA288335)

Full Name
VILL Antibody (Center)
Reactivity
Human
Applications
Flow Cytometry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
product-image-AAA288357_IHC13.jpg IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

URKL1, Polyclonal Antibody (Cat# AAA288357)

Full Name
URKL1 Antibody (N-term)
Gene Names
UCKL1; UCK1L; URKL1
Reactivity
Human (Predicted Reactivity: Mouse)
Applications
Immunohistochemistry, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
product-image-AAA288624_WB13.jpg WB (Western Blot) (Western blot analysis of MS4A1 (arrow) using rabbit polyclonal MS4A1 Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected (Lane 2) with the MS4A1 gene.)

MS4A1, Polyclonal Antibody (Cat# AAA288624)

Full Name
MS4A1 Antibody (C-term)
Gene Names
MS4A1; B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16
Reactivity
Human
Applications
Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
product-image-AAA288175_IHC13.jpg IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

SRPK2, Polyclonal Antibody (Cat# AAA288175)

Full Name
SRPK2 Antibody (C-term)
Gene Names
SRPK2; SFRSK2
Reactivity
Human, mouse
Applications
Immunohistochemistry, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
product-image-AAA288221_FCM11.jpg FCM/FACS (Flow Cytometry) (SYTL2 Antibody (Center) flow cytometric analysis of Hela cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

SYTL2, Polyclonal Antibody (Cat# AAA288221)

Full Name
SYTL2 Antibody (Center)
Gene Names
SYTL2; EXO4; SLP2; SLP2A; SGA72M; CHR11SYT; PPP1R151
Reactivity
Human
Applications
Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
product-image-AAA288089_FCM11.jpg FCM/FACS (Flow Cytometry) (XRCC5 Antibody (Center K439) flow cytometric analysis of Jurkat cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated donkey-anti-rabbit secondary antibodies were used for the analysis.)

XRCC5, Polyclonal Antibody (Cat# AAA288089)

Full Name
XRCC5 Antibody (Center K439)
Gene Names
XRCC5; KU80; KUB2; Ku86; NFIV; KARP1; KARP-1
Reactivity
Human
Applications
Flow Cytometry, Western Blot, Immunofluorescence
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
product-image-AAA289831_FCM11.jpg FCM/FACS (Flow Cytometry) (Flow cytometric analysis of K562 cells using MDH2 Antibody (Center)(bottom histogram) compared to a negative control cell (top histogram)FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

MDH2, Polyclonal Antibody (Cat# AAA289831)

Full Name
MDH2 Antibody (Center)
Gene Names
MDH2; MDH; MOR1; M-MDH; MGC:3559
Reactivity
Human, mouse (Predicted Reactivity: Yeast, Bovine, C Elegans, Monkey, Pig, Rat)
Applications
Western Blot, Flow Cytometry, Immunohistochemistry
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
product-image-AAA289871_FCM11.jpg FCM/FACS (Flow Cytometry) (Calponin-3 Antibody (N-term) flow cytometric analysis of A2058 cells (right histogram) compared to a negative control cell (left histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

Calponin-3, Polyclonal Antibody (Cat# AAA289871)

Full Name
Calponin-3 Antibody (N-term)
Reactivity
Human (Predicted Reactivity: Bovine)
Applications
Flow Cytometry, Immunohistochemistry, Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
product-image-AAA289901_WB15.jpg WB (Western Blot) (OR2B6 Antibody (C-term) western blot analysis in MDA-MB453 cell line lysates (35ug/lane).This demonstrates the OR2B6 antibody detected the OR2B6 protein (arrow).)

OR2B6, Polyclonal Antibody (Cat# AAA289901)

Full Name
OR2B6 Antibody (C-term)
Gene Names
OR2B6; OR2B1; OR2B5; OR2B1P; OR2B6P; OR5-40; OR5-41; OR6-31; dJ408B20.2
Reactivity
Human
Applications
Western Blot
Purity
Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)
Pricing
product-image-AAA289933_WB13.jpg WB (Western Blot) (Western blot analysis of MARK4 (arrow) using rabbit polyclonal hMARK4-R626 Antibody. 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the MARK4 gene (Lane 2) (Origene Technologies).)

MARK4, Polyclonal Antibody (Cat# AAA289933)

Full Name
MARK4 Antibody (C-term)
Gene Names
MARK4; MARK4L; MARK4S; MARKL1; PAR-1D; MARKL1L
Reactivity
Human
Applications
Western Blot
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Pricing
product-image-AAA142093_IHC8.jpg IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Stomach Tissue.)

Gastrokine 1, Polyclonal Antibody (Cat# AAA142093)

Full Name
Polyclonal Antibody to Gastrokine 1 (GKN1)
Gene Names
GKN1; FOV; CA11; AMP18; BRICD1; foveolin
Reactivity
Human, Rat, Pig
Applications
Immunohistochemistry, Immunocytochemistry, Western Blot
Purity
Affinity Chromatography
Pricing
product-image-AAA143541_IHC13.jpg IHC (Immunohiostchemistry) (DAB staining on fromalin fixed paraffin- embedded Kidney tissue))

Meteorin, Polyclonal Antibody (Cat# AAA143541)

Full Name
Polyclonal Antibody to Meteorin (METRN)
Gene Names
Metrn; Hyrac; RGD1306222
Reactivity
Mouse, Rat
Applications
Immunohistochemistry, Immunocytochemistry, Western Blot
Purity
Affinity Chromatography
Pricing
product-image-AAA242893_IF11.jpg IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells, using Stefin A antibody.)

CSTA, Polyclonal Antibody (Cat# AAA242893)

Full Name
CSTA Antibody
Gene Names
CSTA; AREI; STF1; STFA
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
product-image-AAA242599_IF13.jpg IF (Immunofluorescence) (Immunofluorescence analysis of MCF-7 cells, using CA5B antibody.)

CA5B, Polyclonal Antibody (Cat# AAA242599)

Full Name
CA5B Antibody
Gene Names
CA5B; CA-VB
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
product-image-AAA242996_IF11.jpg IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells, using RAD antibody.)

RRAD, Polyclonal Antibody (Cat# AAA242996)

Full Name
RRAD Antibody
Gene Names
RRAD; RAD; RAD1; REM3
Reactivity
Human, Mouse, Rat
Applications
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Pricing
product-image-AAA323165_WB13.jpg WB (Western Blot) (Western blot analysis of extracts of mouse testis tissue, using WBP2NL antibody.)

WBP2NL, Polyclonal Antibody (Cat# AAA323165)

Full Name
WBP2NL Antibody
Gene Names
WBP2NL; PAWP; GRAMD7
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA323468_IHC13.jpg IHC (Immunohiostchemistry) (AAA323468 at 1/100 staining Human pancreas tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

IL9, Polyclonal Antibody (Cat# AAA323468)

Full Name
IL9 Antibody
Gene Names
IL9; P40; HP40; IL-9
Reactivity
Human, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA323499_IF13.jpg IF (Immunofluorescence) (AAA323499 staining A549 cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA323499) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI (blue))

REV1, Polyclonal Antibody (Cat# AAA323499)

Full Name
REV1 Antibody
Gene Names
REV1; REV1L; AIBP80
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
Pricing
product-image-AAA323529_IHC11.jpg IHC (Immunohistochemisry) (AAA323529 at 1/100 staining Mouse heart tissue by IHC-P. Thes ample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)

NRK, Polyclonal Antibody (Cat# AAA323529)

Full Name
NRK Antibody
Gene Names
NRK; NESK
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Immunofluorescence, Western Blot
Purity
Peptide affinity purification
Pricing
product-image-AAA322469_IF10.jpg IF (Immunofluorescence) (AAA322469 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Smad2, Polyclonal Antibody (Cat# AAA322469)

Full Name
Smad2 Antibody
Gene Names
SMAD2; JV18; MADH2; MADR2; JV18-1; hMAD-2; hSMAD2
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA322473_WB13.jpg WB (Western Blot) (Western blot analysis of Actin-alpha/gamma using HeLa whole cell lysates)

Actin-alpha/gamma, Polyclonal Antibody (Cat# AAA322473)

Full Name
Actin-alpha/gamma Antibody
Gene Names
ACTC1; ACTC; ASD5; CMD1R; CMH11; LVNC4
Reactivity
Human, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA322495_IF13.jpg IF (Immunofluorescence) (AAA322495 staining COS7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Casein Kinase Igamma1, Polyclonal Antibody (Cat# AAA322495)

Full Name
Casein Kinase Igamma1 Antibody
Gene Names
CSNK1G1; CK1gamma1
Reactivity
Human, Mouse, Rat, Monkey
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using Sulfo LinkTM Coupling Resin.
Pricing
product-image-AAA323430_IHC13.jpg IHC (Immunohiostchemistry) (AAA323430 at 1/100 staining Rat liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

ARL11, Polyclonal Antibody (Cat# AAA323430)

Full Name
ARL11 Antibody
Gene Names
ARL11; ARLTS1
Reactivity
Human, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA323452_IHC13.jpg IHC (Immunohiostchemistry) (AAA323452 at 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

HSPB3, Polyclonal Antibody (Cat# AAA323452)

Full Name
HSPB3 Antibody
Gene Names
HSPB3; HMN2C; DHMN2C; HSPL27
Reactivity
Human, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Peptide affinity purification
Pricing
product-image-AAA323561_IHC13.jpg IHC (Immunohiostchemistry) (AAA323561 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

GPR161, Polyclonal Antibody (Cat# AAA323561)

Full Name
GPR161 Antibody
Gene Names
GPR161; RE2
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA323570_IHC13.jpg IHC (Immunohiostchemistry) (AAA323570 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

GPR44, Polyclonal Antibody (Cat# AAA323570)

Full Name
GPR44 Antibody
Gene Names
PTGDR2; DP2; DL1R; CD294; CRTH2; GPR44
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA323596_WB13.jpg WB (Western Blot) (Western blot analysis of FZD10 expression in H1299 whole cell lysate, The lane on the left is treated with the antigen-specific peptide.)

FZD10, Polyclonal Antibody (Cat# AAA323596)

Full Name
FZD10 Antibody
Gene Names
FZD10; Fz10; FzE7; CD350; FZ-10; hFz10
Reactivity
Human, Mouse, Monkey
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA323597_WB13.jpg WB (Western Blot) (Western blot analysis of FZD2 expression in A431 whole cell lysate, The lane on the left is treated with the antigen-specific peptide.)

FZD2, Polyclonal Antibody (Cat# AAA323597)

Full Name
FZD2 Antibody
Gene Names
FZD2; Fz2; fz-2; fzE2; hFz2
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA323606_IHC13.jpg IHC (Immunohiostchemistry) (AAA323606 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

GPR39, Polyclonal Antibody (Cat# AAA323606)

Full Name
GPR39 Antibody
Reactivity
Human
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA323666_IF13.jpg IF (Immunofluorescence) (AAA323666 staining HeLa cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/200 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)

ENO1, Polyclonal Antibody (Cat# AAA323666)

Full Name
ENO1 Antibody
Gene Names
ENO1; NNE; PPH; MPB1; ENO1L1; HEL-S-17
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA323930_IF11.jpg IF (Immunofluorescence) (AAA323930 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

SSBP2, Polyclonal Antibody (Cat# AAA323930)

Full Name
SSBP2 Antibody
Gene Names
SSBP2; HSPC116; SOSS-B2
Reactivity
Human, Mouse
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Peptide affinity purification
Pricing
product-image-AAA323374_WB13.jpg WB (Western Blot) (Western blot analysis of CD97 expression in HepG2 whole cell lysate; HCT116 whole cell lysate;, The lane on the left is treated with the antigen-specific peptide.)

CD97, Polyclonal Antibody (Cat# AAA323374)

Full Name
CD97 Antibody
Gene Names
ADGRE5; CD97; TM7LN1
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA323321_WB11.jpg WB (Western Blot) (Western blot analysis of extracts from Hela, using USP9X antibody.)

USP9X, Polyclonal Antibody (Cat# AAA323321)

Full Name
USP9X Antibody
Gene Names
USP9X; FAF; FAM; DFFRX; MRX99; MRXS99F
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA327163_WB13.jpg WB (Western Blot) (Western blot analysis of Hela whole cell lysates, using ROCK2 Antibody. The lane on the left is treated with the antigen-specific peptide.)

ROCK2, Polyclonal Antibody (Cat# AAA327163)

Full Name
ROCK2 Antibody
Gene Names
ROCK2; ROCK-II
Reactivity
Human, Mouse, Rat
Purity
Peptide affinity purification
Pricing
product-image-AAA327197_IHC11.jpg IHC (Immunohistochemisry) (AAA327197 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

Raptor, Polyclonal Antibody (Cat# AAA327197)

Full Name
Raptor Antibody
Gene Names
RPTOR; KOG1; Mip1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA327199_IHC13.jpg IHC (Immunohiostchemistry) (AAA327199 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

USP7, Polyclonal Antibody (Cat# AAA327199)

Full Name
USP7 Antibody
Gene Names
USP7; TEF1; HAUSP
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA327200_WB13.jpg WB (Western Blot) (Western blot analysis of RICTOR using HUVEC whole cell lysates)

RICTOR, Polyclonal Antibody (Cat# AAA327200)

Full Name
RICTOR Antibody
Gene Names
RICTOR; PIA; AVO3; hAVO3
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA327231_WB13.jpg WB (Western Blot) (Western blot analysis of Wnt10a using COLO205 whole cell lysates)

Wnt10a, Polyclonal Antibody (Cat# AAA327231)

Full Name
Wnt10a Antibody
Gene Names
WNT10A; OODD; SSPS; STHAG4
Reactivity
Human, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA327234_IHC13.jpg IHC (Immunohiostchemistry) (AAA327234 at 1/100 staining Rat lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

NLK, Polyclonal Antibody (Cat# AAA327234)

Full Name
NLK Antibody
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA327243_WB13.jpg WB (Western Blot) (Western blot analysis of Phospho-ULK1(S556) using HUVEC whole cell lysates)

ULK1, Polyclonal Antibody (Cat# AAA327243)

Full Name
Phospho-ULK1(Ser556) Antibody
Gene Names
ULK1; ATG1; ATG1A; UNC51; hATG1; Unc51.1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
product-image-AAA327244_IHC13.jpg IHC (Immunohiostchemistry) (AAA327244 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)

ULK1, Polyclonal Antibody (Cat# AAA327244)

Full Name
ULK1 Antibody
Gene Names
ULK1; ATG1; ATG1A; UNC51; hATG1; Unc51.1
Reactivity
Human, Mouse, Rat
Applications
Immunohistochemistry, Western Blot
Purity
Peptide affinity purification
Pricing
product-image-AAA327289_IF13.jpg IF (Immunofluorescence) (AAA327289 staining 293 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)

RSK1 p90, Polyclonal Antibody (Cat# AAA327289)

Full Name
Phospho-RSK1 p90 (Ser380) Antibody
Gene Names
RPS6KA1; RSK; HU-1; RSK1; p90Rsk; MAPKAPK1A
Reactivity
Human, Mouse, Rat
Applications
Immunocytochemistry, Immunofluorescence, Western Blot
Purity
From purified rabbit serum by affinity purification via sequential chromatography on phospho-and non-phospho-peptide affinity columns.
Pricing
product-image-AAA327295_IF11.jpg IF (Immunofluorescence) (AAA327295 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)

Renin, Polyclonal Antibody (Cat# AAA327295)

Full Name
Renin Antibody
Gene Names
REN; HNFJ2
Reactivity
Human, Mouse
Applications
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
product-image-AAA327345_IHC11.jpg IHC (Immunohistochemisry) (AAA327345 at 1/100 staining Rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4 degree C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab.)

KHDRBS1, Polyclonal Antibody (Cat# AAA327345)

Full Name
KHDRBS1 Antibody
Gene Names
KHDRBS1; p62; p68; Sam68
Reactivity
Human, Mouse
Applications
Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin (Thermo Fisher Scientific)
Pricing

What are Polyclonal Antibodies?


Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).


Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.


Key Uses of Polyclonal Antibodies


  • Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.

  • Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.

  • ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.

  • Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.

  • Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.


Why Buy Polyclonal Antibodies from AAA Biotech?


1. Ideal for Various Applications


Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.


2. Rigorous Quality Control


All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.


3. Wide Assortment of Antibodies


Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.


4. Highly Purified


Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.


FAQ


1. How are polyclonal antibodies produced?


Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.


2. How do polyclonal antibodies differ from monoclonal antibodies?


Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.


3. How should I store polyclonal antibodies?


Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.


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