Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using RARB Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Rat kidney using RARB Polyclonal Antibody at dilution of 1:100 (40x lens).)

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RARB, Polyclonal Antibody (Cat# AAA177330)

Full Name

RARB Polyclonal Antibody

Gene Names

RARB; HAP; RRB2; NR1B2

Reactivity

Human, Rat

Applications

Immunofluorescence, Immunohistochemistry

Purity

Affinity purification

Pricing

$300/0.06 mL $410/0.12 mL $630/0.2 mL $1,220/2x0.2 mL $1,780/3x0.2 mL $2,315/4x0.2 mL $2,830/5x0.2 mL

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WB (Western Blot) (Host: RabbitTarget Name: GSPT1Sample Tissue: Human HepG2 Whole CellAntibody Dilution: 1ug/ml)

WB (Western Blot) (WB Suggested Anti-GSPT1 AntibodyTitration: 1.0 ug/mlPositive Control: HepG2 Whole CellGSPT1 is strongly supported by BioGPS gene expression data to be expressed in Human HepG2 cells)

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GSPT1, Polyclonal Antibody (Cat# AAA201309)

Full Name

GSPT1 Antibody - N-terminal region

Gene Names

GSPT1; GST1; ETF3A; eRF3a; 551G9.2

Reactivity

Tested Species Reactivity: Human
Predicted Species Reactivity: Human, Rat, Cow, Dog, Pig, Rabbit

Applications

Western Blot

Purity

Affinity Purified

Pricing

$500/0.1 mL $975/2x0.1 mL $1,430/3x0.1 mL $1,855/4x0.1 mL $2,115/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of Mouse spleen tissue using PI 3-kinase p85? Polyclonal Antibody at dilution of 1:200.)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human uterus tissue using PI 3-kinase p85? Polyclonal Antibody at dilution of 1:200.)

WB (Western Blot) (Western Blot analysis of various cells using PI 3-kinase p85? Polyclonal Antibody at dilution of 1:2000.)

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PI 3-kinase p85alpha, Polyclonal Antibody (Cat# AAA171686)

Full Name

PI 3-kinase p85alpha Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human lung tissue using Integrin ?D Polyclonal Antibody at dilution of 1:100.)

WB (Western Blot) (Western Blot analysis of 4T1, 293T cells with Integrin ?D Polyclonal Antibody.)

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Integrin alphaD, Polyclonal Antibody (Cat# AAA171880)

Full Name

Integrin alphaD Polyclonal Antibody

Reactivity

Human

Applications

Western Blot

Purity

Antigen Affinity Purified

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using ADORA2B Polyclonal Antibody at dilution of 1:30(×200))

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using ADORA2B Polyclonal Antibody at dilution of 1:30(×200))

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ADORA2B, Polyclonal Antibody (Cat# AAA175709)

Full Name

ADORA2B Polyclonal Antibody

Gene Names

ADORA2B; ADORA2

Reactivity

Human

Applications

Immunohistochemistry

Purity

Antigen Affinity Purification

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

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IHC (Immunohistochemisry) (Immunohistochemistry analysis of paraffin-embedded rat brain using GAP43 Polyclonal Antibody at dilution of 1:300.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded mouse brain using GAP43 Polyclonal Antibody at dilution of 1:300.)

WB (Western Blot) (Western Blot analysis of various samples using GAP43 Polyclonal Antibody at dilution of 1:2000.)

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GAP43, Polyclonal Antibody (Cat# AAA174372)

Full Name

GAP43 Polyclonal Antibody

Gene Names

GAP43; B-50; PP46

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$255/0.06 mL $355/0.12 mL $505/0.2 mL $985/2x0.2 mL $1,440/3x0.2 mL $1,875/4x0.2 mL $2,255/5x0.2 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using BAG3 Polyclonal Antibody at dilution of 1:35(×200))

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human cervical cancer tissue using BAG3 Polyclonal Antibody at dilution of 1:35(×200))

WB (Western Blot) (Western blot analysis of Mouse heart tissue lysate using BAG3 Polyclonal Antibody at dilution of 1:250)

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BAG3, Polyclonal Antibody (Cat# AAA175045)

Full Name

BAG3 Polyclonal Antibody

Gene Names

BAG3; BIS; MFM6; BAG-3; CAIR-1

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purification

Pricing

$180/0.02 mL $245/0.06 mL $350/0.12 mL $505/0.2 mL $985/2x0.2 mL $1,440/3x0.2 mL $1,875/4x0.2 mL $2,240/5x0.2 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human liver cancer tissue using TMED10 Polyclonal Antibody at dilution of 1:65(×200))

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human lung cancer tissue using TMED10 Polyclonal Antibody at dilution of 1:65(×200))

WB (Western Blot) (Western blot analysis of NIH/3T3 cell Mouse Pancreas tissue Rat lung tissue Mouse liver tissue Hela Raji cell lysates using TMED10 Polyclonal Antibody at dilution of 1:900)

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TMED10, Polyclonal Antibody (Cat# AAA175608)

Full Name

TMED10 Polyclonal Antibody

Gene Names

TMED10; p23; TMP21; p24d1; S31I125; Tmp-21-I; S31III125; P24(DELTA)

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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WB (Western Blot) (Western blot analysis of extracts from wild type(WT) and HER2/ErbB2 knockout (KO) HeLa cells using HER2/ErbB2 Polyclonal Antibody at 1:500 dilution.)

WB (Western Blot) (Western blot analysis of MCF7 using HER2/ErbB2 Polyclonal Antibody at 1:500 dilution.)

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HER2/ErbB2, Polyclonal Antibody (Cat# AAA179592)

Full Name

(KO Validated) HER2/ErbB2 Polyclonal Antibody

Gene Names

ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu

Reactivity

Human

Applications

Western Blot

Purity

Affinity purification

Pricing

$315/0.06 mL $450/0.12 mL $685/0.2 mL $1,335/2x0.2 mL $1,945/3x0.2 mL $2,525/4x0.2 mL $3,075/5x0.2 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded rat brain using SHANK3 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human brain using SHANK3 Polyclonal Antibody at dilution of 1:100 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of extracts of Mouse brain using SHANK3 Polyclonal Antibody at 1:1000 dilution.)

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SHANK3, Polyclonal Antibody (Cat# AAA179286)

Full Name

SHANK3 Polyclonal Antibody

Gene Names

SHANK3; PSAP2; SCZD15; PROSAP2; SPANK-2; DEL22q13.3

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity purification

Pricing

$305/0.06 mL $420/0.12 mL $650/0.2 mL $1,260/2x0.2 mL $1,845/3x0.2 mL $2,390/4x0.2 mL $2,880/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using BRPF1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of L929 cells using BRPF1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using BRPF1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of Mouse testis using BRPF1 Polyclonal Antibody at1:1000 dilution.)

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BRPF1, Polyclonal Antibody (Cat# AAA179084)

Full Name

BRPF1 Polyclonal Antibody

Gene Names

BRPF1; BR140

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Affinity purification

Pricing

$300/0.06 mL $410/0.12 mL $630/0.2 mL $1,220/2x0.2 mL $1,780/3x0.2 mL $2,315/4x0.2 mL $2,830/5x0.2 mL

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WB (Western Blot) (Western blot of VISA in rat brain tissue lysate with VISA antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.)

IF (Immunofluorescence) (Immunofluorescence of VISA in Mouse Brain cells with VISA antibody at 20 ug/ml.)

IHC (Immunohistochemistry) (Anti-MAVS antibody IHC of human brain, cortex. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

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VISA / MAVS, Polyclonal Antibody (Cat# AAA50805)

Full Name

Rabbit Polyclonal to Human VISA / MAVS

Gene Names

MAVS; IPS1; VISA; IPS-1; CARDIF

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Immunoaffinity Purified

Pricing

$665/0.05 mg $1,295/2x0.05 mg $1,890/3x0.05 mg $2,450/4x0.05 mg $2,775/5x0.05 mg

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WB (Western Blot) (Staining (0.5ug/ml) of Daudi (A), Jurkat (B) and K562 (C) lysates (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

WB (Western Blot) (Staining (0.3ug/ml) of MOLT4 lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

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LIVIN/BIRC7, Polyclonal Antibody (Cat# AAA60853)

Full Name

Goat anti-LIVIN/BIRC7 Antibody

Gene Names

BIRC7; KIAP; LIVIN; MLIAP; RNF50; ML-IAP

Reactivity

Tested: Human; Expected from sequence similarity: Human

Applications

Western Blot, Peptide ELISA

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

$460/0.1 mg $900/2x0.1 mg $1,310/3x0.1 mg $1,700/4x0.1 mg $1,880/5x0.1 mg

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FCM/FACS (Flow Cytometry) (Figure 3. Flow Cytometry analysis of THP-1 cells using anti-Azurocidin/AZU1 antibody (AAA126416).Overlay histogram showing THP-1 cells stained with AAA126416 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Azurocidin/AZU1 Antibody (AAA126416, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohiostchemistry) (Figure 2. IHC analysis of Azurocidin/AZU1 using anti-Azurocidin/AZU1 antibody (AAA126416).Azurocidin/AZU1 was detected in a paraffin-embedded section of human appendicitis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Azurocidin/AZU1 Antibody (AAA126416) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of Azurocidin/AZU1 using anti-Azurocidin/AZU1 antibody (AAA126416).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: rat spleen tissue lysates,Lane 2: rat thymus tissue lysates,Lane 3: mouse spleen tissue lysates,Lane 4: mouse thymus tissue lysates,Lane 5: mouse RAW264.7 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Azurocidin/AZU1 antigen affinity purified polyclonal antibody (#AAA126416) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Azurocidin/AZU1 at approximately 37 kDa. The expected band size for Azurocidin/AZU1 is at 37 kDa.)

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Azurocidin/AZU1, Polyclonal Antibody (Cat# AAA126416)

Full Name

Anti-Azurocidin/AZU1 Antibody Picoband

Gene Names

AZU1; AZU; HBP; NAZC; AZAMP; CAP37; HUMAZUR

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $705/0.1 mg (Dylight594) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $810/0.1 mg (APC) $2,005/5x0.1 mg

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FCM/FACS (Flow Cytometry) (Figure 4. Flow Cytometry analysis of HL-60 cells using anti-TOX antibody (AAA126522).Overlay histogram showing HL-60 cells stained with AAA126522 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-TOX Antibody (AAA126522, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemisry) (Figure 3. IHC analysis of TOX using anti-TOX antibody (AAA126522).TOX was detected in a paraffin-embedded section of mouse lymphaden tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-TOX Antibody (AAA126522) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohiostchemistry) (Figure 2. IHC analysis of TOX using anti-TOX antibody (AAA126522).TOX was detected in a paraffin-embedded section of human intestinal cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-TOX Antibody (AAA126522) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of TOX using anti-TOX antibody (AAA126522).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Jurkat whole cell lysates,Lane 2: human Ramos whole cell lysates,Lane 3: human HL-60 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-TOX antigen affinity purified polyclonal antibody (#AAA126522) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for TOX at approximately 57 kDa. The expected band size for TOX is at 57 kDa.)

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TOX, Polyclonal Antibody (Cat# AAA126522)

Full Name

Anti-TOX Antibody Picoband

Gene Names

TOX; TOX1

Reactivity

Human, Mouse

Applications

Flow Cytometry, Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

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FCM/FACS (Flow Cytometry) (Figure 5. Flow Cytometry analysis of SiHa cells using anti-FADS1 antibody (AAA126233).Overlay histogram showing SiHa cells stained with AAA126233 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FADS1 Antibody (AAA126233, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of FADS1 using anti-FADS1 antibody (AAA126233).FADS1 was detected in a paraffin-embedded section of human ovarian cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-FADS1 Antibody (AAA126233) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemisry) (Figure 3. IHC analysis of FADS1 using anti-FADS1 antibody (AAA126233).FADS1 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-FADS1 Antibody (AAA126233) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohiostchemistry) (Figure 2. IHC analysis of FADS1 using anti-FADS1 antibody (AAA126233).FADS1 was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-FADS1 Antibody (AAA126233) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of FADS1 using anti-FADS1 antibody (AAA126233).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human HepG2 whole cell lysates,Lane 2: human A549 whole cell lysates,Lane 3: human Jurkat whole cell lysates,Lane 4: human Hela whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-FADS1 antigen affinity purified polyclonal antibody (#AAA126233) at 0.25 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for FADS1 at approximately 52 kDa. The expected band size for FADS1 is at 52 kDa.)

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FADS1, Polyclonal Antibody (Cat# AAA126233)

Full Name

Anti-FADS1 Antibody Picoband

Gene Names

FADS1; D5D; TU12; FADS6; FADSD5; LLCDL1

Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

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FCM/FACS (Flow Cytometry) (Figure 3. Flow Cytometry analysis of MCF-7 cells using anti-PI4K2B antibody (AAA127692).Overlay histogram showing MCF-7 cells stained with AAA127692 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PI4K2B Antibody (AAA127692, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 2. IF analysis of PI4K2B using anti-PI4K2B antibody (AAA127692).PI4K2B was detected in an immunocytochemical section of A549 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-PI4K2B Antibody (AAA127692) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The tissue section was developed using Phalloidin-iFluor 488 Conjugated. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

WB (Western Blot) (Figure 1. Western blot analysis of PI4K2B using anti-PI4K2B antibody (AAA127692).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human HepG2 whole cell lysates,Lane 2: human 293T whole cell lysates,Lane 3: human Hela whole cell lysates,Lane 4: human MCF-7 whole cell lysates,Lane 5: rat liver tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PI4K2B antigen affinity purified polyclonal antibody (#AAA127692) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PI4K2B at approximately 50 kDa. The expected band size for PI4K2B is at 55,50 kDa.)

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PI4K2B, Polyclonal Antibody (Cat# AAA127692)

Full Name

Anti-PI4K2B Antibody Picoband

Gene Names

PI4K2B; PIK42B; PI4KIIB

Reactivity

Human, Rat

Applications

Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $705/0.1 mg (Dylight594) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $810/0.1 mg (APC) $2,005/5x0.1 mg

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FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of Raji cells using anti-Nir2/PITPNM1 antibody (AAA127503).Overlay histogram showing Raji cells stained with AAA127503 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Nir2/PITPNM1 Antibody (AAA127503, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

WB (Western Blot) (Figure 1. Western blot analysis of Nir2/PITPNM1 using anti-Nir2/PITPNM1 antibody (AAA127503).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: rat brain tissue lysates,Lane 2: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Nir2/PITPNM1 antigen affinity purified polyclonal antibody (#AAA127503) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Nir2/PITPNM1 at approximately 150 kDa. The expected band size for Nir2/PITPNM1 is at 135 kDa.)

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Nir2/PITPNM1, Polyclonal Antibody (Cat# AAA127503)

Full Name

Anti-Nir2/PITPNM1 Antibody Picoband

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

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FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of RT4 cells using anti-PTGR1 antibody (AAA127533).Overlay histogram showing RT4 cells stained with AAA127533 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PTGR1 Antibody (AAA127533, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

WB (Western Blot) (Figure 1. Western blot analysis of PTGR1 using anti-PTGR1 antibody (AAA127533).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human HepG2 whole cell lysates,Lane 3: human A549 whole cell lysates,Lane 4: human RT4 whole cell lysates,Lane 5: human SiHa whole cell lysates,Lane 6: human Hacat whole cell lysates,Lane 7: human Caco-2 whole cell lysates,Lane 8: human PC-3 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PTGR1 antigen affinity purified polyclonal antibody (#AAA127533) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PTGR1 at approximately 36 kDa. The expected band size for PTGR1 is at 36 kDa.)

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PTGR1, Polyclonal Antibody (Cat# AAA127533)

Full Name

Anti-PTGR1 Antibody Picoband

Gene Names

PTGR1; PGR1; ZADH3; LTB4DH

Reactivity

Human

Applications

Flow Cytometry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg (Carrier Free) $485/0.1 mg $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (Dylight594) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (PE) $810/0.1 mg (iFluor647) $2,005/5x0.1 mg

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FCM/FACS (Flow Cytometry) (Figure 4. Flow Cytometry analysis of h. PBMC cells using anti-CCR2 antibody (AAA125520).Overlay histogram showing h. PBMC cells stained with AAA125520 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CCR2 Antibody (AAA125520, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemisry) (Figure 3. IHC analysis of CCR2 using anti-CCR2 antibody (AAA125520).CCR2 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CCR2 Antibody (AAA125520) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohiostchemistry) (Figure 2. IHC analysis of CCR2 using anti-CCR2 antibody (AAA125520).CCR2 was detected in paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH8. 0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2μg/ml rabbit anti-CCR2 Antibody (AAA125520) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of CCR2 using anti-CCR2 antibody (AAA125520).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HL-60 whole cell lysates,After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-CCR2 antigen affinity purified polyclonal antibody (Catalog # AAA125520) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CCR2 at approximately 42KD. The expected band size for CCR2 is at 42KD.)

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CCR2, Polyclonal Antibody (Cat# AAA125520)

Full Name

Anti-CCR2 Antibody

Gene Names

CCR2; CKR2; CCR-2; CCR2A; CCR2B; CD192; CKR2A; CKR2B; CMKBR2; MCP-1-R; CC-CKR-2

Reactivity

Human

Applications

Direct ELISA, Flow Cytometry, Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (PE) $810/0.1 mg (iFluor647) $2,005/5x0.1 mg

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FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of U87 cells using anti-Epac1/RAPGEF3 antibody (AAA125656).Overlay histogram showing U87 cells stained with AAA125656 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Epac1/RAPGEF3 Antibody (AAA125656, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

WB (Western Blot) (Figure 1. Western blot analysis of Epac1/RAPGEF3 using anti-Epac1/RAPGEF3 antibody (AAA125656).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human HELA whole cell lysatesLane 2: human placenta tissue lysatesLane 3: monkey kidney tissue lysatesLane 4: rat kidney tissue lysatesLane 5: rat lung tissue lysatesLane 6: mouse kidney tissue lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-Epac1/RAPGEF3 antigen affinity purified polyclonal antibody (Catalog # AAA125656) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Epac1/RAPGEF3 at approximately 104KD. The expected band size for Epac1/RAPGEF3 is at 104KD.)

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Epac1/RAPGEF3, Polyclonal Antibody (Cat# AAA125656)

Full Name

Anti-Epac1/RAPGEF3 Antibody

Gene Names

RAPGEF3; EPAC; EPAC1; bcm910; HSU79275; CAMP-GEFI

Reactivity

Human, Mouse, Rat, Monkey

Applications

Direct ELISA, Flow Cytometry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

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WB (Western Blot) ((1ug/ml) staining of Mouse Brain lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

WB (Western Blot) ((0.5ug/ml) staining of Human Frontal Cortex lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

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OX1R and OX2R, Polyclonal Antibody (Cat# AAA60913)

Full Name

Goat anti-OX1R and OX2R Antibody

Gene Names

HCRTR1; OX1R

Reactivity

Tested: Human, Mouse, Rat; Expected from sequence similarity: Human, Mouse, Rat, Dog, Pig, Cow

Applications

Western Blot, Peptide ELISA

Purity

Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.

Pricing

$460/0.1 mg $900/2x0.1 mg $1,310/3x0.1 mg $1,700/4x0.1 mg $1,880/5x0.1 mg

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WB (Western Blot) (Western blot of anti-ACE2 (SARS Receptor) Antibody (Center) in HL60 cell line lysates (35 ug/lane). ACE2 (arrow) was detected using the purified Pab.)

FCM/FACS (Flow Cytometry) (Flow cytometry of HL-60 cells using ACE2 (SARS Receptor) Antibody (Center) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohistochemisry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

IHC (Immunohiostchemistry) (Anti-ACE-2 antibody IHC of human testis. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

IHC (Immunohistochemistry) (Anti-ACE-2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

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ACE2 / ACE-2, Polyclonal Antibody (Cat# AAA51252)

Full Name

Rabbit Polyclonal to Human ACE2 / ACE-2

Gene Names

ACE2; ACEH

Reactivity

Human

Applications

Flow Cytometry, Western Blot, Immunohistochemistry

Purity

Ammonium sulfate precipitation

Pricing

$665/0.2 mL $1,295/2x0.2 mL $1,890/3x0.2 mL $2,450/4x0.2 mL $2,775/5x0.2 mL

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DB (Dot Blot) (Histone H3K27ac antibody (pAb) tested by dot blot analysis. Dot blot analysis was used to confirm the specificity of Histone H3K27ac antibody (pAb) for acetyl Lys27 histone H3. Acetylated peptides corresponding to the immunogen and related peptides were spotted onto PVDF and probed with the antibody at a dilution of 1:1,000. The amount of peptide (picomoles) spotted is indicated next to each row. Lane 1: H3K37ac. Lane 2: H3K36ac. Lane 3: H3K9ac. Lane 4: H3K14ac. Lane 5: H3K18ac. Lane 6: H3K23ac. Lane 7: unmod H3K27. Lane 8: H3K27ac. Lane 9: H4K5ac. Lane 10: H4K8ac. Lane 11: H4K12ac. Lane 12: H4K16ac.)

WB (Western Blot) (Histone H3K27ac antibody (pAb) tested by Western blot. HeLa acid extract probed with Histone H3 acetyl Lys27 polyclonal antibody (1:1,000 dilution). Lane 1: No treatment. Lane 2: Cells treated with sodium butyrate.)

ChIP (Chromatin Immunoprecipitation) (Histone H3K27ac antibody (pAb) tested by ChIP Chromatin IP performed using the ChIP-IT Express Kit and HeLa Chromatin (2.1 x 106 cell equivalents per ChIP) using 3 ul of Histone H3 acetyl Lys27 antibody or the equivalent amount of rabbit IgG as a negative control. Real time, quantitative PCR (RT-qPCR) was performed on DNA purified from each of the ChIP reactions using primer pairs specific for the indicated gene as well as a negative control primer pair. Data are presented as Fold Enrichment of the ChIP antibody signal versus the negative control IgG using the ddCT method.)

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Histone H3K27ac, Polyclonal Antibody (Cat# AAA59837)

Full Name

Histone H3K27ac antibody (pAb)

Reactivity

Budding Yeast, Human, Wide Range Predicted

Applications

Western Blot, Dot Blot, Chromatin immunoprecipitation, Chromatin immunoprecipitation

Pricing

$250/0.01 mL $455/0.05 mL $725/0.1 mL $1,415/2x0.1 mL $2,070/3x0.1 mL $2,680/4x0.1 mL $3,100/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence of IL-31 in Human Skeletal Muscle cells with IL-31 antibody at 20 μg/mL.)

WB (Western Blot) (Western blot analysis of IL-31 in human skeletal muscle tissue lysate with IL-31 antibody at (A) 2.5, (B) 5 and (C) 10 μg/mL.)

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IL-31, Polyclonal Antibody (Cat# AAA39869)

Full Name

IL-31 Antibody

Gene Names

Il31; 1700013B14Rik

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

IL-31 Antibody is affinity chromatography purified via peptide column.

Pricing

$545/0.1 mg $1,050/2x0.1 mg $1,530/3x0.1 mg $1,985/4x0.1 mg $2,270/5x0.1 mg

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IF (Immunofluorescence) (Immunofluorescence of LIMP2 in Human Skeletal Muscle tissue with LIMP2 antibody at 20 μg/mL.)

IHC (Immunohiostchemistry) (Immunohistochemistry of LIMP2 in human skeletal muscle tissue with LIMP2 antibody at 2.5 μg/mL.)

WB (Western Blot) (Western blot analysis of LIMP2 in mouse liver tissue lysate with LIMP2 antibody at 1 μg/mL in (A) the absence and (B) presence of blocking peptide.)

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LIMP2, Polyclonal Antibody (Cat# AAA39905)

Full Name

LIMP2 Antibody

Gene Names

SCARB2; AMRF; EPM4; LGP85; CD36L2; HLGP85; LIMP-2; LIMPII; SR-BII

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

LIMP2 Antibody is affinity chromatography purified via peptide column.

Pricing

$545/0.1 mg $1,050/2x0.1 mg $1,530/3x0.1 mg $1,985/4x0.1 mg $2,270/5x0.1 mg

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IF (Immunofluorescence) (Immunofluorescence of NUMB in human lung tissue with NUMB antibody at 20 μg/mL.)

IHC (Immunohiostchemistry) (Immunohistochemistry of NUMB in human lung tissue with NUMB antibody at 5 μg/mL.)

WB (Western Blot) (Western blot analysis of NUMB in mouse lung tissue lysate with NUMB antibody at (A) 0.25 and (B) 0.5 μg/mL.)

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NUMB, Polyclonal Antibody (Cat# AAA39296)

Full Name

NUMB Antibody

Gene Names

NUMB; S171; C14orf41; c14_5527

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

NUMB Antibody is affinity chromatography purified via peptide column.

Pricing

$545/0.1 mg $1,050/2x0.1 mg $1,530/3x0.1 mg $1,985/4x0.1 mg $2,270/5x0.1 mg

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded human stomach using ROCK1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded rat heart using ROCK1 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using ROCK1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

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ROCK1, Polyclonal Antibody (Cat# AAA36763)

Full Name

ROCK1

Gene Names

ROCK1; ROCK-I; P160ROCK

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot

Pricing

$250/0.02 mL $475/0.1 mL $925/2x0.1 mL $1,355/3x0.1 mL $1,755/4x0.1 mL $1,935/5x0.1 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded mouse brain using IL1A antibody at dilution of 1:100 (40x lens).)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human esophagus using IL1A antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using IL1A antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

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IL1A, Polyclonal Antibody (Cat# AAA36900)

Full Name

IL1A Polyclonal Antibody

Gene Names

IL1A; IL1; IL-1A; IL1F1; IL1-ALPHA

Reactivity

Human, Mouse

Applications

Western Blot

Purity

Affinity purification

Pricing

$230/0.02 mL $405/0.1 mL $790/2x0.1 mL $1,145/3x0.1 mL $1,485/4x0.1 mL $1,585/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using WT1 antibody. Blue: DAPI for nuclear staining.)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human liver injury using WT1 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using WT1 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

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WT1, Polyclonal Antibody (Cat# AAA36915)

Full Name

WT1 Polyclonal Antibody

Gene Names

WT1; GUD; AWT1; WAGR; WT33; NPHS4; WIT-2; EWS-WT1

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$230/0.02 mL $405/0.1 mL $790/2x0.1 mL $1,145/3x0.1 mL $1,485/4x0.1 mL $1,585/5x0.1 mL

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 200ug extracts of HepG2 cells using 1ug POLR2D antibody. Western blot was performed from the immunoprecipitate using POLR2D antibody at a dilition of 1:1000.)

IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells using POLR2D antibody.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using POLR2D antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

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POLR2D, Polyclonal Antibody (Cat# AAA36942)

Full Name

POLR2D Polyclonal Antibody

Gene Names

POLR2D; RBP4; RPB16; HSRBP4; HSRPB4

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$230/0.02 mL $405/0.1 mL $790/2x0.1 mL $1,145/3x0.1 mL $1,485/4x0.1 mL $1,585/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human breast cancer using ESR1 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using Estrogen Receptor alpha antibody (AAA36645) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 60s)

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ESR1, Polyclonal Antibody (Cat# AAA36645)

Full Name

ESR1 Polyclonal Antibody

Gene Names

ESR1; ER; ESR; Era; ESRA; ESTRR; NR3A1

Reactivity

Human, Mouse

Applications

Western Blot

Purity

Affinity Purification

Pricing

$230/0.02 mL $405/0.1 mL $790/2x0.1 mL $1,145/3x0.1 mL $1,485/4x0.1 mL $1,585/5x0.1 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded human gastric cancer using PTGIR antibody at dilution of 1:200 (40x lens).)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human stomach using PTGIR antibody at dilution of 1:200 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using PTGIR antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 90s.)

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PTGIR, Polyclonal Antibody (Cat# AAA37294)

Full Name

PTGIR Polyclonal Antibody

Gene Names

PTGIR; IP; PRIPR

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$230/0.02 mL $405/0.1 mL $790/2x0.1 mL $1,145/3x0.1 mL $1,485/4x0.1 mL $1,585/5x0.1 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded rat heart using DBH antibody at dilution of 1:100 (40x lens).)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human stomach using DBH antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of mouse brain, using DBH antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit.Exposure time: 30s.)

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DBH, Polyclonal Antibody (Cat# AAA37184)

Full Name

DBH Polyclonal Antibody

Gene Names

DBH; DBM

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Affinity Purification

Pricing

$230/0.02 mL $405/0.1 mL $790/2x0.1 mL $1,145/3x0.1 mL $1,485/4x0.1 mL $1,585/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence of B9D1 in 293 cells with B9D1 antibody at 20 μg/mL.)

ICC (Immunocytochemistry) (Immunocytochemistry of B9D1 in 293 cells with B9D1 antibody at 5 μg/mL.)

WB (Western Blot) (Western blot analysis of B9D1 in 293 cell lysate with B9D1 antibody at 1 μg/mL in (A) the absence and (B) the presence of blocking peptide.)

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B9D1, Polyclonal Antibody (Cat# AAA40244)

Full Name

B9D1 Antibody

Gene Names

B9D1; B9; MKS9; EPPB9; MKSR1

Reactivity

Human

Applications

Immunofluorescence, Immunocytochemistry, Western Blot

Purity

B9D1 Antibody is affinity chromatography purified via peptide column.

Pricing

$545/0.1 mg $1,050/2x0.1 mg $1,530/3x0.1 mg $1,985/4x0.1 mg $2,270/5x0.1 mg

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded mouse brain using CRYM antibody at dilution of 1:100 (40x lens).)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded rat brain using CRYM antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using CRYM antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

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CRYM, Polyclonal Antibody (Cat# AAA37466)

Full Name

CRYM Polyclonal Antibody

Gene Names

CRYM; THBP; DFNA40

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$230/0.02 mL $405/0.1 mL $790/2x0.1 mL $1,145/3x0.1 mL $1,485/4x0.1 mL $1,585/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using PSMC4 antibody. Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using PSMC4 antibody at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit.Exposure time: 90s.)

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PSMC4, Polyclonal Antibody (Cat# AAA37545)

Full Name

PSMC4 Polyclonal Antibody

Gene Names

PSMC4; S6; RPT3; TBP7; TBP-7; MIP224

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Affinity Purification

Pricing

$230/0.02 mL $405/0.1 mL $790/2x0.1 mL $1,145/3x0.1 mL $1,485/4x0.1 mL $1,585/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescent analysis of MMP3 staining in A431 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of MMP3 staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of MMP3 expression in HeLa (A), A431 (B), mouse heart (C), PC12 (D) whole cell lysates.)

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MMP3, Polyclonal Antibody (Cat# AAA104576)

Full Name

Anti-MMP3 Antibody

Gene Names

MMP3; SL-1; STMY; STR1; CHDS6; MMP-3; STMY1

Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$230/0.03 mL $250/0.05 mL $320/0.1 mL $410/0.2 mL $800/2x0.2 mL $1,160/3x0.2 mL $1,505/4x0.2 mL $1,630/5x0.2 mL

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ICC (Immunocytochemistry) (Anti- PARK7 Picoband antibody, AAA46579,ICCICC: A549 Cell)

IHC (Immunohistochemistry) (Anti- PARK7 Picoband antibody, AAA46579, IHC(P)IHC(P): Human Pancreatic Cancer Tissue)

IHC (Immunohistochemisry) (Anti- PARK7 Picoband antibody, AAA46579, IHC(P)IHC(P): Rat Pancreas Tissue)

IHC (Immunohiostchemistry) (Anti- PARK7 Picoband antibody, AAA46579, IHC(P)IHC(P): Mouse Pancreas Tissue)

WB (Western Blot) (Anti- PARK7 Picoband antibody, AAA46579, Western blottingAll lanes: Anti PARK7 (AAA46579) at 0.5ug/mlLane 1: PANC Whole Cell Lysate at 40ugLane 2: U20S Whole Cell Lysate at 40ugLane 3: SMMC Whole Cell Lysate at 40ugLane 4: HELA Whole Cell Lysate at 40ugPredicted bind size: 20KDObserved bind size: 20KD)

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PARK7/DJ1, Polyclonal Antibody (Cat# AAA46579)

Full Name

Anti-PARK7/DJ1 Antibody

Gene Names

PARK7; DJ1; DJ-1; HEL-S-67p

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Immunogen Affinity Purified

Pricing

$215/0.01 mg $485/0.1 mg (Carrier Free) $485/0.1 mg $705/0.1 mg (Biotin) $705/0.1 mg (HRP) $705/0.1 mg (FITC) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Cy3) $705/0.1 mg (Dylight488) $810/0.1 mg (PE) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $2,005/5x0.1 mg

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IHC (Immunohistochemistry) (Anti- HSD11B2 antibody, IHC(P)IHC(P): Human Placenta Tissue)

IHC (Immunohistochemisry) (Anti- HSD11B2 antibody, IHC(P)IHC(P): Rat Pancreas Tissue)

IHC (Immunohiostchemistry) (Anti- HSD11B2 antibody, IHC(P)IHC(P): Mouse Pancreas Tissue)

WB (Western Blot) (Anti- HSD11B2 antibody, Western blotting)

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HSD11B2, Polyclonal Antibody (Cat# AAA46463)

Full Name

Anti-HSD11B2 Antibody

Gene Names

HSD11B2; AME; AME1; HSD2; HSD11K; SDR9C3

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Immunogen Affinity Purified

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

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IHC (Immunohistochemisry) (Anti-ADAM19 antibody, AAA45408, IHC(P)IHC(P): Human Lung Cancer Tissue)

IHC (Immunohiostchemistry) (Anti-ADAM19 antibody, AAA45408, IHC(P)IHC(P): Rat Spleen Tissue)

WB (Western Blot) (Anti-ADAM19 antibody, AAA45408, Western blottingLane 1: Rat Spleen Tissue LysateLane 2: Rat Intestine Tissue LysateLane 3: Rat Brain Tissue LysateLane 4: HELA Cell Lysate)

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ADAM19, Polyclonal Antibody (Cat# AAA45408)

Full Name

Anti-ADAM19 antibody

Gene Names

ADAM19; MLTNB; FKSG34; MADDAM

Reactivity

Human

Applications

Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

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IHC (Immunohistochemistry) (Anti-B MyB antibody, AAA45422, IHC(P)IHC(P): Human Tonsil Tissue)

ICC (Immunocytochemistry) (Anti-B MyB antibody, AAA45422, ICCICC: A549 Cell)

WB (Western Blot) (Anti-B MyB antibody, AAA45422, Western blottingLane 1: Rat Spleen Tissue LysateLane 2: Rat Thymus Tissue LysateLane 3: Rat Brain Tissue LysateLane 4: HELA Cell LysateLane 5: COLO320 Cell LysateLane 6: MCF-7 Cell Lysate)

IHC (Immunohiostchemistry) (Anti-B MyB antibody, AAA45422, IHC(P)IHC(P): Rat Brain Tissue)

WB (Western Blot) (Anti-B MyB antibody, AAA45422, Western blottingRecombinant Protein Detection Source: E.coli derived -recombinant Human MYBL2, 46.8KD(162aa tag+ M1-L245)Lane 1: Recombinant Human MYBL2 Protein 10ngLane 2: Recombinant Human MYBL2 Protein 5ngLane 3: Recombinant Human MYBL2 Protein 2.5ng)

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B MyB, Polyclonal Antibody (Cat# AAA45422)

Full Name

Anti-B MyB antibody

Gene Names

MYBL2; BMYB; B-MYB

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg (Carrier Free) $485/0.1 mg $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (Dylight594) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (PE) $810/0.1 mg (iFluor647) $2,005/5x0.1 mg

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ICC (Immunocytochemistry) (Anti- Peroxiredoxin 5 Picoband antibody, AAA46292, ICCICC: SMMC Cell)

IHC (Immunohistochemistry) (Anti- Peroxiredoxin 5 Picoband antibody, AAA46292, IHC(P)IHC(P): Human Lung Cancer Tissue)

IHC (Immunohistochemisry) (Anti- Peroxiredoxin 5 Picoband antibody, AAA46292, IHC(P)IHC(P): Rat Intestine Tissue)

IHC (Immunohiostchemistry) (Anti- Peroxiredoxin 5 Picoband antibody, AAA46292, IHC(P)IHC(P): Mouse Intestine Tissue)

WB (Western Blot) (Anti- Peroxiredoxin 5 Picoband antibody, AAA46292, Western blottingAll lanes: Anti Peroxiredoxin 5 (AAA46292) at 0.5ug/mlLane 1: A549 Whole Cell Lysate at 40ugLane 2: Rat Brain Tissue Lysate at 50ugLane 3: Mouse Brain Tissue Lysate at 50ugPredicted bind size: 22KDObserved bind size: 22KD)

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Peroxiredoxin 5, Polyclonal Antibody (Cat# AAA46292)

Full Name

Anti-Peroxiredoxin 5 Antibody

Gene Names

PRDX5; PLP; ACR1; B166; PRXV; PMP20; PRDX6; prx-V; SBBI10; AOEB166; HEL-S-55

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Immunogen Affinity Purified

Pricing

$215/0.01 mg $485/0.1 mg (Carrier Free) $485/0.1 mg $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (Dylight594) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (PE) $810/0.1 mg (iFluor647) $2,005/5x0.1 mg

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WB (Western Blot) (Western blot analysis of A:Non-transfected lysate; B:HLA-DMB transfected lysate using a 12 % SDS PAGE gel and HLA DMB antibody at a dilution of 1:500)

IHC (Immunohistochemistry) (Immunohistochemical staining of paraffin-embedded Colon ca using HLA DMB antibody at a dilution of 1:250)

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HLA DMB, Polyclonal Antibody (Cat# AAA107615)

Full Name

HLA DMB antibody

Applications

Western Blot, Immunohistochemistry

Purity

HLA DMB antibody was purified by antigen-affinity chromatography

Pricing

$1,375/0.1 mL $2,675/2x0.1 mL $3,905/3x0.1 mL $5,065/4x0.1 mL $5,950/5x0.1 mL

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FCM/FACS (Flow Cytometry) (MASTL Antibody flow cytometric analysis of HepG2 cells (bottom histogram) compared to a negative control cell (top histogram).FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.)

IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human hepatocarcinoma reacted with MASTL Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.)

WB (Western Blot) (Western blot analysis of MASTL Antibody in HepG2, MDA-MB231, 293, Hela cell line lysates (35ug/lane). MASTL (arrow) was detected using the purified Pab.)

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MASTL, Polyclonal Antibody (Cat# AAA284981)

Full Name

MASTL Antibody

Gene Names

MASTL; GW; GWL; THC2; hGWL; MAST-L; GREATWALL

Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.