Monoclonal Antibodies

Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.

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Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD13: Alexa Fluor 647)

Application Data (Immunofluorescence staining of human tonsil cryosection with Mouse anti Human CD13 antibody, clone WM15 (red in A) and Mouse anti CD21 antibody, clone LB21 (green in B). The merged image is in C with nuclei counterstained blue using DAPI. High power.)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD13: APC)

Application Data (Immunofluorescence staining of human tonsil cryosection with Mouse anti Human CD13 antibody, clone WM15 (red in A) and Mouse anti CD21 antibody, clone LB21 (green in B). The merged image is in C with nuclei counterstained blue using DAPI. Medium power.)

Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD13)

Application Data (Immunofluorescence staining of human tonsil cryosection with Mouse anti Human CD13 antibody, clone WM15 (red in A) and Mouse anti CD21 antibody, clone LB21 (green in B). The merged image is in C with nuclei counterstained blue using DAPI. Low power.)

Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD13 antibody, clone WM15 followed by Histar Detection System. Medium power)

Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD13:RPE)

Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD13:FITC)

Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD13 antibody, clone WM15 followed by Histar Detection System. Medium power)

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CD13, Monoclonal Antibody (Cat# AAA12026)

Full Name

MOUSE ANTI HUMAN CD13:RPE

Gene Names

ANPEP; APN; CD13; LAP1; P150; PEPN; GP150

Applications

Flow Cytometry

Pricing

$625/100 Tests $1,190/2x100 Tests $1,720/3x100 Tests $2,225/4x100 Tests $2,490/5x100 Tests

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IHC (Immunohistochemistry) (CD44 antibody immunohistochemistry analysis in formalin fixed and paraffin embedded human esophagus carcinoma followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of the CD44 antibody for immunohistochemistry. Clinical relevance has not been evaluated.)

IHC (Immunohistchemistry) (AAA14166 staining CD44 in human skin tissue sections by Immunohistochemistry (IHC-P- paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37u00b0C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (AAA14166 staining CD44 in human lung adenocarcinoma tissue sections by Immunohistochemistry (IHC-P- paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37u00b0C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (AAA14166 staining CD44 in human skin tissue sections by Immunohistochemistry (IHC-P- paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody (1/25) for 1 hours at 37u00b0C. A undiluted biotinylated goat polyvalent antibody was used as the secondary antibody.)

Application Data (AAA14166 staining CD44 in human lung adenocarcinoma tissue sections by Immunohistochemistry (IHC-P- paraformaldehyde-fixed, paraffin-embedded sections). Tissue was fixed with formaldehyde and blocked with 3% BSA for 0. 5 hour at room temperature; antige)

Application Data (CD44 antibody confocal immunofluorescent analysis with hela cell. 0.01 mg/ml primary antibody was followed by PE-conjugated goat anti-mouse lgG (whole molecule). PE emits red fluorescence. DAPI was used to stain the cell nuclear (blue).)

WB (Western Blot) (Western blot detection of CD44 in A549 and Hela cells using CD44 Mouse mAb(dilution 1:1000).Predicted band size:81kDa.Observed band size:81kDa.)

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CD44, Monoclonal Antibody (Cat# AAA14166)

Full Name

Anti-CD44 Mouse mAb

Gene Names

CD44; IN; LHR; MC56; MDU2; MDU3; MIC4; Pgp1; CDW44; CSPG8; HCELL; HUTCH-I; ECMR-III

Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Immunofluorescence, Flow Cytometry

Purity

Purified through a protein G column, eluted with high and low pH buffers and neutralized immediately, followed by dialysis against PBS.

Pricing

$565/0.1 mL $1,070/2x0.1 mL $1,545/3x0.1 mL $1,900/5x0.1 mL $1,995/4x0.1 mL

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Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b)

Application Data (Staining of mouse peritoneal macrophages cells with Rat anti Mouse CD11b:Alexa Fluor 488)

Application Data (Staining of total mouse peritoneal exudate cells demonstrating labelling of macrophages with Rat anti Mouse CD11b:RPE)

Application Data (Published customer image: Immunocytochemical characterisation of cultures of mouse microglia. Cultures of microglia were immunostained with anti-CD11b (a) and anti-GFAP (c). Cultures of astrocytes were immunostained with anti-CD11b (c) and anti-GFAP (d). All cells were also counterstained with DAPI (blue) to identify the cells' nuclei.From: Ferger et al. Journal of Neuroinflammation 2010 7:45)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b:RPE)

Application Data (Staining of mouse peritoneal macrophages cells with Rat anti Mouse CD11b:FITC)

Application Data (Immunoperoxidase staining of mouse lymph node cryosection using Rat anti Mouse CD11b antibody followed by horseradish peroxidase conjugated Goat anti Rat IgG . High power)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b: Alexa Fluor 647)

Application Data (Immunoperoxidase staining of mouse lymph node cryosection using Rat anti Mouse CD11b antibody followed by horseradish peroxidase conjugated Goat anti Rat IgG . Medium power)

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CD11b, Monoclonal Antibody (Cat# AAA12219)

Full Name

RAT ANTI MOUSE CD11b:Low Endotoxin

Gene Names

Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik

Applications

Immunohistochemistry, Flow Cytometry, Functional Assay, Immunofluorescence, Immunoprecipitation, Immunohistochemistry

Pricing

$915/0.5 mg $1,755/2x0.5 mg $2,550/3x0.5 mg $3,300/4x0.5 mg $3,790/5x0.5 mg

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WB (Western Blot) (Detection of human CD206/Mannose Receptor by western blot. Samples: Whole cell lysate (50 ug) from HeLa, HEK293T, MUTZ-3, Hep-G2, and MCF-7 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD206/Mannose Receptor recombinant monoclonal antibody (AAA23836 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 1 second. Lower Panel: Rabbit anti-COPB2 antibody .)

IP (Immunoprecipitation) (Detection of human CD206/Mannose Receptor by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MUTZ-3 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD206/Mannose Receptor recombinant monoclonal antibody (AAA23836 lot 1) used for IP at 6 ul per reaction. CD206/Mannose Receptor was also immunoprecipitated by rabbit anti-CD206/Mannose Receptor antibody 1507A-3G8. For blotting immunoprecipitated CD206/Mannose Receptor, AAA23836 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)

IHC (Immunohistochemistry) (Detection of human CD206/Mannose Receptor in FFPE ovarian carcinoma by IHC. Antibody: Rabbit anti-CD206/Mannose Receptor recombinant monoclonal antibody (AAA23836 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

IHC (Immunohistochemistry) (Detection of human CD206/Mannose Receptor in FFPE non-Hodgkin's lymphoma by IHC. Antibody: Rabbit anti-CD206/Mannose Receptor recombinant monoclonal antibody (AAA23836 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

IHC (Immunohistochemistry) (Detection of human CD206/Mannose Receptor in FFPE lung carcinoma pleural effusion by IHC. Antibody: Rabbit anti-CD206/Mannose Receptor recombinant monoclonal antibody (AAA23836 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry) (Detection of human CD206/Mannose Receptor in FFPE MUTZ-3 cells by ICC. Antibody: Rabbit anti-CD206/Mannose Receptor recombinant monoclonal antibody (AAA23836 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

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CD206/Mannose Receptor, Monoclonal Recombinant Antibody (Cat# AAA23836)

Full Name

Rabbit anti-CD206/Mannose Receptor Recombinant Monoclonal Antibody [BLR109H]

Gene Names

MRC1; MMR; CD206; MRC1L1; CLEC13D; CLEC13DL; bA541I19.1

Reactivity

Human

Applications

Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry

Purity

Purified

Pricing

$295/0.01 mL $680/0.1 mL $980/5x0.01 mL $1,300/2x0.1 mL $1,890/3x0.1 mL $2,440/4x0.1 mL $2,755/5x0.1 mL

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IP (Immunoprecipitation) (Immunoprecipitation of SOX9 transfected lysate using SOX9 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with SOX9 rabbit polyclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SOX9 on HepG2 cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SOX9 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 0.7ug/ml].)

WB (Western Blot) (Western Blot analysis of SOX9 expression in transfected 293T cell line by SOX9 monoclonal antibody. Lane 1: SOX9 transfected lysate (56.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (SOX9 monoclonal antibody, Western Blot analysis of SOX9 expression in HepG2.)

WB (Western Blot) (Western Blot detection against Immunogen (38.21kD).)

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SOX9, Monoclonal Antibody (Cat# AAA24964)

Full Name

SOX9 (Transcription Factor SOX-9, SOX 9) (Biotin)

Gene Names

SOX9; CMD1; SRA1; CMPD1

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SNAI2 on HepG2 cell. [antibody concentration 10ug/ml].)

WB (Western Blot) (SNAI2 monoclonal antibody (M05). Western Blot analysis of SNAI2 expression in NIH/3T3.)

WB (Western Blot) (SNAI2 monoclonal antibody (M05). Western Blot analysis of SNAI2 expression in Raw 264.7.)

WB (Western Blot) (SNAI2 monoclonal antibody (M05), Western Blot analysis of SNAI2 expression in HepG2.)

WB (Western Blot) (SNAI2 monoclonal antibody. Western Blot analysis of SNAI2 expression in PC-12.)

WB (Western Blot) (SNAI2 monoclonal antibody. Western Blot analysis of SNAI2 expression in human liver.)

WB (Western Blot) (Western Blot detection against Immunogen (34.14kD).)

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SNAI2, Monoclonal Antibody (Cat# AAA24664)

Full Name

SNAI2 (Zinc Finger Protein SNAI2, Neural Crest Transcription Factor Slug, Protein Snail Homolog 2, SLUG, SLUGH, MGC10182) APC

Gene Names

SNAI2; SLUG; WS2D; SLUGH; SLUGH1; SNAIL2

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged SMARCB1 is ~0.03ng/ml as a capture antibody.)

IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to SMARCB1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml])

WB (Western Blot) (SMARCB1 monoclonal antibody. Western Blot analysis of SMARCB1 expression in NIH/3T3.)

WB (Western Blot) (SMARCB1 monoclonal antibody. Western Blot analysis of SMARCB1 expression in Raw 264.7.)

WB (Western Blot) (SMARCB1 monoclonal antibody, Western Blot analysis of SMARCB1 expression in Hela NE.)

WB (Western Blot) (SMARCB1 monoclonal antibody, Western Blot analysis of SMARCB1 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)

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SMARCB1, Monoclonal Antibody (Cat# AAA24365)

Full Name

SMARCB1 (SWI/SNF-related Matrix-associated Actin-dependent Regulator of Chromatin Subfamily B Member 1, BRG1-associated Factor 47, BAF47, Integrase Interactor 1 Protein, SNF5 Homolog, hSNF5, BAF47, INI1, SNF5L1) (AP)

Gene Names

SMARCB1; RDT; CSS3; INI1; SNF5; Snr1; BAF47; MRD15; RTPS1; Sfh1p; hSNFS; SNF5L1; SWNTS1; PPP1R144

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (F11R monoclonal antibody, Western Blot analysis of F11R expression in HepG2.)

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between PRKCZ and F11R. HeLa cells were stained with PRKCZ rabbit purified polyclonal 1:1200 and F11R mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Application Data (Detection limit for recombinant GST tagged F11R is ~0.1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to F11R on formalin-fixed paraffin-embedded human breast cancer tissue. [antibody concentration 1ug/ml].)

WB (Western Blot) (Western Blot analysis of F11R expression in transfected 293T cell line by F11R monoclonal antibody. Lane 1: F11R transfected lysate (32.6kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (59kD).)

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Junctional Adhesion Molecule 1, Monoclonal Antibody (Cat# AAA25728)

Full Name

Junctional Adhesion Molecule 1 (JAM-1, CD321, F11 Receptor, F11R, Junctional Adhesion Molecule A, JAMA, JCAM, KAT, Platelet Adhesion Molecule 1, PAM1, Platelet F11 Receptor, UNQ264/PRO301) (PE)

Gene Names

F11R; JAM; KAT; JAM1; JAMA; JCAM; CD321; PAM-1

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (EFHD1 monoclonal antibody (M05), Western Blot analysis of EFHD1 expression in HeLa.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to EFHD1 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3ug/ml].)

WB (Western Blot) (EFHD1 monoclonal antibody (M05). Western Blot analysis of EFHD1 expression in NIH/3T3.)

WB (Western Blot) (EFHD1 monoclonal antibody. Western Blot analysis of EFHD1 expression in Raw 264.7.)

WB (Western Blot) (EFHD1 monoclonal antibody. Western Blot analysis of EFHD1 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (33.81kD).)

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EFHD1, Monoclonal Antibody (Cat# AAA25378)

Full Name

EFHD1 (EF-hand Domain-containing Protein D1, EF-hand Domain-containing Protein 1, Swiprosin-2, SWS2, PP3051) (HRP)

Gene Names

EFHD1; SWS2; MST133; PP3051; MSTP133; FLJ13612; DKFZp781H0842

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to UBTF on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.5 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to UBTF on formalin-fixed paraffin-embedded human placenta. [antibody concentration 0.5 ug/ml])

Application Data (Detection limit for recombinant GST tagged UBTF is approximately 1ng/ml as a capture antibody.)

WB (Western Blot) (UBTF monoclonal antibody (M03), clone 1A2 Western Blot analysis of UBTF expression in HepG2.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to UBTF on HepG2 cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to UBTF on HepG2 cell. [antibody concentration 10 ug/ml])

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UBTF, Monoclonal Antibody (Cat# AAA25964)

Full Name

UBTF (Upstream Binding Transcription Factor, RNA Polymerase I, NOR-90, UBF) (AP)

Gene Names

UBTF; UBF; UBF1; UBF2; UBF-1; CONDBA; NOR-90

Applications

Immunohistochemistry, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD13: Alexa Fluor 647)

Application Data (Immunofluorescence staining of human tonsil cryosection with Mouse anti Human CD13 antibody, clone WM15 (red in A) and Mouse anti CD21 antibody, clone LB21 (green in B). The merged image is in C with nuclei counterstained blue using DAPI. High power.)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD13: APC)

Application Data (Immunofluorescence staining of human tonsil cryosection with Mouse anti Human CD13 antibody, clone WM15 (red in A) and Mouse anti CD21 antibody, clone LB21 (green in B). The merged image is in C with nuclei counterstained blue using DAPI. Medium power.)

Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD13)

Application Data (Immunofluorescence staining of human tonsil cryosection with Mouse anti Human CD13 antibody, clone WM15 (red in A) and Mouse anti CD21 antibody, clone LB21 (green in B). The merged image is in C with nuclei counterstained blue using DAPI. Low power.)

Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD13 antibody, clone WM15 followed by Histar Detection System. Medium power)

Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD13:RPE)

Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD13:FITC)

Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD13 antibody, clone WM15 followed by Histar Detection System. Medium power)

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CD13, Monoclonal Antibody (Cat# AAA12210)

Full Name

MOUSE ANTI HUMAN CD13:Low Endotoxin

Gene Names

ANPEP; APN; CD13; LAP1; P150; PEPN; GP150

Applications

Immunohistochemistry, Flow Cytometry, Functional Assay, Immunoprecipitation

Pricing

$915/0.5 mg $1,755/2x0.5 mg $2,550/3x0.5 mg $3,300/4x0.5 mg $3,790/5x0.5 mg

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WB (Western Blot) (CDK2 monoclonal antibody. Western Blot analysis of CDK2 expression in Jurkat.)

WB (Western Blot) (Western blot analysis of CDK2 over-expressed 293 cell line, cotransfected with CDK2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CDK2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged CDK2 is ~0.03ng/ml as a capture antibody.)

WB (Western Blot) (Western Blot analysis of CDK2 expression in transfected 293T cell line by CDK2 monoclonal antibody. Lane 1: CDK2 transfected lysate (33.9kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (CDK2 monoclonal antibody, Western Blot analysis of CDK2 expression in HL-60.)

WB (Western Blot) (Western Blot detection against Immunogen (35.42kD).)

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CDK2, Monoclonal Antibody (Cat# AAA24165)

Full Name

CDK2 (Cell Division Protein Kinase 2, p33 Protein Kinase) (AP)

Gene Names

CDK2; CDKN2; p33(CDK2)

Reactivity

Human

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data

Application Data

Application Data (Detection limit for 123091 is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence to AHR on HeLa cell using 123091 (10ug/ml).)

IP (Immunoprecipitation) (Immunoprecipitation of AHR transfected lysate using 123091 and Protein A Magnetic Bead and immunoblotted with AHR rabbit polyclonal antibody.)

WB (Western Blot) (Western Blot analysis of AHR over-expressed 293 cell line, cotransfected with AHR Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with 123091. GAPDH (36.1kD) used as specificity and loading control.)

WB (Western Blot) (Western Blot detection against immunogen (36.74kD).)

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AHR, Monoclonal Antibody (Cat# AAA24424)

Full Name

AHR (Aryl Hydrocarbon Receptor, Ah Receptor, AhR, Class E Basic Helix-loop-helix Protein 76, bHLHe76, BHLHE76) (APC)

Gene Names

AHR; bHLHe76

Reactivity

Human

Applications

Immunofluorescence, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (DAAM1 monoclonal antibody Western Blot analysis of DAAM1 expression in A-431.)

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between RHOA and DAAM1 HeLa cells were stained with RHOA rabbit purified polyclonal 1:1200 and DAAM1 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Application Data (Detection limit for recombinant GST tagged DAAM1 is ~1ng/ml as a capture antibody.)

WB (Western Blot) (Western Blot analysis of DAAM1 expression in transfected 293T cell line by DAAM1 monoclonal antibody. Lane 1: DAAM1 transfected lysate (122.3kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (DAAM1 monoclonal antibody Western Blot analysis of DAAM1 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (38.21kD).)

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DAAM1, Monoclonal Antibody (Cat# AAA25366)

Full Name

DAAM1 (Disheveled-associated Activator Of Morphogenesis 1, KIAA0666) (HRP)

Reactivity

Human, Rat

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (HHIP monoclonal antibody. Western Blot analysis of HHIP expression in MCF-7.)

WB (Western Blot) (HHIP monoclonal antibody, Western Blot analysis of HHIP expression in Hela NE.)

Application Data (Detection limit for recombinant GST tagged HHIP is ~1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of HHIP transfected lysate using HHIP monoclonal antibody and Protein A Magnetic Bead and immunoblotted with HHIP rabbit polyclonal antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HHIP on formalin-fixed paraffin-embedded human heart. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of HHIP expression in transfected 293T cell line by HHIP monoclonal antibody. Lane 1: HHIP transfected lysate (78.9kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)

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HHIP, Monoclonal Antibody (Cat# AAA25713)

Full Name

HHIP (Hedgehog-interacting Protein, HHIP, HIP, UNQ5825/PRO19644, FLJ20992, FLJ90230) (PE)

Gene Names

HHIP; HIP

Reactivity

Human

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (RNF2 monoclonal antibody (M03), clone 3G6. Western Blot analysis of RNF2 expression in Raw 264.7.)

WB (Western Blot) (RNF2 monoclonal antibody (M03), clone 3G6. Western Blot analysis of RNF2 expression in PC-12.)

WB (Western Blot) (RNF2 monoclonal antibody (M03), clone 3G6. Western Blot analysis of RNF2 expression in NIH/3T3.)

Application Data (Detection limit for recombinant GST tagged RNF2 is approximately 1ng/ml as a capture antibody.)

WB (Western Blot) (RNF2 monoclonal antibody (M03), clone 3G6. Western Blot analysis of RNF2 expression in human stomach.)

WB (Western Blot) (RNF2 monoclonal antibody (M03), clone 3G6. Western Blot analysis of RNF2 expression in Hela S3 NE (Cat # L013V3).)

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RNF2, Monoclonal Antibody (Cat# AAA26521)

Full Name

RNF2 (RING Finger Protein 2, BAP-1, BAP1, DING, HIPI3, Ring1B, Ring2) (HRP)

Gene Names

RNF2; BAP1; DING; BAP-1; HIPI3; RING2; RING1B

Applications

Immunofluorescence, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with CFHR5 antibody at 1/100 dilution (green) compared with an unlabelled control (cells without incubation with primary antibody; red).)

ICC (Immunocytochemistry) (ICC staining CFHR5 (green) and Actin filaments (red) in Hela cells. The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human ovarian cancer tissue using anti-CFHR5 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human cervical cancer tissue using anti-CFHR5 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of CFHR5 on different cell lysate using anti-CFHR5 antibody at 1/1, 000 dilution. Positive control�� Lane1: HepG2 Lane2: K562 Lane3: L-02 Lane4: SK-Hep-1 Lane5: SMMC-7721 Lane6: NIH/3T3)

WB (Western Blot) (Western blot analysis of CFHR5 on HEK293 (1) and CFHR5-hIgGFc transfected HEK293 (2) cell lysate using anti-CFHR5 antibody at 1/1, 000 dilution.)

WB (Western Blot) (Western blot analysis of CFHR5 on human CFHR5 recombinant protein using anti-CFHR5 antibody at 1/1, 000 dilution.)

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CFHR5, Monoclonal Antibody (Cat# AAA29902)

Full Name

CFHR5 Antibody

Gene Names

CFHR5; FHR5; CFHL5; FHR-5; CFHR5D

Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,015/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of Jurkat cells with Caspase-2 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining Caspase-2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Caspase-2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Caspase-2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Caspase-2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Caspase-2 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Caspase-2 on 293 cell lysate using anti-Caspase-2 antibody at 1/1, 000 dilution.)

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Caspase-2, Monoclonal Antibody (Cat# AAA29993)

Full Name

Caspase-2 Antibody

Gene Names

CASP2; ICH1; NEDD2; CASP-2; NEDD-2; PPP1R57

Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with ATP citrate lyase antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining ATP citrate lyase in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining ATP citrate lyase in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining ATP citrate lyase in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-ATP citrate lyase antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse thyroid tissue using anti-ATP citrate lyase antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-ATP citrate lyase antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-ATP citrate lyase citrate lyase antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of ATP citrate lyase on different lysates using anti-ATP citrate lyase antibody at 1/1, 000 dilution. Positive control: Lane 1: Mouse kidney Lane 2: Mouse colon Lane 3: MCF-7)

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ATP, Monoclonal Antibody (Cat# AAA30094)

Full Name

ATP Citrate Lyase Antibody

Gene Names

ACLY; ACL; ATPCL; CLATP

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of BT-20 cells using MCL1 antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using MCL1 antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of HepG2 cells using MCL1 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human kidney using MCL1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human breast using MCL1 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human pancreas using MCL1 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using MCL1 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

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MCL1, Monoclonal Antibody (Cat# AAA28233)

Full Name

MCL1 Monoclonal Antibody

Gene Names

MCL1; TM; EAT; MCL1L; MCL1S; Mcl-1; BCL2L3; MCL1-ES; bcl2-L-3; mcl1/EAT

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunoprecipitation

Purity

Affinity Purification

Pricing

$285/0.02 mL $465/0.1 mL $885/2x0.1 mL $1,275/3x0.1 mL $1,650/4x0.1 mL $1,765/5x0.1 mL

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ICC (Immunocytochemistry) (ICC staining Histone H4 (acetyl K5) in SH-SY-5Y cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Histone H4 (acetyl K5) in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Histone H4 (acetyl K5) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Histone H4 (acetyl K5) antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Histone H4 (acetyl K5) antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Histone H4 (acetyl K5) antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Histone H4 (acetyl K5) antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H4 (acetyl K5) antibody. Counter stained with hematoxylin.)

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Histone H4, Monoclonal Antibody (Cat# AAA30536)

Full Name

Histone H4 (Acetyl K5) Antibody

Gene Names

HIST2H4B; H4/o

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation

Purity

ProA affinity purified

Pricing

$390/0.05 mL $510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,015/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of Jurkat cells with NFAT2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)

ICC (Immunocytochemistry) (ICC staining NFAT2 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining NFAT2 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining NFAT2 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human skeleton muscle tissue using anti-NFAT2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-NFAT2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NFAT2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human thymus tissue using anti-NFAT2 antibody. Counter stained with hematoxylin.)

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NFAT2, Monoclonal Antibody (Cat# AAA30348)

Full Name

NFAT2 Antibody

Gene Names

NFATC1; NFAT2; NFATc; NF-ATC

Reactivity

Human

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with BMI1 antibody at 1/100 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining Bmi1 in Hela cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Bmi1 in Lovo cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Bmi1 in A549 cells (red). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-BMI1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Bmi1 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Bmi1 on different lysates using anti-Bmi1 antibody at 1/1, 000 dilution. Positive control:   Lane 1: 293T             Lane 2: Jurkat Lane 3: Hela              Lane 4: MCF-7 Lane 5: HepG2           Lane 6: NIH/3T3 Lane 7: PC12             Lane 8: Mouse kidney Lane 9: Human kidney     Lane 10: K562 Lane 11: Human brain)

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BMI1, Monoclonal Antibody (Cat# AAA29913)

Full Name

BMI1 Antibody

Gene Names

BMI1; PCGF4; RNF51; FLVI2/BMI1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,015/5x0.1 mL

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Application Data

Application Data

Application Data

Application Data

Application Data

Application Data

Application Data

Application Data

SDS-PAGE (Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Her-2(ABT077) antibody. The HRP-conjugated Goat anti-M)

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Her-2, Monoclonal Antibody (Cat# AAA30899)

Full Name

Her-2 (ABT077) Antibody

Gene Names

ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu

Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Immunofluorescence

Purity

Protein G

Pricing

$240/0.02 mL $315/0.05 mL $375/0.1 mL $600/0.2 mL $1,140/2x0.2 mL $1,650/3x0.2 mL $2,135/4x0.2 mL $2,350/5x0.2 mL

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WB (Western Blot) (NEK10 monoclonal antibody, Western Blot analysis of NEK10 expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (36.01kD).)

Application Data (Detection limit for recombinant GST tagged NEK10 is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NEK10 on HeLa cell. [antibody concentration 10ug/ml].)

WB (Western Blot) (Western Blot analysis of NEK10 expression in transfected 293T cell line by NEK10 monoclonal antibody. Lane 1: NEK10 transfected lysate (53.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (NEK10 monoclonal antibody. Western Blot analysis of NEK10 expression in NIH/3T3.)

WB (Western Blot) (NEK10 monoclonal antibody. Western Blot analysis of NEK10 expression in Raw 264.7.)

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NEK10, Monoclonal Antibody (Cat# AAA24583)

Full Name

NEK10 (Never in Mitosis A-related Kinase 10, NimA-related Protein Kinase 10, FLJ32685, Serine/Threonine Protein Kinase Nek10, FLJ32685) APC

Reactivity

Human, Mouse

Applications

Immunofluorescence, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged PLK1 is ~1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of PLK1 transfected lysate using anti-PLK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PLK1 rabbit polyclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PLK1 on HeLa cell. [antibody concentration 10ug/ml])

WB (Western Blot) (Western Blot analysis of PLK1 expression in transfected 293T cell line by PLK1 monoclonal antibody. Lane 1: PLK1 transfected lysate (68.3kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western blot analysis of PLK1 in extracts from 293T, HeLa and A549 cell using PLK1 monoclonal antibody.)

WB (Western Blot) (PLK1 monoclonal antibody Western Blot analysis of PLK1 expression in Hela NE.)

WB (Western Blot) (Western Blot detection against Immunogen (91.85kD).)

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PLK1, Monoclonal Antibody (Cat# AAA25209)

Full Name

PLK1 (STPK13, Polo-like Kinase 1, Serine/Threonine-protein Kinase 13, Serine/Threonine-protein Kinase PLK1, PLK, PLK-1) (FITC)

Gene Names

PLK1; PLK; STPK13

Reactivity

Human

Applications

Immunofluorescence, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged EIF2AK2 is approximately 0.03ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to EIF2AK2 on formalin-fixed paraffin-embedded human adrenal gland. [antibody concentration 1.5 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to EIF2AK2 on formalin-fixed paraffin-embedded human adrenal gland. [antibody concentration 1.5 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to EIF2AK2 on HeLa cell. [antibody concentration 30 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to EIF2AK2 on HeLa cell. [antibody concentration 30 ug/ml])

WB (Western Blot) (EIF2AK2 monoclonal antibody (M02), clone 1D11 Western Blot analysis of EIF2AK2 expression in K-562.)

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EIF2AK2, Monoclonal Antibody (Cat# AAA25929)

Full Name

EIF2AK2 (Eukaryotic Translation Initiation Factor 2-alpha Kinase 2, EIF2AK1, MGC126524, PKR, PRKR) (AP)

Gene Names

EIF2AK2; PKR; PRKR; EIF2AK1; PPP1R83

Applications

Immunohistochemistry, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (GNRHR2 monoclonal antibody, Western Blot analysis of GNRHR2 expression in HeLa.)

Application Data (Detection limit for recombinant GST tagged GNRHR2 is ~0.03ng/ml as a capture antibody.)

WB (Western Blot) (GNRHR2 monoclonal antibody. Western Blot analysis of GNRHR2 expression in NIH/3T3.)

WB (Western Blot) (GNRHR2 monoclonal antibody. Western Blot analysis of GNRHR2 expression in Raw 264.7.)

WB (Western Blot) (GNRHR2 monoclonal antibody. Western Blot analysis of GNRHR2 expression in HepG2.)

WB (Western Blot) (Western Blot detection against Immunogen (32.27kD).)

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GNRHR2, Monoclonal Antibody (Cat# AAA25703)

Full Name

GNRHR2 (Putative Gonadotropin-releasing Hormone II Receptor, GnRH II Receptor, GnRH-II-R, Type II GnRH Receptor) (PE)

Gene Names

FZD2; Fz2; fz-2; fzE2; hFz2; OMOD2

Reactivity

Human, Mouse

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged CHUK is 0.03 ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CHUK on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CHUK on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (CHUK monoclonal antibody (M03), clone 4B8 Western Blot analysis of CHUK expression in HeLa (Cat # L013V1).)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CHUK on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to CHUK on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml])

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CHUK, Monoclonal Antibody (Cat# AAA26486)

Full Name

CHUK (conserved Helix-loop-Helix ubiquitous Kinase, IKBKA, IKK-alpha, IKK1, IKKA, NFKBIKA, TCF16) (HRP)

Gene Names

CHUK; IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of Jurkat cells with HuR/ELAVL1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-HuR/ELAVL1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-HuR/ELAVL1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-HuR/ELAVL1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-HuR/ELAVL1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat lung tissue using anti-HuR/ELAVL1 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of HuR/ELAVL1 on different lysates using anti-HuR/ELAVL1 antibody at 1/1, 000 dilution. POSITIVE CONTROL: Lane 1: Rat spleen tissue Lane 2: Rat heart tisse Lane 3: PC-12 Lane 4: Mouse heart tissue Lane 5: Hela)

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HuR ELAVL1, Monoclonal Antibody (Cat# AAA30382)

Full Name

HuR ELAVL1 Antibody

Gene Names

ELAVL1; HUR; Hua; MelG; ELAV1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunoprecipitation, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of NIH/3T3 cells with STAT5A+B antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining STAT5A+B in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining STAT5A+B in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining STAT5A+B in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-STAT5A+B antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-STAT5A+B antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-STAT5A+B antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of STAT5A+B on different lysates using anti-STAT5A+B antibody at 1/1, 000 dilution. Positive control: Lane 1: K562 Lane 2: Jurkat Lane 3: PC-12)

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STAT 5A+B, Monoclonal Antibody (Cat# AAA30230)

Full Name

STAT 5A+B Antibody

Gene Names

STAT5A; MGF; STAT5

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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WB (Western Blot) (ACBD3 monoclonal antibody. Western Blot analysis of ACBD3 expression in PC-12.)

WB (Western Blot) (ACBD3 monoclonal antibody, Western Blot analysis of ACBD3 expression in HeLa.)

Application Data (Detection limit for recombinant GST tagged ACBD3 is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ACBD3 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ACBD3 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of ACBD3 expression in transfected 293T cell line by ACBD3 monoclonal antibody. Lane 1: ACBD3 transfected lysate (60.6kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (37kD).)

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ACBD3, Monoclonal Antibody (Cat# AAA25004)

Full Name

ACBD3 (Golgi Resident Protein GCP60, Acyl-CoA-binding Domain-containing Protein 3, Golgi Complex-associated Protein 1, GOCAP1, Golgi Phosphoprotein 1, GOLPH1, PBR- and PKA-associated Protein 7, Peripheral Benzodiazepine Receptor-associated Protein PAP7, G

Gene Names

ACBD3; PAP7; GCP60; GOCAP1; GOLPH1

Reactivity

Human, Mouse, Rat

Applications

EIA, IF, IHC, WB

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD20: Pacific Blue (AAA11937PB))

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD20:Alexa Fluor 488 (AAA11937A488))

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD20:Biotin)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD20)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD20:Alexa Fluor 647 (AAA11937A647))

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD20:FITC)

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CD20, Monoclonal Antibody (Cat# AAA11937)

Full Name

MOUSE ANTI HUMAN CD20

Gene Names

MS4A1; B1; S7; Bp35; CD20; CVID5; MS4A2; LEU-16

Reactivity

Rhesus Monkey

Applications

Immunohistochemistry, Flow Cytometry

Pricing

$600/0.2 mg $1,140/2x0.2 mg $1,650/3x0.2 mg $2,135/4x0.2 mg $2,385/5x0.2 mg

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FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of U2OS cells using anti-Sorbitol Dehydrogenase/SORD antibody (AAA19716).Overlay histogram showing U2OS cells stained with AAA19716 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Sorbitol Dehydrogenase/SORD Antibody (AAA19716, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of Sorbitol Dehydrogenase/SORD using anti-Sorbitol Dehydrogenase/SORD antibody (AAA19716).Sorbitol Dehydrogenase/SORD was detected in a paraffin-embedded section of rat kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Sorbitol Dehydrogenase/SORD Antibody (AAA19716) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of Sorbitol Dehydrogenase/SORD using anti-Sorbitol Dehydrogenase/SORD antibody (AAA19716).Sorbitol Dehydrogenase/SORD was detected in a paraffin-embedded section of human SM fatty carcinoma of the left kidney tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Sorbitol Dehydrogenase/SORD Antibody (AAA19716) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of Sorbitol Dehydrogenase/SORD using anti-Sorbitol Dehydrogenase/SORD antibody (AAA19716).Sorbitol Dehydrogenase/SORD was detected in a paraffin-embedded section of human renal clear cell carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Sorbitol Dehydrogenase/SORD Antibody (AAA19716) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of Sorbitol Dehydrogenase/SORD using anti-Sorbitol Dehydrogenase/SORD antibody (AAA19716).Sorbitol Dehydrogenase/SORD was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Sorbitol Dehydrogenase/SORD Antibody (AAA19716) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Mouse IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of Sorbitol Dehydrogenase/SORD using anti-Sorbitol Dehydrogenase/SORD antibody (AAA19716).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human HepG2 whole cell lysates,Lane 3: rat liver tissue tissue lysates,Lane 4: rat kidney tissue lysates,Lane 5: mouse liver tissue lysates,Lane 6: mouse kidney tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Sorbitol Dehydrogenase/SORD antigen affinity purified monoclonal antibody (#AAA19716) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Sorbitol Dehydrogenase/SORD at approximately 40 kDa. The expected band size for Sorbitol Dehydrogenase/SORD is at 40 kDa.)

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Sorbitol Dehydrogenase/SORD, Monoclonal Antibody (Cat# AAA19716)

Full Name

Anti-Sorbitol Dehydrogenase/SORD Antibody Picoband (monoclonal, 12B10G2)

Gene Names

SORD; SORD1; HEL-S-95n

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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IF (Immunofluorescence) (Figure 7. IF analysis of Cytokeratin 5 using anti-Cytokeratin 5 antibody (AAA19665).Cytokeratin 5 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 5 ug/mL mouse anti-Cytokeratin 5 Antibody (AAA19665) overnight at 4 degree C. Biotin conjugated goat anti-mouse IgG (BA1001) was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using DyLight488 Conjugated Avidin (BA1128). The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

FCM (Flow Cytometry) (Figure 6. Flow Cytometry analysis of A431 cells using anti-Cytokeratin 5 antibody (AAA19665).Overlay histogram showing A431 cells stained with AAA19665 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with mouse anti-Cytokeratin 5 Antibody (AAA19665, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-mouse IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was mouse IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 5. IF analysis of Cytokeratin 5 using anti-Cytokeratin 5 antibody (AAA19665).Cytokeratin 5 was detected in an immunocytochemical section of A431 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent (AR0022) for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5 ug/mL mouse anti-Cytokeratin 5 Antibody (AAA19665) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Mouse IgG was used as secondary antibody at 1:100 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of Cytokeratin 5 using anti-Cytokeratin 5 antibody (AAA19665).Cytokeratin 5 was detected in a paraffin-embedded section of human metaplasia of squamous cells of the renal pelvis tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Cytokeratin 5 Antibody (AAA19665) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of Cytokeratin 5 using anti-Cytokeratin 5 antibody (AAA19665).Cytokeratin 5 was detected in a paraffin-embedded section of human rectal moderately differentiated adenocarcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Cytokeratin 5 Antibody (AAA19665) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of Cytokeratin 5 using anti-Cytokeratin 5 antibody (AAA19665).Cytokeratin 5 was detected in a paraffin-embedded section of human lung cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-Cytokeratin 5 Antibody (AAA19665) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of Cytokeratin 5 using anti-Cytokeratin 5 antibody (AAA19665).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human A431 whole cell lysates,Lane 2: human SH-SY5Y whole cell lysates,Lane 3: human Hacat whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-Cytokeratin 5 antigen affinity purified monoclonal antibody (#AAA19665) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Cytokeratin 5 at approximately 62 kDa. The expected band size for Cytokeratin 5 is at 62 kDa.)

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Cytokeratin 5, Monoclonal Antibody (Cat# AAA19665)

Full Name

Anti-Cytokeratin 5 Antibody Picoband (monoclonal, 5D3F7)

Gene Names

KRT5; K5; CK5; DDD; EBS2; KRT5A

Reactivity

Human

Applications

WB, IHC, ICC, IF, FC/FACS

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)

Application Data (Published customer image: Post-injury 7,8-dihydroxyflavone treatment increased brain BDNF levels and promoted CREB activation. (A) Bar graphs demonstrating brain derived neurotrophic factor (BDNF) protein concentrations measured by enzyme-linked immunosorbent assay (ELISA) in sham-injured, vehicle-treated, and 20 mg/kg 7,8-dihydroxyflavone (DHF 20)-treated mice at 4 days post-TBI. DHF 20 significantly increased BDNF protein levels in the ipsilateral hemisphere at 4 days post-injury. (B) Bar graphs demonstrating BDNF mRNA expression measured by real -time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) at 1 and 4 days post-injury. DHF 20 significantly increased BDNF mRNA levels in the ipsilateral hemisphere at 4 days post-injury. (C) Western blot analysis of the phospho-CREB level in the ipsilateral hemisphere of sham-injured, vehicle-treated, and DHF 20-treated mice at 1 h, 1 day, and 4 days post-injury. DHF 20 enhanced CREB phosphorylation at 4 days post-injury. (D) Identification of phospho-CREB-positive cells 4 day post-injury in the peri-contusional margin by double immunofluorescence staining. Phospho-CREB is shown in red, and NeuN (neurons), GFAP (astrocytes), or F4/80 (microglia) is shown in green. Co-localization of phospho-CREB with NeuN is shown by yellow labeling. Sections were stained with DAPI (blue) to show all nuclei. Values are mean +/- SEM; *P)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: Pacific Blue)

Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen:Biotin)

Application Data (Frozen mouse spleen stained with A: Rat anti Mouse F4/80 followed by Goat anti Rat IgG:HRP or B: Rat anti Mouse F4/80 preincubated with 2 molar excess of Human anti Idiotypic (HCA154) followed by Goat anti Rat IgG:HRP)

Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen:Low Endotoxin)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: RPE - Alexa Fluor 750)

Application Data (Staining of mouse peritoneal macrophages cells with Rat anti Mouse F4/80 antigen:APC)

Application Data (Immunofluorescence image of mouse small intestine stained with Rat anti Mouse F4/80 , red and Rat anti Mouse CD45 , green; nuclei are stained with DAPI, blue. The image higlights macrophages within the lamina propria)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen:FITC)

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F4/80, Monoclonal Antibody (Cat# AAA12168)

Full Name

RAT ANTI MOUSE F4/80:FITC

Gene Names

Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7

Applications

Flow Cytometry, Immunofluorescence

Pricing

$830/0.5 mg $1,590/2x0.5 mg $2,305/3x0.5 mg $2,980/4x0.5 mg $3,415/5x0.5 mg

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Application Data (Detection limit for recombinant GST tagged PCNA is ~0.1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of PCNA transfected lysate using PCNA monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PCNA rabbit polyclonal antibody)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PCNA on HeLa cell. [antibody concentration 10ug/ml])

WB (Western Blot) (Western Blot analysis of PCNA expression in transfected 293T cell line by PCNA monoclonal antibody Lane 1: PCNA transfected lysate (28.8kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (PCNA monoclonal antibody Western Blot analysis of PCNA expression in Hela.)

WB (Western Blot) (Western Blot detection against Immunogen (54.45kD).)

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PCNA, Monoclonal Antibody (Cat# AAA24896)

Full Name

PCNA (Proliferating Cell Nuclear Antigen, Cyclin, MGC8367) (Biotin)

Gene Names

PCNA; ATLD2

Reactivity

Human

Applications

Immunofluorescence, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (FGR monoclonal antibody (M01), clone 3G10. Western Blot analysis of FGR expression in Raw 264.7.)

WB (Western Blot) (FGR monoclonal antibody (M01), clone 3G10. Western Blot analysis of FGR expression in PC-12.)

Application Data (Detection limit for recombinant GST tagged FGR is approximately 0.3ng/ml as a capture antibody.)

WB (Western Blot) (FGR monoclonal antibody (M01), clone 3G10 Western Blot analysis of FGR expression in HeLa.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FGR on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to FGR on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 3 ug/ml])

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FGR, Monoclonal Antibody (Cat# AAA26158)

Full Name

FGR (Gardner-Rasheed feline Sarcoma Viral (v-fgr) Oncogene homolog, FLJ43153, MGC75096, SRC2, c-fgr, c-src2, p55c-fgr, p58c-fgr) (Biotin)

Gene Names

FGR; SRC2; c-fgr; c-src2; p55-Fgr; p58-Fgr; p55c-fgr; p58c-fgr

Applications

Immunohistochemistry, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged HDAC1 is approximately 1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HDAC1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 0.7 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HDAC1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 0.7 ug/ml])

WB (Western Blot) (HDAC1 monoclonal antibody (M11), clone 5A11 Western Blot analysis of HDAC1 expression in Hela S3 NE (Cat # L013V3).)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HDAC1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HDAC1 on HeLa cell. [antibody concentration 10 ug/ml])

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HDAC1, Monoclonal Antibody (Cat# AAA26453)

Full Name

HDAC1 (Histone Deacetylase 1, DKFZp686H12203, GON-10, HD1, RPD3, RPD3L1) (HRP)

Gene Names

HDAC1; HD1; RPD3; KDAC1; GON-10; RPD3L1

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of ALB expression in transfected 293T cell line by ALB monoclonal antibody. Lane 1: ALB transfected lysate (69.4kD). Lane 2: Non-transfected lysate.)

Application Data (Detection limit for recombinant GST tagged ALB is ~1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of ALB transfected lysate using ALB monoclonal antibody and Protein A Magnetic Bead and immunoblotted with ALB rabbit polyclonal antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ALB on formalin-fixed paraffin-embedded human hepatocellular carcinoma tissue. [antibody concentration 3ug/ml].)

WB (Western Blot) (ALB monoclonal antibody Western Blot analysis of ALB expression in HepG2.)

WB (Western Blot) (Western Blot detection against Immunogen (90.75kD).)

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ALB, Monoclonal Antibody (Cat# AAA24430)

Full Name

ALB (Albumin, Serum Albumin, GIG20, GIG42, PRO0903, PRO1708, PRO2044, PRO2619, PRO2675, UNQ696/PRO1341) APC

Gene Names

ALB; HSA; PRO0883; PRO0903; PRO1341

Reactivity

Human

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (SP1 monoclonal antibody (M01), clone 4H6. Western Blot analysis of SP1 expression in Hela S3 NE.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 1 ~ 10 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SP1 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 1 ~ 10 ug/ml])

WB (Western Blot) (SP1 monoclonal antibody (M01), clone 4H6 Western Blot analysis of SP1 expression in IMR-32.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SP1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SP1 on HeLa cell. [antibody concentration 10 ug/ml])

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SP1, Monoclonal Antibody (Cat# AAA26207)

Full Name

SP1 (Sp1 Transcription Factor) (Biotin)

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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ICC (Immunocytochemistry) (ICC staining CD9 in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining CD9 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-CD9 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-CD9 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-CD9 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD9 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD9 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of CD9 on different lysates using anti-CD9 antibody at 1/500 dilution. Positive control: Lane 1: Mouse heart Lane 2: Jurkat)

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CD9, Monoclonal Antibody (Cat# AAA29982)

Full Name

CD9 Antibody

Gene Names

CD9; MIC3; MRP-1; BTCC-1; DRAP-27; TSPAN29; TSPAN-29

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Bcl-XL antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining Bcl-XL in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Bcl-XL in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Bcl-XL in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Bcl-XL antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Bcl-XL antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Bcl-XL antibody. Counter stained with hematoxylin.)

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BCL-XL, Monoclonal Antibody (Cat# AAA30008)

Full Name

BCL-XL Antibody

Gene Names

BCL2L1; BCLX; BCL2L; BCLXL; BCLXS; Bcl-X; bcl-xL; bcl-xS; PPP1R52; BCL-XL/S

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Met antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining Met in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Met antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Met antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Met antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Met antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Met on different lysates using anti-Met antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: HepG2)

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MET, Monoclonal Antibody (Cat# AAA30043)

Full Name

MET (C-Met) Antibody

Gene Names

MET; HGFR; AUTS9; RCCP2; c-Met

Reactivity

Human

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Smad3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining Smad3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Smad3 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Smad3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Smad3 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Smad3 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Smad3 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Smad3 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Smad3 on A549 cell lysates using anti-Smad3 antibody at 1/1, 000 dilution.)

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SMAD3, Monoclonal Antibody (Cat# AAA30057)

Full Name

SMAD3 Antibody

Gene Names

SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: IHC 1:200 IF 1:50-200)

IHC (Immunohistchemistry) (Dilution: IHC 1:200 IF 1:50-200)

IHC (Immunohistochemistry) (Dilution: IHC 1:200 IF 1:50-200)

IF (Immunofluorescence) (Dilution: IHC 1:200 IF 1:50-200)

Application Data (Dilution: IHC 1:200 IF 1:50-200)

IHC (Immunohistochemistry-Paraffin) (Dilution: IHC 1:200 IF 1:50-200)

IHC (Immunohistochemistry) (Dilution: IHC: 1:200 IF 1:50-200)

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CD68, Monoclonal Antibody (Cat# AAA28861)

Full Name

CD68 Monoclonal Antibody (6F3)

Gene Names

CD68; GP110; LAMP4; SCARD1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunofluorescence

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

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ICC (Immunocytochemistry) (ICC staining Syndecan 1 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Syndecan 1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Syndecan 1 in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Syndecan 1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Syndecan 1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Syndecan 1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Syndecan 1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Syndecan 1 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Syndecan 1 on NIH-3T3 cells lysates using anti-Syndecan 1 antibody at 1/500 dilution.)

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Syndecan 1, Monoclonal Antibody (Cat# AAA30318)

Full Name

Syndecan 1 Antibody

Gene Names

SDC1; SDC; CD138; SYND1; syndecan

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Rat testis using GST3/GSTP1 Rabbit mAb (AAA28477) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat liver using GST3/GSTP1 Rabbit mAb (AAA28477) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse brain using GST3/GSTP1 Rabbit mAb (AAA28477) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Human placenta using GST3/GSTP1 Rabbit mAb (AAA28477) at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human lung squamous carcinoma tissue using GST3/GSTP1 Rabbit mAb (AAA28477) at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma using GST3/GSTP1 Rabbit mAb (AAA28477) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human liver using GST3/GSTP1 Rabbit mAb (AAA28477) at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human breast cancer using GST3/GSTP1 Rabbit mAb (AAA28477) at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

WB (Western Blot) (Western blot analysis of various lysates using GST3/GSTP1 Rabbit mAb (AAA28477) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 3min.)

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GST3/GSTP1, Monoclonal Antibody (Cat# AAA28477)

Full Name

GST3/GSTP1 Rabbit mAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Purity

Affinity purification

Pricing

$300/0.02 mL $500/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,780/4x0.1 mL $1,960/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of K562 cells with Leptin Receptor antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)

ICC (Immunocytochemistry) (ICC staining Leptin Receptor in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Leptin Receptor antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human prostate tissue using anti-Leptin Receptor antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Leptin Receptor antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Leptin Receptor antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat heart tissue using anti-Leptin Receptor antibody. Counter stained with hematoxylin.)

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Leptin, Monoclonal Antibody (Cat# AAA30347)

Full Name

Leptin Receptor Antibody

Gene Names

LEPR; OBR; OB-R; CD295; LEP-R; LEPRD

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of Jurkat cells with gamma Tubulin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining gamma Tubulin in SHG-44 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining gamma Tubulin in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining gamma Tubulin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-gamma Tubulin antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-gamma Tubulin antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of gamma Tubulin on different lysates using anti-gamma Tubulin antibody at 1/1, 000 dilution. Positive control: Lane 1: A431 Lane 2: Jurkat Lane 3: Mouse brain)

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gamma Tubulin, Monoclonal Antibody (Cat# AAA30272)

Full Name

gamma Tubulin Antibody

Gene Names

TUBG1; TUBG; GCP-1; TUBGCP1

Reactivity

Human, Mouse, Rat, Monkey, Chicken, Hamster, Cow, Dog, Fish, Xenopus tropicalis

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

What are Monoclonal Antibodies?

Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.

This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.

AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.

What Are The Uses of Monoclonal Antibodies

Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.

ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.

Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.

Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.

Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.

How Monoclonal Antibodies Are Used as Medicine?

Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).

Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:

• Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.


• Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.


• Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.

Why Buy Monoclonal Antibodies From Us?

At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.

1. Validated for Versatile Applications

The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).

2. Wide Selection & Specialized Options

We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.

3. High-Quality Proteins

Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.

Frequently Asked Questions

1. Are your monoclonal antibodies validated for specific applications?

Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.

2. How do I choose the right monoclonal antibody for my application?

Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.

3. How quickly can I receive my order?

Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.