Monoclonal Antibodies

Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.

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Application Data (Staining of human peripheral blood monocytes with MOUSE ANTI HUMAN CD274:RPE)

Application Data (Staining of human peripheral blood monocytes with C2549-22E4.)

Application Data (Staining of human peripheral blood monocytes with C2549-22E1.)

Application Data (Staining of human peripheral blood monocytes with C2549-22E1.)

Application Data (Jurkat cells were stained with C2549-22E (filled histogram) or isotype control (open histogram) followed by PE-conjugated anti-mouse antibody.)

Application Data (Staining of human peripheral blood monocytes with C2549-22E.)

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CD274, Monoclonal Antibody (Cat# AAA26778)

Full Name

CD274 (CD274 Antigen, B7 Homolog 1, B7H1, B7-H1, B7-H, MGC142294, MGC142296, OTTHUMP00000021029, Programmed Cell Death 1 Ligand 1, PDCD1 Ligand 1, PDCD1L1, PDCD1LG1, Programmed Death Ligand 1, PDL1, PD-L1, RGD1566211) (MaxLight 405)

Gene Names

CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1

Reactivity

Human

Applications

Flow Cytometry

Purity

Purified by protein G affinity chromatography from tissue culture supernatant.

Pricing

$830/0.1 mL $1,590/2x0.1 mL $2,305/3x0.1 mL $2,980/4x0.1 mL $3,425/5x0.1 mL

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IHC (Immunohistchemistry) (Formalin-fixed, paraffin-embedded human Uterus stained with Vimentin Monoclonal Antibody (VM1170).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Rhabdomyosarcoma stained with Vimentin Monoclonal Antibody (VM1170).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Leiomyosarcoma stained with Vimentin Monoclonal Antibody (VM1170).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Ewing's Sarcoma stained with Vimentin Monoclonal Antibody (VM1170).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Angiosarcoma stained with Vimentin Monoclonal Antibody (VM1170).)

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Melanoma stained with Vimentin Monoclonal Antibody (VM1170).)

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Vimentin, Monoclonal Antibody (Cat# AAA13829)

Full Name

Vimentin (Mesenchymal Cell Marker) Mouse Monoclonal Antibody

Gene Names

VIM; HEL113; CTRCT30

Reactivity

Human.
Does not react with Mouse and Rat

Applications

Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry

Pricing

$295/0.02 mg (With BSA & Azide at 0.2mg/mL) $475/0.1 mg (With BSA & Azide at 0.2mg/mL) $475/0.1 mg (Without BSA & Azide at 1mg/mL) $905/2x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,310/3x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,690/4x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,900/5x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,900/5x0.1 mg (Without BSA & Azide at 1mg/mL)

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Application Data (Staining of mouse peripheral blood platelets with HAMSTER ANTI MOUSE CD61:RPE)

Application Data (Staining of mouse peripheral blood platelets with HAMSTER ANTI MOUSE CD61:FITC)

Application Data (Staining of mouse peripheral blood platelets with HAMSTER ANTI MOUSE CD61:FITC)

Application Data (Staining of mouse peripheral blood platelets with HAMSTER ANTI MOUSE CD61:BIOTIN)

Application Data (Staining of mouse peripheral blood platelets with HAMSTER ANTI MOUSE CD61:Alexa Fluor®647)

Application Data (Staining of mouse peripheral blood platelets with HAMSTER ANTI MOUSE CD61:Alexa Fluor®488)

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Integrin, beta3, Monoclonal Antibody (Cat# AAA26774)

Full Name

Integrin, beta3 (ITGB3, CD61, GP3A, GPIIIa, HPA-1, NAIT, Platelet Fibrinogen Receptor beta Subunit, Platelet Glycoprotein IIIa, Platelet Membrane Glycoprotein IIIa) (PE)

Reactivity

Mouse, Rat

Applications

Flow Cytometry, Immunoprecipitation

Purity

Purified by Protein G Affinity Chromatography

Pricing

$965/0.1 mL $1,860/2x0.1 mL $2,700/3x0.1 mL $3,495/4x0.1 mL $4,055/5x0.1 mL

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Application Data (Published clone specific image: Flow cytometric analysis of AM from NO2-exposed and control rats. Rats were exposed to NO2 for the indicated times and BAL cells were stained with antibodies to ED7, ED9, RM-4, and OX-6. To overcome autofluorescence signals, primary antibodies were detected using a biotin-PE/streptavidin-anti-streptavidin enhancing system and labeling of AM was analyzed by flow cytometry following gating by help of forward and sideward scatter properties. Shown are representative results of at least six animals per group.From: Garn H, Siese A, Stumpf S, Wensing A, Renz H, Gemsa D. Phenotypical and functional characterization of alveolar macrophage subpopulations in the lungs of NO2-exposed rats. Respir Res. 2006 Jan 6;7:4.)

Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody, clone ED9 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Low power)

Application Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody, clone ED9 , red in A and Mouse anti Rat CD4 , green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power)

Application Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody, clone ED9 , red in A and Mouse anti Rat CD4 , green in B. C is the merged image with nuclei counterstained blue using DAPI. High power)

Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody, clone ED9 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. High power)

Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD172a antibody, clone ED9 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Medium power)

Application Data (Published clone specific image: Flow cytometric analysis of ED7 and ED9 expression of AM following magnetic bead separation. AM of 3 days NO2-exposed rats were separated due to their expression of the cell surface molecule ED7 using magnetic bead separation. Susbsequently, ED7 (left) and ED9 (right) expression was analyzed in unseparated AM (A), ED7-positive AM (B), and ED7-negative AM (C). Numbers right of each histogram represent the mean fluorescence of the respective cell population. The figure clearly demonstrates that ED7-positive AM show a lower ED9 expression compared to ED7-negative AM. Shown is a representative data set of more than twenty animals.From: Garn H, Siese A, Stumpf S, Wensing A, Renz H, Gemsa D. Phenotypical and functional characterization of alveolar macrophage subpopulations in the lungs of NO2-exposed rats. Respir Res. 2006 Jan 6;7:4.)

Application Data (Staining of rat peritoneal macrophages with Mouse anti Rat CD172a:RPE)

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CD172a, Monoclonal Antibody (Cat# AAA12056)

Full Name

MOUSE ANTI RAT CD172a:RPE

Gene Names

Sirpa; Bit; Ptpns1; SHPS-1

Applications

Flow Cytometry

Pricing

$655/100 Tests $1,245/2x100 Tests $1,810/3x100 Tests $2,340/4x100 Tests $2,635/5x100 Tests

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WB (Western Blot) (RUNX1 monoclonal antibody Western Blot analysis of RUNX1 expression in Hela NE.)

Application Data (Detection limit for recombinant GST tagged RUNX1 is ~1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to RUNX1 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to RUNX1 on formalin-fixed paraffin-embedded human placenta. [antibody concentration 3ug/ml].)

WB (Western Blot) (RUNX1 monoclonal antibody Western Blot analysis of RUNX1 expression in NIH/3T3.)

WB (Western Blot) (Western Blot detection against Immunogen (36.85kD).)

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RUNX1, Monoclonal Antibody (Cat# AAA24940)

Full Name

RUNX1 (Runt-related Transcription Factor 1, Acute Myeloid Leukemia 1 Protein, Core-binding Factor Subunit alpha-2, CBF-alpha-2, Oncogene AML-1, Polyomavirus Enhancer-binding Protein 2 alpha B Subunit, PEA2-alpha B, PEBP2-alpha B, SL3-3 Enhancer Factor 1 a

Gene Names

RUNX1; AML1; CBFA2; EVI-1; AMLCR1; PEBP2aB; AML1-EVI-1

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Detection of mouse Lasu1/Ureb1 by western blot. Samples: Whole cell lysate (20 ug) from BW5147.3, mIMCD-3, A20, NIH 3T3, CT26, and CH27 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Lasu1/Ureb1 recombinant monoclonal antibody (AAA23842 Lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)

WB (Western Blot) (Detection of human Lasu1/Ureb1 by western blot. Samples: Whole cell lysate (2 ug) from GaMG, K-562, Jurkat, Hep-G2, LNCaP, HeLa, HEK293T, and RKO cells prepared using NETN lysis buffer. Antibody: Rabbit anti-Lasu1/Ureb1 recombinant monoclonal antibody (AAA23842 Lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)

IP (Immunoprecipitation) (Detection of human Lasu1/Ureb1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 5% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-Lasu1/Ureb1 recombinant monoclonal antibody (AAA23842 Lot 1) used for IP at 20 ul/mg lysate. Lasu1/Ureb1 was also immunoprecipitated by other Lasu1/Ureb1 antibodies and BL672. For blotting immunoprecipitated Lasu1-Ureb1, AAA23842 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)

IHC (Immunohistochemistry) (Detection of human Lasu1/Ureb1 in FFPE head and neck squamous cell carcinoma by immunohistochemistry. Antibody: Rabbit anti-Lasu1/Ureb1 recombinant monoclonal antibody (AAA23842 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry) (Detection of human Lasu1/Ureb1 in FFPE SiHa cells by immunocytochemistry. Antibody: Rabbit anti-Lasu1/Ureb1 recombinant monoclonal antibody (AAA23842 Lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

FCM (Flow Cytometry) (Detection of human Lasu1/Ureb1 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-Lasu1/Ureb1 recombinant monoclonal antibody (AAA23842) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

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Lasu1/Ureb1, Monoclonal Recombinant Antibody (Cat# AAA23842)

Full Name

Rabbit anti-Lasu1/Ureb1 Recombinant Monoclonal Antibody [BLR129H]

Gene Names

HUWE1; MULE; Ib772; LASU1; UREB1; HECTH9; URE-B1; ARF-BP1; HSPC272

Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry

Purity

Purified

Pricing

$295/0.01 mL $680/0.1 mL $980/5x0.01 mL $1,300/2x0.1 mL $1,890/3x0.1 mL $2,440/4x0.1 mL $2,755/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with PBR antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining PBR in SW480 cells (green). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining PBR in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining PBR in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-PBR antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-PBR antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-PBR antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-PBR antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of PBR on different cell lysates using anti-PBR antibody at 1/1, 000 dilution. Positive control: Lane 1: 293T Lane 2: NIH/3T3 Lane 3: HepG2)

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PBR, Monoclonal Antibody (Cat# AAA29974)

Full Name

PBR Antibody

Gene Names

TSPO; DBI; IBP; MBR; PBR; PBS; BPBS; BZRP; PKBS; PTBR; mDRC; pk18

Reactivity

Human, Mouse

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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Application Data (Staining of mouse peripheral blood platelets with HAMSTER ANTI MOUSE CD61:RPE)

Application Data (Staining of mouse peripheral blood platelets with HAMSTER ANTI MOUSE CD61:FITC)

Application Data (Staining of mouse peripheral blood platelets with HAMSTER ANTI MOUSE CD61:FITC)

Application Data (Staining of mouse peripheral blood platelets with HAMSTER ANTI MOUSE CD61:BIOTIN)

Application Data (Staining of mouse peripheral blood platelets with HAMSTER ANTI MOUSE CD61:Alexa Fluor®647)

Application Data (Staining of mouse peripheral blood platelets with HAMSTER ANTI MOUSE CD61:Alexa Fluor®488)

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Integrin, beta3, Monoclonal Antibody (Cat# AAA26750)

Full Name

Integrin, beta3 (ITGB3, CD61, GP3A, GPIIIa, HPA-1, NAIT, Platelet Fibrinogen Receptor beta Subunit, Platelet Glycoprotein IIIa, Platelet Membrane Glycoprotein IIIa) (FITC)

Reactivity

Mouse, Rat

Applications

Flow Cytometry, Immunoprecipitation

Purity

Purified by Protein G Affinity Chromatography

Pricing

$965/0.1 mL $1,860/2x0.1 mL $2,700/3x0.1 mL $3,495/4x0.1 mL $4,055/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2OS cells using Pan-AKT antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using Pan-AKT antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of C6using Pan-AKT antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffinembedded human thyroid using Pan-AKT antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffinembedded rat testis using Pan-AKT antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using Pan-AKT antibody at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.)

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Pan-AKT, Monoclonal Antibody (Cat# AAA28311)

Full Name

Pan-AKT Rabbit mAb

Gene Names

AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity purification

Pricing

$310/0.02 mL $510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL

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Application Data

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SDS-PAGE (Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-PAX-5 (PTR1332) antibody. The HRP-conjugated Goat anti-M)

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PAX-5, Monoclonal Antibody (Cat# AAA30861)

Full Name

PAX-5 (PTR1332) Antibody

Gene Names

PAX5; BSAP

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, Immunofluorescence

Purity

Protein G

Pricing

$240/0.02 mL $315/0.05 mL $375/0.1 mL $600/0.2 mL $1,140/2x0.2 mL $1,650/3x0.2 mL $2,135/4x0.2 mL $2,350/5x0.2 mL

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Application Data (Detection limit for recombinant GST tagged PGR is ~0.3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PGR on MCF-7 cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody, 131852, to PGR on formalin-fixed paraffin-embedded human breast cancer. [antibody concentration 3ug/ml])

WB (Western Blot) (PGR monoclonal antibody, 131852, Western Blot analysis of PGR expression in MCF-7.)

WB (Western Blot) (PGR monoclonal antibody, 131852, Western Blot analysis of PGR expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen, 131852 (37.84kD))

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Progesterone Receptor, Monoclonal Antibody (Cat# AAA25215)

Full Name

Progesterone Receptor (PgR, PR, Nuclear Receptor Subfamily 3 Group C Member 3, NR3C3) (FITC)

Gene Names

PGR; PR; NR3C3

Reactivity

Human, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded esophagus cancer tissues using ZFP42 mouse mAb with DAB staining.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using ZFP42 mouse mAb with DAB staining.)

FCM (Flow Cytometry) (Flow cytometric analysis of HEK293 cells using ZFP42 mouse mAb (green) and negative control (red).)

WB (Western Blot) (Western blot analysis using ZFP42 mouse mAb against Jurkat (1), HEK293 (2), Raji (3) and PC-3 (4) cell lysate.)

WB (Western Blot) (Western blot analysis using ZFP42 mAb against HEK293 (1) and ZFP42 (AA)

WB (Western Blot) (Western blot analysis using ZFP42 mAb against human ZFP42 recombinant protein. (Expected MW is 32.7 kDa))

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ZFP42, Monoclonal Antibody (Cat# AAA14133)

Full Name

Anti-ZFP42 Mouse mAb

Gene Names

ZFP42; REX1; REX-1; ZNF754; zfp-42

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Pricing

$565/0.1 mL $1,070/2x0.1 mL $1,545/3x0.1 mL $1,900/5x0.1 mL $1,995/4x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded bladder cancer tissues using CCNA2 mouse mAb with DAB staining.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using CCNA2 mouse mAb with DAB staining.)

FCM (Flow Cytometry) (Flow cytometric analysis of A431 cells using CCNA2 mouse mAb (green) and negative control (red).)

WB (Western Blot) (Western blot analysis using CCNA2 mouse mAb against Hela (1), HEK293 (2), Jurkat (3), K562 (4), SK-Br-3 (5), NIH/3T3 (6) cell lysate.)

WB (Western Blot) (Western blot analysis using CCNA2 mAb against HEK293 (1) and CCNA2 (AA)

WB (Western Blot) (Western blot detection of Cyclin A2 in Jurkat cell lysates using Cyclin A2 mouse mAb (1:1000 diluted).Predicted band size:50KDa.Observed band size:50KDa.)

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Cyclin A2, Monoclonal Antibody (Cat# AAA14164)

Full Name

Anti-Cyclin A2 Mouse mAb

Gene Names

CCNA2; CCN1; CCNA

Reactivity

Human

Applications

Western Blot

Pricing

$565/0.1 mL $1,070/2x0.1 mL $1,545/3x0.1 mL $1,900/5x0.1 mL $1,995/4x0.1 mL

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WB (Western Blot) (Western Blot analysis of HMGB1 expression in transfected 293T cell line by HMGB1 monoclonal antibody (M03), clone 1B11.Lane 1: HMGB1 transfected lysate (25 KDa).Lane 2: Non-transfected lysate.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HMGB1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HMGB1 on HeLa cell. [antibody concentration 10 ug/ml])

Application Data (Detection limit for recombinant GST tagged HMGB1 is approximately 3ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HMGB1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to HMGB1 on formalin-fixed paraffin-embedded human tonsil. [antibody concentration 3 ug/ml])

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HMGB1, Monoclonal Antibody (Cat# AAA26248)

Full Name

HMGB1 (High-Mobility Group Box 1, DKFZp686A04236, HMG1, HMG3, SBP-1) (Biotin)

Gene Names

HMGB1; HMG1; HMG3; HMG-1; SBP-1

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Radixin antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining Radixin in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-Radixin antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Radixin antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Radixin antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Radixin antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Radixin on different lysates using anti-Radixin antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: A431 Lane 3: HepG2)

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Radixin, Monoclonal Antibody (Cat# AAA30121)

Full Name

Radixin Antibody

Gene Names

RDX; DFNB24

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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ICC (Immunocytochemistry) (ICC staining mtTFA in SK-Br-3 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining mtTFA in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining mtTFA in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-mtTFA antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-mtTFA antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-mtTFA antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of mtTFA on Hela cell using anti-mtTFA antibody at 1/1, 000 dilution.)

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mtTFA, Monoclonal Antibody (Cat# AAA30378)

Full Name

mtTFA Antibody

Gene Names

TFAM; TCF6; MTTF1; MTTFA; TCF6L1; TCF6L2; TCF6L3

Reactivity

Human

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD204: Alexa Fluor 488)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD204: Low Endotoxin)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD204 antibody, clone 2F8 followed by horseradish peroxidase Goat anti Rat IgG antibody . Low power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD204 antibody, clone 2F8 followed by horseradish peroxidase Goat anti Rat IgG antibody . Medium power)

Application Data (Staining of mouse peritoneal macrophages cells with Rat anti Mouse CD204:FITC)

Application Data (Western Blot staining of J774 lysate (non reduced) probed with Rat anti Mouse CD204)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD204: Biotin)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD204 antibody, clone 2F8 followed by horseradish peroxidase Goat anti Rat IgG antibody . High power)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD204: Alexa Fluor 647)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD204:FITC)

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CD204, Monoclonal Antibody (Cat# AAA12076)

Full Name

RAT ANTI MOUSE CD204:FITC

Gene Names

Msr1; MSR; Scvr; MRS-A; MSR-A; SR-AI; SR-AII; Scara1

Applications

Flow Cytometry

Pricing

$775/0.5 mg $1,480/2x0.5 mg $2,155/3x0.5 mg $2,785/4x0.5 mg $3,170/5x0.5 mg

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IF (Immunofluorescence) (Immunofluorescence analysis of PC-12 cells using MCM2 Rabbit mAb (AAA28544) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using MCM2 Rabbit mAb (AAA28544) at dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat spleen tissue using MCM2 Rabbit mAb (AAA28544) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using MCM2 Rabbit mAb (AAA28544) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using MCM2 Rabbit mAb (AAA28544) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using MCM2 Rabbit mAb (AAA28544) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human thyroid tissue using MCM2 Rabbit mAb (AAA28544) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Human stomach tissue using MCM2 Rabbit mAb (AAA28544) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human esophagus tissue using MCM2 Rabbit mAb (AAA28544) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using MCM2 Rabbit mAb (AAA28544) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using MCM2 Rabbit mAb (AAA28544) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using MCM2 Rabbit mAb (AAA28544) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

WB (Western Blot) (Western blot analysis of various lysates using MCM2 Rabbit mAb (AAA28544) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 0.3s.)

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MCM2, Monoclonal Antibody (Cat# AAA28544)

Full Name

MCM2 Rabbit mAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Affinity purification

Pricing

$285/0.02 mL $465/0.1 mL $885/2x0.1 mL $1,275/3x0.1 mL $1,650/4x0.1 mL $1,765/5x0.1 mL

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WB (Western Blot) (Detection of human B7-H4 by western blot. Samples: Whole cell lysate (50 ug) from OVCAR-4, Hep-G2, MCF-7, HeLa, HEK293T, Jurkat, MOLT-4, HH, and C5/MJ cells prepared using NETN lysis buffer. Antibody: Rabbit anti-B7-H4 recombinant monoclonal antibody (AAA23811 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 seconds.)

IP (Immunoprecipitation) (Detection of human B7-H4 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from OVCAR-4 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-B7-H4 recombinant monoclonal antibody (AAA23811 lot 1) used for IP at 20 ul per reaction. B7-H4 was also immunoprecipitated by rabbit anti-B7-H4 recombinant monoclonal antibody [209-2B5]. For blotting immunoprecipitated B7-H4, AAA23811 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)

IHC (Immunohistochemistry) (Detection of human B7-H4 (red) by immunohistochemistry. Sample: FFPE section of human lung carcinoma. Antibody: Rabbit anti-B7-H4 recombinant monoclonal antibody (AAA23811 lot 1) used at 1:250. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: Opal. Counterstain: DAPI (blue).)

IHC (Immunohistochemistry) (Detection of human B7-H4 by immunohistochemistry. Sample: FFPE section of ovarian carcinoma. Antibody: Rabbit anti-B7-H4 recombinant monoclonal antibody (AAA23811 lot 1) used at 1:100. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry) (Detection of human B7-H4 by immunocytochemistry. Sample: FFPE section of OVCAR-3 cells. Antibody: Rabbit anti-B7-H4 recombinant monoclonal antibody (AAA23811 lot 1) used at 1:100. Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

FCM (Flow Cytometry) (Detection of human B7-H4 (shaded) in human peripheral blood mononuclear cells by flow cytometry. Antibody: Rabbit anti-B7-H4 recombinant monoclonal (AAA23811 lot 1) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)

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B7-H4, Monoclonal Recombinant Antibody (Cat# AAA23811)

Full Name

Rabbit anti-B7-H4 Recombinant Monoclonal Antibody [BLR043F]

Gene Names

VTCN1; B7X; B7H4; B7S1; B7-H4; B7h.5; VCTN1; PRO1291

Reactivity

Human

Applications

Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Immunohistochemistry, Flow Cytometry

Purity

Purified

Pricing

$295/0.01 mL $680/0.1 mL $980/5x0.01 mL $1,300/2x0.1 mL $1,890/3x0.1 mL $2,440/4x0.1 mL $2,755/5x0.1 mL

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Application Data

Application Data

Application Data

Application Data

Application Data

Application Data (Staining of human peripheral blood lymphocytes with C2253-02D.)

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CD2, Monoclonal Antibody (Cat# AAA26777)

Full Name

CD2 (CD2 Antigen, LFA3 Receptor, Lymphocyte Function Antigen 2, LFA2, p50, Rosette Receptor, Sheep Erythrocyte Receptor, Sheep Red Blood Cell Receptor, SRBC, T Cell Surface Antigen CD2, T Cell Surface Antigen T11/Leu-5, T11) (MaxLight 405)

Reactivity

Human, Mouse

Applications

Flow Cytometry, Immunohistochemistry, Immunoprecipitation

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$950/0.1 mL $1,830/2x0.1 mL $2,660/3x0.1 mL $3,440/4x0.1 mL $3,985/5x0.1 mL

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Application Data (Published customer image: Increased accumulation of repair-associated macrophages surrounding collaterals in ischemic hind limbs is PAR2-dependent. (A) Stainings of CD206-positive macrophages (green) and SMA-positive vessels (red) in non-ischemic (control) and ischemic (ligated) hind limbs of WT, PAR1-/- and PAR2-/- mice are shown. Nuclei were visualized with DAPI (blue). Arrows indicate single macrophages in the non-ischemic adductor. Quantification of the average number of repair-associated macrophages per vessel is indicated on the right. (B) Correlation between the number of CD206-positive macrophages in the ischemic tissues and the expression of CD11b and (C) CD115 on monocytes. ** p)

Application Data (Published customer image: Western blot analysis to determine type of activation of adult primary microglial cells produced from wild-type and IL-1 KO mice stimulated with IL-4, IL-13 or IL-4/IL-13 with or without IL-1beta. (A) Representative western blotting data of primary microglial cells produced from wild-type (wild) and IL-1 KO mice and exposed for 24 hours to IL-4, IL-13 or IL-4 plus IL-13 (IL-4/IL-13) with or without IL-1beta. Each lane expected to CD206 blotting were applied 7 ug of reduced samples. Non-reduced samples (5 ug) were applied to detect CD206. Densitometric analysis of COX2 (B), Ym1 (C), Arg-1 (D) and CD206 (E) (n = 3 each group). (B) COX2 levels are increased by exposure of cells to IL-1beta and are not influenced by IL-4 or IL-13 alone. The COX2 level was slightly enhanced by IL-1beta and IL-4 co-treatment. (C) Ym1 levels are increased by exposure of cells to IL-4 and IL-4/Il-13 and are synergistically increased by co-treatment with IL-1beta. However, only a low level of Ym1 is seen upon exposure of cells to IL-13, and is significantly less than that seen in response to exposure of cells to IL-4. (D) Arg-1 shows similar levels in response to exposure to IL-4 and IL-4/IL-13; these are synergistically increased by co-treatment with IL-1beta. However, low levels of Arg-1 are seen for exposure of cells to IL-13. (G) CD206 was detected in response to exposure of cells to both IL-4 and IL-4/IL-13 with or without IL-1beta; however, CD206 levels in IL-13-exposed samples were lower than those seen with the other treatments. Data are expressed as mean +/- SD (n = 3). *: P < 0.05, **: P < 0.01, ***: P < 0.001 compared with the IL-4-treated group without IL-1beta for each genotype (one-way ANOVA followed by Dunnett post-hoc test). ANOVA, analysis of variance; arg-1, arginase 1; COX 2, cyclooxygenase 2.From: Sato A, Ohtaki H, Tsumuraya T, Song D, Ohara K, Asano M, Iwakura Y, Atsumi T, Shioda S. Interleukin-1 participates in the classical and alternative activation of microglia/macrophages after spinal cord injury. J Neuroinflammation. 2012 Apr 7;9:65.)

Application Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained in blue using DAPI. Low power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . High power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Low power)

Application Data (Staining of J774 cell line with Rat anti Mouse CD206 following permeabilisation with Leucoperm)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD206: Alexa Fluor 488)

Application Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 , green in A and Rat anti Mouse CD8 antibody, clone YTS105.18 , red in B. C is the merged image with nuclei counterstained in blue using DAPI. High power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD206 antibody, clone MR5D3 followed by horseradish peroxidase conjugated Goat anti Rat IgG antibody . Medium power)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD206: Alexa Fluor 647)

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CD206, Monoclonal Antibody (Cat# AAA12117)

Full Name

RAT ANTI MOUSE CD206:Biotin

Gene Names

Mrc1; MR; CD206; AW259686

Applications

Flow Cytometry

Pricing

$585/0.1 mg $1,110/2x0.1 mg $1,610/3x0.1 mg $2,075/4x0.1 mg $2,295/5x0.1 mg

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Application Data (Detection limit for recombinant GST tagged CSE1L is approximately 0.1ng/ml as a capture antibody.)

WB (Western Blot) (CSE1L monoclonal antibody (M04), clone 2F4 Western Blot analysis of CSE1L expression in Hela S3 NE.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CSE1L on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CSE1L on HeLa cell. [antibody concentration 10 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CSE1L on formalin-fixed paraffin-embedded human testis. [antibody concentration 6 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to CSE1L on formalin-fixed paraffin-embedded human testis. [antibody concentration 6 ug/ml])

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CSE1L, Monoclonal Antibody (Cat# AAA26156)

Full Name

CSE1L (CSE1 Chromosome Segregation 1-like (yeast), CAS, CSE1, MGC117283, MGC130036, MGC130037, XPO2) (Biotin)

Gene Names

CSE1L; CAS; CSE1; XPO2

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged PTBP1 is ~0.1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of PTBP1 transfected lysate using PTBP1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PTBP1 rabbit polyclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PTBP1 on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PTBP1 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of PTBP1 expression in transfected 293T cell line by PTBP1 monoclonal antibody, Lane 1: PTBP1 transfected lysate (59.6kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (PTBP1 monoclonal antibody, Western Blot analysis of PTBP1 expression in A-431.)

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PTBP1, Monoclonal Antibody (Cat# AAA24923)

Full Name

PTBP1 (Polypyrimidine Tract-binding Protein 1, PTB, Heterogeneous Nuclear Ribonucleoprotein I, hnRNP I, 57kD RNA-binding Protein PPTB-1, MGC10830, MGC8461) (Biotin)

Gene Names

PTBP1; PTB; PTB2; PTB3; PTB4; pPTB; HNRPI; PTB-1; PTB-T; HNRNPI; HNRNP-I

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of CSTF3 expression in transfected 293T cell line by CSTF3 monoclonal antibody. Lane 1: CSTF3 transfected lysate (11.44kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western blot analysis of CSTF3 over-expressed 293 cell line, cotransfected with CSTF3 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with CSTF3 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged CSTF3 is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CSTF3 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to CSTF3 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot detection against Immunogen (37.07kD).)

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CSTF3, Monoclonal Antibody (Cat# AAA25657)

Full Name

CSTF3 (Cleavage Stimulation Factor Subunit 3, CF-1 77kD Subunit, Cleavage Stimulation Factor 77kD Subunit, CSTF 77kD Subunit, CstF-77) (PE)

Gene Names

CSTF3; CSTF-77

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded Pancreas. 1. Antibody was diluted at 1:200(4 degree overnight). 2. Citrate buffer of pH6.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Pancreas. 1. Antibody was diluted at 1:200(4 degree overnight). 2. Citrate buffer of pH6.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Pancreas. 1. Antibody was diluted at 1:200(4 degree overnight). 2. Citrate buffer of pH6.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded Pancreatic carcinoma. 1. Antibody was diluted at 1:200(4 degree overnight). 2. Citrate buffer of pH6.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Pancreatic carcinoma. 1. Antibody was diluted at 1:200(4 degree overnight). 2. Citrate buffer of pH6.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Pancreatic carcinoma. 1. Antibody was diluted at 1:200(4 degree overnight). 2. Citrate buffer of pH6.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Pancreatic carcinoma. 1. Antibody was diluted at 1:200(4 degree overnight). 2. Citrate buffer of pH6.0 was used for antigen retrieval. 3.Secondary antibody was diluted at 1:200(room temperature. 30min).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human pancreas Antibody was diluted at 1:200(4 degree overnight).)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human pancreas Antibody was diluted at 1:200(4 degree overnight).)

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Insulin, Monoclonal Antibody (Cat# AAA30788)

Full Name

Human Insulin (PT2015) Mouse Monoclonal Antibody

Gene Names

INS; ILPR; IRDN; IDDM2; MODY10

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry

Purity

The antibody was affinity-purified from mouse ascites by affinity-chromatography using specific immunogen.

Pricing

$325/0.05 mL $445/0.1 mL $685/0.2 mL $1,310/2x0.2 mL $1,905/3x0.2 mL $2,460/4x0.2 mL $2,760/5x0.2 mL

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WB (Western Blot) (Western blot analysis of PDK2 over-expressed 293 cell line, cotransfected with PDK2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PDK2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged PDK2 is ~0.3ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PDK2 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 0.8ug/ml])

WB (Western Blot) (Western Blot analysis of PDK2 expression in transfected 293T cell line by PDK2 monoclonal antibody. Lane 1: PDK2 transfected lysate (46.2kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (PDK2 monoclonal antibody Western Blot analysis of PDK2 expression in U-2 OS.)

WB (Western Blot) (Western Blot detection against Immunogen (35.31kD).)

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PDHK2, Monoclonal Antibody (Cat# AAA25489)

Full Name

PDHK2 [Pyruvate Dehydrogenase [Lipoamide]] Kinase Isozyme 2, Mitochondrial, Pyruvate Dehydrogenase Kinase Isoform 2, PDH Kinase 2, PDKII, PDK2) (HRP)

Gene Names

PDK2; PDHK2; PDKII

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (BPNT1 monoclonal antibody Western Blot analysis of BPNT1 expression in HeLa.)

Application Data (Detection limit for recombinant GST tagged BPNT1 is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to BPNT1 on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to BPNT1 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of BPNT1 expression in transfected 293T cell line by BPNT1 monoclonal antibody. Lane 1: BPNT1 transfected lysate (28.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

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BPNT1, Monoclonal Antibody (Cat# AAA24148)

Full Name

BPNT1 (3'(2'),5'-bisphosphate nucleotidase 1, Bisphosphate 3'-nucleotidase 1, PAP-inositol 1,4-phosphatase) (AP)

Gene Names

BPNT1; PIP; HEL20

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of SH-SY-5Y cells with MAP1LC3A antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining MAP1LC3A in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining MAP1LC3A in PC12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining MAP1LC3A in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse brain tissue using anti-MAP1LC3A antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-MAP1LC3A antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-MAP1LC3A antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of MAP1LC3A on different lysates using anti-MAP1LC3A antibody at 1/1, 000 dilution. Positive control: Lane 1: SHG-44 Lane 2: Mouse brain Lane 3: Mouse liver Lane 4: Mouse skeletal muscle)

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MAP1LC3A, Monoclonal Antibody (Cat# AAA30089)

Full Name

MAP1LC3A Antibody

Gene Names

MAP1LC3A; LC3; LC3A; ATG8E; MAP1ALC3; MAP1BLC3

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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Application Data

Application Data

Application Data

Application Data

Application Data

SDS-PAGE (Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CD8 a(ABT155) antibody. The HRP-conjugated Goat anti-M)

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CD8 a, Monoclonal Antibody (Cat# AAA30877)

Full Name

CD8 a (ABT155) Antibody

Gene Names

CD8A; CD8; MAL; p32; Leu2

Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Immunofluorescence

Purity

Protein G

Pricing

$240/0.02 mL $315/0.05 mL $375/0.1 mL $600/0.2 mL $1,140/2x0.2 mL $1,650/3x0.2 mL $2,135/4x0.2 mL $2,350/5x0.2 mL

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FCM (Flow Cytometry) (Flow cytometry analysis of PD-L2 overexpressing HEK293 cells using PD-L2 antibody and control mouse IgG antibody at 10 μg/ml. Blue: Untransfected HEK293 cells. Yellow: PD-L2 overexpressing HEK293 cells.)

IHC (Immunohistochemistry) (Immunohistochemistry of PD-L2 in human colon carcinoma tissue with PD-L2 antibody at 2 μg/mL.)

IHC (Immunohistchemistry) (Immunohistochemistry of PD-L2 in human tonsil tissue with PD-L2 antibody at 2 μg/mL.)

IF (Immunofluorescence) (Immunofluorescence of PD-L2 in human colon carcinoma tissue with PD-L2 antibody at 20 μg/mL.Green: PDL2 Antibody [8C12]Blue: DAPI staining)

IF (Immunofluorescence) (Immunofluorescence of PD-L2 in human tonsil tissue with PD-L2 antibody at 20 μg/mL.Green: PDL2 Antibody [8C12]Blue: DAPI staining)

IF (Immunofluorescence) (Immunofluorescence of PD-L2 in transfected HEK293 cells with PD-L2 antibody at 20 μg/mL.Green: PDL2 Antibody [8C12]Blue: DAPI staining)

ICC (Immunocytochemistry) (Immunocytochemistry of PD-L2 in transfected HEK293 cells with PD-L2 antibody at 5 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 5 μg/mL.)

WB (Western Blot) (Western blot analysis of PD-L2 in overexpressing HEK293 cells PD-L2 antibody at 0.5 and 1 μg/ml)

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PDL2, Monoclonal Antibody (Cat# AAA10986)

Full Name

PDL2 Antibody [8C12]

Gene Names

PDCD1LG2; B7DC; Btdc; PDL2; CD273; PD-L2; PDCD1L2; bA574F11.2

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Protein A purified

Pricing

$570/0.1 mg $1,080/2x0.1 mg $1,560/3x0.1 mg $2,015/4x0.1 mg $2,295/5x0.1 mg

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ELISA (Titration curve analysis of B7-H3 mAbs to detect recombinant B7-H3 in ELISA at decreasing concentrations.)

FCM (Flow Cytometry) (Flow cytometry analysis of B7-H3 in HEK293 cells using B7-H3 antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: B7-H3 over expressing HEK293 cells.)

IHC (Immunohistochemistry) (Immunohistochemistry of B7-H3 in human colon carcinoma tissue using B7-H3 Antibody and control mouse IgG (corner box) at 2 μg/ml.)

IF (Immunofluorescence) (Immunofluorescence of B7-H3 in HEK293 cells using B7-H3 Antibody at 5 μg/ml.Green: B7-H3 Antibody [2H5]Blue: DAPI staining)

ICC (Immunocytochemistry) (Immunocytochemistry of B7-H3 in HEK293 cells using B7-H3 antibody and control mouse IgG antibody (left corner box) at 1 μg/ml.)

WB (Western Blot) (Western blot analysis of B7-H3 in HEK293 cells using B7-H3 antibody at (A) 0.25 (B) 0.5 and (C) 1 μg/ml.)

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B7-H3, Monoclonal Antibody (Cat# AAA11022)

Full Name

B7-H3 Antibody [2H5]

Gene Names

CD276; B7H3; B7-H3; B7RP-2; 4Ig-B7-H3

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Protein A purified

Pricing

$570/0.1 mg $1,080/2x0.1 mg $1,560/3x0.1 mg $2,015/4x0.1 mg $2,295/5x0.1 mg

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WB (Western Blot) (Western Blot analysis of GLUL expression in transfected 293T cell line by GLUL monoclonal antibody (M02), clone 3B6.Lane 1: GLUL transfected lysate (42.1 KDa).Lane 2: Non-transfected lysate.)

WB (Western Blot) (GLUL monoclonal antibody (M02), clone 3B6. Western Blot analysis of GLUL expression in Raw 264.7 (Cat # L024V1).)

WB (Western Blot) (GLUL monoclonal antibody (M02), clone 3B6. Western Blot analysis of GLUL expression in PC-12 (Cat # L012V1).)

Application Data (Detection limit for recombinant GST tagged GLUL is approximately 0.03ng/ml as a capture antibody.)

WB (Western Blot) (GLUL monoclonal antibody (M02), clone 3B6. Western Blot analysis of GLUL expression in HepG2 (Cat # L019V1).)

WB (Western Blot) (GLUL monoclonal antibody (M02), clone 3B6 Western Blot analysis of GLUL expression in Jurkat (Cat # L017V1).)

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GLUL, Monoclonal Antibody (Cat# AAA26426)

Full Name

GLUL (Glutamate-Ammonia Ligase (Glutamine Synthetase), GLNS, GS, PIG43, PIG59) (HRP)

Gene Names

GLUL; GS; GLNS; PIG43; PIG59

Applications

Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (AKAP8 monoclonal antibody (Western Blot analysis of AKAP8 expression in HeLa NE)

IP (Immunoprecipitation) (Immunoprecipitation of AKAP8 transfected lysate using AKAP8 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with AKAP8 monoclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to AKAP8 on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to AKAP8 on formalin-fixed paraffin-embedded human pancreas. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of AKAP8 expression in transfected 293T cell line by AKAP8 monoclonal antibody Lane 1: AKAP8 transfected lysate (76.2kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (38.43kD).)

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AKAP8, Monoclonal Antibody (Cat# AAA25607)

Full Name

AKAP8 (A-kinase Anchor Protein 8, AKAP-8, A-kinase Anchor Protein 95kD, AKAP 95, AKAP95, DKFZp586B1222) (PE)

Gene Names

AKAP8; AKAP-8; AKAP95; AKAP 95; AKAP-95

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (TUBB2A monoclonal antibody (M04), clone 3B2. Western Blot analysis of TUBB2A expression in NIH/3T3.)

WB (Western Blot) (TUBB2A monoclonal antibody (M04), clone 3B2. Western Blot analysis of TUBB2A expression in Jurkat.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TUBB2A on formalin-fixed paraffin-embedded human colon. [antibody concentration 1.5 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TUBB2A on formalin-fixed paraffin-embedded human colon. [antibody concentration 1.5 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TUBB2A on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TUBB2A on HeLa cell. [antibody concentration 10 ug/ml])

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TUBB2A, Monoclonal Antibody (Cat# AAA26276)

Full Name

TUBB2A (Tubulin, beta 2A, TUBB, TUBB2, dJ40E16.7) (Biotin)

Gene Names

TUBB2A; TUBB; TUBB2; CDCBM5

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Published customer image: Representative images of the inflammatory changes in the facial nucleus during axonal regeneration, one week following facial nerve transaction. a, b: CD11b immunoreactivity for microglia is increased in the axotomized facial nucleus, and microglia enwrap the facial motor neurons, e.g. at arrows. The regenerating neurons were retrogradely labelled with fluorogold. c, d: CD6- positive T-cells accumulated in the injured motor nucleus (arrows). They had little cytoplasm but dense nuclei (c) and were sometimes clustered around neurons retrogradely labelled with fluorogold (d). The scale bar in (a) also applies to (b) and that in (c) also applies to (d).From: Shokouhi et al. BMC Neuroscience 2010 11:13.)

Application Data (Published customer image: CD11b immunoreactive (red) microglia in the motor cortex following cervical corticospinal tract injury. Representative images of motor cortex - a, b: one week (deconvolved images); c, d: two weeks following corticospinal tract injury. The corticospinal neurons were retrogradely labelled with fluorogold applied to the spinal cord at the time of injury. There is little sign of microglial activation. The inset in (c) shows a CD6-positive T-cell in the motor cortex, but no accumulation of such cells was detected.From: Shokouhi BN, Wong BZ, Siddiqui S, Lieberman AR, Campbell G, Tohyama K, Anderson PN. Microglial responses around intrinsic CNS neurons are correlated with axonal regeneration. BMC Neurosci. 2010 Feb 5;11:13.)

Application Data (Staining of rat peritoneal macrophages with Mouse anti Rat CD11b)

Application Data (Published customer image: Microglia in the red nucleus (a-d and inset) and motor cortex (e, f) 4 weeks after nerve graft implantation into the rubrospinal tract and cervical dorsal columns respectively. Neurons with regenerating axons were retrogradely labelled with fluorogold. Microglia established close contacts with regenerating rubrospinal neurons (arrows), which also expressed ATF3 (inset). No retrogradely labelled corticospinal neurons were detected (e and f) and there was no microglial activation in the motor cortex. (a-c) are non-deconvolved and (d-f plus inset) are deconvolved images. The scale bar in (a) also applies to (b).From: Shokouhi BN, Wong BZ, Siddiqui S, Lieberman AR, Campbell G, Tohyama K, Anderson PN. Microglial responses around intrinsic CNS neurons are correlated with axonal regeneration. BMC Neurosci. 2010 Feb 5;11:13.)

Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD11b antibody followed by horseradish peroxidase conjugated Goat anti Mouse IgG2a as a detection reagent. High power)

Application Data (Staining of rat peritoneal macrophages cells with Mouse anti Rat CD11b:Alexa Fluor 488)

Application Data (Published customer image: Rubrospinal injury produces little microglial activation and no accumulation of T-cells in the red nucleus. a, b: Representative images show there is little difference in CD11b immunoreactivity for microglia in the red nucleus (*) one week following axotomy. c, d: confocal images of beta thymosin immunoreactive microglia in the red nucleus 3 weeks after injury. e: a rare CD6-positive T lymphocyte in the red nucleus of an unoperated rat. f: one week after injury no T lymphocytes can be identified in the red nucleus around the axotomized rubrospinal neurons, which can be recognised by their expression of ATF3 (green). Neuronal cytoplasm has been visualised by high gain in the red signal; this does not represent CD6 signal.From: Shokouhi BN, Wong BZ, Siddiqui S, Lieberman AR, Campbell G, Tohyama K, Anderson PN. Microglial responses around intrinsic CNS neurons are correlated with axonal regeneration. BMC Neurosci. 2010 Feb 5;11:13.)

Application Data (Staining of rat peritoneal macrophages with Mouse anti Rat CD11b:RPE-Alexa Fluor 647)

Application Data (Immunofluoescence staining of rat lymph node cryosection with Mouse anti Rat CD11b antibody , red in A and Mouse anti Rat CD8 , green in B. C is the merged image with nuclei counter-stained in blue using DAPI. High power)

Application Data (Immunofluoescence staining of rat lymph node cryosection with Mouse anti Rat CD11b antibody , red in A and Mouse anti Rat CD8 , green in B. C is the merged image with nuclei counter-stained in blue using DAPI. Low power)

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CD11b, Monoclonal Antibody (Cat# AAA11853)

Full Name

MOUSE ANTI RAT CD11b:Biotin

Gene Names

ITGAM; CD11B

Applications

Immunohistochemistry, Flow Cytometry

Pricing

$555/0.1 mg $1,045/2x0.1 mg $1,510/3x0.1 mg $1,955/4x0.1 mg $2,165/5x0.1 mg

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ICC (Immunocytochemistry) (ICC staining HAPLN1 in BT-20 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining HAPLN1 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining HAPLN1 in HUVEC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-HAPLN1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-HAPLN1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-HAPLN1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HAPLN1 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of HAPLN1 on different lysates using anti-HAPLN1 antibody at 1/1, 000 dilution. Positive control: Lane 1: BT-20 Lane 2: HUVEC Lane 3: SW480)

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HAPLN1, Monoclonal Antibody (Cat# AAA30063)

Full Name

HAPLN1 Antibody

Gene Names

HAPLN1; CRTL1

Reactivity

Human, Mouse, Rat, Zebrafish

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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Application Data (Staining of NALM6 cells with MOUSE ANTI HUMAN CD10:ALEXA 488)

Application Data (Staining of human peripheral blood granulocytes with MOUSE ANTI HUMAN CD10:RPE)

Application Data (Staining of human peripheral blood granulocytes with MOUSE ANTI HUMAN CD10:FITC)

Application Data (Staining of human peripheral blood granulocytes with MOUSE ANTI HUMAN CD10:BIOTIN)

Application Data (Staining of human peripheral blood granulocytes with MOUSE ANTI HUMAN CD10:AZIDE FREE)

Application Data (Staining of human peripheral blood granulocytes with MOUSE ANTI HUMAN CD10:APC)

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CD10, Monoclonal Antibody (Cat# AAA26775)

Full Name

CD10 (CALLA, CD 10, Common Acute Lymphocytic Leukemia Antigen, Enkephalinase, gp100, Membrane Metalloendopeptidase, MME, Neprilysin, Neutral Endopeptidase, Pmel17) (MaxLight 405)

Gene Names

MME; NEP; SFE; CD10; CALLA

Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Purified by protein G affinity chromatography from tissue culture supernatant.

Pricing

$950/0.1 mL $1,830/2x0.1 mL $2,660/3x0.1 mL $3,440/4x0.1 mL $3,985/5x0.1 mL

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WB (Western Blot) (FLJ20097 monoclonal antibody, Western Blot analysis of FLJ20097 expression in HeLa.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FLJ20097 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FLJ20097 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml].)

WB (Western Blot) (FLJ20097 monoclonal antibody. Western Blot analysis of FLJ20097 expression in NIH/3T3.)

WB (Western Blot) (FLJ20097 monoclonal antibody. Western Blot analysis of FLJ20097 expression in human pancreas.)

WB (Western Blot) (Western Blot detection against Immunogen (37.44kD).)

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CCDC132, Monoclonal Antibody (Cat# AAA25101)

Full Name

CCDC132 (Coiled-coil Domain-containing Protein 132, KIAA1861, DKFZp313I2429, FLJ20097, FLJ23581, KIAA1861, MGC176659) (FITC)

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged YY1 is approximately 0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to YY1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to YY1 on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (YY1 monoclonal antibody (M03), clone 4D2 Western Blot analysis of YY1 expression in Hela S3 NE.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to YY1 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to YY1 on formalin-fixed paraffin-embedded human endometrium. [antibody concentration 3 ug/ml])

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YY1, Monoclonal Antibody (Cat# AAA26175)

Full Name

YY1 (YY1 Transcription Factor, DELTA, INO80S, NF-E1, UCRBP, YIN-YANG-1) (Biotin)

Gene Names

YY1; DELTA; NF-E1; UCRBP; GADEVS; INO80S; YIN-YANG-1

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (TXNDC4 monoclonal antibody Western Blot analysis of TXNDC4 expression in K-562.)

WB (Western Blot) (TXNDC4 monoclonal antibody Western Blot analysis of TXNDC4 expression in A-431)

Application Data (Detection limit for recombinant GST tagged TXNDC4 is 1ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of TXNDC4 transfected lysate using TXNDC4 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with TXNDC4 rabbit polyclonal antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TXNDC4 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of TXNDC4 expression in transfected 293T cell line by TXNDC4 monoclonal antibody Lane 1: TXNDC4 transfected lysate (47kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (67.58kD).)

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Thioredoxin Domain-containing Protein 4, Monoclonal Antibody (Cat# AAA24398)

Full Name

Thioredoxin Domain-containing Protein 4 (TXNDC4, Endoplasmic Reticulum Resident Protein 44, ER Protein 44, ERp44, KIAA0573, PDIA10, UNQ532/PRO1075) (AP)

Gene Names

ERP44; PDIA10; TXNDC4

Reactivity

Human

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (AKT1 monoclonal antibody (M08), clone 1D4. Western Blot analysis of AKT1 expression in Raw 264.7.)

WB (Western Blot) (AKT1 monoclonal antibody (M08), clone 1D4. Western Blot analysis of AKT1 expression in NIH/3T3.)

Application Data (Detection limit for recombinant GST tagged AKT1 is approximately 1ng/ml as a capture antibody.)

WB (Western Blot) (AKT1 monoclonal antibody (M08), clone 1D4 Western Blot analysis of AKT1 expression in Jurkat.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to AKT1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to AKT1 on HeLa cell. [antibody concentration 10 ug/ml])

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AKT1, Monoclonal Antibody (Cat# AAA26184)

Full Name

AKT1 (v-akt murine Thymoma Viral Oncogene Homolog 1, AKT, MGC99656, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA) (Biotin)

Gene Names

AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Applications

Immunofluorescence, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Detection of mouse PU.1 by western blot. Samples: Whole cell lysate (50 ug) from NIH 3T3, CT26, RAW 264.7, TCMK-1, and BW5147.3 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PU.1 recombinant monoclonal antibody (AAA23854 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 3 minutes. Lower Panel: Rabbit anti-Actin recombinant monoclonal antibody .)

IP (Immunoprecipitation) (Detection of mouse PU.1 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from RAW 264.7 cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PU.1 recombinant monoclonal antibody (AAA23854 lot 1) used at 6 ul per reaction. For blotting immunoprecipitated PU.1, AAA23854 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 minutes.)

IHC (Immunohistchemistry) (Detection of mouse PU.1 in RENCA syngeneic tumor by IHC. Antibody: Rabbit anti-PU.1 recombinant monoclonal antibody (AAA23854 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB)

IHC (Immunohistochemistry) (Detection of human PU.1 in Non-hodgkin lymphoma by IHC. Antibody: Rabbit anti-PU.1 recombinant monoclonal antibody (AAA23854 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB)

IHC (Immunohistochemistry) (Detection of mouse PU.1 in bone marrow by IHC. Antibody: Rabbit anti-PU.1 recombinant monoclonal antibody (AAA23854 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB)

ICC (Immunocytochemistry) (Detection of mouse PU.1 in RAW 246.7 cells by ICC. Antibody: Rabbit anti-PU.1 recombinant monoclonal antibody (AAA23854 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB)

ICC (Immunocytochemistry) (Detection of human PU.1 in THP-1 cells by ICC. Antibody: Rabbit anti-PU.1 recombinant monoclonal antibody (AAA23854 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB)

FCM (Flow Cytometry) (Detection of human PU.1 (shaded) in K562 cells by flow cytometry. Antibody: Rabbit anti-PU.1 recombinant monoclonal antibody (AAA23854) or isotype control (unshaded). Secondary: DyLight 650-conjugated goat anti-rabbit IgG .)

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PU.1, Monoclonal Recombinant Antibody (Cat# AAA23854)

Full Name

Rabbit anti-PU.1 Recombinant Monoclonal Antibody [BLR179J]

Gene Names

SPI1; OF; PU.1; SFPI1; SPI-1; SPI-A

Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry

Purity

Purified

Pricing

$295/0.01 mL $680/0.1 mL $980/5x0.01 mL $1,300/2x0.1 mL $1,890/3x0.1 mL $2,440/4x0.1 mL $2,755/5x0.1 mL

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IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using T mouse mAb with DAB staining.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded cervical cancer tissues using T mouse mAb with DAB staining.)

FCM (Flow Cytometry) (Flow cytometric analysis of HeLa cells using T mouse mAb (green) and negative control (red).)

WB (Western Blot) (Western blot analysis using T mouse mAb against Raji (1), and Jurkat (2) cell lysate.)

WB (Western Blot) (Western blot analysis using T mAb against HEK293 (1) and T (AA)

WB (Western Blot) (Western blot analysis using T mAb against human T recombinant protein. (Expected MW is 31.2 kDa))

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T, Monoclonal Antibody (Cat# AAA14126)

Full Name

Anti-T Mouse mAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Pricing

$565/0.1 mL $1,070/2x0.1 mL $1,545/3x0.1 mL $1,900/5x0.1 mL $1,995/4x0.1 mL

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Application Data (Detection limit for recombinant GST tagged PCAF is approximately 3ng/ml as a capture antibody.)

WB (Western Blot) (PCAF monoclonal antibody (M06), clone 5E5. Western Blot analysis of PCAF expression in human uterus myoma.)

WB (Western Blot) (PCAF monoclonal antibody (M06), clone 5E5. Western Blot analysis of PCAF expression in C32 (Cat # L002V1).)

WB (Western Blot) (PCAF monoclonal antibody (M06), clone 5E5. Western Blot analysis of PCAF expression in A-431 (Cat # L015V1).)

WB (Western Blot) (PCAF monoclonal antibody (M06), clone 5E5 Western Blot analysis of PCAF expression in Hela S3 NE (Cat # L013V3).)

WB (Western Blot) (PCAF monoclonal antibody (M06), clone 5E5. Western Blot analysis of PCAF expression in human thyroid (diffuse hyperplasia).)

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PCAF, Monoclonal Antibody (Cat# AAA26476)

Full Name

PCAF (K(Lysine) Acetyltransferase 2B, CAF, P, P/CAF, PCAF) (HRP)

Gene Names

KAT2B; CAF; PCAF; P/CAF

Applications

Immunofluorescence, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Histone H3 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining Histone H3 in RH-35 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Histone H3 antibody. Counter stained with hematoxylin.)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Histone H3 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-Histone H3 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Histone H3 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Histone H3 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Histone H3 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Histone H3 on different lysates using anti-Histone H3 antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: NIH/3T3 Lane 3: MCF-7)

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Histone H3, Monoclonal Antibody (Cat# AAA30250)

Full Name

Histone H3 Antibody

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged NFKB1 is approximately 3ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NFKB1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to NFKB1 on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (NFKB1 monoclonal antibody (M03), clone 3F6 Western Blot analysis of NFKB1 expression in Hela S3 NE (Cat # L013V3).)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to NFKB1 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to NFKB1 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 3 ug/ml])

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NFKB1, Monoclonal Antibody (Cat# AAA26432)

Full Name

NFKB1 (Nuclear Factor of kappa Light Polypeptide Gene Enhancer in B-Cells 1, DKFZp686C01211, EBP-1, KBF1, MGC54151, NF-kappa-B, NFKB-p105, NFKB-p50, p105, p50) (HRP)

Gene Names

NFKB1; p50; KBF1; p105; EBP-1; NF-kB; CVID12; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B1

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Staining of mouse spleen cells with Rat anti Mouse CD45 (Ly-5))

Application Data (Staining of mouse spleen cells with Rat anti Mouse CD45 (Ly-5):RPE)

Application Data (Staining of mouse spleen with Rat anti Mouse CD45)

Application Data (Staining of mouse spleen with Rat anti Mouse CD45: RPE)

Application Data (Staining of mouse splenocytes with Rat anti Mouse CD45: FITC)

Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD45 (Ly-5):Alexa Fluor 647)

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CD45, Monoclonal Antibody (Cat# AAA12021)

Full Name

RAT ANTI MOUSE CD45:RPE

Gene Names

Ptprc; loc; B220; Cd45; L-CA; Ly-5; T200; CD45R; Lyt-4

Applications

Flow Cytometry

Pricing

$625/100 Tests $1,190/2x100 Tests $1,720/3x100 Tests $2,225/4x100 Tests $2,490/5x100 Tests

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ELISA (Titration curve analysis of PD-1 mAbs to detect recombinant PD-1 in ELISA abs at decreasing concentrations.)

FCM (Flow Cytometry) (Flow cytometry analysis of PD-1 overexpressing 293 cells using at 1 μg/ml. Blue: untransfected cells, Yellow: PD-1 transfected cells.)

WB (Western Blot) (Western blot analysis of PD-1 in overexpressing 293 cells using antibody at 1, 0.5, and 0.25 μg/ml, respectively.)

IF (Immunofluorescence) (Immunofluorescence of PD-1 in transfected 293 cells with PD-1 antibody at 5 μg/mL. Lower left: Immunofluorescence in transfected 293 cells with control mouse IgG antibody at 5 μg/mL.Red: PD1 Antibody [7H6]Blue: DAPI staining)

ICC (Immunocytochemistry) (Immunocytochemistry of PD-1 in transfected 293 cells with PD-1 antibody at 5 μg/mL. Lower left: Immunocytochemistry in transfected 293 cells with control mouse IgG antibody at 5 μg/mL.)

IHC (Immunohistochemistry) (Immunohistochemistry of PD-1 in (A) human breast cancer tissue and (B) human normal breast tissue with PD-1 antibody at 5 μg/mL.)

IHC (Immunohistochemistry) (Immunohistochemistry of PD-1 in (A) human tonsil tissue, (B) human lymph node tissue, and (C) human spleen tissue with PD-1 antibody at 5 μg/mL. (D) Immunohistochemistry in human tonsil tissue with control mouse IgG staining at 5 μg/mL.)

WB (Western Blot) (Western blot analysis of PD-1 in transfected 293 cell lysate with PD-1 antibody at 1 μg/mL.)

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PD-1, Monoclonal Antibody (Cat# AAA10980)

Full Name

PD-1 Antibody [7H6]

Gene Names

PDCD1; PD1; PD-1; CD279; SLEB2; hPD-1; hPD-l; hSLE1

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence, Flow Cytometry

Purity

Protein A purified IgG1.

Pricing

$570/0.1 mg $1,080/2x0.1 mg $1,560/3x0.1 mg $2,015/4x0.1 mg $2,295/5x0.1 mg

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IHC (Immunohistchemistry) (Figure 6. IHC analysis of DHODH using anti-DHODH antibody (AAA19712).DHODH was detected in a paraffin-embedded section of human renal carcinoma tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-DHODH Antibody (AAA19712) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 5. IHC analysis of DHODH using anti-DHODH antibody (AAA19712).DHODH was detected in a paraffin-embedded section of human placenta tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-DHODH Antibody (AAA19712) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 4. IHC analysis of DHODH using anti-DHODH antibody (AAA19712).DHODH was detected in a paraffin-embedded section of human liver cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-DHODH Antibody (AAA19712) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 3. IHC analysis of DHODH using anti-DHODH antibody (AAA19712).DHODH was detected in a paraffin-embedded section of human gastric cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-DHODH Antibody (AAA19712) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

IHC (Immunohistochemistry) (Figure 2. IHC analysis of DHODH using anti-DHODH antibody (AAA19712).DHODH was detected in a paraffin-embedded section of human breast cancer tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml mouse anti-DHODH Antibody (AAA19712) overnight at 4 degree C. Biotinylated goat anti-mouse IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) (#SA1021) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of DHODH using anti-DHODH antibody (AAA19712).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human A431 whole cell lysates,Lane 3: human HepG2 whole cell lysates,Lane 4: human MCF-7 whole cell lysates,Lane 5: rat testis tissue lysates,Lane 6: rat liver tissue lysates,Lane 7: mouse testis tissue lysates,Lane 8: mouse liver tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with mouse anti-DHODH antigen affinity purified monoclonal antibody (#AAA19712) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-mouse IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for DHODH at approximately 43 kDa. The expected band size for DHODH is at 43 kDa.)

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DHODH, Monoclonal Antibody (Cat# AAA19712)

Full Name

Anti-DHODH Antibody Picoband (monoclonal, 2G7)

Gene Names

DHODH; URA1; POADS; DHOdehase

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Immunogen affinity purified.

Pricing

$240/0.01 mg $510/0.1 mg $510/0.1 mg (Carrier Free) $730/0.1 mg (Biotin) $730/0.1 mg (Cy3) $730/0.1 mg (Dylight488) $730/0.1 mg (Dylight550) $730/0.1 mg (Dylight594) $730/0.1 mg (FITC) $730/0.1 mg (HRP) $835/0.1 mg (APC) $835/0.1 mg (iFluor647) $835/0.1 mg (PE) $1,600/2x0.1 mg (APC) $2,030/5x0.1 mg $2,315/3x0.1 mg (APC) $2,995/4x0.1 mg (APC)

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Application Data

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SDS-PAGE (SW480 whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-CK8(ABT534) antibody. The HRP-conjugated Goat anti-M)

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Cytokeratin 8, Monoclonal Antibody (Cat# AAA30888)

Full Name

Cytokeratin 8 (ABT534) Antibody

Gene Names

KRT8; K8; KO; CK8; CK-8; CYK8; K2C8; CARD2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot, Immunofluorescence

Purity

Protein G

Pricing

$240/0.02 mL $315/0.05 mL $375/0.1 mL $600/0.2 mL $1,140/2x0.2 mL $1,650/3x0.2 mL $2,135/4x0.2 mL $2,350/5x0.2 mL

What are Monoclonal Antibodies?

Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.

This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.

AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.

What Are The Uses of Monoclonal Antibodies

Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.

ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.

Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.

Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.

Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.

How Monoclonal Antibodies Are Used as Medicine?

Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).

Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:

• Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.


• Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.


• Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.

Why Buy Monoclonal Antibodies From Us?

At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.

1. Validated for Versatile Applications

The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).

2. Wide Selection & Specialized Options

We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.

3. High-Quality Proteins

Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.

Frequently Asked Questions

1. Are your monoclonal antibodies validated for specific applications?

Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.

2. How do I choose the right monoclonal antibody for my application?

Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.

3. How quickly can I receive my order?

Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.