AAA Biotech provides a variety of high-quality recombinant and natural/native proteins that are proven to work in a wide range of experiments. Explore our products to find the active protein that best fits your needs or experimental model.
Application Data (Measured by its ability to chemoattract BaF3 mouse pro-B cells transfected with mouse CXCR6. The ED50 for this effect is 50-250 ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. 1. Immobilized recombinant human PVRL3 at 1 ug/ml (100 ul/well) can bind biotinylated Nectin-1 with a linear range of 6.4-800 ng/ml. 2. Immobilized recombinant human PVRL3 at 1 ug/ml (100 ul/well) can bind Nectin-1/ Fc chimera with a linear range of 0.156-5 ng/ml.)
Application Data (Loaded Anti-CD112 Antibody on AMC Biosensor, can bind Nectin-2 Protein, Human, Recombinant (His Tag) with an affinity constant of 34.4 nM as determined by Octet RED System.)
Application Data (Using the Octet RED System, the affinity constant (Kd) of Nectin-2 Protein, Human, Recombinant (His Tag) bound Anti-CD112 Antibody was 0.1uM.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized recombinant human CD112 at 20 ug/ml (100 ul/well) can bind biotinylated DNAM1 with a linear range of 0.078-2.5 ug/ml.)
Application Data (Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.15-0.75 ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human GPC5 at 5 ug/ml (100 ul/well) can bind human bFGF with a linear ranger of 0.156-2.5 ng/ml.)
Application Data (Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T1/2 cells. The ED50 for this effect is approximately 0.1-0.4 ug/ml in the presence of 10 ng/mL of mouse Wnt3a.)
Application Data (Measured by its ability to inhibit the biological activity of IGF-I or IGF-II on MCF-7 human breast cancer cells. The ED50 for this effect is 0.1-0.5 ug/mL in the presence of 14 ng/mL rhIGF-II.)
Application Data (Measured by its ability to inhibit Fas Ligand induced apoptosis of Jurkat human acute T cell leukemia cells. The ED50 for this effect is typically 0.5-3 ug/mL in the presence of 200 ng/mL recombinant human Fas ligand.)
Application Data (Measure by its ability to bind with human PCSK9 in a functional ELISA. Immobilized mouse PCSK9 at 10 ug/ml (100 ul/well) can bind biotinylated recombinant human LDLR. The EC50 of biotinylated human LDLR is 0.12 ug/ml.)
Application Data (Measure by its ability to bind with human PCSK9 in a functional ELISA. Immobilized human PCSK9 at 10 ug/ml (100 ul/well) can bind biotinylated recombinant human LDLR. The EC50 of biotinylated human LDLR is 0.61 ug/ml.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human EphB4 at 2 ug/ml (100 ul/well) can bind human EphrinB2 with a linear range of 1-125 ng/ml.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human MDK at 10 ug/ml (100 ul/well) can bind mouse SDC4-Fc with a linear range of 0.16-1.25 ug/ml.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human HHLA2h at 10 ug/mL (100 uL/well) can bind human TMIGD2-Fc3, the EC50 of human TMIGD2-Fc3 is 50-200 ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human His-NKG2D(78-216) at 10 ug/ml (100 ul/well) can bind RAET1L-Fc, The EC50 of RAET1L-Fc is 13-30 ng/ml.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human NCR3 at 10ug/mL (100uL/well) can bind human B7-H6 (16140-H02H), the EC50 of human B7-H6 is 20-100 ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized Human ACE2 (mFc tag) at 2 ug/mL (100 uL/well) can bind SARS-CoV-2 (2019-nCoV) Spike S1(K417N, E484K, N501Y, D614G)-His, the EC50 of SARS-CoV-2 (2019-nCoV) Spike S1(K417N, E484K, N501Y, D614G)-His is 15-90 ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized Human ACE2 (mFc tag) at 2 ug/mL (100 uL/well) can bind SARS-CoV-2 (2019-nCoV) Spike S1(HV69-70 deletion, N501Y, D614G)-His, the EC50 of SARS-CoV-2 (2019-nCoV) Spike S1(HV69-70 deletion, N501Y, D614G)-His is 8-50 ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag)(10108-H05H) at 2 ug/mL (100 uL/well) can bind SARS-CoV-2 (2019-nCoV) Spike S1-AVI & His Recombinant Protein, Biotinylated (40591-V27H-B),the EC50 of SARS-CoV-2 (2019-nCoV) Spike S1-AVI & His Recombinant Protein, Biotinylated (40591-V27H-B) is 400-800 ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag)(10108-H05H) at 2 ug/mL (100 uL/well) can bind SARS-CoV-2 (2019-nCoV) Spike RBD-His (V367F) Recombinant Protein (40592-V08H1), the EC50 of SARS-CoV-2 (2019-nCoV) Spike RBD-His (V367F) Recombinant Protein (40592-V08H1) is 20-50 ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized mouse IFNG-His at 10 ug/ml (100 ul/well) can bind mouse IFNGR1-Fc. The EC50 of IFNGR1-Fc is 58.2-135.9 ng/ml.)
Application Data (Measured in antiviral assay using L929 cells infected with vesicular stomatitisvirus (VSV). The ED50 for this effect is typically 20-80 ng/mL.)
Application Data (Measured in antiviral assays using L929 cells infected with vesicular stomatitisvirus (VSV).The ED50 for this effect is 0.05-0.3 ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized Mouse Interferon Gamma at 2 ug/ml (100 ul/well) can bind Mouse IFNGR1 hFc, the EC50 of Mouse IFNGR1 hFc is7.0-30.0 ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized Human PDGFRB at 2 ug/ml (100 ul/well) can bind Mouse PDGF-B His, the EC50 of Mouse PDGF-B His is 4-24 ng/mL.)
Application Data (Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is typically 5-40 ng/mL.)
Application Data (Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. Karey, K.P. et al. (1988) Cancer Research 48:4083. The ED50 for this effect is typically 0.05-0.2 ug/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized mouse CD27-His at 10 ug/mL (100 uL/well) can bind mouse CD70, the EC50of mouse CD70 is 10-50 ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized mouse EFNB3-His at 10 ug/mL (100 uL/well) can bind biotinylated mouse EPHB3-His, the EC50 of biotinylated mouse EPHB3-His 0.02-0.4 ug/mL.)
SDS-PAGE (Measured by its binding ability in a functional ELISA. Immobilized Mouse PDGFRA His at 2 ug/ml (100 ul/well) can bind Cynomolgus PDGF-B mFc, the EC50 of Cynomolgus PDGF-B mFc is 150-700 ng/mL.)
Application Data (Measured by its ability to inhibit recombinant rmWnt3a induced alkaline phosphatase production by C3H10T 1/2 cells. The ED50 for this effect is 0.05-0.3 ug/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized zebrafish EFNB2A-His at 10 ug/ml (100 ul/well) can bind human EphB4-Fc, The EC50 of human EphB4-Fc is 15.5-36.3 ng/ml.)
Application Data (Measured by its ability to inhibit rhBMP4-induced alkaline phosphatase production by MC3T3-E1 cells. The ED50 for this effect is typically 0.5-2.5 ug/mL in the presence of 50 ng/mL of recombinant human BMP4.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized canine EFNB2-His at 10 ug/ml (100 ul /well) can bind human EphB4-Fc, The EC50 of human EphB4-Fc is 9-20 ng/ml.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized canine NRG1 at 10 ug/ml (100 ul/well) can bind human ErbB4-Fc, The EC50 of human ErbB4-Fc is 0.19-0.43 ug/ml.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag)(10108-H05H) at 2 ug/mL (100 uL/well) can bind SARS-CoV-2 (2019-nCoV) Spike RBD-His (F342L) Recombinant Protein (40592-V08H6), the EC50 of SARS-CoV-2 (2019-nCoV) Spike RBD-His (F342L) Recombinant Protein (40592-V08H6) is 20-50 ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag)(10108-H05H) at 2 ug/mL (100 uL/well) can bind SARS-CoV-2 (2019-nCoV) Spike RBD(G476S)-His Recombiant Protein (40592-V08H8), the EC50 of SARS-CoV-2 (2019-nCoV) Spike RBD(G476S)-His Recombiant Protein (40592-V08H8) is 25-60 ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized Human ACE2 (mFc tag) at 2 ug/mL (100 uL/well) can bind SARS-CoV-2 (2019-nCoV) Spike RBD(F490L)-His, the EC50 of SARS-CoV-2 (2019-nCoV) Spike RBD(F490L)-His is 30-150ng/mL.)
Application Data (Immobilized ACE2 Protein, Human, Recombinant (mFc Tag) at 2 ug/mL (100 uL/well) can bind SARS-CoV-2 (2019-nCoV) Spike RBD(E484K)-His, the EC50 of SARS-CoV-2 (2019-nCoV) Spike RBD(E484K)-His is 7-42ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized ACE2 Protein, Human, Recombinant (mFc Tag)(10108-H05H) at 2ug/mL (100uL/well) can bind SARS-CoV Spike S1+S2 ECD-His Recombinant Protein (S577A, Isolate Tor2)(40634-V08B),the EC50 of SARS-CoV Spike S1+S2 ECD-His Recombinant Protein (S577A, Isolate Tor2)(40634-V08B) is 300-900ng/mL.)
Application Data (Measured by its ability to inhibit Topflash reporter activity in HEK293T human embryonic kidney cells. The ED50 for this effect is typically 0.4-2 ug/mL in the presence of 300 ng/mL of Recombinant Mouse Wnt-3a.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized mouse F7-his at 10 ug/ml (100 ul/well) can bind biotinylated mouse F3-his. The EC50 of biotinylated mouse F3-his is 0.1-0.3 ug/ml.)
Application Data (Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.1-0.6 ng/mL.)
SDS-PAGE (Measured by its ability to inhibit the mouse CSF-induced proliferation of M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is typically 30-140ng/mL in the presence of 15 ng/mL mouse M-CSF.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized recombinant Mouse/Human BDNF Protein (Native) at 10 ug/ml (100 ul/well) can bind biotinylated mouse TrkB-His with a linear range of 10-80 ng/ml.)
Application Data (Measured by its ability to inhibit FGF acidic (aFGF / FGF1) dependent proliferation of Balb/c3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.1-0.6 ug/mL.)
Proteins are large molecules made up of long chains of amino acids.
They will typically fold into a very particular 3-dimensional shape/conformation, that is sometimes referred to as their “native” form, which allows them to work properly in the body. For the purposes of product categorization, AAA Biotech will typically refer to proteins purified from their original animal host as being “native” proteins (this is to signify their difference compared to their “recombinant” or “synthetic” protein counterparts).
If a protein successfully folds into the correct shape, it is will typically display high fidelity characteristics to its original protein in its original animal host, and be classified as an active protein, as it will be able to function “normally” in most enzymatic or binding capacities. If it loses this shape, due to factors such as heat or strong chemicals (such as detergents), it becomes inactive and is no longer able to perform its basic functions. All of the proteins in this category are made under strict quality control, and they are active, pure, low in contaminants, and stable.
Most are stored as freeze-dried powders and come without extra tags, so they’re very close to the actual natural/native form.
Key Applications of Active Proteins
1. Scientific Research
Aid in the study of how proteins function in the body
Aid in understanding various disease processes
2. Drug Development
Powerful tools to investigate how potential drugs interact with specific proteins
Ideal for identifying drug targets
3. Cell Culture
Are routinely utilized to support cell growth and function (e.g., using exogenous growth factors)
Can be used to promote cellular development into specific types (differentiation)
4. Diagnostics
Regularly utilized in tests to detect diseases or infections (e.g., COVID-19, cancer)
Note: All products are strictly for research-use only (RUO).
5. Therapeutics
Some active proteins are used directly as treatments (e.g., insulin, enzymes)
Note: All products are strictly for research-use only (RUO).
6. Vaccine Development
Used to create or test vaccines by mimicking parts of viruses or bacteria
7. Biochemical Assays
They can facilitate the characterization of enzyme activity, binding strength, or protein interactions in lab tests
Why Buy Active Proteins from AAA Biotech?
High biological activity – Verified to perform as expected or indicated on datasheet
Strict quality control – We are confident in our active proteins’ reliability and consistency
High purity & low endotoxin – Ideal for applications involving sensitive or precious samples/components
Freeze-dried for stability – Long shelf life and straightforward storage
Mostly tag-free – Closer to natural/native protein form
FAQ
1. What are active proteins used for in research?
Active proteins are used primarily in the study of how proteins function, in characterizing/discovering drug interactions, supporting cell growth, running biochemical assays, and in development of diagnostics or therapeutics.
2. How are AAA Biotech's active proteins validated?
AAA Biotech’s active proteins are validated through strict quality control and functional assays to ensure they are properly folded and active. “Active”, though, can be an ambiguous term, so if a specific “activity” or “binding” capability of a protein is of crucial interest to you, please inquire with us prior to purchase, and we will provide further details on how the “Active” modifier was determined to be applicable.
3. Are these proteins tested for biological activity?
Yes, all active proteins from AAA Biotech are tested to confirm they have the expected biological activity before being offered for use. Though, said “biological activity” can be either “enzymatic”, “binding”, or both.
Submit a Question
Please complete the form below and a representative will contact you as soon as possible.
Request more Information
Please complete the form below and a representative will contact you as soon as possible.
Request a Manual
Please complete the form below and a representative will contact you as soon as possible.
Request a Quote
Please complete the form below and a representative will contact you as soon as possible.