Monoclonal Antibodies

Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.

< >

<< Previous Next >>

ICC (Immunocytochemistry) (ICC staining TTC11 in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining TTC11 in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining TTC11 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-TTC11 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human small intestine tissue using anti-TTC11 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TTC11 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of TTC11 on SK-Br-3 cell using anti-TTC11 antibody at 1/2, 000 dilution.)

Compare (5 max)

TTC11, Monoclonal Antibody (Cat# AAA30528)

Full Name

TTC11 Antibody

Gene Names

FIS1; TTC11; CGI-135

Reactivity

Human

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

< >

<< Previous Next >>

FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with NF-kB p65 antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining NF-kB p65 in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining NF-kB p65 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining NF-kB p65 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-NF-kB p65 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-NF-kB p65 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-NF-kB p65 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of NF-kB p65 on hybrid fish (crucian-carp) heart tissue lysate using anti-NF-kB p65 antibody at 1/500 dilution.)

WB (Western Blot) (Western blot analysis of NF-kB p65 on different lysates using anti-NF-kB p65 antibody at 1/1, 000 dilution. Positive control: Lane 1: MCF-7 Lane 2: Hela)

Compare (5 max)

NF-kB, Monoclonal Antibody (Cat# AAA30004)

Full Name

NF-kB p65 Antibody

Gene Names

RELA; p65; NFKB3

Reactivity

Human, Mouse, Zebrafish

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

< >

<< Previous Next >>

ICC (Immunocytochemistry) (ICC staining IKB alpha in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining IKB alpha in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining IKB alpha in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-IKB alpha antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-IKB alpha antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse stomach tissue using anti-IKB alpha antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-IKB alpha antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of IKB alpha on different lysates using anti-IKB alpha antibody at 1/1, 000 dilution. Positive control: Lane 1: PC-12 Lane 2: NIH/3T3 Lane 3: Hela)

Compare (5 max)

IKB alpha, Monoclonal Antibody (Cat# AAA30012)

Full Name

IKB alpha Antibody

Gene Names

NFKBIA; IKBA; MAD-3; NFKBI

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TTF2 on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (TTF2 monoclonal antibody (M06), clone 1E8. Western Blot analysis of TTF2 expression in PC-12.)

WB (Western Blot) (TTF2 monoclonal antibody (M06), clone 1E8. Western Blot analysis of TTF2 expression in Hela S3 NE.)

WB (Western Blot) (TTF2 monoclonal antibody (M06), clone 1E8. Western Blot analysis of TTF2 expression in Y-79.)

WB (Western Blot) (TTF2 monoclonal antibody (M06), clone 1E8 Western Blot analysis of TTF2 expression in NIH/3T3.)

WB (Western Blot) (TTF2 monoclonal antibody (M06), clone 1E8. Western Blot analysis of TTF2 expression in human placenta.)

Compare (5 max)

TTF2, Monoclonal Antibody (Cat# AAA26203)

Full Name

TTF2 (Transcription Termination Factor, RNA Polymerase II, HuF2) (Biotin)

Gene Names

TTF2; HuF2; ZGRF6

Applications

Immunofluorescence, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Detection of human Beta-III-Tubulin by western blot. Samples: Whole cell lysate (10 ug) from SK-N-SH, SK-N-BE(2), HEK293T, A-172, and H1975 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Beta-III-Tubulin recombinant monoclonal antibody (AAA23857 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

WB (Western Blot) (Detection of mouse Beta-III-Tubulin by western blot. Samples: Whole cell lysate (10 ug) from NIH 3T3, CT26, ND7/23, TCMK-1, and B35 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Beta-III-Tubulin recombinant monoclonal antibody (AAA23857 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

IP (Immunoprecipitation) (Detection of human Beta-III-Tubulin by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 5% of IP loaded) from SK-N-SH cells. Antibodies: Rabbit anti-Beta-III-Tubulin recombinant monoclonal antibody (AAA23857 lot 1) used for IP at 20 ul/mg lysate. Beta-III-Tubulin was also immunoprecipitated by another antibody against a different epitope of Beta-III-Tubulin (Antibody 2). For blotting immunoprecipitated Beta-III-Tubulin, AAA23857 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.)

IHC (Immunohistochemistry) (Detection of mouse beta-III Tubulin in mouse cerebellum by IHC. Antibody: Rabbit anti-beta-III Tubulin recombinant monoclonal antibody (AAA23857 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB)

IHC (Immunohistochemistry) (Detection of human beta-III Tubulin in cerebellum by IHC. Antibody: Rabbit anti-beta-III Tubulin recombinant monoclonal antibody (AAA23857 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB)

IHC (Immunohistochemistry) (Detection of human beta-III Tubulin in glioblastoma by IHC. Antibody: Rabbit anti-beta-III Tubulin recombinant monoclonal antibody (AAA23857 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB)

ICC (Immunocytochemistry) (Detection of human beta-III Tubulin in SK-N-BE(2) cells by ICC. Antibody: Rabbit anti-beta-III Tubulin recombinant monoclonal antibody (AAA23857 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB)

Compare (5 max)

beta-III Tubulin, Monoclonal Recombinant Antibody (Cat# AAA23857)

Full Name

Rabbit anti-beta-III Tubulin Recombinant Monoclonal Antibody [BLR185J]

Gene Names

TUBB3; CDCBM; FEOM3; TUBB4; CDCBM1; CFEOM3; beta-4; CFEOM3A

Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry

Purity

Purified

Pricing

$295/0.01 mL $680/0.1 mL $980/5x0.01 mL $1,300/2x0.1 mL $1,890/3x0.1 mL $2,440/4x0.1 mL $2,755/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (F11R monoclonal antibody, Western Blot analysis of F11R expression in HepG2.)

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between PRKCZ and F11R. HeLa cells were stained with PRKCZ rabbit purified polyclonal 1:1200 and F11R mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Application Data (Detection limit for recombinant GST tagged F11R is ~0.1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to F11R on formalin-fixed paraffin-embedded human breast cancer tissue. [antibody concentration 1ug/ml].)

WB (Western Blot) (Western Blot analysis of F11R expression in transfected 293T cell line by F11R monoclonal antibody. Lane 1: F11R transfected lysate (32.6kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (59kD).)

Compare (5 max)

Junctional Adhesion Molecule 1, Monoclonal Antibody (Cat# AAA25138)

Full Name

Junctional Adhesion Molecule 1 (JAM-1, CD321, F11 Receptor, F11R, Junctional Adhesion Molecule A, JAMA, JCAM, KAT, Platelet Adhesion Molecule 1, PAM1, Platelet F11 Receptor, UNQ264/PRO301) (FITC)

Gene Names

F11R; JAM; KAT; JAM1; JAMA; JCAM; CD321; PAM-1

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

FCM (Flow Cytometry) (Flow cytometric analysis of HepG2 cells with TOP1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)

ICC (Immunocytochemistry) (ICC staining TOP1 in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining TOP1 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining TOP1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-TOP1 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of TOP1 on different lysates using anti-TOP1 antibody at 1/1, 000 dilution. Positive control: Lane 1: HepG2 Lane 2: Jurkat Lane 3: MCF-7)

Compare (5 max)

Topoisomerase I, Monoclonal Antibody (Cat# AAA30133)

Full Name

Topoisomerase I Antibody

Gene Names

TOP1; TOPI

Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

< >

<< Previous Next >>

Application Data (Published customer image:Phycoerythrin conjugated Mouse anti Human CD83 antibody, clone HB15e used for the evaluation of CD83 expression on monocyte derived dendritic cells by flow cytometry.Phenotypic characterization of immunogenic and tolerogenic moDC populations by flow cytometry. Monocytes were negatively selected from PBMC using magnetic beads. Immature moDC were generated with IL-4 and GM-CSF for 6 days. 15d-PGJ2 (PGJ2 DC) and dexamethasone plus 1alpha,25-dihydroxyvitamin were added to generate tolerogenic moDC, respectively (PGJ2 DC and Dex/VD3 DC). To generate immunogenic moDC, immature moDC were stimulated for 24 h with LPS, polyI:C and a cytokine cocktail containing TNF-alpha, IL-1beta, IL-6 and PGE2, respectively. The phenotypes of the cells were analyzed by flow cytometry. Live cells were gated according to FSC/SSC. One representative experiment out of three is shown.From: Sprater F, Hovden A-O, Appel S (2012)Expression of ESE-3 Isoforms in Immunogenic and Tolerogenic Human Monocyte-Derived Dendritic Cells.PLoS ONE 7(11): e49577.)

Application Data (Immunofluorescence staining of a human tonsil cryosection with Mouse anti Human CD83 antibody, clone HB15e , red in A and Mouse anti Human CD21 antibody, clone LB21 , green in B. The merged image is in C with nuclei counterstained blue using DAPI. High power)

Application Data (Immunofluorescence staining of a human tonsil cryosection with Mouse anti Human CD83 antibody, clone HB15e , red in A and Mouse anti Human CD21 antibody, clone LB21 , green in B. The merged image is in C with nuclei counterstained blue using DAPI. Low power)

Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD83 antibody, clone HB15e followed by the Histar detection system . High power)

Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD83 antibody, clone HB15e followed by the Histar detection system . Medium power)

Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD83 antibody, clone HB15e followed by the Histar detection system . Low power)

Application Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD83 antibody, clone HB15e followed by the Histar detection system . Low power)

Application Data (Staining of KM-H2 cells with Mouse anti Human CD83: Alexa Fluor 647)

Application Data (Staining of KM-H2 cells with Mouse anti Human CD83)

Application Data (Staining of KM-H2 cell line with Mouse anti Human CD83:FITC)

Compare (5 max)

CD83, Monoclonal Antibody (Cat# AAA12282)

Full Name

MOUSE ANTI HUMAN CD83

Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry, Immunohistochemistry, Immunoprecipitation, Immunofluorescence

Pricing

$440/0.1 mg $840/2x0.1 mg $1,205/3x0.1 mg $1,555/4x0.1 mg $1,680/5x0.1 mg

< >

<< Previous Next >>

Application Data (Published customer image: Increased accumulation of repair-associated macrophages surrounding collaterals in ischemic hind limbs is PAR2-dependent. (A) Stainings of CD206-positive macrophages (green) and SMA-positive vessels (red) in non-ischemic (control) and ischemic (ligated) hind limbs of WT, PAR1-/- and PAR2-/- mice are shown. Nuclei were visualized with DAPI (blue). Arrows indicate single macrophages in the non-ischemic adductor. Quantification of the average number of repair-associated macrophages per vessel is indicated on the right. (B) Correlation between the number of CD206-positive macrophages in the ischemic tissues and the expression of CD11b and (C) CD115 on monocytes. ** p)

Application Data (Published customer image: Western blot analysis to determine type of activation of adult primary microglial cells produced from wild-type and IL-1 KO mice stimulated with IL-4, IL-13 or IL-4/IL-13 with or without IL-1beta. (A) Representative western blotting data of primary microglial cells produced from wild-type (wild) and IL-1 KO mice and exposed for 24 hours to IL-4, IL-13 or IL-4 plus IL-13 (IL-4/IL-13) with or without IL-1beta. Each lane expected to CD206 blotting were applied 7 ug of reduced samples. Non-reduced samples (5 ug) were applied to detect CD206. Densitometric analysis of COX2 (B), Ym1 (C), Arg-1 (D) and CD206 (E) (n = 3 each group). (B) COX2 levels are increased by exposure of cells to IL-1beta and are not influenced by IL-4 or IL-13 alone. The COX2 level was slightly enhanced by IL-1beta and IL-4 co-treatment. (C) Ym1 levels are increased by exposure of cells to IL-4 and IL-4/Il-13 and are synergistically increased by co-treatment with IL-1beta. However, only a low level of Ym1 is seen upon exposure of cells to IL-13, and is significantly less than that seen in response to exposure of cells to IL-4. (D) Arg-1 shows similar levels in response to exposure to IL-4 and IL-4/IL-13; these are synergistically increased by co-treatment with IL-1beta. However, low levels of Arg-1 are seen for exposure of cells to IL-13. (G) CD206 was detected in response to exposure of cells to both IL-4 and IL-4/IL-13 with or without IL-1beta; however, CD206 levels in IL-13-exposed samples were lower than those seen with the other treatments. Data are expressed as mean +/- SD (n = 3). *: P < 0.05, **: P < 0.01, ***: P < 0.001 compared with the IL-4-treated group without IL-1beta for each genotype (one-way ANOVA followed by Dunnett post-hoc test). ANOVA, analysis of variance; arg-1, arginase 1; COX 2, cyclooxygenase 2.From: Sato A, Ohtaki H, Tsumuraya T, Song D, Ohara K, Asano M, Iwakura Y, Atsumi T, Shioda S. Interleukin-1 participates in the classical and alternative activation of microglia/macrophages after spinal cord injury. J Neuroinflammation. 2012 Apr 7;9:65.)

Application Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained in blue using DAPI. Low power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . High power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Low power)

Application Data (Staining of J774 cell line with Rat anti Mouse CD206 following permeabilisation with Leucoperm)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD206: Alexa Fluor 488)

Application Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained in blue using DAPI. High power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Medium power)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD206: Alexa Fluor 647)

Compare (5 max)

CD206, Monoclonal Antibody (Cat# AAA12120)

Full Name

RAT ANTI MOUSE CD206:FITC

Gene Names

Mrc1; MR; CD206; AW259686

Applications

Flow Cytometry

Pricing

$375/0.05 mg $700/2x0.05 mg $1,010/3x0.05 mg $1,305/4x0.05 mg $1,365/5x0.05 mg

< >

<< Previous Next >>

WB (Western Blot) (DLX5 monoclonal antibody. Western Blot analysis of DLX5 expression in NIH/3T3.)

WB (Western Blot) (Western Blot analysis of DLX5 expression in transfected 293T cell line by DLX5 monoclonal antibody (M12). Lane 1: DLX5 transfected lysate (31.5kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (DLX5 monoclonal antibody, Western Blot analysis of DLX5 expression in A-431.)

WB (Western Blot) (DLX5 monoclonal antibody. Western Blot analysis of DLX5 expression in Raw 264.7.)

WB (Western Blot) (DLX5 monoclonal antibody. Western Blot analysis of DLX5 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (35.79kD).)

Compare (5 max)

DLX5, Monoclonal Antibody (Cat# AAA25373)

Full Name

DLX5 (Homeobox Protein DLX-5) (HRP)

Gene Names

DLX5; SHFM1D

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (CDC2 monoclonal antibody (M04), clone 8F1. Western Blot analysis of CDC2 expression in PC-12 (Cat # L012V1).)

WB (Western Blot) (CDC2 monoclonal antibody (M04), clone 8F1. Western Blot analysis of CDC2 expression in NIH/3T3 (Cat # L018V1).)

Application Data (Detection limit for recombinant GST tagged CDC2 is approximately 0.1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CDC2 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 1.5 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CDC2 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 1.5 ug/ml])

WB (Western Blot) (CDC2 monoclonal antibody (M04), clone 8F1 Western Blot analysis of CDC2 expression in Hela S3 NE (Cat # L013V3).)

Compare (5 max)

CDC2, Monoclonal Antibody (Cat# AAA26287)

Full Name

CDC2 (Cell Division Cycle 2, G1 to S and G2 to M, CDC28A, CDK1, DKFZp686L20222, MGC111195) (FITC)

Gene Names

CDK1; CDC2; CDC28A; P34CDC2

Applications

Immunohistochemistry, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

Application Data (Staining of human peripheral blood monocytes with MOUSE ANTI HUMAN CD274:RPE)

Application Data (Staining of human peripheral blood monocytes with C2549-22E4.)

Application Data (Staining of human peripheral blood monocytes with C2549-22E1.)

Application Data (Staining of human peripheral blood monocytes with C2549-22E1.)

Application Data (Jurkat cells were stained with C2549-22E (filled histogram) or isotype control (open histogram) followed by PE-conjugated anti-mouse antibody.)

Application Data (Staining of human peripheral blood monocytes with C2549-22E.)

Compare (5 max)

CD274, Monoclonal Antibody (Cat# AAA26806)

Full Name

CD274 (CD274 Antigen, B7 Homolog 1, B7H1, B7-H1, B7-H, MGC142294, MGC142296, OTTHUMP00000021029, Programmed Cell Death 1 Ligand 1, PDCD1 Ligand 1, PDCD1L1, PDCD1LG1, Programmed Death Ligand 1, PDL1, PD-L1, RGD1566211) (MaxLight 550)

Gene Names

CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1

Reactivity

Human

Applications

Flow Cytometry

Purity

Purified by protein G affinity chromatography from tissue culture supernatant.

Pricing

$830/0.1 mL $1,590/2x0.1 mL $2,305/3x0.1 mL $2,980/4x0.1 mL $3,425/5x0.1 mL

< >

<< Previous Next >>

Application Data (Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD45RA:FITC (MCA88F))

Application Data (Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD45RA:BIOTIN (MCA88B))

Application Data (Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD45RA:AZIDE FREE (MCA88XZ))

Application Data (Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD45RA:ALEXA 647 (MCA88A647))

Application Data (Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD45RA: RPE - Alexa Fluor® 647 (MCA88P647))

IHC (Immunohistochemistry) (Staining of formalin fixed, paraffin embedded human tonsil with Mouse anti human CD45RA (MCAP88))

IHC (Immunohistochemistry) (Staining of formalin fixed, paraffin embedded human tonsil with Mouse anti Human CD45RA (MCAP88))

Compare (5 max)

CD45RA, Monoclonal Antibody (Cat# AAA26770)

Full Name

CD45RA (CD45 Antigen, B220, GP180, Leukocyte Common Antigen, LCA, L-CA, LY5, LY-5, Protein Tyrosine Phosphatase Receptor Type C Polypeptide, PTPRC, T200, T200 Glycoprotein) (PE)

Reactivity

Human, Monkey

Applications

Flow Cytometry, Immunohistochemistry

Purity

Purified by protein G affinity chromatography from tissue culture supernatant.

Pricing

$1,115/0.1 mL $2,145/2x0.1 mL $3,115/3x0.1 mL $4,035/4x0.1 mL $4,710/5x0.1 mL

< >

<< Previous Next >>

Application Data

Application Data

Application Data

Application Data

Application Data

SDS-PAGE (K562 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-Topoisomerase II?(ABT272)antibody. The HRP-conjugated Goat anti-M)

Compare (5 max)

Topoisomerase IIalpha, Monoclonal Antibody (Cat# AAA30909)

Full Name

Topoisomerase IIalpha (ABT272) Antibody

Gene Names

TOP2A; TOP2; TP2A

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, Immunofluorescence

Purity

Protein G

Pricing

$240/0.02 mL $315/0.05 mL $375/0.1 mL $600/0.2 mL $1,140/2x0.2 mL $1,650/3x0.2 mL $2,135/4x0.2 mL $2,350/5x0.2 mL

< >

<< Previous Next >>

ICC (Immunocytochemistry) (Confocal imaging of paraffin-embedded Rat brain tissue using Phospho-JNK1-T183/Y185 + JNK2-T183/Y185 + JNK3-T221/Y223 Rabbit mAb (AAA28616, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.)

ICC (Immunocytochemistry) (Confocal imaging of paraffin-embedded Mouse brain tissue using Phospho-JNK1-T183/Y185 + JNK2-T183/Y185 + JNK3-T221/Y223 Rabbit mAb (AAA28616, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Microwave antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.)

IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using Phospho-JNK1-T183/Y185 + JNK2-T183/Y185 + JNK3-T221/Y223 Rabbit mAb (AAA28616) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Phospho-JNK1-T183/Y185 + JNK2-T183/Y185 + JNK3-T221/Y223 Rabbit mAb (AAA28616) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Phospho-JNK1-T183/Y185 + JNK2-T183/Y185 + JNK3-T221/Y223 Rabbit mAb (AAA28616) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using Phospho-JNK1-T183/Y185 + JNK2-T183/Y185 + JNK3-T221/Y223 Rabbit mAb (AAA28616) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

WB (Western Blot) (Western blot analysis of various lysates, using Phospho-JNK1-T183/Y185 + JNK2-T183/Y185 + JNK3-T221/Y223 Rabbit mAb (AAA28616) at1:2000 dilution. C6 cells were treated by UV at room temperature for 15-30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)

WB (Western Blot) (Western blot analysis of various lysates, using Phospho-JNK1-T183/Y185 + JNK2-T183/Y185 + JNK3-T221/Y223 Rabbit mAb (AAA28616) at1:2000 dilution. 293T and NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)

Compare (5 max)

JNK1-T183/Y185 + JNK2-T183/Y185 + JNK3-T221/Y223, Monoclonal Antibody (Cat# AAA28616)

Full Name

Phospho-JNK1-T183/Y185 + JNK2-T183/Y185 + JNK3-T221/Y223 Rabbit mAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Affinity purification

Pricing

$300/0.02 mL $500/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,780/4x0.1 mL $1,960/5x0.1 mL

< >

<< Previous Next >>

ICC (Immunocytochemistry) (ICC staining Phospho-PKC alpha (T638) in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Phospho-PKC alpha (T638) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Phospho-PKC alpha (T638) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Phospho-PKC alpha (T638) antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Phospho-PKC alpha (T638) antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-PKC alpha (T638) antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Phospho-PKC alpha (T638) on different lysates using anti-Phospho-PKC alpha (T638) antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: 293)

Compare (5 max)

PKC alpha, Monoclonal Antibody (Cat# AAA29785)

Full Name

PKC alpha (Phospho-T638) Antibody

Gene Names

PRKCA; AAG6; PKCA; PRKACA; PKC-alpha

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,015/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Western blot analysis of ARID3A over-expressed 293 cell line, cotransfected with ARID3A Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ARID3A monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged ARID3A is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ARID3A on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ARID3A on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml].)

WB (Western Blot) (ARID3A monoclonal antibody, Western Blot analysis of ARID3A expression in K-562.)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

Compare (5 max)

ARID3A, Monoclonal Antibody (Cat# AAA24434)

Full Name

ARID3A (AT-rich Interactive Domain-containing Protein 3A, ARID Domain-containing Protein 3A, B Cell Regulator of IgH Transcription, Bright, Dead Ringer-like Protein 1, E2F-binding Protein 1, DRIL1, DRIL3, DRX, E2FBP1) APC

Gene Names

ARID3A; DRIL1; DRIL3; BRIGHT; E2FBP1

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (VAX1 monoclonal antibody Western Blot analysis of VAX1 expression in NIH/3T3.)

WB (Western Blot) (Western Blot analysis of VAX1 expression in transfected 293T cell line by VAX1 monoclonal antibody Lane 1: VAX1 transfected lysate (21kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (VAX1 monoclonal antibody Western Blot analysis of VAX1 expression in Raw 264.7.)

WB (Western Blot) (VAX1 monoclonal antibody Western Blot analysis of VAX1 expression in HepG2.)

WB (Western Blot) (VAX1 monoclonal antibody Western Blot analysis of VAX1 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)

Compare (5 max)

VAX1, Monoclonal Antibody (Cat# AAA24700)

Full Name

VAX1 (Ventral Anterior Homeobox 1, MCOPS11) APC

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

FCM (Flow Cytometry) (Flow cytometric analysis of HepG2 cells with Cathepsin L/V/K/H antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining Cathepsin L/V/K/H in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat kidney tissue using anti-Cathepsin L/V/K/H antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Cathepsin L/V/K/H antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Cathepsin L/V/K/H antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Cathepsin L/V/K/H on different cells lysates using anti-Cathepsin L/V/K/H antibody at 1/1, 000 dilution. Positive control: Lane 1: HepG2 Lane 2: A549)

Compare (5 max)

Cathepsin LVKH, Monoclonal Antibody (Cat# AAA30319)

Full Name

Cathepsin LVKH Antibody

Gene Names

CTSL1; MEP; CATL; CTSL

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

< >

<< Previous Next >>

FCM (Flow Cytometry) (Flow cytometric analysis of HepG2 cells with PARK7 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining PARK7 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining PARK7 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue using anti-PARK7 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-PARK7 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-PARK7 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PARK7 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-PARK7 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of PARK7 on different lysates using anti-PARK7 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: Jurkat Lane 3: NIH/3T3)

Compare (5 max)

PARK7/DJ1, Monoclonal Antibody (Cat# AAA30163)

Full Name

PARK7/DJ1 Antibody

Gene Names

PARK7; DJ1; DJ-1

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

< >

<< Previous Next >>

Application Data (Staining of mouse spleen with Rat anti Mouse CD4:RPE)

Application Data (Staining of mouse spleen with Rat anti Mouse CD4:Alexa Fluor ?488)

Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD4:Low Endotoxin)

Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD4:FITC)

Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD4 Alexa Fluor ?647)

Application Data (Published customer image: Intrafollicular location of MZB cells in B6.TC spleens. A. Representative spleen sections from 3 mo old B6.TC (left) and B6 (right) mice stained with Moma-1-FITC, CD1d-PE and B220-PB. The B220+ CD1d+ MZB cells show as bright pink while the other B cells show as blue. The ring of Moma-1+ metallophillic macrophages delineates the MZ inner edge. B. Percentage of CD1d+ B220+ B cells relative to total B220+ B cells outside (MZ) and inside (FO) the Moma-1+ ring in 3 mo and 10 mo old B6 and B6.TC mice. The data show means and standard errors of the mean (SEM) calculated of 4 MZ and FO areas for each mouse. ***: p < 0.001 for t tests. C. Representative spleen sections from 10 mo old B6.TC (left) and B6 (right) mice stained with CD4-FITC, CD1d-PE and B220-PB. Boxed areas show multiple contacts between green B6.TC CD4 T cells and pink MZB cells, but not in the B6 spleen. Original magnification: 200X.From: Zhou et al. BMC Immunology 2011 12:7.)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD4 antibody, clone GK1.5 followed by horseradish peroxidase conjugated Goat anti Rat IgG . Low power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD4 antibody, clone GK1.5 followed by horseradish peroxidase conjugated Goat anti Rat IgG . High power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD4 antibody, clone GK1.5 followed by horseradish peroxidase conjugated Goat anti Rat IgG . Medium power)

Application Data (Staining of mouse spleen with Rat anti Mouse CD4)

Compare (5 max)

CD4, Monoclonal Antibody (Cat# AAA12227)

Full Name

RAT ANTI MOUSE CD4

Gene Names

Cd4; L3T4; Ly-4

Applications

Immunohistochemistry, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Western Blot

Pricing

$440/0.1 mg $840/2x0.1 mg $1,205/3x0.1 mg $1,555/4x0.1 mg $1,680/5x0.1 mg

< >

<< Previous Next >>

WB (Western Blot) (Detection of mouse CD3E by western blot. Samples: Whole cell lysate (10 ug) from BW5147.3, NIH 3T3, TCMK-1, EL4, and CTLL-2 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-CD3E recombinant monoclonal antibody (AAA23852 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds.)

IP (Immunoprecipitation) (Detection of mouse CD3E by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from BW5147.3 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-CD3E recombinant monoclonal antibody (AAA23852 lot 1) used for IP at 12 ul/mg lysate. CD3E was also immunoprecipitated by two antibodies against different epitopes of CD3E . For blotting immunoprecipitated CD3E, AAA23852 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 1 second.)

IHC (Immunohistochemistry) (Detection of mouse CD3E in FFPE mouse spleen by IHC. Antibody: Rabbit anti-CD3E recombinant monoclonal antibody (AAA23852 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

IHC (Immunohistochemistry) (Detection of mouse CD3E in FFPE mouse gut by IHC. Antibody: Rabbit anti-CD3E recombinant monoclonal antibody (AAA23852 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry) (Detection of mouse CD3E in FFPE mouse CTLL-2 cells by ICC. Antibody: Rabbit anti-CD3E recombinant monoclonal antibody (AAA23852 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

FCM (Flow Cytometry) (Detection of mouse CD3 (shaded) in EL4 cells by flow cytometry. Antibody: Rabbit anti-CD3E recombinant monoclonal antibody (AAA23852 lot 1) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)

Compare (5 max)

CD3E, Monoclonal Recombinant Antibody (Cat# AAA23852)

Full Name

Rabbit anti-CD3E Recombinant Monoclonal Antibody [BLR175J]

Gene Names

Cd3e; CD3; T3e; AI504783; CD3epsilon

Reactivity

Mouse

Applications

Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry

Purity

Purified

Pricing

$295/0.01 mL $680/0.1 mL $980/5x0.01 mL $1,300/2x0.1 mL $1,890/3x0.1 mL $2,440/4x0.1 mL $2,755/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (MGC13096 monoclonal antibody. Western Blot analysis of MGC13096 expression in Raw 264.7.)

WB (Western Blot) (MGC13096 monoclonal antibody. Western Blot analysis of MGC13096 expression in NIH/3T3.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MGC13096 on HeLa cell. [antibody concentration 10ug/ml].)

Application Data (Detection limit for recombinant GST tagged MGC13096 is ~0.1ng/ml as a capture antibody.)

WB (Western Blot) (MGC13096 monoclonal antibody. Western Blot analysis of MGC13096 expression in PC-12.)

WB (Western Blot) (MGC13096 monoclonal antibody, Western Blot analysis of MGC13096 expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (37kD).)

Compare (5 max)

PDCD2L, Monoclonal Antibody (Cat# AAA24098)

Full Name

PDCD2L (Programmed Cell Death Protein 2-like, MGC13096)

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunofluorescence

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

$820/0.1 mg $1,565/2x0.1 mg $2,270/3x0.1 mg $2,940/4x0.1 mg $3,380/5x0.1 mg

< >

<< Previous Next >>

Application Data (Staining of human peripheral blood lymphocytes with RAT ANTI HUMAN CD28:RPE)

Application Data (Staining of human peripheral blood lymphocytes with RAT ANTI HUMAN CD28:FITC)

Application Data (Staining of human peripheral blood lymphocytes with RAT ANTI HUMAN CD28: AZIDE FREE)

Application Data (Staining of human peripheral blood lymphocytes with RAT ANTI HUMAN CD28: Alexa Fluor® 647)

Application Data (Staining of human peripheral blood lymphocytes with RAT ANTI HUMAN CD28)

Application Data (MCA709B staining of human peripheral blood lymphocytes)

Compare (5 max)

CD28, Monoclonal Antibody (Cat# AAA26793)

Full Name

CD28 (CD28 Antigen, CD28 Molecule, MGC138290, T cell Antigen CD28, T cell Specific Surface Glycoprotein, T cell Specific Surface Glycoprotein CD28, Tp44) (MaxLight 490)

Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry

Purity

Purified by Protein G Affinity Chromatography

Pricing

$1,015/0.1 mL $1,960/2x0.1 mL $2,850/3x0.1 mL $3,690/4x0.1 mL $4,295/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistchemistry) (AAA31474 at 1/100 staining rat testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-mouse antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (AAA31474 at 1/100 staining rat heart tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-mouse antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (AAA31474 at 1/100 staining mouse testis tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-mouse antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (AAA31474 at 1/100 staining aaaaaa by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-mouse antibody was used as the secondary antibody.)

IHC (Immunohistochemistry) (AAA31474 at 1/100 staining human colorectal cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4 degree C overnight. An HRP conjugated anti-mouse antibody was used as the secondary antibody.)

WB (Western Blot) (Western blot analysis of extracts from various samples, using Annexin A2 Mouse Monoclonal Antibody. Lane 1: 293 cells treated with blocking peptide; Lane 2: 293 cells; Lane 3: mouse kidney tissue. Lane 4: rat heart tissue.)

Compare (5 max)

Ubiquitin, Monoclonal Antibody (Cat# AAA31474)

Full Name

Ubiquitin Mouse Monoclonal Antibody

Reactivity

Human, Mouse, Rat
Prediction: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Peptide ELISA

Purity

Affinity-chromatography.

Pricing

$390/0.1 mL $435/0.2 mL $830/2x0.2 mL $1,190/3x0.2 mL $1,530/4x0.2 mL $1,660/5x0.2 mL

< >

<< Previous Next >>

Application Data (Published Customer Image:Mouse CD31 antibody, clone ER-MP12 used for the demonstration of vasculature in mouse brain by immunofluorescence.Image caption:Inhibition of 2-AG hydrolysis reduces LPS-induced BBB permeability. a, b Fibrinogen levels in b plasma and the a ratio of brain to plasma fibrinogen were assessed by ELISA. n?=?5/7 mice per group. c, d Fluorescent immunostaining in the striatum for fibrinogen (red) and vascular marker (CD31; green) demonstrated leakage of fibrinogen into the brain with vehicle treatment, whereas vascular integrity was preserved when (e, f) MAGL was inhibited. g Extravascular fibrinogen was semi-quantitated in fluorescently labeled sections of the striatum. Bar graphs were plotted with mean?+/-SEM and data analyzed using one-way analysis of variance (ANOVA) with Tukey post-hoc comparisons. n = 5/7 mice per group. Significance is shown as *p?)

Application Data (Rat anti Mouse CD31 antibody, clone ER-MP12 used for the detection of blood vessels in an experimental murine tumor model by immunohistofluorescence.Image caption:Percentage of pixels positive for NG2 in 4T1 (n = 5) and RM11 (n = 4 and n = 6) tumors (A,D) from WT and beta3-KO mice were calculated using immunofluorescent images. No statistical differences in 4T1 (p = 0.90) or RM11 (p = 0.23) were found. Mean +/- SD. Representative images of NG2-staining from both genotypes of 4T1 (B,C) (NG2 green, CD31 red) and RM11 (E,F) (NG2 red) tumors are shown. Scale bars indicate 50 mum.From: Reigstad I, Sortland K, Skogstrand T, Reed RK, Stuhr L.The Effect of Stromal Integrin beta3-Deficiency on Two Different Tumors in Mice.Cancers (Basel). 2016 Jan 12;8(1). pii: E14.)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD31 antibody, clone ER-MP12 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . High power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD31 antibody, clone ER-MP12 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Medium power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD31 antibody, clone ER-MP12 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Low power)

Application Data (Figure A. RPE conjugated rat anti mouse CD45R and FITC conjugated rat IgG2a isotype control Figure B. RPE conjugated rat anti mouse CD45R and Pacific Blue conjugated rat anti mouse CD31 . All experiments performed on red cell lysed murine splenocytes gated on mononuclear cells. Data acquired on the ZE5 cell analyzer.)

Application Data (Figure A. Alexa647 conjugated rat anti mouse CD22 and Alexa488 conjugated Rat IgG2a isotype control . Figure B. Alexa647 conjugated rat anti mouse CD22 and Alexa488 conjugated rat anti mouse CD31 . All experiments performed on red cell lysed murine peripheral blood in the presence of murine SeroBlock (BUF041A).)

Application Data (Figure A. RPE conjugated rat anti mouse CD45R and FITC conjugated rat IgG2a isotype control Figure B. RPE conjugated rat anti mouse CD45R and FITC conjugated rat anti mouse CD31 . All experiments performed on red cell lysed murine splenocytes gated on mononuclear cells. Data acquired on the ZE5 cell analyzer.)

Compare (5 max)

CD31, Monoclonal Antibody (Cat# AAA12258)

Full Name

Rat Anti Mouse CD31: FITC

Gene Names

Pecam1; Cd31; Pecam; C85791; PECAM-1

Reactivity

Mouse

Applications

Flow Cytometry

Purity

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Pricing

$530/0.1 mg $1,005/2x0.1 mg $1,455/3x0.1 mg $1,885/4x0.1 mg $2,070/5x0.1 mg

< >

<< Previous Next >>

Application Data (Staining of mouse spleen cells with Rat anti Mouse CD45 (Ly-5))

Application Data (Staining of mouse spleen cells with Rat anti Mouse CD45 (Ly-5):RPE)

Application Data (Staining of mouse spleen with Rat anti Mouse CD45)

Application Data (Staining of mouse spleen with Rat anti Mouse CD45: RPE)

Application Data (Staining of mouse splenocytes with Rat anti Mouse CD45: FITC)

Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD45 (Ly-5):Alexa Fluor 647)

Compare (5 max)

CD45, Monoclonal Antibody (Cat# AAA11856)

Full Name

RAT ANTI MOUSE CD45:FITC

Gene Names

Ptprc; loc; B220; Cd45; L-CA; Ly-5; T200; CD45R; Lyt-4

Applications

Flow Cytometry

Pricing

$530/0.1 mg $1,005/2x0.1 mg $1,455/3x0.1 mg $1,885/4x0.1 mg $2,070/5x0.1 mg

< >

<< Previous Next >>

Application Data (Detection limit for recombinant GST tagged S100A6 is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to S100A6 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to S100A6 on formalin-fixed paraffin-embedded human stomach carcinoma. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of S100A6 expression in transfected 293T cell line by S100A6 monoclonal antibody. Lane 1: S100A6 transfected lysate (10.2kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (S100A6 monoclonal antibody. Western Blot analysis of S100A6 expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (36.01kD).)

Compare (5 max)

S100A6, Monoclonal Antibody (Cat# AAA24943)

Full Name

S100A6 (Protein S100-A6, S100 Calcium-binding Protein A6, Calcyclin, Prolactin Receptor-associated Protein, PRA, Growth Factor-inducible Protein 2A9, MLN 4, CACY) (Biotin)

Gene Names

S100A6; 2A9; PRA; 5B10; CABP; CACY

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

ICC (Immunocytochemistry) (Confocal imaging of paraffin-embedded Mouse colon tissue using Phospho-STAT3-Y705 Rabbit mAb (AAA28610, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IF staining. Objective: 40x.)

IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Phospho-STAT3-Y705 Rabbit mAb (AAA28610) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse colon using Phospho-STAT3-Y705 Rabbit mAb (AAA28610) at dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat kidney using Phospho-STAT3-Y705 Rabbit mAb (AAA28610) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Phospho-STAT3-Y705 Rabbit mAb (AAA28610) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using Phospho-STAT3-Y705 Rabbit mAb (AAA28610) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

WB (Western Blot) (Western blot analysis of lysates from NIH/3T3 cells, using Phospho-STAT3-Y705 Rabbit mAb (AAA28610) at 1:1000 dilution. NIH/3T3 cells were treated by TNF-? (20 ng/mL) at 37? for 30 minutes.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 180s.)

Compare (5 max)

STAT3-Y705, Monoclonal Antibody (Cat# AAA28610)

Full Name

Phospho-STAT3-Y705 Rabbit mAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Affinity purification

Pricing

$300/0.02 mL $500/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,780/4x0.1 mL $1,960/5x0.1 mL

< >

<< Previous Next >>

FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Phospho-JNK1/2/3 (T183+T183+T221) antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining Phospho-JNK1/2/3 (T183+T183+T221) in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Phospho-JNK1/2/3 (T183+T183+T221) in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Phospho-JNK1/2/3 (T183+T183+T221) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Phospho-JNK1/2/3 (T183+T183+T221) antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Phospho-JNK1/2/3 (T183+T183+T221) antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Phospho-JNK1/2/3 (T183+T183+T221) antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Phospho-JNK1/2/3 (T183+T183+T221) on different lysates using anti-Phospho-JNK1/2/3 (T183+T183+T221) antibody at 1/1, 000 dilution. Positive control: Lane 1: NIH/3T3 cell lysate, treated with Anisomycin Lane 2: NIH/3T3 cell lysate, untreated)

Compare (5 max)

JNK1/2/3, Monoclonal Antibody (Cat# AAA29780)

Full Name

JNK1/2/3 (Phospho-T183+T183+T221) Antibody

Gene Names

MAPK8; JNK; JNK1; PRKM8; SAPK1; JNK-46; JNK1A2; SAPK1c; JNK21B1/2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,015/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with MUC-1 / EMA Monoclonal Antibody (MUC1/520).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with MUC-1 / EMA Monoclonal Antibody (MUC1/520).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Breast Carcinoma stained with MUC-1 / EMA Monoclonal Antibody (MUC1/520).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Endometrial Carcinoma stained with MUC-1 / EMA Monoclonal Antibody (MUC1/520).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Colon Carcinoma stained with MUC-1 / EMA Monoclonal Antibody (MUC1/520).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Ovarian Carcinoma stained with MUC-1 / EMA Monoclonal Antibody (MUC1/520).)

Compare (5 max)

MUC1 / EMA /CD227, Monoclonal Antibody (Cat# AAA13843)

Full Name

MUC1 / EMA /CD227 (Epithelial Marker) Mouse Monoclonal Antibody

Gene Names

MUC1; EMA; MCD; PEM; PUM; KL-6; MAM6; MCKD; PEMT; CD227; H23AG; MCKD1; MUC-1; ADMCKD; ADMCKD1; CA 15-3; MUC-1/X; MUC1/ZD; MUC-1/SEC

Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence, Immunohistochemistry

Pricing

$295/0.02 mg (With BSA & Azide at 0.2mg/mL) $475/0.1 mg (With BSA & Azide at 0.2mg/mL) $475/0.1 mg (Without BSA & Azide at 1mg/mL) $905/2x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,310/3x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,690/4x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,900/5x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,900/5x0.1 mg (Without BSA & Azide at 1mg/mL)

< >

<< Previous Next >>

Application Data (Staining of KG1 lymphocytes with Mouse anti Human CD59:FITC)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD59: Azide Free)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD59:RPE)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD59:Alexa Fluor488 (AAA11916A488))

Application Data (Published customer image: The effect of protease treatment on influenza virion associated host proteins. Purified influenza A/WSN/33 virus was either mock treated or subjected to overnight digestion with subtilisin followed by concentration through a sucrose cushion. 10 ug of mock infected cell lysate (lane 1) or influenza infected cell lysate (lane 2) and 2 ug of untreated influenza virions (lane 3) or protease treated influenza virions (lane 4) were then analyzed by western blot with antibodies against the indicated proteins. Numbers to the right are molecular weight markers.From: Shaw ML, Stone KL, Colangelo CM, Gulcicek EE, Palese P (2008) Cellular Proteins in Influenza Virus Particles. PLoS Pathog 4(6): e1000085.)

Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD59:Alexa Fluor 647 (AAA11916A647))

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD59)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD59)

Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD59:Biotin)

Compare (5 max)

CD59, Monoclonal Antibody (Cat# AAA11916)

Full Name

MOUSE ANTI HUMAN CD59

Gene Names

CD59; 1F5; EJ16; EJ30; EL32; G344; MIN1; MIN2; MIN3; MIRL; HRF20; MACIF; MEM43; MIC11; MSK21; 16.3A5; HRF-20; MAC-IP; p18-20

Applications

Immunohistochemistry, Fluorescence Microscopy, Flow Cytometry, Immunofluorescence, Immunoprecipitation, Immunohistochemistry, Western Blot

Pricing

$600/0.2 mg $1,140/2x0.2 mg $1,650/3x0.2 mg $2,135/4x0.2 mg $2,385/5x0.2 mg

< >

<< Previous Next >>

Application Data (Detection limit for recombinant GST tagged PLXNA2 is 0.1ng/ml as a capture antibody.)

WB (Western Blot) (Western Blot analysis of PLXNA2 expression in transfected 293T cell line by PLXNA2 monoclonal antibody. Lane 1: PLXNA2 transfected lysate (Predicted MW: 18.8kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (PLXNA2 monoclonal antibody. Western Blot analysis of PLXNA2 expression in NIH/3T3.)

WB (Western Blot) (PLXNA2 monoclonal antibody. Western Blot analysis of PLXNA2 expression in Raw 264.7.)

WB (Western Blot) (PLXNA2 monoclonal antibody. Western Blot analysis of PLXNA2 expression in HepG2)

WB (Western Blot) (PLXNA2 monoclonal antibody. Western Blot analysis of PLXNA2 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (43.78kD).)

Compare (5 max)

PLXNA2, Monoclonal Antibody (Cat# AAA25210)

Full Name

PLXNA2 (Plexin A2, KIAA0463, OCT, PLXN2, Plexin-A2, Semaphorin Receptor OCT, FLJ11751, FLJ30634) (FITC)

Gene Names

PLXNA2; OCT; PLXN2

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with MUC2 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining MUC2 in SW480 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining MUC2 in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining MUC2 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-MUC2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat skeletal muscle tissue using anti-MUC2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat intestine tissue using anti-MUC2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MUC2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-MUC2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast cancer tissue using anti-MUC2 antibody. Counter stained with hematoxylin.)

Compare (5 max)

MUC2, Monoclonal Antibody (Cat# AAA30338)

Full Name

MUC2 Antibody

Gene Names

MUC2; MLP; SMUC; MUC-2

Reactivity

Human, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

< >

<< Previous Next >>

Application Data (Detection limit for recombinant GST tagged NME1 is approximately 0.1ng/ml as a capture antibody.)

WB (Western Blot) (NME1 monoclonal antibody (M02), clone 1D7. Western Blot analysis of NME1 expression in different cell lines.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NME1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NME1 on HeLa cell. [antibody concentration 10 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NME1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to NME1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3 ug/ml])

Compare (5 max)

NME1, Monoclonal Antibody (Cat# AAA26300)

Full Name

NME1 (Non-Metastatic Cells 1, Protein (NM23A) Expressed in, AWD, GAAD, NB, NBS, NDPK-A, NDPKA, NM23, NM23-H1) (FITC)

Gene Names

NME1; NB; AWD; NBS; GAAD; NDKA; NM23; NDPKA; NDPK-A; NM23-H1

Applications

IF, IHC, IP, WB

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Detection of human gamma-H2AX by western blot. Samples: Whole cell lysate (50 ug) from Jurkat cells treated with 100 uM etoposide (+) or mock treated (-). Antibody: Rabbit anti-gamma-H2AX recombinant monoclonal antibody (AAA23813 lot 2) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds.)

IP (Immunoprecipitation) (Detection of human Gamma-H2AX by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from Jurkat cells treated with 100 uM etoposide or mock treated (-). Antibodies: Rabbit anti-Gamma-H2AX recombinant monoclonal antibody (AAA23813 lot 2) used for IP at 20 ul/mg lysate. H2AX was also immunoprecipitated by goat anti-H2AX antibody . For blotting immunoprecipitated Gamma-H2AX, AAA23813 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds. Lower Panel: goat anti-H2AX antibody .)

IHC (Immunohistochemistry) (Detection of human gamma-H2AX by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Rabbit anti-gamma-H2AX recombinant monoclonal antibody (AAA23813) used at 1:100. Secondary: DyLight 594-conjugated goat anti-rabbit IgG . Counterstain: DAPI.)

IHC (Immunohistochemistry) (Detection of mouse gamma-H2AX by immunohistochemistry. Sample: FFPE section of mouse CT26 tumor. Antibody: Rabbit anti-gamma-H2AX recombinant monoclonal antibody (AAA23813 lot 2) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate:DAB.)

IHC (Immunohistochemistry) (Detection of human gamma-H2AX by immunohistochemistry. Sample: FFPE section of human basal cell carcinoma. Antibody: Rabbit anti-gamma-H2AX recombinant monoclonal antibody (AAA23813 lot 2) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate:DAB.)

ICC (Immunocytochemistry) (Detection of human gamma-H2AX by immunocytochemistry. Sample: FFPE section of human HeLa cells treated with etoposide. Antibody: Rabbit anti-gamma-H2AX recombinant monoclonal antibody (AAA23813 lot 2) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate:DAB.)

FCM (Flow Cytometry) (Detection of human gamma H2AX (shaded) in etoposide treated HEK293T cells by flow cytometry. Antibody: Rabbit anti-gamma H2AX recombinant monoclonal (AAA23813) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)

Compare (5 max)

gamma-H2AX, Monoclonal Recombinant Antibody (Cat# AAA23813)

Full Name

Rabbit anti-gamma-H2AX Recombinant Monoclonal Antibody [BLR053F]

Gene Names

H2AFX; H2AX; H2A.X; H2A/X

Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Immunohistochemistry, Flow Cytometry

Purity

Purified

Pricing

$295/0.01 mL $680/0.1 mL $980/5x0.01 mL $1,300/2x0.1 mL $1,890/3x0.1 mL $2,440/4x0.1 mL $2,755/5x0.1 mL

< >

<< Previous Next >>

Application Data (Proximity Ligation Analysis of protein-protein interactions between TP53 and PML HeLa cells were stained with anti-TP53 rabbit purified polyclonal 1:1200 and anti-PML mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)

Application Data (Detection limit for recombinant GST tagged PML is ~0.3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PML on HeLa cell. [antibody concentration 10ug/ml])

WB (Western Blot) (PML monoclonal antibody Western Blot analysis of PML expression in Hela NE.)

WB (Western Blot) (PML monoclonal antibody Western Blot analysis of PML expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (36.63kD).)

Compare (5 max)

PML, Monoclonal Antibody (Cat# AAA25211)

Full Name

PML (Probable Transcription Factor PML, RING Finger Protein 71, Tripartite Motif-containing Protein 19, PML, MYL, RNF71, TRIM19) (FITC)

Gene Names

PML; MYL; RNF71; PP8675; TRIM19

Reactivity

Human, Rat

Applications

Immunofluorescence, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (BACE1 monoclonal antibody (M02), clone 2C1. Western Blot analysis of BACE1 expression in rat testis.)

WB (Western Blot) (Western Blot analysis of BACE1 expression in transfected 293T cell line by BACE1 monoclonal antibody (M01), clone 2C1.Lane 1: BACE1 transfected lysate(52.9 KDa).Lane 2: Non-transfected lysate.)

Application Data (Proximity Ligation Analysis of protein-protein interactions between MMP2 and BACE1. HeLa cells were stained with anti-MMP2 rabbit purified polyclonal 1:1200 and anti-BACE1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)

Application Data (Detection limit for recombinant GST tagged BACE1 is approximately 0.03ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to BACE1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to BACE1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml])

Compare (5 max)

BACE1, Monoclonal Antibody (Cat# AAA26152)

Full Name

BACE1 (beta-site APP-cleaving Enzyme 1, ASP2, BACE, FLJ90568, HSPC104, KIAA1149) (APC)

Gene Names

BACE1; ASP2; BACE; HSPC104

Applications

Immunohistochemistry, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using [KO Validated] CDKN1A/p21 Rabbit mAb (AAA28483) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using [KO Validated] CDKN1A/p21 Rabbit mAb (AAA28483) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma tissue using [KO Validated] CDKN1A/p21 Rabbit mAb (AAA28483) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)

IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Human liver cancer tissue using [KO Validated] CDKN1A/p21 Rabbit mAb (AAA28483) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon tissue using [KO Validated] CDKN1A/p21 Rabbit mAb (AAA28483) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using [KO Validated] CDKN1A/p21 Rabbit mAb (AAA28483) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)

WB (Western Blot) (Western blot analysis of lysates from wild type(WT) and CDKN1A/p21 knockout (KO) 293T cells, using [KO Validated] CDKN1A/p21 Rabbit mAb (AAA28483) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 90s.)

WB (Western Blot) (Western blot analysis of various lysates using [KO Validated] CDKN1A/p21 Rabbit mAb (AAA28483) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 90s.)

WB (Western Blot) (Western blot analysis of various lysates using [KO Validated] CDKN1A/p21 Rabbit mAb (AAA28483) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 3min.)

Compare (5 max)

CDKN1A/p21, Monoclonal Antibody (Cat# AAA28483)

Full Name

[KO Validated] CDKN1A/p21 Rabbit mAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Purity

Affinity purification

Pricing

$300/0.02 mL $500/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,780/4x0.1 mL $1,960/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat heart using PARP1 Rabbit mAb (AAA28515) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat brain using PARP1 Rabbit mAb (AAA28515) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse testis using PARP1 Rabbit mAb (AAA28515) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse kidney using PARP1 Rabbit mAb (AAA28515) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse colon using PARP1 Rabbit mAb (AAA28515) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse brain using PARP1 Rabbit mAb (AAA28515) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human tonsil using PARP1 Rabbit mAb (AAA28515) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Human spleen using PARP1 Rabbit mAb (AAA28515) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human placenta using PARP1 Rabbit mAb (AAA28515) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human liver cancer using PARP1 Rabbit mAb (AAA28515) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon using PARP1 Rabbit mAb (AAA28515) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

WB (Western Blot) (Western blot analysis of various lysates using PARP1 Rabbit mAb (AAA28515) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)

WB (Western Blot) (Western blot analysis of lysates from Jurkat cells, using PARP1 Rabbit mAb (AAA28515) at 1:1000 dilution. Jurkat cells were treated by Staurosporine(1uM) at room temperature for 3 hours .Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)

Compare (5 max)

PARP1, Monoclonal Antibody (Cat# AAA28515)

Full Name

PARP1 Rabbit mAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Affinity purification

Pricing

$300/0.02 mL $500/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,780/4x0.1 mL $1,960/5x0.1 mL

< >

<< Previous Next >>

ICC (Immunocytochemistry) (ICC staining NFkB p100 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining NFkB p100 in A431 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-NFkB p100 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human placenta tissue using anti-NFkB p100 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NFkB p100 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of NFkB p100 on Hela (1) and MCF-7 (2) cell using anti-NFkB p100 antibody at 1/1, 000 dilution.)

Compare (5 max)

NFkB, Monoclonal Antibody (Cat# AAA30371)

Full Name

NFkB p100 Antibody

Gene Names

NFKB2; p52; p105; H2TF1; LYT10; LYT-10; NF-kB2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

< >

<< Previous Next >>

FCM (Flow Cytometry) (Flow cytometric analysis of N2A cells with Tyrosine Hydroxylase antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as secondary antibody)

ICC (Immunocytochemistry) (ICC staining Tyrosine Hydroxylase in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Tyrosine Hydroxylase in NIH/3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Tyrosine Hydroxylase in N2A cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-Tyrosine Hydroxylase antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Tyrosine Hydroxylase on PC-12 cells lysates using anti-Tyrosine Hydroxylase antibody at 1/1, 000 dilution.)

Compare (5 max)

Tyrosine Hydroxylase, Monoclonal Antibody (Cat# AAA30154)

Full Name

Tyrosine Hydroxylase Antibody

Gene Names

TH; TYH; DYT14; DYT5b

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

< >

<< Previous Next >>

FCM (Flow Cytometry) (Flow cytometric analysis of Jurkat cells with NFAT1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining NFAT1 in SHG-44 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining NFAT1 in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining NFAT1 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-NFAT1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NFAT1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-NFAT1 antibody. Counter stained with hematoxylin.)

Compare (5 max)

NFAT1, Monoclonal Antibody (Cat# AAA30340)

Full Name

NFAT1 Antibody

Gene Names

NFATC2; NFAT1; NFATP

Reactivity

Human

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

< >

<< Previous Next >>

ELISA (Titration curve analysis of B7-H3 mAbs to detect recombinant B7-H3 in ELISA at decreasing concentrations.)

FCM (Flow Cytometry) (Flow cytometry analysis of B7-H3 in HEK293 cells using B7-H3 antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: B7-H3 over expressing HEK293 cells.)

IHC (Immunohistochemistry) (Immunohistochemistry of B7-H3 in human colon carcinoma tissue using B7-H3 Antibody and control mouse IgG (corner box) at 2 μg/ml.)

IF (Immunofluorescence) (Immunofluorescence of B7-H3 in human colon carcinoma tissue cells using B7-H3 Antibody at 10 μg/ml.Green: B7-H3 Antibody [10G6]Blue: DAPI staining)

IF (Immunofluorescence) (Immunofluorescence of B7-H3 in HEK293 cells using B7-H3 Antibody at 5 μg/ml.Green: B7-H3 Antibody [10G6]Blue: DAPI staining)

ICC (Immunocytochemistry) (Immunocytochemistry of B7-H3 in HEK293 cells using B7-H3 antibody and control mouse IgG antibody (left corner box) at 1 μg/ml.)

Compare (5 max)

B7-H3, Monoclonal Antibody (Cat# AAA11026)

Full Name

B7-H3 Antibody [10G6]

Gene Names

CD276; B7H3; B7-H3; B7RP-2; 4Ig-B7-H3

Reactivity

Human

Applications

Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Protein A purified

Pricing

$570/0.1 mg $1,080/2x0.1 mg $1,560/3x0.1 mg $2,015/4x0.1 mg $2,295/5x0.1 mg

< >

<< Previous Next >>

Application Data (Proximity Ligation Analysis of protein-protein interactions between STAT1 and PDGFRB Mahlavu cells were stained with anti-STAT1 rabbit purified polyclonal (1:1200) and 131071 (1:50). Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)

Application Data (Detection limit for recombinant GST tagged PDGFRB is ~1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of PDGFRB transfected lysate using 131071 and Protein A Magnetic Bead, and immunoblotted with PDGFRB rabbit polyclonal antibody.)

WB (Western Blot) (Western Blot analysis of PDGFRB expression in human uterus myoma using 131071.)

WB (Western Blot) (Western Blot analysis of PDGFRB expression in human stomach using 131071.)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

Compare (5 max)

PDGFRB, Monoclonal Antibody (Cat# AAA26698)

Full Name

PDGFRB (PDGFR, PDGFR1, Platelet-derived Growth Factor Receptor beta, Beta Platelet-derived Growth Factor Receptor, Beta-type Platelet-derived Growth Factor Receptor, CD140 Antigen-like Family Member B, Platelet-derived Growth Factor Receptor 1, CD140b) (M

Gene Names

PDGFRB; IMF1; KOGS; IBGC4; JTK12; PDGFR; PENTT; CD140B; PDGFR1; PDGFR-1

Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

ICC (Immunocytochemistry) (ICC staining PSMA1 in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining PSMA1 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining PSMA1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-PSMA1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue using anti-PSMA1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat testis tissue using anti-PSMA1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-PSMA1 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of PSMA1 on different lysates using anti-PSMA1 antibody at 1/500 dilution. Positive control: Lane 1: PC-12 Lane 2: Mouse spleen tissue Lane 3: Rat spleen tissue)

WB (Western Blot) (Western blot analysis of PSMA1 on different cell lysates using anti-PSMA1 antibody at 1/500 dilution. Positive control: Lane 1: Jurkat Lane 2: Hela Lane 3: HepG2 Lane 4: 293)

Compare (5 max)

PSMA1, Monoclonal Antibody (Cat# AAA30400)

Full Name

PSMA1 Antibody

Gene Names

PSMA1; NU; HC2; PROS30; HEL-S-275

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

< >

<< Previous Next >>

FCM (Flow Cytometry) (Flow cytometric analysis of HeLa cells with beta-tubulin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Goat anti mouse IgG (FITC) was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining��-tubulin in NIH/3T3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC stainingbeta-tubulin in HepG2 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining beta-tubulin in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-beta-tubulin antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of beta-tubulin on different cell lysates using anti-beta-tubulin antibody at 1/5000 dilution. Positive control: Lane 1: NCCIT Lane 2: NIH/3T3 Lane 3: PC12 Lane 4: Mouse heart Lane 5: F9 Lane 6: zebrafish brain Lane 7: Hela)

Compare (5 max)

beta-Tubulin, Monoclonal Antibody (Cat# AAA29888)

Full Name

beta-Tubulin Antibody

Gene Names

TUBB; M40; TUBB1; TUBB5; OK/SW-cl.56

Reactivity

Human, Mouse, Rat, Zebrafish

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,015/5x0.1 mL

< >

<< Previous Next >>

FCM (Flow Cytometry) (Flow cytometric analysis of MCF-7 cells with Peroxiredoxin 3 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)

ICC (Immunocytochemistry) (ICC staining Peroxiredoxin 3 in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Peroxiredoxin 3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Peroxiredoxin 3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti- Peroxiredoxin 3 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti- Peroxiredoxin 3 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti- Peroxiredoxin 3 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Peroxiredoxin 3 on different cell lysate using anti-Peroxiredoxin 3 antibody at 1/1, 000 dilution. Positive control�� Lane1: Human liver Lane2: MCF-7 Lane3: A431)

Compare (5 max)

Peroxiredoxin 3, Monoclonal Antibody (Cat# AAA30363)

Full Name

Peroxiredoxin 3 Antibody

Gene Names

PRDX3; AOP1; MER5; AOP-1; SP-22; HBC189; PRO1748; prx-III

Reactivity

Human

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Immunoprecipitation, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

< >

<< Previous Next >>

Application Data (Proximity Ligation Analysis of protein-protein interactions between PRKCZ and AKT3. HeLa cells were stained with anti-PRKCZ rabbit purified polyclonal 1:1200 and anti-AKT3 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)

Application Data (Detection limit for recombinant GST tagged AKT3 is ~0.03ng/ml as a capture antibody.)

WB (Western Blot) (AKT3 monoclonal antibody Western Blot analysis of AKT3 expression in MCF-7.)

WB (Western Blot) (AKT3 monoclonal antibody Western Blot analysis of AKT3 expression in Raw 264.7.)

WB (Western Blot) (AKT3 monoclonal antibody Western Blot analysis of AKT3 expression in PC-12.)

WB (Western Blot) (AKT3 monoclonal antibody Western Blot analysis of AKT3 expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (35.53kD).)

Compare (5 max)

AKT3, Monoclonal Antibody (Cat# AAA24429)

Full Name

AKT3 (RAC-gamma Serine/Threonine-protein Kinase, Protein Kinase Akt-3, Protein Kinase B gamma, PKB gamma, RAC-PK-gamma, STK-2, PKBG) APC

Gene Names

AKT3; MPPH; PKBG; MPPH2; PRKBG; STK-2; PKB-GAMMA; RAC-gamma; RAC-PK-gamma

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

< >

<< Previous Next >>

Application Data (Detection limit for recombinant GST tagged SMAD3 is approximately 3ng/ml as a capture antibody.)

WB (Western Blot) (SMAD3 monoclonal antibody (M09), clone 1C11. Western Blot analysis of SMAD3 expression in SJCRH30.)

WB (Western Blot) (SMAD3 monoclonal antibody (M09), clone 1C11. Western Blot analysis of SMAD3 expression in Jurkat.)

WB (Western Blot) (SMAD3 monoclonal antibody (M09), clone 1C11 Western Blot analysis of SMAD3 expression in Hela S3 NE.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SMAD3 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to SMAD3 on HeLa cell. [antibody concentration 10 ug/ml])

Compare (5 max)

SMAD3, Monoclonal Antibody (Cat# AAA26164)

Full Name

SMAD3 (SMAD Family Member 3, DKFZp586N0721, DKFZp686J10186, HSPC193, HsT17436, JV15-2, MADH3, MGC60396) (Biotin)

Gene Names

SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436

Applications

Immunofluorescence, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

What are Monoclonal Antibodies?

Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.

This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.

AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.

What Are The Uses of Monoclonal Antibodies

Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.

ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.

Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.

Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.

Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.

How Monoclonal Antibodies Are Used as Medicine?

Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).

Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:

• Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.


• Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.


• Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.

Why Buy Monoclonal Antibodies From Us?

At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.

1. Validated for Versatile Applications

The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).

2. Wide Selection & Specialized Options

We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.

3. High-Quality Proteins

Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.

Frequently Asked Questions

1. Are your monoclonal antibodies validated for specific applications?

Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.

2. How do I choose the right monoclonal antibody for my application?

Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.

3. How quickly can I receive my order?

Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.