Monoclonal Antibodies

Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.

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ELISA (Titration curve analysis of B7-H3 mAbs to detect recombinant B7-H3 in ELISA at decreasing concentrations.)

FCM (Flow Cytometry) (Flow cytometry analysis of B7-H3 in HEK293 cells using B7-H3 antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: B7-H3 over expressing HEK293 cells.)

IHC (Immunohistochemistry) (Immunohistochemistry of B7-H3 in human colon carcinoma tissue using B7-H3 Antibody and control mouse IgG (corner box) at 2 μg/ml.)

IF (Immunofluorescence) (Immunofluorescence of B7-H3 in human colon carcinoma tissue cells using B7-H3 Antibody at 10 μg/ml.Green: B7-H3 Antibody [2A7]Blue: DAPI staining)

IF (Immunofluorescence) (Immunofluorescence of B7-H3 in HEK293 cells using B7-H3 Antibody at 5 μg/ml.Green: B7-H3 Antibody [2A7]Blue: DAPI staining)

ICC (Immunocytochemistry) (Immunocytochemistry of B7-H3 in HEK293 cells using B7-H3 antibody and control mouse IgG antibody (left corner box) at 1 μg/ml.)

WB (Western Blot) (Western blot analysis of B7-H3 in HEK293 cells using B7-H3 antibody at (A) 0.25 (B) 0.5 and (C) 1 μg/ml.)

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B7-H3, Monoclonal Antibody (Cat# AAA11023)

Full Name

B7-H3 Antibody [2A7]

Gene Names

CD276; B7H3; B7-H3; B7RP-2; 4Ig-B7-H3

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Protein A purified

Pricing

$570/0.1 mg $1,080/2x0.1 mg $1,560/3x0.1 mg $2,015/4x0.1 mg $2,295/5x0.1 mg

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IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human brain tissues using CYP3A4 mouse mAb with DAB staining.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded liver cancer tissues using CYP3A4 mouse mAb with DAB staining.)

FCM (Flow Cytometry) (Flow cytometric analysis of HepG2 cells using CYP3A4 mouse mAb (green) and negative control (red).)

IF (Immunofluorescence) (Immunofluorescence analysis of HepG2 cells using CYP3A4 mouse mAb (green). Blue)

WB (Western Blot) (Western blot analysis using CYP3A4 mAb against HEK293 (1) and CYP3A4 (AA)

WB (Western Blot) (Western blot analysis using CYP3A4 mAb against human CYP3A4 (AA)

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CYP3A4, Monoclonal Antibody (Cat# AAA14115)

Full Name

Anti-CYP3A4 Mouse mAb

Gene Names

CYP3A4; HLP; CP33; CP34; CYP3A; NF-25; CYP3A3; P450C3; CYPIIIA3; CYPIIIA4; P450PCN1

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry

Pricing

$565/0.1 mL $1,070/2x0.1 mL $1,545/3x0.1 mL $1,900/5x0.1 mL $1,995/4x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CAND1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CAND1 on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (CAND1 monoclonal antibody (M05), clone 1G5. Western Blot analysis of CAND1 expression in Raw 264.7.)

WB (Western Blot) (CAND1 monoclonal antibody (M05), clone 1G5. Western Blot analysis of CAND1 expression in PC-12.)

WB (Western Blot) (CAND1 monoclonal antibody (M05), clone 1G5. Western Blot analysis of CAND1 expression in NIH/3T3.)

WB (Western Blot) (CAND1 monoclonal antibody (M05), clone 1G5. Western Blot analysis of CAND1 expression in Hela S3 NE (Cat # L013V3).)

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CAND1, Monoclonal Antibody (Cat# AAA26649)

Full Name

CAND1 (Cullin-Associated and Neddylation-dissociated 1, DKFZp434M1414, FLJ10114, FLJ10929, FLJ38691, FLJ90441, KIAA0829, TIP120, TIP120A) (PE)

Gene Names

CAND1; TIP120; TIP120A

Applications

Immunofluorescence, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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ICC (Immunocytochemistry) (ICC staining Ku80 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Ku80 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Ku80 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Ku80 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Ku80 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kideny tissue using anti-Ku80 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Ku80 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Ku80 on MCF-7 cells lysates using anti-Ku80 antibody at 1/1, 000 dilution.)

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Ku80, Monoclonal Antibody (Cat# AAA30120)

Full Name

Ku80 Antibody

Gene Names

XRCC5; KU80; KUB2; Ku86; NFIV; KARP1; KARP-1

Reactivity

Human

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of MCF-7 cells with TTR antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining TTR in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining TTR in A549 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-TTR antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-TTR antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human thyroid tissue using anti-TTR antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti- TTR antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-TTR antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of TTR on different cells lysates using anti-TTR antibody at 1/500 dilution. Positive control�� Line 1: Human brain Line 2��Human lung)

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TTR, Monoclonal Antibody (Cat# AAA30335)

Full Name

TTR Antibody

Gene Names

TTR; CTS; CTS1; PALB; TBPA; HsT2651

Reactivity

Human

Applications

Western Blot, Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometry analysis of TIM-3 overexpressing HEK293 cells using TIM-3 antibody and control mouse IgG antibody at 0.1 μg/ml. Blue: Untransfected HEK293 cells. Yellow: TIM-3 overexpressing HEK293 cells.)

IHC (Immunohistochemistry) (Immunohistochemistry of TIM-3 in human colon carcinoma tissue with TIM-3 antibody at 5 μg/mL.)

IF (Immunofluorescence) (Immunofluorescence of TIM-3 in human colon carcinoma tissue with TIM-3 antibody at 20 μg/mL.Green: TIM-3 Antibody [2A6]Blue: DAPI staining)

IF (Immunofluorescence) (Immunofluorescence of TIM-3 in transfected HEK293 cells with TIM-3 antibody at 10 μg/mL.Green: TIM-3 Antibody [2A6]Blue: DAPI staining)

ICC (Immunocytochemistry) (Immunocytochemistry of TIM-3 in transfected HEK293 cells with TIM-3 antibody at 1 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 1 μg/mL.)

WB (Western Blot) (Western blot analysis of TIM-3 in overexpressing HEK293 cells TIM-3 antibody at (A) 0.25, (B) 0.5, and (C) 1 μg/ml)

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TIM-3, Monoclonal Antibody (Cat# AAA11028)

Full Name

TIM-3 Antibody [2A6]

Gene Names

HAVCR2; TIM3; CD366; KIM-3; TIMD3; Tim-3; TIMD-3; HAVcr-2

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Protein A Purified

Pricing

$570/0.1 mg $1,080/2x0.1 mg $1,560/3x0.1 mg $2,015/4x0.1 mg $2,295/5x0.1 mg

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Application Data (Staining of mouse peripheral blood platelets with HAMSTER ANTI MOUSE CD61:RPE)

Application Data (Staining of mouse peripheral blood platelets with HAMSTER ANTI MOUSE CD61:FITC)

Application Data (Staining of mouse peripheral blood platelets with HAMSTER ANTI MOUSE CD61:FITC)

Application Data (Staining of mouse peripheral blood platelets with HAMSTER ANTI MOUSE CD61:BIOTIN)

Application Data (Staining of mouse peripheral blood platelets with HAMSTER ANTI MOUSE CD61:Alexa Fluor®647)

Application Data (Staining of mouse peripheral blood platelets with HAMSTER ANTI MOUSE CD61:Alexa Fluor®488)

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Integrin, beta3, Monoclonal Antibody (Cat# AAA26739)

Full Name

Integrin, beta3 (ITGB3, CD61, GP3A, GPIIIa, HPA-1, NAIT, Platelet Fibrinogen Receptor beta Subunit, Platelet Glycoprotein IIIa, Platelet Membrane Glycoprotein IIIa) (Biotin)

Reactivity

Mouse, Rat

Applications

Flow Cytometry, Immunoprecipitation

Purity

Purified by Protein G Affinity Chromatography

Pricing

$965/0.1 mL $1,860/2x0.1 mL $2,700/3x0.1 mL $3,495/4x0.1 mL $4,055/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using eNOS Rabbit mAb (AAA28545) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using eNOS Rabbit mAb (AAA28545) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using eNOS Rabbit mAb (AAA28545) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using eNOS Rabbit mAb (AAA28545) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using eNOS Rabbit mAb (AAA28545) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

WB (Western Blot) (Western blot analysis of various lysates using eNOS Rabbit mAb (AAA28545) at1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 180s.)

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eNOS, Monoclonal Antibody (Cat# AAA28545)

Full Name

eNOS Rabbit mAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Purity

Affinity purification

Pricing

$300/0.02 mL $500/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,780/4x0.1 mL $1,960/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of LOVO cells with Cellular Apoptosis Susceptibility antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining Cellular Apoptosis Susceptibility in SH-SY5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Cellular Apoptosis Susceptibility in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Cellular Apoptosis Susceptibility in LOVO cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Cellular Apoptosis Susceptibility antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Cellular Apoptosis Susceptibility antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Cellular Apoptosis Susceptibility on different lysates using anti-Cellular Apoptosis Susceptibility antibody at 1/500 dilution. Positive control: Lane 1: Mouse testis Lane 2: SiHa Lane 3: SK-BR-3 Lane 4: PC-3M)

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Cellular Apoptosis Susceptibility, Monoclonal Antibody (Cat# AAA30426)

Full Name

Cellular Apoptosis Susceptibility Antibody

Gene Names

CSE1L; CAS; CSE1; XPO2

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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ICC (Immunocytochemistry) (ICC staining NSE in 293 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining NSE in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-NSE antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-NSE antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of NSE on hybrid fish (crucian-carp) brain tissue lysates using anti-NSE antibody at 1/500 dilution.)

WB (Western Blot) (Western blot analysis of NSE on different lysates using anti-NSE antibody at 1/1, 000 dilution. Positive control: Lane 1: HepG2 Lane 2: Hela Lane 3: 293)

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NSE, Monoclonal Antibody (Cat# AAA30152)

Full Name

NSE Antibody

Gene Names

ENO2; NSE

Reactivity

Human, Mouse, Rat, Zebrafish

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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Application Data (Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 followed by horseradish peroxidase Goat anti Mouse IgG2a antibody as a detection reagent. Medium power)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD3:RPE)

Application Data (Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 followed by horseradish peroxidase Goat anti Mouse IgG2a antibody as a detection reagent. Low power)

Application Data (ELISA analysis of human CD3 expression using Rat anti Human CD3ε, clone CD3-12 as a capture reagent and biotinylated Mouse anti Human CD3, clone UCHT1 as a detection reagent with recombinant human CD3 as antigen to produce the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . A serum (green) sample is included undiluted)

Application Data (Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 followed by horseradish peroxidase Goat anti Mouse IgG2a antibody as a detection reagent. High power)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD3:Alexa Fluor 405)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD3)

Application Data (Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 followed by horseradish peroxidase Goat anti Mouse IgG2a antibody as a detection reagent. High power)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD3: RPE- Alexa Fluor 750)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD3: FITC)

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CD3, Monoclonal Antibody (Cat# AAA11877)

Full Name

MOUSE ANTI HUMAN CD3:FITC

Gene Names

CD3G; T3G; IMD17; CD3-GAMMA

Applications

Flow Cytometry

Pricing

$530/0.1 mg $1,005/2x0.1 mg $1,455/3x0.1 mg $1,885/4x0.1 mg $2,070/5x0.1 mg

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Application Data (Staining of mouse spleen cells with HAMSTER ANTI MOUSE CD29: Alexa Fluor® 647)

Application Data (Staining of mouse spleen cells with hamster anti mouse CD29: Alexa Fluor® 488)

Application Data (Staining of mouse peripheral blood lymphocytes with HAMSTER ANTI MOUSE CD29:FITC)

Application Data (Staining of mouse peripheral blood lymphocytes with HAMSTER ANTI MOUSE CD29: LOW ENDOTOXIN)

Application Data (Staining of mouse peripheral blood lymphocytes with HAMSTER ANTI MOUSE CD29)

Application Data (Staining of mouse peripheral blood lymphocytes with HAMSTER ANTI MOUSE CD29)

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Integrin, beta1, Monoclonal Antibody (Cat# AAA14730)

Full Name

Integrin, beta1 (CD29, Very Late Antigen, VLAb1, Platelet gplla, ITGB1, Fibrinogen Receptor beta Subunit, FNRB, Integrin VLA4 Subunit beta, MDF2, MSK12, Very Late Activation Protein beta Polypeptide, VLAB, VLAbeta) (PE)

Gene Names

ITGB1; CD29; FNRB; MDF2; VLAB; GPIIA; MSK12; VLA-BETA

Reactivity

Mouse, Rat

Applications

Flow Cytometry

Purity

Affinity Purified
Purified by Protein G affinity chromatography.

Pricing

$885/100 Tests $1,695/2x100 Tests $2,465/3x100 Tests $3,190/4x100 Tests $3,680/5x100 Tests

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WB (Western Blot) (RPS6KB1 monoclonal antibody (M03), clone 2C2. Western Blot analysis of RPS6KB1 expression in PC-12.)

WB (Western Blot) (RPS6KB1 monoclonal antibody (M03), clone 2C2. Western Blot analysis of RPS6KB1 expression in NIH/3T3.)

Application Data (Detection limit for recombinant GST tagged RPS6KB1 is approximately 0.1ng/ml as a capture antibody.)

WB (Western Blot) (RPS6KB1 monoclonal antibody (M03), clone 2C2 Western Blot analysis of RPS6KB1 expression in A-431 (Cat # L015V1).)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RPS6KB1 on A-431 cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RPS6KB1 on A-431 cell. [antibody concentration 10 ug/ml])

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RPS6KB1, Monoclonal Antibody (Cat# AAA26634)

Full Name

RPS6KB1 (Ribosomal Protein S6 Kinase, 70kD, Polypeptide 1, PS6K, S6K, S6K1, STK14A, p70(S6K)-alpha, p70-S6K, p70-alpha) (PE)

Gene Names

RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha

Applications

Immunofluorescence, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Staining of mouse peritoneal macrophages with RAT ANTI MOUSE F4/80 ANTIGEN:FITC)

Application Data (Staining of mouse peritoneal macrophages with RAT ANTI MOUSE F4/80 ANTIGEN: ALEXA 405)

Application Data (Staining of mouse J774 cells with RAT ANTI MOUSE F4/80 ANTIGEN: RPE)

Application Data (Staining of J774 cells with RAT ANTI MOUSE F4/80 ANTIGEN:Low Endotoxin)

Application Data (Staining of J774 cells with RAT ANTI MOUSE F4/80 ANTIGEN:Biotin)

Application Data (Frozen mouse spleen, using STAR72 as the secondary)

Application Data (Staining of J774 cells with RAT ANTI MOUSE F4/80 ANTIGEN:Biotin)

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EMR1, Monoclonal Antibody (Cat# AAA26737)

Full Name

EMR1 (EGF-like Module Containing Mucin-like Hormone Receptor-like 1, EMR1 Hormone Receptor, Cell Surface Glycoprotein EMR1, Cell Surface Glycoprotein F4/80, DD7A5-7, EGF-TM7, F4/80, Gpf480, Lymphocyte Antigen 71, Ly71, TM7LN3) (Biotin)

Reactivity

Mouse

Applications

Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Radioimmunoassay, Western Blot

Purity

Purified by protein G affinity chromatography from tissue culture supernatant.

Pricing

$1,170/0.1 mL $2,255/2x0.1 mL $3,280/3x0.1 mL $4,250/4x0.1 mL $4,985/5x0.1 mL

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Application Data

Application Data

IF (Immunofluorescence) (Immunofluorescence of HeLa cell using 253500 (40g/ml).)

IHC (Immunohistochemistry) (Immunoperoxidase of formalin-fixed paraffin-embedded human placenta using 253500 (3ug/ml.)

WB (Western Blot) (Western Blot analysis of YAP1 expression in Hela S3 NE using 253500.)

Application Data (Detection limit for recombinant GST tagged YAP1 is approximately 0.03ng/ml using 253500 as a capture antibody.)

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YAP1, Monoclonal Antibody (Cat# AAA26197)

Full Name

YAP1 (Yes-Associated Protein 1, 65kD, YAP, YAP2, YAP65, YKI) (Biotin)

Gene Names

YAP1; YAP; YKI; COB1; YAP2; YAP65

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Frozen mouse spleen stained with Rat anti Mouse CD169)

Application Data (Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD169 antibody clone 3D6.112, green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. High power)

Application Data (Mouse peritoneal macrophages stained with Rat anti Mouse CD169:FITC)

Application Data (Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 followed by Goat anti Rat IgG antibody as a detection reagent. Medium power)

Application Data (Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 followed by Goat anti Rat IgG antibody as a detection reagent. High power)

Application Data (Immunoperoxidase staining of Mouse lymph node cryosection stained with Rat anti Mouse CD169 antibody, clone 3D6.112 followed by Goat anti Rat IgG antibody as a detection reagent. Low power)

Application Data (Immunofluorescence staining of mouse lymph node cryosection with Rat anti Mouse CD169 antibody clone 3D6.112, green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained blue using DAPI. Low power)

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CD169, Monoclonal Antibody (Cat# AAA12204)

Full Name

RAT ANTI MOUSE CD169

Gene Names

Siglec1; Sn; Cd169; Siglec-1

Applications

Immunohistochemistry, Flow Cytometry, Immunofluorescence

Pricing

$660/0.2 mg $1,255/2x0.2 mg $1,825/3x0.2 mg $2,365/4x0.2 mg $2,650/5x0.2 mg

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Application Data (Published customer image: Increased accumulation of repair-associated macrophages surrounding collaterals in ischemic hind limbs is PAR2-dependent. (A) Stainings of CD206-positive macrophages (green) and SMA-positive vessels (red) in non-ischemic (control) and ischemic (ligated) hind limbs of WT, PAR1-/- and PAR2-/- mice are shown. Nuclei were visualized with DAPI (blue). Arrows indicate single macrophages in the non-ischemic adductor. Quantification of the average number of repair-associated macrophages per vessel is indicated on the right. (B) Correlation between the number of CD206-positive macrophages in the ischemic tissues and the expression of CD11b and (C) CD115 on monocytes. ** p)

Application Data (Published customer image: Western blot analysis to determine type of activation of adult primary microglial cells produced from wild-type and IL-1 KO mice stimulated with IL-4, IL-13 or IL-4/IL-13 with or without IL-1beta. (A) Representative western blotting data of primary microglial cells produced from wild-type (wild) and IL-1 KO mice and exposed for 24 hours to IL-4, IL-13 or IL-4 plus IL-13 (IL-4/IL-13) with or without IL-1beta. Each lane expected to CD206 blotting were applied 7 ug of reduced samples. Non-reduced samples (5 ug) were applied to detect CD206. Densitometric analysis of COX2 (B), Ym1 (C), Arg-1 (D) and CD206 (E) (n = 3 each group). (B) COX2 levels are increased by exposure of cells to IL-1beta and are not influenced by IL-4 or IL-13 alone. The COX2 level was slightly enhanced by IL-1beta and IL-4 co-treatment. (C) Ym1 levels are increased by exposure of cells to IL-4 and IL-4/Il-13 and are synergistically increased by co-treatment with IL-1beta. However, only a low level of Ym1 is seen upon exposure of cells to IL-13, and is significantly less than that seen in response to exposure of cells to IL-4. (D) Arg-1 shows similar levels in response to exposure to IL-4 and IL-4/IL-13; these are synergistically increased by co-treatment with IL-1beta. However, low levels of Arg-1 are seen for exposure of cells to IL-13. (G) CD206 was detected in response to exposure of cells to both IL-4 and IL-4/IL-13 with or without IL-1beta; however, CD206 levels in IL-13-exposed samples were lower than those seen with the other treatments. Data are expressed as mean +/- SD (n = 3). *: P < 0.05, **: P < 0.01, ***: P < 0.001 compared with the IL-4-treated group without IL-1beta for each genotype (one-way ANOVA followed by Dunnett post-hoc test). ANOVA, analysis of variance; arg-1, arginase 1; COX 2, cyclooxygenase 2.From: Sato A, Ohtaki H, Tsumuraya T, Song D, Ohara K, Asano M, Iwakura Y, Atsumi T, Shioda S. Interleukin-1 participates in the classical and alternative activation of microglia/macrophages after spinal cord injury. J Neuroinflammation. 2012 Apr 7;9:65.)

Application Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained in blue using DAPI. Low power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . High power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Low power)

Application Data (Staining of J774 cell line with Rat anti Mouse CD206 following permeabilisation with Leucoperm)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD206: Alexa Fluor 488)

Application Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained in blue using DAPI. High power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Medium power)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD206: Alexa Fluor 647)

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CD206, Monoclonal Antibody (Cat# AAA12122)

Full Name

RAT ANTI MOUSE CD206:FITC

Gene Names

Mrc1; MR; CD206; AW259686

Applications

Flow Cytometry

Pricing

$290/0.025 mg $530/2x0.025 mg $755/3x0.025 mg $970/5x0.025 mg $975/4x0.025 mg

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IHC (Immunohistchemistry) (Detection of SMARCB1/SNF5 by immunohistochemistry. Sample: FFPE section of ovarian carcinoma. Antibody: Rabbit anti-SMARCB1/SNF5 recombinant monoclonal antibody (AAA23883). Secondary: HRP-conjugated goat anti-rabbit IgG)

IHC (Immunohistochemistry) (Detection of SMARCB1/SNF5 by immunohistochemistry. Sample: FFPE section of mouse plasmacytoma. Antibody: Rabbit anti-SMARCB1/SNF5 recombinant monoclonal antibody (AAA23883). Secondary: HRP-conjugated goat anti-rabbit IgG)

ICC (Immunocytochemistry) (Detection of SMARCB1/SNF5 by immunocytochemistry. Sample: FFPE section of NCI-H226 cells. Antibody: Rabbit anti-SMARCB1/SNF5 recombinant monoclonal antibody (AAA23883). Secondary: HRP-conjugated goat anti-rabbit IgG)

ICC (Immunocytochemistry) (Detection of SMARCB1/SNF5 by immunocytochemistry. Sample: FFPE section of mouse 3T3-L1 cells. Antibody: Rabbit anti-SMARCB1/SNF5 recombinant monoclonal antibody (AAA23883). Secondary: HRP-conjugated goat anti-rabbit IgG)

FCM (Flow Cytometry) (Detection of human SMARCB1/SNF5 (shaded) in U2OS cells by flow cytometry. Antibody: Rabbit anti-SMARCB1/SNF5 recombinant monoclonal antibody (AAA23883) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

FCM (Flow Cytometry) (Detection of mouse SMARCB1/SNF5 (shaded) in EL4 cells by flow cytometry. Antibody: Rabbit anti-SMARCB1/SNF5 recombinant monoclonal antibody (AAA23883) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

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SMARCB1/SNF5, Monoclonal Recombinant Antibody (Cat# AAA23883)

Full Name

Rabbit anti-SMARCB1/SNF5 Recombinant Monoclonal Antibody [BLR306M]

Gene Names

SMARCB1; RDT; INI1; SNF5; Snr1; BAF47; MRD15; RTPS1; Sfh1p; hSNFS; SNF5L1; SWNTS1; PPP1R144

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunocytochemistry, Flow Cytometry

Purity

Purified

Pricing

$295/0.01 mL $680/0.1 mL $980/5x0.01 mL $1,300/2x0.1 mL $1,890/3x0.1 mL $2,440/4x0.1 mL $2,755/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of K562 cells with UBA1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining UBA1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining UBA1 in SKOV-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining UBA1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-UBA1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse ovary tissue using anti-UBA1 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of UBA1 on mouse liver lysates using anti-UBA1 antibody at 1/5, 000 dilution.)

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Ubiquitin-Like Modifier-Activating Enzyme 1, Monoclonal Antibody (Cat# AAA30213)

Full Name

Ubiquitin-Like Modifier-Activating Enzyme 1 Antibody

Gene Names

UBA1; A1S9; A1ST; GXP1; UBE1; A1S9T; AMCX1; POC20; SMAX2; UBA1A; UBE1X; CFAP124

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged PTBP1 is ~0.1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of PTBP1 transfected lysate using PTBP1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PTBP1 rabbit polyclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PTBP1 on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PTBP1 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of PTBP1 expression in transfected 293T cell line by PTBP1 monoclonal antibody, Lane 1: PTBP1 transfected lysate (59.6kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (PTBP1 monoclonal antibody, Western Blot analysis of PTBP1 expression in A-431.)

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PTBP1, Monoclonal Antibody (Cat# AAA25219)

Full Name

PTBP1 (Polypyrimidine Tract-binding Protein 1, PTB, Heterogeneous Nuclear Ribonucleoprotein I, hnRNP I, 57kD RNA-binding Protein PPTB-1, MGC10830, MGC8461) (FITC)

Gene Names

PTBP1; PTB; PTB2; PTB3; PTB4; pPTB; HNRPI; PTB-1; PTB-T; HNRNPI; HNRNP-I

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using SUN2 Rabbit mAb (AAA28533) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using SUN2 Rabbit mAb (AAA28533) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma tissue using SUN2 Rabbit mAb (AAA28533) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using SUN2 Rabbit mAb (AAA28533) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using SUN2 Rabbit mAb (AAA28533) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

WB (Western Blot) (Western blot analysis of various lysates using SUN2 Rabbit mAb (AAA28533) at 1:2000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 60s.)

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SUN2, Monoclonal Antibody (Cat# AAA28533)

Full Name

SUN2 Rabbit mAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Purity

Affinity purification

Pricing

$285/0.02 mL $465/0.1 mL $885/2x0.1 mL $1,275/3x0.1 mL $1,650/4x0.1 mL $1,765/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged CDH11 is 3 ng/ml as a capture antibody.)

Application Data (Detection limit for recombinant GST tagged CDH11 is 1 ng/ml as a capture antibody.)

WB (Western Blot) (CDH11 monoclonal antibody (M05), clone 3C8. Western Blot analysis of CDH11 expression in human kidney.)

WB (Western Blot) (CDH11 monoclonal antibody (M05), clone 3C8. Western Blot analysis of CDH11 expression in MCF-7.)

Application Data (PC3-MM2 cells were stained with CDH11-FITC labeled monoclonal antibody (Green). The cell nucleus were counterstained with DAPI (Blue).)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CDH11 on PC3-MM2 cell. [antibody concentration 10 ug/ml])

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CDH11, Monoclonal Antibody (Cat# AAA26278)

Full Name

CDH11 (Cadherin 11, Type 2, OB-Cadherin (Osteoblast), CAD11, CDHOB, OB, OSF-4) (Biotin)

Gene Names

CDH11; OB; CAD11; CDHOB; OSF-4

Applications

Immunofluorescence, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged YY1 is approximately 0.03ng/ml as a capture antibody.)

WB (Western Blot) (YY1 monoclonal antibody (M04), clone 2C5 Western Blot analysis of YY1 expression in Hela S3 NE (Cat # L013V3).)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to YY1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to YY1 on HeLa cell. [antibody concentration 10 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to YY1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 7 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to YY1 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 7 ug/ml])

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YY1, Monoclonal Antibody (Cat# AAA26442)

Full Name

YY1 (YY1 Transcription Factor, DELTA, INO80S, NF-E1, UCRBP, YIN-YANG-1) (HRP)

Gene Names

YY1; DELTA; NF-E1; UCRBP; GADEVS; INO80S; YIN-YANG-1

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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ICC (Immunocytochemistry) (Confocal imaging of paraffin-embedded human kidney using CD31/PECAM1 Rabbit mAb (AAA28467, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.)

ICC (Immunocytochemistry) (Confocal imaging of paraffin-embedded human placenta using CD31/PECAM1 Rabbit mAb (AAA28467, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x.Perform high pressure antigen retrieval with 0.01M citrate buffer (pH 6.0) prior to IF staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human thyroid using CD31/PECAM1 Rabbit mAb (AAA28467) at dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human liver using CD31/PECAM1 Rabbit mAb (AAA28467) at dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human kidney using CD31/PECAM1 Rabbit mAb (AAA28467) at dilution of 1:600 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

WB (Western Blot) (Western blot analysis of various lysates, using CD31/PECAM1 (AAA28467) at 1:12500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 180s.)

WB (Western Blot) (Western blot analysis of various lysates, using CD31/PECAM1 (AAA28467) at 1:12500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 180s.)

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CD31/PECAM1, Monoclonal Antibody (Cat# AAA28467)

Full Name

CD31/PECAM1 Rabbit mAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Affinity purification

Pricing

$300/0.02 mL $500/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,780/4x0.1 mL $1,960/5x0.1 mL

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WB (Western Blot) (Western blot analysis of Caspase-9 on different lysates using anti-Caspase-9 antibody at 1/1, 000 dilution. Positive control: Lane 1: Jurkat Lane 2: Hela Lane 3: C2C12)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-Caspase-9 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Caspase-9 antibody. Counter stained with hematoxylin.)

ICC (Immunocytochemistry) (ICC staining Caspase-9 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Caspase-9 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Caspase-9 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

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Caspase-9, Monoclonal Antibody (Cat# AAA30007)

Full Name

Caspase-9 Antibody

Gene Names

CASP9; MCH6; APAF3; APAF-3; PPP1R56; ICE-LAP6

Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of USF2 expression in transfected 293T cell line by USF2 monoclonal antibody,. Lane 1: USF2 transfected lysate (37kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (USF2 monoclonal antibody. Western Blot analysis of USF2 expression in different cell lines.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to USF2 on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to USF2 on formalin-fixed paraffin-embedded human cerebral cortex. [antibody concentration 1ug/ml])

Application Data (Detection limit for recombinant GST tagged USF2 is ~0.3ng/ml as a capture antibody.)

WB (Western Blot) (USF2 monoclonal antibody. Western Blot analysis of USF2 expression in PC-12.)

WB (Western Blot) (USF2 monoclonal antibody Western Blot analysis of USF2 expression in HeLa.)

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USF2, Monoclonal Antibody (Cat# AAA24698)

Full Name

USF2 (Upstream Stimulatory Factor 2, Class B Basic Helix-loop-helix Protein 12, bHLHb12, FOS-interacting Protein, FIP, Major Late Transcription Factor 2, Upstream Transcription Factor 2) APC

Gene Names

USF2; FIP; bHLHb12

Reactivity

Human, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Phospho-POLR2A (S2) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining Phospho-POLR2A (S2) in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Phospho-POLR2A (S2) in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Phospho-POLR2A (S2) in Hela cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Phospho-POLR2A (S2) antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Phospho-POLR2A (S2) antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Phospho-POLR2A (S2) antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Phospho-POLR2A (S2) on differnt cells lysates using anti- Phospho-POLR2A (S2) antibody at 1/500 dilution. Positive control�� Line 1: Hela Line 2: MCF-7)

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POLR2A, Monoclonal Antibody (Cat# AAA29786)

Full Name

POLR2A (Phospho-S2) Antibody

Gene Names

POLR2A; RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,015/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using MUC1 Rabbit mAb (AAA28481) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using MUC1 Rabbit mAb (AAA28481) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using MUC1 Rabbit mAb (AAA28481) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using MUC1 Rabbit mAb (AAA28481) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)

IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using MUC1 Rabbit mAb (AAA28481) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using MUC1 Rabbit mAb (AAA28481) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human kidney tissue using MUC1 Rabbit mAb (AAA28481) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)

IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon tissue using MUC1 Rabbit mAb (AAA28481) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using MUC1 Rabbit mAb (AAA28481) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using MUC1 Rabbit mAb (AAA28481) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using MUC1 Rabbit mAb (AAA28481) at a dilution of 1:400 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer(pH 9.0) prior to IHC staining.)

WB (Western Blot) (Western blot analysis of lysates from Rat lung, using MUC1 Rabbit mAb (AAA28481) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 1min.)

WB (Western Blot) (Western blot analysis of lysates from Rat lung, using MUC1 Rabbit mAb (AAA28481) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 5s.)

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MUC1, Monoclonal Antibody (Cat# AAA28481)

Full Name

MUC1 Rabbit mAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry

Purity

Affinity purification

Pricing

$300/0.02 mL $500/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,780/4x0.1 mL $1,960/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of THP-1 cells with Gelsolin antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining Gelsolin in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Gelsolin in NIH-3T3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Gelsolin antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse lung tissue using anti-Gelsolin antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Gelsolin antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Gelsolin on different lysates using anti-Gelsolin antibody at 1/500 dilution. Positive control: Lane 1: Mouse thymus Lane 2: Mouse lung Lane 3: THP-1 Lane 4: HepG2)

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Gelsolin, Monoclonal Antibody (Cat# AAA30452)

Full Name

Gelsolin Antibody

Gene Names

GSN; ADF; AGEL

Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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WB (Western Blot) (Detection of mouse Galectin-9 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, Ba/F3, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Galectin-9 recombinant monoclonal antibody [BL-1675A-4A5] (AAA23862 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes.)

WB (Western Blot) (Detection of human Galectin-9 by western blot. Samples: Whole cell lysate (10 ug) from Jurkat, THP-1, and MOLT-4 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Galectin-9 recombinant monoclonal antibody (AAA23862 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 75 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)

IHC (Immunohistochemistry) (Detection of mouse Galectin-9/Gal-9 by immunohistochemistry. Sample: FFPE section of mouse spleen. Antibody: Rabbit anti-Galectin-9/Gal-9 recombinant monoclonal antibody (AAA23862-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

IHC (Immunohistochemistry) (Detection of human Galectin-9/Gal-9 by immunohistochemistry. Sample: FFPE section of breast carcinoma. Antibody: Rabbit anti-Galectin-9/Gal-9 recombinant monoclonal antibody (AAA23862-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

IHC (Immunohistchemistry) (Detection of human Galectin-9/Gal-9 by immunohistochemistry. Sample: FFPE section of bladder carcinoma. Antibody: Rabbit anti-Galectin-9/Gal-9 recombinant monoclonal antibody (AAA23862 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

ICC (Immunocytochemistry) (Detection of human Galectin-9/Gal-9 by immunocytochemistry. Sample: FFPE section of HL-60 cells. Antibody: Rabbit anti-Galectin-9/Gal-9 recombinant monoclonal antibody (AAA23862-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

ICC (Immunocytochemistry) (Detection of human Galectin-9/Gal-9 by immunocytochemistry. Sample: FFPE section of jurkat cells. Antibody: Rabbit anti-Galectin-9/Gal-9 recombinant monoclonal antibody (AAA23862-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

ICC (Immunocytochemistry) (Detection of mouse Galectin-9/Gal-9 by immunocytochemistry. Sample: FFPE section of BW5147.3 cells. Antibody: Rabbit anti-Galectin-9/Gal-9 recombinant monoclonal antibody (AAA23862-1). Secondary: HRP-conjugated goat anti-rabbit IgG .)

FCM (Flow Cytometry) (Detection of human Galectin-9/Gal-9 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-Galectin-9 recombinant monoclonal antibody (AAA23862) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

FCM (Flow Cytometry) (Detection of mouse Galectin-9/Gal-9 (shaded) in NIH3T3 cells by flow cytometry. Antibody: Rabbit anti-Galectin-9 recombinant monoclonal antibody (AAA23862) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

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Galectin-9/Gal-9, Monoclonal Recombinant Antibody (Cat# AAA23862)

Full Name

Rabbit anti-Galectin-9/Gal-9 Recombinant Monoclonal Antibody [BLR193J]

Gene Names

LGALS9; HUAT; LGALS9A

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry

Purity

Purified

Pricing

$295/0.01 mL $680/0.1 mL $980/5x0.01 mL $1,300/2x0.1 mL $1,890/3x0.1 mL $2,440/4x0.1 mL $2,755/5x0.1 mL

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WB (Western Blot) (DNAJC10 monoclonal antibody. Western Blot analysis of DNAJC10 expression in HeLa.)

WB (Western Blot) (DNAJC10 monoclonal antibody. Western Blot analysis of DNAJC10 expression in Raw 264.7.)

Application Data (Detection limit for recombinant GST tagged DNAJC10 is ~10ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to DNAJC10 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 3ug/ml].)

WB (Western Blot) (DNAJC10 monoclonal antibody. Western Blot analysis of DNAJC10 expression in NIH/3T3.)

WB (Western Blot) (Western Blot detection against Immunogen (37.77kD).)

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DNAJC10, Monoclonal Antibody (Cat# AAA25375)

Full Name

DNAJC10 (DnaJ Homolog Subfamily C Member 10, ER-resident Protein ERdj5, Macrothioredoxin, MTHr, ERDJ5, UNQ495/PRO1012, DKFZp434J1813, MGC104194) (HRP)

Gene Names

DNAJC10; JPDI; MTHr; ERdj5; PDIA19

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (FOXA1 monoclonal antibody (M03), clone 4F6 Western Blot analysis of FOXA1 expression in A-431 (Cat # L015V1).)

WB (Western Blot) (FOXA1 monoclonal antibody (M03), clone 4F6 Western Blot analysis of FOXA1 expression in HepG2 (Cat # L019V1).)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA1 on A-431 cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA1 on A-431 cell. [antibody concentration 10 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FOXA1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to FOXA1 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3 ug/ml])

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FOXA1, Monoclonal Antibody (Cat# AAA26489)

Full Name

FOXA1 (Forkhead Box A1, HNF3A, MGC33105, TCF3A) (HRP)

Gene Names

FOXA1; HNF3A; TCF3A

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Staining of mouse spleen cells with HAMSTER ANTI MOUSE CD29: Alexa Fluor® 647)

Application Data (Staining of mouse spleen cells with hamster anti mouse CD29: Alexa Fluor® 488)

Application Data (Staining of mouse peripheral blood lymphocytes with HAMSTER ANTI MOUSE CD29:FITC)

Application Data (Staining of mouse peripheral blood lymphocytes with HAMSTER ANTI MOUSE CD29: LOW ENDOTOXIN)

Application Data (Staining of mouse peripheral blood lymphocytes with HAMSTER ANTI MOUSE CD29)

Application Data (Staining of mouse peripheral blood lymphocytes with HAMSTER ANTI MOUSE CD29)

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Integrin, beta1, Monoclonal Antibody (Cat# AAA26763)

Full Name

Integrin, beta1 (CD29, Very Late Antigen, VLAb1, Platelet gplla, ITGB1, Fibrinogen Receptor beta Subunit, FNRB, Integrin VLA4 Subunit beta, MDF2, MSK12, Very Late Activation Protein beta Polypeptide, VLAB, VLAbeta) (HRP)

Reactivity

Mouse, Rat

Applications

Flow Cytometry, Immunoprecipitation

Purity

Purified by Protein G Affinity Chromatography

Pricing

$1,010/0.1 mL $1,950/2x0.1 mL $2,835/3x0.1 mL $3,675/4x0.1 mL $4,260/5x0.1 mL

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WB (Western Blot) (Western blot analysis of ARID3A over-expressed 293 cell line, cotransfected with ARID3A Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ARID3A monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged ARID3A is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ARID3A on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ARID3A on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml].)

WB (Western Blot) (ARID3A monoclonal antibody, Western Blot analysis of ARID3A expression in K-562.)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

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ARID3A, Monoclonal Antibody (Cat# AAA25615)

Full Name

ARID3A (AT-rich Interactive Domain-containing Protein 3A, ARID Domain-containing Protein 3A, B Cell Regulator of IgH Transcription, Bright, Dead Ringer-like Protein 1, E2F-binding Protein 1, DRIL1, DRIL3, DRX, E2FBP1) (PE)

Gene Names

ARID3A; DRIL1; DRIL3; BRIGHT; E2FBP1

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Staining of rat splenocytes with Mouse anti Rat MHC class II RT1B)

Application Data (Staining of rat spleen cells with Mouse anti Rat RT1B)

Application Data (Staining of rat spleen cells with Mouse anti Rat RT1B: FITC)

Application Data (Staining of rat spleen cells with Mouse anti Rat RT1B: RPE)

Application Data (Staining of rat spleen cells with Mouse anti Rat RT1B: FITC)

Application Data (Rat Spleen Tissue lysate probed with Mouse anti Rat MHC Class II RT1B)

Application Data (Staining of rat spleen cells with Mouse anti Rat RTIB: Alexa Fluor 647)

Application Data (Staining of rat spleen cells with Mouse anti Rat RT1B: Alexa Fluor 488)

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MHC CLASS II RT1B, Monoclonal Antibody (Cat# AAA12157)

Full Name

MOUSE ANTI RAT MHC CLASS II RT1B:FITC

Applications

Flow Cytometry

Pricing

$555/0.1 mg $1,045/2x0.1 mg $1,510/3x0.1 mg $1,955/4x0.1 mg $2,165/5x0.1 mg

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Application Data (Staining of mouse peripheral blood granulocytes with Rat anti Mouse CD13)

Application Data (Staining of J774 cells with Rat anti Mouse CD13:Alexa Fluor 488 (AAA12144A488))

Application Data (Immunoperoxidase staining of a mouse tail cryosection with Rat anti Mouse CD13 antibody, clone ER-BMDM1 followed by horseradish peroxidase conjugated Goat anti Rat IgG Low power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD13 antibody, clone ER-BMDM1 followed by horseradish peroxidase conjugated Goat anti Rat IgG Medium power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD13 antibody, clone ER-BMDM1 followed by horseradish peroxidase conjugated Goat anti Rat IgG High power)

Application Data (Immunoperoxidase staining of a mouse tail cryosection with Rat anti Mouse CD13 antibody, clone ER-BMDM1 followed by horseradish peroxidase conjugated Goat anti Rat IgG High power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD13 antibody, clone ER-BMDM1 followed by horseradish peroxidase conjugated Goat anti Rat IgG Low power)

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CD13, Monoclonal Antibody (Cat# AAA12144)

Full Name

RAT ANTI MOUSE CD13

Gene Names

Anpep; Apn; AP-M; AP-N; Cd13; P150

Applications

Immunohistochemistry, Flow Cytometry

Purity

Affinity chromatography on Protein G from tissue culture supernatant

Pricing

$660/0.25 mg $1,255/2x0.25 mg $1,825/3x0.25 mg $2,365/4x0.25 mg $2,650/5x0.25 mg

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Application Data (Staining of CD209 transfected K562 cells with MOUSE ANTI HUMAN CD209: FITC)

Application Data (Staining of CD209 transfected K562 cells with MOUSE ANTI HUMAN CD209: BIOTIN)

Application Data (Staining of CD209 transfected K562 cells with MOUSE ANTI HUMAN CD209: Alexa 647)

Application Data (Staining of CD209 transfected K562 cells with MOUSE ANTI HUMAN CD209: Alexa 488)

Application Data (Staining of CD209 transfected K562 cells with MOUSE ANTI HUMAN CD209)

Application Data (Staining of CD209 transfected K562 cells with MOUSE ANTI HUMAN CD209)

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DC-SIGN, Monoclonal Antibody (Cat# AAA26799)

Full Name

DC-SIGN (Dendritic Cell-specific ICAM3 grabbing Non-integrin, C Type Lectin Domain Family 4 Member L, CLEC4L, CD209, CD209 Antigen, CDSIGN, Dendritic Cell-specific ICAM-3-grabbing Non-integrin 1, DC SIGN1, DC-SIGN1, HIV GP120 Binding Protein, MGC129965) (

Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence

Purity

Purified by protein G affinity chromatography from tissue culture supernatant.

Pricing

$1,245/0.1 mL $2,410/2x0.1 mL $3,500/3x0.1 mL $4,535/4x0.1 mL $5,325/5x0.1 mL

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WB (Western Blot) (Western blot analysis of ENO3 over-expressed 293 cell line, cotransfected with ENO3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ENO3 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged ENO3 is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to ENO3 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ENO3 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to ENO3 on formalin-fixed paraffin-embedded human stomach. [antibody concentration 1.5ug/ml].)

WB (Western Blot) (Western Blot analysis of ENO3 expression in transfected 293T cell line by ENO3 monoclonal antibody.Lane 1: ENO3 transfected lysate (46.9kD).Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (31.24kD).)

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ENO3, Monoclonal Antibody (Cat# AAA24080)

Full Name

ENO3 (Enolase 3, 2-phospho-D-glycerate Hydro-lyase, beta-Enolase, Muscle-specific Enolase, MSE, Skeletal Muscle Enolase)

Gene Names

ENO3; MSE; GSD13

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purified
Purified by Protein A affinity chromatography.

Pricing

$820/0.1 mg $1,565/2x0.1 mg $2,270/3x0.1 mg $2,940/4x0.1 mg $3,380/5x0.1 mg

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WB (Western Blot) (Detection of mouse NF-H by western blot. Samples: Whole cell lysate (50 ug) from CT26, NIH 3T3, ND7/23, TCMK-1 (10 ug), and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-NF-H recombinant monoclonal antibody (AAA23859 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody .)

WB (Western Blot) (Detection of human NF-H by western blot. Samples: Whole cell lysate (50 ug) from Jurkat, OVCAR-8, HEK293T, SK-N-BE(2), and HeLa cells prepared using NETN lysis buffer. Antibody: Rabbit anti-NF-H recombinant monoclonal antibody (AAA23859 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-COPB2 antibody .)

IP (Immunoprecipitation) (Detection of human NF-H by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from OVCAR-8 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-NF-H recombinant monoclonal antibody (AAA23859 lot 1) used for IP at 20 ul/mg lysate. NF-H was also immunoprecipitated by a second antibody against a different epitope (BL-2008A-1E7). For blotting immunoprecipitated NF-H, AAA23859 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 75 seconds.)

IHC (Immunohistochemistry) (Detection of mouse NF-H in mouse cerebellum by IHC. Antibody: Rabbit anti-NF-H recombinant monoclonal antibody (AAA23859 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB)

IHC (Immunohistochemistry) (Detection of human NF-H in brain by IHC. Antibody: Rabbit anti-NF-H recombinant monoclonal antibody (AAA23859 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB)

ICC (Immunocytochemistry) (Detection of human NF-H in SK-N-BE(2) cells by ICC. Antibody: Rabbit anti-NF-H recombinant monoclonal antibody (AAA23859 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB)

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NF-H, Monoclonal Recombinant Antibody (Cat# AAA23859)

Full Name

Rabbit anti-NF-H Recombinant Monoclonal Antibody [BLR189J]

Gene Names

NEFH; NFH

Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry

Purity

Purified

Pricing

$295/0.01 mL $680/0.1 mL $980/5x0.01 mL $1,300/2x0.1 mL $1,890/3x0.1 mL $2,440/4x0.1 mL $2,755/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of AFP expression in transfected 293T cell line by AFP monoclonal antibody. Lane 1: AFP transfected lysate (69kD). Lane 2: Non-transfected lysate.)

Application Data (Detection limit for recombinant GST tagged AFP is ~0.03ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of AFP transfected lysate using AFP monoclonal antibody and Protein A Magnetic Bead and immunoblotted with AFP rabbit polyclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to AFP on HepG2 cell. [antibody concentration 30ug/ml].)

WB (Western Blot) (AFP monoclonal antibody Western Blot analysis of AFP expression in HepG2.)

WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)

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Fetoprotein, Alpha, Monoclonal Antibody (Cat# AAA24127)

Full Name

Fetoprotein, Alpha (AFP, Alpha1-fetoprotein, Alpha-fetoglobulin, Alpha-fetoprotein precursor, FETA, HPAFP) (AP)

Gene Names

AFP; AFPD; FETA; HPAFP

Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of DHFR expression in HeLa.)

IF (Immunofluorescence) (Immunofluorescence on HeLa cells using 125814 (10ug/ml).)

Application Data (Detection limit for 125814 is ~0.1ng/ml as a capture antibody.)

WB (Western Blot) (Western Blot analysis of DHFR expression in transfected 293T cell line using 125814. Lane 1: DHFR transfected lysate (21.5kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot analysis of DHFR expression in PC-12 using 125814.)

WB (Western Blot) (Western Blot detection against immunogen (36.74kD).)

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DHFR, Monoclonal Antibody (Cat# AAA25372)

Full Name

DHFR (Dihydrofolate reductase) (HRP)

Gene Names

DHFR; DYR; DHFRP1

Reactivity

Human, Rat

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of Human-stomach-cancer tissue. 1.Ubiquitin Mouse Monoclonal Antibody(5F1)(red) was diluted at 1:200(4 degree C.overnight). 2. Cy3 labled Secondary antibody was diluted at 1:300(room temperature. 50min).3. Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1.Ubiquitin Mouse Monoclonal Antibody(5F1) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human-Tonsil tissue. 1.Ubiquitin Mouse Monoclonal Antibody(5F1) was diluted at 1:200(4 degree C.overnight). 2. Sodium citrate pH 6.0 was used for antibody retrieval(>98 degree C.20min). 3.Secondary antibody was diluted at 1:200(room temperature. 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Stomach Carcinoma Tissue using Ubiquitin Mouse mAb diluted at 1:200.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human Breast Carcinoma Tissue using Ubiquitin Mouse mAb diluted at 1:200.)

WB (Western Blot) (Western blot analysis of 1) Hela Cell Lysate. 2) 3T3 Cell Lysate. 3) Rat Brain Tissue Lysate using Ubiquitin Mouse mAb diluted at 1:1000.)

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Ubiquitin, Monoclonal Antibody (Cat# AAA30830)

Full Name

Ubiquitin Mouse Monoclonal Antibody (5F1)

Gene Names

RPS27A; UBC; S27A; CEP80; UBA80; HEL112; UBCEP1; UBCEP80

Reactivity

Human, Rat, Mouse, Pig

Applications

Western Blot, Immunofluorescence, Immunocytochemistry, Immunohistochemistry

Purity

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Pricing

$300/0.04 mL $390/0.1 mL $555/0.2 mL $1,045/2x0.2 mL $1,510/3x0.2 mL $1,955/4x0.2 mL $2,150/5x0.2 mL

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Application Data

Application Data

Application Data

Application Data

Application Data

SDS-PAGE (Whole cell lysates were separated by 6% SDS-PAGE, and the membrane was blotted with anti-Ki-67(ABT001)antibody. The HRP-conjugated Goat anti-M)

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Ki-67, Monoclonal Antibody (Cat# AAA30902)

Full Name

Ki-67 (ABT001) Antibody

Gene Names

MKI67; KIA

Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Immunofluorescence

Purity

Protein G

Pricing

$240/0.02 mL $315/0.05 mL $375/0.1 mL $600/0.2 mL $1,140/2x0.2 mL $1,650/3x0.2 mL $2,135/4x0.2 mL $2,350/5x0.2 mL

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WB (Western Blot) (PPIE monoclonal antibody Western Blot analysis of PPIE expression in PC-12.)

WB (Western Blot) (PPIE monoclonal antibody Western Blot analysis of PPIE expression in Hela NE.)

Application Data (Detection limit for recombinant GST tagged PPIE is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PPIE on HeLa cell. [antibody concentration 10ug/ml])

WB (Western Blot) (Western Blot analysis of PPIE expression in transfected 293T cell line by PPIE monoclonal antibody. Lane 1: PPIE transfected lysate (33.4kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (PPIE monoclonal antibody Western Blot analysis of PPIE expression in NIH/3T3.)

WB (Western Blot) (PPIE monoclonal antibody Western Blot analysis of PPIE expression in Raw 264.7.)

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Cyclophilin E, Monoclonal Antibody (Cat# AAA25659)

Full Name

Cyclophilin E (Cyclophilin 33, CYP33, CYP-33, MGC3736, MGC111222, Peptidyl-Prolyl Cis-Trans Isomerase E, PPIase E, PPIE, Rotamase E, Rotamase-E) (PE)

Gene Names

PPIE; CYP33; CYP-33

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Published customer image:Rat anti Mouse CD301 antibody, clone ER-MP23 used to identify M2 macrophages in murine adipose tissue by immunofluorescence.Image caption:Expression of M2 markers CD206 and CD301 by ATMs of induced CLS and interstitial macrophages. M2 ATMs outside of CLS are highlighted by arrows. Scale bars?=?50?um.From: Lindhorst A, Raulien N, Wieghofer P, Eilers J, Rossi FMV, Bechmann I, Gericke M.Adipocyte death triggers a pro-inflammatory response and induces metabolic activation of resident macrophages.)

Application Data (Published customer image:Insert meta-description and applicationsImage caption:Insert image caption)

Application Data (Published customer image:Rat anti Mouse CD301 antibody, clone ER-MP23 used for the detection of CD301 positive cells in murine atherosclerotic lesions by immunofluorescence.Image caption:Characterization of the M1 and M2 macrophage subsets in the vulnerable atherosclerotic lesion. (a) Double confocal immunofluorescence of atherosclerotic lesions showing CD68 (macrophage) and CD86 (M1 marker) on top or CD86 and CD301 (M2 marker) in the bottom. Arrows point double positive cells for CD68 and CD86 or CD301. (b) Quantification of lesional CD68-CD86 (top) or CD68-CD301 (bottom) area in the models (n=7-8; with 2-tailed t test). Cast HFD and LCCA LRA CD. LCCA: left common carotid artery; LRA: left renal artery; CD: chow diet; HFD: high fat diet.)

Application Data (Immunoperoxidase staining of a mouse lymph node with Rat anti Mouse CD301 antibody, clone ER-MP23 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . High power.)

Application Data (Immunoperoxidase staining of a mouse lymph node with Rat anti Mouse CD301 antibody, clone ER-MP23 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Medium power.)

Application Data (Immunoperoxidase staining of a mouse lymph node with Rat anti Mouse CD301 antibody, clone ER-MP23 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Low power.)

Application Data (Figure A. PE conjugated Rat anti Mouse CD11b and Alexa Fluor 700 conjugated Rat IgG2a isotype control . Figure B. PE conjugated Rat anti Mouse CD11b and Alexa Fluor 700 conjugated Rat anti Mouse CD301 . All experiments performed on mouse peritoneal cells gated on live single monocytes, in the presence of 10% mouse serum. Data acquired on the ZE5 Cell Analyzer.)

Application Data (Published customer image:Rat anti Mouse CD301 antibody, clone ER-MP23 used for the detection of CD301 positive cells in murine atherosclerotic lesions by immunofluorescence.Image caption:Characterization of the M1 and M2 macrophage subsets in the vulnerable atherosclerotic lesion. (a) Double confocal immunofluorescence of atherosclerotic lesions showing CD68 (macrophage) and CD86 (M1 marker) on top or CD86 and CD301 (M2 marker) in the bottom. Arrows point double positive cells for CD68 and CD86 or CD301. (b) Quantification of lesional CD68-CD86 (top) or CD68-CD301 (bottom) area in the models (n=7-8; with 2-tailed t test). Cast HFD and LCCA LRA CD. LCCA: left common carotid artery; LRA: left renal artery; CD: chow diet; HFD: high fat diet.)

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CD301, Monoclonal Antibody (Cat# AAA12294)

Full Name

RAT ANTI MOUSE CD301:RPE

Reactivity

Mouse

Applications

Flow Cytometry

Purity

Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Pricing

$625/100 Tests $1,190/2x100 Tests $1,720/3x100 Tests $2,225/4x100 Tests $2,490/5x100 Tests

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WB (Western Blot) (TRIB2 monoclonal antibody Western Blot analysis of TRIB2 expression in Jurkat.)

WB (Western Blot) (Western blot analysis of TRIB2 over-expressed 293 cell line, cotransfected with TRIB2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TRIB2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged TRIB2 is ~0.03ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TRIB2 on formalin-fixed paraffin-embedded human colon. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of TRIB2 expression in transfected 293T cell line by TRIB2 monoclonal antibody. Lane 1: TRIB2 transfected lysate (38.8kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (36.01kD).)

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TRIB2, Monoclonal Antibody (Cat# AAA24392)

Full Name

TRIB2 (Tribbles Homolog 2, TRB2, TRB-2, C5FW, GS3955) (AP)

Gene Names

TRIB2; C5FW; TRB2; GS3955

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IHC (Immunohistchemistry) (Detection of human SMARCA2/BRM by immunohistochemistry. Sample: FFPE section of ovarian carcinoma. Antibody: Rabbit anti-SMARCA/BRM recombinant monoclonal antibody (AAA23882). Secondary: HRP-conjugated goat anti-rabbit IgG .)

IHC (Immunohistochemistry) (Detection of mouse SMARCA2/BRM by immunohistochemistry. Sample: FFPE section of kidney. Antibody: Rabbit anti-SMARCA/BRM recombinant monoclonal antibody (AAA23882). Secondary: HRP-conjugated goat anti-rabbit IgG .)

ICC (Immunocytochemistry) (Detection of mouse SMARCA2/BRM by immunocytochemistry. Sample: FFPE section of J774A.1 cells. Antibody: Rabbit anti-SMARCA/BRM recombinant monoclonal antibody (AAA23882). Secondary: HRP-conjugated goat anti-rabbit IgG .)

ICC (Immunocytochemistry) (Detection of human SMARCA2/BRM by immunocytochemistry. Sample: FFPE section of VLN3G2 cells. Antibody: Rabbit anti-SMARCA/BRM recombinant monoclonal antibody (AAA23882). Secondary: HRP-conjugated goat anti-rabbit IgG .)

FCM (Flow Cytometry) (Detection of mouse SMARCA2/BRM (shaded) in NIH3T3 cells by flow cytometry. Antibody: Rabbit anti-SMARCA2/BRM recombinant monoclonal antibody (AAA23882) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

FCM (Flow Cytometry) (Detection of human SMARCA2/BRM (shaded) in HeLa cells by flow cytometry. Antibody: Rabbit anti-SMARCA2/BRM recombinant monoclonal antibody (AAA23882) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

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SMARCA2/BRM, Monoclonal Recombinant Antibody (Cat# AAA23882)

Full Name

Rabbit anti-SMARCA2/BRM Recombinant Monoclonal Antibody [BLR302M]

Gene Names

SMARCA2; BRM; SNF2; SWI2; hBRM; NCBRS; Sth1p; BAF190; SNF2L2; SNF2LA; hSNF2a

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunocytochemistry, Flow Cytometry

Purity

Purified

Pricing

$295/0.01 mL $680/0.1 mL $980/5x0.01 mL $1,300/2x0.1 mL $1,890/3x0.1 mL $2,440/4x0.1 mL $2,755/5x0.1 mL

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WB (Western Blot) (F11R monoclonal antibody, Western Blot analysis of F11R expression in HepG2.)

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between PRKCZ and F11R. HeLa cells were stained with PRKCZ rabbit purified polyclonal 1:1200 and F11R mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Application Data (Detection limit for recombinant GST tagged F11R is ~0.1ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to F11R on formalin-fixed paraffin-embedded human breast cancer tissue. [antibody concentration 1ug/ml].)

WB (Western Blot) (Western Blot analysis of F11R expression in transfected 293T cell line by F11R monoclonal antibody. Lane 1: F11R transfected lysate (32.6kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (59kD).)

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Junctional Adhesion Molecule 1, Monoclonal Antibody (Cat# AAA25432)

Full Name

Junctional Adhesion Molecule 1 (JAM-1, CD321, F11 Receptor, F11R, Junctional Adhesion Molecule A, JAMA, JCAM, KAT, Platelet Adhesion Molecule 1, PAM1, Platelet F11 Receptor, UNQ264/PRO301) (HRP)

Gene Names

F11R; JAM; KAT; JAM1; JAMA; JCAM; CD321; PAM-1

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data

IF (Immunofluorescence) (Immunofluorescence of HeLa cells using 126766 (10ug/ml).)

IHC (Immunohistochemistry) (Immunohistochemistry of formalin-fixed paraffin-embedded human tonsil using 1267661 (3ug/ml) and Immunoperoxidase.)

IP (Immunoprecipitation) (Immunoprecipitation of FGF1 transfected lysate using 126766 and Protein A Magnetic Bead and immunoblotted with FGF1 rabbit polyclonal antibody.)

WB (Western Blot) (Western Blot analysis of FGF1 expression in transfected 293T cell line using 126766. Lane 1: transfected lysate (17.5kD). Lane 2: Non-transfected lysate.)

Application Data (Detection limit for recombinant GST tagged FGF1 is ~10ng/ml using 126766 as a capture antibody.)

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FGF1, Monoclonal Antibody (Cat# AAA25685)

Full Name

FGF1 (Fibroblast Growth Factor 1, FGF-1, Acidic Fibroblast Growth Factor, aFGF, Beta-endothelial Cell Growth Factor, ECGF-beta, Heparin-binding Growth Factor 1, HBGF-1, FGFA) (PE)

Gene Names

FGF1; AFGF; ECGF; FGFA; ECGFA; ECGFB; FGF-1; HBGF1; HBGF-1; GLIO703; ECGF-beta; FGF-alpha

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western Blot Analysis of human Jurkat cell lysate using CD31 Mouse Monoclonal Antibody (C31.7).)

WB (Western Blot) (Western Blot of CD31 in THP-1 Lysate using CD31 Monoclonal Antibody (C31.7))

SDS-PAGE (SDS-PAGE Analysis Purified CD31 Mouse Monoclonal Antibody (C31.7). Confirmation of Purity and Integrity of Antibody.)

FCM (Flow Cytometry) (Flow Cytometric Analysis of paraformaldehyde-fixed Jurat cells using CD31 Mouse Monoclonal Antibody (158-2B3) followed by goat anti- Mouse- IgG-CF488 (Blue); Isotype Control (Red).)

FCM (Flow Cytometry) (Flow Cytometry of human CD31 on Jurkat Cells. Black: Cells alone; Grey: Isotype Control; Green: AF488-labeled CD31 Monoclonal Antibody (C31.7).)

FCM (Flow Cytometry) (Flow Cytometry of human CD31 on Jurkat Cells.Black: Cells alone; Green: Isotype Control; Red: PE-labeled CD31 Monoclonal Antibody (C31.7).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Angiosarcoma stained with CD31 Monoclonal Antibody (C31.7).)

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CD31 / PECAM-1, Monoclonal Antibody (Cat# AAA13801)

Full Name

CD31 / PECAM-1 (Endothelial Cell Marker) Mouse Monoclonal Antibody

Gene Names

PECAM1; CD31; PECA1; GPIIA'; PECAM-1; endoCAM; CD31/EndoCAM

Reactivity

Human, Cynomolgus Monkey, Rabbit.
Weakly reacts with Rat

Applications

Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry

Pricing

$295/0.02 mg (With BSA & Azide at 0.2mg/mL) $475/0.1 mg (With BSA & Azide at 0.2mg/mL) $475/0.1 mg (Without BSA & Azide at 1mg/mL) $905/2x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,310/3x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,690/4x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,900/5x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,900/5x0.1 mg (Without BSA & Azide at 1mg/mL)

What are Monoclonal Antibodies?

Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.

This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.

AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.

What Are The Uses of Monoclonal Antibodies

Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.

ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.

Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.

Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.

Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.

How Monoclonal Antibodies Are Used as Medicine?

Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).

Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:

• Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.


• Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.


• Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.

Why Buy Monoclonal Antibodies From Us?

At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.

1. Validated for Versatile Applications

The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).

2. Wide Selection & Specialized Options

We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.

3. High-Quality Proteins

Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.

Frequently Asked Questions

1. Are your monoclonal antibodies validated for specific applications?

Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.

2. How do I choose the right monoclonal antibody for my application?

Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.

3. How quickly can I receive my order?

Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.