Polyclonal Antibodies

At AAA Biotech, we provide a broad range of purified polyclonal antibodies (pAbs) that are able to all be browsed online through our website. Due to their high specificity and strong binding affinity, these antibodies are ideal for wide swathes of research and experimental applications.

Our polyclonal antibodies can easily support your work, whether you use them for Western Blotting, Immunocytochemistry (with or without Immunofluorescence used in conjunction), Immunohistochemistry, Immunoprecipitation, and ELISA tests. We highly encourage you to browse our range of pAbs and choose the one that best suits your experimental model.

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human liver cancer tissue using BMPR1B Polyclonal Antibody at dilution 1:25)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using BMPR1B Polyclonal Antibody at dilution 1:25)

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BMPR1B, Polyclonal Antibody (Cat# AAA169247)

Full Name

BMPR1B Polyclonal Antibody

Gene Names

BMPR1B; ALK6; ALK-6; CDw293

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Purity

Antigen affinity purification

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human gastric cancer using ACTA2 Polyclonal Antibody at dilution of 1:40)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Mouse kidney using ACTA2 Polyclonal Antibody at dilution of 1:50)

WB (Western Blot) (Western Blot analysis of Mouse heart tissue using ACTA2 Polyclonal Antibody at dilution of 1:800)

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ACTA2, Polyclonal Antibody (Cat# AAA169261)

Full Name

ACTA2 Polyclonal Antibody

Gene Names

ACTA2; AAT6; ACTSA; MYMY5

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen affinity purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemical of paraffin-embedded human thyroid cancer using HAX1 antibody at dilution of 1:200 (400x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines, using HAX1 antibody.)

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HAX1, Polyclonal Antibody (Cat# AAA169272)

Full Name

HAX1 Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen affinity purification

Pricing

$305/0.06 mL $420/0.12 mL $650/0.2 mL $1,260/2x0.2 mL $1,845/3x0.2 mL $2,390/4x0.2 mL $2,880/5x0.2 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human tonsil tissue using GPCPD1 Polyclonal Antibody at dilution of 1:50(×200))

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using GPCPD1 Polyclonal Antibody at dilution of 1:50(×200))

WB (Western Blot) (Western blot analysis of K562 cell Mouse liver tissue lysates using GPCPD1 Polyclonal Antibody at dilution of 1:650)

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GPCPD1, Polyclonal Antibody (Cat# AAA176624)

Full Name

GPCPD1 Polyclonal Antibody

Gene Names

GPCPD1; EDI3; GDE5; GDPD6; PREI4

Reactivity

Human, Monkey, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human tonsil tissue using TXNDC12 Polyclonal Antibody at dilution of 1:50(×200))

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human brain tissue using TXNDC12 Polyclonal Antibody at dilution of 1:50(×200))

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TXNDC12, Polyclonal Antibody (Cat# AAA176517)

Full Name

TXNDC12 Polyclonal Antibody

Gene Names

TXNDC12; AG1; AGR1; ERP16; ERP18; ERP19; TLP19; hAG-1; PDIA16; hTLP19

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Purity

Antigen Affinity Purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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WB (Western Blot) (WB Suggested Anti-CEMP1 AntibodyTitration: 1.0 ug/mlPositive Control: Hela Whole cell)

WB (Western Blot) (Host: RabbitTarget Name: CEMP1Sample Tissue: Human HepG2 Whole CellAntibody Dilution: 1ug/ml)

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CEMP1, Polyclonal Antibody (Cat# AAA201366)

Full Name

CEMP1 Antibody - C-terminal region

Gene Names

CEMP1; CP23; CP-23

Reactivity

Human

Applications

Western Blot

Purity

Affinity Purified

Pricing

$500/0.1 mL $975/2x0.1 mL $1,430/3x0.1 mL $1,855/4x0.1 mL $2,115/5x0.1 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human lung cancer using IGFBP7 Polyclonal Antibody at dilution of 1:25)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human breast cancer using IGFBP7 Polyclonal Antibody at dilution of 1:25)

WB (Western Blot) (Western Blot analysis of Mouse brain tissue using IGFBP7 Polyclonal Antibody at dilution of 1:400)

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IGFBP7, Polyclonal Antibody (Cat# AAA167755)

Full Name

IGFBP7 Polyclonal Antibody

Gene Names

IGFBP7; AGM; PSF; TAF; FSTL2; IBP-7; MAC25; IGFBP-7; RAMSVPS; IGFBP-7v; IGFBPRP1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen affinity purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human liver cancer tissue using DSTN Polyclonal Antibody at dilution 1:30)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human thyroid cancer tissue using DSTN Polyclonal Antibody at dilution 1:30)

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DSTN, Polyclonal Antibody (Cat# AAA168033)

Full Name

DSTN Polyclonal Antibody

Gene Names

DSTN; ADF; ACTDP; HEL32; bA462D18.2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Purity

Antigen affinity purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human breast cancer tissue using DDX11 Polyclonal Antibody at dilution 1:30)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human ovarian cancer tissue using DDX11 Polyclonal Antibody at dilution 1:30)

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DDX11, Polyclonal Antibody (Cat# AAA168105)

Full Name

DDX11 Polyclonal Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Purity

Antigen affinity purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human colon cancer using CASP10 Polyclonal Antibody at dilution of 1:20)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human brain using CASP10 Polyclonal Antibody at dilution of 1:20)

WB (Western Blot) (Western Blot analysis of Human liver cancer tissue using CASP10 Polyclonal Antibody at dilution of 1:300)

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CASP10, Polyclonal Antibody (Cat# AAA167311)

Full Name

CASP10 Polyclonal Antibody

Gene Names

CASP10; MCH4; ALPS2; FLICE2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen affinity purification

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human breast cancer using ARHGDIA Polyclonal Antibody at dilution of 1:30)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human liver cancer using ARHGDIA Polyclonal Antibody at dilution of 1:30)

WB (Western Blot) (Western Blot analysis of Mouse spleen tissue, A375, A549, HT-29 and Hela cell using ARHGDIA Polyclonal Antibody at dilution of 1:350)

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ARHGDIA, Polyclonal Antibody (Cat# AAA170157)

Full Name

ARHGDIA Polyclonal Antibody

Gene Names

ARHGDIA; GDIA1; NPHS8; RHOGDI; RHOGDI-1; HEL-S-47e

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen affinity purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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WB (Western Blot) (Western Blot analysis of Rat kidney using Phospho-KOR-1 (Ser369) Polyclonal Antibody at dilution of 1:1000.)

WB (Western Blot) (Western Blot analysis of KB, 22RV1 cells using Phospho-KOR-1 (Ser369) Polyclonal Antibody at dilution of 1:1000.)

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KOR-1, Polyclonal Antibody (Cat# AAA172268)

Full Name

Phospho-KOR-1 (S369) Polyclonal Antibody

Reactivity

Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purified

Pricing

$180/0.02 mL $230/0.06 mL $345/0.12 mL $510/0.2 mL $995/2x0.2 mL $1,455/3x0.2 mL $1,890/4x0.2 mL $2,140/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human tonsil tissue using CEP89 Polyclonal Antibody at dilution of 1:95(×200))

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human brain tissue using CEP89 Polyclonal Antibody at dilution of 1:95(×200))

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CEP89, Polyclonal Antibody (Cat# AAA175649)

Full Name

CEP89 Polyclonal Antibody

Gene Names

CEP89; CEP123; CCDC123

Reactivity

Human

Applications

Immunohistochemistry

Purity

Antigen Affinity Purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using MBL2 Polyclonal Antibody at dilution of 1:25(×200))

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human colorectal cancer tissue using MBL2 Polyclonal Antibody at dilution of 1:25(×200))

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MBL2, Polyclonal Antibody (Cat# AAA175797)

Full Name

MBL2 Polyclonal Antibody

Gene Names

MBL2; MBL; MBP; MBP1; MBPD; MBL2D; MBP-C; COLEC1; HSMBPC

Reactivity

Human, Rat

Applications

Immunohistochemistry

Purity

Antigen Affinity Purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human tonsil tissue using LRAT Polyclonal Antibody at dilution of 1:55(×200))

WB (Western Blot) (Western blot analysis of SP20 cell lysate using LRAT Polyclonal Antibody at dilution of 1:800)

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LRAT, Polyclonal Antibody (Cat# AAA175611)

Full Name

LRAT Polyclonal Antibody

Gene Names

LRAT; LCA14

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purification

Pricing

$170/0.02 mL $235/0.06 mL $340/0.12 mL $495/0.2 mL $965/2x0.2 mL $1,415/3x0.2 mL $1,835/4x0.2 mL $2,205/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of Rat heart using LAMC1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of Rat brain using LAMC1 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human uterine cancer using LAMC1 Polyclonal Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human uterus using LAMC1 Polyclonal Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human breast cancer using LAMC1 Polyclonal Antibody at dilution of 1:100 (40x lens).)

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LAMC1, Polyclonal Antibody (Cat# AAA177328)

Full Name

LAMC1 Polyclonal Antibody

Gene Names

LAMC1; LAMB2

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry

Purity

Affinity purification

Pricing

$305/0.06 mL $420/0.12 mL $650/0.2 mL $1,260/2x0.2 mL $1,845/3x0.2 mL $2,390/4x0.2 mL $2,880/5x0.2 mL

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Mouse kidney using SMN1 Polyclonal Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human breast using SMN1 Polyclonal Antibody at dilution of 1:100 (40x lens).)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Rat lung using SMN1 Polyclonal Antibody at dilution of 1:100 (40x lens).)

WB (Western Blot) (Western blot analysis of extracts of various cell lines using SMN1 Polyclonal Antibody at dilution of 1:1000.)

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SMN1, Polyclonal Antibody (Cat# AAA177353)

Full Name

SMN1 Polyclonal Antibody

Gene Names

SMN1; SMA; SMN; SMA1; SMA2; SMA3; SMA4; SMA@; SMNT; BCD541; GEMIN1; TDRD16A; T-BCD541

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity purification

Pricing

$230/0.06 mL $340/0.12 mL $505/0.2 mL $985/2x0.2 mL $1,440/3x0.2 mL $1,875/4x0.2 mL $2,125/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U-2 OS cells using CD47 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of L929 cells using CD47 Polyclonal Antibody at dilution of 1:100. Blue: DAPI for nuclear staining.)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human placenta using CD47 Polyclonal Antibody at dilution of 1:200 (40x lens).)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded Human lung cancer using CD47 Polyclonal Antibody at dilution of 1:200 (40x lens).)

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CD47, Polyclonal Antibody (Cat# AAA177562)

Full Name

CD47 Polyclonal Antibody

Gene Names

CD47; IAP; OA3; MER6

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry

Purity

Affinity purification

Pricing

$300/0.06 mL $410/0.12 mL $630/0.2 mL $1,220/2x0.2 mL $1,780/3x0.2 mL $2,315/4x0.2 mL $2,830/5x0.2 mL

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WB (Western Blot) (Western blotting of Dimethyl-Lysine polyclonal antibody at dilution of 1:500.)

DB (Dot Blot) (Dot blotting analysis of anti-Dimethyl-Lysine polyclonal antibody on unmodified BSA and dimethyl BSA at dilution of 1:2000.)

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Dimethyl-Lysine, Polyclonal Antibody (Cat# AAA178652)

Full Name

Dimethyl-Lysine Polyclonal Antibody

Reactivity

Human, Mouse, E coli

Applications

Dot Blot, Western Blot

Purity

Antigen Affinity Purification

Pricing

$195/0.025 mL $355/0.1 mL $685/2x0.1 mL $995/3x0.1 mL $1,295/4x0.1 mL $1,560/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts of various cell lines using SNRNP40 Polyclonal Antibody at 1:1000 dilution.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines using SNRNP40 Polyclonal Antibody at 1:1000 dilution.)

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SNRNP40, Polyclonal Antibody (Cat# AAA179207)

Full Name

SNRNP40 Polyclonal Antibody

Gene Names

SNRNP40; 40K; SPF38; WDR57; PRP8BP; HPRP8BP; PRPF8BP

Reactivity

Human, Mouse

Applications

Western Blot

Purity

Affinity purification

Pricing

$300/0.06 mL $410/0.12 mL $630/0.2 mL $1,220/2x0.2 mL $1,780/3x0.2 mL $2,315/4x0.2 mL $2,830/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded mouse adrenal gland using AGRP Polyclonal Antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of extracts of Mouse skeletal muscle using AGRP Polyclonal Antibody at 1:1000 dilution.)

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AGRP, Polyclonal Antibody (Cat# AAA179446)

Full Name

AGRP Polyclonal Antibody

Gene Names

AGRP; ART; AGRT; ASIP2

Reactivity

Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity purification

Pricing

$305/0.06 mL $420/0.12 mL $650/0.2 mL $1,260/2x0.2 mL $1,845/3x0.2 mL $2,390/4x0.2 mL $2,880/5x0.2 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded human placenta using AAA118289 at dilution 1:100)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded human rectum using AAA118289 at dilution 1:100)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human prostate using AAA118289 at dilution 1:100)

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BMP and activin membrane-bound inhibitor homolog, Polyclonal Antibody (Cat# AAA118289)

Full Name

Rabbit anti-human BMP and activin membrane-bound inhibitor homolog polyclonal Antibody(BAMBI)

Gene Names

BAMBI; NMA

Reactivity

Human

Applications

Immunohistochemistry

Purity

Antigen Affinity Purified

Pricing

$235/0.05 mL $345/0.1 mL $665/2x0.1 mL $965/3x0.1 mL $1,255/4x0.1 mL $1,490/5x0.1 mL

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WB (Western Blot) (Western BlotPositive WB detected in:HepG2 whole cell lysate,HEK293 whole cell lysate,Mouse kidney tissueAll lanes: GEMIN8 antibody at 4ug/mlSecondaryGoat polyclonal to rabbit IgG at 1/50000 dilutionPredicted band size: 29 kDaObserved band size: 29,33 kDa)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human adrenal gland tissue using AAA118416 at dilution of 1:100)

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GEMIN8, Polyclonal Antibody (Cat# AAA118416)

Full Name

GEMIN8 Antibody

Gene Names

GEMIN8; FAM51A1

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

>95%, Protein G Purified

Pricing

$235/0.05 mg $345/0.1 mg $665/2x0.1 mg $965/3x0.1 mg $1,255/4x0.1 mg $1,490/5x0.1 mg

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IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human lung using AGER Polyclonal Antibody at dilution of 1:450.)

WB (Western Blot) (Western blot with Anti AGER Polyclonal antibody at dilution of 1:5000. Lane 1: Mouse lung tissue lysate, Lane 2: Rat lung tissue lysate.)

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AGER, Polyclonal Antibody (Cat# AAA179749)

Full Name

AGER Polyclonal antibody

Gene Names

AGER; RAGE

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purification

Pricing

$195/0.025 mL $355/0.1 mL $685/2x0.1 mL $995/3x0.1 mL $1,295/4x0.1 mL $1,560/5x0.1 mL

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IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded Human breast cancer using Adipoq Polyclonal Antibody at dilution of 1:600)

IHC (Immunohiostchemistry) (Immunohistochemistry of paraffin-embedded Human lung using Adipoq Polyclonal Antibody at dilution of 1:600)

WB (Western Blot) (Western blot with Adipoq Polyclonal antibody at dilution of 1:500.lane 1:Mouse heart, lane 2:Rat serum)

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ADIPOQ, Polyclonal Antibody (Cat# AAA179763)

Full Name

ADIPOQ Polyclonal Antibody

Gene Names

Adipoq; Acdc; Acrp30

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antigen Affinity Purification

Pricing

$195/0.025 mL $355/0.1 mL $685/2x0.1 mL $995/3x0.1 mL $1,295/4x0.1 mL $1,560/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded rat ovary using MTAP Polyclonal antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded mouse kidney using MTAP Polyclonal antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

IHC (Immunohistochemisry) (Immunohistochemistry of paraffin-embedded human colon carcinoma using MTAP Polyclonal antibody at dilution of 1:50 (40x lens).Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines using MTAP Polyclonal Antibody at 1:1000 dilution.)

WB (Western Blot) (Western blot analysis of extracts of NIH/3T3 cells using MTAP Polyclonal Antibody at 1:1000 dilution.)

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MTAP, Polyclonal Antibody (Cat# AAA178676)

Full Name

MTAP Polyclonal Antibody

Gene Names

MTAP; BDMF; MSAP; DMSFH; LGMBF; DMSMFH; c86fus; HEL-249

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity purification

Pricing

$305/0.06 mL $420/0.12 mL $650/0.2 mL $1,260/2x0.2 mL $1,845/3x0.2 mL $2,390/4x0.2 mL $2,880/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells using HMGCR Polyclonal Antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells using HMGCR Polyclonal Antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.)

IF (Immunofluorescence) (Immunofluorescence analysis of A-549 cells using HMGCR Polyclonal Antibody at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of various lysates using HMGCR Polyclonal Antibody at 1:1000 dilution.)

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HMGCR, Polyclonal Antibody (Cat# AAA179577)

Full Name

HMGCR Polyclonal Antibody

Gene Names

HMGCR; LDLCQ3

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Affinity purification

Pricing

$305/0.06 mL $420/0.12 mL $650/0.2 mL $1,260/2x0.2 mL $1,845/3x0.2 mL $2,390/4x0.2 mL $2,880/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of U2OS cells using JAK1 Polyclonal Antibody at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.)

WB (Western Blot) (Western blot analysis of extracts of Mouse lung using JAK1 Polyclonal Antibody at 1:1000 dilution.)

WB (Western Blot) (Western blot analysis of extracts of various cell lines using JAK1 Polyclonal Antibody at 1:1000 dilution.)

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JAK1, Polyclonal Antibody (Cat# AAA179541)

Full Name

JAK1 Polyclonal Antibody

Gene Names

JAK1; JTK3; JAK1A; JAK1B

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Affinity purification

Pricing

$300/0.06 mL $410/0.12 mL $630/0.2 mL $1,220/2x0.2 mL $1,780/3x0.2 mL $2,315/4x0.2 mL $2,830/5x0.2 mL

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FCM/FACS (Flow Cytometry) (Figure 3. Flow Cytometry analysis of ANA-1 cells using anti-Sox11 antibody (AAA126238).Overlay histogram showing ANA-1 cells stained with AAA126238 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-Sox11 Antibody (AAA126238, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IHC (Immunohiostchemistry) (Figure 2. IHC analysis of Sox11 using anti-Sox11 antibody (AAA126238).Sox11 was detected in a paraffin-embedded section of mouse pancreas tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2 ug/ml rabbit anti-Sox11 Antibody (AAA126238) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of Sox11 using anti-Sox11 antibody (AAA126238).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: rat C6 whole cell lysates,Lane 2: mouse SP2/0 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-Sox11 antigen affinity purified polyclonal antibody (#AAA126238) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for Sox11 at approximately 55 kDa. The expected band size for Sox11 is at 55 kDa.)

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Sox11, Polyclonal Antibody (Cat# AAA126238)

Full Name

Anti-Sox11 Antibody Picoband

Gene Names

Sox11; end1; AI836553; 1110038H03Rik; 6230403H02Rik

Reactivity

Mouse, Rat

Applications

Flow Cytometry, Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

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FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of HEL cells using anti-CXCR5 antibody (AAA126054).Overlay histogram showing HEL cells stained with AAA126054 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-CXCR5 Antibody (AAA126054, 1 ug/1x10^6 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1 ug/1x10^6) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

WB (Western Blot) (Figure 1. Western blot analysis of CXCR5 using anti-CXCR5 antibody (AAA126054).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Raji whole cell lysates,Lane 2: human MOLT-4 whole cell lysates,Lane 3: human Hela whole cell lysates,Lane 4: rat brain tissue lysates,Lane 5: mouse brain tissue lysates,Lane 6: mouse lung tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-CXCR5 antigen affinity purified polyclonal antibody (#AAA126054) at 0.5 ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for CXCR5 at approximately 50 kDa. The expected band size for CXCR5 is at 42 kDa.)

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CXCR5, Polyclonal Antibody (Cat# AAA126054)

Full Name

Anti-CXCR5 Antibody Picoband

Gene Names

CXCR5; BLR1; CD185; MDR15

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg (Carrier Free) $485/0.1 mg $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Cy3) $705/0.1 mg (Dylight488) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (PE) $810/0.1 mg (iFluor647) $2,005/5x0.1 mg

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FCM/FACS (Flow Cytometry) (Figure 3. Flow Cytometry analysis of K562 cells using anti-SYCN antibody (AAA127914).Overlay histogram showing K562 cells stained with AAA127914 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SYCN Antibody (AAA127914, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 2. IF analysis of SYCN using anti-SYCN antibody (AAA127914).SYCN was detected in an immunocytochemical section of PC-3 cells. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-SYCN Antibody (AAA127914) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) was used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

WB (Western Blot) (Figure 1. Western blot analysis of SYCN using anti-SYCN antibody (AAA127914).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: rat pancreas tissue lysates,Lane 2: mouse pancreas tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYCN antigen affinity purified polyclonal antibody (#AAA127914) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SYCN at approximately 14 kDa. The expected band size for SYCN is at 14 kDa.)

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SYCN, Polyclonal Antibody (Cat# AAA127914)

Full Name

Anti-SYCN Antibody Picoband

Gene Names

SYCN; SYL; INSSA1

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

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FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of SiHa cells using anti-FOXN1 antibody (AAA125691).Overlay histogram showing SiHa cells stained with AAA125691 (Blue line). The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-FOXN1 Antibody (AAA125691, 1μg/1x106 cells) for 30 min at 20 degree C. DyLight®488 conjugated goat anti-rabbit IgG (5-10μg/1x106 cells) was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1μg/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

WB (Western Blot) (Figure 1. Western blot analysis of FOXN1 using anti-FOXN1 antibody (AAA125691).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30ug of sample under reducing conditions.Lane 1: human Hela whole cell lysatesLane 2: human Raji whole cell lysatesLane 3: mouse NIH/3T3 whole cell lysatesLane 4: mouse RAW264. 7 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1. 5 hour at RT. The membrane was incubated with rabbit anti-FOXN1 antigen affinity purified polyclonal antibody (Catalog # AAA125691) at 0. 5 μg/mL overnight at 4 degree C, then washed with TBS-0. 1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1. 5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for FOXN1 at approximately 69KD. The expected band size for FOXN1 is at 69KD.)

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FOXN1, Polyclonal Antibody (Cat# AAA125691)

Full Name

Anti-FOXN1 Antibody

Gene Names

FOXN1; WHN; RONU; FKHL20

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

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FCM/FACS (Flow Cytometry) (Figure 3. Flow Cytometry analysis of U20S cells using anti-RANBP9 antibody (AAA127176).Overlay histogram showing U20S cells stained with AAA127176 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RANBP9 Antibody (AAA127176, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 2. IF analysis of RANBP9 using anti-RANBP9 antibody (AAA127176) and anti-Beta Tubulin antibody (M01857-3).RANBP9 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-RANBP9 Antibody (AAA127176) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG and Cy3 Conjugated Goat Anti-Mouse IgG (BA1031) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. The section was counterstained with DAPI. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

WB (Western Blot) (Figure 1. Western blot analysis of RANBP9 using anti-RANBP9 antibody (AAA127176).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human Jurkat whole cell lysates,Lane 3: human CCRF-CEM whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RANBP9 antigen affinity purified polyclonal antibody (#AAA127176) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RANBP9 at approximately 91 kDa. The expected band size for RANBP9 is at 78 kDa.)

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RANBP9, Polyclonal Antibody (Cat# AAA127176)

Full Name

Anti-RANBP9 Antibody Picoband

Gene Names

RANBP9; BPM-L; BPM90; RANBPM; RanBP7

Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $705/0.1 mg (Dylight594) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $810/0.1 mg (APC) $2,005/5x0.1 mg

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FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of K562 cells using anti-SYNGAP1 antibody (AAA127260).Overlay histogram showing K562 cells stained with AAA127260 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-SYNGAP1 Antibody (AAA127260, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

WB (Western Blot) (Figure 1. Western blot analysis of SYNGAP1 using anti-SYNGAP1 antibody (AAA127260).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: rat brain tissue lysates,Lane 2: mouse brain tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SYNGAP1 antigen affinity purified polyclonal antibody (#AAA127260) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SYNGAP1 at approximately 148 kDa. The expected band size for SYNGAP1 is at 148 kDa.)

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SYNGAP1, Polyclonal Antibody (Cat# AAA127260)

Full Name

Anti-SYNGAP1 Antibody Picoband

Gene Names

SYNGAP1; MRD5; RASA1; RASA5; SYNGAP

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg (Carrier Free) $485/0.1 mg $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Cy3) $705/0.1 mg (Dylight488) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (PE) $810/0.1 mg (iFluor647) $2,005/5x0.1 mg

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FCM/FACS (Flow Cytometry) (Figure 5. Flow Cytometry analysis of SH-SY5Y cells using anti-RNF111 antibody (AAA127320).Overlay histogram showing SH-SY5Y cells stained with AAA127320 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-RNF111 Antibody (AAA127320, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

IF (Immunofluorescence) (Figure 4. IF analysis of RNF111 using anti-RNF111 antibody (AAA127320) and anti-Beta Tubulin antibody (M01857-3).RNF111 was detected in immunocytochemical section of A549 cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-RNF111 Antibody (AAA127320) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4 degree C. DyLight488 Conjugated Goat Anti-Rabbit IgG and DyLight550 Conjugated Goat Anti-Mouse IgG (BA1133) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

IHC (Immunohistochemisry) (Figure 3. IHC analysis of RNF111 using anti-RNF111 antibody (AAA127320).RNF111 was detected in a paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RNF111 Antibody (AAA127320) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

IHC (Immunohiostchemistry) (Figure 2. IHC analysis of RNF111 using anti-RNF111 antibody (AAA127320).RNF111 was detected in a paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in EDTA buffer (pH 8.0, epitope retrieval solution). The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-RNF111 Antibody (AAA127320) overnight at 4 degree C. Peroxidase Conjugated Goat Anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using HRP Conjugated Rabbit IgG Super Vision Assay Kit with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of RNF111 using anti-RNF111 antibody (AAA127320).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: mouse thymus tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-RNF111 antigen affinity purified polyclonal antibody (#AAA127320) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for RNF111 at approximately 109 kDa. The expected band size for RNF111 is at 109 kDa.)

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RNF111, Polyclonal Antibody (Cat# AAA127320)

Full Name

Anti-RNF111 Antibody Picoband

Gene Names

RNF111; ARK

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Immunofluorescence, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (HRP) $705/0.1 mg (FITC) $705/0.1 mg (Cy3) $705/0.1 mg (Dylight488) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight594) $705/0.1 mg (Biotin) $810/0.1 mg (PE) $810/0.1 mg (iFluor647) $810/0.1 mg (APC) $2,005/5x0.1 mg

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FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of JK cells using anti-PET112/GATB antibody (AAA127497).Overlay histogram showing JK cells stained with AAA127497 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-PET112/GATB Antibody (AAA127497, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

WB (Western Blot) (Figure 1. Western blot analysis of PET112/GATB using anti-PET112/GATB antibody (AAA127497).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human HepG2 whole cell lysates,Lane 3: human Jurkat whole cell lysates,Lane 4: human PC-3 whole cell lysates,Lane 5: mouse kidney tissue lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-PET112/GATB antigen affinity purified polyclonal antibody (#AAA127497) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for PET112/GATB at approximately 62 kDa. The expected band size for PET112/GATB is at 62 kDa.)

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GATB, Polyclonal Antibody (Cat# AAA127497)

Full Name

Anti-GATB Antibody Picoband

Gene Names

GATB; PET112; HSPC199; PET112L

Reactivity

Human, Mouse

Applications

Flow Cytometry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

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FCM/FACS (Flow Cytometry) (Figure 3. Flow Cytometry analysis of HepG2 cells using anti-MPHOSPH10 antibody (AAA127715).Overlay histogram showing HepG2 cells stained with AAA127715 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-MPHOSPH10 Antibody (AAA127715, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample (Red line) was also used as a control.)

IF (Immunofluorescence) (Figure 2. IF analysis of MPHOSPH10 using anti-MPHOSPH10 antibody (AAA127715) and anti-Beta Tubulin antibody (M01857-3).MPHOSPH10 was detected in immunocytochemical section of HELA cell. Enzyme antigen retrieval was performed using IHC enzyme antigen retrieval reagent for 15 mins. The cells were blocked with 10% goat serum. And then incubated with 5ug/mL rabbit anti-MPHOSPH10 Antibody (AAA127715) and mouse anti-Beta Tubulin antibody (M01857-3) overnight at 4 degree C. Cy3 Conjugated Goat Anti-Rabbit IgG (BA1032) and DyLight488 Conjugated Goat Anti-Mouse IgG (BA1126) were used as secondary antibody at 1:500 dilution and incubated for 30 minutes at 37 degree C. Visualize using a fluorescence microscope and filter sets appropriate for the label used.)

WB (Western Blot) (Figure 1. Western blot analysis of MPHOSPH10 using anti-MPHOSPH10 antibody (AAA127715).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human Hela whole cell lysates,Lane 2: human HepG2 whole cell lysates,Lane 3: human K562 whole cell lysates,Lane 4: human HEL whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-MPHOSPH10 antigen affinity purified polyclonal antibody (#AAA127715) at 0.25ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for MPHOSPH10 at approximately 120 kDa. The expected band size for MPHOSPH10 is at 120 kDa.)

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MPHOSPH10, Polyclonal Antibody (Cat# AAA127715)

Full Name

Anti-MPHOSPH10 Antibody Picoband

Gene Names

MPHOSPH10; CT90; MPP10; MPP10P; PPP1R106

Reactivity

Human

Applications

Flow Cytometry, Immunofluorescence, Immunocytochemistry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight488) $705/0.1 mg (Cy3) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (iFluor647) $810/0.1 mg (PE) $2,005/5x0.1 mg

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IHC (Immunohistochemistry) (Figure 4. IHC analysis of SHIP using anti-SHIP antibody (AAA124705).SHIP was detected in paraffin-embedded section of rat spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SHIP Antibody (AAA124705) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohistochemisry) (Figure 3. IHC analysis of SHIP using anti-SHIP antibody (AAA124705).SHIP was detected in paraffin-embedded section of mouse spleen tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SHIP Antibody (AAA124705) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

IHC (Immunohiostchemistry) (Figure 2. IHC analysis of SHIP using anti-SHIP antibody (AAA124705).SHIP was detected in paraffin-embedded section of human tonsil tissue. Heat mediated antigen retrieval was performed in citrate buffer (pH6, epitope retrieval solution) for 20 mins. The tissue section was blocked with 10% goat serum. The tissue section was then incubated with 2ug/ml rabbit anti-SHIP Antibody (AAA124705) overnight at 4 degree C. Biotinylated goat anti-rabbit IgG was used as secondary antibody and incubated for 30 minutes at 37 degree C. The tissue section was developed using Strepavidin-Biotin-Complex (SABC) with DAB as the chromogen.)

WB (Western Blot) (Figure 1. Western blot analysis of SHIP using anti-SHIP antibody (AAA124705).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel) / 90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 50ug of sample under reducing conditions.Lane 1: human CCRF-CEM whole cell lysates,Lane 2: human SW620 whole cell lysates.After Electrophoresis, proteins were transferred to a Nitrocellulose membrane at 150mA for 50-90 minutes. Blocked the membrane with 5% Non-fat Milk/ TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-SHIP antigen affinity purified polyclonal antibody at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:10000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for SHIP at approximately 145KD. The expected band size for SHIP is at 133KD.)

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SHIP, Polyclonal Antibody (Cat# AAA124705)

Full Name

Anti-SHIP Picoband Antibody

Gene Names

INPP5D; SHIP; SHIP1; SHIP-1; hp51CN; SIP-145; p150Ship

Reactivity

Human, Mouse, Rat
No cross reactivity with other proteins.

Applications

Western Blot, Immunohistochemistry

Pricing

$215/0.01 mg $485/0.1 mg (Carrier Free) $485/0.1 mg $705/0.1 mg (Dylight594) $705/0.1 mg (Dylight550) $705/0.1 mg (Cy3) $705/0.1 mg (Dylight488) $705/0.1 mg (FITC) $705/0.1 mg (HRP) $705/0.1 mg (Biotin) $810/0.1 mg (APC) $810/0.1 mg (PE) $810/0.1 mg (iFluor647) $2,005/5x0.1 mg

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FCM/FACS (Flow Cytometry) (Figure 2. Flow Cytometry analysis of MCF-7 cells using anti-EGR2 antibody (AAA127019).Overlay histogram showing MCF-7 cells stained with AAA127019 (Blue line). To facilitate intracellular staining, cells were fixed with 4% paraformaldehyde and permeabilized with permeabilization buffer. The cells were blocked with 10% normal goat serum. And then incubated with rabbit anti-EGR2 Antibody (AAA127019, 1ug/1x106 cells) for 30 min at 20 degree C. DyLight488 conjugated goat anti-rabbit IgG was used as secondary antibody for 30 minutes at 20 degree C. Isotype control antibody (Green line) was rabbit IgG (1ug/1x106) used under the same conditions. Unlabelled sample without incubation with primary antibody and secondary antibody (Red line) was used as a blank control.)

WB (Western Blot) (Figure 1. Western blot analysis of EGR2 using anti-EGR2 antibody (AAA127019).Electrophoresis was performed on a 5-20% SDS-PAGE gel at 70V (Stacking gel)/90V (Resolving gel) for 2-3 hours. The sample well of each lane was loaded with 30 ug of sample under reducing conditions.Lane 1: human PC-3 whole cell lysates,Lane 2: human U251 whole cell lysates,Lane 3: human U20S whole cell lysates,Lane 4: human MCF-7 whole cell lysates,Lane 5: rat thymus tissue lysates,Lane 6: rat PC-12 whole cell lysates,Lane 7: mouse thymus tissue lysates,Lane 8: mouse Raw264.7 whole cell lysates.After electrophoresis, proteins were transferred to a nitrocellulose membrane at 150 mA for 50-90 minutes. Blocked the membrane with 5% non-fat milk/TBS for 1.5 hour at RT. The membrane was incubated with rabbit anti-EGR2 antigen affinity purified polyclonal antibody (#AAA127019) at 0.5ug/mL overnight at 4 degree C, then washed with TBS-0.1%Tween 3 times with 5 minutes each and probed with a goat anti-rabbit IgG-HRP secondary antibody at a dilution of 1:5000 for 1.5 hour at RT. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit with Tanon 5200 system. A specific band was detected for EGR2 at approximately 50 kDa. The expected band size for EGR2 is at 50,45 kDa.)

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EGR2, Polyclonal Antibody (Cat# AAA127019)

Full Name

Anti-EGR2 Antibody Picoband

Gene Names

EGR2; AT591; CMT1D; CMT4E; KROX20

Reactivity

Human, Mouse, Rat

Applications

Flow Cytometry, Western Blot

Purity

Immunogen affinity purified.

Pricing

$215/0.01 mg $485/0.1 mg $485/0.1 mg (Carrier Free) $705/0.1 mg (Biotin) $705/0.1 mg (HRP) $705/0.1 mg (FITC) $705/0.1 mg (Cy3) $705/0.1 mg (Dylight488) $705/0.1 mg (Dylight550) $705/0.1 mg (Dylight594) $810/0.1 mg (PE) $810/0.1 mg (iFluor647) $810/0.1 mg (APC) $2,005/5x0.1 mg

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WB (Western Blot) (Antibody (0.2 ug/ml) staining of HEK293 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

IHC (Immunohiostchemistry) (Anti-P4HA1 antibody IHC of human spleen. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

IHC (Immunohistochemistry) (Anti-P4HA1 antibody IHC of human skin. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

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P4HA1, Polyclonal Antibody (Cat# AAA50914)

Full Name

Goat Polyclonal to Human P4HA1

Gene Names

P4HA1; P4HA

Reactivity

Human, Monkey, Bat, Bovine, Dog, Horse, Pig, Rabbit, Chicken (tested or 100% immunogen sequence identity)

Applications

Western Blot, Immunohistochemistry

Purity

Immunoaffinity Purified

Pricing

$695/0.05 mg $1,355/2x0.05 mg $1,975/3x0.05 mg $2,565/4x0.05 mg $2,920/5x0.05 mg

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WB (Western Blot) (Western blot of ACE2 in human kidney lysate with ACE2 antibody at (A) 0.5, (B) 1 and (C) 2 ug/ml.)

IF (Immunofluorescence) (Immunofluorescence of ACE2 in Human Kidney cells with ACE2 antibody at 20 ug/ml.)

IHC (Immunohistochemistry) (Anti-ACE-2 antibody IHC of human kidney. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

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ACE2 / ACE-2, Polyclonal Antibody (Cat# AAA50734)

Full Name

Rabbit Polyclonal to Human ACE2 / ACE-2

Gene Names

ACE2; ACEH

Reactivity

Human, Mouse

Applications

Western Blot, Immunohistochemistry

Purity

Immunoaffinity Purified

Pricing

$765/0.05 mg $1,495/2x0.05 mg $2,185/3x0.05 mg $2,835/4x0.05 mg $3,235/5x0.05 mg

IHC (Immunohistochemistry) (Anti-S1PR1 / EDG1 antibody IHC of human placenta, endothelium. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 2.5 ug/ml.)

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S1PR1 / EDG1 / S1P1, Polyclonal Antibody (Cat# AAA51492)

Full Name

Rabbit Polyclonal (IgG) to Human S1PR1 / EDG1 / S1P1

Gene Names

S1PR1; EDG1; S1P1; CD363; ECGF1; EDG-1; CHEDG1; D1S3362

Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunohistochemistry

Purity

Affinity Purified

Pricing

$665/0.05 mL $1,295/2x0.05 mL $1,890/3x0.05 mL $2,450/4x0.05 mL $2,775/5x0.05 mL

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WB (Western Blot) (OAS2 antibody (0.3 ug/ml) staining of Daudi (A), Jurkat (B) and K562 (C) lysates (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.)

IHC (Immunohistochemistry) (Anti-OAS2 antibody IHC staining of human breast. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

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OAS2, Polyclonal Antibody (Cat# AAA52473)

Full Name

Goat Polyclonal to Human OAS2

Reactivity

Gibbon, Chimpanzee, Human

Applications

Western Blot, Immunohistochemistry

Purity

Immunoaffinity Purified

Pricing

$695/0.05 mg $1,355/2x0.05 mg $1,975/3x0.05 mg $2,565/4x0.05 mg $2,920/5x0.05 mg

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IHC (Immunohistochemistry) (DAB staining on IHC-P; Samples: Human Skin cancer Tissue))

IHC (Immunohistochemisry) (DAB staining on IHC-P; Samples: Human Skin Cancer Tissue)

WB (Western Blot) (Western Blot: Sample: Recombinant S100A14, Human.)

WB (Western Blot) (Western Blot: Sample: Porcine Stomach Tissue.)

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S100 Calcium Binding Protein A14, Polyclonal Antibody (Cat# AAA142669)

Full Name

Polyclonal Antibody to S100 Calcium Binding Protein A14 (S100A14)

Gene Names

S100A14; BCMP84; S100A15

Reactivity

Human, Mouse, Pig

Applications

Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Affinity Chromatography

Pricing

$220/0.1 mL $280/0.2 mL $440/0.5 mL $540/1 mL $1,455/5 mL $2,835/2x5 mL $4,145/3x5 mL $5,375/4x5 mL $6,545/5x5 mL

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IHC (Immunohiostchemistry) (DAB staining on IHC-P;Samples: Porcine Stomach Tissue;Primary Ab: 20ug/ml Rabbit Anti-Porcine MCP1 AntibodySecond Ab: 2ug/mL HRPLinked Caprine Anti-Rabbit IgG Polyclonal Antibody)

WB (Western Blot) (Western Blot: Sample: Recombinant MCP1, Porcine.)

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Monocyte Chemotactic Protein 1, Polyclonal Antibody (Cat# AAA142719)

Full Name

Polyclonal Antibody to Monocyte Chemotactic Protein 1 (MCP1)

Gene Names

CCL2; MCP-1

Reactivity

Pig

Applications

Immunoprecipitation, Immunocytochemistry, Immunohistochemistry, Western Blot

Purity

Antigen-specific affinity chromatography followed by Protein A affinity chromatography

Pricing

$225/0.1 mL $290/0.2 mL $465/0.5 mL $580/1 mL $1,560/5 mL $3,035/2x5 mL $4,440/3x5 mL $5,760/4x5 mL $7,055/5x5 mL

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IHC (Immunohistochemisry) (DAB staining on IHC-P; Samples: Mouse Stomach Tissue))

WB (Western Blot) (Western Blot: Sample: Recombinant protein.)

WB (Western Blot) (Western Blot: Sample: Human PC3 cell lysate; Primary Ab: 1ug/ml Rabbit Anti-Mouse IPF Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody)

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Insulin Promoter Factor 1, Polyclonal Antibody (Cat# AAA145091)

Full Name

Insulin Promoter Factor 1 (IPF) Polyclonal Antibody

Gene Names

Pdx1; Ipf1; IDX-1; IPF-1; Mody4; STF-1; pdx-1

Reactivity

Mouse, Human, Rat

Applications

Immunohistochemistry, Immunocytochemistry, Western Blot

Purity

Affinity Chromatography

Pricing

$220/0.1 mL $285/0.2 mL $450/0.5 mL $550/1 mL $1,490/5 mL $2,905/2x5 mL $4,245/3x5 mL $5,510/4x5 mL $6,725/5x5 mL

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WB (Western Blot) (Western blot analysis of lysates from 293 cells treated with UV 15', using ATRIP (Phospho-Ser224) Antibody.)

ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using ATRIP (Phospho-Ser224) Antibody)

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ATRIP, Polyclonal Antibody (Cat# AAA318287)

Full Name

ATRIP (Phospho-Ser224) Antibody

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$345/0.05 mL $450/0.1 mL $880/2x0.1 mL $1,285/3x0.1 mL $1,660/4x0.1 mL $1,775/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from Jurkat cells treated with EGF 200ng/ml 5', using FOS (Phospho-Thr232) Antibody.)

ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using FOS (Phospho-Thr232) Antibody)

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FOS, Polyclonal Antibody (Cat# AAA318318)

Full Name

FOS (Phospho-Thr232) Antibody

Gene Names

FOS; p55; AP-1; C-FOS

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$345/0.05 mL $450/0.1 mL $880/2x0.1 mL $1,285/3x0.1 mL $1,660/4x0.1 mL $1,775/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from HepG2 cells treated with PMA 125ng/ml 20', using HER2 (Phospho-Tyr1112) Antibody.)

ELISA (Enzyme-Linked Immunosorbent Assay (Phospho-ELISA) for Immunogen Phosphopeptide (Phospho-left) and Non-Phosphopeptide (Phospho-right), using HER2 (Phospho-Tyr1112) Antibody)

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HER2, Polyclonal Antibody (Cat# AAA318323)

Full Name

HER2 (Phospho-Tyr1112) Antibody

Gene Names

ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using phospho peptide. The antibody against non-phospho peptide was removed by chromatography using corresponding non-phospho peptide.

Pricing

$345/0.05 mL $450/0.1 mL $880/2x0.1 mL $1,285/3x0.1 mL $1,660/4x0.1 mL $1,775/5x0.1 mL

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WB (Western Blot) (Western blot analysis of lysates from COS7 cells, treated with sorbitol 0.4M 24h, using NF-kappaB p65 Antibody. The lane on the right is blocked with the synthesized peptide.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue, using NF-kappaB p65 Antibody. The picture on the right is blocked with the synthesized peptide.)

IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using NF-kappaB p65 Antibody. The picture on the right is blocked with the synthesized peptide.)

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NF-kappaB p65, Polyclonal Antibody (Cat# AAA318015)

Full Name

NF-kappaB p65 Antibody

Gene Names

RELA; p65; NFKB3

Reactivity

Human, Mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified from rabbit antiserum by affinity-chromatography using immunogen.

Pricing

$310/0.05 mL $390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,520/5x0.1 mL

What are Polyclonal Antibodies?

Polyclonal antibodies are antibodies that come from multiple B cell clones of a host animal. The typical hosts used for the majority of polyclonal antibody production are rabbits, goats, sheep, and donkeys. These polyclonal antibodies, once having identified their target, will bind to different epitopes located at different regions or sequences on the same protein/antigen. As a result, they are ideal at locating and binding to the target, even if the target is in very low concentrations (due to many different antibodies being able to bind to the same target molecule, which allows for significant amplification of a downstream signal).

Polyclonal antibodies are typically produced by injecting an antigen into a host animal, which causes the animal’s immune system to attack the foreign antigen by mass generating antibodies against it. After a period of time, serum is collected from the animal and purified using physicochemical fractionation, class-specific affinity purification, and/or antigen-affinity purification.

Key Uses of Polyclonal Antibodies

• Western Blotting: This method is used to find specific proteins in biological samples after separating them by size.


• Immunohistochemistry: IHC helps visualize the location of proteins in tissue sections using various staining techniques.


• ELISA: (Enzyme-Linked Immunosorbent Assay) is typically used to identify specific protein quantities in a sample. ELISAs can be either “Quantitative” or “Qualitative”.


• Flow Cytometry: technique that identifies and measures the specific protein on the surface or inside the cells in a fluid suspension.


• Immunoprecipitation: IP isolates and studies a specific protein from a complex mixture using antibodies.

Why Buy Polyclonal Antibodies from AAA Biotech?

1. Ideal for Various Applications

Our antibodies are generally going to be validated for use in multiple types of assays, including ELISA, Western Blotting, Immunohistochemistry, Immunoprecipitation, amongst others. They are ideal for a wide range of research applications.

2. Rigorous Quality Control

All of the antibodies in our catalog undergo strict quality testing to ensure specificity, sensitivity, and consistent performance. We are confident in the ability of our antibodies to provide you with accurate results.

3. Wide Assortment of Antibodies

Antibodies in are catalog can be found for both common and exotic species, and these antibodies are also available in both conjugated and recombinant forms to suit many diverse experimental needs.

4. Highly Purified

Our antibodies are available in purified forms with over 85% purity, as confirmed by SDS-PAGE. They are also available with tags such as His, Flag, GST, or MBP. We cater to customers worldwide.

FAQ

1. How are polyclonal antibodies produced?

Traditionally, polyclonal antibodies are produced by injecting an antigen into a host animal (such as a rabbit or goat), which then triggers an immune response from the host animal. The animal’s B cells produce antibodies that will recognize different parts of the injected antigen. These antibodies are then collected from the animal’s blood and purified for use.

2. How do polyclonal antibodies differ from monoclonal antibodies?

Polyclonal antibodies are a mix of antibodies that bind to different locations (epitopes) of the same antigen, while monoclonal antibodies are identical and bind to just one specific epitope. This makes polyclonal antibodies more versatile and better at detecting proteins that may be present in low quantities or in altered/modified forms.

3. How should I store polyclonal antibodies?

Polyclonal antibodies should be stored at 4°C for short-term use (up to a few weeks) and at -20°C or -80°C for long-term storage. Avoid repeated freeze-thaw cycles by dividing them into small aliquots. Always check the datasheet for specific storage instructions.