Monoclonal Antibodies

Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with MEKK3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining MEKK3 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining MEKK3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining MEKK3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat uterus tissue using anti-MEKK3 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse uterus tissue using anti-MEKK3 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of MEKK3 on different lysates using anti-MEKK3 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: 293T Lane 3: A431)

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MEKK3, Monoclonal Antibody (Cat# AAA30219)

Full Name

MEKK3 Antibody

Gene Names

MAP3K3; MEKK3; MAPKKK3

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with gamma Catenin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)

ICC (Immunocytochemistry) (ICC staining gamma Catenin in A431 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining gamma Catenin in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining gamma Catenin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-gamma Catenin antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of gamma Catenin on Hela cells lysates using anti-gamma Catenin antibody at 1/1, 000 dilution.)

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gamma Catenin, Monoclonal Antibody (Cat# AAA30273)

Full Name

gamma Catenin Antibody

Gene Names

JUP; DP3; PDGB; PKGB; CTNNG; DPIII; ARVD12

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of SH-SY5Y cells with DDX5 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)

ICC (Immunocytochemistry) (ICC staining DDX5 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining DDX5 in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining DDX5 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-DDX5 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-DDX5 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-DDX5 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of DDX5 on different cell lysate using anti-DDX5 antibody at 1/1, 000 dilution. Positive control�� Lane1: PC-12 Lane2: MEF Lane3: SH-SY5Y Lane4: NIH-3T3 Lane5: Mouse brain)

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DDX5, Monoclonal Antibody (Cat# AAA30370)

Full Name

DDX5 Antibody

Gene Names

DDX5; p68; HLR1; G17P1; HUMP68

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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Histone H3, Monoclonal Antibody (Cat# AAA30816)

Full Name

Histone H3 (Tri Methyl Lys79) Monoclonal Antibody (3G3)

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation

Purity

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Pricing

$325/0.04 mL $445/0.1 mL $685/0.2 mL $1,310/2x0.2 mL $1,905/3x0.2 mL $2,460/4x0.2 mL $2,760/5x0.2 mL

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Application Data (Detection limit for recombinant GST tagged FGF8 is ~0.3ng/ml as a capture antibody.)

WB (Western Blot) (FGF8 monoclonal antibody Western Blot analysis of FGF8 expression in NIH/3T3.)

WB (Western Blot) (FGF8 monoclonal antibody Western Blot analysis of FGF8 expression in Jurkat.)

WB (Western Blot) (FGF8 monoclonal antibody. Western Blot analysis of FGF8 expression in Raw 264.7.)

WB (Western Blot) (FGF8 monoclonal antibody, Western Blot analysis of FGF8 expression in HepG2.)

WB (Western Blot) (FGF8 monoclonal antibody. Western Blot analysis of FGF8 expression in PC-12.)

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Fibroblast Growth Factor 8, Monoclonal Antibody (Cat# AAA25393)

Full Name

Fibroblast Growth Factor 8 (FGF8, FGF-8, Androgen-induced Growth Factor, AIGF, Heparin-binding Growth Factor 8, HBGF-8) (HRP)

Gene Names

FGF8; HH6; AIGF; KAL6; FGF-8; HBGF-8

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

Application Data (COVI19-IgG/IgM products from three different companies (K, LZ and INT company) were tested. All of them showed obvious band when using over 1 ul anti-2019-nCoV N IgM (NM01) (AAA27967). For the INT product, it showed obvious IgM band even at 0.5 ul NM01 (AAA27967). No non-specific IgG band was shown in this test.)

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COVID 19 Nucleocapsid (NP) Humanized Coronavirus, Monoclonal Antibody (Cat# AAA27967)

Full Name

Anti-SARS-CoV-2 Nucleocapsid IgM/COVID-19, Humanized antibody (Positive Control)

Pricing

$905/0.1 mg $1,735/2x0.1 mg $2,520/3x0.1 mg $3,265/4x0.1 mg $3,620/5x0.1 mg

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IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded esophageal tissues using DSG3 mouse mAb with DAB staining.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded esophageal cancer tissues using DSG3 mouse mAb with DAB staining.)

FCM (Flow Cytometry) (Flow cytometric analysis of A431 cells using DSG3 mouse mAb (green) and negative control (red).)

WB (Western Blot) (Western blot analysis using DSG3 mouse mAb against A431 cell lysate.)

WB (Western Blot) (Western blot analysis using DSG3 mAb against HEK293 (1) and DSG3 (AA)

WB (Western Blot) (Western blot analysis using DSG3 mAb against human DSG3 (AA)

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DSG3, Monoclonal Antibody (Cat# AAA14149)

Full Name

Anti-DSG3 Mouse mAb

Gene Names

DSG3; PVA; CDHF6

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Pricing

$565/0.1 mL $1,070/2x0.1 mL $1,545/3x0.1 mL $1,900/5x0.1 mL $1,995/4x0.1 mL

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WB (Western Blot) (KIF2C monoclonal antibody Western Blot analysis of KIF2C expression in HeLa NE.)

WB (Western Blot) (Western Blot analysis of KIF2C expression in transfected 293T cell line by KIF2C monoclonal antibody Lane 1: KIF2C transfected lysate (81.3kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western blot analysis of KIF2C over-expressed 293 cell line, cotransfected with KIF2C Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with KIF2C monoclonal antibody (M01). GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged KIF2C is ~0.1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of KIF2C transfected lysate using KIF2C monoclonal antibody and Protein A Magnetic Bead and immunoblotted with KIF2C rabbit polyclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to KIF2C on HeLa cell. [antibody concentration 10ug/ml].)

Application Data (Immunoperoxidase of monoclonal antibody to KIF2C on formalin-fixed paraffin-embedded human malignant lymphoma, diffuse large B. [antibody concentration 3ug/ml].)

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Kinesin 2C, Monoclonal Antibody (Cat# AAA25731)

Full Name

Kinesin 2C (KIF2C, Kinesein Family Member 2C, Kinesin-Like Protein 6, KNSL6, Kinesin-like Protein KIF2C, Mitotic Centromere-Associated Kinesin, MCAK, 4930402F02Rik, ESTM5, MGC11883, OTTHUMP00000010066, X83316) (PE)

Gene Names

KIF2C; MCAK; CT139; KNSL6

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MCM3 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MCM3 on HeLa cell. [antibody concentration 10 ug/ml])

Application Data (Detection limit for recombinant GST tagged MCM3 is approximately 0.1ng/ml as a capture antibody.)

WB (Western Blot) (MCM3 monoclonal antibody (M04), clone 2H3. Western Blot analysis of MCM3 expression in Y-79 (Cat # L042V1).)

WB (Western Blot) (MCM3 monoclonal antibody (M04), clone 2H3. Western Blot analysis of MCM3 expression in human ovarian cancer.)

WB (Western Blot) (MCM3 monoclonal antibody (M04), clone 2H3. Western Blot analysis of MCM3 expression in Hela S3 NE (Cat # L013V3).)

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MCM3, Monoclonal Antibody (Cat# AAA26246)

Full Name

MCM3 (Minichromosome Maintenance Complex Component 3, HCC5, MGC1157, P1-MCM3, P1.h, RLFB) (Biotin)

Gene Names

MCM3; HCC5; P1.h; RLFB; P1-MCM3

Applications

Immunofluorescence, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Staining of mouse spleen cells with HAMSTER ANTI MOUSE CD29: Alexa Fluor® 647)

Application Data (Staining of mouse spleen cells with hamster anti mouse CD29: Alexa Fluor® 488)

Application Data (Staining of mouse peripheral blood lymphocytes with HAMSTER ANTI MOUSE CD29:FITC)

Application Data (Staining of mouse peripheral blood lymphocytes with HAMSTER ANTI MOUSE CD29: LOW ENDOTOXIN)

Application Data (Staining of mouse peripheral blood lymphocytes with HAMSTER ANTI MOUSE CD29)

Application Data (Staining of mouse peripheral blood lymphocytes with HAMSTER ANTI MOUSE CD29)

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Integrin, beta1, Monoclonal Antibody (Cat# AAA26801)

Full Name

Integrin, beta1 (CD29, Very Late Antigen, VLAb1, Platelet gplla, ITGB1, Fibrinogen Receptor beta Subunit, FNRB, Integrin VLA4 Subunit beta, MDF2, MSK12, Very Late Activation Protein beta Polypeptide, VLAB, VLAbeta) (MaxLight 490)

Reactivity

Mouse, Rat

Applications

Flow Cytometry, Immunoprecipitation

Purity

Purified by Protein G Affinity Chromatography

Pricing

$1,010/0.1 mL $1,950/2x0.1 mL $2,835/3x0.1 mL $3,675/4x0.1 mL $4,260/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Rat-kidney tissue. 1.MAP2 Monoclonal Antibody(7D4) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Mouse-brain tissue. 1.MAP2 Monoclonal Antibody(7D4) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1.MAP2 Monoclonal Antibody(7D4) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human pancreas. 1. Antibody was diluted at 1:400(4 degree overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human pancreas. 1. Antibody was diluted at 1:400(4 degree overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human pancreas. 1. Antibody was diluted at 1:400(4 degree overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human pancreas. 1. Antibody was diluted at 1:400(4 degree overnight). 2. High-pressure and temperature EDTA. pH8.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)

IHC (Immunohistochemistry) (IHC staining of Human brain tissue paraffin-embedded. diluted at 1:200.)

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MAP2, Monoclonal Antibody (Cat# AAA30810)

Full Name

MAP2 Monoclonal Antibody (7D4)

Gene Names

MAP2; MAP2A; MAP2B; MAP2C

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Pricing

$300/0.04 mL $390/0.1 mL $555/0.2 mL $1,045/2x0.2 mL $1,510/3x0.2 mL $1,955/4x0.2 mL $2,150/5x0.2 mL

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ICC (Immunocytochemistry) (ICC staining Collagen VI in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Collagen VI in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Collagen VI in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Collagen VI in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Collagen VI antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Collagen VI antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Collagen VI antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Collagen VI on different lysates using anti-Collagen VI antibody at 1/1, 000 dilution. Positive control: Lane 1: Mouse heart Lane 2: Human heart Lane 3: Human kidney)

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Collagen VI, Monoclonal Antibody (Cat# AAA30217)

Full Name

Collagen VI Antibody

Gene Names

COL6A1; OPLL

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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Application Data (Staining of Rat peripheral blood lymphocytes with Mouse anti Rat CD49d:RPE (MCA2872PE))

Application Data (Staining of Rat peripheral blood lymphocytes with Mouse anti Rat CD49d : Alexa Fluor® 488 (MCA2872A488))

Application Data (Staining of Rat peripheral blood lymphocytes with Mouse anti Rat CD49d : Alexa Fluor® 647 (MCA2872A647))

Application Data (Staining of Rat peripheral blood lymphocytes with Mouse anti Rat CD49d (MCA2872)

Application Data (Staining of Rat peripheral blood lymphocytes with Mouse anti Rat CD49d:FITC (MCA2872F)

Application Data (Staining of Rat peripheral blood lymphocytes with Mouse anti Rat CD49d (MCA2872GA))

Application Data (Staining of Rat peripheral blood lymphocytes with Mouse anti Rat CD49d (MCA2872T))

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CD49d, Monoclonal Antibody (Cat# AAA26771)

Full Name

CD49d (Antigen CD49d, CD49d Antigen, CDw49d, Alpha 4 Subunit of VLA-4 Receptor, Integrin alpha IV, Integrin alpha 4, IA4, ITGA4, LPAM23, MGC90518, Very Late Activation Protein 4 Receptor Alpha 4 Subunit, VLA4, VLA-4) (PE)

Reactivity

Rat

Applications

Flow Cytometry, Immunoprecipitation

Purity

Purified by Protein G Affinity Chromatography

Pricing

$1,010/0.1 mL $1,950/2x0.1 mL $2,835/3x0.1 mL $3,675/4x0.1 mL $4,260/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometry analysis of LIGHT overexpressing HEK293 cells using LIGHT antibody and control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: LIGHT overexpressing HEK293 cells.)

IHC (Immunohistochemistry) (Immunohistochemistry of LIGHT in human lymphoma tissue with LIGHT antibody at 5 μg/mL.)

IF (Immunofluorescence) (Immunofluorescence of LIGHT in human lymphoma tissue with LIGHT antibody at 20 μg/mL.Green: LIGHT Antibody [8D4]Blue: DAPI staining)

IF (Immunofluorescence) (Immunofluorescence of LIGHT in transfected HEK293 cells with LIGHT antibody at 2 μg/mL.Green: LIGHT Antibody [8D4]Blue: DAPI staining)

ICC (Immunocytochemistry) (Immunocytochemistry of LIGHT in transfected HEK293 cells with LIGHT antibody at 1 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 μg/mL.)

WB (Western Blot) (Western blot analysis of LIGHT in overexpressing HEK293 cells with LIGHT antibody at 0.5 and 1 μg/ml)

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LIGHT, Monoclonal Antibody (Cat# AAA11011)

Full Name

LIGHT Antibody [8D4]

Gene Names

TNFSF14; LTg; CD258; HVEML; LIGHT

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Protein A purified

Pricing

$570/0.1 mg $1,080/2x0.1 mg $1,560/3x0.1 mg $2,015/4x0.1 mg $2,295/5x0.1 mg

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Application Data (Proximity Ligation Analysis of protein-protein interactions between TP53 and PML HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-PML mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)

Application Data (Detection limit for recombinant GST tagged PML is ~0.3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PML on HeLa cell. [antibody concentration 10ug/ml])

WB (Western Blot) (PML monoclonal antibody Western Blot analysis of PML expression in Hela NE.)

WB (Western Blot) (PML monoclonal antibody Western Blot analysis of PML expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (36.63kD).)

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PML, Monoclonal Antibody (Cat# AAA24619)

Full Name

PML (Probable Transcription Factor PML, RING Finger Protein 71, Tripartite Motif-containing Protein 19, PML, MYL, RNF71, TRIM19) APC

Gene Names

PML; MYL; RNF71; PP8675; TRIM19

Reactivity

Human, Rat

Applications

Immunofluorescence, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (HSPA1B monoclonal antibody Western Blot analysis of HSPA1B expression in Raw 264.7.)

WB (Western Blot) (HSPA1B monoclonal antibody Western Blot analysis of HSPA1B expression in NIH/3T3)

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between TP53 and HSPA1B. HeLa cells were stained with TP53 rabbit purified polyclonal 1:1200 and HSPA1B mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Application Data (Detection limit for recombinant GST tagged HSPA1B is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HSPA1B on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HSPA1B on formalin-fixed paraffin-embedded human testis. [antibody concentration 6ug/ml])

WB (Western Blot) (Western Blot detection against Immunogen (34.65kD).)

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HSPA1A, Monoclonal Antibody (Cat# AAA25423)

Full Name

HSPA1A (Heat Shock 70kD Protein 1A/1B, Heat Shock 70kD Protein 1/2, HSP70.1/HSP70.2, HSP70-1/HSP70-2, HSPA1, HSPA1B) (HRP)

Gene Names

HSPA1A; HSP72; HSPA1; HSP70I; HSP70-1; HSP70.1; HSP70-1A; HEL-S-103

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (BACE1 monoclonal antibody (M02), clone 2C1. Western Blot analysis of BACE1 expression in rat testis.)

WB (Western Blot) (Western Blot analysis of BACE1 expression in transfected 293T cell line by BACE1 monoclonal antibody (M01), clone 2C1.Lane 1: BACE1 transfected lysate (52.9 KDa).Lane 2: Non-transfected lysate.)

Application Data (Proximity Ligation Analysis of protein-protein interactions between MMP2 and BACE1. HeLa cells were stained with anti-MMP2 rabbit purified polyclonal 1:1200 and anti-BACE1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)

Application Data (Detection limit for recombinant GST tagged BACE1 is approximately 0.03ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to BACE1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to BACE1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml])

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BACE1, Monoclonal Antibody (Cat# AAA26285)

Full Name

BACE1 (beta-site APP-cleaving Enzyme 1, ASP2, BACE, FLJ90568, HSPC104, KIAA1149) (Biotin)

Gene Names

BACE1; ASP2; BACE; HSPC104

Applications

Immunohistochemistry, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of THP-1 cells with TLR2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)

ICC (Immunocytochemistry) (ICC staining TLR2 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining TLR2 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining TLR2 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-TLR2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-TLR2 antibody. Counter stained with hematoxylin.)

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TLR2, Monoclonal Antibody (Cat# AAA30387)

Full Name

TLR2 Antibody

Gene Names

TLR2; TIL4; CD282

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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Application Data

Application Data

SDS-PAGE (Whole cell lysates were separated by 8% SDS-PAGE, and the membrane was blotted with anti-P40(ABT-P40) antibody. The HRP-conjugated)

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p40, Monoclonal Antibody (Cat# AAA30913)

Full Name

p40 (ABT-P40) Antibody

Gene Names

TP63; AIS; KET; LMS; NBP; RHS; p40; p51; p63; EEC3; OFC8; p73H; p73L; SHFM4; TP53L; TP73L; p53CP; TP53CP; B(p51A); B(p51B)

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot, Immunofluorescence

Purity

Protein G

Pricing

$240/0.02 mL $315/0.05 mL $375/0.1 mL $600/0.2 mL $1,140/2x0.2 mL $1,650/3x0.2 mL $2,135/4x0.2 mL $2,350/5x0.2 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of MCF-7 cells with MUC1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining MUC1 in B16-F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining MUC1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-MUC1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-MUC1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-MUC1 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of MUC1 on Hela cells lysates using anti-MUC1 antibody at 1/1, 000 dilution.)

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MUC1, Monoclonal Antibody (Cat# AAA30155)

Full Name

MUC1 Antibody

Gene Names

MUC1; EMA; PEM; PUM; KL-6; MAM6; PEMT; CD227; H23AG; MUC-1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC

Reactivity

Human

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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ICC (Immunocytochemistry) (ICC staining Phospho-STAT3 (S727) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Phospho-STAT3 (S727) in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Phospho-STAT3 (S727) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Phospho-STAT3 (S727) antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Phospho-STAT3 (S727) antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Phospho-STAT3 (S727) antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat liver tissue using anti-Phospho-STAT3 (S727) antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Phospho-STAT3 (S727) on different lysates using anti-Phospho-STAT3 (S727) antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3)

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STAT3, Monoclonal Antibody (Cat# AAA29771)

Full Name

STAT3 (Phospho-S727) Antibody

Gene Names

STAT3; APRF; HIES

Reactivity

Human, Rat, Mouse

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation

Purity

ProA affinity purified

Pricing

$390/0.05 mL $510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,015/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using Ferritin Light Chain Rabbit mAb (AAA28453) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using Ferritin Light Chain Rabbit mAb (AAA28453) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Ferritin Light Chain Rabbit mAb (AAA28453) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human thyroid tissue using Ferritin Light Chain Rabbit mAb (AAA28453) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

WB (Western Blot) (Western blot analysis of lysates from Rat liver using Ferritin Light Chain Rabbit mAb (AAA28453) at 1:2000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 5s.)

WB (Western Blot) (Western blot analysis of various lysates using Ferritin Light Chain Rabbit mAb (AAA28453) at 1:2000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 5s.)

WB (Western Blot) (Western blot analysis of various lysates using Ferritin Light Chain Rabbit mAb (AAA28453) at 1:2000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.)

WB (Western Blot) (Western blot analysis of lysates from wild type (WT) and Ferritin Light Chain knockdown (KD) 293T cells using Ferritin Light Chain Rabbit mAb (AAA28453) at 1:2000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 60s.)

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Ferritin Light Chain, Monoclonal Antibody (Cat# AAA28453)

Full Name

Ferritin Light Chain Rabbit mAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Purity

Affinity purification

Pricing

$300/0.02 mL $500/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,780/4x0.1 mL $1,960/5x0.1 mL

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 600 ug extracts of 293T cells using 3 ug [KD Validated] CDX2 Rabbit mAb (AAA28469). Western blot was performed from the immunoprecipitate using CDX2 antibody (AAA28469) at a dilution of 1:500.)

ICC (Immunocytochemistry) (Confocal imaging of Human colon using [KD Validated] CDX2 Rabbit mAb (AAA28469,dilution 1:100)(Red). DAPI was used for nuclear staining (blue). Objective: 40x.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using [KD Validated] CDX2 Rabbit mAb (AAA28469) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human appendix using [KD Validated] CDX2 Rabbit mAb (AAA28469) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.)

WB (Western Blot) (Western blot analysis of lysates from wild type(WT) and CDX2 knockdown (KD) 293T cells, using [KD Validated] CDX2 Rabbit mAb (AAA28469) at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)

WB (Western Blot) (Western blot analysis of various lysates using [KD Validated] CDX2 Rabbit mAb (AAA28469) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 3min.)

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CDX2, Monoclonal Antibody (Cat# AAA28469)

Full Name

[KD Validated] CDX2 Rabbit mAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation

Purity

Affinity purification

Pricing

$300/0.02 mL $500/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,780/4x0.1 mL $1,960/5x0.1 mL

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SDS-PAGE (Purified ER-beta Mouse Monoclonal Antibody (ESR2/686). Confirmation of Integrity and Purity of Antibody.)

FCM (Flow Cytometry) (Flow Cytometry for human ER-beta on MCF-7 Cells. Black: Cells alone; Green: Isotype Control; Red: PE-labeled ER-beta1 Monoclonal Antibody (ESR2/686).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with ER-beta1 Monoclonal Antibody (ESR2/686).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with ER-beta1 Monoclonal Antibody (ESR2/686).)

FCM (Flow Cytometry) (Flow Cytometry of human ER beta on BT474 Cells. Black: Cells alone; Grey: Isotype Control; Green: AF488-labeled ER beta1 Monoclonal Antibody (ESR2/686).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Gastric Carcinoma stained with ER-beta1 Monoclonal Antibody (ESR2/686).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Bladder Carcinoma stained with ER-beta1 Monoclonal Antibody (ESR2/686).)

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ER-beta1 (Estrogen Receptor beta-1), Monoclonal Antibody (Cat# AAA13846)

Full Name

ER-beta1 (Estrogen Receptor beta-1) Mouse Monoclonal Antibody

Gene Names

ESR2; Erb; ESRB; ESTRB; NR3A2; ER-BETA; ESR-BETA

Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry

Pricing

$295/0.02 mg (With BSA & Azide at 0.2mg/mL) $475/0.1 mg (With BSA & Azide at 0.2mg/mL) $475/0.1 mg (Without BSA & Azide at 1mg/mL) $905/2x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,310/3x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,690/4x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,900/5x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,900/5x0.1 mg (Without BSA & Azide at 1mg/mL)

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WB (Western Blot) (Detection of human AXL by western blot. Samples: Whole cell lysate (10 ug) from OVCAR-8, HEK293T, HeLa, Jurkat, and 786-O cells prepared using NETN lysis buffer. Antibody: Rabbit anti-AXL recombinant monoclonal antibody (AAA23873 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes.)

WB (Western Blot) (Detection of mouse AXL by western blot. Samples: Whole cell lysate (25 ug) from AML12, C2C12, CT26, TCMK-1, and 4T1 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-AXL recombinant monoclonal antibody (AAA23873 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

IP (Immunoprecipitation) (Detection of human AXL by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from OVCAR-8 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-AXL recombinant monoclonal antibody (AAA23873 lot 1) used for IP at 6 ul/mg lysate. AXL was also immunoprecipitated by an antibody against a different epitope of AXL and a polyclonal antibody against the same epitope of AXL . For blotting immunoprecipitated AXL, AAA23873 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 10 seconds.)

ICC (Immunocytochemistry) (Detection of mouse AXL by immunocytochemistry. Sample: FFPE section of AML12 cells. Antibody: Rabbit anti-AXL recombinant monoclonal antibody (AAA23873-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

ICC (Immunocytochemistry) (Detection of human AXL by immunocytochemistry. Sample: FFPE section of HDLM-2 cells. Antibody: Rabbit anti-AXL recombinant monoclonal antibody (AAA23873-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

ICC (Immunocytochemistry) (Detection of human AXL by immunocytochemistry. Sample: FFPE section of DU145 cells. Antibody: Rabbit anti-AXL recombinant monoclonal antibody (AAA23873-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

FCM (Flow Cytometry) (Detection of human AXL (shaded) in OVCAR8 cells by flow cytometry. Antibody: Rabbit anti-AXL recombinant monoclonal antibody (AAA23873) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

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AXL, Monoclonal Recombinant Antibody (Cat# AAA23873)

Full Name

Rabbit anti-AXL Recombinant Monoclonal Antibody [BLR222K]

Gene Names

AXL; ARK; UFO; JTK11; Tyro7

Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation, Immunocytochemistry, Flow Cytometry

Purity

Purified

Pricing

$295/0.01 mL $680/0.1 mL $980/5x0.01 mL $1,300/2x0.1 mL $1,890/3x0.1 mL $2,440/4x0.1 mL $2,755/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ROD1 on HeLa cell. [antibody concentration 20ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ROD1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml])

WB (Western Blot) (ROD1 monoclonal antibody. Western Blot analysis of ROD1 expression in NIH/3T3)

WB (Western Blot) (ROD1 monoclonal antibody Western Blot analysis of ROD1 expression in Hela NE.)

WB (Western Blot) (ROD1 monoclonal antibody Western Blot analysis of ROD1 expression in PC-12)

WB (Western Blot) (Western Blot detection against Immunogen (36.63kD).)

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ROD1, Monoclonal Antibody (Cat# AAA24932)

Full Name

ROD1 (PTBP3, Polypyrimidine Tract-binding Protein 3, Regulator of Differentiation 1) (Biotin)

Gene Names

PTBP3; ROD1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MAPK9 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MAPK9 on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (MAPK9 monoclonal antibody (M05), clone 1D6. Western Blot analysis of MAPK9 expression in Raw 264.7.)

WB (Western Blot) (MAPK9 monoclonal antibody (M05), clone 1D6. Western Blot analysis of MAPK9 expression in PC-12.)

WB (Western Blot) (MAPK9 monoclonal antibody (M05), clone 1D6. Western Blot analysis of MAPK9 expression in human liver.)

WB (Western Blot) (MAPK9 monoclonal antibody (M05), clone 1D6. Western Blot analysis of MAPK9 expression in HepG2 (Cat # L019V1).)

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MAPK9, Monoclonal Antibody (Cat# AAA26139)

Full Name

MAPK9 (Mitogen-Activated Protein Kinase 9, JNK-55, JNK2, JNK2A, JNK2ALPHA, JNK2B, JNK2BETA, PRKM9, SAPK, p54a, p54aSAPK) (APC)

Gene Names

MAPK9; JNK2; SAPK; p54a; JNK2A; JNK2B; PRKM9; JNK-55; SAPK1a; JNK2BETA; p54aSAPK; JNK2ALPHA

Applications

Immunofluorescence, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged HDAC1 is approximately 1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HDAC1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 0.7 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HDAC1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 0.7 ug/ml])

WB (Western Blot) (HDAC1 monoclonal antibody (M11), clone 5A11 Western Blot analysis of HDAC1 expression in Hela S3 NE.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HDAC1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HDAC1 on HeLa cell. [antibody concentration 10 ug/ml])

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HDAC1, Monoclonal Antibody (Cat# AAA25921)

Full Name

HDAC1 (Histone Deacetylase 1, DKFZp686H12203, GON-10, HD1, RPD3, RPD3L1) (AP)

Gene Names

HDAC1; HD1; RPD3; KDAC1; GON-10; RPD3L1

Applications

Immunohistochemistry, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of GAPDH expression in transfected 293T cell line by GAPDH monoclonal antibody (M01), clone 3C2.Lane 1: GAPDH transfected lysate (36.1 KDa).Lane 2: Non-transfected lysate.)

WB (Western Blot) (GAPDH monoclonal antibody (M01), clone 3C2. Western Blot analysis of GAPDH expression in Raw 264.7.)

WB (Western Blot) (GAPDH monoclonal antibody (M01), clone 3C2. Western Blot analysis of GAPDH expression in PC-12.)

WB (Western Blot) (GAPDH monoclonal antibody (M01), clone 3C2. Western Blot analysis of GAPDH expression in NIH/3T3.)

WB (Western Blot) (GAPDH monoclonal antibody (M01), clone 3C2. Western Blot analysis of GAPDH expression in HeLa (Cat # L013V1).)

WB (Western Blot) (GAPDH monoclonal antibody (M01), clone 3C2 Western Blot analysis of GAPDH expression in A-431 (Cat # L015V1).)

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GAPDH, Monoclonal Antibody (Cat# AAA26252)

Full Name

GAPDH (Glyceraldehyde-3-Phosphate Dehydrogenase, G3PD, GAPD, MGC88685) (Biotin)

Gene Names

GAPDH; G3PD; GAPD; HEL-S-162eP

Applications

Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data

Application Data

Application Data

Application Data

Application Data

Application Data

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CD2, Monoclonal Antibody (Cat# AAA26753)

Full Name

CD2 (CD2 Antigen, LFA3 Receptor, Lymphocyte Function Antigen 2, LFA2, p50, Rosette Receptor, Sheep Erythrocyte Receptor, Sheep Red Blood Cell Receptor, SRBC, T Cell Surface Antigen CD2, T Cell Surface Antigen T11/Leu-5, T11) (HRP)

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunoprecipitation

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,010/0.1 mL $1,950/2x0.1 mL $2,835/3x0.1 mL $3,675/4x0.1 mL $4,260/5x0.1 mL

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ICC (Immunocytochemistry) (ICC staining CD21 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining CD21 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human gastric cancer tissue using anti-CD21 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-CD21 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-CD21 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of CD21 on different lysates using anti-CD21 antibody at 1/1, 000 dilution. Positive control: Lane 1: Daudi Lane 2: Raji)

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CD21, Monoclonal Antibody (Cat# AAA30132)

Full Name

CD21 Antibody

Gene Names

CR2; CR; C3DR; CD21; CVID7; SLEB9

Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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Application Data

Application Data

Application Data

Application Data

Application Data

SDS-PAGE (Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Actin, muscle specific (ABT-MSA) antibody. The HRP-conjugated Goat anti-M)

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Actin, Muscle Specific, Monoclonal Antibody (Cat# AAA30864)

Full Name

Actin, Muscle Specific (ABT-MSA) Antibody

Gene Names

ACTC1; ACTC; ASD5; CMD1R; CMH11; LVNC4

Reactivity

Human, Mouse, Rat, Monkey, Bovin, Pig, Chicken

Applications

Immunohistochemistry, Western Blot, Immunofluorescence

Purity

Protein G

Pricing

$240/0.02 mL $315/0.05 mL $375/0.1 mL $600/0.2 mL $1,140/2x0.2 mL $1,650/3x0.2 mL $2,135/4x0.2 mL $2,350/5x0.2 mL

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IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human liver tissue using Glycophorin C (GYPC) Rabbit mAb (AAA28458) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using Glycophorin C (GYPC) Rabbit mAb (AAA28458) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using Glycophorin C (GYPC) Rabbit mAb (AAA28458) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human breast tissue using Glycophorin C (GYPC) Rabbit mAb (AAA28458) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human appendix tissue using Glycophorin C (GYPC) Rabbit mAb (AAA28458) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

WB (Western Blot) (Western blot analysis of lysates from K-562 cells, using Glycophorin C (GYPC) Rabbit mAb (AAA28458) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)

WB (Western Blot) (Western blot analysis of various lysates using Glycophorin C (GYPC) Rabbit mAb (AAA28458) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)

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Glycophorin C (GYPC), Monoclonal Antibody (Cat# AAA28458)

Full Name

Glycophorin C (GYPC) Rabbit mAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Purity

Affinity purification

Pricing

$300/0.02 mL $500/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,780/4x0.1 mL $1,960/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of A549 cells with AKT1/2/3 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining AKT1/2/3 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining AKT1/2/3 in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining AKT1/2/3 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-AKT1/2/3 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-AKT1/2/3 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-AKT1/2/3 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-AKT1/2/3 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of AKT1/2/3 on MCF-7 cell lysates using anti-AKT1/2/3 antibody at 1/1, 000 dilution.)

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AKT1/2/3, Monoclonal Antibody (Cat# AAA30101)

Full Name

AKT1/2/3 Antibody

Gene Names

AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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ICC (Immunocytochemistry) (ICC staining Myeloperoxidase in AGS cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Myeloperoxidase in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Myeloperoxidase in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Myeloperoxidase antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Myeloperoxidase antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Myeloperoxidase antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-Myeloperoxidase antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Myeloperoxidase on HL-60 cell lysates using anti-Myeloperoxidase antibody at 1/1, 000 dilution.)

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Myeloperoxidase, Monoclonal Antibody (Cat# AAA30305)

Full Name

Myeloperoxidase Antibody

Reactivity

Human

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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WB (Western Blot) (Anti-GST Tag mouse monoclonal antibody at 1:25000, 1:1250, 1:25000Lane A: GST-mBID transfected E.coli cell lysate (0.2ug)Lane B: GST-YWHAB transfected E.coli cell lysate (0.2ug)Lane C: Empty vecter (0.2ug)SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.)

WB (Western Blot) (Anti-GST Tag mouse monoclonal antibody at 1:25000, 1:1250, 1:1000Lane A: GST-mBID transfected E.coli cell lysate (0.1ug)Lane B: GST-YWHAB transfected E.coli cell lysate (0.1ug)Lane C: Empty vecter (0.1ug)SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.)

Application Data (Recognize GST tag fusion proteins which expressed by E. coli.Anti-GST Tag mouse monoclonal antibody at 1:25000, 1:1250Lane A: GST-mBID transfected E.coli cell lysate (0.5ug)Lane B: GST-mBID transfected E.coli cell lysate (0.2ug)Lane C: GST-mBID transfected E.coli cell lysate (0.1ug)Lane D: GST-mBID transfected E.coli cell lysate (0.05ug)Lane E: GST-mBID transfected E.coli cell lysate (0.02ug)Lane F: GST-YWHAB transfected E.coli cell lysate (0.5ug)Lane G: GST-YWHAB transfected E.coli cell lysate (0.2ug)Lane H: GST-YWHAB transfected E.coli cell lysate (0.1ug)Lane I: GST-YWHAB transfected E.coli cell lysate (0.05ug)Lane J: GST-YWHAB transfected E.coli cell lysate (0.02ug)Lane K: Empty vecter (0.5ug)SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.)

WB (Western Blot) (Recognize GST tag fusion proteins which expressed by 293 cell.Anti-GST Tag mouse monoclonal antibody at 1:1250 or 1:2500Lane A: GST-FABP4-DYKDDDDK transfected 293 Cell Lysate (30ug)Lane B: DYKDDDDK-FABP4-GST transfected 293 Cell Lysate (30ug)Lane C: DYKDDDDK-AGR1-GST transfected 293 Cell Lysate (30ug)Lane D: Negative controlSecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.)

WB (Western Blot) (Recognize GST tag fusion protein human USP7.Anti-GST Tag mouse monoclonal antibody at 1:4000Lane A: GST-USP7 (Recombinant protein) (100ng)Lane B: GST-USP7 (Recombinant protein) (50ng)SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.)

Application Data (Recognize GST tag fusion protein human ERK2.Anti-GST Tag mouse monoclonal antibody at 1:4000Lane A: GST-ERK2 (Recombinant protein) (100ng)Lane B: GST-ERK2 (Recombinant protein) (50ng)SecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique. Performed under reducing conditions.)

IP (Immunoprecipitation) (Anti-GST Tag was immunoprecipitated using:Lane A: GST-ARG1-DYKDDDDK transfected 293 cell lysate (0.5mg)Lane B: DYKDDDDK-ARG1-GST transfected 293 cell lysate (0.5mg) 2 ug anti-HA Tag mouse monoclonal antibody and 60 ug of Immunomagnetic beads Protein G.Primary antibody:Anti-GST Tag rabbit monoclonal antibody, at 1:100 dilutionSecondaryGoat Anti-Mouse IgG H&L (Dylight800) at 1/15000 dilution.Developed using the Odyssey technique.Performed under reducing conditions.)

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GST, Monoclonal Antibody (Cat# AAA27744)

Full Name

Anti-GST tag Antibody, Mouse Monoclonal

Reactivity

Schistosoma japonicum

Applications

Western Blot, Immunoprecipitation

Purity

Protein A

Pricing

$225/0.02 mL $385/0.1 mL $655/0.5 mL $1,245/2x0.5 mL $1,810/3x0.5 mL $2,340/4x0.5 mL $2,655/5x0.5 mL

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Application Data

Application Data

Application Data

Application Data

Application Data

SDS-PAGE (Jurkat whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CD45(ABT-CD45) antibody. The HRP-conjugated Goat anti-M)

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CD45, Monoclonal Antibody (Cat# AAA30872)

Full Name

CD45 (ABT-CD45) Antibody

Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Immunofluorescence

Purity

Protein G

Pricing

$240/0.02 mL $315/0.05 mL $375/0.1 mL $600/0.2 mL $1,140/2x0.2 mL $1,650/3x0.2 mL $2,135/4x0.2 mL $2,350/5x0.2 mL

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Application Data (Publised customer image:Mouse anti Human CD163 antibody, clone EDHu-1 used for the identification of perivascular macrophages in human brain by immunofluorescence.Image caption:Images demonstrating immunohistological stainings of amylin and double immunofluorescence staining against NG2/amylin, laminin/amylin and CD163/amylin in the hippocampus of the patient with AD and T2D.Amylin cell inclusions are indicated with arrows in (a) and shown in a higher magnification in (b). Pericytes with round cell bodies and NG2-positive coverage of the microvessel surface (green in c), without amylin cell inclusions (red in d), displayed round DAPI-positive cell nuclei (blue in e). The images in (c), (d) and (e) are merged in (f). Cells with more diffuse and weak NG2 staining (indicated by the arrowhead, green in g) and cytosolic amylin cell inclusions (red in h) showed altered cell nuclei (indicated with arrow, blue in i).The adjacent unaffected NG2-positive cell is indicated with an arrowhead in (i). The images in (g), (h) and (i) are merged in (j). Cells enclosed by laminin (green in K) contained amylin grains (red in l) and fragmented DAPI-positive cell nuclei (indicated with an arrow blue in m). The images in (k), (l) and (m) are merged in (n).Loss of NG2 coverage (green in o) was associated with polarized amylin cell inclusion (red in p) and fragmented DAPI-positive cell nuclei (indicated with an arrow, blue in q). The images in (o), (p) and (q) are merged in (r). Staining against macrophage marker CD163 (green in s) did not co-localize with amylin cell inclusions (red in t). The cell nucleus was stained with DAPI (blue in U). The images in (s), (t) and (u) are merged in (v). Scale bars: (a) 50 mum, (b), (c) to (v) 5 mum.From: Schultz, N. et al. (2016).Amylin alters human brain pericyte viability and NG2 expression.J Cereb Blood Flow & Metab. Jun 28 [Epub ahead of print]This is from an open access article distributed under the terms of the Creative Commons Attribution License.)

Application Data (Published customer image:Mouse anti Human CD163 antibody, clone EDHu-1 used for the identification of infiltrating macrophages in the pulmonary tissues of cattle by immunohistochemistry on formalin fixed tissue sections.Image caption:Immunohistochemical Labeling of Leukocytes in the Lungs.Shown are the results of IHC labeling for CD3-like immunoreactivity (CD3-li), CD163-li, IBA-1-li, and IL-17-li in the lung of uninfected control and representative infected Holstein calves.Note: CD3-li, CD163-li, and IL-17-li is significantly increased in the lungs of the infected calf.Furthermore, CD3+, CD163+, IBA-1+ and IL-17+ mononuclear cells are components of vasculitis lesions. Vascular endothelial cells consistently exhibit pronounced IL-17-li in the infected calf, but endothelial IL-17-li is rare in the control calf. Scale bar: 200 mum.)

IF (Immunofluorescence) (Immunofluorescence staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1 , red in A and Mouse anti Human CD21 antibody, clone LB21 , green in B. C is the merged image with nuclei counterstained blue using DAPI. High power.)

IF (Immunofluorescence) (Immunofluorescence staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1 , red in A and Mouse anti Human CD21 antibody, clone LB21 , green in B. C is the merged image with nuclei counterstained blue using DAPI. Medium power.)

IF (Immunofluorescence) (Immunofluorescence staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1 , red in A and Mouse anti Human CD21 antibody, clone LB21 , green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power.)

Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1 followed by the Histar detection system . High power.)

Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1 followed by the Histar detection system . Medium power.)

Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD163 antibody, clone EDHu-1 followed by the Histar detection system . Low power.)

Application Data (Figure A. Alexa Fluor700 conjugated mouse anti human CD14 and RPE conjugated mouse IgG1 isotype control . Figure B. Alexa Fluor700 conjugated mouse anti human CD14 and RPE conjugated mouse anti human CD163 . All experiments performed on red cell lysed human blood gated on monocytes in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 cell analyzer.)

Application Data (Figure A. RPE conjugated mouse anti CD14 and FITC conjugated mouse IgG1 isotype control . Figure B. RPE conjugated mouse anti CD14 and FITC conjugated mouse anti human CD163 . All experiments performed on human peripheral blood mononuclear cells in the presence of Human SeroBlock (BUF070A).)

Application Data (Figure A. Alexa Fluor700 conjugated mouse anti human CD14 and Alexa Fluor488 conjugated mouse IgG1 isotype control . Figure B. Alexa Fluor700 conjugated mouse anti human CD14 and Alexa Fluor488 conjugated mouse anti human CD163 . All experiments performed on red cell lysed human blood gated on mononuclear cells in the presence of Human Seroblock (BUF070A). Data acquired on the ZE5 cell analyzer.)

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CD163, Monoclonal Antibody (Cat# AAA12256)

Full Name

Mouse Anti Human CD163: RPE

Gene Names

CD163; M130; MM130; SCARI1

Reactivity

Human

Applications

Flow Cytometry

Pricing

$625/100 Tests $1,190/2x100 Tests $1,720/3x100 Tests $2,225/4x100 Tests $2,490/5x100 Tests

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WB (Western Blot) (ACTB monoclonal antibody Western Blot analysis of ACTB expression in K-562.)

WB (Western Blot) (ACTB monoclonal antibody Western Blot analysis of ACTB expression in NIH/3T3.)

Application Data (Detection limit for recombinant GST tagged ACTB is ~1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ACTB on formalin-fixed paraffin-embedded human smooth muscle. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of ACTB expression in transfected 293T cell line by ACTB monoclonal antibody Lane 1: ACTB transfected lysate (42kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (ACTB monoclonal antibody Western Blot analysis of ACTB expression in Raw 264.7.)

WB (Western Blot) (ACTB monoclonal antibody Western Blot analysis of ACTB expression in HeLa.)

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ACTB, Monoclonal Antibody (Cat# AAA24122)

Full Name

ACTB (Actin, Cytoplasmic 1, Beta-actin) (AP)

Gene Names

ACTB; BRWS1; PS1TP5BP1

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (BANF1 monoclonal antibody (M07), clone M2. Western Blot analysis of BANF1 expression in PC-12.)

WB (Western Blot) (BANF1 monoclonal antibody (M07), clone M2. Western Blot analysis of BANF1 expression in Raw 264.7.)

WB (Western Blot) (BANF1 monoclonal antibody (M07), clone M2. Western Blot analysis of BANF1 expression in Jurkat.)

Application Data (Detection limit for recombinant GST tagged BANF1 is approximately 10ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to BANF1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to BANF1 on HeLa cell. [antibody concentration 10 ug/ml])

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BANF1, Monoclonal Antibody (Cat# AAA25911)

Full Name

BANF1 (Barrier to Autointegration Factor 1, BAF, BCRP1, D14S1460, MGC111161) (AP)

Gene Names

BANF1; BAF; NGPS; BCRP1; D14S1460

Applications

Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MCM3 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MCM3 on HeLa cell. [antibody concentration 10 ug/ml])

Application Data (Detection limit for recombinant GST tagged MCM3 is approximately 0.1ng/ml as a capture antibody.)

WB (Western Blot) (MCM3 monoclonal antibody (M04), clone 2H3. Western Blot analysis of MCM3 expression in Y-79 (Cat # L042V1).)

WB (Western Blot) (MCM3 monoclonal antibody (M04), clone 2H3. Western Blot analysis of MCM3 expression in human ovarian cancer.)

WB (Western Blot) (MCM3 monoclonal antibody (M04), clone 2H3. Western Blot analysis of MCM3 expression in Hela S3 NE (Cat # L013V3).)

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MCM3, Monoclonal Antibody (Cat# AAA26644)

Full Name

MCM3 (Minichromosome Maintenance Complex Component 3, HCC5, MGC1157, P1-MCM3, P1.h, RLFB) (PE)

Gene Names

MCM3; HCC5; P1.h; RLFB; P1-MCM3

Applications

Immunofluorescence, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of A549 cells with Fatty Acid Synthase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining Fatty Acid Synthase in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Fatty Acid Synthase in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Fatty Acid Synthase in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Fatty Acid Synthase in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Fatty Acid Synthase antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Fatty Acid Synthase antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Fatty Acid Synthase antibody. Counter stained with hematoxylin.)

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Fatty Acid Synthase, Monoclonal Antibody (Cat# AAA30258)

Full Name

Fatty Acid Synthase Antibody

Gene Names

FASN; FAS; OA-519; SDR27X1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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Application Data (Published customer image Infiltration of GFP+ BM-cells in infarct and peri-infarct regions. (A-B) Dot plots of viable macrophages/granulocytes (CD11b+CD45high, top right quadrants) and microglia (CD11b+CD45dim, bottom right quadrants) in cortex from BM-chimeric unmanipulated mice and mice exposed to pMCAO. (C) Bar graph showing mean numbers of CD11b+CD45dim microglia and CD11b+CD45high macrophages/granulocytes in BM-chimeric mice 24 hours after pMCAO, subdivided based on expression of GFP (n = 5). Approximately 92% of of the CD45high population were GFP+. (D) Estimation and comparison of mean numbers of CD11b+CD45dim microglia in non-chimeric (n = 10) versus BM-chimeric mice (n = 5) 24 hours after of pMCAO shows significantly fewer CD11b+CD45dim microglial cells in irradiated mice. (E) Overview, showing distribution of infiltrating GFP+ BM-derived cells into infarct (IF) and peri-infarct (P-IF) regions 24 hours after pMCAO. (E-G) By 24 hours, GFP+ single cells (F) and vessel-associated aggregates of GFP+ cells (arrows in G) were observed in infarct and peri-infarct regions. Some of the vessel-associated cells were round, leukocyte-like cells (arrows) while others were elongated cells lining the vasculature (arrow heads in G and in insert). (H) Bar graph showing mean numbers of single GFP+ cells and vessel-associated aggregates of GFP+ cells in ipsi- and contralateral cortex 24 hours after surgery (n = 10). (I-P) Immunohistochemical staining of CD45.1 (I, K), CD45.2 (J, L), IgG2a (M, O) and CD45 (N, P) in ischemic tissue in BM-chimeric (I, J, M, N) and non-chimeric mice (K, L, O, P) 24 hours after pMCAO. N.D, none detected. Scale bars: 200 um (A), 10 um (B, C). 50 um (I-P) *P < 0.05, **P < 0.01, and ***P < 0.001.From: Clausen BH, Lambertsen KL, Babcock AA, Holm TH, Dagnaes-Hansen F, Finsen B. Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice. J Neuroinflammation. 2008 Oct 23;5:46.)

Application Data (Staining of mouse bone marrow with Rat anti Mouse CD11b)

Application Data (Immunoperoxidase staining o mouse lymph node cryosection with Rat anti Mouse CD11b antibody, clone 5C6 followed by horseradish peroxidase conjugated Goat anti Rat IgG as a detection reagent. Low power)

Application Data (Published customer image: Activation of adult primary microglial cells in wild-type and IL-1 KO mice. (A) Primary microglial cells were obtained from young adult wild-type mice. The cells stain with the microglial marker CD11b, but not with the neuronal and astroglial markers, NeuN and GFAP, respectively. A few cells are stained with the oligodendroglial cell marker, MBP. NC (inset) is the primary antibody-free negative control. The microglial cells (n = 3 each group) were stimulated for 24 hours in the presence of the vehicle alone, or supplemented with IFN? or IL-4 in the presence or absence of IL-1beta. Total NO (NOx; B), TNFa (C), arginase specific activity (Arg-1 spe. act.; D) and IGF-1 (E) were determined from the media or cell suspensions. (B) NOx levels increase upon exposure of the cells to IL-1beta and in a synergistic manner upon co-treatment of cells with IL-1beta and IFN?, but not when the cotreatment is with IL-4. (C) TNFa levels increase upon exposure of the cells to IFN?, and further upon co-treatment with IL-1beta. Surprisingly, the co-treatment of the cells with IL-4 and IL-1beta induced the highest TNFa level among the experimental treatments used. (D) Arg1-specific activity increased significantly upon exposure to IL-4 and further increased when IL-4 and IL-1beta were employed together. (E) IGF-1 levels decreased with exposure of the cells to IFN? and increased in response to IL-4. The response was partially inhibited by cotreatment of the cells with IL-1beta. Data are expressed as mean +/- SD (n = 3). *: P < 0.05, **: P < 0.01, ***: P < 0.001 compared with the vehicle-treated group in each genotype (one-way ANOVA followed by Dunnett post-hoc test). ANOVA, analysis of variance; IGF-1, insulin-like growth factor.From: Sato A, Ohtaki H, Tsumuraya T, Song D, Ohara K, Asano M, Iwakura Y, Atsumi T, Shioda S. Interleukin-1 participates in the classical and alternative activation of microglia/macrophages after spinal cord injury. J Neuroinflammation. 2012 Apr 7;9:65. doi: 10.1186/1742-2094-9-65.)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD11b:FITC)

Application Data (Immunoperoxidase staining of mouse lymph node cryosection with Rat anti Mouse CD11b antibody, clone 5C6 followed by horseradish peroxidase conjugated Goat anti Rat IgG as a detection reagent. high power)

Application Data (Published customer image BM-derived GFP+ single cells and vessel-associated cells express CD11b. Fluorescence microscopy for GFP combined with immunofluorescence detection of (A) CD11b, (B) vWF and (C) CD31, 24 hours after pMCAO. (A) Fluorescence detection of GFP and CD11b showed that most GFP+ cells co-expressed CD11b (yellow cells, indicated by arrows), and intermingled with CD11b+ host cells. Note also that a few GFP+ cells did not co-express CD11b (arrow head). Insert shows high magnification of GFP+ cells, some of which co-express CD11b, aggregated around a vessel. (B, C) Fluorescence detection of GFP and the endothelial cell markers vWF (B) and CD31 (C). Inserts show higher magnification of sections of the same vessels. Although there are indications that single vWF+ cells co-express GFP (arrows in B), this could not be reproduced using staining for CD31, and the majority of vWF+ and CD31+ cells showed no co-expression of GFP. Instead, GFP remained confined to round and elongated cells located in the juxtavascular space (insert in C). CD11b+ cells were visualized using Alexa Fluor 568-conjugated goat anti-rat IgG, vWF+ and CD31+ cells using Alexa Fluor 546-conjugated goat anti-rabbit IgG and Alexa Fluor 594-conjugated goat anti-rat IgG, respectively. Scale bars: 20 um (A-C)..From: Clausen BH, Lambertsen KL, Babcock AA, Holm TH, Dagnaes-Hansen F, Finsen B. Interleukin-1beta and tumor necrosis factor-alpha are expressed by different subsets of microglia and macrophages after ischemic stroke in mice. J Neuroinflammation. 2008 Oct 23;5:46.)

Application Data (Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD11b, clone 5C6 , green in A and Rat anti Mouse CD8, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. High power)

Application Data (Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD11b, clone 5C6 , green in A and Rat anti Mouse CD8, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. Low power)

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CD11b, Monoclonal Antibody (Cat# AAA12182)

Full Name

RAT ANTI MOUSE CD11b:FITC

Gene Names

Itgam; CR3; CR3A; MAC1; Cd11b; Ly-40; Mac-1; Mac-1a; CD11b/CD18; F730045J24Rik

Applications

Flow Cytometry

Pricing

$585/0.1 mg $1,110/2x0.1 mg $1,610/3x0.1 mg $2,075/4x0.1 mg $2,295/5x0.1 mg

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WB (Western Blot) (TRIM28 monoclonal antibody Western Blot analysis of TRIM28 expression in Hela NE)

WB (Western Blot) (TRIM28 monoclonal antibody Western Blot analysis of TRIM28 expression in PC-12)

WB (Western Blot) (Western blot analysis of TRIM28 over-expressed 293 cell line, cotransfected with TRIM28 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TRIM28 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TRIM28 on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TRIM28 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of TRIM28 expression in transfected 293T cell line by TRIM28 monoclonal antibody Lane 1: TRIM28 transfected lysate (88.5kD). Lane 2: Non-transfected lysate)

WB (Western Blot) (Western Blot detection against Immunogen (41.69kD).)

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KAP-1, Monoclonal Antibody (Cat# AAA24842)

Full Name

KAP-1 (Transcription Intermediary Factor 1-beta, TIF1-beta, E3 SUMO-protein Ligase TRIM28, KRAB-associated Protein 1, KRAB-interacting Protein 1, KRIP-1, Nuclear Corepressor KAP-1, RING Finger Protein 96, Tripartite Motif-containing Protein 28, TRIM28, KA

Gene Names

TRIM28; KAP1; TF1B; RNF96; TIF1B; PPP1R157

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (TRIM28 monoclonal antibody Western Blot analysis of TRIM28 expression in Hela NE)

WB (Western Blot) (TRIM28 monoclonal antibody Western Blot analysis of TRIM28 expression in PC-12)

WB (Western Blot) (Western blot analysis of TRIM28 over-expressed 293 cell line, cotransfected with TRIM28 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TRIM28 monoclonal antibody GAPDH (36.1kD) used as specificity and loading control.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TRIM28 on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TRIM28 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of TRIM28 expression in transfected 293T cell line by TRIM28 monoclonal antibody Lane 1: TRIM28 transfected lysate (88.5kD). Lane 2: Non-transfected lysate)

WB (Western Blot) (Western Blot detection against Immunogen (41.69kD).)

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KAP-1, Monoclonal Antibody (Cat# AAA25139)

Full Name

KAP-1 (Transcription Intermediary Factor 1-beta, TIF1-beta, E3 SUMO-protein Ligase TRIM28, KRAB-associated Protein 1, KRAB-interacting Protein 1, KRIP-1, Nuclear Corepressor KAP-1, RING Finger Protein 96, Tripartite Motif-containing Protein 28, TRIM28, KA

Gene Names

TRIM28; KAP1; TF1B; RNF96; TIF1B; PPP1R157

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)

Application Data (Published customer image: Post-injury 7,8-dihydroxyflavone treatment increased brain BDNF levels and promoted CREB activation. (A) Bar graphs demonstrating brain derived neurotrophic factor (BDNF) protein concentrations measured by enzyme-linked immunosorbent assay (ELISA) in sham-injured, vehicle-treated, and 20 mg/kg 7,8-dihydroxyflavone (DHF 20)-treated mice at 4 days post-TBI. DHF 20 significantly increased BDNF protein levels in the ipsilateral hemisphere at 4 days post-injury. (B) Bar graphs demonstrating BDNF mRNA expression measured by real -time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) at 1 and 4 days post-injury. DHF 20 significantly increased BDNF mRNA levels in the ipsilateral hemisphere at 4 days post-injury. (C) Western blot analysis of the phospho-CREB level in the ipsilateral hemisphere of sham-injured, vehicle-treated, and DHF 20-treated mice at 1 h, 1 day, and 4 days post-injury. DHF 20 enhanced CREB phosphorylation at 4 days post-injury. (D) Identification of phospho-CREB-positive cells 4 day post-injury in the peri-contusional margin by double immunofluorescence staining. Phospho-CREB is shown in red, and NeuN (neurons), GFAP (astrocytes), or F4/80 (microglia) is shown in green. Co-localization of phospho-CREB with NeuN is shown by yellow labeling. Sections were stained with DAPI (blue) to show all nuclei. Values are mean +/- SEM; *P)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: Pacific Blue)

Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen:Biotin)

Application Data (Frozen mouse spleen stained with A: Rat anti Mouse F4/80 followed by Goat anti Rat IgG:HRP or B: Rat anti Mouse F4/80 preincubated with 2 molar excess of Human anti Idiotypic (HCA154) followed by Goat anti Rat IgG:HRP)

Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen:Low Endotoxin)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: RPE - Alexa Fluor 750)

Application Data (Staining of mouse peritoneal macrophages cells with Rat anti Mouse F4/80 antigen:APC)

Application Data (Immunofluorescence image of mouse small intestine stained with Rat anti Mouse F4/80 , red and Rat anti Mouse CD45 , green; nuclei are stained with DAPI, blue. The image higlights macrophages within the lamina propria)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen:FITC)

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F4/80, Monoclonal Antibody (Cat# AAA12173)

Full Name

RAT ANTI MOUSE F4/80

Gene Names

Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7

Applications

Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Radioimmunoassay, Immunohistochemistry, Western Blot

Pricing

$735/0.25 mg $1,415/2x0.25 mg $2,050/3x0.25 mg $2,655/4x0.25 mg $3,010/5x0.25 mg

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Application Data (Lamin A+C Monoclonal Antibody [JOL2])

IHC (Immunohistchemistry) (IHC using Lamin A/C antibody (IQ332) clone JOL2 on Human colon)

IB (Immunoblot) (Immunoblotting of Hela cells with Lamin A+C Antibody (IQ332) clone JOL2)

IHC (Immunohistochemistry) (IHC using Lamin A/C Antibody (IQ332) clone JOL2 on Human skin)

Application Data ((1:1000) staining in A431(A), (1:10000) staining in HeLa(B), and (1:1000) staining in HepG2(C) cells nuclear lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

FCM (Flow Cytometry) (Blue line: Flow cytometric analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.5% Triton. Primary incubation 1hr (1:100 dilution) followed by Alexa Fluor 488 conjugated goat Anti-mouse IgG (1:1000 dilution). Black line: Anti-Unknown Specificity Isotype control)

IF (Immunofluorescence) (Immunofluorescence analysis of paraformaldehyde fixed HeLa cells, permeabilized with 0.15% Triton. Primary incubation 1hr (1:100 dilution) followed by Alexa Fluor 488 secondary antibody (1:1000 dilution), showing nuclear membrane and nucleoplasm staining. Actin filaments were stained with phalloidin (red) and the nuclear stain is DAPI (blue). Negative control: Mouse IgG1 negative control followed by Alexa Fluor 488 secondary antibody.)

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Lamin A+C, Monoclonal Antibody (Cat# AAA13582)

Full Name

Lamin A+C Monoclonal Antibody [JOL2]

Gene Names

LMNA; FPL; IDC; LFP; CDDC; EMD2; FPLD; HGPS; LDP1; LMN1; LMNC; PRO1; CDCD1; CMD1A; FPLD2; LMNL1; CMT2B1; LGMD1B

Reactivity

Human, African Green Monkey

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Unpurified

Pricing

$940/0.5 mL $1,810/2x0.5 mL $2,630/3x0.5 mL $3,405/4x0.5 mL $3,855/5x0.5 mL

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WB (Western Blot) (LHX5 monoclonal antibody (M05). Western Blot analysis of LHX5 expression in 293.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to LHX5 on HeLa cell. [antibody concentration 10ug/ml].)

WB (Western Blot) (LHX5 monoclonal antibody (M05), Western Blot analysis of LHX5 expression in Jurkat.)

WB (Western Blot) (LHX5 monoclonal antibody. Western Blot analysis of LHX5 expression in Y-79.)

WB (Western Blot) (LHX5 monoclonal antibody. Western Blot analysis of LHX5 expression in MES-SA/Dx5.)

WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)

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LHX5, Monoclonal Antibody (Cat# AAA24074)

Full Name

LHX5 (LIM/Homeobox Protein Lhx5, LIM Homeobox Protein 5, MGC129689)

Reactivity

Human

Applications

Western Blot, Immunofluorescence

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

$820/0.1 mg $1,565/2x0.1 mg $2,270/3x0.1 mg $2,940/4x0.1 mg $3,380/5x0.1 mg

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WB (Western Blot) (PKNOX2 monoclonal antibody. Western Blot analysis of PKNOX2 expression in NIH/3T3.)

WB (Western Blot) (Western blot analysis of PKNOX2 over-expressed 293 cell line, cotransfected with PKNOX2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PKNOX2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PKNOX2 on HeLa cell. [antibody concentration 10ug/ml].)

WB (Western Blot) (Western Blot analysis of PKNOX2 expression in transfected 293T cell line by PKNOX2 monoclonal antibody. Lane 1: PKNOX2 transfected lysate (51.9kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (PKNOX2 monoclonal antibody, Western Blot analysis of PKNOX2 expression in Hela NE.)

WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)

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PKNOX2, Monoclonal Antibody (Cat# AAA25502)

Full Name

PKNOX2 (PREP2, Homeobox Protein PKNOX2, Homeobox Protein PREP-2, PBX/Knotted Homeobox 2, FLJ13074) (HRP)

Gene Names

PKNOX2; PREP2

Reactivity

Human, Mouse

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged GCH1 is ~0.3ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of GCH1 transfected lysate using 127227and Protein A Magnetic Bead and immunoblotted with GCH1 rabbit polyclonal antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase on formalin-fixed paraffin-embedded human lymph node using 127227 (3ug/ml).)

WB (Western Blot) (Western Blot analysis of GCH1 expression in transfected 293T cell line using 127227. Lane 1: GCH1 transfected lysate (27.9kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot analysis of GCH1 using 127227 expression in IMR-32.)

WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)

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GCH1, Monoclonal Antibody (Cat# AAA25700)

Full Name

GCH1 (GTP Cyclohydrolase 1, GTP Cyclohydrolase I, GTP-CH-I, DYT5, GCH) (PE)

Gene Names

GCH1; GCH; DYT5; DYT14; DYT5a; GTPCH1; HPABH4B; GTP-CH-1

Reactivity

Human

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

What are Monoclonal Antibodies?

Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.

This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.

AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.

What Are The Uses of Monoclonal Antibodies

Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.

ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.

Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.

Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.

Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.

How Monoclonal Antibodies Are Used as Medicine?

Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).

Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:

• Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.


• Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.


• Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.

Why Buy Monoclonal Antibodies From Us?

At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.

1. Validated for Versatile Applications

The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).

2. Wide Selection & Specialized Options

We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.

3. High-Quality Proteins

Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.

Frequently Asked Questions

1. Are your monoclonal antibodies validated for specific applications?

Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.

2. How do I choose the right monoclonal antibody for my application?

Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.

3. How quickly can I receive my order?

Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.