AAA Biotech provides a variety of high-quality recombinant and natural/native proteins that are proven to work in a wide range of experiments. Explore our products to find the active protein that best fits your needs or experimental model.
ELISA (Figure 2. PlGF Sandwich-ELISA using recombinant human PlGF-1 as standard. Mouse anti-human PlGF #178/G10 was used as capture antibody, Biotinylated rabbit anti-human PlGF was used for detection.)
SDS-PAGE (Fig. 1: SDS-PAGE analysis of recombinant human PlGF-1. Samples were loaded in 15% SDS-polyacrylamide gel under non-reducing conditions and stained with Coomassie blue.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human PD-L2 at 2 ug/ml (100 ul/well) can bind human PD-1, the EC50 of human PD-1 is 150-800 ng/mL.)
Application Data (Measured in a cell proliferation assay using Balb/C 3T3 mouse embryonic fibroblasts.The ED50 for this effect is typically 0.4-2ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized recombinant Human HVEM-His at 10 ug/ml (100 ul/well) can bind human BTLA-Fc with a linear range of 1.28-20 ug/ml.)
Application Data (Measured in a cell proliferation assay using human umbilical vein endothelial cells (HUVEC). The ED50 for this effect is typically 5-25 ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human BID at 10 ug/mL (100 ul/well) can bind biotinylated mouse BCL2L1, The EC50 of biotinylated mouse BCL2L1 is 5.6 ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human BID at 10 ug/mL (100 ul/well) can bind biotinylated human BCL2L1, The EC50 of biotinylated human BCL2L1 is 7.1 ng/mL.)
Application Data (Measured by its ability to inhibit FGF acidic (aFGF / FGF1) dependent proliferation of Balb/c3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 5-25ng/mL.)
Application Data (Measured by its ability to inhibit FGF acidic (aFGF / FGF1) dependent proliferation of Balb/c3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 0.2-1ug/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized VEGF C-his at 2 ug/mL (100 uL/well) can bind VEGFR3-His, Biotinylated, the EC50 of VEGFR3-His, Biotinylated is 1-10 ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized CD155a-His at 10 ug/mL (100 uL/well) can bind CD226-Fc-10565-H02H, the EC50 of human CD226-Fc is 15-40 ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human CD226 at 2 ug/ml (100 ul/well) can bind human CD112 with a linear ranger of 6.4-800 ng/ml.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized Human PDGF-B His at 2 ug/ml (100 ul/well) can bind Human PDGFRBHis & hFc, the EC50 of Human PDGFRBHis & hFc is 9.0-40.0 ng/mL.)
Application Data (Measured by its ability to inhibit FGF acidic dependent proliferation of Balb/C 3T3 mouse fibroblasts. The ED50 for this effect is typically 0.1-0.6 ug/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized Mouse TNFSF13 at 2 ug/mL (100 ul/well) can bind Human BCMA, The EC50 of Human BCMA is 0.6-2.1 ng/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized Human BAFF at 2 ug/mL (100 ul/well) can bind Human BCMA, The EC50 of Human BCMA is 30-80 ng/mL.)
Application Data (Immobilized human GST-SerpinB2 at 10 ug/ml (100 ul/well) can bind biotinylated human uPA-His, The EC50 of biotinylated human uPA-His is 10.24-23.88 ng/ml.)
SDS-PAGE (Measured by its ability to inhibit proliferation of HeLa human cervical epithelial carcinoma cells. The ED50 for this effect is typically 5-30 ug/ml.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human VEGFC-His at 10 ug/mL (100 uL/well) can bind human NRP2-Fc, the EC50 of human NRP2-Fc is 0.1-0.5 ug/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized B7-1 Protein, Human, Recombinant (His Tag) at 2 ug/ml (100 ul/well) can bind CTLA-4 Protein, Human, Recombinant (His & hFc Tag). The EC50 of of CTLA-4 (His & hFc Tag) is 35-80 ng/ml.)
Application Data (Measured by its ability to inhibit EPO-dependent proliferation of TF-1 human erythroleukemic cells. The ED50 for this effect is typically 15-60 ng/mL in the presence of 0.1 U/mL Recombinant Human EPO.)
Application Data (Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T 1/2 2A6 mouse embryonal fibroblast cells. The ED50 for this effect is typically 1-6 ug/mL in the presence of 20 ng/mL of Wnt3a.)
Application Data (Measured by its ability to inhibit Wnt3a-induced alkaline phosphatase production by C3H10T 1/2 2A6 mouse embryonal fibroblast cells. The ED50 for this effect is typically 2-20 ug/mL in the presence of 20 ng/mL of Wnt3a.)
Application Data (Immobilized human His-NKG2D (78-216) at 10 ug/ml (100 ul/well) can bind human MICB-Fch, The EC50 of human MICB-Fch is 15.9-37.1 ng/ml.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human CD48 at 10 ug/ml (100 ul/well) can bind recombinant human 2B4 / CD244 with a linear range of 0.004-0.4 ug/ml.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human VEGF-C at 10 ug/mL (100 ul/well) can bind human VEGFR3-his. The EC50 of human VEGFR3-his is 0.011 ug/mL.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized Human CD21 His at 2 ug/ml (100 ul/well) can bind Human Annexin VI/ANXA6 His, Biotinylated, the EC50 of Human Annexin VI/ANXA6 His, Biotinylated is 250-900 ng/mL.)
Application Data (Measured by its ability to inhibit FGF acidic dependent proliferation of Balb/c3T3 mouse embryonic fibroblasts. The ED50 for this effect is typically 200-400 ng/mL.)
Application Data (Measured by its ability to inhibit TNF-alpha mediated cytotoxicity in L-929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically2-10ng/mL in the presence of 0.25 ng/mL recombinant human TNF-alpha.)
Application Data (Measured by its ability to inhibit TNFalpha-mediated cytotoxicity in L929 mouse fibrosarcoma cells in the presence of metabolic inhibitor actinomycin D. The ED50 for this effect is typically 0.2-1ug/mL in the presence of 0.25 ng/mL recombinant human TNFalpha.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized human EFNB2 at 2 ug/ml (100 ul/well) can bind human EphB4 with a linear ranger of 1.56-12.5 ng/ml.)
Application Data (Measured by its binding ability in a functional ELISA. Immobilized Human EphA2 His at 2 ug/ml (100 ul/well) can bind Human Ephrin-A1 His & hFc, the EC50 of Human Ephrin-A1 His & hFc is 4.0-20.0 ng/mL.)
SDS-PAGE (Measured by its ability to inhibit FGF-acidic dependent proliferation of Balb/c 3T3 mouse fibroblasts. The ED50 for this effect is typically 0.5-4 ng/mL.)
SDS-PAGE (Measured by its ability to inhibit FGF-acidic dependent proliferation of Balb/c 3T3 mouse fibroblasts. The ED50 for this effect is typically 40-200 ng/mL.)
Influenza A H7N9 (A/Hangzhou/3/2013) Hemagglutinin/HA Protein (His Tag)
Reactivity
H7N9
Purity
>95% as determined by SDS-PAGE
Pricing
What Are Active Proteins?
Proteins are large molecules made up of long chains of amino acids.
They will typically fold into a very particular 3-dimensional shape/conformation, that is sometimes referred to as their “native” form, which allows them to work properly in the body. For the purposes of product categorization, AAA Biotech will typically refer to proteins purified from their original animal host as being “native” proteins (this is to signify their difference compared to their “recombinant” or “synthetic” protein counterparts).
If a protein successfully folds into the correct shape, it is will typically display high fidelity characteristics to its original protein in its original animal host, and be classified as an active protein, as it will be able to function “normally” in most enzymatic or binding capacities. If it loses this shape, due to factors such as heat or strong chemicals (such as detergents), it becomes inactive and is no longer able to perform its basic functions. All of the proteins in this category are made under strict quality control, and they are active, pure, low in contaminants, and stable.
Most are stored as freeze-dried powders and come without extra tags, so they’re very close to the actual natural/native form.
Key Applications of Active Proteins
1. Scientific Research
Aid in the study of how proteins function in the body
Aid in understanding various disease processes
2. Drug Development
Powerful tools to investigate how potential drugs interact with specific proteins
Ideal for identifying drug targets
3. Cell Culture
Are routinely utilized to support cell growth and function (e.g., using exogenous growth factors)
Can be used to promote cellular development into specific types (differentiation)
4. Diagnostics
Regularly utilized in tests to detect diseases or infections (e.g., COVID-19, cancer)
Note: All products are strictly for research-use only (RUO).
5. Therapeutics
Some active proteins are used directly as treatments (e.g., insulin, enzymes)
Note: All products are strictly for research-use only (RUO).
6. Vaccine Development
Used to create or test vaccines by mimicking parts of viruses or bacteria
7. Biochemical Assays
They can facilitate the characterization of enzyme activity, binding strength, or protein interactions in lab tests
Why Buy Active Proteins from AAA Biotech?
High biological activity – Verified to perform as expected or indicated on datasheet
Strict quality control – We are confident in our active proteins’ reliability and consistency
High purity & low endotoxin – Ideal for applications involving sensitive or precious samples/components
Freeze-dried for stability – Long shelf life and straightforward storage
Mostly tag-free – Closer to natural/native protein form
FAQ
1. What are active proteins used for in research?
Active proteins are used primarily in the study of how proteins function, in characterizing/discovering drug interactions, supporting cell growth, running biochemical assays, and in development of diagnostics or therapeutics.
2. How are AAA Biotech's active proteins validated?
AAA Biotech’s active proteins are validated through strict quality control and functional assays to ensure they are properly folded and active. “Active”, though, can be an ambiguous term, so if a specific “activity” or “binding” capability of a protein is of crucial interest to you, please inquire with us prior to purchase, and we will provide further details on how the “Active” modifier was determined to be applicable.
3. Are these proteins tested for biological activity?
Yes, all active proteins from AAA Biotech are tested to confirm they have the expected biological activity before being offered for use. Though, said “biological activity” can be either “enzymatic”, “binding”, or both.
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