At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded human liver using COPS5 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded rat kidney using COPS5 antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using COPS5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
IF (Immunofluorescence) (Immunofluorescence analysis of MCF-7 cells using IL1RN antibody. Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using IL1RN antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using PARK7 antibody. Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using PARK7 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using SSTR1 antibody (AAA37147) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.)
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using PYCARD antibody.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using PYCARD antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells using ATG13 antibody. Blue: DAPI for nuclear staining.)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded rat brain using ATG13 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded rat testis using ATG13 antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using ATG13 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 10s.)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using NPHS1 antibody (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of mouse kidney, using NPHS1 antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using NR0B1 antibody. Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using NR0B1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded human stomach using CYP2E1 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using CYP2E1 antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using CYP2E1 Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 1s.)
IF (Immunofluorescence) (Immunofluorescence analysis of MCF-7 cells using CDKN3 antibody. Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of mouse liver, using CDKN3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using PPBP antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of human serum, using PPBP antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5min.)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded human esophagus using HLA-DQA1 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human prostate using HLA-DQA1 antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using HLA-DQA1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
IF (Immunofluorescence) (Immunofluorescence of LRRC26 in human prostate tissue with LRRC26 Antibody at 20 μg/mL.)
WB (Western Blot) (Western blot analysis of LRRC26 in Jurkat cell lysate with LRRC26 antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide.)
IHC (Immunohistochemisry) (Immunohistochemistry of LRRC26 in human prostate tissue with LRRC26 antibody at 2.5 μg/ml.)
IHC (Immunohiostchemistry) (Immunohistochemistry of LRRC26 in human prostate tissue with LRRC26 antibody at 2.5 μg/mL.)
WB (Western Blot) (Western blot analysis of LRRC26 in human prostate tissue lysate with LRRC26 antibody at 0.5 μg/ml in (A) the absence and (B) the presence of blocking peptide.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using CHIA antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human esophageal cancer using CHIA antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded human liver injury using CHIA antibody at dilution of 1:100 (40x lens).)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human liver cancer using CHIA antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using CHIA antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using GALC antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using GALC antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using TMPO antibody.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse brain using TMPO Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded human tonsil using TMPO Antibody at dilution of 1:100 (40x lens).)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded rat brain using TMPO Antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using TMPO antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded rat brain using DYNC1I1 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human stomach using DYNC1I1 antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using DYNC1I1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using SFRP2 antibody. Blue: DAPI for nuclear staining.)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded mouse heart using SFRP2 antibody at dilution of 1:200 (40x lens).)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded rat brain using SFRP2 antibody at dilution of 1:200 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using SFRP2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using STAM2 antibody. Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using STAM2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using DDAH2 antibody. Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using DDAH2 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using TGFB2 antibody (AAA37464) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human colon using UFD1L antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using UFD1L antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
WB (Western Blot) (Western blot analysis of extracts of Rat lung, using TBX21 antibody (AAA37496) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25?g per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit. Exposure time: 30s.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using GNPAT antibody (AAA37508) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 5s.)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded rat brain using ARHGAP5 antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using ARHGAP5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human stomach using PLS3 antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using PLS3 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using EEF1D antibody. Blue: DAPI for nuclear staining.)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded mouse brain using EEF1D antibody at dilution of 1:200 (40x lens).)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded rat brain using EEF1D antibody at dilution of 1:200 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using EEF1D antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse stomach using AP2B1 Antibody AAA37556 at dilution of 1:100 (40x lens).)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded mouse lung using AP2B1 Antibody AAA37556 at dilution of 1:100 (40x lens).)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human esophagus using AP2B1 Antibody AAA37556 at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using AP2B1 AAA37556 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
IF (Immunofluorescence) (Immunofluorescence analysis of MCF-7 cells using PDCD5 antibody.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using PDCD5 Antibody at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 10s.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using BMP15 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human stomach using BMP15 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded human kidney using BMP15 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded rat kidney using BMP15 antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using BMP15 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 30s.)
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.
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