At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.
Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.
IHC (Immunohiostchemistry) (AAA321912 at 1/100 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of EPHA3 expression in 293 cells., The lane on the left is treated with the antigen-specific peptide.)
IF (Immunofluorescence) (AAA321918 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
IF (Immunofluorescence) (AAA321918 staining K-562 cells by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) antibody, diluted at 1/600, was used as secondary antibody.)
WB (Western Blot) (Western blot analysis of hnRNP C1/C2 Antibody expression in 293 cells lysates.The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemistry) (AAA321918 at 1/100 staining human brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of CD69 expression in Jurkat cell lysate, The lane on the left is treated with the antigen-specific peptide.)
IF (Immunofluorescence) (AAA321931 staining HuvEc by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
WB (Western Blot) (Western blot analysis of CHOP expression in Jurkat cell lysate, The lane on the left is treated with the antigen-specific peptide.)
IF (Immunofluorescence) (AAA321936 staining Hela by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
IHC (Immunohistochemistry) (AAA321936 at 1/100 staining Human lung tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohiostchemistry) (AAA321403 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of extracts of mouse brain tissue, using Phospho-Tau (Ser202) Antibody.)
IHC (Immunohiostchemistry) (AAA321410 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of extracts of THP-1 cells, using Phospho-TRAF2 (Ser11) Antibody.)
WB (Western Blot) (Western blot analysis of extracts from HT29 cells plpC-transfected, using Phospho-IRF3(Ser396) Antibody.)
IHC (Immunohiostchemistry) (AAA321418 at 1/100 staining Mouse thymus tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
IF (Immunofluorescence) (AAA321418 staining HepG2 cells(30min of 4uM Forskolin treatment) by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab(AAA321418) and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary antibody.The nuclear counter stain is DAPI(blue).)
The Ab is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
IHC (Immunohiostchemistry) (AAA321424 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of extracts of Ramos Jurkat cells, using Phospho-PLCG2(Tyr1217) Antibody.)
IHC (Immunohistochemisry) (AAA321432 at 1/100 staining Human normal tissues adjacent tomammary cancer by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary antibody at 4°C overnight. An HRP conjugated anti-Rabbit antibody was used as the secondary antibody.)
WB (Western Blot) (Western blot analysis of Phospho-IKK alpha/ beta (Ser180/181) Antibody expression in TNF treated HepG2 cells lysates.Thelane on the right was treated with the antigen-specific peptide.)
IF (Immunofluorescence) (AAA321432 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
WB (Western Blot) (Western blot analysis of Phospho-EGFR (Thr678) expression in various lysates)
WB (Western Blot) (Western blot analysis of EGFR phosphorylation expression in EGF treated COS7 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
IF (Immunofluorescence) (AAA321447 staining SK-OV3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
IF (Immunofluorescence) (AAA321447 staining BT-20 cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondary antibody.)
WB (Western Blot) (Western blot analysis of Phospho-EGFR (Tyr1092) expression in various lysates)
WB (Western Blot) (Western blot analysis of EGFR phosphorylation expression in EGF treated HuvEc whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohiostchemistry) (AAA321450 at 1/200 staining human colon carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IF (Immunofluorescence) (AAA321450 staining SK-OV3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
WB (Western Blot) (Western blot analysis of Phospho-EGFR (Tyr869) expression in various lysates)
WB (Western Blot) (Western blot analysis of EGFR phosphorylation expression in EGF treated A431 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
IF (Immunofluorescence) (AAA321454 staining SK-OV3 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
WB (Western Blot) (Western blot analysis of Estrogen Receptor alpha phosphorylation expression in 293 whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohiostchemistry) (AAA321458 at 1/100 staining human Breast carcinoma tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IF (Immunofluorescence) (AAA321458 staining 293 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
WB (Western Blot) (Western blot analysis of p53 phosphorylation expression in LOVO whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohiostchemistry) (AAA321466 at 1/200 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IF (Immunofluorescence) (AAA321466 staining MDA-MB-435 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
WB (Western Blot) (Western blot analysis of p53 phosphorylation expression in ovary cancer whole cell lysates, The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohiostchemistry) (AAA321468 at 1/200 staining human kidney sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IF (Immunofluorescence) (AAA321468 staining MDA-MB-435 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
IHC (Immunohistochemisry) (AAA321796 at 1/100 staining human colon tissues sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at121 degree)
WB (Western Blot) (Western blot analysis of Rab5A expression in HeLa cell lysate, The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemistry) (AAA321796 at 1/100 staining Human prostate tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemisry) (AAA321819 at 1/100 staining human breast tissues sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at103)
IHC (Immunohiostchemistry) (AAA321819 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of AFP expression in hepatocyte lysates., The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemisry) (AAA321820 at 1/100 staining human breast tissues sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at104)
IHC (Immunohiostchemistry) (AAA321820 at 1/100 staining Mouse liver tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of Bag 1 expression in HeLa cell lysate, The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemisry) (AAA321825 at 1/100 staining human Lymph node tissues sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at)
WB (Western Blot) (Western blot analysis of Interleukin 8 expression in HeLa cells, The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemistry) (AAA321825 at 1/100 staining Human Melanoma tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of Integrin alphaV expression in HeLa cells, The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemisry) (AAA321830 at 1/100 staining human lung tissues sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at144 degree C.)
IHC (Immunohiostchemistry) (AAA321830 at 1/100 staining Human prostate tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot (WB) analysis of Integrin ?V expression in HeLa cells. The lane on the left was treated with the antigen-specific peptide.)
IHC (Immunohistochemisry) (AAA321834 at 1/100 staining Rat brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the primary Ab at 4°C overnight. An HRP conjugated anti-Rabbit Ab was used as the secondary Ab.)
IHC (Immunohiostchemistry) (Annexin A3 Antibody for IHC in human kidney tissue)
WB (Western Blot) (Western blot analysis of Annexin III Antibody expression in rat liver tissue lysates.The lane on the right is treated with the antigen-specific peptide.)
WB (Western Blot) (Western blot analysis of Smad 6 expression in mouse brain, The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemistry) (AAA321835 at 1/100 staining human lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IHC (Immunohistochemistry) (AAA321836 at 1/100 staining human Pancreas cancer tissues sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hou)
WB (Western Blot) (Western blot analysis of Lamin B expression in COLO cells, The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohiostchemistry) (AAA321836 at 1/100 staining Mouse brain tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IF (Immunofluorescence) (AAA321836 staining lovo cells by ICC/IF. Cells were fixed with PFA and permeabilized in 0.1% saponin prior to blocking in 10% serum for 45 minutes at 37 degree C. The primary antibody was diluted 1/400 and incubated with the sample for 1 hour at 37 degree C. A Alexa Fluor 594 conjugated goat polyclonal to rabbit IgG (H+L), diluted 1/600 was used as secondry antibody)
WB (Western Blot) (Western blot analysis of Apolipoprotein E expression in RAW264.7 cells, The lane on the left is treated with the antigen-specific peptide.)
IF (Immunofluorescence) (AAA321848 staining K562 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
IHC (Immunohistochemistry) (AAA321848 at 1/100 staining human brain tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of Integrin alpha3 (CD49c) expression in HepG2 cell, The lane on the left is treated with the antigen-specific peptide.)
WB (Western Blot) (Western blot analysis of extracts of various celllines, using Integrin alpha3 (CD49c) Antibody.)
IF (Immunofluorescence) (AAA321852 staining HepG2 by IF/ICC. The sample were fixed with PFA and permeabilized in 0.1% Triton X-100, then blocked in 10% serum for 45 minutes at 25 degree C. The primary antibody was diluted at 1/200 and incubated with the sample for 1 hour at 37 degree C. An Alexa Fluor 594 conjugated goat anti-rabbit IgG (H+L) Ab, diluted at 1/600, was used as the secondary antibody.)
WB (Western Blot) (Western blot analysis of Cathepsin B expression in COLO cells)
IHC (Immunohistochemistry) (AAA321858 at 1/100 staining human liver tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of ACE1 expression in mouse kidney, The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemistry) (AAA321865 at 1/100 staining human lung tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of caspase12 expression in HUVEC cells, The lane on the left is treated with the antigen-specific peptide.)
IHC (Immunohistochemistry) (AAA321867 at 1/100 staining human kidney tissue sections by IHC-P. The tissue was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The tissue was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of extracts of HeLa cells, using Phospho-CTDSPL2 (Ser104) Antibody.)
IHC (Immunohistochemistry) (AAA321317 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of extracts of MKN-45 cells treated with Forskolin, using Phospho-DARPP-32 (Ser97) Antibody.)
IHC (Immunohistochemistry) (AAA321320 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
IF (Immunofluorescence) (AAA321321 staining Heat-shock treated Hela cells by IF/ICC. The samples were fixed with PFA and permeabilized in 0.1% Triton X-100,then blocked in 10% serum for 45 minutes at 25°C. Samples were then incubated with primary Ab and mouse anti-beta tubulin Ab for 1 hour at 37°C. An AlexaFluor594 conjugated goat anti-rabbit IgG(H+L) Ab(Red) and an AlexaFluor488 conjugated goat anti-mouse IgG(H+L) Ab(Green) were used as the secondary Ab. The nuclear counter stain is DAPI (blue).)
WB (Western Blot) (Western blot analysis of extracts of T47-D cells treated with collagen, using Phospho-DDR1 (Tyr513) Antibody.)
IHC (Immunohistochemistry) (AAA321321 at 1/100 staining Human breast cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of extracts of serum-starved HeLa cells, using Phospho-eEF2k (Ser366) Antibody.)
IHC (Immunohistochemistry) (AAA321324 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of extracts of PC12 cells, using Phospho-eIF4G (Ser1147) Antibody.)
IHC (Immunohistochemistry) (AAA321325 at 1/100 staining Human thyroid cancer tissue by IHC-P. The sample was formaldehyde fixed and a heat mediated antigen retrieval step in citrate buffer was performed. The sample was then blocked and incubated with the antibody for 1.5 hours at 22 degree C. An HRP conjugated goat anti-rabbit antibody was used as the secondary.)
WB (Western Blot) (Western blot analysis of lysates from HeLa cells, using Tau (Phospho-Ser396) Antibody.)
ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using Tau (Phospho-Ser396) Antibody)
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
WB (Western Blot) (Western blot analysis of lysates from COS7 cells, treated with sorbitol 0.4M 24h, using NF-kappaB p65 Antibody. The lane on the right is blocked with the synthesized peptide.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using NF-kappaB p65 Antibody. The picture on the right is blocked with the synthesized peptide.)
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using NF-kappaB p65 Antibody. The picture on the right is blocked with the synthesized peptide.)
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu
Reactivity
Human, Mouse, Rat
Applications
Western Blot
Purity
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.
WB (Western Blot) (Western blot analysis of lysates from Jurkat cells, using EDG3 Antibody. The lane on the right is blocked with the synthesized peptide.)
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using EDG3 Antibody. The picture on the right is blocked with the synthesized peptide.)
WB (Western Blot) (Western blot analysis of lysates from K562 cells, using PE2R3 Antibody. The lane on the right is blocked with the synthesized peptide.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue, using PE2R3 Antibody. The picture on the right is blocked with the synthesized peptide.)
IF (Immunofluorescence) (Immunofluorescence analysis of COS7 cells, using PE2R3 Antibody. The picture on the right is blocked with the synthesized peptide.)
WB (Western Blot) (Western blot analysis of lysates from HT-29 cells, using GPR18 Antibody. The lane on the right is blocked with the synthesized peptide.)
IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using GPR18 Antibody. The picture on the right is blocked with the synthesized peptide.)
WB (Western Blot) (Western blot analysis of lysates from NIH/3T3 cells, using GPR157 Antibody. The lane on the right is blocked with the synthesized peptide.)
IF (Immunofluorescence) (Immunofluorescence analysis of HUVEC cells, using GPR157 Antibody. The picture on the right is blocked with the synthesized peptide.)
WB (Western Blot) (Western blot analysis of lysates from LOVO cells, using GPR20 Antibody. The lane on the right is blocked with the synthesized peptide.)
IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue, using GPR20 Antibody. The picture on the right is blocked with the synthesized peptide.)
IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells, using GPR20 Antibody. The picture on the right is blocked with the synthesized peptide.)
Immunofluorescence, Immunohistochemistry, Western Blot
Purity
Purified from rabbit antiserum by affinity-chromatography using immunogen.
Pricing
What are Polyclonal Antibodies?
Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).
Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.
Key Uses of Polyclonal Antibodies
Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.
Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.
ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.
Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.
Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.
Why Buy Polyclonal Antibodies from AAA Biotech?
1. Ideal for Various Applications
Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.
2. Rigorous Quality Control
All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.
3. Wide Assortment of Antibodies
Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.
4. Highly Purified
Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.
FAQ
1. How are polyclonal antibodies produced?
Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.
2. How do polyclonal antibodies differ from monoclonal antibodies?
Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.
3. How should I store polyclonal antibodies?
Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.
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