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WB (Western Blot) (Western Blot analysis of AFP expression in transfected 293T cell line by AFP monoclonal antibody. Lane 1: AFP transfected lysate (69kD). Lane 2: Non-transfected lysate.)
Application Data (Detection limit for recombinant GST tagged AFP is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of AFP transfected lysate using AFP monoclonal antibody and Protein A Magnetic Bead and immunoblotted with AFP rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to AFP on HepG2 cell. [antibody concentration 30ug/ml].)
WB (Western Blot) (AFP monoclonal antibody Western Blot analysis of AFP expression in HepG2.)
WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)
FCM (Flow Cytometry) (Flow cytometric analysis of HepG2 cells with ADIPOR1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining ADIPOR1 in LO2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining ADIPOR1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining ADIPOR1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-ADIPOR1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-ADIPOR1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of ADIPOR1 on human skeletal muscle lysates using anti-ADIPOR1 antibody at 1/1, 000 dilution.)
Application Data (Immunoperoxidase staining of mouse lymph node cryosection stained with Rat antii Mouse CD8alpha antibody, clone KT15 followed by horseradish peroxidase conjugatedGoat anti Rat IgG s a detection reagent. High power)
Application Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD8 Alpha: Alexa Fluor 488)
Application Data (Staining of mouse spleen with Rat anti Mouse CD8 Alpha: FITC)
Application Data (Immunoperoxidase staining of mouse lymph node cryosection stained with Rat antii Mouse CD8alpha antibody, clone KT15 followed by horseradish peroxidase conjugatedGoat anti Rat IgG s a detection reagent. Medium power)
Application Data (Staining of mouse spleen cells with Rat anti Mouse CD8 Alpha: Biotin)
Application Data (Staining of mouse spleen with Rat anti Mouse CD8 Alpha: RPE - Alexa Fluor 647)
Application Data (Staining of mouse spleen cells with Rat anti Mouse CD8 Alpha:RPE)
Application Data (Staining of mouse spleen with Rat anti Mouse CD8 Alpha: RPE)
Application Data (Immunoperoxidase staining of mouse lymph node cryosection stained with Rat antii Mouse CD8alpha antibody, clone KT15 followed by horseradish peroxidase conjugatedGoat anti Rat IgG s a detection reagent. Low power)
Application Data (Staining of mouse spleen cells with Rat anti Mouse CD8)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with LDL Receptor antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining LDL Receptor in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining LDL Receptor in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining LDL Receptor in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-LDL Receptor antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-LDL Receptor antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung tissue using anti-LDL Receptor antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of LDL Receptor on MCF-7 lysates using anti-LDL Receptor antibody at 1/1, 000 dilution.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human tonsil using von Willebrand factor (VWF) Rabbit mAb (AAA28546) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon using von Willebrand factor (VWF) Rabbit mAb (AAA28546) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse lung using von Willebrand factor (VWF) Rabbit mAb (AAA28546) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human tonsil using von Willebrand factor (VWF) Rabbit mAb (AAA28546) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using von Willebrand factor (VWF) Rabbit mAb (AAA28546) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon using von Willebrand factor (VWF) Rabbit mAb (AAA28546) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using von Willebrand factor (VWF) Rabbit mAb (AAA28546) at dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
WB (Western Blot) (Western blot analysis of PDK2 over-expressed 293 cell line, cotransfected with PDK2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with PDK2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged PDK2 is ~0.3ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PDK2 on formalin-fixed paraffin-embedded human salivary gland. [antibody concentration 0.8ug/ml])
WB (Western Blot) (Western Blot analysis of PDK2 expression in transfected 293T cell line by PDK2 monoclonal antibody.Lane 1: PDK2 transfected lysate (46.2kD).Lane 2: Non-transfected lysate.)
WB (Western Blot) (PDK2 monoclonal antibody Western Blot analysis of PDK2 expression in U-2 OS.)
WB (Western Blot) (Western Blot detection against Immunogen (35.31kD).)
WB (Western Blot) (LETM1 monoclonal antibody Western Blot analysis of LETM1 expression in HeLa.)
WB (Western Blot) (Western Blot detection against Immunogen (37.62kD).)
Application Data (Detection limit for recombinant GST tagged LETM1 is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of LETM1 transfected lysate using LETM1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with LETM1 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to LETM1 on HeLa cell. [antibody concentration 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to LETM1 on formalin-fixed paraffin-embedded human small Intestine. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of LETM1 expression in transfected 293T cell line by LETM1 monoclonal antibody. Lane 1: LETM1 transfected lysate (83.4kD). Lane 2: Non-transfected lysate.)
FCM (Flow Cytometry) (Flow cytometric analysis of A549 cells with Syntenin antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining Syntenin in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Syntenin in SH-SY-5Y cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Syntenin antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Syntenin antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Syntenin on A549 cell using anti-Syntenin antibody at 1/2, 000 dilution.)
FCM (Flow Cytometry) (Flow Cytometry analysis of K562 cells stained with COX4 (red. 1/100 dilution). followed by FITC-conjugated goat anti-mouse IgG. Blue line histogram represents the isotype control. normal mouse IgG.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colorectal carcinoma with COX IV Mouse mAb (4D11-B3-E8.1:50 diluted).showing cytoplasm localization.A high pressure mediated antigen retrieval step was performed in citrate buffer(pH6.0).)
ICC (Immunocytochemistry) (Immunocytochemistry of HeLa cells using anti-COX IV mouse mAb diluted 1:150.)
IP (Immunoprecipitation) (Immunoprecipitation analysis of Hela cell lysates using COX IV mouse mAb.)
WB (Western Blot) (Western blot detection of COX IV in Mouse skeletal muscel and Jurkat lysates using COX IV mouse mAb (1:1000 diluted). Predicted band size: 17KDa.Observed band size: 17KDa.)
WB (Western Blot) (Western blot detection of COX IV in Goat muscle.CHO-k1.COS7.3T3.Hela.C6 and K562 cell lysates using COX IV mouse mAb (1:5000 diluted).Predicted band size:17KDa.Observed band size:17KDa.)
WB (Western Blot) (TUBB2A monoclonal antibody (M04), clone 3B2. Western Blot analysis of TUBB2A expression in NIH/3T3.)
WB (Western Blot) (TUBB2A monoclonal antibody (M04), clone 3B2. Western Blot analysis of TUBB2A expression in Jurkat.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TUBB2A on formalin-fixed paraffin-embedded human colon. [antibody concentration 1.5 ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to TUBB2A on formalin-fixed paraffin-embedded human colon. [antibody concentration 1.5 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TUBB2A on HeLa cell. [antibody concentration 10 ug/ml])
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TUBB2A on HeLa cell. [antibody concentration 10 ug/ml])
IP (Immunoprecipitation) (Immunoprecipitation analysis of 200ug extracts of K-562 cells using 3ug Lamin A/C antibody. Western blot was performed from the immunoprecipitate using Lamin A/C antibody at a dilition of 1:20000.)
IF (Immunofluorescence) (Confocal immunofluorescence analysis of HeLa cells using Lamin A/C antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence) (Immunofluorescence analysis of L929 cells using Lamin A/C antibody at dilution of 1:200. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using Lamin A/C antibody at dilution of 1:200. Blue: DAPI for nuclear staining.)
IF (Immunofluorescence) (Immunofluorescence analysis of H9C2 cells using Lamin A/C antibody at dilution of 1:200. Blue: DAPI for nuclear staining.)
IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human breast cancer using Lamin A/C antibody at dilution of 1:200 (40x lens).)
WB (Western Blot) (Western blot analysis of extracts from normal (control) and Lamin A/C knockout (KO) HeLa cells, using Lamin A/C antibody at 1:50000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using Lamin A/C antibody at 1:10000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.)
WB (Western Blot) (Western blot analysis of extracts of various cell lines, using Lamin A/C antibody at 1:50000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.)
WB (Western Blot) (Western blot analysis of extracts of HeLa cells, using Lamin A/C antibody at 1:10000-1:2560000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit (RM00020). Exposure time: 1s.)
Application Data (Detection limit for recombinant GST tagged PHB is ~0.03ng/ml as a capture antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PHB on HeLa cell. [antibody concentration 1 ~ 10ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PHB on formalin-fixed paraffin-embedded human tonsil tissue.[antibody concentration 5ug/ml])
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PHB on formalin-fixed paraffin-embedded human colon adenocarcinoma. [antibody concentration 5ug/ml])
WB (Western Blot) (PHB monoclonal antibody. Western Blot analysis of PHB expression in Hela.)
WB (Western Blot) (Western Blot detection against Immunogen (55.66kD).)
WB (Western Blot) (Western blot analysis of BIRC5 over-expressed 293 cell line, cotransfected with BIRC5 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with BIRC5 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)
Application Data (Detection limit for recombinant GST tagged BIRC5 is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of BIRC5 transfected lysate using BIRC5 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with BIRC5 rabbit polyclonal antibody.)
IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to BIRC5 on HeLa cell. [antibody concentration 10ug/ml].)
WB (Western Blot) (Western Blot analysis of BIRC5 expression in transfected 293T cell line by BIRC5 monoclonal antibody. Lane 1: BIRC5 transfected lysate (16.4kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using Annexin A2 Rabbit mAb (AAA28452) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse spleen tissue using Annexin A2 Rabbit mAb (AAA28452) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse liver tissue using Annexin A2 Rabbit mAb (AAA28452) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse colon tissue using Annexin A2 Rabbit mAb (AAA28452) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Annexin A2 Rabbit mAb (AAA28452) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using Annexin A2 Rabbit mAb (AAA28452) at a dilution of 1:800 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
WB (Western Blot) (Western blot analysis of various lysates using Annexin A2 Rabbit mAb (AAA28452) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
SDS-PAGE (Various whole cell lysates were separated by 8% SDS-PAGE, and the membrane was blotted with anti-MLH1(ABT-MLH1) antibody. The HRP-conjugated Goat anti-M)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded Colon. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Citric acid ,pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Colon. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Citric acid ,pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Colon carcinoma. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Citric acid ,pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded Colon carcinoma. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Citric acid ,pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Colon carcinoma. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Citric acid ,pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Colon carcinoma. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Citric acid ,pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Colon carcinoma. 1, Antibody was diluted at 1:200(4 degree overnight). 2, Citric acid ,pH6.0 was used for antigen retrieval. 3,Secondary antibody was diluted at 1:200(room temperature, 30min).)
IHC (Immunohistochemistry) (Human rectal carcinoma tissue was stained with Anti-MLH1 (ABT-MLH1) Antibody)
IHC (Immunohistochemistry) (Human rectal carcinoma tissue was stained with Anti-MLH1 (ABT-MLH1) Antibody)
IP (Immunoprecipitation) (Figure 6. Immunoprecipitation analysis. Cellular overexpression lysates (made from HEK293F cells transfected with DYKDDDDK tagged human BCMA full length gene) were pre-incubated with 6 different rabbit DimAb clones and negative control IgG. The immunocomplexes were further pulled down by protein A beads, fractionated, and blotted with mouse anti-DYKDDDDK monoclonal antibody.)
ELISA (Figure 5. ELISA plate was coated with recombinant BCMA-hFc fusion protein (PME100001), followed by pre-blocking with huC11D5.3 antibody (Grey bar) or rabbit control IgG (Black bar), and then different rabbit DimAbs antibodies were added to check the competitive inhibition of huC11D5.3. DM3 clone exhibits the strongest inhibition (Red bar). This data indicated that DM3 bind to the same epitope as bb2121.)
Application Data (Figure 4. Affinity ranking of different DimAb clones by titration of rabbit DimAb antibody concentration onto K562-BCMA or NCI-H929 cells. Different concentrations of various anti-BCMA DimAb clones were incubated with K562-BCMA (A) or NCI-H929 cells (B) at 4 degree C. Bound rabbit IgG was detected in flow cytometry analysis. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)
Application Data (Figure 3. Phylogenetic analysis of different Anti-BCMA DimAb clones. A) heavy chain and B) Light chain.)
FCM (Flow Cytometry) (Figure 2. A. Flow cytometry analysis with anti-BCMA (DM4) on NCI-H929 cells (Red histogram) or rabbit control antibody on NCI-H929 cells (Blue histogram). B. Flow cytometry data of serially titrated anti-BCMA (DM4) on NCI-H929 cells. The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)
FCM (Flow Cytometry) (Figure 1. A. Flow cytometry analysis with anti-BCMA (DM4) on K562-BCMA (Red histogram) (K562 cells stably transduced by human BCMA full length gene) and K562 (Negative control cell line) (Blue histogram). B. Flow cytometry data of serially titrated anti-BCMA (DM4). The Y-axis represents the mean fluorescence intensity (MFI) while the X-axis represents the concentration of IgG used.)
ELISA (2https://www.prosci-inc.com/tigit-antibody-7e5-16051.htmlSeptember 16, 2020Titration curve analysis of TIGIT mAbs to detectrecombinant TIGIT in ELISA with AAA11002 and antibodies atdecreasing concentrations.)
FCM (Flow Cytometry) (Flow cytometry analysis of TIGIT over expressing HEK293 cells using TIGIT antibody at 1 μg/ml. Blue: untransfected HEK293 cells. Yellow: TIGIT over expressing HEK293 cells.)
IHC (Immunohistochemistry) (Immunohistochemistry of TIGIT in human stomach carcinoma tissue using TIGIT Antibody and control mouse IgG (corner box) at 2 μg/ml.)
IF (Immunofluorescence) (Immunofluorescence of TIGIT in human stomach carcinoma tissue using TIGIT Antibody at 5 μg/ml.Green: TIGIT Antibody [7E5]Blue: DAPI staining)
IF (Immunofluorescence) (Immunofluorescence of TIGIT in over expressing HEK293 cells using TIGIT Antibody at 1 μg/ml.Green: TIGIT Antibody [7E5]Blue: DAPI staining)
ICC (Immunocytochemistry) (Immunocytochemistry of TIGIT in over expressing HEK293 cells using TIGIT antibody and control mouse IgG antibody (left corner box) at 1 μg/ml.)
FCM (Flow Cytometry) (Flow cytometric analysis of SW480 cells with Cytochrome P450 2D6 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
ICC (Immunocytochemistry) (ICC staining Cytochrome P450 2D6 in SW480 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Cytochrome P450 2D6 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Cytochrome P450 2D6 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Cytochrome P450 2D6 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Cytochrome P450 2D6 on HepG2 cell using anti-Cytochrome P450 2D6 antibody at 1/1, 000 dilution.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Rat kidney tissue using Histone H2B Rabbit mAb (AAA28536) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using Histone H2B Rabbit mAb (AAA28536) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse brain tissue using Histone H2B Rabbit mAb (AAA28536) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human spleen tissue using Histone H2B Rabbit mAb (AAA28536) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human liver tissue using Histone H2B Rabbit mAb (AAA28536) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using Histone H2B Rabbit mAb (AAA28536) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)
WB (Western Blot) (Western blot analysis of various lysates using Histone H2B Rabbit mAb (AAA28536) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Enhanced Kit (RM00021).Exposure time: 60s.)
WB (Western Blot) (EXOSC3 monoclonal antibody, Western Blot analysis of EXOSC3 expression in Hela NE.)
Application Data (Detection limit for recombinant GST tagged EXOSC3 is ~0.03ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of EXOSC3 transfected lysate using EXOSC3 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with EXOSC3 monoclonal antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to EXOSC3 on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3ug/ml])
WB (Western Blot) (Western Blot analysis of EXOSC3 expression in transfected 293T cell line by EXOSC3 monoclonal antibody. Lane 1: EXOSC3 transfected lysate (29.6kD). Lane 2: Non-transfected lysate.)
WB (Western Blot) (Western Blot detection against Immunogen (56.36kD).)
ELISA (Titration curve analysis of CTLA-4 mAbs to detect recombinant CTLA-4 in ELISA with antibodies at decreasing concentrations.)
IHC (Immunohistochemistry) (Immunohistochemistry of CTLA-4 in human tonsil tissue with CTLA-4 antibody at 5 μg/mL.)
IHC (Immunohistchemistry) (Immunohistochemistry of CTLA-4 in human lymph node tissue with CTLA-4 antibody at 5 μg/mL.)
IF (Immunofluorescence) (Immunofluorescence of CTLA-4 in human tonsil tissue with CTLA-4 antibody at 20 μg/mL.Red: CTLA4 Antibody [2G10]Blue: DAPI staining)
IF (Immunofluorescence) (Immunofluorescence of CTLA-4 in human lymph node tissue with CTLA-4 antibody at 20 μg/mL.Red: CTLA4 Antibody [2G10]Blue: DAPI staining)
IF (Immunofluorescence) (Immunofluorescence of CTLA-4 in transfected HEK293 cells with CTLA-4 antibody at 20 μg/mL.Red: CTLA4 Antibody [2G10]Blue: DAPI staining)
ICC (Immunocytochemistry) (Immunocytochemistry of CTLA-4 in transfected HEK293 cells with CTLA-4 antibody at 5 μg/mL. Lower left: Immunocytochemistry in transfected HEK293 cells with control mouse IgG antibody at 5 μg/mL.)
WB (Western Blot) (Western blot analysis of CTLA-4 in overexpressing HEK293 cells CTLA-4 antibody at 0.5 and 1 μg/mL.)
FCM (Flow Cytometry) (Flow cytometric analysis of 293T cells with Pumilio 1 antibody at 1/100 dilution (purple) compared with an unlabelled control (cells without incubation with primary antibody; yellow).Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining Pumilio 1 in SiHa cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Pumilio 1 in 293T cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Pumilio 1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Pumilio 1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human appendix tissue using anti-Pumilio 1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon tissue using anti-Pumilio 1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Pumilio 1 on SiHa cell using anti-Pumilio 1 antibody at 1/1, 000 dilution.)
ICC (Immunocytochemistry) (ICC staining Lysozyme in CRC cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Lysozyme antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse spleen tissue using anti-Lysozyme antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Lysozyme antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Lysozyme antibody. Counter stained with hematoxylin. The nuclear counter stain is DAPI (blue).)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-Lysozyme antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Lysozyme on different lysates using anti-Lysozyme antibody at 1/1, 000 dilution. Positive control: Lane 1: Mouse kidney Lane 2: HL-60)
IP (Immunoprecipitation) (Immunoprecipitation analysis of 300 ug extracts of HeLa cells using 3 ug KAP1/TRIM28 antibody (AAA28510). Western blot was performed from the immunoprecipitate using KAP1/TRIM28 antibody (AAA28510) at a dilution of 1:1000.)
IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse spleen using KAP1/TRIM28 Rabbit mAb (AAA28510) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse kidney using KAP1/TRIM28 Rabbit mAb (AAA28510) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse intestin using KAP1/TRIM28 Rabbit mAb (AAA28510) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Human spleen using KAP1/TRIM28 Rabbit mAb (AAA28510) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human kidney using KAP1/TRIM28 Rabbit mAb (AAA28510) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon using KAP1/TRIM28 Rabbit mAb (AAA28510) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using KAP1/TRIM28 Rabbit mAb (AAA28510) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)
IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse brain using KAP1/TRIM28 Rabbit mAb (AAA28510) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.)
WB (Western Blot) (Western blot analysis of various lysates using KAP1/TRIM28 Rabbit mAb (AAA28510) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with AIF antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining AIF in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining AIF in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-AIF antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse liver tissue using anti-AIF antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-AIF antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-AIF antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of AIF on different lysates using anti-AIF antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: SKOV-3)
ICC (Immunocytochemistry) (ICC staining Phospho-GSK3 beta (Ser 9) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Phospho-GSK3 beta (Ser 9) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded human pancreas tissue using anti-Phospho-GSK3 beta (Ser 9) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Phospho-GSK3 beta (Ser 9) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Phospho-GSK3 beta (Ser 9) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast tissue using anti-Phospho-GSK3 beta (Ser 9) antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Phospho-GSK3 beta (Ser 9) antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Phospho-GSK3 beta (Ser 9) on different lysates using anti-Phospho-GSK3 beta (Ser 9) antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: MCF-7)
WB (Western Blot) (P15RS monoclonal antibody. Western Blot analysis of P15RS expression in NIH/3T3.)
Application Data (Detection limit for recombinant GST tagged P15RS is ~0.03ng/ml as a capture antibody.)
IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to P15RS on formalin-fixed paraffin-embedded human kidney. [antibody concentration 3ug/ml].)
WB (Western Blot) (P15RS monoclonal antibody, Western Blot analysis of P15RS expression in A-431.)
WB (Western Blot) (P15RS monoclonal antibody. Western Blot analysis of P15RS expression in PC-12.)
WB (Western Blot) (Western Blot detection against Immunogen (36.56kD).)
Application Data (Proximity Ligation Analysis of protein-protein interactions between STAT1 and PDGFRB Mahlavu cells were stained with anti-STAT1 rabbit purified polyclonal (1:1200) and (1:50). Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).)
Application Data (Detection limit for recombinant GST tagged PDGFRB is ~1ng/ml as a capture antibody.)
IP (Immunoprecipitation) (Immunoprecipitation of PDGFRB transfected lysate using and Protein A Magnetic Bead, and immunoblotted with PDGFRB rabbit polyclonal antibody.)
WB (Western Blot) (Western Blot analysis of PDGFRB expression in human uterus myoma using.)
WB (Western Blot) (Western Blot analysis of PDGFRB expression in human stomach using.)
WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with p-HSF1 (S326) antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary)
ICC (Immunocytochemistry) (ICC staining p-HSF1 (S326) in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining p-HSF1 (S326) in AGS cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining p-HSF1 (S326) in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-p-HSF1 (S326) antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of p-HSF1 (S326) on different lysates using anti-p-HSF1 (S326) antibody at 1/1, 000 dilution. Positive control: Lane 1: Hela Lane 2: BT20 Lane 3: AGS)
Application Data (Immunoperoxidase staining of mouse lymph node cryosection eith Rat anti Mouse antibody clone R3-63 followed by horseradish peroxidase Goat anti Rat IgG antibody . Medium power)
Application Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD13 antibody, clone R3-63 , green in A and Rat anti Mouse CD8, clone YTS105.18 , red in B. Cis the merged image with nuclei counterstained blue using DAPI. Medium power)
Application Data (Staining of mouse peripheral blood mononuclear cells with Rat anti Mouse CD13: Alexa Fluor 488)
Application Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD13 antibody, clone R3-63 , green in A and Rat anti Mouse CD8, clone YTS105.18 , red in B. Cis the merged image with nuclei counterstained blue using DAPI. Low power)
Application Data (Immunoperoxidase staining of mouse lymph node cryosection eith Rat anti Mouse antibody clone R3-63 followed by horseradish peroxidase Goat anti Rat IgG antibody . Low power)
Application Data (Immunoperoxidase staining of mouse lymph node cryosection eith Rat anti Mouse antibody clone R3-63 followed by horseradish peroxidase Goat anti Rat IgG antibody . High power)
Application Data (Staining of mouse peripheral blood mononuclear cells with Rat anti Mouse CD13: Alexa Fluor 647)
Application Data (Immunofluorescence staining of a mouse lymph node cryosection with Rat anti Mouse CD13 antibody, clone R3-63 , green in A and Rat anti Mouse CD8, clone YTS105.18 , red in B. Cis the merged image with nuclei counterstained blue using DAPI. High power)
WB (Western Blot) (Detection of human PARP1 by western blot. Samples: Whole cell lysate (10 ug) from Jurkat, HeLa, A2058, and U-251MG cells prepared using NETN lysis buffer. Antibody: Rabbit anti-PARP1 recombinant monoclonal antibody (AAA23855 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 10 seconds. Lower Panel: Rabbit anti-COPB2 antibody .)
IHC (Immunohistochemistry) (Detection of mouse PARP1 in mouse ovary by IHC. Antibody: Rabbit anti-PARP1 recombinant monoclonal antibody (AAA23855 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB)
IHC (Immunohistochemistry) (Detection of human PARP1 in breast carcinoma by IHC. Antibody: Rabbit anti-PARP1 recombinant monoclonal antibody (AAA23855 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB)
ICC (Immunocytochemistry) (Detection of mouse PARP1 in AML12 cells by ICC. Antibody: Rabbit anti-PARP1 recombinant monoclonal antibody (AAA23855 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB)
ICC (Immunocytochemistry) (Detection of human PARP1 in HCT 116 cells by ICC. Antibody: Rabbit anti-PARP1 recombinant monoclonal antibody (AAA23855 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB)
FCM (Flow Cytometry) (Detection of human PARP1 (shaded) in Jurkat cells by flow cytometry. Antibody: Rabbit anti-PARP1 recombinant monoclonal (AAA23855) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)
Application Data (S100A8 in NIH-3T3 mouse cell line: S100A8 was detected in immersion fixed NIH-3T3 mouse embryonic fibroblast cell line using 10ug/mL AAA14670 for 3 hours at RT. Cells were stained (red) and counterstained with DAPI (blue).)
WB (Western Blot) (Western Blot analysis of mouse S100A8 of lysates of mouse leukocyte. PVDF membrane was probed with AAA14670 (2ug/ml) followed by IgG (HRP) anti-rat IgG secondary antibody. A specific band was detected for S100A8 at ~10kD (as indicated). This experiment was conducted under reducing conditions and using the buffers listed in the protocol.)
SDS-PAGE (Whole cell lysates were separated by 10% SDS-PAGE, and the membrane was blotted with anti-HMB45 (ABT255) antibody. The HRP-conjugated Goat anti-M)
FCM (Flow Cytometry) (Overlay histogram showing Hela cells stained with CSB-MA878942A1m (red line) at 1:300. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4 degree C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.)
Application Data (Overlay histogram showing A549 cells stained with CSB-MA878942A1m (red line) at 1:300. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4 degree C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.)
FCM (Flow Cytometry) (Overlay histogram showing 293 cells stained with CSB-MA878942A1m (red line) at 1:300. The cells were incubated in 1x PBS /10% normal goat serum to block non-specific protein-protein interactions followed by primary antibody for 1 h at 4 degree C. The secondary antibody used was FITC goat anti-mouse IgG(H+L) at 1/200 dilution for 1 h at 4 degree C. Isotype control antibody (green line) was used under the same conditions. Acquisition of >10,000 events was performed.)
IF (Immunofluorescence) (Immunofluorescence staining of Hela cells with CSB-MA878942A1m at 1:150, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).)
IF (Immunofluorescence) (Immunofluorescence staining of A549 cells with CSB-MA878942A1m at 1:150, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).)
IF (Immunofluorescence) (Immunofluorescence staining of 293 cells with CSB-MA878942A1m at 1:150, counter-stained with DAPI. The cells were blocked in 10% normal Goat Serum and then incubated with the primary antibody overnight at 4 degree C. The secondary antibody was Alexa Fluor 488-congugated AffiniPure Goat Anti-Mouse IgG(H+L).)
IHC (Immunohistochemistry) (IHC image of CSB-MA878942A1m diluted at 1:100 and staining in paraffin-embedded human colon cancer performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
IHC (Immunohistochemistry) (IHC image of CSB-MA878942A1m diluted at 1:100 and staining in paraffin-embedded human tonsil tissue performed on a Leica BondTM system. After dewaxing and hydration, antigen retrieval was mediated by high pressure in a citrate buffer (pH 6.0). Section was blocked with 10% normal goat serum 30min at RT. Then primary antibody (1% BSA) was incubated at 4 degree C overnight. The primary is detected by a biotinylated secondary antibody and visualized using an HRP conjugated SP system.)
WB (Western Blot) (Western BlotPositive WB detected in: A549 whole cell lysate, PC-3 whole cell lysate, HepG2 whole cell lysate, MCF-7 whole cell lysate, 293 whole cell lysate, Hela whole cell lysateAll lanes: PD-L1 antibody at 1:1000SecondaryGoat polyclonal to Mouse IgG at 1/10000 dilutionPredicted band size: 34, 21 kDaObserved band size: 55, 70 kDa)
FCM (Flow Cytometry) (Flow cytometric analysis of HUVEC cells with Flotillin 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti-rabbit IgG was used as the secondary antibody.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Flotillin 1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Flotillin 1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung cancer tissue using anti-Flotillin 1 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded rat brain tissue using anti-Flotillin 1 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Flotillin 1 on PC-3M cell lysate using anti-Flotillin 1 antibody at 1/500 dilution.)
FCM (Flow Cytometry) (Flow cytometric analysis of A431 cells with MMP9 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)
ICC (Immunocytochemistry) (ICC staining MMP9 in SHG-44 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining MMP9 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse placenta tissue using anti-MMP9 antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-MMP9 antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of MMP9 on rat spleen tissue lysate using anti-MMP9 antibody at 1/1, 000 dilution.)
FCM (Flow Cytometry) (Flow cytometric analysis of Raji cells with PTP1B antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody)
ICC (Immunocytochemistry) (ICC staining PTP1B in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining PTP1B in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining PTP1B in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-PTP1B antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of PTP1B on different lysates using anti-PTP1B antibody at 1/1, 000 dilution. Positive control: Lane 1: zebrafish Lane 2: MCF-7 Lane 3: HepG2 Lane 4: A431)
IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Ferritin Light Chain antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testes tissue using anti-Ferritin Light Chain antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Ferritin Light Chain antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human brain tissue using anti-Ferritin Light Chain antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver tissue using anti-Ferritin Light Chain antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Ferritin Light Chain on rat liver tissue lysate using anti-Ferritin Light Chain antibody at 1/1, 000 dilution.)
FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Cyclin H antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)
ICC (Immunocytochemistry) (ICC staining Cyclin H in PC-3M cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Cyclin H in MCF-7 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
ICC (Immunocytochemistry) (ICC staining Cyclin H in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human colon cancer tissue using anti-Cyclin H antibody. Counter stained with hematoxylin.)
IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Cyclin H antibody. Counter stained with hematoxylin.)
WB (Western Blot) (Western blot analysis of Cyclin H on K562 cells lysates using anti-Cyclin H antibody at 1/1, 000 dilution.)
Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Flow Cytometry
Purity
ProA affinity purified
Pricing
What are Monoclonal Antibodies?
Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.
This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.
AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.
What Are The Uses of Monoclonal Antibodies
Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.
ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.
Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.
Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.
Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.
How Monoclonal Antibodies Are Used as Medicine?
Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).
Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:
Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.
Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.
Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.
Why Buy Monoclonal Antibodies From Us?
At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.
1. Validated for Versatile Applications
The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).
2. Wide Selection & Specialized Options
We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.
3. High-Quality Proteins
Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.
Frequently Asked Questions
1. Are your monoclonal antibodies validated for specific applications?
Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.
2. How do I choose the right monoclonal antibody for my application?
Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.
3. How quickly can I receive my order?
Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.
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