AAA Biotech provides a variety of high-quality recombinant and natural/native proteins that are proven to work in a wide range of experiments. Explore our products to find the active protein that best fits your needs or experimental model.
Application Data (Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is typically 2-10 ng/ml.)
Application Data (Measured in a cell proliferation assay using BaF3 mouse pro-B cells transfected with mouse Flt-3. The ED50 for this effect is typically 7-30 ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized mouse CD320-His at 10 ug/ml (100 ul/well) can bind biotinylated mouse TCN2-His, The EC50 of biotinylated mouse TCN2-His is 0.10-0.24 ug/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized mouse EPHB3-His at 10 ug/mL (100 uL/well) can bind mouse EFNB3-Fc, the EC50 of mouse EFNB3-Fc is 1-50 ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized mouse EFNB2-His at 10 ug/mL (100 uL/well) can bind mouse EPHB2-Fc. The EC50 of mouse EPHB2-Fc is 13.2-30.8 ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized mouse CD47-His at 10ug/mL (100uL/well) can bind mouse SIRPA-Fc, the EC50 of mouse SIRPA-Fc is 0.07-0.3 ug/mL.)
Application Data (Measured by its ability to induce alkaline phosphatase production by ATDC5 mouse chondrogenic cells. The ED50 for this effect is typically 0.5-3 ug/mL.)
Application Data (Measured in a serum-free cell proliferation assay using MCF-7 human breast cancer cells. The ED50 for this effect is typically 0.1-1 ug/mL.)
Application Data (Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED50 for this effect is typically 1-5 ng/ml.)
Application Data (Immobilized Cynomolgus SLC39A6/LIV-1 His at 2 ug/mL (100 uL/well) can bind Rabbit Anti-SLC39A6/LIV-1 Antibody, the EC50 of Rabbit Anti-SLC39A6/LIV-1 Antibody is 15-100 ng/mL.)
Bioactivity (Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag) at 2 ug/mL (100 uL/well) can bind SARS-CoV-2 (2019-nCoV) Spike RBD-AVI & His Recombinant Protein, Biotinylated, the EC50 of SARS-CoV-2 (2019-nCoV) Spike RBD-AVI & His Recombinant Protein, Biotinylated is 40-130 ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized Human CD200R His at 2 ug/ml (100 ul/well) can bind Human CD200 hFc, the EC50 of Human CD200 hFc is 1.0-7.0 ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human CD58-His at 10 ug/mL (100 uL/well) can bind human CD2-Fc, the EC50 of human CD2-Fc is 100-350 ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human VLDLR-His at 10ug/mL (100uL/well) can bind biotinylated human LRPAP1-His, the EC50 of biotinylated human LRPAP1-His is 0.05-0.2 ug/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human LRPAP1 at 0.5 ug/ml can bind human VLDLR with a range of 3.2-400 ng/ml.)
Application Data (Loaded Anti-CD97 Antibody on AMC Biosensor, can bind CD97 Protein, Human, Recombinant (His Tag) with an affinity constant of 0.2 nM as determined by Octet RED System.)
Application Data (Using the Octet RED System, the affinity constant (Kd) of CD97 Protein, Human, Recombinant (His Tag) bound to Anti-CD97 Antibody was 0.1 nM.)
Application Data (Measured by its binding ability in a functional ELISA. 1. Immobilized Rhesus ErbB3 at 2 ug/mL (100 ul/well) can bind human NRG1 (isoform Beta1), The EC50 of human NRG1 (isoform Beta1) is 0.58 ug/mL. 2. Immobilized human ErbB3 at 2 ug/mL (100 ul/well) can bind human NRG1 (isoform Beta1), The EC50 of human NRG1 (isoform Beta1) is 0.43 ug/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human CEACAM6-his at 10 ug/mL (100 ul/well) can bind biotinylated human CEACAM8-his, The EC50 of biotinylated human CEACAM8-his is 0.17 ug/mL.)
Application Data (Measured in a cell proliferation assay using M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is typically 1.6-6.3 ng/mL.)
Application Data (Measured in antiviral assays using WISH cells infected with vesicular stomatitis virus. The ED50 for this effect is typically 4-16 ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human Leptin at 1.25 ug/ml (100 ul/well) can bind human Leptin receptor with a linear range of 0.032-4 ug/ml.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized TGFBR2h (1-166Q) at 10 ug/mL (100 uL/well) can bind TGFB1-His/Biotin, the EC50 of human TGFB1-His/Biotin is 0.50-1.20 ug/mL.)
Application Data (Immobilized Cynomolgus mFc-TNFSF12 at 10 ug/ml (100 ul/well) can bind human Fc-TNFRSF12A, The ED50 of human Fc-TNFRSF12A is 0.07-0.15 ug/ml.)
Application Data (Measured by its ability to inhibit rhBMP4-induced alkaline phosphatase production by MC3T3-E1 mouse osteoblastic cells. The ED50 for this effect is typically 2-8 ug/mL in the presence of 50 ng/mL of recombinant human BMP4.)
Application Data (Measured in a cell proliferation assay using Balb/3T3 mouse embryonic fibroblast cells. The ED50 for this effect is typically 2-8 ug/mL.)
Application Data (Immobilized human ulBP1-His at 10 ug/ml (100 ul/well) can bind human S4-Fc3L3-NKG2D, The EC50 of human S4-Fc3L3-NKG2D is 0.39-0.91 ug/ml.)
Application Data (Loaded Fc-DPPIV on ProA Biosensor, can bind MERS-CoV Spike/S1 Protein (S1 Subunit, aa 1-725, His Tag) with an affinity constant of 0.02 nM as determined by Octet RED System.)
Application Data (Loaded Fc-DPPIV on ProA Biosensor, can bind MERS-CoV Spike/RBD Protein fragment (RBD, aa 367-606, His Tag) with an affinity constant of 1.04 nM as determined by Octet RED System.)
Application Data (Measured by its ability to inhibit IL-2 secretion in human T lymphocytes induced by anti-CD3 antibody and anti-CD28 antibody. The ED50 for this effect is 0.7-3.5 ug/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human 2B4-His, at 10 ug/mL (100 uL/well) can bind human CD48-Fc. The EC50 of human CD48-Fc is 0.03-0.06 ug/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human CEACAM6-his at 10 ug/mL (100 ul/well) can bind biotinylated human CEACAM8-his, The EC50 of biotinylated human CEACAM8-his is 0.17 ug/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human TNFRSF9 at 10 ug/mL (100 uL/well) can bind human TNFSF9, the EC50 of human TNFSF9 is 7-40ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (His tag) at 2ug/mL (100uL/well) can bind SARS-CoV-2 (2019-nCoV) Spike Protein (S1 Subunit, Fc Tag)(40591-V02H), the EC50 of SARS-CoV-2 (2019-nCoV) Spike Protein (S1 Subunit, Fc Tag) is 10-50 ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (His tag)(10108-H08H) at 2 ug/mL (100 uL/well) can bind SARS-CoV-2 (2019-nCoV) Spike S1(D614G), ECD-Fc(40591-V02H3), the EC50 of SARS-CoV-2 (2019-nCoV) Spike S1(D614G),ECD-Fc(40591-V02H3) is 40-230 ng/mL.)
Application Data (Loaded ACE2 Protein, Human, Recombinant (mFc Tag) on AMC Biosensor, can bind SARS-CoV-2 (2019-nCoV) Spike S1(D614G)-His Recombinant Protein with an affinity constant of 57.7 nM as determined by Octet RED System.)
Application Data
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag)(10108-H05H) at 2 ug/mL (100 uL/well) can bind SARS-CoV-2 (2019-nCoV) Spike S1(D614G)-His Recombinant Protein (40591-V08H3), the EC50 of SARS-CoV-2 (2019-nCoV) Spike S1(D614G)-His Recombinant Protein (40591-V08H3) is 250-650 ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (His tag) at 2ug/mL (100uL/well) can bind S3-2019-nCov-2-RBD(319R-541F,N501Y)-Fc, the EC50 of S3-2019-nCov-2-RBD(319R-541F,N501Y)-Fc is 1.5-8.0 ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag)(10108-H05H) at 2 ug/mL (100 uL/well) can bind SARS-CoV-2 (2019-nCoV) Spike RBD-His (N354D) Recombinant Protein (40592-V08H2), the EC50 of SARS-CoV-2 (2019-nCoV) Spike RBD-His (N354D) Recombinant Protein (40592-V08H2) is 20-50 ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag)(10108-H05H) at 2 ug/mL (100 uL/well) can bind SARS-CoV-2 (2019-nCoV) Spike RBD-His (V483A) Recombinant Protein (40592-V08H5), the EC50 of SARS-CoV-2 (2019-nCoV) Spike RBD-His (V483A) Recombinant Protein (40592-V08H5) is 20-50 ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized Human ACE2 (mFc tag) at 2 ug/mL (100 uL/well) can bind SARS-CoV-2 (2019-nCoV) Spike RBD(E484Q)-His, the EC50 of SARS-CoV-2 (2019-nCoV) Spike RBD(E484Q)-His is 5.0-25.0 ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag) at 2 ug/mL (100 uL/well) can bind SARS-CoV-2 (2019-nCoV) Spike RBD(K417N, E484K, N501Y)-His (40592-V08H85), the EC50 of SARS-CoV-2 (2019-nCoV) Spike RBD(K417N, E484K, N501Y)-His (40592-V08H85) is 5-40 ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized Human ACE2 (mFc tag) at 2 ug/mL (100 uL/well) can bind SARS-CoV-2 (2019-nCoV) Spike RBD (K417T, E484K, N501Y) Protein (His Tag), the EC50 of SARS-CoV-2 (2019-nCoV) Spike RBD (K417T, E484K, N501Y) Protein (His Tag) is 3.0-15.0 ng/mL.)
Application Data (Measured by its ability to inhibit BMP4-induced alkaline phosphatase production by MC3T3E1 mouse preosteoblast cells.The ED50 for this effect is 1.5-7.5 ug/mL.)
Application Data (Measured by its binding ability in a functional ELISA. 1. Immobilized human BID at 10 ug/mL (100 ul/well) can bind biotinylated mouse BCL2L1, The EC50 of biotinylated mouse BCL2L1 is 5.6 ng/mL. 2. Immobilized mouse BID at 10 ug/mL (100 ul/well) can bind biotinylated mouse BCL2L1, The EC50 of biotinylated mouse BCL2L1 is 7.1 ng/mL.)
Proteins are large molecules made up of long chains of amino acids.
They will typically fold into a very particular 3-dimensional shape/conformation, that is sometimes referred to as their “native” form, which allows them to work properly in the body. For the purposes of product categorization, AAA Biotech will typically refer to proteins purified from their original animal host as being “native” proteins (this is to signify their difference compared to their “recombinant” or “synthetic” protein counterparts).
If a protein successfully folds into the correct shape, it is will typically display high fidelity characteristics to its original protein in its original animal host, and be classified as an active protein, as it will be able to function “normally” in most enzymatic or binding capacities. If it loses this shape, due to factors such as heat or strong chemicals (such as detergents), it becomes inactive and is no longer able to perform its basic functions. All of the proteins in this category are made under strict quality control, and they are active, pure, low in contaminants, and stable.
Most are stored as freeze-dried powders and come without extra tags, so they’re very close to the actual natural/native form.
Key Applications of Active Proteins
1. Scientific Research
Aid in the study of how proteins function in the body
Aid in understanding various disease processes
2. Drug Development
Powerful tools to investigate how potential drugs interact with specific proteins
Ideal for identifying drug targets
3. Cell Culture
Are routinely utilized to support cell growth and function (e.g., using exogenous growth factors)
Can be used to promote cellular development into specific types (differentiation)
4. Diagnostics
Regularly utilized in tests to detect diseases or infections (e.g., COVID-19, cancer)
Note: All products are strictly for research-use only (RUO).
5. Therapeutics
Some active proteins are used directly as treatments (e.g., insulin, enzymes)
Note: All products are strictly for research-use only (RUO).
6. Vaccine Development
Used to create or test vaccines by mimicking parts of viruses or bacteria
7. Biochemical Assays
They can facilitate the characterization of enzyme activity, binding strength, or protein interactions in lab tests
Why Buy Active Proteins from AAA Biotech?
High biological activity – Verified to perform as expected or indicated on datasheet
Strict quality control – We are confident in our active proteins’ reliability and consistency
High purity & low endotoxin – Ideal for applications involving sensitive or precious samples/components
Freeze-dried for stability – Long shelf life and straightforward storage
Mostly tag-free – Closer to natural/native protein form
FAQ
1. What are active proteins used for in research?
Active proteins are used primarily in the study of how proteins function, in characterizing/discovering drug interactions, supporting cell growth, running biochemical assays, and in development of diagnostics or therapeutics.
2. How are AAA Biotech's active proteins validated?
AAA Biotech’s active proteins are validated through strict quality control and functional assays to ensure they are properly folded and active. “Active”, though, can be an ambiguous term, so if a specific “activity” or “binding” capability of a protein is of crucial interest to you, please inquire with us prior to purchase, and we will provide further details on how the “Active” modifier was determined to be applicable.
3. Are these proteins tested for biological activity?
Yes, all active proteins from AAA Biotech are tested to confirm they have the expected biological activity before being offered for use. Though, said “biological activity” can be either “enzymatic”, “binding”, or both.
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