Phospho Antibodies

Phospho-specific antibodies’ typical purpose is to enable researchers to detect changes in proteins. They will exclusively bind to the amino acid sequence on a protein that has been phosphorylated (which is both a physical & chemical change) and do not bind to the same amino acid sequence on said protein if it lacks said phosphorylation. This aids in being able to clearly see and understand the data produced from this particular protein modification.

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using GATA1(Phospho-Ser310) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from HT29 cells untreated or treated with Anisomycin using GATA1(Phospho-Ser310) Antibody.)

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GATA1, Polyclonal Antibody (Cat# AAA301872)

Full Name

GATA1 (Phospho-Ser310) Antibody

Gene Names

GATA1; GF1; GF-1; NFE1; XLTT; ERYF1; NF-E1; XLANP; XLTDA; GATA-1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p90 RSK (Phospho-Thr573) antibody (left)or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from 293 cells treated with UV using p90 RSK (Phospho-Thr573) Antibody.The lane on the right is treated with the antigen-specific peptide.)

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p90 RSK, Polyclonal Antibody (Cat# AAA301889)

Full Name

p90 RSK (Phospho-Thr573) Antibody

Gene Names

RPS6KA1; RSK; HU-1; RSK1; p90Rsk; MAPKAPK1A

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from JK cells (Lane 2), using TAL-1 (Phospho-Ser122) Antibody. The lane on the left is treated with synthesized peptide.)

WB (Western Blot) (Western blot analysis of extracts from Jurkat cells, treated with PMA (125 ng/ml, 30mins), using TAL-1 (Phospho-Ser122) antibody.)

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TAL-1 (Phospho-Ser122), Polyclonal Antibody (Cat# AAA289522)

Full Name

TAL-1 (Phospho-Ser122) Antibody

Gene Names

TAL1; SCL; TCL5; tal-1; bHLHa17

Applications

Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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WB (Western Blot) (Dilution: WB: 1:1000-2000)

WB (Western Blot) (Dilution: WB: 1:1000-2000)

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Histone H1, Polyclonal Antibody (Cat# AAA293602)

Full Name

Histone H1 (Phospho Thr3) Polyclonal Antibody

Gene Names

HIST1H1A; H1A; H1.1; H1F1; HIST1

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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WB (Western Blot) (Dilution: WB: 1:1000-2000)

WB (Western Blot) (Dilution: WB: 1:1000-2000)

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Histone H1, Polyclonal Antibody (Cat# AAA293603)

Full Name

Histone H1 (Phospho Thr17) Polyclonal Antibody

Gene Names

HIST1H1A; H1A; H1.1; H1F1; HIST1

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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WB (Western Blot) (Dilution: WB: 1:1000)

WB (Western Blot) (Dilution: WB: 1:1000)

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Histone H3, Polyclonal Antibody (Cat# AAA293568)

Full Name

Histone H3 (Phospho Thr6) Polyclonal Antibody

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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WB (Western Blot) (Dilution: WB: 1:500-1000)

WB (Western Blot) (Dilution: WB: 1:500-1000)

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Histone H3, Polyclonal Antibody (Cat# AAA293569)

Full Name

Histone H3 (Phospho Ser10) Polyclonal Antibody

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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WB (Western Blot) (Dilution: WB: 1:200-500)

WB (Western Blot) (Dilution: WB: 1:200-500)

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Histone H3, Polyclonal Antibody (Cat# AAA293571)

Full Name

Histone H3 (Phospho Ser28) Polyclonal Antibody

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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WB (Western Blot) (Dilution: WB: 1:1000-2000)

WB (Western Blot) (Dilution: WB: 1:1000-2000)

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Histone H3, Polyclonal Antibody (Cat# AAA293572)

Full Name

Histone H3 (Phospho Thr32) Polyclonal Antibody

Gene Names

HIST1H3A; H3/A; H3FA

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from A431 cells,untreated or treated with EGF,100ng/ml, using phospho-ERBB2-Y1221(left) or ERBB2 antibody(right))

WB (Western Blot) (Western blot analysis of extracts from A431 cells,untreated or treated with EGF,100ng/ml?using phospho ErbB2-Y1221(left) or ErbB2 antibody(right))

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Phospho-ErbB2 (Y1221), Polyclonal Antibody (Cat# AAA286938)

Full Name

Phospho-ErbB2 (Y1221) Antibody

Gene Names

ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu

Reactivity

Human

Applications

Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue, using Mst1/2 (Phospho-Thr183) antibody.)

WB (Western Blot) (Western blot analysis of extracts from 293 cells, treated with H2O2 (100uM, 15mins), using Mst1/2 (Phospho-Thr183) antibody.)

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Mst1/2 (Phospho-Thr183), Polyclonal Antibody (Cat# AAA286969)

Full Name

Mst1/2 (Phospho-Thr183) Antibody

Gene Names

STK3; KRS1; MST2

Reactivity

Human, rat

Applications

Immunohistochemistry, Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma)

WB (Western Blot) (The anti-phospho-CDC25B-S353 Pab is used in Western blot to detect phospho-CDC25B-S353 in HEPG2 tissue lysate)

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Phospho-CDC25B (S353), Polyclonal Antibody (Cat# AAA286525)

Full Name

Phospho-CDC25B (S353) Antibody

Reactivity

Human

Applications

Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IF (Immunofluorescence) (Confocal immunofluorescent analysis of Phospho-AKT1 (Thr308) Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green).Actin filaments have been labeled with Alexa Fluor 555 phalloidin (red).DAPI was used to stain the cell nuclear (blue).)

DB (Dot Blot) (Dot blot analysis of anti-Phospho-AKT1 (Thr308) Antibody Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)

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Phospho-AKT1 (Thr308)), Polyclonal Antibody (Cat# AAA286709)

Full Name

Phospho-AKT1 (Thr308)) Antibody

Gene Names

AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Reactivity

Human

Applications

Immunofluorescence, Dot Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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ICC (Immunocytochemistry) (Immunofluorescent analysis of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cells using Phospho-Histone H2A.X (Ser139) mouse mAb (1:400).)

WB (Western Blot) (Western blot detection of Phosphorylation of H2A.X at Serine 139 in 3T3 or Hydroxyurea-treated 3T3 cell lysates using Phospho-Histone H2A.X (Ser139) mouse mAb (1:2000 diluted).Predicted band size:15KDa.Observed band size:15KDa.)

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Histone H2A.X, Monoclonal Antibody (Cat# AAA290463)

Full Name

Phospho-Histone H2A.X (Ser139) Antibody

Gene Names

H2AFX; H2AX; H2A.X; H2A/X

Reactivity

Mouse

Applications

Immunocytochemistry, Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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IF (Immunofluorescence) (Confocal immunofluorescent analysis of Phospho-ABL-Y185 Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

DB (Dot Blot) (Dot blot analysis of anti-ABL-pY185 Pab (RB07973) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

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Phospho-ABL (Y185), Polyclonal Antibody (Cat# AAA290156)

Full Name

Phospho-ABL (Y185) Antibody

Gene Names

ABL1; ABL; JTK7; p150; c-ABL; v-abl; c-ABL1; bcr/abl

Reactivity

Human (Predicted Reactivity: Mouse)

Applications

Immunofluorescence, Dot Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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DB (Dot Blot) (Dot blot analysis of Phospho-ERBB2-Y1127 Antibody Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.6ug per ml.)

IF (Immunofluorescence) (Fluorescent confocal image of MCF7 cells stained with phospho-ERBB2-Y1127 antibody. MCF7 cells were fixed with 4% PFA (20 min), permeabilized with Triton X-100 (0.2%, 30 min). Cells were then incubated with AAA290180 phospho-ERBB2- Y1127 primary antibody (1:100, 2 h at room temperature). For secondary antibody, Alexa Fluor 488 conjugated donkey anti-rabbit antibody (green) was used (1:1000, 1h). Nuclei were counterstained with Hoechst 33342 (blue) (10 ug/ml, 5 min). Note the highly specific localization of the phospho-ERBB2-Y1127 to the plasma membrane and cytoplasm.)

WB (Western Blot) (Western blot analysis of extracts from A431 cells,untreated or treated with EGF,100ng/ml ?using phospho ERBB2-Y1127(left) or ERBB2 antibody(right))

WB (Western Blot) (Western blot analysis of extracts from A431 cells, untreated or treated with EGF, 100ng/ml, using Phospho-ERBB2-Y1127 or ERBB2 antibody(right).)

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Phospho-ERBB2 (Y1127), Polyclonal Antibody (Cat# AAA290180)

Full Name

Phospho-ERBB2 (Y1127) Antibody

Gene Names

ERBB2; NEU; NGL; HER2; TKR1; CD340; HER-2; MLN 19; HER-2/neu

Reactivity

Human

Applications

Dot Blot, Immunofluorescence, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded rat hippocampal region tissue from a model with Alzheimer)

WB (Western Blot) (Western blot analysis of extracts from mouse brain tissue using Tau(Phospho-Ser396) Antibody and the same antibody preincubated with blocking peptide.)

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Tau, Polyclonal Antibody (Cat# AAA301111)

Full Name

Tau (Phospho-Ser396) Antibody

Gene Names

MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

WB (Western Blot) (Western blot analysis of VEC using p-cPLA2 (S505) antibody.)

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cPLA2, Polyclonal Antibody (Cat# AAA301124)

Full Name

cPLA2 (Phospho-Ser505) Polyclonal Antibody

Gene Names

PLA2G4A; cPLA2; PLA2G4; cPLA2-alpha

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4E-BP1(Phospho-Thr45) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from Hela cells using 4E-BP1(Phospho-Thr45) Antibody and the same antibody preincubated with blocking peptide.)

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4E-BP1, Polyclonal Antibody (Cat# AAA301172)

Full Name

4E-BP1 (Phospho-Thr45) Antibody

Gene Names

EIF4EBP1; BP-1; 4EBP1; 4E-BP1; PHAS-I

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

Standard Curve (Sample)

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ptau, ELISA Kit (Cat# AAA164996)

Full Name

Rat ptau (phospho Tau Protein) ELISA Kit

Reactivity

Rat

Pricing

$225/24 Tests (LIMIT 1) $575/48 Tests $675/96 Tests $1,315/2x96 Tests $1,920/3x96 Tests $2,485/4x96 Tests $2,850/5x96 Tests $5,090/10x96 Tests

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WB (Western Blot) (All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.)

WB (Western Blot) (All lanes use the Antibody at 1:3K dilution for 1 hour at room temperature.)

WB (Western Blot) (Western blot analysis of Phospho-Src (Y529) expression in A431 cell treated with EGF lysate.)

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Src, Monoclonal Antibody (Cat# AAA128108)

Full Name

Anti-Phospho-Src (Y529) Rabbit Monoclonal Antibody

Gene Names

SRC; ASV; SRC1; c-SRC; p60-Src

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunocytochemistry, Western Blot

Purity

Affinity-chromatography

Pricing

$215/0.03 mL $485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,005/5x0.1 mL

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IP (Immunoprecipitation) (Detection of human Phospho-MEK1 (T286) by immunoprecipitation (IP). Samples: 50 ug HeLa whole cell lysate (WCL) from mock treated (-) or nocodazole treated (+) cells. 1 mg of HeLa WCL mock treated (-) or nocodazole treated (+) and immunoprecipitated; 20% of the IP was loaded. Antibodies: Affinity purified goat anti-phospho MEK1 (T286) AAA212238 was used for IP (6ug/1mg lysate). To detect total MEK1, Rabbit anti-MEK1 (BL8445) was used for western blot (WB) at 1 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.)

IHC (Immunohiostchemistry) (Detection of mouse Phospho-MEK1 (T286) by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified goat anti-phospho MEK1 (T286) (Cat. No. AAA212238 lot 1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)

IHC (Immunohistochemistry) (Detection of human Phospho-MEK1 (T286) by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified goat anti-phospho MEK1 (T286) (Cat. No. AAA212238 lot 1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)

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MEK1, Polyclonal Antibody (Cat# AAA212238)

Full Name

Goat anti-Phospho MEK1 (T286) Antibody, Affinity Purified

Gene Names

MAP2K1; MEK1; MKK1; MAPKK1; PRKMK1

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunoprecipitation

Purity

Antigen Affinity Purified

Pricing

$255/0.01 mg $635/0.1 mg $940/5x0.01 mg $1,230/2x0.1 mg $1,800/3x0.1 mg $2,335/4x0.1 mg $2,630/5x0.1 mg

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IP (Immunoprecipitation) (Detection of Phosphorylation of human ZAP70 on Y319, Y493 or Y493/Y494 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells that had been treated with hydrogen peroxide. Antibodies: Affinity purified rabbit anti-Phospho ZAP70 antibodies (for phosphorylation at Y319), (for phosphorylation at Y493), and AAA211720 (for phosphorylation at Y493/Y494) used for IP at 3 ug/mg lysate. For blotting immunoprecipitated Phospho ZAP70, antibody (for total ZAP70) was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)

IP (Immunoprecipitation) (Detection of Phosphorylation of human ZAP70 on Y493/Y494 by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells that had been treated with hydrogen peroxide (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-ZAP70 antibody was used at 3 ug/mg lysate to immunoprecipitate ZAP70. The immunoprecipitates were treated with phosphatase (+) or mock (-) treated. For blotting immunoprecipitated Phospho ZAP70, anti-Phospho ZAP70 (Y493/Y494) antibody AAA211720 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 10 seconds.)

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ZAP70, Polyclonal Antibody (Cat# AAA211720)

Full Name

Rabbit anti-Phospho ZAP70 (Y493/Y494) Antibody, Affinity Purified

Gene Names

ZAP70; SRK; STD; TZK; STCD; ZAP-70

Reactivity

Human

Applications

Immunoprecipitation

Purity

Antigen Affinity Purified

Pricing

$255/0.01 mg $635/0.1 mg $940/5x0.01 mg $1,230/2x0.1 mg $1,800/3x0.1 mg $2,335/4x0.1 mg $2,630/5x0.1 mg

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WB (Western Blot) (Detection of human phospho-BRCA1 (S1280) by western blot. Samples: Whole cell lysate (50 ug) from HeLa cells untreated (-) or treated (+) with Hydroxyurea. Antibody: Affinity purified rabbit anti-phospho-BRCA1 (S1280) antibody (AAA210718 lot 2) used for WB at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.)

IP (Immunoprecipitation) (Detection of human phospho-BRCA1 (S1280) by western blot of immunoprecipitates. Samples: Whole cell lysate (1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells treated with Hydroxyurea. Antibodies: Affinity purified rabbit anti-phospho-BRCA1 (S1280) antibody (AAA210718 lot 2) used for IP at 6 ug per reaction. Phospho-BRCA1 (S1280) was also immunoprecipitated by a second phospho-BRCA1 antibody . For blotting immunoprecipitated phospho-BRCA1 (S1280), AAA210718 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.)

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BRCA1, Polyclonal Antibody (Cat# AAA210718)

Full Name

Rabbit anti-Phospho BRCA1 (S1280) Antibody, Affinity Purified

Gene Names

BRCA1; IRIS; PSCP; BRCAI; BRCC1; PNCA4; RNF53; BROVCA1; PPP1R53

Reactivity

Human

Applications

Immunoprecipitation, Western Blot

Purity

Antigen Affinity Purified

Pricing

$255/0.01 mg $635/0.1 mg $940/5x0.01 mg $1,230/2x0.1 mg $1,800/3x0.1 mg $2,335/4x0.1 mg $2,630/5x0.1 mg

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IF (Immunofluorescence) (Immunofluorescence analysis of HeLa cells, using IRF-3 (Phospho-Ser385) antibody.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using IRF-3 (Phospho-Ser385) antibody.)

WB (Western Blot) (Western blot analysis of extracts from HT-29 cells, treated with insulin (0.01U/ml, 15mins), using IRF-3 (Phospho-Ser385) antibody.)

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IRF-3 (Phospho-Ser385), Polyclonal Antibody (Cat# AAA286034)

Full Name

IRF-3 (Phospho-Ser385) Antibody

Reactivity

Human, mouse, rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from 3T3 cells (Lane 2), A549 cells (Lane 3) and Hela cells (Lane 4), using MAP3K7 (Phospho-Thr187) Antibody. The lane on the left is treated with synthesized peptide.)

WB (Western Blot) (Western blot analysis of extracts from 3T3 cells, treated with heat shock, using MAP3K7 (Phospho-Thr187) antibody.)

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MAP3K7 (Phospho-Thr187), Polyclonal Antibody (Cat# AAA286076)

Full Name

MAP3K7 (Phospho-Thr187) Antibody

Gene Names

MAP3K7; TAK1; MEKK7; TGF1a

Reactivity

Human, mouse, rat

Applications

Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using SF1 (Phospho-Ser82) antibody)

WB (Western Blot) (Western blot analysis of extracts from HUVEC cells, treated with anisomycin (25ug/ml, 30mins), using SF1 (Phospho-Ser82) antibody.)

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SF1 (Phospho-Ser82), Polyclonal Antibody (Cat# AAA286097)

Full Name

SF1 (Phospho-Ser82) Antibody

Gene Names

SF1; BBP; MBBP; ZFM1; ZNF162; D11S636; ZCCHC25

Reactivity

Human, mouse, rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

DB (Dot Blot) (Dot blot analysis of anti-Phospho-P21CIP1-T57 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Compare (5 max)

Phospho-P21CIP1 (T57), Polyclonal Antibody (Cat# AAA286194)

Full Name

Phospho-P21CIP1 (T57) Antibody

Gene Names

CDKN1A; P21; CIP1; SDI1; WAF1; CAP20; CDKN1; MDA-6; p21CIP1

Reactivity

Human

Applications

Immunohistochemistry, Dot Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

DB (Dot Blot) (Dot blot analysis of anti-Phospho-EP300-S89 Pab on nitrocellulose membrane. 50ng of Phospho-peptide (BP3197a) or Non Phospho-peptide per dot were adsorbed. Antobodies working concentration was 0.5ug per ml.)

Compare (5 max)

Phospho-EP300 (S89), Polyclonal Antibody (Cat# AAA285737)

Full Name

Phospho-EP300 (S89) Antibody

Gene Names

EP300; p300; KAT3B; RSTS2

Reactivity

Human (Predicted Reactivity: Mouse)

Applications

Dot Blot, Immunohistochemistry

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

DB (Dot Blot) (Dot blot analysis of Phospho-PI3KC3-S164 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Compare (5 max)

Phospho-PI3KC3 (S164), Polyclonal Antibody (Cat# AAA285751)

Full Name

Phospho-PI3KC3 (S164) Antibody

Gene Names

PIK3C3; VPS34; Vps34; hVps34

Reactivity

Human (Predicted Reactivity: Mouse, Pig, Rat)

Applications

Dot Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

DB (Dot Blot) (Dot blot analysis of anti-Phospho-CLDN2-pY224 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Compare (5 max)

Phospho-CLDN2 (Y224), Polyclonal Antibody (Cat# AAA286155)

Full Name

Phospho-CLDN2 (Y224) Antibody

Reactivity

Human

Applications

Dot Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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WB (Western Blot) (Western blot analysis of EGFR (arrow) in Hela cell lysates, either induced (Lane 1) or noninduced with EGF (Lane 2).)

DB (Dot Blot) (Dot blot analysis of anti-EGFR-pY1092 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Compare (5 max)

Phospho-EGFR (Y1092), Polyclonal Antibody (Cat# AAA286161)

Full Name

Phospho-EGFR (Y1092) Antibody

Gene Names

EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2

Reactivity

Human

Applications

Western Blot, Dot Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

< >

<< Previous Next >>

DB (Dot Blot) (Dot blot analysis of anti-RPS6KA1-pT359 Pab (RB13385) on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

WB (Western Blot) (Western blot analysis of extracts from Hela cells,untreated or treated with TPA,200nM?using phospho RPS6KA1-T359 (left) or RPS6KA1 antibody(right))

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Phospho-RPS6KA1 (T359), Polyclonal Antibody (Cat# AAA285533)

Full Name

Phospho-RPS6KA1 (T359) Antibody

Gene Names

RPS6KA1; RSK; HU-1; RSK1; p90Rsk; MAPKAPK1A

Reactivity

Human (Predicted Reactivity: Chicken, Xenopus)

Applications

Western Blot, Dot Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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<< Previous Next >>

DB (Dot Blot) (Dot blot analysis of anti-EGFR B1 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

WB (Western Blot) (Western blot analysis of lysate from A431 cells(from left to right),untreated or treated with EGF at 100ng/ml,using Phospho-EGFR-pY1016 Antibody or EGFR-pS695 Antibody.Lysate at 15ug per lane.AAA287360 was diluted at 1:8000 dilution at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:5000 dilution was used as the secondary antibody.)

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Phospho-EGFR (Y1016), Polyclonal Antibody (Cat# AAA287360)

Full Name

Phospho-EGFR (Y1016) Antibody

Gene Names

EGFR; ERBB; HER1; mENA; ERBB1; PIG61; NISBD2

Reactivity

Human (Predicted Reactivity: Mouse)

Applications

Western Blot, Dot Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of extracts from NIH-3T3 cells,untreated or treated with PDGF,using Phospho-Akt(Ser473)(left) or Akt antibody(right).)

DB (Dot Blot) (Dot blot analysis of anti-AKT1-pS473 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Compare (5 max)

Phospho-AKT1 (S473), Polyclonal Antibody (Cat# AAA287438)

Full Name

Phospho-AKT1 (S473) Antibody

Gene Names

AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Reactivity

Human

Applications

Dot Blot, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

DB (Dot Blot) (Dot blot analysis of Phospho-NR4A1-S351 polyclonal antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentration was 0.5ug per ml. P-Pab: phospho-antibody; P-Peptide: phospho-peptide; NP-Peptide: non-phospho-peptide.)

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Phospho-NR4A1 (S351), Polyclonal Antibody (Cat# AAA287157)

Full Name

Phospho-NR4A1 (S351) Antibody

Gene Names

NR4A1; HMR; N10; TR3; NP10; GFRP1; NAK-1; NGFIB; NUR77

Reactivity

Human (Predicted Reactivity: Mouse, Rat)

Applications

Dot Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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<< Previous Next >>

IHC (Immunohistochemisry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot) (Western blot analysis of anti-Phospho-cJun-pS63 Pab in mouse brain tissue lysate (35ug/lane). Mouse Phospho-cJun-pS63(arrow) was detected using the purified Pab.)

DB (Dot Blot) (Dot blot analysis of anti-Phospho-cJun-S63 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Compare (5 max)

Phospho-cJun (S63), Polyclonal Antibody (Cat# AAA287158)

Full Name

Phospho-cJun (S63) Antibody

Gene Names

JUN; AP1; AP-1; c-Jun

Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Dot Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

DB (Dot Blot) (Dot blot analysis of anti-Phospho-RB-S608 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

Compare (5 max)

Phospho-RB (S608), Polyclonal Antibody (Cat# AAA287060)

Full Name

Phospho-RB (S608) Antibody

Gene Names

RB1; RB; pRb; OSRC; pp110; p105-Rb; PPP1R130

Reactivity

Human

Applications

Immunohistochemistry, Dot Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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<< Previous Next >>

IF (Immunofluorescence) (Confocal immunofluorescent analysis of Phospho-ABL1-Y251 Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

IHC (Immunohistochemisry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot) (Western blot analysis of anti-ABL Pab in Hela cell line lysate (35ug/lane). ABL(arrow) was detected using the purified Pab.)

DB (Dot Blot) (Dot blot analysis of anti-Phospho-ABL1-Y251 Antibody on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antobodies working concentration was 0.5ug per ml.)

Compare (5 max)

Phospho-ABL1 (Y251), Polyclonal Antibody (Cat# AAA287492)

Full Name

Phospho-ABL1 (Y251) Antibody

Gene Names

ABL1; ABL; JTK7; p150; c-ABL; v-abl; c-ABL1; bcr/abl

Reactivity

Human

Applications

Immunohistochemistry, Western Blot, Dot Blot, Immunofluorescence

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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<< Previous Next >>

IHC (Immunohistochemisry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using MEF2C (Phospho-Ser396) antibody.)

WB (Western Blot) (Western blot analysis of extracts from cos-7 cells (Lane 2), using MEF2C (Phospho-Ser396) Antibody. The lane on the left is treated with synthesized peptide.)

WB (Western Blot) (Western blot analysis of extracts from 3T3 cells, treated with starved (24hours), using MEF2C (Phospho-Ser396) antibody.)

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MEF2C (Phospho-Ser396), Polyclonal Antibody (Cat# AAA287681)

Full Name

MEF2C (Phospho-Ser396) Antibody

Gene Names

MEF2C; DEL5q14.3; C5DELq14.3

Applications

Immunohistochemistry, Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot) (The anti-Phospho-Rad9-S277 Pab is used in Western blot to detect Phospho-Rad9-S277 in Y79 tissue lysate)

Compare (5 max)

Phospho-RAD9 (S277), Polyclonal Antibody (Cat# AAA287884)

Full Name

Phospho-RAD9 (S277) Antibody

Gene Names

RAD9A; RAD9

Reactivity

Human (Predicted Reactivity: Monkey)

Applications

Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence analysis of A549 cells, using ACK1 (Phospho-Tyr284) antibody.)

IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human breast carcinoma tissue using ACK1 (Phospho-Tyr284) antibody.)

WB (Western Blot) (Western blot analysis of extracts from HepG2 cells, treated with EGF (200ng/ml, 30mins), using ACK1 (Phospho-Tyr284) antibody.)

Compare (5 max)

ACK1 (Phospho-Tyr284), Polyclonal Antibody (Cat# AAA290076)

Full Name

ACK1 (Phospho-Tyr284) Antibody

Gene Names

TNK2; ACK; ACK1; ACK-1; p21cdc42Hs

Reactivity

Human, mouse, rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

< >

<< Previous Next >>

DB (Dot Blot) (Dot blot analysis of anti-phospho-EphA2-pS897 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

WB (Western Blot) (Western blot analysis of EphA2 Antibody (pS897) Pab (RB21717) pre-incubated without(lane 1) and with(lane 2) FBS in HA cell line lysate. EphA2 Antibody (pS897) (arrow) was detected using the purified Pab(Kindly provided by Dr. John Wu).)

WB (Western Blot) (Western blot analysis of lysates from A431 cell line, untreated or treated with EGF, 100ng/ml, using phospho-EphA2 Antibody (pS897) (upper) or Beta-actin (lower).)

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Phospho-EphA2 (S897), Polyclonal Antibody (Cat# AAA290118)

Full Name

Phospho-EphA2 (S897) Antibody

Gene Names

EPHA2; ECK; CTPA; ARCC2; CTPP1; CTRCT6

Reactivity

Human (Predicted Reactivity: Monkey, Mouse)

Applications

Dot Blot, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

< >

<< Previous Next >>

IF (Immunofluorescence) (Confocal immunofluorescent analysis of Phospho-ABL1-Y245 Antibody with MCF-7 cell followed by Alexa Fluor 488-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).)

IHC (Immunohiostchemistry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot) (Western blot analysis of anti-ABL Pab in mouse colon tissue lysate (35ug/lane). ABL(arrow) was detected using the purified Pab.)

Compare (5 max)

Phospho-ABL1 (Y245), Polyclonal Antibody (Cat# AAA290132)

Full Name

Phospho-ABL1 (Y245) Antibody

Gene Names

ABL1; ABL; JTK7; p150; c-ABL; v-abl; c-ABL1; bcr/abl

Reactivity

Human, mouse

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

< >

<< Previous Next >>

IHC (Immunohistochemisry) (Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

WB (Western Blot) (The anti-Phospho-Rb-like-1-S975 Pab AAA290145 is used in Western blot to detect Phospho-Rb-like-1-S975 in A2058 tissue lysate)

WB (Western Blot) (All lanes : Anti-Phospho-Rb-like-1(S975) Antibody (upper) at 1:250 dilution + Hela whole cell lysates, Beta-actin (lower)Lane 1: G0-phase?serum-deprived 24h?Lane 2: S-phase?hydroxycarbamide?5mM, 24hLane 3: G2/M-phase?etoposide?5mM, 24hLane 4: M-phase, nocodazole, 150ng/ml 20hLysates/proteins at 20 ug per lane.SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilutionPredicted band size : 121 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)

Compare (5 max)

Phospho-Rb-like-1 (S975), Polyclonal Antibody (Cat# AAA290145)

Full Name

Phospho-Rb-like-1 (S975) Antibody

Gene Names

RBL1; PRB1; p107; CP107

Reactivity

Reactivity: Human
Predicted Reactivity: Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Peptide Affinity Purified Rabbit Polyclonal Antibody (Pab)

Pricing

$245/0.08 mL $485/0.4 mL $945/2x0.4 mL $1,385/3x0.4 mL $1,795/4x0.4 mL $2,040/5x0.4 mL

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<< Previous Next >>

DB (Dot Blot) (Dot blot analysis of anti-Phospho-SHP2-pY584 Phospho-specific Pab on nitrocellulose membrane. 50ng of Phospho-peptide or Non Phospho-peptide per dot were adsorbed. Antibody working concentrations are 0.5ug per ml.)

WB (Western Blot) (Western blot analysis of lysates from mouse NIH/3T3 cell line,untreated or treated with PDGF,using Phospho-SHP2-pY584 (upper) or Beta-actin (lower).)

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SHP2, Polyclonal Antibody (Cat# AAA290943)

Full Name

Phospho-SHP2 (Y584) Antibody

Gene Names

PTPN11; CFC; NS1; JMML; SHP2; BPTP3; PTP2C; METCDS; PTP-1D; SH-PTP2; SH-PTP3

Reactivity

Human

Applications

Dot Blot, Western Blot

Pricing

$480/0.1 mL $935/2x0.1 mL $1,370/3x0.1 mL $1,775/4x0.1 mL $2,000/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

Compare (5 max)

PR, Polyclonal Antibody (Cat# AAA293998)

Full Name

PR (phospho Ser294) Polyclonal Antibody

Gene Names

PGR; PR; NR3C3

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)

Compare (5 max)

CD5, Polyclonal Antibody (Cat# AAA294000)

Full Name

CD5 (phospho Tyr453) Polyclonal Antibody

Gene Names

CD5; T1; LEU1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

< >

<< Previous Next >>

IHC (Immunohiostchemistry) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)

Compare (5 max)

Cytokeratin 18, Polyclonal Antibody (Cat# AAA294001)

Full Name

Cytokeratin 18 (phospho Ser52) Polyclonal Antibody

Gene Names

KRT18; K18; CYK18

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

< >

<< Previous Next >>

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)

WB (Western Blot) (Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)

Compare (5 max)

DAPK3, Polyclonal Antibody (Cat# AAA294002)

Full Name

DAPK3 (phospho Thr265) Polyclonal Antibody

Gene Names

DAPK3; ZIP; ZIPK

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

What Are Phospho Antibodies?

Protein phosphorylation is a process where a phosphate group is added to certain amino acid residues of a protein – usually serine (S), threonine (T), or tyrosine (Y) - by enzymes called kinases. This process is integral in controlling cellular signaling, cellular growth, and other biological functions.

Our catalog includes a wide range of phospho-specific antibodies that can accurately detect this important marker. They perform strongly in widely-used laboratory applications such as Western blot, flow cytometry, immunohistochemistry, and immunofluorescence microscopy. We value your trust in us and are committed to providing top-quality products and services. All of our antibodies are guaranteed to work for the applications and species indicated on our website & associated product pages.

What Are The Key Applications of Phospho Antibodies?

1. Western Blotting

One of the first steps a researcher can take in utilizing these phospho-specific antibodies, is to check if the antibody works using a technique referred to as “Western blot”. For those unfamiliar, Western Blot aids in showing whether the protein that the antibody recognizes is appearing at the correct/expected size. These phospho-specific antibodies should also be able to detect changes in the target protein’s phosphorylation (on/off state) when cells are stimulated in certain ways.

2. Staining of Fixed Cells (Immunocytochemistry)

Another routine use of these phospho-specific antibodies, is to test if the antibody is able to demonstrate similar performance when used on fixed cells (intact cells that have been preserved) as it did in the Western blot tests. It is an important aspect in many cases to confirm that the antibody works in actual intact cell samples. Ideally, the method used for cellular fixation should be the same as what is used in pathology labs (like using 10% formalin). To check if the antibody works well in tissue sections (FFPE), researchers will often test it on fixed cells that are processed similar to tissue samples.

3. Specificity Tests Using Peptides

In order to make sure that the antibody is only binding to the right target:

• Laboratory technicians will mix the antibody with phospho-peptides (short segments of the protein containing the phosphate group modification).


• If the antibody signal disappears, it is confirmation that it is binding to the correct phosphorylated location.


• A more robust test is to use both the phosphorylated and non-phosphorylated (dephosphorylated) versions of the protein. The antibody should react only with the phosphorylated one.


• Another method sometimes utilized is to treat the sample with an enzyme, such as alkaline phosphatase, that specifically removes phosphate groups. If the antibody signal disappears after this, it also confirms specificity.

4. Genetic Confirmation

As a final step, scientists can genetically manipulate the nucleotide sequence and alter the target protein by removing the exact site where phosphorylation happens. If the antibody no longer appears to detect the modified protein, it is strong evidence supporting the antibody being specific for that phosphorylated site.

Why Buy Phospho Antibodies Through Us?

• The production laboratory adheres to strict and consistent protocols prior to releasing any of these phospho-specific antibodies:


• Standard methods and proper controls in all tests to ensure high quality.


• These antibodies are tested and validated in different cell types and species.


• High quality control criterion to ensure each batch is consistent, so you will obtain reliable results every time.

FAQ

1. What Are Phospho-Specific Antibodies?

Phospho-specific antibodies are made to detect proteins only when they have a phosphate group linked to a specific amino acid residue. This empowers scientists understand if a protein is "turned on" or active, based on its phosphorylation state.

2. How to Detect Phosphorylated Proteins in a Western Blot?

To find out if a protein is phosphorylated using Western blot:

• Use a phospho-specific antibody that binds only to the phosphorylated form of the protein.


• You can also use a “regular” antibody for the same amino acid sequence of the protein that the phospho-specific antibody is binding to (but in this case, this antibody will not bind if there is a phosphate group present) in order to compare how much of it is phosphorylated versus how much is non-phosphorylated (or “total” protein, if the “normal” antibody’s epitopes are non-phospho-site-specific).

3. How to Choose the Best Antibody?

Here are some simple tips to help you pick the right antibody:

• Know your target


• Match your sample characteristics


• Confirm the intended use is appropriate


• Check “host” and “type”


• Check the “quality” of the presented data/images


• Appraise whether the available validation meets your needs