At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded mouse brain using HIP1 antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using HIP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)
IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using RBBP5 antibody. Blue: DAPI for nuclear staining.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse liver using RBBP5 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using RBBP5 antibody at dilution of 1:100 (40x lens).)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded rat Intestine using RBBP5 antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using RBBP5 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 10s.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse liver using H2AFZ antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded human stomach using H2AFZ antibody at dilution of 1:100 (40x lens).)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using H2AFZ antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using H2AFZ antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells using MORF4L1 antibody.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using MORF4L1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human gastric cancer using GIP antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using GIP antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded human colon using GIP antibody at dilution of 1:100 (40x lens).)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded rat kidney using GIP antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using GIP antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 60s.)
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using ST3GAL4 antibody. Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using ST3GAL4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 60s.)
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using BAP1 antibody. Blue: DAPI for nuclear staining.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using BAP1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 30s.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human esophagus using CPM antibody at dilution of 1:100 (40x lens).)
IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded human liver injury using CPM antibody at dilution of 1:100 (40x lens).)
IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human stomach using CPM antibody at dilution of 1:100 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts of HeLa cells, using CPM Antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 15s.)
IF (Immunofluorescence) (Immunofluorescent analysis of TCEB3C staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of TCEB3C staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
WB (Western Blot) (Western blot analysis of TCEB3C expression in Jurkat (A), mouse kidney (B), rat kidney (C) whole cell lysates.)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of VCP staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
WB (Western Blot) (Western blot analysis of VCP expression in HeLa (A), K562 (B), Raw264.7 (C) whole cell lysates.)
WB (Western Blot) (Western blot analysis of Human, Mouse Cell line lysates showing detection of TLR4 protein using Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200). Primary Antibody: Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200) at 1:1000.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis using Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200). Tissue: colon colitis. Species: Mouse. Fixation: Formalin. Primary Antibody: Rabbit Anti-TLR4 Polyclonal Antibody (SPC-200) at 1:100000 for 12 hours at 4 degree C. Secondary Antibody: Biotin Goat Anti-Rabbit at 1:2000 for 1 hour at RT. Counterstain: Methyl Green at 200uL for 2 min at RT. Localization: Inflammatory cells.)
IHC (Immunohiostchemistry) (Anti-TNF Receptor I antibody, AAA44965, IHC(P)IHC(P): Human Mammary Tissue)
WB (Western Blot) (Anti-TNF Receptor I antibody, AAA44965, Western blottingLane 1: Recombinant Human TNFR1 Protein 10ngLane 2: Recombinant Human TNFR1 Protein 5ngLane 3: Recombinant Human TNFR1 Protein 2.5ng)
IF (Immunofluorescence) (Immunofluorescent analysis of E Cadherin staining in PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)
IHC (Immunohiostchemistry) (Immunohistochemical analysis of E Cadherin staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)
WB (Western Blot) (Western blot analysis of E Cadherin expression in HEK293T (A), PC12 (B), A431 (C), MCF7 (D), C2C12 (E) whole cell lysates.)
IHC (Immunohiostchemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human cervical cancer tissue using AAA238278(USP2 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200))
IHC (Immunohistochemistry) (The image on the left is immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using AAA238278(USP2 Antibody) at dilution 1/30, on the right is treated with fusion protein. (Original magnification: ×200))
IF (Immunofluorescence) (AAA322775 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25 degree C. Samples were then incubated with primary Ab (AAA322775 1:200) and mouse anti-beta tubulin Ab for 1 hour at 37 degree C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI(blue).)
IHC (Immunohistochemistry) (AAA322775 at 1/100 staining Mouse kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4 degree C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab.)
WB (Western Blot) (Western blot analysis of P2RY9 using HeLa whole cell lysates)
IHC (Immunohistochemistry) (AAA322801 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of extracts of HL-60 cells, using Centrin 1 antibody.)
IHC (Immunohistochemistry) (AAA322921 at 1/100 staining Human kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IF (Immunofluorescence) (AAA322930 staining Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C.Samples were then incubated with primary Ab(AAA322930 1:200) and mouse anti-beta tubulin Ab( 1:200) for 1 hour at 37°C.An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab.The nuclear counter stain is DAPI(blue).)
WB (Western Blot) (Western blot analysis of extracts of SW 1990 cells, using QSOX1 antibody.)
IHC (Immunohiostchemistry) (AAA322930 at 1/100 staining Rat ovary tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab.)
IHC (Immunohistochemistry) (AAA322930 at 1/100 staining mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of extracts of rat brain tissue, using FBXW7 antibody.)
WB (Western Blot) (Western blot analysis on HEK-293 cell lysate using FBXW7 Antibody)
IHC (Immunohistochemistry) (AAA323224 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohiostchemistry) (AAA323097 at 1/100 staining Human lymph cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of extracts of K-562 cells, using FECH antibody.)
IHC (Immunohiostchemistry) (AAA323101 at 1/100 staining Human lymph cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of extracts of K-562 cells, using Hemoglobin epsilon antibody.)
WB (Western Blot) (Western blot analysis of p130 Cas expression in NIH-3T3 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohiostchemistry) (AAA322353 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IF (Immunofluorescence) (AAA322353 staining NIH-3T3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
WB (Western Blot) (Western blot analysis of extracts of mouse embryo tissue, using TCTN1 antibody.)
IHC (Immunohistochemistry) (AAA322856 at 1/100 staining Human lymph node tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemisry) (AAA323020 at 1/100 staining Human pancreas tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western Blot analysis of extracts from rat skin tissue, using Dermatopontin antibody.)
WB (Western Blot) (Western Blot analysis of extracts from Mouse liver, using Dermatopontin Antibody. Lane 1 was treated with the blocking peptide.)
WB (Western Blot) (Western blot analysis of extracts of mouse brain tissue, using EXOC1 antibody.)
IHC (Immunohistochemistry) (AAA323025 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemisry) (AAA322323 at 1/100 staining Mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of SYK expression in UV treated K562 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
IF (Immunofluorescence) (AAA322323 staining COS7 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
IHC (Immunohistochemisry) (AAA325837 at 1/100 staining Human lung cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IF (Immunofluorescence) (AAA325837 staining Hela cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
WB (Western Blot) (Western blot analysis of extracts of mouse kidney, using CYCS antibody. The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemisry) (AAA325994 at 1/100 staining Human urothelial cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of Y79 using AKR1A antibody. The lane on the left is treated with the antigen-specific peptide.)
IF (Immunofluorescence) (AAA325994 staining COLO205 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
IHC (Immunohiostchemistry) (AAA326052 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of Hela whole cell lysates, using MCM7 Antibody. The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot) (Western blot analysis of extracts from rat muscle, mouse liver, using RALBP1 Antibody.)
WB (Western Blot) (Western blot analysis of K562 lysate using RALBP1 antibody. The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohiostchemistry) (AAA326054 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of extracts from mouse brain, using RALBP1 Antibody. Lane 1 was treated with the antigen-specific peptide.)
WB (Western Blot) (Western blot analysis of K562 lysate usingRAB7A antibody. The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot) (Western blot analysis of extracts from rat brain, using RAB7A Antibody.)
IHC (Immunohiostchemistry) (AAA326067 at 1/100 staining Mouse muscle tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of extracts from Hela, using RAB7A Antibody. Lane 1 was treated with the antigen-specific peptide.)
IHC (Immunohiostchemistry) (AAA325931 at 1/100 staining Human lymph node tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of brain lysate using CTSB antibody. The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot) (Western blot analysis of HT-29 using MTAP antibody. The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot) (Western blot analysis of extracts from Mouse brain lysate, using MTAP antibody.)
IHC (Immunohistochemistry) (AAA325978 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohiostchemistry) (AAA326154 at 1/100 staining Human liver cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of brain lysate using VAMP2 antibody. The lane on the left is treated with the antigen-specific peptide.)
IF (Immunofluorescence) (AAA326178 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
WB (Western Blot) (Western blot analysis of extracts of mouse hearttissue, using FGB antibody. The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemistry) (AAA326178 at 1/100 staining Human melanoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of Hela whole cell lysates, using RBBP4 Antibody. The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemistry) (AAA326198 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of extracts of HepG2, using PSMC5 antibody. The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemistry) (AAA326214 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IF (Immunofluorescence) (AAA326249 staining A549 by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab (AAA326249) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody. The nuclear counter stain is DAPI (blue).)
WB (Western Blot) (Western blot analysis of extracts from rat spleen, using ABO Antibody.)
IHC (Immunohiostchemistry) (AAA326249 at 1/100 staining Rat kidney tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab.)
IHC (Immunohistochemistry) (AAA326249 at 1/100 staining Human lymph node tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of extracts of mouse testis tissue, using RASA1 antibody. The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemistry) (AAA326286 at 1/100 staining Mouse placenta tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of extracts of A549, using PTHLH antibody. The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemistry) (AAA326298 at 1/100 staining Mouse colon tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of A549 whole cell lysates, using PYCARD Antibody. The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohiostchemistry) (AAA326083 at 1/100 staining Mouse lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IF (Immunofluorescence) (AAA326083 staining RAW264.7 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot
Purity
The antiserum was purified by peptide affinity chromatography using SulfoLink Coupling Resin.
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.
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