Phospho Antibodies

Phospho-specific antibodies’ typical purpose is to enable researchers to detect changes in proteins. They will exclusively bind to the amino acid sequence on a protein that has been phosphorylated (which is both a physical & chemical change) and do not bind to the same amino acid sequence on said protein if it lacks said phosphorylation. This aids in being able to clearly see and understand the data produced from this particular protein modification.

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IHC (Immunohiostchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

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Rsk-1, Polyclonal Antibody (Cat# AAA293954)

Full Name

Rsk-1 (phospho Ser380) Polyclonal Antibody

Gene Names

RPS6KA1; RSK; HU-1; RSK1; MAPKAPK1A

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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IHC (Immunohiostchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)

Compare (5 max)

hnRNP K, Polyclonal Antibody (Cat# AAA293965)

Full Name

hnRNP K (phospho Ser284) Polyclonal Antibody

Gene Names

HNRNPK; CSBP; TUNP; HNRPK

Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunohistochemistry, Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

Compare (5 max)

KIF20A, Polyclonal Antibody (Cat# AAA293968)

Full Name

KIF20A (phospho Ser528) Polyclonal Antibody

Gene Names

KIF20A; MKLP2; RAB6KIFL

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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IHC (Immunohiostchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

Compare (5 max)

Cdk5, Polyclonal Antibody (Cat# AAA293969)

Full Name

Cdk5 (phospho Tyr15) Polyclonal Antibody

Gene Names

CDK5; PSSALRE

Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunohistochemistry, Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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IHC (Immunohiostchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)

Compare (5 max)

Pdcd-4, Polyclonal Antibody (Cat# AAA293970)

Full Name

Pdcd-4 (phospho Ser67) Polyclonal Antibody

Gene Names

PDCD4; H731

Reactivity

Human, Mouse, Rat, Monkey

Applications

Immunohistochemistry, Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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IHC (Immunohiostchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)

Compare (5 max)

Lfc, Polyclonal Antibody (Cat# AAA293974)

Full Name

Lfc (phospho Ser885) Polyclonal Antibody

Gene Names

ARHGEF2; GEF; P40; GEFH1; LFP40; GEF-H1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/5000. Not yet tested in other applications.)

Compare (5 max)

TRF1, Polyclonal Antibody (Cat# AAA293975)

Full Name

TRF1 (phospho Ser219) Polyclonal Antibody

Gene Names

TERF1; TRF; PIN2; TRF1; TRBF1; t-TRF1; hTRF1-AS

Reactivity

Human, Mouse

Applications

Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

Compare (5 max)

GR, Polyclonal Antibody (Cat# AAA293978)

Full Name

GR (phospho Ser203) Polyclonal Antibody

Gene Names

NR3C1; GR; GCR; GRL; GCCR

Reactivity

Human, Mouse

Applications

Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

Compare (5 max)

Raf-1, Polyclonal Antibody (Cat# AAA293980)

Full Name

Raf-1 (phospho Ser301) Polyclonal Antibody

Gene Names

RAF1; NS5; CRAF; Raf-1; c-Raf

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/10000. Not yet tested in other applications.)

Compare (5 max)

Tie-2, Polyclonal Antibody (Cat# AAA293981)

Full Name

Tie-2 (phospho Tyr992) Polyclonal Antibody

Gene Names

TEK; TIE2; VMCM; TIE-2; VMCM1; CD202B

Reactivity

Human, Mouse

Applications

Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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Application Data (Dilution: IHC 1:100-200)

Application Data (Dilution: IHC 1:100-200)

Application Data (Dilution: IHC 1:100-200)

IHC (Immunohistochemistry) (Dilution: IHC: 1:100-200)

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Phospho-ERK 1/2, Monoclonal Antibody (Cat# AAA293783)

Full Name

Phospho-ERK 1/2 (Y222/205)Mouse Monoclonal Antibody (4G3)

Gene Names

MAPK3; ERK1; ERT2; ERK-1; PRKM3; P44ERK1; P44MAPK; HS44KDAP; HUMKER1A; p44-ERK1; p44-MAPK

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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Application Data (Dilution: IHC 1:100-200)

Application Data (Dilution: IHC 1:100-200)

Application Data (Dilution: IHC 1:100-200)

IHC (Immunohistochemistry) (Dilution: IHC: 1:100-200)

Compare (5 max)

CaMKIIbeta/gamma/delta, Monoclonal Antibody (Cat# AAA293717)

Full Name

CaMKIIbeta/gamma/delta (Phospho Thr287) Monoclonal Antibody (1B9)

Gene Names

CAMK2B; CAM2; CAMK2; CAMKB

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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WB (Western Blot) (Dilution: WB: 1:500-1000)

WB (Western Blot) (Dilution: WB: 1:500-1000)

Compare (5 max)

Histone H3, Polyclonal Antibody (Cat# AAA293570)

Full Name

Histone H3 (Phospho Thr11) Polyclonal Antibody

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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WB (Western Blot) (Dilution: WB: 1:1000-2000)

WB (Western Blot) (Dilution: WB: 1:1000-2000)

Compare (5 max)

Histone H3, Polyclonal Antibody (Cat# AAA293573)

Full Name

Histone H3 (Phospho Tyr41) Polyclonal Antibody

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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WB (Western Blot) (Dilution: WB: 1:1000)

WB (Western Blot) (Dilution: WB: 1:1000)

Compare (5 max)

Histone H3, Polyclonal Antibody (Cat# AAA293575)

Full Name

Histone H3 (Phospho Thr118) Polyclonal Antibody

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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WB (Western Blot) (Dilution: WB: 1:500-1000)

WB (Western Blot) (Dilution: WB: 1:500-1000)

Compare (5 max)

Histone H2B, Polyclonal Antibody (Cat# AAA293582)

Full Name

Histone H2B (Phospho Ser32) Polyclonal Antibody

Gene Names

HIST1H2BA; STBP; H2BFU; TSH2B; bA317E16.3

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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WB (Western Blot) (Dilution: WB: 1:1000-2000)

WB (Western Blot) (Dilution: WB: 1:1000-2000)

Compare (5 max)

Histone H2A, Polyclonal Antibody (Cat# AAA293583)

Full Name

Histone H2A (Phospho Ser129) Polyclonal Antibody

Gene Names

HIST1H2AI; H2A/c; H2AFC

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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WB (Western Blot) (Dilution: WB: 1:1000-2000)

WB (Western Blot) (Dilution: WB: 1:1000-2000)

Compare (5 max)

Histone H2B, Polyclonal Antibody (Cat# AAA293604)

Full Name

Histone H2B (Phospho Ser14) Polyclonal Antibody

Gene Names

HIST1H2BA; STBP; H2BFU; TSH2B; bA317E16.3

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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WB (Western Blot) (Dilution: WB: 1:1000-2000)

WB (Western Blot) (Dilution: WB: 1:1000-2000)

Compare (5 max)

PEPC, Polyclonal Antibody (Cat# AAA293630)

Full Name

PEPC (Phospho Ser15) Polyclonal Antibody

Gene Names

PGC; PEPC; PGII

Reactivity

Plants

Applications

Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using G3BP-1(Phospho-Ser232) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using G3BP-1(Phospho-Ser232) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from 293 cells untreated or treated with starvation using G3BP-1(Phospho-Ser232) Antibody.)

Compare (5 max)

G3BP-1, Polyclonal Antibody (Cat# AAA301654)

Full Name

G3BP-1 (Phospho-Ser232) Antibody

Gene Names

G3BP1; G3BP; HDH-VIII

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed HeLa cells using CARM1 (Phospho-Ser228) antibody (, Red).)

WB (Western Blot) (Western blot analysis of extracts from A431 cells untreated or treated with EGF (200ng/ml, 5min), using CARM1 (Ab-228) antibody (#21331, Line 1 and 2) and CARM1 (Phospho-Ser228) antibody (, Line 3 and 4).)

Compare (5 max)

CARM1, Polyclonal Antibody (Cat# AAA301724)

Full Name

CARM1(Phospho-Ser228) antibody

Gene Names

CARM1; PRMT4

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using BAD (phospho- Ser155) antibody ().)

WB (Western Blot) (Western blot analysis of extracts from 293 cells using BAD (phospho-Ser155) antibody ().)

Compare (5 max)

BAD, Polyclonal Antibody (Cat# AAA301586)

Full Name

BAD(Phospho-Ser155) Antibody

Gene Names

Bad; Bbc2; AI325008

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using PKD/PKCm(Phospho-Ser910) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from EGF-treated 293 and PMA-treated HUVEC cells using PKD/PKCm(Phospho-Ser910) Antibody.)

Compare (5 max)

PKD/PKCu, Polyclonal Antibody (Cat# AAA301373)

Full Name

PKD/PKCu (Phospho-Ser910) Antibody

Gene Names

PRKD1; PKD; PKCM; PRKCM; PKC-MU

Reactivity

Human

Applications

Immunofluorescence, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts using NF-kappaB p65 (phospho-Ser468) antibody ().)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed HeLa cells using NF-kappaB p65 (phospho-Ser468) antibody (, Red).)

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NFkappaB-p65, Polyclonal Antibody (Cat# AAA301984)

Full Name

NFkappaB-p65(Phospho-Ser468) Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using WAVE1 (Phospho-Tyr125) antibody. The picture on the right is treated with the synthesized peptide.)

WB (Western Blot) (Western blot analysis of extracts from NIH/3T3 cells, treated with Insulin (0.01U/ml, 15mins), using WAVE1 (Phospho-Tyr125) antibody. The lane on the right is treated with the synthesized peptide.)

Compare (5 max)

WAVE1, Polyclonal Antibody (Cat# AAA301843)

Full Name

WAVE1 (Phospho-Tyr125) Antibody

Gene Names

WASF1; WAVE; SCAR1; WAVE1

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

Standard Curve (Sample)

Compare (5 max)

phospho Interferon Regulatory Factor 3, ELISA Kit (Cat# AAA247212)

Full Name

Human phospho Interferon Regulatory Factor 3 ELISA Kit

Reactivity

Human

Pricing

$595/48-Strip-Wells $845/96-Strip-Wells $1,640/2x96-Strip-Wells $3,715/5x96-Strip-Wells $7,005/10x96-Strip-Wells

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF2(Phospho-Ser62 or 44) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from MDA231 cells untreated or treated with UV using ATF2(Phospho-Ser62 or 44) Antibody.)

Compare (5 max)

ATF2, Polyclonal Antibody (Cat# AAA243048)

Full Name

Phospho-ATF2 (Ser62 or 44) Antibody

Gene Names

ATF2; HB16; CREB2; TREB7; CRE-BP1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human tonsil carcinoma tissue using VASP(Phospho-Ser239) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from serum-treated 293 and PMA-treated C6 cells using VASP(Phospho-Ser239) Antibody.)

Compare (5 max)

VASP, Polyclonal Antibody (Cat# AAA243098)

Full Name

Phospho-VASP (Ser239) Antibody

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ASK1(Phospho-Ser966) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from various cells using ASK1(Phospho-Ser966) Antibody.)

Compare (5 max)

MAP3K5, Polyclonal Antibody (Cat# AAA243106)

Full Name

Phospho-MAP3K5 (Ser966) Antibody

Gene Names

MAP3K5; ASK1; MEKK5; MAPKKK5

Reactivity

Human, Mouse, Monkey

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4E-BP1(Phospho-Thr45) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from Hela cells using 4E-BP1(Phospho-Thr45) Antibody and the same antibody preincubated with blocking peptide.)

Compare (5 max)

EIF4EBP1, Polyclonal Antibody (Cat# AAA243121)

Full Name

Phospho-EIF4EBP1 (Thr45) Antibody

Gene Names

EIF4EBP1; BP-1; 4EBP1; 4E-BP1; PHAS-I

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IRS-1(Phospho-Ser636) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from 3T3 cells untreated or treated with Insulin using IRS-1(Phospho-Ser636) Antibody.)

Compare (5 max)

IRS1, Polyclonal Antibody (Cat# AAA243125)

Full Name

Phospho-IRS1 (Ser636) Antibody

Gene Names

IRS1; HIRS-1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from HT29 cells, treated with CA or calf intestinal phosphatase (CIP), using IkappaB-beta (Phospho-Ser23) Antibody.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkB-b(Phospho-Ser23) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

Compare (5 max)

NFKBIB, Polyclonal Antibody (Cat# AAA243144)

Full Name

Phospho-NFKBIB (Ser23) Antibody

Gene Names

NFKBIB; IKBB; TRIP9

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using SHP-1(Phospho-Tyr536) Antibody.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SHP-1(Phospho-Tyr536) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

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PTPN6, Polyclonal Antibody (Cat# AAA243148)

Full Name

Phospho-PTPN6 (Tyr536) Antibody

Gene Names

PTPN6; HCP; HCPH; SHP1; SHP-1; HPTP1C; PTP-1C; SHP-1L; SH-PTP1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IP (Immunoprecipitation) (Detection of human Phospho RNA Polymerase II (S2) by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: RNA Polymerase II was immunoprecipitated using rabbit anti-RNA Polymerase II antibody Following immunoprecipitation the product of the IP reaction was mock treated (-) or treated with phosphatases (+). Affinity purified rabbit anti-Phospho RNA Polymerase II (S2) antibody AAA212674 was used for WB at 1 ug/ml (upper panel). To examine total RNA Polymerase II, the blot was stripped and then blotted with rabbit anti-RNA Polymerase II antibody (lower panel). Detection: Chemiluminescence with exposure times of 30 seconds (upper panel) and 15 seconds (lower panel).)

IP (Immunoprecipitation) (Detection of human Phospho RNA Polymerase II (S2) by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: Affinity purified rabbit anti-Phospho RNA Polymerase II (S2) antibody AAA212674 (lot AAA212674-1) used for IP at 6 ug per reaction. Phospho RNA Polymerase II was also immunoprecipitated by rabbit anti-Phospho RNA Polymerase II antibodies)

IHC (Immunohiostchemistry) (Detection of human Phospho RNA Polymerase II (S2) by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Phospho RNA Polymerase II (S2) (Cat. No. AAA212674 Lot1) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)

IHC (Immunohistochemistry) (Detection of mouse Phospho RNA Polymerase II (S2) by immunohistochemistry. Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-Phospho RNA Polymerase II (S2) (Cat. No. AAA212674 Lot1) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)

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RNA Polymerase II, Polyclonal Antibody (Cat# AAA212674)

Full Name

Rabbit anti-RNA Polymerase II, Phospho (S2) Antibody, Affinity Purified

Gene Names

POLR2A; RPB1; RPO2; POLR2; POLRA; RPBh1; RPOL2; RpIILS; hsRPB1; hRPB220

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Antigen Affinity Purified

Pricing

$255/0.01 mg $635/0.1 mg $940/5x0.01 mg $1,230/2x0.1 mg $1,800/3x0.1 mg $2,335/4x0.1 mg $2,630/5x0.1 mg

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WB (Western Blot) (Detection of human Phospho SMC1 (S957) by western blot. Samples: Whole cell lysate (50 ug) from 293T cells treated with 100 uM etoposide for 2 hours (+) or mock treated (-) cells. Antibodies: Affinity purified rabbit anti-Phospho SMC1 (S957) antibody AAA212520 (lot AAA212520-1) used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower panel shows western blot for total SMC1 using affinity purified rabbit anti-SMC1 antibody at 0.1 ug/ml with an exposure time of 30 seconds.)

IP (Immunoprecipitation) (Detection of human Phospho SMC1 (S957) by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells treated with 100 uM etoposide for 2 hours (+) or mock treated (-) cells. Antibodies: Phospho SMC1 (S957) was immunoprecipitated using affinity purified rabbit anti-Phospho SMC1 (S957) antibody AAA212520 (lot AAA212520-1) at 6 ug/mg lysate. Total SMC1 was immunoprecipitated using rabbit anti-SMC1 antibody at 6 ug/mg lysate. Phospho SMC1 (S957) was blotted using AAA212520 at 0.1 ug/ml (upper panel). Total SMC1 was blotted using at 0.1 ug/ml (lower panel). Detection: Chemiluminescence with exposure times of 30 seconds.)

IHC (Immunohiostchemistry) (Detection of mouse Phospho SMC1 (S957) by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-Phospho SMC1 (S957) (Cat. No. AAA212520 Lot1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)

IHC (Immunohistochemistry) (Detection of human Phospho SMC1 (S957) by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Phospho SMC1 (S957) (Cat. No. AAA212520 Lot1) used at a dilution of 1:5,000 (0.2ug/ml). Detection: DAB)

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SMC1, Polyclonal Antibody (Cat# AAA212520)

Full Name

Rabbit anti-Phospho SMC1 (S957) Antibody, Affinity Purified

Gene Names

SMC1A; SMC1; SMCB; CDLS2; SB1.8; SMC1L1; DXS423E; SMC1alpha

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunoprecipitation, Western Blot

Purity

Antigen Affinity Purified

Pricing

$255/0.01 mg $635/0.1 mg $940/5x0.01 mg $1,230/2x0.1 mg $1,800/3x0.1 mg $2,335/4x0.1 mg $2,630/5x0.1 mg

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WB (Western Blot) (All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.)

WB (Western Blot) (All lanes use the Antibody at 1:1K dilution for 1 hour at room temperature.)

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MST1/MST2, Monoclonal Antibody (Cat# AAA128143)

Full Name

Anti-Phospho-MST1/MST2 (T183 + T180) Rabbit Monoclonal Antibody

Gene Names

STK4; KRS2; MST1; YSK3

Reactivity

Human

Applications

Western Blot

Purity

Affinity-chromatography

Pricing

$215/0.03 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,005/5x0.1 mL

WB (Western Blot) (Western blot of Arabidopsis lysate showing specific immunolabeling of the ~53 kDa S6K1 phosphorylated at Thr449 in the first lane (-). Phosphospecificity is shown in the second lane (P) where immunolabeling is blocked by preadsorption with the phosphopeptide used as antigen, but not by the corresponding non-phosphopeptide in the third lane (NP).)

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Thr449 S6 kinase 1, Polyclonal Antibody (Cat# AAA72825)

Full Name

Anti-Phospho-Thr449 S6 kinase 1 (S6K1)

Gene Names

PK1; ARABIDOPSIS THALIANA PROTEIN-SERINE KINASE 1; ARABIDOPSIS THALIANA PROTEIN-SERINE KINASE 6; ATPK1; ATPK6; ATS6K1; P70 RIBOSOMAL S6 KINASE; PK1; PK6; PROTEIN-SERINE KINASE; protein-serine kinase 1; ROTEIN-SERINE KINASE 6; S6K1

Reactivity

Arabidopsis

Applications

Western Blot

Purity

Antigen Affinity Purified from the Pooled Serum

Pricing

$500/0.1 mL $975/2x0.1 mL $1,430/3x0.1 mL $1,855/4x0.1 mL $2,060/5x0.1 mL

Application Data

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JAK2 (pY1007/Y1008) (Biotinylated), Antibody (Cat# AAA71402)

Full Name

Rabbit anti Phospho- JAK2 (pTyr 1007/1008)Biotinylated

Gene Names

JAK2; JTK10

Reactivity

Human, Rat, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

The Rabbit IgG is purified by site-modified Epitope Affinity Purification.

Pricing

$365/0.1 mg $705/2x0.1 mg $1,030/3x0.1 mg $1,335/4x0.1 mg $1,420/5x0.1 mg

Application Data

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GSK3b (Paired S9), Antibody (Cat# AAA71385)

Full Name

Rabbit anti GSK-3beta (Paired Ser9), Non-phospho

Gene Names

GSK3B; GSK3B

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The Rabbit IgG is purified by site-modified Epitope Affinity Purification.

Pricing

$345/0.1 mg $665/2x0.1 mg $965/3x0.1 mg $1,255/4x0.1 mg $1,315/5x0.1 mg

Application Data

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Dynamin (Ser778), Polyclonal Antibody (Cat# AAA72786)

Full Name

Anti-Phospho-Ser778 Dynamin

Gene Names

Dnm1; Dnm

Reactivity

Rat

Applications

Western Blot

Purity

Affinity Purified (Prepared from sheep serum by affinity purification via sequential chromatography on phospho- and dephosphopeptide affinity columns.)

Pricing

$500/0.1 mL $975/2x0.1 mL $1,430/3x0.1 mL $1,855/4x0.1 mL $2,060/5x0.1 mL

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ICC (Immunocytochemistry) (Immunocytochemical labeling of CRMP2 phosphorylation in aldehyde-fixed and NP-40-permeabilized NGF-differentiated PC12 cells. The cells were labeled with rabbit polyclonal anti-CRMP2 (Ser-522) (CP2191) antibody in the absence (Left) or presence (Right) of blocking peptide (CX2195). The antibody was detected using appropriate secondary antibody conjugated to DyLight 594.)

WB (Western Blot) (Western blot image of mouse brain untreated (lanes 1 & 3) or treated with lambda phosphatase (lanes 2 & 4). The blot was probed with anti-CRMP2 (C-terminal Region) (lanes 1 & 2) or anti-CRMP2 (Ser-522) (lanes 3 & 4).)

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CRMP2, Polyclonal Antibody (Cat# AAA71604)

Full Name

CRMP2 (Ser-522), phospho-specific Antibody

Gene Names

DPYSL2; DRP2; N2A3; CRMP2; DRP-2; ULIP2; CRMP-2; DHPRP2; ULIP-2

Reactivity

Human, Rat, Mouse

Applications

Immunocytochemistry, Western Blot

Pricing

$505/0.1 mL $985/2x0.1 mL $1,440/3x0.1 mL $1,875/4x0.1 mL $2,100/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Arrestin 1 (Phospho-Ser412) antibody. Western blot analysis of extracts from COS7 cells, treated with Etoposide (25uM, 60mins), using Arrestin 1 (Phospho-Ser412) antibody.)

Application Data (P-peptide-+)

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Arrestin 1, Antibody (Cat# AAA109030)

Full Name

Arrestin 1 (Phospho-Ser412) Antibody

Gene Names

ARRB1; ARB1; ARR1

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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IF (Immunofluorescence) (Immunofluorescent analysis of GSK3 alpha (pS21) staining in PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of GSK3 alpha (pS21) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of GSK3 alpha (pS21) expression in HEK293T UV-treated (A), mouse liver (B), rat liver (C), PC12 H2O2-treated (D) whole cell lysates.)

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GSK3 alpha (pS21), Polyclonal Antibody (Cat# AAA104249)

Full Name

Anti-GSK3 alpha (pS21) Phospho Antibody

Reactivity

Human, Mouse, Rat

Applications

Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$240/0.03 mL $275/0.05 mL $345/0.1 mL $475/0.2 mL $925/2x0.2 mL $1,355/3x0.2 mL $1,755/4x0.2 mL $1,920/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescent analysis of G3BP1 (pS232) staining in A549 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of G3BP1 (pS232) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of G3BP1 (pS232) expression in A549 (A) whole cell lysates.)

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G3BP1 (pS232), Polyclonal Antibody (Cat# AAA104274)

Full Name

Anti-G3BP1 (pS232) Phospho Antibody

Gene Names

G3BP1; G3BP; HDH-VIII

Reactivity

Human

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$240/0.03 mL $275/0.05 mL $345/0.1 mL $475/0.2 mL $925/2x0.2 mL $1,355/3x0.2 mL $1,755/4x0.2 mL $1,920/5x0.2 mL

WB (Western Blot) (Western blot analysis of TAU (pS579) expression in A549 (A), SP2/0 (B) whole cell lysates.)

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TAU (pS262), Polyclonal Antibody (Cat# AAA104279)

Full Name

Anti-TAU (pS579) Phospho Antibody

Gene Names

MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103

Reactivity

Human, Mouse, Bovine, Chicken, Rabbit, Zebrafish

Applications

Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$240/0.03 mL $275/0.05 mL $345/0.1 mL $475/0.2 mL $925/2x0.2 mL $1,355/3x0.2 mL $1,755/4x0.2 mL $1,920/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of SMAD1 (pS187) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of SMAD1 (pS187) expression in HEK293T BMP4-treated (A), HeLa PMA-treated (B), A673 (C), PC12 (D) whole cell lysates.)

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SMAD1 (pS187), Polyclonal Antibody (Cat# AAA104435)

Full Name

Anti-SMAD1 (pS187) Phospho Antibody

Gene Names

SMAD1; BSP1; JV41; BSP-1; JV4-1; MADH1; MADR1

Reactivity

Human, Mouse, Rat, Chicken, Sheep

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$240/0.03 mL $275/0.05 mL $345/0.1 mL $475/0.2 mL $925/2x0.2 mL $1,355/3x0.2 mL $1,755/4x0.2 mL $1,920/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescent analysis of AS160 (pT642) staining in Raw264.7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of AS160 (pT642) staining in human brain formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of AS160 (pT642) expression in LO2 insulin-treated (A), Raw264.7 insulin-treated (B), PC12 insulin-treated (C), NIH3T3 UV-treated (D) whole cell lysates.)

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AS160 (pT642), Polyclonal Antibody (Cat# AAA104296)

Full Name

Anti-AS160 (pT642) Phospho Antibody

Gene Names

TBC1D4; AS160

Reactivity

Human, Mouse, Rat

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$240/0.03 mL $275/0.05 mL $345/0.1 mL $475/0.2 mL $925/2x0.2 mL $1,355/3x0.2 mL $1,755/4x0.2 mL $1,920/5x0.2 mL

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed HeLa cells using Keratin 8 (Phospho-Ser74) Antibody)

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Keratin 8, Antibody (Cat# AAA110972)

Full Name

Keratin 8 (Phospho-Ser74) Antibody

Gene Names

KRT8; K8; KO; CK8; CK-8; CYK8; K2C8; CARD2

Reactivity

Human

Applications

Immunofluorescence

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$305/0.05 mg $390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using Opioid Receptor-delta (Phospho-Ser363) antibody. Western blot analysis of extracts from 293 cells, treated with TSA (400nM, 24hours), using Opioid Receptor-delta (Phospho-Ser363) antibody.)

Application Data (P-peptide-+)

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Opioid Receptor-delta, Antibody (Cat# AAA112152)

Full Name

Opioid Receptor-delta (Phospho-Ser363) Antibody

Gene Names

OPRD1; OPRD

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF405M) $580/0.1 mL (AF750) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using Retinoic Acid Receptor alpha (Phospho-Ser77) antibody. Western blot analysis of extracts from Jurkat cells treated with PMA (125ng/ml, 30mins), using Retinoic Acid Receptor alpha (Phospho-Ser77) antibody.)

Application Data (P-peptide-+)

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Retinoic Acid Receptor alpha, Antibody (Cat# AAA112033)

Full Name

Retinoic Acid Receptor alpha (Phospho-Ser77)Antibody

Gene Names

RARA; RAR; NR1B1

Reactivity

Human (Identities= 100%, Positives= 100%)
Mouse (Identities 100%, Positives= 100%)
Rat (Identities= 100%, Positives 100%)

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody againstnon-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

What Are Phospho Antibodies?

Protein phosphorylation is a process where a phosphate group is added to certain amino acid residues of a protein – usually serine (S), threonine (T), or tyrosine (Y) - by enzymes called kinases. This process is integral in controlling cellular signaling, cellular growth, and other biological functions.

Our catalog includes a wide range of phospho-specific antibodies that can accurately detect this important marker. They perform strongly in widely-used laboratory applications such as Western blot, flow cytometry, immunohistochemistry, and immunofluorescence microscopy. We value your trust in us and are committed to providing top-quality products and services. All of our antibodies are guaranteed to work for the applications and species indicated on our website & associated product pages.

What Are The Key Applications of Phospho Antibodies?

1. Western Blotting

One of the first steps a researcher can take in utilizing these phospho-specific antibodies, is to check if the antibody works using a technique referred to as “Western blot”. For those unfamiliar, Western Blot aids in showing whether the protein that the antibody recognizes is appearing at the correct/expected size. These phospho-specific antibodies should also be able to detect changes in the target protein’s phosphorylation (on/off state) when cells are stimulated in certain ways.

2. Staining of Fixed Cells (Immunocytochemistry)

Another routine use of these phospho-specific antibodies, is to test if the antibody is able to demonstrate similar performance when used on fixed cells (intact cells that have been preserved) as it did in the Western blot tests. It is an important aspect in many cases to confirm that the antibody works in actual intact cell samples. Ideally, the method used for cellular fixation should be the same as what is used in pathology labs (like using 10% formalin). To check if the antibody works well in tissue sections (FFPE), researchers will often test it on fixed cells that are processed similar to tissue samples.

3. Specificity Tests Using Peptides

In order to make sure that the antibody is only binding to the right target:

• Laboratory technicians will mix the antibody with phospho-peptides (short segments of the protein containing the phosphate group modification).


• If the antibody signal disappears, it is confirmation that it is binding to the correct phosphorylated location.


• A more robust test is to use both the phosphorylated and non-phosphorylated (dephosphorylated) versions of the protein. The antibody should react only with the phosphorylated one.


• Another method sometimes utilized is to treat the sample with an enzyme, such as alkaline phosphatase, that specifically removes phosphate groups. If the antibody signal disappears after this, it also confirms specificity.

4. Genetic Confirmation

As a final step, scientists can genetically manipulate the nucleotide sequence and alter the target protein by removing the exact site where phosphorylation happens. If the antibody no longer appears to detect the modified protein, it is strong evidence supporting the antibody being specific for that phosphorylated site.

Why Buy Phospho Antibodies Through Us?

• The production laboratory adheres to strict and consistent protocols prior to releasing any of these phospho-specific antibodies:


• Standard methods and proper controls in all tests to ensure high quality.


• These antibodies are tested and validated in different cell types and species.


• High quality control criterion to ensure each batch is consistent, so you will obtain reliable results every time.

FAQ

1. What Are Phospho-Specific Antibodies?

Phospho-specific antibodies are made to detect proteins only when they have a phosphate group linked to a specific amino acid residue. This empowers scientists understand if a protein is "turned on" or active, based on its phosphorylation state.

2. How to Detect Phosphorylated Proteins in a Western Blot?

To find out if a protein is phosphorylated using Western blot:

• Use a phospho-specific antibody that binds only to the phosphorylated form of the protein.


• You can also use a “regular” antibody for the same amino acid sequence of the protein that the phospho-specific antibody is binding to (but in this case, this antibody will not bind if there is a phosphate group present) in order to compare how much of it is phosphorylated versus how much is non-phosphorylated (or “total” protein, if the “normal” antibody’s epitopes are non-phospho-site-specific).

3. How to Choose the Best Antibody?

Here are some simple tips to help you pick the right antibody:

• Know your target


• Match your sample characteristics


• Confirm the intended use is appropriate


• Check “host” and “type”


• Check the “quality” of the presented data/images


• Appraise whether the available validation meets your needs