AAA Biotech provides a variety of high-quality recombinant and natural/native proteins that are proven to work in a wide range of experiments. Explore our products to find the active protein that best fits your needs or experimental model.
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (His tag) at 2ug/mL (100uL/well) can bind SARS-CoV Spike/RBD Protein (RBD, mFc Tag) (40150-V05H), the EC50 of SARS-CoV Spike/RBD Protein (RBD, mFc Tag) is 9-40 ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (His tag) at 2 ug/mL (100 uL/well) can bind SARS-CoV Spike/S1 Protein (S1 Subunit, mFc Tag) (40150-V05H1), the EC50 of SARS-CoV Spike/S1 Protein (S1 Subunit, mFc Tag)is 50-120 ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized ACE2 Protein, Human, Recombinant (mFc Tag)(10108-H05H) at 2 ug/mL (100 uL/well) can bind SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD-AVI & His Recombiant Protein, Biotinylated (40589-V27B-B), the EC50 of SARS-CoV-2 (2019-nCoV) Spike S1+S2 ECD-AVI & His Recombiant Protein, Biotinylated (40589-V27B-B) is 600-900 ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human ACE2 protein (Fc tag)(10108-H05H) at 2 ug/mL (100 uL/well) can bind SARS-CoV-2 (2019-nCoV) Spike RBD-His (A435S) Recombinant Protein (40592-V08H4),the EC50 of SARS-CoV-2 (2019-nCoV) Spike RBD-His (A435S) Recombinant Protein (40592-V08H4) is 20-50 ng/mL.)
SDS-PAGE (Measured by its binding ability in a functional ELISA. Immobilized EFNA1-Fc at 10 ug/mL (100 uL/well) can bind mEPHA2-His/Biotin, the EC50 of mEPHA2-His/Biotin is 60-140 ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized recombinant human EphrinB1 at 10 ug/ml (100 ul/well) can bind human EphB6 with a linear range of 0.16-4 ug/ml.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized ALK4-His at 10 ug/mL (100 uL/well) can bind TDGF1-Fc, the EC50 of human TDGF1-Fc is 6.0-14 ng/mL.)
Application Data (Measured by its ability to induce activation of beta-catenin response in a Topflash Luciferase assay using HEK293T human embryonic kidney cells. The ED50 for this effect is typically 0.1-0.5 ug/mL in the presence of 5 ng/mL recombinant mouse Wnt3a.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized Human RSPO3 His at 2 ug/ml (100 ul/well) can bind Human RNF43 hFc, the EC50 of Human RNF43 hFc is 3.0-12.0 ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized Human TGF-alpha at 2 ug/ml (100 ul/well) can bind Human EGFR hFc, the EC50 of Human EGFR hFc is 80-500 ng/mL.)
Application Data (Measured in a cell proliferation assay using Balb/c 3T3 mouse embryonic fibroblast cells. The ED50 for this effect is 0.5-2.5 ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human CD55 at 2 ug/ml (100 ul/well) can bind human CD97 with a linear ranger of 1.28-32 ng/ml.)
Application Data (Immobilized NECTIN4/Nectin 4 Protein, Human, Recombinant (ECD, His Tag) at 2 ug/ml (100 ul/well) can bind PVRL1/NECTIN1 Protein, Human, Recombinant (hFc Tag), the EC50 of PVRL1/NECTIN1 Protein, Human, Recombinant (hFc Tag)is 20-120 ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized rh PVRL3 at 1 ug/ml (100 ul/well) can bind recombinant human Nectin-1 with a linear range of 0.156-5 ng/ml.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human CD47-His at 2 ug/ml (100 ul/well) can bind human SIRPA-Fc, the EC50 of human SIRPA-Fc is 3-15 ng/mL.)
Application Data (Measured by the ability of the immobilized protein to enhance BMP2-induced alkaline phosphatase activity in C3H10T1/2 mouse embryonic fibroblast cells. The ED50 for this effect is typically 2-20 ug/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human BTLA-Fc at 10ug/mL (100uL/well) can bind biotinylated mouse HVEM-Fch, the EC50 of biotinylated mouse HVEM-Fch is 6-70 ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human ErbB2 at 2.5 ug/ml can bind Herceptin with a linear range of 3.2-80 ng/ml.)
Application Data (Measured by its ability to block anti-ErbB2 mediated inhibition of BT474 human breast ductal carcinoma cell proliferation. The ED50 for this effect is 0.5-3 ug/mL in the presence of 0.6 ug/mL Anti-ErbB2/Her2 Monoclonal Antibody.)
Application Data (Using the Octet RED System, the affinity constant (Kd) of Her2 / ERBB2 Protein, Human, Recombinant, Biotinylated bound to Pertuzumab was 0.1 nM.)
Application Data (Immobilized PVRIG Protein, Cynomolgus, Rhesus, Recombinant (His Tag) at 2 ug/ml (100 ul/well) can bind Nectin-2 Protein, Human, Recombinant (hFc Tag), the EC50 of Nectin-2 Protein, Human, Recombinant (hFc Tag)is 0.2-1.2 ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human CD226 at 2 ug/ml (100 ul/well) can bind human CD112 with a linear range of 6.4-800 ng/ml.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human VEGF121 at 10 ug/mL (100 uL/well) can bind human VEGFR2-Fc, the EC50 of human VEGFR2-Fc is 5-50 ng/mL.)
Application Data (Measured by its ability to inhibit VEGF-dependent proliferation of human umbilical vein endothelial cells (HUVEC) in the presence of 10 ng/mL rhVEGF165. The ED50 for this effect is 40-120ng/mL.)
Application Data (Immobilized TIGIT Protein, Human, Recombinant (ECD, His Tag) at 2 ug/ml (100 ul/well) can bind CD155/PVR Protein, Human, Recombinant (ECD, hFc Tag), the EC50 of CD155/PVR Protein, Human, Recombinant (ECD, hFc Tag) is 25-140 ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human CD226 His at 2 ug/mL (100 ul/well) can bind human CD155 hFc, The EC50 of bind human CD155 hFc is 100-800 ng/mL.)
Application Data (Measured by its ability to inhibit VEGF-dependent proliferation of human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 20-100 ng/mL in the presence of 10 ng/mL rhVEGF165.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human VEGFR1-Fc at 10 ug/mL (100 uL/well) can bind biotinylated human VEGF165, the EC50 of biotinylated human VEGF165 is 10-40 ng/mL.)
SDS-PAGE (Measured by its ability to inhibit the human CSF-induced proliferation of M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is typically 0.05-0.3ug/mL in the presence of 3 ng/ml Recombinant Human M-CSF.)
Application Data (Measured by its ability to inhibit the human CSF-induced proliferation of M-NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is typically 0.4-1.6ug/mL in the presence of 3 ng/ml Recombinant Human M-CSF.)
Application Data (Measured by its ability to inhibit BMP4-induced activity in MC3T3-E1 Mouse osteoblastic cells. The ED50 for this effect is typically 0.2-1.2 ug/ml in the presence of 50 ng/mL of recombinant human BMP4.)
Application Data (Measured by its ability to inhibit BMP4-induced activity in MC3T3-E1 Mouse osteoblastic cells. The ED50 for this effect is typically 5-20 ug/ml in the presence of 50 ng/mL of recombinant human BMP4.)
Application Data (Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T1/2 cells. The ED50 for this effect is approximately 0.01-0.07ug/mL in the presence of 10 ng/mL of mouse Wnt3a.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized recombinant human DR6-his at 10 ug/mL (100 ul/well) can bind biotinylated human APP-Fc with a linear range of 0.0125-0.4 ug/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized recombinant human EphB6 at 10 ug/ml (100 ul/well) can bind human EphrinB2 with a linear range of 1.28-32 ng/ml.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized recombinant human EphB6 at 10 ug/ml (100 ul/well) can bind human EphrinB1 with a linear range of 32-800 ng/ml.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human PGF-mFc at 10 ug/mL (100 uL/well) can bind human VEGFR1-His. The EC50 of human VEGFR1-His is 0.19-0.43 ug/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human NT3 at 2 ug/ml (100 ul/well) can bind human TrkB-Fch. The EC50 of human TrkB-Fch is 120-350 ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human MDK at 10 ug/ml (100 ul/well) can bind mouse SDC4-Fc with a linear range of 0.16-1.25 ug/ml.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized mouse His-TNFSF9 at 10 ug/ml (100 ul/well) can bind mouse TNFRSF9-Fc, The EC50 of mouse TNFRSF9-Fc is 12.0-29.0 ng/ml.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized mouse EPHA1-His at 10 ug/ml (100 ul/well) can bind mouse EFNA1-Fc, The EC50 of mouse EFNA1-Fc is 21.3-49.8 ng/ml.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized rat CD47-His at 10 ug/ml (100 ul/well) can bind mouse SIRPA-Fc, The EC50 of mouse SIRPA-Fc is 0.6-1.4 ug/ml.)
Application Data (Measured by its ability to inhibit BMP4-induced alkaline phosphatase production by MC3T3E1 mouse preosteoblast cells.The ED50 for this effect is 3-9 ug/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized mouse ANGPT2 at 10 ug/mL (100 uL/well) can bind mouse TEK-Fc. The EC50 of mouse TEK-Fc is 0.58-1.34 ug/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized mouse EFNA5-His at 10 ug/ml (100 ul/well) can bind mouse EPHA3-Fc, The EC50 of rat mouse EPHA3-Fc is 12.3-28.9 ng/ml.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized mouse EPHA3-His at 10 ug/mL (100 uL/well) can bind mouse EFNA5-Fc. The EC50 of mouse EFNA5-Fc is 4.9-11.4 ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized mouse EPHB2-His at 10 ug/mL (100 uL/well) can bind mouse EFNB2-Fc. The EC50 of mouse EFNB2-Fc is 0.01-0.03 ug/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human EphB4-His at 10 ug/ml (100 ul/well) can bind zebrafish EFNB2A-Fc, The EC50 of zebrafish EFNB2A-Fc is 12.8-30.1 ng/ml.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human EphB4-His at 10 ug/mL (100 uL/well) can bind canine EFNB2-Fc3. The EC50 of canine EFNB2-Fc3 is 25.8-60.2 ng/mL.)
Proteins are large molecules made up of long chains of amino acids.
They will typically fold into a very particular 3-dimensional shape/conformation, that is sometimes referred to as their “native” form, which allows them to work properly in the body. For the purposes of product categorization, AAA Biotech will typically refer to proteins purified from their original animal host as being “native” proteins (this is to signify their difference compared to their “recombinant” or “synthetic” protein counterparts).
If a protein successfully folds into the correct shape, it is will typically display high fidelity characteristics to its original protein in its original animal host, and be classified as an active protein, as it will be able to function “normally” in most enzymatic or binding capacities. If it loses this shape, due to factors such as heat or strong chemicals (such as detergents), it becomes inactive and is no longer able to perform its basic functions. All of the proteins in this category are made under strict quality control, and they are active, pure, low in contaminants, and stable.
Most are stored as freeze-dried powders and come without extra tags, so they’re very close to the actual natural/native form.
Key Applications of Active Proteins
1. Scientific Research
Aid in the study of how proteins function in the body
Aid in understanding various disease processes
2. Drug Development
Powerful tools to investigate how potential drugs interact with specific proteins
Ideal for identifying drug targets
3. Cell Culture
Are routinely utilized to support cell growth and function (e.g., using exogenous growth factors)
Can be used to promote cellular development into specific types (differentiation)
4. Diagnostics
Regularly utilized in tests to detect diseases or infections (e.g., COVID-19, cancer)
Note: All products are strictly for research-use only (RUO).
5. Therapeutics
Some active proteins are used directly as treatments (e.g., insulin, enzymes)
Note: All products are strictly for research-use only (RUO).
6. Vaccine Development
Used to create or test vaccines by mimicking parts of viruses or bacteria
7. Biochemical Assays
They can facilitate the characterization of enzyme activity, binding strength, or protein interactions in lab tests
Why Buy Active Proteins from AAA Biotech?
High biological activity – Verified to perform as expected or indicated on datasheet
Strict quality control – We are confident in our active proteins’ reliability and consistency
High purity & low endotoxin – Ideal for applications involving sensitive or precious samples/components
Freeze-dried for stability – Long shelf life and straightforward storage
Mostly tag-free – Closer to natural/native protein form
FAQ
1. What are active proteins used for in research?
Active proteins are used primarily in the study of how proteins function, in characterizing/discovering drug interactions, supporting cell growth, running biochemical assays, and in development of diagnostics or therapeutics.
2. How are AAA Biotech's active proteins validated?
AAA Biotech’s active proteins are validated through strict quality control and functional assays to ensure they are properly folded and active. “Active”, though, can be an ambiguous term, so if a specific “activity” or “binding” capability of a protein is of crucial interest to you, please inquire with us prior to purchase, and we will provide further details on how the “Active” modifier was determined to be applicable.
3. Are these proteins tested for biological activity?
Yes, all active proteins from AAA Biotech are tested to confirm they have the expected biological activity before being offered for use. Though, said “biological activity” can be either “enzymatic”, “binding”, or both.
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