Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.
WB (Western Blot) (MAN1B1 monoclonal antibody Western Blot analysis of MAN1B1 expression in LNCaP.)
Application Data (Detection limit for recombinant GST tagged MAN1B1 is ~0.03ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to MAN1B1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3ug/ml])
WB (Western Blot) (MAN1B1 monoclonal antibody Western Blot analysis of MAN1B1 expression in A-431.)
WB (Western Blot) (MAN1B1 monoclonal antibody Western Blot analysis of MAN1B1 expression in HepG2.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between BAD and MAPK8 Mahlavu cells were stained with BAD rabbit purified polyclonal 1:1200 and anti-MAPK8 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between PXN and MAPK8 Huh7 cells were stained with PXN rabbit purified polyclonal 1:1200 and MAPK8 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between CRK and MAPK8 HeLa cells were stained with CRK rabbit purified polyclonal 1:1200 and MAPK8 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
WB (Western Blot) (Western blot analysis of MAPK8 over-expressed 293 cell line, cotransfected with MAPK8 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MAPK8 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged MAPK8 is 1ng/ml as a capture antibody.)
WB (Western Blot) (Western Blot analysis of MAPK8 expression in transfected 293T cell line by MAPK8 monoclonal antibody. Lane 1: MAPK8 transfected lysate (48.3kD). Lane 2: Non-transfected lysate.)
Application Data (Detection limit for recombinant GST tagged CSE1L is approximately 0.1ng/ml as a capture antibody.)
WB (Western Blot) (CSE1L monoclonal antibody (M05), clone 3F8 Western Blot analysis of CSE1L expression in K-562.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CSE1L on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CSE1L on HeLa cell. [antibody concentration 10 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CSE1L on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to CSE1L on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml])
Application Data (Detection limit for recombinant GST tagged RUNX1 is approximately 1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RUNX1 on HeLa cell. [antibody concentration 40 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RUNX1 on HeLa cell. [antibody concentration 40 ug/ml])
WB (Western Blot) (RUNX1 monoclonal antibody (M06), clone 2C10 Western Blot analysis of RUNX1 expression in Hela S3 NE (Cat # L013V3).)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RUNX1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1.5 ug/ml])
Application Data (Immunoperoxidase of monoclonal antibody to RUNX1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1.5 ug/ml])
ELISA (Titration curve analysis of B7-H3 mAbs to detect recombinant B7-H3 in ELISA at decreasing concentrations.)
FCM (Flow Cytometry) (Flow cytometry analysis of B7-H3 in HEK293 cells using B7-H3 antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: B7-H3 over expressing HEK293 cells.)
IHC (Immunohistochemistry) (Immunohistochemistry of B7-H3 in human colon carcinoma tissue using B7-H3 Antibody and control mouse IgG (corner box) at 2 μg/ml.)
IF (Immunofluorescence) (Immunofluorescence of B7-H3 in human colon carcinoma tissue cells using B7-H3 Antibody at 10 μg/ml.Green: B7-H3 Antibody [4H3]Blue: DAPI staining)
IF (Immunofluorescence) (Immunofluorescence of B7-H3 in HEK293 cells using B7-H3 Antibody at 5 μg/ml.Green: B7-H3 Antibody [4H3]Blue: DAPI staining)
ICC (Immunocytochemistry) (Immunocytochemistry of B7-H3 in HEK293 cells using B7-H3 antibody and control mouse IgG antibody (left corner box) at 1 μg/ml.)
WB (Western Blot) (Western blot analysis of B7-H3 in HEK293 cells using B7-H3 antibody at (A) 0.25 (B) 0.5 and (C) 1 μg/ml.)
Application Data (Detection limit for recombinant GST tagged NR3C1 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NR3C1 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of NR3C1 expression in transfected 293T cell line by NR3C1 monoclonal antibody. Lane 1: NR3C1 transfected lysate (85.7kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (NR3C1 monoclonal antibody, Western Blot analysis of NR3C1 expression in HeLa NE.)
WB (Western Blot) (NR3C1 monoclonal antibody. Western Blot analysis of NR3C1 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)
WB (Western Blot) (ACTB monoclonal antibody (M03), clone 1E7. Western Blot analysis of ACTB expression in Raw 264.7.)
WB (Western Blot) (ACTB monoclonal antibody (M03), clone 1E7. Western Blot analysis of ACTB expression in PC-12.)
WB (Western Blot) (ACTB monoclonal antibody (M03), clone 1E7. Western Blot analysis of ACTB expression in NIH/3T3.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ACTB on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ACTB on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3 ug/ml])
WB (Western Blot) (ACTB monoclonal antibody (M03), clone 1E7. Western Blot analysis of ACTB expression in Jurkat (Cat # L017V1).)
ICC (Immunocytochemistry) (Confocal imaging of paraffin-embedded rat brain tissue using NeuN Rabbit mAb (AAA28482, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.)
ICC (Immunocytochemistry) (Confocal imaging of paraffin-embedded mouse brain tissue using NeuN Rabbit mAb (AAA28482, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.)
ICC (Immunocytochemistry) (Confocal imaging of paraffin-embedded human brain tissue using NeuN Rabbit mAb (AAA28482, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using NeuN Rabbit mAb (AAA28482) at a dilution of 1?2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using NeuN Rabbit mAb (AAA28482) at a dilution of 1?2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human brain tissue using NeuN Rabbit mAb (AAA28482) at a dilution of 1?2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)
WB (Western Blot) (Western blot analysis of various lysates using NeuN Rabbit mAb (AAA28482) at1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
Application Data (Published customer image: Representative images of the inflammatory changes in the facial nucleus during axonal regeneration, one week following facial nerve transaction. a, b: CD11b immunoreactivity for microglia is increased in the axotomized facial nucleus, and microglia enwrap the facial motor neurons, e.g. at arrows. The regenerating neurons were retrogradely labelled with fluorogold. c, d: CD6- positive T-cells accumulated in the injured motor nucleus (arrows). They had little cytoplasm but dense nuclei (c) and were sometimes clustered around neurons retrogradely labelled with fluorogold (d). The scale bar in (a) also applies to (b) and that in (c) also applies to (d).From: Shokouhi et al. BMC Neuroscience 2010 11:13.)
Application Data (Published customer image: CD11b immunoreactive (red) microglia in the motor cortex following cervical corticospinal tract injury. Representative images of motor cortex - a, b: one week (deconvolved images); c, d: two weeks following corticospinal tract injury. The corticospinal neurons were retrogradely labelled with fluorogold applied to the spinal cord at the time of injury. There is little sign of microglial activation. The inset in (c) shows a CD6-positive T-cell in the motor cortex, but no accumulation of such cells was detected.From: Shokouhi BN, Wong BZ, Siddiqui S, Lieberman AR, Campbell G, Tohyama K, Anderson PN. Microglial responses around intrinsic CNS neurons are correlated with axonal regeneration. BMC Neurosci. 2010 Feb 5;11:13.)
Application Data (Staining of rat peritoneal macrophages with Mouse anti Rat CD11b)
Application Data (Published customer image: Microglia in the red nucleus (a-d and inset) and motor cortex (e, f) 4 weeks after nerve graft implantation into the rubrospinal tract and cervical dorsal columns respectively. Neurons with regenerating axons were retrogradely labelled with fluorogold. Microglia established close contacts with regenerating rubrospinal neurons (arrows), which also expressed ATF3 (inset). No retrogradely labelled corticospinal neurons were detected (e and f) and there was no microglial activation in the motor cortex. (a-c) are non-deconvolved and (d-f plus inset) are deconvolved images. The scale bar in (a) also applies to (b).From: Shokouhi BN, Wong BZ, Siddiqui S, Lieberman AR, Campbell G, Tohyama K, Anderson PN. Microglial responses around intrinsic CNS neurons are correlated with axonal regeneration. BMC Neurosci. 2010 Feb 5;11:13.)
Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD11b antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a as a detection reagent. High power)
Application Data (Staining of rat peritoneal macrophages cells with Mouse anti Rat CD11b:Alexa Fluor 488)
Application Data (Published customer image: Rubrospinal injury produces little microglial activation and no accumulation of T-cells in the red nucleus. a, b: Representative images show there is little difference in CD11b immunoreactivity for microglia in the red nucleus (*) one week following axotomy. c, d: confocal images of beta thymosin immunoreactive microglia in the red nucleus 3 weeks after injury. e: a rare CD6-positive T lymphocyte in the red nucleus of an unoperated rat. f: one week after injury no T lymphocytes can be identified in the red nucleus around the axotomized rubrospinal neurons, which can be recognised by their expression of ATF3 (green). Neuronal cytoplasm has been visualised by high gain in the red signal; this does not represent CD6 signal.From: Shokouhi BN, Wong BZ, Siddiqui S, Lieberman AR, Campbell G, Tohyama K, Anderson PN. Microglial responses around intrinsic CNS neurons are correlated with axonal regeneration. BMC Neurosci. 2010 Feb 5;11:13.)
Application Data (Staining of rat peritoneal macrophages with Mouse anti Rat CD11b:RPE-Alexa Fluor 647)
Application Data (Immunofluoescence staining of rat lymph node cryosection with Mouse anti Rat CD11b antibody , red in A and Mouse anti Rat CD8 , green in B. C is the merged image with nuclei counter-stained in blue using DAPI. High power)
Application Data (Immunofluoescence staining of rat lymph node cryosection with Mouse anti Rat CD11b antibody , red in A and Mouse anti Rat CD8 , green in B. C is the merged image with nuclei counter-stained in blue using DAPI. Low power)
Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD11b:Biotin)
Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD11b:RPE)
Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD11b:Azide Free)
Application Data (Immunoperoxidase staining of human tonsil cryosection with Mouse anti Human CD11b antibody, clone ICRF44 followed by the Histar detection system . Low power)
Application Data (Immunoperoxidase staining of human spleen cryosection with Mouse anti Human CD11b antibody, clone ICRF44 followed by the Histar detection system . High power)
Application Data (Immunoperoxidase staining of human spleen cryosection with Mouse anti Human CD11b antibody, clone ICRF44 followed by the Histar detection system . Low power)
Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD11b:Alexa Fluor 647 (AAA11991A647))
Application Data (Immunofluorescence staining of human tonsil cryosection with Mouse anti Human CD11b antibody, clone ICRF44, red in A and Mousse anti Human CD21 , green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power)
Application Data (Immunofluorescence staining of human tonsil cryosection with Mouse anti Human CD11b antibody, clone ICRF44, red in A and Mousse anti Human CD21 , green in B. C is the merged image with nuclei counterstained blue using DAPI. medium power)
Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD11b:FITC)
WB (Western Blot) (BATF monoclonal antibody Western Blot analysis of BATF expression in Hela NE.)
WB (Western Blot) (BATF monoclonal antibody Western Blot analysis of BATF expression in PC-12.)
Application Data (Detection limit for recombinant GST tagged BATF is ~1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of BATF transfected lysate using BATF monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with BATF rabbit polyclonal antibody.)
WB (Western Blot) (Western Blot analysis of BATF expression in transfected 293T cell line by BATF monoclonal antibody Lane 1: BATF transfected lysate (14.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (BATF monoclonal antibody Western Blot analysis of BATF expression in NIH/3T3.)
WB (Western Blot) (Western Blot detection against Immunogen (36.23kD).)
WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)
Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between AKT1 and HSPB1. Mahlavu cells were stained with AKT1 rabbit purified polyclonal 1:1200 and HSPB1 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)
Application Data (Detection limit for recombinant GST tagged HSPB1 is ~0.1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of HSPB1 transfected lysate using HSPB1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with HSPB1 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HSPB1 on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of HSPB1 expression in transfected 293T cell line by HSPB1 monoclonal antibody. Lane 1: HSPB1 transfected lysate (22.8kD). Lane 2: Non-transfected lysate.)
Application Data (Detection limit for recombinant GST tagged MEOX2 is ~0.3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MEOX2 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to MEOX2 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 1ug/ml])
WB (Western Blot) (MEOX2 monoclonal antibody. Western Blot analysis of MEOX2 expression in Hela NE.)
WB (Western Blot) (MEOX2 monoclonal antibody. Western Blot analysis of MEOX2 expression in PC-12.)
WB (Western Blot) (MEOX2 monoclonal antibody Western Blot analysis of MEOX2 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (59.07kD).)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Stathmin 1 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining Stathmin 1 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Stathmin 1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Stathmin 1 in PC-12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Stathmin 1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Stathmin 1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse spinal cord tissue using anti-Stathmin 1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Stathmin 1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Stathmin 1 antibody. Counter stained with hematoxylin.)
ICC (Immunocytochemistry) (Confocal imaging of paraffin-embedded Rat brain tissue using NeuN Mouse mAb (AAA28598, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Mouse IgG (H+L) (AS008, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.)
ICC (Immunocytochemistry) (Confocal imaging of paraffin-embedded Mouse brain tissue using NeuN Mouse mAb (AAA28598, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Mouse IgG (H+L) (AS008, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.)
ICC (Immunocytochemistry) (Confocal imaging of paraffin-embedded Human brain tissue using NeuN Mouse mAb (AAA28598, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Mouse IgG (H+L) (AS008, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using NeuN Mouse mAb (AAA28598) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human brain tissue using NeuN Mouse mAb (AAA28598) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using NeuN Mouse mAb (AAA28598) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.)
WB (Western Blot) (Western blot analysis of various lysates using NeuN Mouse mAb (AAA28598) at 1:5000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)
ICC (Immunocytochemistry) (ICC staining Ubiquitin D in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Ubiquitin D in F9 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Ubiquitin D in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Ubiquitin D antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Ubiquitin D antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Ubiquitin D on Hela cells lysates using anti-Ubiquitin D antibody at 1/1, 000 dilution.)
ICC (Immunocytochemistry) (ICC staining EAAT3 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining EAAT3 in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining EAAT3 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-EAAT3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-EAAT3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-EAAT3 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-EAAT3 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of EAAT3 on mouse liver tissue lysate using anti-EAAT3 antibody at 1/500 dilution.)
IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using AIF Rabbit mAb (AAA28504) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using AIF Rabbit mAb (AAA28504) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using AIF Rabbit mAb (AAA28504) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using AIF Rabbit mAb (AAA28504) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human lung squamous carcinoma tissue using AIF Rabbit mAb (AAA28504) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human liver tissue using AIF Rabbit mAb (AAA28504) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using AIF Rabbit mAb (AAA28504) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using AIF Rabbit mAb (AAA28504) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
WB (Western Blot) (Western blot analysis of various lysates using AIF Rabbit mAb (AAA28504) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)
IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using Matrin 3 Rabbit mAb (AAA28449) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence) (Immunofluorescence analysis of NIH-3T3 cells using Matrin 3 Rabbit mAb (AAA28449) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence) (Immunofluorescence analysis of C6 cells using Matrin 3 Rabbit mAb (AAA28449) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse spinal cord using Matrin 3 Rabbit mAb (AAA28449) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human brain using Matrin 3 Rabbit mAb (AAA28449) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat ovary using Matrin 3 Rabbit mAb (AAA28449) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.)
WB (Western Blot) (Western blot analysis of lysates from Rat brain, using Matrin 3 Rabbit mAb (AAA28449) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)
WB (Western Blot) (Western blot analysis of various lysates using Matrin 3 Rabbit mAb (AAA28449) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
FCM (Flow Cytometry) (Flow cytometric analysis of K562 cells with TSG101 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining TSG101 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining TSG101 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining TSG101 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-TSG101 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-TSG101 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-TSG101 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TSG101 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of TSG101 on different lysates using anti-TSG101 antibody at 1/1, 000 dilution. Positive control: Lane 1: NIH/3T3 Lane 2: Hela Lane 3: K562 Lane 4: Jurkat Lane 5: A431)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Albumin antibody. Counter stained with hematoxylin.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Albumin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Albumin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Albumin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Albumin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Albumin antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Albumin on Hela cells lysates using anti-Albumin antibody at 1/1, 000 dilution.)
Application Data (Staining of human peripheral blood monocytes with MOUSE ANTI HUMAN CD274:RPE)
Application Data (Staining of human peripheral blood monocytes with C2549-22E4.)
Application Data (Staining of human peripheral blood monocytes with C2549-22E1.)
Application Data (Staining of human peripheral blood monocytes with C2549-22E1.)
Application Data (Jurkat cells were stained with C2549-22E (filled histogram) or isotype control (open histogram) followed by PE-conjugated anti-mouse antibody.)
Application Data (Staining of human peripheral blood monocytes with C2549-22E.)
Application Data (Detection limit for recombinant GST tagged NODAL is ~0.3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NODAL on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NODAL on formalin-fixed paraffin-embedded human endometrium cancer. [antibody concentration 3ug/ml])
WB (Western Blot) (NODAL monoclonal antibody Western Blot analysis of NODAL expression in PC-12.)
WB (Western Blot) (NODAL monoclonal antibody Western Blot analysis of NODAL expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (33.66kD).)
Application Data (Detection limit for recombinant GST tagged IFI16 is ~0.3ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to IFI16 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to IFI16 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of IFI16 expression in transfected 293T cell line by IFI16 monoclonal antibody. Lane 1: IFI16 transfected lysate (82kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (IFI16 monoclonal antibody Western Blot analysis of IFI16 expression in Hela NE.)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
IP (Immunoprecipitation) (Immunoprecipitation of USP33 transfected lysate using anti-USP33 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with USP33 rabbit polyclonal antibody.)
Application Data (Detection limit for recombinant GST tagged USP33 is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to USP33 on HeLa cell. [antibody concentration 20ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to USP33 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of USP33 expression in transfected 293T cell line by USP33 monoclonal antibody Lane 1: USP33 transfected lysate (103.3kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TIMP2 on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TIMP2 on HeLa cell. [antibody concentration 10 ug/ml])
Application Data (Detection limit for recombinant GST tagged TIMP2 is approximately 0.1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TIMP2 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TIMP2 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3 ug/ml])
WB (Western Blot) (TIMP2 monoclonal antibody (M03), clone 1C3 Western Blot analysis of TIMP2 expression in HeLa (Cat # L013V1).)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded Stomach. 1. Antibody was diluted at 1:200(4 degree overnight). 2. TRIS-EDTA of pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Stomach. 1. Antibody was diluted at 1:200(4 degree overnight). 2. TRIS-EDTA of pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Stomach. 1. Antibody was diluted at 1:200(4 degree overnight). 2. TRIS-EDTA of pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded Stomach. 1. Antibody was diluted at 1:200(4 degree overnight). 2. TRIS-EDTA of pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Stomach. 1. Antibody was diluted at 1:200(4 degree overnight). 2. TRIS-EDTA of pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Stomach. 1. Antibody was diluted at 1:200(4 degree overnight). 2. TRIS-EDTA of pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Stomach. 1. Antibody was diluted at 1:200(4 degree overnight). 2. TRIS-EDTA of pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Stomach. 1. Antibody was diluted at 1:200(4 degree overnight). 2. TRIS-EDTA of pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human Gastric adenocarcinoma Antibody was diluted at 1:200(4 degree overnight).)
Application Data (IMAB027 inhibited the tumor growth of OV90 on Balb/c nude mice. The result showed significant anti-tumor effects, with an tumor inhibition rate (TGI) of 50.7% at 5 mpk at D34.)
Application Data (Anti-CLDN6 Reference Antibody (IMAB027)by huCLDN6-HEK293 increased with the increase of antibody concentration, and the Internalization Rate (%) reached 60% at antibody concentration of 1 nM.)
Application Data (Anti-CLDN6 Reference Antibody (IMAB027)-induced ADCC activity was evaluated using Human CD16a (158V) (Luc) Jurkat Reporter Cell. The max induction fold was approximately 14.16)
FCM (Flow Cytometry) (Human CLDN6 CHO cells were stained with Anti-CLDN6 Reference Antibody (IMAB027) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC199=4.342 nM)
Application Data (Immobilized human CLDN6 VLP at 8 ug/mL can bind Anti-CLDN6 Reference Antibody (IMAB027), EC50=0.001599ug/mL)
Application Data (The purity of Anti-CLDN6 Reference Antibody (IMAB027)is more than 98.41% ,determined by SEC-HPLC.)
SDS-PAGE (Anti-CLDN6 Reference Antibody (IMAB027) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%)
ICC (Immunocytochemistry) (ICC staining Synaptophysin in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Synaptophysin in N2A cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-Synaptophysin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Synaptophysin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Synaptophysin antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Synaptophysin on different lysates using anti-Synaptophysin at 1/500 dilution. Positive control�� <br/>Lane 1: Human brain <br/>Lane 2: PC-12)
WB (Western Blot) (Western blot analysis of MRPL12 over-expressed 293 cell line, cotransfected with MRPL12 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with MRPL12 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged MRPL12 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MRPL12 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to MRPL12 on formalin-fixed paraffin-embedded human breast cancer tissue. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of MRPL12 expression in transfected 293T cell line by MRPL12 monoclonal antibody. Lane 1: MRPL12 transfected lysate (21.3kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (MRPL12 monoclonal antibody, Western Blot analysis of MRPL12 expression in COLO 320 HSR.)
WB (Western Blot) (Western blot analysis of RAD51C over-expressed 293 cell line, cotransfected with RAD51C Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with RAD51C monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged RAD51C is ~0.1ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RAD51C on HeLa cell. [antibody concentration 10ug/ml])
WB (Western Blot) (Western Blot analysis of RAD51C expression in transfected 293T cell line by RAD51C monoclonal antibody. Lane 1: RAD51C transfected lysate (42.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (RAD51C monoclonal antibody. Western Blot analysis of RAD51C expression in 293.)
WB (Western Blot) (RAD51C monoclonal antibody, Western Blot analysis of RAD51C expression in HeLa.)
WB (Western Blot) (Western blot analysis of PDK2 over-expressed 293 cell line, cotransfected with PDK2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PDK2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged PDK2 is ~0.3ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PDK2 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 0.8ug/ml])
WB (Western Blot) (Western Blot analysis of PDK2 expression in transfected 293T cell line by PDK2 monoclonal antibody. Lane 1: PDK2 transfected lysate (46.2kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (PDK2 monoclonal antibody Western Blot analysis of PDK2 expression in U-2 OS.)
WB (Western Blot) (Western Blot detection against Immunogen (35.31kD).)
Application Data (Detection limit for recombinant GST tagged SHMT1 is ~1ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SHMT1 on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 1ug/ml].)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to SHMT1 on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 1ug/ml].)
WB (Western Blot) (Western Blot analysis of SHMT1 expression in transfected 293T cell line by SHMT1 monoclonal antibody. Lane 1: SHMT1 transfected lysate (53.1kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (SHMT1 monoclonal antibody, Western Blot analysis of SHMT1 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (37.73kD).)
IF (Immunofluorescence) (Immunofluorescentstaining of PFA-fixedHeLa cells with Pan-Nuclear Antigen Monoclonal Antibody (AAA13844) followed by goat anti-mouse IgG-CF488 (green). Membranes labeled with phalloidin (red).)
SDS-PAGE (SDS-PAGE Analysis Purified Pan-Nuclear Antigen Monoclonal Antibody (AAA13844). Confirmation of Purity and Integrity of Antibody.)
IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded Rat Lung stained with Pan-Nuclear Ag Monoclonal Antibody (AAA13844).)
IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded Rat Colon stained with Pan-Nuclear Ag Monoclonal Antibody (AAA13844).)
IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Tonsil stained with Pan-Nuclear Ag Monoclonal Antibody (AAA13844).)
IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Tonsil stained with Pan-Nuclear Ag Monoclonal Antibody (AAA13844).)
ELISA (Titration curve analysis of LAG-3 mAbs to detect recombinant LAG-3 in ELISA at decreasing concentrations.)
FCM (Flow Cytometry) (Flow cytometry analysis of LAG-3 over expressing HEK293 cells using LAG-3 antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: LAG-3 over expressing HEK293 cells.)
IHC (Immunohistochemistry) (Immunohistochemistry of LAG-3 in human lymphoma tissue using LAG-3 Antibody and control mouse IgG (corner box) at 5 μg/ml.)
IF (Immunofluorescence) (Immunofluorescence of LAG-3 in over human spleen tissue using LAG-3 Antibody at 10 μg/ml.Green: LAG3 Antibody [6D1]Blue: DAPI staining)
IF (Immunofluorescence) (Immunofluorescence of LAG-3 in over expressing HEK293 cells using LAG-3 Antibody at 2 μg/ml.Green: LAG3 Antibody [6D1]Blue: DAPI staining)
ICC (Immunocytochemistry) (Immunocytochemistry of LAG-3 in over expressing HEK293 cells using LAG-3 antibody and control mouse IgG antibody (left corner box) at 1 μg/ml.)
Application Data (Proximity Ligation Analysis of protein-protein interactions between MSH2 and MLH1 HeLa cells were stained with anti-MSH2 rabbit purified polyclonal 1:1200 and anti-MLH1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
Application Data (Detection limit for recombinant GST tagged MLH1 is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MLH1 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to MLH1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 1ug/ml])
WB (Western Blot) (MLH1 monoclonal antibody Western Blot analysis of MLH1 expression in HeLa NE.)
WB (Western Blot) (Western Blot detection against Immunogen (108.9kD).)
ICC (Immunocytochemistry) (ICC staining EGFR in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining EGFR in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining EGFR in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-EGFR antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-EGFR antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-EGFR antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-EGFR antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-EGFR antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of EGFR on different cell lysates using anti-EGFR at 1/500 dilution. Positive control�� Line 1: Hela Line 2: A431 Line 3: HUVEC)
IHC (Immunohistchemistry) (Human pancreas tissue was stained with Anti-MUC1 (ABT100) Antibody)
IHC (Immunohistochemistry) (Human mesothelioma tissue was stained with Anti-MUC1 (ABT100) Antibody)
IHC (Immunohistochemistry) (Human lung adenocarcinoma tissue was stained with Anti-MUC1 (ABT100) Antibody)
IHC (Immmunohistochemistry) (Human kidney tissue was stained with Anti-MUC1 (ABT100) Antibody)
IHC (Immunohistochemistry) (Human gastric adenocarcinoma tissue was stained with Anti-MUC1 (ABT100) Antibody)
SDS-PAGE (Whole cell lysates of NIH3T3 were separated by 8% SDS-PAGE, and the membrane was blotted with anti-MUC1 antibody. The HRP-conjugated anti-Mouse IgG antibody was used to detect the antibody.)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with 14-3-3 alpha+beta antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining 14-3-3 alpha+beta in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining 14-3-3 alpha+beta in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining 14-3-3 alpha+beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-14-3-3 alpha+beta antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse skin tissue using anti-14-3-3 alpha+beta antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-14-3-3 alpha+beta antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-14-3-3 alpha+beta antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of 14-3-3 alpha+beta on different lysates using anti-14-3-3 alpha/beta antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: 293T Lane 3: HepG2)
ICC (Immunocytochemistry) (ICC staining Glutathione Peroxidase 1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Glutathione Peroxidase 1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Glutathione Peroxidase 1 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Glutathione Peroxidase 1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Glutathione Peroxidase 1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Glutathione Peroxidase 1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Glutathione Peroxidase 1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Glutathione Peroxidase 1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Glutathione Peroxidase 1 on THP-1 cells lysates using anti-Glutathione Peroxidase 1 antibody at 1/1, 000 dilution.)
ICC (Immunocytochemistry) (ICC staining PKC delta in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining PKC delta in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining PKC delta in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-PKC delta antibody. Counter stained with hematoxylin.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-PKC delta antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-PKC delta antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PKC delta antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-PKC delta antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PKC delta antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of PKC delta on different lysates using anti-PKC delta antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3)
WB (Western Blot) (LETM1 monoclonal antibody Western Blot analysis of LETM1 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (37.62kD).)
Application Data (Detection limit for recombinant GST tagged LETM1 is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of LETM1 transfected lysate using LETM1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LETM1 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to LETM1 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to LETM1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of LETM1 expression in transfected 293T cell line by LETM1 monoclonal antibody. Lane 1: LETM1 transfected lysate (83.4kD). Lane 2: Non-transfected lysate.)
LETM1 and EF-hand Domain Containing Protein 1, Mitochondrial (LETM1, Leucine Zipper-EF-hand-containing Transmembrane Protein 1) (FITC)
Reactivity
Human
Applications
Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot
Purity
Purified by Protein A Affinity Chromatography.
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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