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SDS-PAGE (K562 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CD43(ABT-CD43) antibody. The HRP-conjugated Goat anti-M)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-GBA antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-GBA antibody. Counter stained with hematoxylin.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-GBA antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-GBA antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-GBA antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GBA antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-GBA antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of GBA on diffewrent cells lysates using anti-GBA antibody at 1/1, 000 dilution. Positive control: Lane 1: HepG2 Lane 2: 293T)
WB (Western Blot) (Western blot analysis of HNF4A over-expressed 293 cell line, cotransfected with HNF4A Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with HNF4A monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged HNF4A is ~1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HNF4A on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of HNF4A expression in transfected 293T cell line by HNF4A monoclonal antibody. Lane 1: HNF4A transfected lysate (51.6kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (HNF4A monoclonal antibody Western Blot analysis of HNF4A expression in Jurkat.)
WB (Western Blot) (HNF4A monoclonal antibody Western Blot analysis of HNF4A expression in HepG2)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat liver tissue using Prohibitin Rabbit mAb (AAA28503) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using Prohibitin Rabbit mAb (AAA28503) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Prohibitin Rabbit mAb (AAA28503) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using Prohibitin Rabbit mAb (AAA28503) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human liver tissue using Prohibitin Rabbit mAb (AAA28503) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Prohibitin Rabbit mAb (AAA28503) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
WB (Western Blot) (Western blot analysis of various lysates using Prohibitin Rabbit mAb (AAA28503) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 5s.)
ICC (Immunocytochemistry) (ICC staining ICAM1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining ICAM1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ICAM1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-ICAM1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-ICAM1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-ICAM1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of ICAM1 on different lysates using anti-ICAM1 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: HUVEC Lane 3: Raji)
FCM (Flow Cytometry) (Flow cytometric analysis of MCF-7 cells with SCF antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining SCF in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining SCF in PANC-1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining SCF in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-SCF antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SCF antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of SCF on Raji cells lysates using anti-SCF antibody at 1/1, 000 dilution.)
IHC (Immunohistochemistry) (Immunohistochemistry (FFPE) analysis of human tonsil stained with C2399-07G2, biotinylated anti-mouse IgG and Streptavidin Alkaline Phosphatase (red). Nuclei were counterstained with hematoxylin.)
IHC (Immunohistochemistry) (immunohistochemical analysis of an acetone fixed, frozen human colon section using C2399-07G2.)
CD45 (CD45 Antigen, B220, GP180, Leukocyte Common Antigen, LCA, L-CA, LY5, LY-5, Protein Tyrosine Phosphatase Receptor Type C, Protein Tyrosine Phosphatase Receptor Type C Polypeptide, PTPRC, T200, T200 Glycoprotein) (FITC)
FCM (Flow Cytometry) (Figure-6: Epitope binding study by flow cytometric analysis. MCF-7 cells expressing HER2 antigen were treated with Herceptin and Samceptin (2 ug/10^6 Cells). Surface staining was done using FITC conjugated antibodies.)
FCM (Flow Cytometry) (Figure-5: Epitope binding study by flow cytometric analysis. BT-474 cells expressing HER2 antigen were treated with Herceptin and Samceptin (2 ug/10^6 Cells). Surface staining was done using FITC conjugated antibodies.)
SDS-PAGE (Figure-4: Reducing SDS-PGE of Samceptin (Lane-1) in comparison with Herceptin (Lane-2). Lanes were overloaded to show the presence of any other protein bands than Samceptin. Both heavy and light chains are well separated.)
Activity (Figure-3: SEC-HPLC analysis of Herceptin and Samceptin. Based on this analysis, Samceptin is highly pure and purity estimated to be >98%)
Activity (Figure-2: Antiproliferative activity of Samceptin was assayed in comparison with Herceptin using ADCC Reporter Bioassay Kit from Promega in MCF-7 cells. Result indicated that Samceptin potency is at par with Herceptin.)
Activity (Figure-1: Antiproliferative activity of Samceptin was assayed in comparison with Herceptin using ADCC Reporter Bioassay Kit in SK-BR-3 cells. Result indicated that Samceptin potency is at par with Herceptin.)
FCM (Flow Cytometry) (Flow cytometric analysis of 293T cells with XBP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining XBP1 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining XBP1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining XBP1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-XBP1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-XBP1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of XBP1 on different cell lysates using anti-XBP1 antibody at 1/1, 000 dilution. Positive control: Lane 1:HepG2 Lane 2: 293T)
FCM (Flow Cytometry) (Flow cytometric analysis of SH-SY-5Y cells with PDK2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-PDK2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human fetal skeletal muscle tissue using anti-PDK2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PDK2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-PDK2 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of PDK2 on rat heart tissue lysate using anti-PDK2 antibody at 1/500 dilution.)
FCM (Flow Cytometry) (Flow cytometric analysis of SH-SY5Y cells with SATB2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
ICC (Immunocytochemistry) (ICC staining SATB2 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining SATB2 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining SATB2 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-SATB2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-SATB2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat large intestine tissue using anti-SATB2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-SATB2 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of SATB2 on THP-1 cell using anti-SATB2 antibody at 1/500 dilution.)
Application Data (Detection limit for recombinant GST tagged PGR is ~0.3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PGR on MCF-7 cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody, 131852, to PGR on formalin-fixed paraffin-embedded human breast cancer. [antibody concentration 3ug/ml])
WB (Western Blot) (PGR monoclonal antibody, 131852, Western Blot analysis of PGR expression in MCF-7.)
WB (Western Blot) (PGR monoclonal antibody, 131852, Western Blot analysis of PGR expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen, 131852 (37.84kD))
WB (Western Blot) (Western blot analysis of MUTYH over-expressed 293 cell line, cotransfected with MUTYH Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MUTYH monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged MUTYH is ~1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of MUTYH transfected lysate using anti-MUTYH monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with MUTYH rabbit polyclonal antibody.)
WB (Western Blot) (Western Blot analysis of MUTYH expression in transfected 293T cell line by MUTYH monoclonal antibody. Lane 1: MUTYH transfected lysate (59.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (MUTYH monoclonal antibody Western Blot analysis of MUTYH expression in HeLa NE.)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
FCM (Flow Cytometry) (Flow cytometric analysis of SH-SY-5Y cells with MYLK antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining MYLK in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining MYLK in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining MYLK in L6 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining MYLK in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse smooth muscle tissue using anti-MYLK antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-MYLK antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat smooth muscle tissue using anti-MYLK antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of MYLK on human lung lysates using anti-MYLK antibody at 1/5, 000 dilution.)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with PKC beta 2 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
ICC (Immunocytochemistry) (ICC staining PKC beta 2 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-PKC beta 2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-PKC beta 2 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PKC beta 2 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of PKC beta 2 on Raji cell lysates using anti-PKC beta 2 antibody at 1/1, 000 dilution.)
FCM (Flow Cytometry) (Flow cytometric analysis of A549 cells with alpha Actinin 4 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
ICC (Immunocytochemistry) (ICC staining alpha Actinin 4 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining alpha Actinin 4 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining alpha Actinin 4 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-alpha Actinin 4 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-alpha Actinin 4 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of alpha Actinin 4 on different lysates using anti-alpha Actinin 4 antibody at 1/500 dilution. Positive control: Lane 1: Hela Lane 2: PC-12 Lane 3: NIH-3T3 Lane 4: Rat liver tissue Lane 5: A431 Lane 6: HepG2)
FCM (Flow Cytometry) (Flow cytometric analysis of NIH/3T3 cells with eIF4EBP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
ICC (Immunocytochemistry) (ICC staining eIF4EBP1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining eIF4EBP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-eIF4EBP1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-eIF4EBP1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of eIF4EBP1 on different lysates using anti-eIF4EBP1 antibody at 1/1, 000 dilution. Positive control: Lane 1: K562 Lane 2: HepG2 Lane 3: 293)
WB (Western Blot) (RAD18 monoclonal antibody, Western Blot analysis of RAD18 expression in Hela NE.)
WB (Western Blot) (Western blot analysis of RAD18 over-expressed 293 cell line, cotransfected with RAD18 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with RAD18 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged RAD18 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RAD18 on HeLa cell. [antibody concentration 25ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RAD18 on formalin-fixed paraffin-embedded human testis. [antibody concentration 1.5ug/ml].)
WB (Western Blot) (Western Blot analysis of RAD18 expression in transfected 293T cell line by RAD18 monoclonal antibody. Lane 1: RAD18 transfected lysate (56.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (37kD).)
IHC (Immunohistochemistry) (Immunochemical staining MSH6 in Ferret spleen with rabbit monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistchemistry) (Immunochemical staining MSH6 in rat kidney with rabbit monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry) (Immunochemical staining MSH6 in rat spleen with rabbit monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry) (Immunochemical staining MSH6 in mouse kidney with rabbit monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistchemistry) (Immunochemical staining MSH6 in mouse spleen with rabbit monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry) (Immunochemical staining MSH6 in cynomolgus kidney with rabbit monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)
IHC (Immunohistochemistry) (Immunochemical staining MSH6 in cynomolgus spleen with rabbit monoclonal antibody at 1:200 dilution, formalin-fixed paraffin embedded sections.)
IF (Immunofluorescence) (Immunofluorescence staining of MSH6 in HeLa cells. Cells were fixed with 4% PFA, permeabilzed with 0.1% Triton X-100 in PBS,blocked with 10% serum, and incubated with rabbit anti-human MSH6 monoclonal antibody (dilution ratio 1:60) at 4 degree C overnight. Then cells were stained with the Alexa Fluor488-conjugated Goat Anti-rabbit IgG secondary antibody (green) and counterstained with DAPI (blue).Positive staining was localized to Nucleus.)
IHC (Immunohistochemistry) (Immunochemical staining MSH6 in human colon carcinoma with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).)
IHC (Immunohistochemistry) (Immunochemical staining MSH6 in human rectal cancer with rabbit monoclonal antibody (1:200, formalin-fixed paraffin embedded sections).)
WB (Western Blot) (ACTB monoclonal antibody (M03), clone 1E7. Western Blot analysis of ACTB expression in Raw 264.7.)
WB (Western Blot) (ACTB monoclonal antibody (M03), clone 1E7. Western Blot analysis of ACTB expression in PC-12.)
WB (Western Blot) (ACTB monoclonal antibody (M03), clone 1E7. Western Blot analysis of ACTB expression in NIH/3T3.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ACTB on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ACTB on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml])
WB (Western Blot) (ACTB monoclonal antibody (M03), clone 1E7. Western Blot analysis of ACTB expression in Jurkat (Cat # L017V1).)
ICC (Immunocytochemistry) (ICC staining GST3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining GST3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining GST3 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse fallopian tube tissue using anti-GST3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-GST3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-GST3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-GST3 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of GST3 on mouse liver tissue (1) and A549 cell (2) lysate using anti-GST3 antibody at 1/500 dilution.)
FCM (Flow Cytometry) (Flow cytometric analysis of PC-3M cells with hUPF1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining hUPF1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining hUPF1 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining hUPF1 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti- hUPF1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti- hUPF1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue using anti-hUPF1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon tissue using anti- hUPF1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of hUPF1 on PC-3M cell lysate using anti-hUPF1 antibody at 1/1, 000 dilution.)
ICC (Immunocytochemistry) (ICC staining Actin in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Actin in AGS cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Actin in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse smooth muscle tissue using anti-Actin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse cardiac muscle tissue using anti-Actin antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Actin on different lysates using anti-Actin antibody at 1/1, 000 dilution. Positive control: Lane 1:Hybrid fish (crucian-carp) brain tissue Lane 2:Hybrid fish (crucian-carp) kidney tissue )
WB (Western Blot) (Western blot analysis of Actin on different lysates using anti-Actin antibody at 1/1, 000 dilution. Positive control: Lane 1: zebrafish Lane 2: Hela Lane 3: A431 Lane 4: Jurkat)
IF (Immunofluorescence) (Immunofluorescence analysis of Mouse-colon tissue. 1.CD4 Monoclonal Antibody(11A1)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1.CD4 Monoclonal Antibody(11A1) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue. 1.CD4 Monoclonal Antibody(11A1) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human-stomach tissue. 1.CD4 Monoclonal Antibody(11A1) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human pancreas. 1. Antibody was diluted at 1:200(4 degree overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human pancreas. 1. Antibody was diluted at 1:200(4 degree overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Amygdala. 1. Antibody was diluted at 1:400(4 degree overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)
FCM (Flow Cytometry) (Flow cytometric analysis of Jurkat cells with USP14 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining USP14 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining USP14 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining USP14 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded rat epididymis tissue using anti-USP14 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-USP14 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-USP14 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-USP14 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of USP14 on Zebrafish tissue lysates using anti-USP14 antibody at 1/200 dilution.)
WB (Western Blot) (Western blot analysis of USP14 on K562 (1) and Hela (2) cell lysate using anti-USP14 antibody at 1/1, 000 dilution.)
FCM (Flow Cytometry) (Overlay Peak curve showing 293F cells transfected with GST stained with AAA28065 (red line) at 1:189. The cells were fixed in 4% formaldehyde and permeated by 0.2% TritonX-100. Then 10% normal goat serum was Incubated to block non-specific protein-protein interactions followed by the antibody (1?g/1*106cells) for 1 h at 4 degree C. The secondary antibody used was FITC-conjugated Goat Anti-Mouse IgG(H+L) at 1/100 dilution for 30min at 4 degree C. Isotype control antibody (green line) was mouse IgG2b (1?g/1*106cells) used under the same conditions. Acquisition of >10,000 events was performed.)
IP (Immunoprecipitation) (Immunoprecipitating GST in transfected-293F whole cell lysate Lane 1: Mouse control IgG instead of AAA28065 in 293F cell lysate Transfected with GST Lane 2: AAA28065 (5?g) + Transfected-293F whole cell lysate (500?g) Lane 3: Transfected-293F whole cell lysate (10?g) For western blotting, the blot was detected with AAA28065 at 1:2000, and a HRP-conjugated Protein G antibody was used as the secondary antibody at 1:50000)
IF (Immunofluorescence) (Immunofluorescence staining of 293F transfected cells with AAA28065 at 1:94.5, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).)
WB (Western Blot) (Western BlotPositive WB detected in: 25ng recombinant protein with GST tag from E.coliGST antibody at 1:10000, 1:20000, 1:40000, 1:80000, 1:160000, 1:320000, 1:640000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 26 KDaObserved band size: 26 KDaExposure time: 5min)
WB (Western Blot) (Western BlotPositive WB detected in: Recombinant protein with GST tag from E.coli at 50ng, 25ng, 12.5ng, 6.25ng, 3.125ng, 1.5625ngAll lanes: GST antibody at 1:10000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 26 KDaObserved band size: 26 KDaExposure time: 5min)
WB (Western Blot) (Western BlotPositive WB detected in: 50ng Recombinant protein with GST tag from E.coliAll lanes: GST antibody at 1:2000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 26, 102.9, 75.6 KDaObserved band size: 26, 103, 76 KDaExposure time: 5min)
WB (Western Blot) (Western BlotPositive WB detected in: 25ng Recombinant protein with GST tag from E.coliAll lanes: GST antibody at 1:10000SecondaryGoat polyclonal to mouse IgG at 1/50000 dilutionPredicted band size: 32.6, 30.1, 29.5, 37.4, 30.4, 60.5, 38.1, 61.6 KDaObserved band size: 31, 30, 31, 37, 30, 60, 37, 61 KDaExposure time: 5min)
ICC (Immunocytochemistry) (ICC staining Prohibitin in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Prohibitin in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Prohibitin antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Prohibitin on hybrid fish (crucian-carp) liver tissue lysate using anti-Prohibitin antibody at 1/500 dilution.)
WB (Western Blot) (Western blot analysis of Prohibitin on different lysates using anti-Prohibitin antibody at 1/1, 000 dilution. Positive control: Lane 1: 293 Lane 2: Jurkat Lane 3: Mouse kidney Lane 4: HepG2)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Smad5 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining Smad5 in PC-3M cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Smad5 in A549 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Smad5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Smad5 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Smad5 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Smad5 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Smad5 on PC-3M cell lysates using anti-Smad5 antibody at 1/1, 000 dilution.)
Application Data (Detection limit for recombinant GST tagged HMGB2 is approximately 0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HMGB2 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HMGB2 on HeLa cell. [antibody concentration 10 ug/ml])
WB (Western Blot) (HMGB2 monoclonal antibody (M04), clone 3D2 Western Blot analysis of HMGB2 expression in Hela S3 NE (Cat # L013V3).)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HMGB2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to HMGB2 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])
CD28 (CD28 Antigen, CD28 Molecule, MGC138290, T cell Antigen CD28, T cell Specific Surface Glycoprotein, T cell Specific Surface Glycoprotein CD28, Tp44) (PE)
WB (Western Blot) (Detection of human and mouse beta-Catenin by western blot. Samples: Whole cell lysate (50 ug) from Hep-G2, LNCaP, K-562, GaMG, Jurkat, A-549, MCF-7, HeLa, TCMK-1, and NIH 3T3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-beta-Catenin recombinant monoclonal antibody (AAA23827 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds.)
IP (Immunoprecipitation) (Detection of human beta Catenin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Hep-G2 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-beta Catenin recombinant monoclonal antibody (AAA23827 lot 1) used for IP at 20 ul per reaction. beta Catenin was also immunoprecipitated by rabbit anti-beta Catenin antibodies . For blotting immunoprecipitated beta Catenin, AAA23827 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IP (Immunoprecipitation) (Detection of human beta Catenin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-beta Catenin recombinant monoclonal antibody (AAA23827 lot 1) used for IP at 20 ul per reaction. beta Catenin was also immunoprecipitated by rabbit anti-beta Catenin antibodies . For blotting immunoprecipitated beta Catenin, AAA23827 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)
IHC (Immunohistchemistry) (Detection of human beta Catenin in FFPE prostate carcinoma by immunohistochemistry-IF. Antibody: Rabbit anti-beta Catenin recombinant monoclonal antibody (AAA23827 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI.)
IHC (Immunohistochemistry) (Detection of mouse beta Catenin in FFPE plasmacytoma by IHC. Antibody: Rabbit anti-beta Catenin recombinant monoclonal antibody (AAA23827 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
IHC (Immunohistochemistry) (Detection of human beta Catenin in FFPE colon carcinoma by IHC. Antibody: Rabbit anti-beta Catenin recombinant monoclonal antibody (AAA23827 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
ICC (Immunocytochemistry) (Detection of human beta Catenin in FFPE OVCAR-3 cells by ICC. Antibody: Rabbit anti-beta Catenin recombinant monoclonal antibody (AAA23827 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)
FCM (Flow Cytometry) (Detection of human beta Catenin (shaded) in FaDu cells by flow cytometry. Antibody: Rabbit anti-beta Catenin recombinant monoclonal antibody (AAA23827) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry) (Detection of mouse beta Catenin (shaded) in FaDu cells by flow cytometry. Antibody: Rabbit anti-beta Catenin recombinant monoclonal antibody (AAA23827) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Transferrin Receptor antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-Transferrin Receptor antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Transferrin Receptor antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Transferrin Receptor antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Transferrin Receptor antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Transferrin Receptor on mouse placenta lysates using anti-Transferrin Receptor antibody at 1/1, 000 dilution.)
Application Data (Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 followed by horseradish peroxidase Goat anti Mouse IgG2a antibody as a detection reagent. Medium power)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD3:RPE)
Application Data (Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 followed by horseradish peroxidase Goat anti Mouse IgG2a antibody as a detection reagent. Low power)
Application Data (ELISA analysis of human CD3 expression using Rat anti Human CD3ε, clone CD3-12 as a capture reagent and biotinylated Mouse anti Human CD3, clone UCHT1 as a detection reagent with recombinant human CD3 as antigen to produce the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . A serum (green) sample is included undiluted)
Application Data (Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 followed by horseradish peroxidase Goat anti Mouse IgG2a antibody as a detection reagent. High power)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD3:Alexa Fluor 405)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD3)
Application Data (Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 followed by horseradish peroxidase Goat anti Mouse IgG2a antibody as a detection reagent. High power)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD3: RPE- Alexa Fluor 750)
Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD3: FITC)
Application Data (Analysis of Protein Array containing more than 19, 000 full-length human proteins using CD10 Mouse Monoclonal Antibody (MME/1870)Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)
SDS-PAGE (SDS -PAGE Analysis Purified CD10 Mouse Monoclonal Antibody (MME/1870).Confirmation of Purity and Integrity)
WB (Western Blot) (Western blot analysis of kidney tissue lysate using CD10 Mouse Monoclonal Antibody (MME/1870).)
IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded Human Renal Cell Carcinoma stained with CD10 Mouse Monoclonal Antibody (MME/1870).)
IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded Human Colon stained with CD10 Mouse Monoclonal Antibody (MME/1870).)
IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded Human Prostate stained with CD10 Mouse Monoclonal Antibody (MME/1870).)
IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded Human Kidney stained with CD10 Mouse Monoclonal Antibody (MME/1870).)
WB (Western Blot) (Western blot analysis of NMI over-expressed 293 cell line, cotransfected with NMI Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with NMI monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged NMI is ~0.03ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NMI on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of NMI expression in transfected 293T cell line by NMI monoclonal antibody. Lane 1: NMI transfected lysate (35.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (NMI monoclonal antibody Western Blot analysis of NMI expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TIMP2 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TIMP2 on HeLa cell. [antibody concentration 10 ug/ml])
Application Data (Detection limit for recombinant GST tagged TIMP2 is approximately 0.1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TIMP2 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TIMP2 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml])
WB (Western Blot) (TIMP2 monoclonal antibody (M03), clone 1C3 Western Blot analysis of TIMP2 expression in HeLa (Cat # L013V1).)
IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to FOXA2 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 0.5 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FOXA2 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 0.5 ug/ml])
Application Data (Detection limit for recombinant GST tagged FOXA2 is approximately 0.3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA2 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA2 on HeLa cell. [antibody concentration 10 ug/ml])
WB (Western Blot) (FOXA2 monoclonal antibody (M12), clone 6C12 Western Blot analysis of FOXA2 expression in K-562.)
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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