Monoclonal Antibodies

Get accurate results in your research with our Monoclonal Antibodies, which are specially made to target exactly what you require for your research, and will produce consistent, reliable performance in lab tests.

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Application Data (Figure A. StarBright Blue 810 conjugated Mouse anti Human CD4 and Alexa Fluor 647 conjugated Mouse IgG2a isotype control. Figure B. StarBright Blue 810 conjugated Mouse anti Human CD4 and Alexa Fluor 647 conjugated Mouse anti Human CD3. All experiments performed on red cell lysed human blood gated on single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)

Application Data (Figure A. StarBright Violet 570 conjugated Mouse anti Human CD4 and Alexa Fluor 488 conjugated Mouse IgG2a isotype control. Figure B. StarBright Violet 570 conjugated Mouse anti Human CD4 and Alexa Fluor 488 conjugated Mouse anti Human CD3. All experiments performed on red cell lysed human blood gated on single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)

Application Data (Figure A. StarBright Violet 710 conjugated Mouse anti Human CD4 and Pacific Blue conjugated Mouse IgG2a isotype control . Figure B. StarBright Violet 710 conjugated Mouse anti Human CD4 and Pacific Blue conjugated Mouse anti Human CD3 . All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)

Application Data (Figure A. StarBright Blue 580 conjugated Mouse anti Human CD4 and Alexa Fluor 700 conjugated Mouse IgG2a isotype control . Figure B. StarBright Blue 580 conjugated Mouse anti Human CD4 and Alexa Fluor 700 conjugated Mouse anti Human CD3 . All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)

Application Data (Figure A. Alexa Fluor 488 conjugated Mouse anti Human CD4 and PE conjugated Mouse IgG2a isotype control . Figure B. Alexa Fluor 488 conjugated Mouse anti Human CD4 and PE conjugated Mouse anti Human CD3 . All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)

Application Data (Figure A. StarBright Violet 790 conjugated Mouse anti Human CD4 and FITC conjugated Mouse IgG2a isotype control . Figure B. StarBright Violet 790 conjugated Mouse anti Human CD4 and FITC conjugated Mouse anti Human CD3 . All experiments performed on red cell lysed human blood gated on live single cell lymphocytes, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer.)

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CD3, Monoclonal Antibody (Cat# AAA12296)

Full Name

MOUSE ANTI HUMAN CD3

Reactivity

Human

Applications

Flow Cytometry, Functional Assay, Immunohistochemistry

Purity

>90% by SDS PAGE. Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant

Pricing

$440/0.1 mg $840/2x0.1 mg $1,205/3x0.1 mg $1,555/4x0.1 mg $1,680/5x0.1 mg

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Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD11b:Biotin)

Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD11b:RPE)

Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD11b:Azide Free)

Application Data (Immunoperoxidase staining of human tonsil cryosection with Mouse anti Human CD11b antibody, clone ICRF44 followed by the Histar detection system . Low power)

Application Data (Immunoperoxidase staining of human spleen cryosection with Mouse anti Human CD11b antibody, clone ICRF44 followed by the Histar detection system . High power)

Application Data (Immunoperoxidase staining of human spleen cryosection with Mouse anti Human CD11b antibody, clone ICRF44 followed by the Histar detection system . Low power)

Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD11b:Alexa Fluor 647)

Application Data (Immunofluorescence staining of human tonsil cryosection with Mouse anti Human CD11b antibody, clone ICRF44 , red in A and Mousse anti Human CD21 , green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power)

Application Data (Immunofluorescence staining of human tonsil cryosection with Mouse anti Human CD11b antibody, clone ICRF44 , red in A and Mousse anti Human CD21 , green in B. C is the merged image with nuclei counterstained blue using DAPI. medium power)

Application Data (Staining of human peripheral blood granulocytes with Mouse anti Human CD11b:FITC)

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CD11b, Monoclonal Antibody (Cat# AAA12053)

Full Name

MOUSE ANTI HUMAN CD11b:RPE

Gene Names

ITGAM; CR3A; MO1A; CD11B; MAC-1; MAC1A; SLEB6

Applications

Flow Cytometry

Pricing

$625/100 Tests $1,190/2x100 Tests $1,720/3x100 Tests $2,225/4x100 Tests $2,490/5x100 Tests

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IP (Immunoprecipitation) (Immunoprecipitation of PSMA7 transfected lysate using PSMA7 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with PSMA7 rabbit polyclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PSMA7 on HeLa cell. [antibody concentration 1 ~ 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PSMA7 on formalin-fixed paraffin-embedded human colon tissue. [antibody concentration 5ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PSMA7 on formalin-fixed paraffin-embedded human lung, adenosqumous cell carcinoma. [antibody concentration 5ug/ml])

WB (Western Blot) (Western Blot analysis of PSMA7 expression in transfected 293T cell line by PSMA7 monoclonal antibody. Lane 1: PSMA7 transfected lysate (Predicted MW: 27.9kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (PSMA7 monoclonal antibody Western Blot analysis of PSMA7 expression in HeLa.)

WB (Western Blot) (PSMA7 monoclonal antibody. Western Blot analysis of PSMA7 expression in human omentum, serous carcinoma.)

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PSMA7, Monoclonal Antibody (Cat# AAA24329)

Full Name

PSMA7 (Proteasome Subunit alpha Type-7, Proteasome Subunit RC6-1, Proteasome Subunit XAPC7, HSPC, MGC3755) (AP)

Gene Names

PSMA7; C6; HSPC; RC6-1; XAPC7; HEL-S-276

Reactivity

Human

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western Blot analysis of B2M expression in transfected 293T cell line by B2M monoclonal antibody. Lane 1: B2M transfected lysate (13.7kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (B2M monoclonal antibody, Western Blot analysis of B2M expression in U-2 OS.)

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between CALR and B2M. HeLa cells were stained with CALR rabbit purified polyclonal 1:1200 and B2M mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Application Data (Detection limit for recombinant GST tagged B2M is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to B2M on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to B2M on formalin-fixed paraffin-embedded human esophagus. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western blot analysis of B2M over-expressed 293 cell line, cotransfected with B2M Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with B2M monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

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b2-Microglobulin, Monoclonal Antibody (Cat# AAA25163)

Full Name

b2-Microglobulin (B2M, Beta-2-microglobulin) (FITC)

Gene Names

B2M; IMD43

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (AKT1 monoclonal antibody (M08), clone 1D4. Western Blot analysis of AKT1 expression in Raw 264.7.)

WB (Western Blot) (AKT1 monoclonal antibody (M08), clone 1D4. Western Blot analysis of AKT1 expression in NIH/3T3.)

Application Data (Detection limit for recombinant GST tagged AKT1 is approximately 1ng/ml as a capture antibody.)

WB (Western Blot) (AKT1 monoclonal antibody (M08), clone 1D4 Western Blot analysis of AKT1 expression in Jurkat.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to AKT1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to AKT1 on HeLa cell. [antibody concentration 10 ug/ml])

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AKT1, Monoclonal Antibody (Cat# AAA25918)

Full Name

AKT1 (v-akt murine Thymoma Viral Oncogene Homolog 1, AKT, MGC99656, PKB, PKB-ALPHA, PRKBA, RAC, RAC-ALPHA) (AP)

Gene Names

AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Applications

Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Mouse-liver tissue. 1, PCNA Monoclonal Antibody was diluted at 1:200(4C,overnight). 2, Sodium citrate pH 6.0 wasused for antibody retrieval(>98C,20min). 3,Secondaryantibody was diluted at 1:200(room tempeRature, 30min).Negative control was used by secondary antibody only.)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded Rat-testis tissue. 1, PCNA Monoclonal Antibody was diluted at 1:200 (4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98C,20min). 3,Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, PCNA Monoclonal Antibody was diluted at 1:200 (4C,overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98C,20min). 3,Secondary antibody was diluted at 1:200 (room tempeRature, 30min).Negative control was used by secondary antibody only.)

IF (Immunofluorescence) (Immunofluorescence analysis of Rat-testis tissue. 1,PCNA Monoclonal Antibody (red) was diluted at 1:200 (4C,overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A: Target. Picture B: DAPI. PictureC: merge of A+B.)

IF (Immunofluorescence) (Immunofluorescence analysis of Human-lung-cancer tissue. 1,PCNA Monoclonal Antibody(red) was diluted at 1:200 (4C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI(blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B.)

IHC (Immunohistochemistry) (IHC staining of paraffin- embedded Human breast cancer tissue with PCNA mouse mAb (12D10) diluted at 1:200.)

WB (Western Blot) (Western blot analysis of Hela (lane 1), Rat brain (lane 2), NIH 3T3 (lane 3), 293T (lane 4), using diluted at 1:5000.)

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PCNA, Monoclonal Antibody (Cat# AAA29641)

Full Name

PCNA Mouse Monoclonal Antibody

Gene Names

PCNA; ATLD2

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry

Purity

Affinity purification using immunogen.

Pricing

$365/0.1 mL $680/2x0.1 mL $980/3x0.1 mL $1,260/4x0.1 mL $1,355/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of MCF7 cells using Cytokeratin 19 (KRT19) Rabbit mAb (AAA28472) at dilution of 1:20 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.)

IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Human placenta tissue using Cytokeratin 19 (CK19) Rabbit mAb (AAA28472) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human pancreas tissue using Cytokeratin 19 (CK19) Rabbit mAb (AAA28472) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human lung squamous carcinoma tissue using Cytokeratin 19 (CK19) Rabbit mAb (AAA28472) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)

IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Human liver tissue using Cytokeratin 19 (CK19) Rabbit mAb (AAA28472) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human fallopian tube tissue using Cytokeratin 19 (CK19) Rabbit mAb (AAA28472) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma tissue using Cytokeratin 19 (CK19) Rabbit mAb (AAA28472) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human cervix cancer tissue using Cytokeratin 19 (CK19) Rabbit mAb (AAA28472) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human breast cancer tissue using Cytokeratin 19 (CK19) Rabbit mAb (AAA28472) at a dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.)

WB (Western Blot) (Western blot analysis of various lysates, using Cytokeratin 19 (CK19) Rabbit mAb (AAA28472) at 1:500 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Negative control (NC): HeLaExposure time: 0.5s.)

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Cytokeratin 19 (CK19), Monoclonal Antibody (Cat# AAA28472)

Full Name

Cytokeratin 19 (CK19) Rabbit mAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Affinity purification

Pricing

$300/0.02 mL $500/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,780/4x0.1 mL $1,960/5x0.1 mL

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 300 ug extracts from HeLa cells using 3 ug KLF4 Rabbit mAb (AAA28462). Western blot was performed from the immunoprecipitate using KLF4 Rabbit mAb (AAA28462) at a dilution of 1:1000.)

ICC (Immunocytochemistry) (Confocal imaging of U-2 OS cells using KLF4 Rabbit mAb (AAA28462,dilution 1:100)(Red). The cells were counterstained with ?-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.)

IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse kidney using KLF4 Rabbit mAb (AAA28462) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse brain using KLF4 Rabbit mAb (AAA28462) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human liver cancer using KLF4 Rabbit mAb (AAA28462) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon using KLF4 Rabbit mAb (AAA28462) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

WB (Western Blot) (Western blot analysis of lysates from Mouse lung, using KLF4 Rabbit mAb (AAA28462) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 3min.)

WB (Western Blot) (Western blot analysis of various lysates using KLF4 Rabbit mAb (AAA28462) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 30s.)

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KLF4, Monoclonal Antibody (Cat# AAA28462)

Full Name

KLF4 Rabbit mAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation

Purity

Affinity purification

Pricing

$300/0.02 mL $500/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,780/4x0.1 mL $1,960/5x0.1 mL

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ICC (Immunocytochemistry) (ICC staining HBG1/2 in PC-12 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining HBG1/2 in MCF-7 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining HBG1/2 in D3 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-HBG1/2 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil tissue using anti-HBG1/2 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of HBG1/2 on human placenta (1) and human brain (2) tissue lysates using anti-HBG1/2 antibody at 1/500 dilution.)

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Hemoglobin Subunit gamma 1 and 2, Monoclonal Antibody (Cat# AAA30321)

Full Name

Hemoglobin Subunit gamma 1 and 2 Antibody

Gene Names

HBG1; HBGA; HBGR; HBG-T2; HSGGL1; PRO2979

Reactivity

Human

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using CASP3 antibody.)

IF (Immunofluorescence) (Immunofluorescence analysis of PC-3M cells using CASP3 antibody.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using CASP3 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human tonsil using CASP3 antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human spleen using CASP3 antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of Jurkat cells, using CASP3 antibody.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.)

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CASP3, Monoclonal Antibody (Cat# AAA28234)

Full Name

CASP3 Monoclonal Antibody

Gene Names

CASP3; CPP32; SCA-1; CPP32B

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Purity

Affinity Purification

Pricing

$300/0.02 mL $500/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,780/4x0.1 mL $1,960/5x0.1 mL

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Application Data

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CD274, Monoclonal Antibody (Cat# AAA26716)

Full Name

CD274 (CD274 Antigen, B7 Homolog 1, B7H1, B7-H1, B7-H, MGC142294, MGC142296, OTTHUMP00000021029, Programmed Cell Death 1 Ligand 1, PDCD1 Ligand 1, PDCD1L1, PDCD1LG1, Programmed Death Ligand 1, PDL1, PD-L1, RGD1566211) (AP)

Gene Names

CD274; B7-H; B7H1; PDL1; PD-L1; PDCD1L1; PDCD1LG1

Reactivity

Human

Applications

ELISA

Purity

Purified by protein G affinity chromatography from tissue culture supernatant.

Pricing

$830/0.1 mL $1,590/2x0.1 mL $2,305/3x0.1 mL $2,980/4x0.1 mL $3,425/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of C2C12 cells with Desmin antibody at 1/50 dilution (blue) compared with an unlabelled control (cells without incubation with primary antibody; red). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the secondary antibody.)

ICC (Immunocytochemistry) (ICC staining Desmin in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse heart tissue using anti-Desmin antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue using anti-Desmin antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse bladder tissue using anti-Desmin antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human uterus tissue using anti-Desmin antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Desmin on different lysates using anti-Desmin antibody at 1/1, 000 dilution. Positive control: Lane 1: Human skeletal muscle Lane 2: Mouse heart)

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Desmin, Monoclonal Antibody (Cat# AAA30041)

Full Name

Desmin Antibody

Gene Names

DES; CSM1; CSM2

Reactivity

Human, Mouse, Rat, Zebrafish

Applications

Western Blot, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of MCF-7 cells with EFTUD2 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without ncubation with primary antibody; black). Goat anti mouse IgG (FITC) was used as the secondary antibody.)

ICC (Immunocytochemistry) (Immunocytochemical staining of SW480 cells using anti-EFTUD2 Mouse mAb.)

ICC (Immunocytochemistry) (Immunocytochemical staining of SH-SY-5Y cells using anti-EFTUD2 Mouse mAb)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin- embedded mouse prostate tissue using anti-EFTUD2 Mouse mAb.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin- embedded mouse colon tissue using anti-EFTUD2 Mouse mAb.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin- embedded human colon cancer tissue using anti-EFTUD2 Mouse mAb.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin- embedded human lung cancer tissue using anti-EFTUD2 Mouse mAb.)

WB (Western Blot) (Western blot analysis on different cell lysates using anti-EFTUD2 Mouse mAb. Positive control: Lane 1: Jurkat Lane 2: NIH/3T3)

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Eftud2, Monoclonal Antibody (Cat# AAA29864)

Full Name

Eftud2 Antibody

Gene Names

Eftud2; 116kDa; Snrp116; U5-116kD

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,015/5x0.1 mL

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Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD11 antibody, clone ED8 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. High power)

Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD11 antibody, clone ED8 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Low power)

Application Data (Published customer image: TLR4 regulates microglial activation. Primary microglial cells were transfected with TLR4 siRNA (100 nM) or control (siControl) for 48 hours and then stimulated with lipopolysaccharide (LPS) (10 ng/ml) for 24 hours. (a) Suppression of TLR4 expression by TLR4 siRNA. (b) Quantification of IL-6 and TNF-a production in media, **P)

Application Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD11b antibody, clone ED8 , red in A and CD4 , green in B. C is the merged image with nuclei counterstained blue using DAPI. High power)

Application Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD11 antibody, clone ED8 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 as a detection reagent. Medium power)

Application Data (Published customer image: Lithium inhibits lipopolysaccharide (LPS)-induced microglial activation. Primary microglial cells were pretreated with LiCl at 0.3, 1 and 3 mM for 30 minutes and then stimulated with LPS at 10 ng/ml for 24 hours. (a) and (b) For flow cytometric analysis, the cells were incubated with PE-conjugated ED8 antibody at 37 degree C for 1 hour. **P)

Application Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD11b antibody, clone ED8 , red in A and CD4 , green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power)

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CD11b, Monoclonal Antibody (Cat# AAA11887)

Full Name

MOUSE ANTI RAT CD11b:FITC

Applications

Flow Cytometry

Pricing

$555/100 Tests $1,045/2x100 Tests $1,510/3x100 Tests $1,955/4x100 Tests $2,165/5x100 Tests

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Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)

Application Data (Published customer image: Post-injury 7,8-dihydroxyflavone treatment increased brain BDNF levels and promoted CREB activation. (A) Bar graphs demonstrating brain derived neurotrophic factor (BDNF) protein concentrations measured by enzyme-linked immunosorbent assay (ELISA) in sham-injured, vehicle-treated, and 20 mg/kg 7,8-dihydroxyflavone (DHF 20)-treated mice at 4 days post-TBI. DHF 20 significantly increased BDNF protein levels in the ipsilateral hemisphere at 4 days post-injury. (B) Bar graphs demonstrating BDNF mRNA expression measured by real -time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) at 1 and 4 days post-injury. DHF 20 significantly increased BDNF mRNA levels in the ipsilateral hemisphere at 4 days post-injury. (C) Western blot analysis of the phospho-CREB level in the ipsilateral hemisphere of sham-injured, vehicle-treated, and DHF 20-treated mice at 1 h, 1 day, and 4 days post-injury. DHF 20 enhanced CREB phosphorylation at 4 days post-injury. (D) Identification of phospho-CREB-positive cells 4 day post-injury in the peri-contusional margin by double immunofluorescence staining. Phospho-CREB is shown in red, and NeuN (neurons), GFAP (astrocytes), or F4/80 (microglia) is shown in green. Co-localization of phospho-CREB with NeuN is shown by yellow labeling. Sections were stained with DAPI (blue) to show all nuclei. Values are mean +/- SEM; *P)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: Pacific Blue)

Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen:Biotin)

Application Data (Frozen mouse spleen stained with A: Rat anti Mouse F4/80 followed by Goat anti Rat IgG:HRP or B: Rat anti Mouse F4/80 preincubated with 2 molar excess of Human anti Idiotypic (HCA154) followed by Goat anti Rat IgG:HRP)

Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen:Low Endotoxin)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: RPE - Alexa Fluor 750)

Application Data (Staining of mouse peritoneal macrophages cells with Rat anti Mouse F4/80 antigen:APC)

Application Data (Immunofluorescence image of mouse small intestine stained with Rat anti Mouse F4/80 , red and Rat anti Mouse CD45 , green; nuclei are stained with DAPI, blue. The image higlights macrophages within the lamina propria)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen:FITC)

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F4/80, Monoclonal Antibody (Cat# AAA12161)

Full Name

RAT ANTI MOUSE F4/80:APC

Gene Names

Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7

Applications

Flow Cytometry

Pricing

$685/100 Tests $1,310/2x100 Tests $1,905/3x100 Tests $2,460/4x100 Tests $2,770/5x100 Tests

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Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen Biotin)

Application Data (Published customer image: Post-injury 7,8-dihydroxyflavone treatment increased brain BDNF levels and promoted CREB activation. (A) Bar graphs demonstrating brain derived neurotrophic factor (BDNF) protein concentrations measured by enzyme-linked immunosorbent assay (ELISA) in sham-injured, vehicle-treated, and 20 mg/kg 7,8-dihydroxyflavone (DHF 20)-treated mice at 4 days post-TBI. DHF 20 significantly increased BDNF protein levels in the ipsilateral hemisphere at 4 days post-injury. (B) Bar graphs demonstrating BDNF mRNA expression measured by real -time quantitative reverse transcriptase polymerase chain reaction (RT-PCR) at 1 and 4 days post-injury. DHF 20 significantly increased BDNF mRNA levels in the ipsilateral hemisphere at 4 days post-injury. (C) Western blot analysis of the phospho-CREB level in the ipsilateral hemisphere of sham-injured, vehicle-treated, and DHF 20-treated mice at 1 h, 1 day, and 4 days post-injury. DHF 20 enhanced CREB phosphorylation at 4 days post-injury. (D) Identification of phospho-CREB-positive cells 4 day post-injury in the peri-contusional margin by double immunofluorescence staining. Phospho-CREB is shown in red, and NeuN (neurons), GFAP (astrocytes), or F4/80 (microglia) is shown in green. Co-localization of phospho-CREB with NeuN is shown by yellow labeling. Sections were stained with DAPI (blue) to show all nuclei. Values are mean +/- SEM; *P)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: Pacific Blue)

Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen:Biotin)

Application Data (Frozen mouse spleen stained with A: Rat anti Mouse F4/80 followed by Goat anti Rat IgG:HRP or B: Rat anti Mouse F4/80 preincubated with 2 molar excess of Human anti Idiotypic (HCA154) followed by Goat anti Rat IgG:HRP)

Application Data (Staining of J774 cells with Rat anti Mouse F4/80 antigen:Low Endotoxin)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen: RPE - Alexa Fluor 750)

Application Data (Staining of mouse peritoneal macrophages cells with Rat anti Mouse F4/80 antigen:APC)

Application Data (Immunofluorescence image of mouse small intestine stained with Rat anti Mouse F4/80 , red and Rat anti Mouse CD45 , green; nuclei are stained with DAPI, blue. The image higlights macrophages within the lamina propria)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse F4/80 antigen:FITC)

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F4/80, Monoclonal Antibody (Cat# AAA12172)

Full Name

RAT ANTI MOUSE F4/80:RPE

Gene Names

Emr1; Ly71; F4/80; Gpf480; TM7LN3; DD7A5-7; EGF-TM7

Applications

Flow Cytometry, Immunofluorescence

Pricing

$315/25 Tests $585/2x25 Tests $835/3x25 Tests $1,075/4x25 Tests $1,090/5x25 Tests

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IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded rectum cancer tissues using CAMK4 mouse mAb with DAB staining.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded ovarian cancer tissues using CAMK4 mouse mAb with DAB staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of HepG2 cells using CAMK4 mouse mAb (green). Blue)

WB (Western Blot) (Western blot analysis using CAMK4 mouse mAb against Jurkat (1), SK-N-SH (2), Raji (3), and HeLa (4) cell lysate.)

WB (Western Blot) (Western blot analysis using CAMK4 mAb against HEK293 (1) and CAMK4 (AA)

WB (Western Blot) (Western blot analysis using CAMK4 mAb against human CAMK4 recombinant protein. (Expected MW is 54.8 kDa))

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CAMK4, Monoclonal Antibody (Cat# AAA14128)

Full Name

Anti-CAMK4 Mouse mAb

Gene Names

CAMK4; caMK; CaMK IV; CaMK-GR

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Flow Cytometry

Pricing

$565/0.1 mL $1,070/2x0.1 mL $1,545/3x0.1 mL $1,900/5x0.1 mL $1,995/4x0.1 mL

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WB (Western Blot) (Detection of human T-bet/TBX21 by western blot. Samples: Whole cell lysate (50 ug) from MCF-7, HEK293T, MJ/G11, HeLa, Jurkat, NK-92, Hep-G2, and U2OS cells prepared using NETN lysis buffer. Antibody: Rabbit anti-T-bet/TBX21 recombinant monoclonal antibody (AAA23837 lot 1) used at 1:1000. Secondary: HRP-conjugated goat anti-rabbit IgG . Detection: Chemiluminescence with an exposure time of 30 seconds. Lower Panel: Rabbit anti-Actin recombinant monoclonal .)

IP (Immunoprecipitation) (Detection of human T-bet/TBX21 by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from MJ/G11 cells prepared using NETN lysis buffer. Antibodies: Rabbit anti-T-bet/TBX21 recombinant monoclonal antibody (AAA23837 lot 1) used for IP at 6 ul per reaction. T-bet/TBX21 was also immunoprecipitated by rabbit anti-T-bet/TBX21 antibody (BL-1505B-4G6). For blotting immunoprecipitated T-bet/TBX21, AAA23837 was used at 1:1000. Detection: Chemiluminescence with an exposure time of 3 seconds.)

IHC (Immunohistochemistry) (Detection of human T-bet/TBX21 in FFPE Hodgkin's lymphoma by IHC. Antibody: Rabbit anti-T-bet/TBX21 recombinant monoclonal antibody (AAA23837 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

IHC (Immunohistochemistry) (Detection of human T-bet/TBX21 in FFPE seminoma by IHC. Antibody: Rabbit anti-T-bet/TBX21 recombinant monoclonal antibody (AAA23837 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

ICC (Immunocytochemistry) (Detection of human T-bet/TBX21 in FFPE MJ cells by ICC. Antibody: Rabbit anti-T-bet/TBX21 recombinant monoclonal antibody (AAA23837 lot 1). Secondary: HRP-conjugated goat anti-rabbit IgG . Substrate: DAB.)

FCM (Flow Cytometry) (Detection of human T-Bet/TBX21 (shaded) in MJ[G11] cells by flow cytometry. Antibody: Rabbit anti-T-Bet/TBX21 recombinant monoclonal (AAA23837 lot 1) or isotype control (unshaded). Secondary: DyLight 488-conjugated goat anti-rabbit IgG .)

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T-bet/TBX21, Monoclonal Recombinant Antibody (Cat# AAA23837)

Full Name

Rabbit anti-T-bet/TBX21 Recombinant Monoclonal Antibody [BLR110H]

Gene Names

TBX21; TBET; T-PET; T-bet; TBLYM

Reactivity

Human

Applications

Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry, Flow Cytometry

Purity

Purified

Pricing

$295/0.01 mL $680/0.1 mL $980/5x0.01 mL $1,300/2x0.1 mL $1,890/3x0.1 mL $2,440/4x0.1 mL $2,755/5x0.1 mL

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WB (Western Blot) (F3 monoclonal antibody, Western Blot analysis of F3 expression in A-431.)

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between F7 and F3. HeLa cells were stained with F7 rabbit purified polyclonal 1:1200 and F3 mouse monoclonal antibody 1:50. Signals were detected by 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex)

Application Data (Detection limit for recombinant GST tagged F3 is ~0.03ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of F3 transfected lysate using F3 monoclonal antibody and Protein A Magnetic Bead and immunoblotted with F3 rabbit polyclonal antibody.)

WB (Western Blot) (Western Blot analysis of F3 expression in transfected 293T cell line by F3 monoclonal antibody. Lane 1: F3 transfected lysate (33.1kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (37.84kD).)

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CD142, Monoclonal Antibody (Cat# AAA25636)

Full Name

CD142 (CD142 Antigen, Coagulation Factor III, F3, Tissue Factor, TF, TFA, Thromboplastin) (PE)

Gene Names

F3; TF; TFA; CD142

Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (FOXA1 monoclonal antibody (M05), clone 3C1. Western Blot analysis of FOXA1 expression in HepG2.)

WB (Western Blot) (FOXA1 monoclonal antibody (M05), clone 3C1 Western Blot analysis of FOXA1 expression in MCF-7.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to FOXA1 on HeLa cell. [antibody concentration 10 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to FOXA1 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 1.5 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to FOXA1 on formalin-fixed paraffin-embedded human prostate. [antibody concentration 1.5 ug/ml])

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FOXA1, Monoclonal Antibody (Cat# AAA25933)

Full Name

FOXA1 (Forkhead Box A1, HNF3A, MGC33105, TCF3A) (AP)

Gene Names

FOXA1; HNF3A; TCF3A

Applications

Immunohistochemistry, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: WB: 1:2000 IHC: 1:200 IF 1:200)

WB (Western Blot) (Dilution: WB: 1:2000 IHC: 1:200 IF 1:200)

IF (Immunofluorescence) (Dilution: WB: 1:2000 IHC: 1:200 IF 1:200)

IF (Immunofluorescence) (Dilution: WB: 1:2000 IHC: 1:200 IF 1:200)

IHC (Immunohistochemistry-Paraffin) (Dilution: WB: 1:2000 IHC: 1:200 IF 1:200)

IHC (Immunohistochemistry-Paraffin) (Dilution: WB: 1:2000 IHC: 1:200 IF 1:200)

WB (Western Blot) (Dilution: WB: 1:2000 IHC: 1:200 IF 1:200)

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NSE, Monoclonal Antibody (Cat# AAA28868)

Full Name

NSE Monoclonal Antibody (13E2)

Gene Names

ENO2; NSE

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence

Pricing

$510/0.1 mL $975/2x0.1 mL $1,415/3x0.1 mL $1,825/4x0.1 mL $2,070/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of A431 cells with Laminin beta 1 antibody at 1/50 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black). Alexa Fluor 488-conjugated goat anti rabbit IgG was used as the s �� ��  �� �c��% )

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse small intestine tissue using anti-Laminin beta 1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Laminin beta 1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse prostate tissue using anti-Laminin beta 1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Laminin beta 1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Laminin beta 1 antibody. Counter stained with hematoxylin.)

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Laminin beta 1, Monoclonal Antibody (Cat# AAA30303)

Full Name

Laminin beta 1 Antibody

Gene Names

LAMB1; CLM; LIS5

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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Application Data

Application Data

Application Data

Application Data

Application Data

Application Data

SDS-PAGE (Various whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-GST-Pi (ABT-GSTP1) antibody. The HRP-conjugated Goat anti-M)

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GST-Pi, Monoclonal Antibody (Cat# AAA30842)

Full Name

GST-Pi (ABT-GSTP1) Antibody

Gene Names

GSTP1; PI; DFN7; GST3; GSTP; FAEES3

Reactivity

Human

Applications

Immunohistochemistry

Purity

The antibody was affinity-purified from ascites by affinity-chromatography using specific immunogen.

Pricing

$240/0.02 mL $315/0.05 mL $375/0.1 mL $600/0.2 mL $1,140/2x0.2 mL $1,650/3x0.2 mL $2,135/4x0.2 mL $2,350/5x0.2 mL

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ICC (Immunocytochemistry) (ICC staining Integrin beta 3 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Integrin beta 3 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistchemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Integrin beta 3 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using anti-Integrin beta 3 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human liver cancer tissue using anti-Integrin beta 3 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse testis tissue using anti-Integrin beta 3 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human spleen tissue using anti-Integrin beta 3 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Integrin beta 3 on HUVEC cells lysates using anti- Integrin beta 3 antibody at 1/1, 000 dilution.)

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Integrin beta 3, Monoclonal Antibody (Cat# AAA30047)

Full Name

Integrin beta 3 Antibody

Gene Names

ITGB3; GT; CD61; GP3A; BDPLT2; GPIIIa; BDPLT16

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunocytochemistry, Immunofluorescence

Purity

ProA affinity purified

Pricing

$380/0.05 mL $525/0.1 mL $995/2x0.1 mL $1,440/3x0.1 mL $1,865/4x0.1 mL $2,070/5x0.1 mL

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ICC (Immunocytochemistry) (Immunostaining analysis in HeLa cells. HeLa cells were fixed with 4% paraformaldehyde and permeabilized with 0.1% Triton X-100 in PBS. The cells were immunostained with anti-SMAD1 mAb. [Lot No. 913C1b-1])

FCM (Flow Cytometry) (HeLa cells were fixed in 2% paraformaldehyde/PBS and then permeabilized in 90% methanol. Cells were stained with anti-SMAD1 mAb (shaded) or isotype control (unshaded) followed by Alexa Fluor(R) 488-conjugated goat anti-mouse IgG. [Lot No. 913C1b-1])

IP (Immunoprecipitation) (Immunoprecipitation: RIPA lysate of HeLa cells was incubated with anti-SMAD1 mAb. [Lot No. 913C1b-1]Predicted molecular weight: 52 kDa)

WB (Western Blot) (Detection of SMAD1 by Western blot.Samples: Whole cell lysate from human HEK293 (H, 25 ug), mouse NIH3T3 (M, 25 ug) and rat F2408 (R, 25 ug) cells. [Lot No. 913C1b-1]Predicted molecular weight: 52 kDa)

Quality Control (Arrow indicates the region of immunized recombinant protein carrying 50-200 amino acids.)

Quality Control (Western blot analysis of immunized recombinant protein, using anti-SMAD1 monoclonal antibody.)

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SMAD1, Monoclonal Antibody (Cat# AAA10611)

Full Name

Mouse monoclonal antibody Anti-Human SMAD1

Gene Names

SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1

Reactivity

Human

Applications

Dot Blot, Immunocytochemistry, Immunoprecipitation, Western Blot, Flow Cytometry

Pricing

$615/0.1 mg $1,170/2x0.1 mg $1,695/3x0.1 mg $2,190/4x0.1 mg $2,435/5x0.1 mg

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WB (Western Blot) (Western Blot analysis of IRF2 expression in transfected 293T cell line by IRF2 monoclonal antibody. Lane 1: IRF2 transfected lysate (39.4kD). Lane 2: Non-transfected lysate.)

Application Data (Detection limit for 128582 is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence in HeLa cells using 128582 (10ug/ml).)

IHC (Immunohistochemistry) (Immunoperoxidase in formalin-fixed paraffin-embedded human leiomyosarcoma usign 128582 (3ug/ml).)

WB (Western Blot) (Western Blot analysis of IRF2 expression in Hela NE using 128582.)

WB (Western Blot) (Western Blot detection against Immunogen (36.74kD).)

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IRF2, Monoclonal Antibody (Cat# AAA24840)

Full Name

IRF2 (IRF-2, Interferon Regulatory Factor 2) (Biotin)

Gene Names

IRF2; IRF-2

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged PHB is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to PHB on HeLa cell. [antibody concentration 1 ~ 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PHB on formalin-fixed paraffin-embedded human tonsil tissue.[antibody concentration 5ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to PHB on formalin-fixed paraffin-embedded human colon adenocarcinoma. [antibody concentration 5ug/ml])

WB (Western Blot) (PHB monoclonal antibody. Western Blot analysis of PHB expression in Hela.)

WB (Western Blot) (Western Blot detection against Immunogen (55.66kD).)

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PHB, Monoclonal Antibody (Cat# AAA25495)

Full Name

PHB (Prohibitin) (HRP)

Gene Names

PHB; PHB1; HEL-215; HEL-S-54e

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (DDX56 monoclonal antibody. Western Blot analysis of DDX56 expression in PC-12.)

WB (Western Blot) (DDX56 monoclonal antibody, Western Blot analysis of DDX56 expression in HeLa.)

Application Data (Detection limit for recombinant GST tagged DDX56 is ~0.1ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to DDX56 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to DDX56 on formalin-fixed paraffin-embedded human liver. [antibody concentration 3ug/ml].)

WB (Western Blot) (Western Blot analysis of DDX56 expression in transfected 293T cell line by DDX56 monoclonal antibody. Lane 1: DDX56 transfected lysate (62kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (Western Blot detection against Immunogen (36.89kD).)

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DDX56, Monoclonal Antibody (Cat# AAA25371)

Full Name

DDX56 (Probable ATP-dependent RNA Helicase DDX56, ATP-dependent 61kD Nucleolar RNA Helicase, DEAD Box Protein 21, DEAD Box Protein 56, DDX21, NOH61) (HRP)

Reactivity

Human, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (GNRHR2 monoclonal antibody, Western Blot analysis of GNRHR2 expression in HeLa.)

Application Data (Detection limit for recombinant GST tagged GNRHR2 is ~0.03ng/ml as a capture antibody.)

WB (Western Blot) (GNRHR2 monoclonal antibody. Western Blot analysis of GNRHR2 expression in NIH/3T3.)

WB (Western Blot) (GNRHR2 monoclonal antibody. Western Blot analysis of GNRHR2 expression in Raw 264.7.)

WB (Western Blot) (GNRHR2 monoclonal antibody. Western Blot analysis of GNRHR2 expression in HepG2.)

WB (Western Blot) (Western Blot detection against Immunogen (32.27kD).)

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GNRHR2, Monoclonal Antibody (Cat# AAA25114)

Full Name

GNRHR2 (Putative Gonadotropin-releasing Hormone II Receptor, GnRH II Receptor, GnRH-II-R, Type II GnRH Receptor) (FITC)

Gene Names

FZD2; Fz2; fz-2; fzE2; hFz2; OMOD2

Reactivity

Human, Mouse

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western blot analysis of IRAK1 over-expressed 293 cell line, cotransfected with IRAK1 Validated Chimera RNAi ((Lane 2) or non-transfected control (Lane 1). Blot probed with IRAK1 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged IRAK1 is ~0.03ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of IRAK1 transfected lysate using IRAK1 monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with IRAK1 rabbit polyclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to IRAK1 on HeLa cell. [antibody concentration 10ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to IRAK1 on formalin-fixed paraffin-embedded human lung. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot detection against Immunogen (43.45kD).)

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IRAK1, Monoclonal Antibody (Cat# AAA25136)

Full Name

IRAK1 (Interleukin-1 Receptor-associated Kinase 1, IRAK-1, IRAK) (FITC)

Gene Names

IRAK1; IRAK; pelle

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CAND1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to CAND1 on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (CAND1 monoclonal antibody (M05), clone 1G5. Western Blot analysis of CAND1 expression in Raw 264.7.)

WB (Western Blot) (CAND1 monoclonal antibody (M05), clone 1G5. Western Blot analysis of CAND1 expression in PC-12.)

WB (Western Blot) (CAND1 monoclonal antibody (M05), clone 1G5. Western Blot analysis of CAND1 expression in NIH/3T3.)

WB (Western Blot) (CAND1 monoclonal antibody (M05), clone 1G5. Western Blot analysis of CAND1 expression in Hela S3 NE (Cat # L013V3).)

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CAND1, Monoclonal Antibody (Cat# AAA26384)

Full Name

CAND1 (Cullin-Associated and Neddylation-dissociated 1, DKFZp434M1414, FLJ10114, FLJ10929, FLJ38691, FLJ90441, KIAA0829, TIP120, TIP120A) (FITC)

Gene Names

CAND1; TIP120; TIP120A

Applications

Immunofluorescence, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged HDAC1 is approximately 0.1ng/ml as a capture antibody.)

WB (Western Blot) (HDAC1 monoclonal antibody (M02), clone 3E1 Western Blot analysis of HDAC1 expression in Hela S3 NE.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HDAC1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to HDAC1 on HeLa cell. [antibody concentration 10 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to HDAC1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml])

Application Data (Immunoperoxidase of monoclonal antibody to HDAC1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3 ug/ml])

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HDAC1, Monoclonal Antibody (Cat# AAA26198)

Full Name

HDAC1 (Histone Deacetylase 1, DKFZp686H12203, GON-10, HD1, RPD3, RPD3L1) (Biotin)

Gene Names

HDAC1; HD1; RPD3; KDAC1; GON-10; RPD3L1

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged STAT5B is approximately 0.3ng/ml as a capture antibody.)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to STAT5B on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 1.5 ug/ml])

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to STAT5B on formalin-fixed paraffin-embedded human lymph node. [antibody concentration 1.5 ug/ml])

WB (Western Blot) (STAT5B monoclonal antibody (M03), clone 2D1 Western Blot analysis of STAT5B expression in HeLa.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to STAT5B on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to STAT5B on HeLa cell. [antibody concentration 10 ug/ml])

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STAT5B, Monoclonal Antibody (Cat# AAA26204)

Full Name

STAT5B (Signal Transducer and Activator of Transcription 5B, STAT5) (Biotin)

Gene Names

STAT5B; STAT5

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Published customer image: Leukocyte infiltration in COX-2-M/-M and COX-2+/+ mice. MPO enzymatic activity (panel A) was statistically similar in COX-2-M/-M and COX-2+/+ livers at 6 h and 24 h post-IRI. Ly-6G+ neutrophil (panel B) and granulocyte (panel C) infiltration were also comparable in COX-2-M/-M and COX-2+/+ livers after IRI. Mac-1+ (panel D) and CD68 (panel E) infiltrating macrophages were significantly reduced in COX-2-M/-M livers at 24 h post-reperfusion, but were statistically indistinguishable in COX-2-M/-M and COX-2+/+ livers at 6 h after IRI. No statistical differences in MMP-9 expression (panel F) could be demonstrated in livers of COX-2-M/-M and COX-2+/+ mice post-IRI. Representative immunostaining (panel G) of infiltrating Ly-6G+ (a,b,e,f) and Mac-1+ (c,d,g,h) leukocytes in livers of COX-2+/+ (a,c,e,g) and COX-2-M/-M (b,d,f,h) mice at 6 h (a to d) and 24 h (e to h) post IRI; (n = 5 -6/group; * indicates p)

Application Data (Published customer image: Impact of SOCS-1 deficiency on atherosclerotic plaque composition A. Atherosclerotic plaques from Socs-1-/- triple-KO (Socs-1-/-) mice showed an increased CD68 (red);iNOS (green) double-positive cell content. B. CD68 (red); CD206 (green) double-positive cells were hardly detected. C. Atherosclerotic plaques from Socs-1-/-triple-KO (Socs-1-/-) mice contained slightly more MOMA-2 (red); Ly-6C (green) double-positive cells after 4 weeks of HCD as well as D. a higher number of Ly-6G positive cells (scale bar A -C: 20 um, scale bar D:50 um). N = 5 -7 animals per group.From: Grothusen C, Schuett H, Hillmer A, Lumpe S, Grote K, et al. (2012) Role of Suppressor of Cytokine Signaling-1 In Murine Atherosclerosis. PLoS ONE 7(12): e51608.)

Application Data (Staining of mouse spleen cryosection with Rat anti Mouse CD68 followed by Goat anti Rat IgG:HRP showing staining of macrophages in the red pulp)

Application Data (Published customer image: Histology in 9V/9V/GCStg and 9V/null/GCStg mice. (A) The lung and liver sections from 9-wk old 9V/9V (row 1), 9V/9V/GCStg (row 2), 9V/null (row 3) and 9V/null/GCStg (row 4) were processed for H&E and CD68 antibody staining as indicated. Large and pale storage cells were observed in H&E stained lung and liver sections (arrows). The macrophages were indicated by anti-CD68 immunostaining (brown). Images were captured by Zeiss microscope with Spot Advance software. Scale bar was 40 um for all images. (B) The distribution and density of macrophages in 9V/null/GCStg lung and liver immunostained by anti-CD68 antibody (brown). Scale bar was 40 um for both images. (C) CD68 positive cells (CD68+) in 9V/9V/GCStg and 9V/null/GCStg lungs had significantly more CD68 stained macrophages than 9V/9V and 9V/null at 9 wks of age, respectively. The data present number of cells per image of total 5-15 images/mouse, 3 mice per genotype. Results with error bars are mean +/-S.E. The p values were from Student's t-test.From: Barnes S, Xu Y-H, Zhang W, Liou B, Setchell KDR, et al. (2014) Ubiquitous Transgene Expression of the Glucosylceramide-Synthesizing Enzyme Accelerates Glucosylceramide Accumulation and Storage Cells in a Gaucher Disease Mouse Model. PLoS ONE 9(12): e116023.)

Application Data (Published customer image: Phenotype of pancreatic macrophages. (A) Identification of macrophage subsets in pancreas of CX3CR1GFP/+ C56BL/6 mice. Pancreatic single cell suspensions were gated on FSC-A vs FSC-W and CD45+. Histograms show receptor expression profile of CX3CR1hiLYVE-1- (green line) and CX3CR1loLYVE-1+ (red line) macrophages. (B) Sorted CX3CR1loLYVE-1+ and CX3CR1hiLYVE-1- macrophages spun onto glass slides and stained with Wright's stain. 1000x magnification. (C) mRNA expression profile of chemokine receptors, chemokines and VEGFs in pancreatic macrophage subsets. mRNA levels examined by qRT-PCR in duplicate or triplicate. 6 -7 mice/group, data representative of 2 -4 separate experiments.From: Yin N, Zhang N, Lal G, Xu J, Yan M, et al. (2011) Lymphangiogenesis Is Required for Pancreatic Islet Inflammation and Diabetes. PLoS ONE 6(11): e28023.)

Application Data (Staining of mouse peritoneal macrophages with Rat anti Mouse CD68: Alexa Fluor 488 . following permeablisation with Leucoperm)

Application Data (Western Blot analysis of CD68 expression on J774 cells using Rat anti Mouse CD68 with Goat anti Rat IgG:HRP as a detection antibody)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD68 antibody, clone FA11 followed by Goat anti Rat IgG antibody . Medium power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD68 antibody, clone FA11 followed by Goat anti Rat IgG antibody . Low power)

Application Data (Staining of permeabilised mouse peritoneal macrophages with Rat anti Mouse CD68:Alexa Fluor 647 . following permeablisation with Leucoperm)

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CD68, Monoclonal Antibody (Cat# AAA12105)

Full Name

RAT ANTI MOUSE CD68:FITC

Gene Names

Cd68; Lamp4; gp110; Scard1

Applications

Flow Cytometry

Pricing

$585/0.1 mg $1,110/2x0.1 mg $1,610/3x0.1 mg $2,075/4x0.1 mg $2,295/5x0.1 mg

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WB (Western Blot) (Western blot analysis of ABL2 over-expressed 293 cell line, cotransfected with ABL2 Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with ABL2 monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Proximity Ligation Analysis (PLA) of protein-protein interactions between NCK1 and ABL2. HeLa cells were stained with NCK1 rabbit purified polyclonal 1:1200 and ABL2 mouse monoclonal antibody 1:50. Signals were detected 30 Detection Kit 613 (red), and nuclei were counterstained with DAPI (blue). Each red dot represents the detection of protein-protein interaction complex.)

Application Data (Detection limit for recombinant GST tagged ABL2 is ~0.03ng/ml as a capture antibody.)

WB (Western Blot) (Western Blot analysis of ABL2 expression in transfected 293T cell line by ABL2 monoclonal antibody. Lane 1: ABL2 transfected lysate (126.7kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (ABL2 monoclonal antibody Western Blot analysis of ABL2 expression in Hela NE.)

WB (Western Blot) (Western Blot detection against Immunogen (37kD).)

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ABL2, Monoclonal Antibody (Cat# AAA24117)

Full Name

ABL2 (Tyrosine-protein Kinase ABL2, Abelson Murine Leukemia Viral Oncogene Homolog 2, Abelson-related Gene Protein, Tyrosine Kinase ARG, ABLL, ARG) (AP)

Gene Names

ABL2; ARG; ABLL

Reactivity

Human

Applications

Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (Western Blot detection against Immunogen (54.71kD).)

WB (Western Blot) (TARDBP monoclonal antibody Western Blot analysis of TARDBP expression in A-431.)

WB (Western Blot) (Western blot analysis of TARDBP over-expressed 293 cell line, cotransfected with TARDBP Validated Chimera RNAi (Lane 2) or non-transfected control (Lane 1). Blot probed with TARDBP monoclonal antibody. GAPDH (36.1kD) used as specificity and loading control.)

Application Data (Detection limit for recombinant GST tagged TARDBP is 0.3ng/ml as a capture antibody.)

IP (Immunoprecipitation) (Immunoprecipitation of TARDBP transfected lysate using TARDBP monoclonal antibody and Protein A Magnetic Bead, and immunoblotted with TARDBP rabbit polyclonal antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to TARDBP on HeLa cell. [antibody concentration 10ug/ml])

WB (Western Blot) (Western Blot analysis of TARDBP expression in transfected 293T cell line by TARDBP monoclonal antibody. Lane 1: TARDBP transfected lysate (44.7kD). Lane 2: Non-transfected lysate.)

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TARDBP, Monoclonal Antibody (Cat# AAA24384)

Full Name

TARDBP (TAR DNA-binding Protein 43, TDP43, TDP-43, ALS10) (AP)

Gene Names

TARDBP; ALS10; TDP-43

Reactivity

Human

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged S100A6 is ~0.03ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to S100A6 on HeLa cell. [antibody concentration 10ug/ml].)

IHC (Immunohistochemistry) (Immunoperoxidase of monoclonal antibody to S100A6 on formalin-fixed paraffin-embedded human stomach carcinoma. [antibody concentration 3ug/ml])

WB (Western Blot) (Western Blot analysis of S100A6 expression in transfected 293T cell line by S100A6 monoclonal antibody. Lane 1: S100A6 transfected lysate (10.2kD). Lane 2: Non-transfected lysate.)

WB (Western Blot) (S100A6 monoclonal antibody. Western Blot analysis of S100A6 expression in HeLa.)

WB (Western Blot) (Western Blot detection against Immunogen (36.01kD).)

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S100A6, Monoclonal Antibody (Cat# AAA24647)

Full Name

S100A6 (Protein S100-A6, S100 Calcium-binding Protein A6, Calcyclin, Prolactin Receptor-associated Protein, PRA, Growth Factor-inducible Protein 2A9, MLN 4, CACY) APC

Gene Names

S100A6; 2A9; PRA; 5B10; CABP; CACY

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (Detection limit for recombinant GST tagged SMARCB1 is ~0.03ng/ml as a capture antibody.)

IHC (Immunohistchemistry) (Immunoperoxidase of monoclonal antibody to SMARCB1 on formalin-fixed paraffin-embedded human testis. [antibody concentration 3ug/ml])

WB (Western Blot) (SMARCB1 monoclonal antibody. Western Blot analysis of SMARCB1 expression in NIH/3T3.)

WB (Western Blot) (SMARCB1 monoclonal antibody. Western Blot analysis of SMARCB1 expression in Raw 264.7.)

WB (Western Blot) (SMARCB1 monoclonal antibody, Western Blot analysis of SMARCB1 expression in Hela NE.)

WB (Western Blot) (SMARCB1 monoclonal antibody, Western Blot analysis of SMARCB1 expression in PC-12.)

WB (Western Blot) (Western Blot detection against Immunogen (37.11kD).)

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SMARCB1, Monoclonal Antibody (Cat# AAA24661)

Full Name

SMARCB1 (SWI/SNF-related Matrix-associated Actin-dependent Regulator of Chromatin Subfamily B Member 1, BRG1-associated Factor 47, BAF47, Integrase Interactor 1 Protein, SNF5 Homolog, hSNF5, BAF47, INI1, SNF5L1) APC

Gene Names

SMARCB1; RDT; CSS3; INI1; SNF5; Snr1; BAF47; MRD15; RTPS1; Sfh1p; hSNFS; SNF5L1; SWNTS1; PPP1R144

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified by Protein A Affinity Chromatography.

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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WB (Western Blot) (BANF1 monoclonal antibody (M07), clone M2. Western Blot analysis of BANF1 expression in PC-12.)

WB (Western Blot) (BANF1 monoclonal antibody (M07), clone M2. Western Blot analysis of BANF1 expression in Raw 264.7.)

WB (Western Blot) (BANF1 monoclonal antibody (M07), clone M2. Western Blot analysis of BANF1 expression in Jurkat.)

Application Data (Detection limit for recombinant GST tagged BANF1 is approximately 10ng/ml as a capture antibody.)

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to BANF1 on HeLa cell. [antibody concentration 10 ug/ml])

IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to BANF1 on HeLa cell. [antibody concentration 10 ug/ml])

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BANF1, Monoclonal Antibody (Cat# AAA26177)

Full Name

BANF1 (Barrier to Autointegration Factor 1, BAF, BCRP1, D14S1460, MGC111161) (Biotin)

Gene Names

BANF1; BAF; NGPS; BCRP1; D14S1460

Applications

Immunofluorescence, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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Application Data (GM-CSF-mediated proliferation of TF-1 can be completely blocked by antibodies with an IC50 of 4.105 nM.)

Application Data (IL-3-mediated proliferation of TF-1 can be completely blocked by antibodies with an IC50 of 0.2109 nM.)

FCM (Flow Cytometry) (Human CSF2RB HEK293 cells were stained with Anti-CSF2Rb / CD131 Reference Antibody (CSL311) and negative control protein respectively, washed and then followed by PE and analyzed with FACS, EC114=0.049 ug/mL)

Application Data (Immobilized human CSF2RB His at 2 ug/mL can bind Anti-CSF2Rb / CD131 Reference Antibody (CSL311), EC50=0.0122ug/mL)

Application Data (The purity of Anti-CSF2Rb / CD131 Reference Antibody (CSL311)is more than 98.34% ,determined by SEC-HPLC.)

SDS-PAGE (Anti-CSF2Rb / CD131 Reference Antibody (CSL311) on SDS-PAGE under reducing (R) condition. The gel was stained with Coomassie Blue. The purity of the protein is greater than 95%)

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CSF2Rb/CD131, Monoclonal Recombinant Antibody (Cat# AAA29394)

Full Name

Anti-CSF2Rb/CD131 Reference Antibody (CSL311)

Gene Names

CSF2RB; CD131; IL3RB; IL5RB; SMDP5; CDw131

Reactivity

Human

Applications

Flow Cytometry, Functional Assay

Pricing

$555/0.1 mg $1,045/2x0.1 mg $1,510/3x0.1 mg $1,955/4x0.1 mg $2,145/5x0.1 mg

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IF (Immunofluorescence) (Immunofluorescence of monoclonal antibody to MAPK3 on HeLa cell. [antibody concentration 10 ug/ml])

WB (Western Blot) (Western Blot analysis of MAPK3 expression in transfected 293T cell line by MAPK3 monoclonal antibody (M05), clone 3D6.Lane 1: MAPK3 transfected lysate (43.1 KDa).Lane 2: Non-transfected lysate.)

WB (Western Blot) (MAPK3 monoclonal antibody (M05), clone 3D6. Western Blot analysis of MAPK3 expression in Raw 264.7.)

WB (Western Blot) (MAPK3 monoclonal antibody (M05), clone 3D6. Western Blot analysis of MAPK3 expression in PC-12.)

WB (Western Blot) (MAPK3 monoclonal antibody (M05), clone 3D6. Western Blot analysis of MAPK3 expression in NIH/3T3.)

WB (Western Blot) (MAPK3 monoclonal antibody (M05), clone 3D6. Western Blot analysis of MAPK3 expression in Hela S3 NE (Cat # L013V3).)

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MAPK3, Monoclonal Antibody (Cat# AAA26535)

Full Name

MAPK3 (Mitogen-Activated Protein Kinase 3, ERK1, HS44KDAP, HUMKER1A, MGC20180, P44ERK1, P44MAPK, PRKM3) (HRP)

Gene Names

MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK

Applications

Immunofluorescence, Western Blot

Purity

Purified

Pricing

$1,045/0.1 mL $2,010/2x0.1 mL $2,925/3x0.1 mL $3,785/4x0.1 mL $4,415/5x0.1 mL

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ICC (Immunocytochemistry) (Confocal imaging of NIH-3T3 cells using BRG1/SMARCA4 Rabbit mAb (AAA28507,dilution 1:100)(Red). The cells were counterstained with ?-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.)

IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Human placenta using BRG1/SMARCA4 Rabbit mAb (AAA28507) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human lung adenocarcinoma using BRG1/SMARCA4 Rabbit mAb (AAA28507) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human endometrium cancer using BRG1/SMARCA4 Rabbit mAb (AAA28507) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.)

IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon using BRG1/SMARCA4 Rabbit mAb (AAA28507) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using BRG1/SMARCA4 Rabbit mAb (AAA28507) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human cervix cancer using BRG1/SMARCA4 Rabbit mAb (AAA28507) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Tris/EDTA Buffer (pH 9.0) prior to IHC staining.)

WB (Western Blot) (Western blot analysis of lysates from Mouse brain using BRG1/SMARCA4 Rabbit mAb (AAA28507) at 1:1000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 90s.)

WB (Western Blot) (Western blot analysis of lysates from Rat brain using BRG1/SMARCA4 Rabbit mAb (AAA28507) at 1:1000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 45s.)

WB (Western Blot) (Western blot analysis of lysates from HeLa cells using BRG1/SMARCA4 Rabbit mAb (AAA28507) at 1:1000 dilution incubated overnight at 4?.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25 ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 10s.)

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BRG1/SMARCA4, Monoclonal Antibody (Cat# AAA28507)

Full Name

BRG1/SMARCA4 Rabbit mAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Purity

Affinity purification

Pricing

$300/0.02 mL $500/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,780/4x0.1 mL $1,960/5x0.1 mL

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IP (Immunoprecipitation) (Immunoprecipitation analysis of 300 ug extracts of 293T cells using 3 ug HuR/ELAVL1 antibody (AAA28517). Western blot was performed from the immunoprecipitate using HuR/ELAVL1 antibody (AAA28517) at a dilution of 1:1000.)

ICC (Immunocytochemistry) (Confocal imaging of U-2 OS cells using [KO Validated] HuR/ELAVL1 Rabbit mAb (AAA28517,dilution 1:100)(Red). The cells were counterstained with ?-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.)

ICC (Immunocytochemistry) (Confocal imaging of NIH/3T3 cells using [KO Validated] HuR/ELAVL1 Rabbit mAb (AAA28517,dilution 1:100)(Red). The cells were counterstained with ?-Tubulin Mouse mAb (AC012,dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.)

IHC (Immunohistchemistry) (Immunohistochemistry analysis of paraffin-embedded Mouse spleen using [KO Validated] HuR/ELAVL1 Rabbit mAb (AAA28517) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human tonsil using [KO Validated] HuR/ELAVL1 Rabbit mAb (AAA28517) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human kidney using [KO Validated] HuR/ELAVL1 Rabbit mAb (AAA28517) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using [KO Validated] HuR/ELAVL1 Rabbit mAb (AAA28517) at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.)

WB (Western Blot) (Western blot analysis of various lysates using [KO Validated] HuR/ELAVL1 Rabbit mAb (AAA28517) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)

WB (Western Blot) (Western blot analysis of lysates from wild type (WT) and HuR/ELAVL1 knockout (KO) 293T cells, using [KO Validated] HuR/ELAVL1 Rabbit mAb (AAA28517) at 1:1000 dilution.Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (RM00020).Exposure time: 1s.)

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HuR/ELAVL1, Monoclonal Antibody (Cat# AAA28517)

Full Name

[KO Validated] HuR/ELAVL1 Rabbit mAb

Reactivity

Human

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry, Immunoprecipitation

Purity

Affinity purification

Pricing

$300/0.02 mL $500/0.1 mL $955/2x0.1 mL $1,385/3x0.1 mL $1,780/4x0.1 mL $1,960/5x0.1 mL

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FCM (Flow Cytometry) (Flow cytometric analysis of Hela cells with Profilin 1 antibody at 1/100 dilution (red) compared with an unlabelled control (cells without incubation with primary antibody; black).)

ICC (Immunocytochemistry) (ICC staining Profilin 1 in HepG2 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Profilin 1 in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

ICC (Immunocytochemistry) (ICC staining Profilin 1 in A549 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse kidney tissue using anti-Profilin 1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded mouse colon tissue using anti-Profilin 1 antibody. Counter stained with hematoxylin.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human kidney tissue using anti-Profilin 1 antibody. Counter stained with hematoxylin.)

WB (Western Blot) (Western blot analysis of Profilin 1 on Hela cell using anti-Profilin 1 antibody at 1/1, 000 dilution.)

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Profilin 1, Monoclonal Antibody (Cat# AAA30346)

Full Name

Profilin 1 Antibody

Gene Names

PFN1; ALS18

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry, Immunohistochemistry, Flow Cytometry

Purity

ProA affinity purified

Pricing

$390/0.05 mL $495/0.1 mL $945/2x0.1 mL $1,370/3x0.1 mL $1,760/4x0.1 mL $1,965/5x0.1 mL

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Application Data (Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD4:ALEXA 405)

Application Data (Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD4: RPE-Alexa 647)

Application Data (Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD4: RPE - Alexa Fluor® 750)

Application Data (Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD4: AZIDE FREE)

Application Data (Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD4: Pacific Blue®)

Application Data (Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD4: Alexa Fluor® 488)

Application Data (Staining of human peripheral blood lymphocytes with MOUSE ANTI HUMAN CD4)

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CD4, Monoclonal Antibody (Cat# AAA26732)

Full Name

CD4 (CD4 Antigen, CD4 Molecule, CD4 Receptor, CD4mut, T Cell Surface Antigen T4/Leu3, T Cell Antigen T4, T Cell Surface Glycoprotein CD4) (Biotin)

Gene Names

CD4; CD4mut

Reactivity

Human

Applications

Flow Cytometry, Immunohistochemistry

Purity

Purified by protein A affinity chromatography from tissue culture supernatant.

Pricing

$985/0.1 mL $1,900/2x0.1 mL $2,760/3x0.1 mL $3,570/4x0.1 mL $4,155/5x0.1 mL

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Application Data (Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 followed by horseradish peroxidase Goat anti Mouse IgG2a antibody as a detection reagent. Medium power)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD3:RPE)

Application Data (Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 followed by horseradish peroxidase Goat anti Mouse IgG2a antibody as a detection reagent. Low power)

Application Data (ELISA analysis of human CD3 expression using Rat anti Human CD3ε, clone CD3-12 as a capture reagent and biotinylated Mouse anti Human CD3, clone UCHT1 as a detection reagent with recombinant human CD3 as antigen to produce the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader . A serum (green) sample is included undiluted)

Application Data (Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 followed by horseradish peroxidase Goat anti Mouse IgG2a antibody as a detection reagent. High power)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD3:Alexa Fluor 405)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD3)

Application Data (Immunoperoxidase staining of human tonsil cryosection using Mouse anti Human CD3 antibody, clone UCHT1 followed by horseradish peroxidase Goat anti Mouse IgG2a antibody as a detection reagent. High power)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD3: RPE- Alexa Fluor 750)

Application Data (Staining of human peripheral blood lymphocytes with Mouse anti Human CD3: FITC)

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CD3, Monoclonal Antibody (Cat# AAA11908)

Full Name

MOUSE ANTI HUMAN CD3:Low Endotoxin

Gene Names

CD3G; T3G; IMD17; CD3-GAMMA

Applications

Immunohistochemistry, Flow Cytometry

Pricing

$530/0.5 mg $1,005/2x0.5 mg $1,455/3x0.5 mg $1,885/4x0.5 mg $2,070/5x0.5 mg

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Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD33:RPE)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD33:Alexa Fluor 488 (AAA11928A488))

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD33)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD33:APC)

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD33:Alexa Fluor 647 (AAA11928A647))

Application Data (Staining of human peripheral blood monocytes with Mouse anti Human CD33:FITC)

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CD33, Monoclonal Antibody (Cat# AAA11928)

Full Name

MOUSE ANTI HUMAN CD33

Gene Names

CD33; p67; SIGLEC3; SIGLEC-3

Applications

Immunohistochemistry, Flow Cytometry, Immunoprecipitation, Western Blot

Pricing

$600/0.2 mg $1,140/2x0.2 mg $1,650/3x0.2 mg $2,135/4x0.2 mg $2,385/5x0.2 mg

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Application Data (Staining of mouse peripheral blood granulocytes with Rat anti Mouse CD13)

Application Data (Staining of J774 cells with Rat anti Mouse CD13:Alexa Fluor 488)

Application Data (Immunoperoxidase staining of a mouse tail cryosection with Rat anti Mouse CD13 antibody, clone ER-BMDM1 followed by horseradish peroxidase conjugated Goat anti Rat IgG Low power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD13 antibody, clone ER-BMDM1 followed by horseradish peroxidase conjugated Goat anti Rat IgG Medium power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD13 antibody, clone ER-BMDM1 followed by horseradish peroxidase conjugated Goat anti Rat IgG High power)

Application Data (Immunoperoxidase staining of a mouse tail cryosection with Rat anti Mouse CD13 antibody, clone ER-BMDM1 followed by horseradish peroxidase conjugated Goat anti Rat IgG High power)

Application Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD13 antibody, clone ER-BMDM1 followed by horseradish peroxidase conjugated Goat anti Rat IgG Low power)

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CD13, Monoclonal Antibody (Cat# AAA12145)

Full Name

RAT ANTI MOUSE CD13

Gene Names

Anpep; Apn; AP-M; AP-N; Cd13; P150

Applications

Immunohistochemistry, Flow Cytometry

Pricing

$290/0.025 mg $530/2x0.025 mg $755/3x0.025 mg $970/5x0.025 mg $975/4x0.025 mg

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Strength of Signal (Analysis of Protein Array containing more than 19,000 full-length human proteins using Vimentin (VIM) Mouse Monoclonal Antibody (VM452)Z- and S- Score: The Z-score represents the strength of a signal that a monoclonal antibody (MAb) (in combination with a fluorescently-tagged anti-IgG secondary antibody) produces when binding to a particular protein on the HuProtTM array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If targets on HuProtTM are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-score. S-score therefore represents the relative target specificity of a MAb to its intended target. A MAb is considered to specific to its intended target, if the MAb has an S-score of at least 2.5. For example, if a MAb binds to protein X with a Z-score of 43 and to protein Y with a Z-score of 14, then the S-score for the binding of that MAb to protein X is equal to 29.)

FCM (Flow Cytometry) (Flow Cytometry of human Vimentin on Jurkat Cells, Black: Cells alone; Grey: Isotype Control; Green: AF488-labeled Vimentin Mouse Monoclonal Antibody (VM452).)

SDS-PAGE (SDS-PAGE Analysis Purified Vimentin Mouse Monoclonal Antibody (VM452). Confirmation of Integrity and Purity of Antibody.)

WB (Western Blot) (Western Blot Analysis of human A375 cell lysate using Vimentin Mouse Monoclonal Antibody (VM452))

IHC (Immunohistochemistry) (Formalin-fixed, paraffin-embedded human Melanoma stained with Vimentin Mouse Monoclonal Antibody (VM452))

WB (Western Blot) (Western Blot Analysis Raji Cell Lysate, Vimentin Mouse Monoclonal Antibody (VM452))

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Vimentin, Monoclonal Antibody (Cat# AAA13810)

Full Name

Vimentin (Mesenchymal Cell Marker) Mouse Monoclonal Antibody

Gene Names

VIM; HEL113; CTRCT30

Reactivity

Human, Cow, Dog, Cat, Pig, Goat, Chicken.
Does not react with Mouse and Rat

Applications

Flow Cytometry, Immunofluorescence, Western Blot, Immunohistochemistry

Pricing

$295/0.02 mg (With BSA & Azide at 0.2mg/mL) $475/0.1 mg (With BSA & Azide at 0.2mg/mL) $475/0.1 mg (Without BSA & Azide at 1mg/mL) $905/2x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,310/3x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,690/4x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,900/5x0.1 mg (With BSA & Azide at 0.2mg/mL) $1,900/5x0.1 mg (Without BSA & Azide at 1mg/mL)

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Application Data (Figure 7. Indirect immunoperoxidase staining of human colon adenocarcinoma paraffin tissue section (RCK108; Mouse anti keratin 19). Dilution 1:50 and microwave pretreatment. Specific staining of the epithelial tumor cells. No reactivity in connective tissues.)

Application Data (Figure 6. Indirect immunoperoxidase staining of human placenta paraffin tissue section (RCK108; Mouse anti keratin 19). Dilution 1:50 and microwave pretreatment. Specific staining of the epithelial cells. No reactivity in the connective tissues.)

Application Data (Figure 5. Indirect immunoperoxidase staining of human small intestine paraffin tissue section (RCK108; Mouse anti keratin 19). Dilution 1:50 and microwave pretreatment. Specific staining of the epithelial cells. No reactivity in the connective tissues.)

IHC (Immunohistochemistry) (Figure 4. Immunohistochemistry on frozen tissue sections of human colon with (RCK108; dilution 1:500).)

IHC (Immunohistochemistry) (Figure 3. Immunohistochemistry on a frozen tissue sections of human kidney (RCK108; dilution 1:500).)

IF (Immunofluorescence) (Figure 2. immunofluorescence staining of a 9 days old zebrafish embryo.)

IHC (Immunohistochemistry) (Figure 1. immunohistochemistry on frozen section of human liver bile duct epithelium.)

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CytokeRatin 19 / KeRatin K19, Monoclonal Antibody (Cat# AAA14562)

Full Name

Mouse anti CytokeRatin 19 / KeRatin K19

Reactivity

Human, Zebrafish

Applications

Flow Cytometry, Immunocytochemistry, Immunohistochemistry, Immunohistochemistry, Western Blot

Pricing

$645/0.1 mg $1,230/2x0.1 mg $1,780/3x0.1 mg $2,305/4x0.1 mg $2,590/5x0.1 mg

What are Monoclonal Antibodies?

Monoclonal antibodies are specialized laboratory-produced proteins developed for binding to specific biological antigens or other molecular targets. Since they come from a single cell (or clone), they are especially consistent and accurate in the data they are involved in producing.

This type of antibody material has been shown to be a powerful tool in finding and subsequently destroying harmful cells in an organism, such as those found in cancers or various autoimmune diseases. This makes them excellent aids in medical testing and research, which is why they are so widely used.

AAA Biotech offers a comprehensive range of high-quality monoclonal antibodies that perform effectively in various laboratory tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry. All of the products in our catalog are thoroughly quality tested to make sure that they are reliable and will consistently perform well in your research.

What Are The Uses of Monoclonal Antibodies

Monoclonal antibodies are used in many lab tests, including (amongst others) ELISA, western blotting, immunohistochemistry, and flow cytometry.

ELISA is a test that helps detect a specific substance/analyte in a sample. It uses antibodies (often monoclonal) bound to a solid surface (such as the well of a microplate) to “capture” the substance/analyte in the sample and immobilize it so that the detection antibody component can then bind to it and produce a signal, which can then be measured.

Western blotting identifies specific proteins in a sample. The sample is first separated on a gel, and then antibodies are applied that will typically bind to the target, which will all be localized to a single band in a lane.

Immunohistochemistry helps locate specific proteins in cells or tissue samples using antibodies.

Flow cytometry looks at and sorts cells. It uses antibodies that are conjugated to reporter molecules called “fluorophores”, which, under special lights, emit light themselves, which can then be measured by a detector instrument.

How Monoclonal Antibodies Are Used as Medicine?

Please note that all of the products listed in AAA Biotech’s catalog are strictly for research-use only (RUO).

Monoclonal antibodies can also be used as therapeutic/medical treatments, particularly in the context of cancers. They are designed to find and bind to specific cells or proteins, helping the immune system recognize and attack the cancer. These treatments work in different ways, such as:

• Radioimmunotherapy attaches a small amount of radioactive molecule to the antibody, so it delivers the radiation directly to the cancer cells that the antibody is specifically binding to.


• Antibody-directed enzyme prodrug therapy uses antibodies that are specifically bound to special enzymes. These enzymes activate a harmless drug in the body and turn it into a cancer-killing drug only near the cancer cells—this helps avoid harming healthy cells.


• Immunoliposomes are tiny “bubbles” filled with medicine/drug and coated with antibodies. They carry the drug straight to the cancer cells.

Why Buy Monoclonal Antibodies From Us?

At AAA Biotech, we provide high-performance monoclonal antibodies designed to support a wide range of research needs.

1. Validated for Versatile Applications

The antibodies in our catalog are extensively validated and compatible with multiple techniques, including (but not limited to) ELISA, flow cytometry (FC), immunocytochemistry (ICC), immunofluorescence (IF), immunohistochemistry (IHC), immunoprecipitation (IP), and western blotting (WB).

2. Wide Selection & Specialized Options

We offer antibodies for common and rare species, that are available in various conjugated forms, and also in recombinant formats. Essentially, there is almost anything one might need to meet their experimental model’s requirements.

3. High-Quality Proteins

Our proteins meet high purity standards—90% or more as confirmed by SDS-PAGE. Many are available with tags like His, Flag, GST, or MBP, and we also supply native and biologically active proteins for functional studies.

Frequently Asked Questions

1. Are your monoclonal antibodies validated for specific applications?

Yes, our antibodies are tested and validated for use in methods such as ELISA, western blot, IHC, flow cytometry, and more. Refer to specific product pages or datasheets for individual product information.

2. How do I choose the right monoclonal antibody for my application?

Review the product details directly for application validation, species reactivity, and target information. You may also contact our support team at any time for help.

3. How quickly can I receive my order?

Most orders are processed and shipped within 1–3 business days, depending on product availability and your shipping location.