Phospho Antibodies
Phospho-specific antibodies’ typical purpose is to enable researchers to detect changes in proteins. They will exclusively bind to the amino acid sequence on a protein that has been phosphorylated (which is both a physical & chemical change) and do not bind to the same amino acid sequence on said protein if it lacks said phosphorylation. This aids in being able to clearly see and understand the data produced from this particular protein modification.
Viewing 4000 -4050 of 5299 product results
Display
5 results per
page
10 results per
page
20 results per
page
50 results per
page
100 results
per page
200 results
per page
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)
Compare (5 max)
MKP-1/2 (phospho Ser296/318) Polyclonal Antibody
DUSP1; HVH1; MKP1; CL100; MKP-1; PTPN10
Immunohistochemistry, Western Blot
$485/0.1 mL
$945/2x0.1 mL
$1,385/3x0.1 mL
$1,795/4x0.1 mL
$2,040/5x0.1 mL
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)
Compare (5 max)
p47-phox (phospho Ser345) Polyclonal Antibody
NCF1; NCF1A; NOXO2; p47phox; SH3PXD1A
Immunohistochemistry, Western Blot
$485/0.1 mL
$945/2x0.1 mL
$1,385/3x0.1 mL
$1,795/4x0.1 mL
$2,040/5x0.1 mL
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)
Compare (5 max)
Cortactin (phospho Tyr466) Polyclonal Antibody
Human, Mouse, Rat, Monkey
Immunofluorescence, Immunohistochemistry, Western Blot
$485/0.1 mL
$945/2x0.1 mL
$1,385/3x0.1 mL
$1,795/4x0.1 mL
$2,040/5x0.1 mL
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)
Compare (5 max)
PKC delta (phospho Ser645) Polyclonal Antibody
PRKCD; MAY1; PKCD; nPKC-delta
Immunohistochemistry, Western Blot
$485/0.1 mL
$945/2x0.1 mL
$1,385/3x0.1 mL
$1,795/4x0.1 mL
$2,040/5x0.1 mL
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)
IHC (Immunohiostchemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)
Compare (5 max)
Adducin alpha/beta (phospho Ser726/713) Polyclonal Antibody
Immunofluorescence, Immunohistochemistry, Western Blot
$485/0.1 mL
$945/2x0.1 mL
$1,385/3x0.1 mL
$1,795/4x0.1 mL
$2,040/5x0.1 mL
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)
Compare (5 max)
c-Myc (phospho Ser62) Polyclonal Antibody
MYC; MRTL; c-Myc; bHLHe39
Immunofluorescence, Immunohistochemistry, Western Blot
$485/0.1 mL
$945/2x0.1 mL
$1,385/3x0.1 mL
$1,795/4x0.1 mL
$2,040/5x0.1 mL
IHC (Immunohistochemisry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)
Compare (5 max)
TH (phospho Ser19) Polyclonal Antibody
Immunofluorescence, Immunohistochemistry, Western Blot
$485/0.1 mL
$945/2x0.1 mL
$1,385/3x0.1 mL
$1,795/4x0.1 mL
$2,040/5x0.1 mL
IHC (Immunohistochemisry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)
Compare (5 max)
Akt (phospho Ser473) Polyclonal Antibody
AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA
Immunofluorescence, Immunohistochemistry, Western Blot
$485/0.1 mL
$945/2x0.1 mL
$1,385/3x0.1 mL
$1,795/4x0.1 mL
$2,040/5x0.1 mL
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)
IHC (Immunohiostchemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)
Compare (5 max)
Crk-L (phospho Tyr207) Polyclonal Antibody
Human, Mouse, Rat, Monkey
Immunofluorescence, Immunohistochemistry, Western Blot
$485/0.1 mL
$945/2x0.1 mL
$1,385/3x0.1 mL
$1,795/4x0.1 mL
$2,040/5x0.1 mL
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)
Compare (5 max)
EF-2 (phospho Thr56) Polyclonal Antibody
Immunofluorescence, Immunohistochemistry, Western Blot
$485/0.1 mL
$945/2x0.1 mL
$1,385/3x0.1 mL
$1,795/4x0.1 mL
$2,040/5x0.1 mL
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)
Compare (5 max)
Caldesmon (phospho Ser789) Polyclonal Antibody
CALD1; CDM; HCAD; LCAD; H-CAD; L-CAD; NAG22
Immunofluorescence, Immunohistochemistry, Western Blot
$485/0.1 mL
$945/2x0.1 mL
$1,385/3x0.1 mL
$1,795/4x0.1 mL
$2,040/5x0.1 mL
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)
Compare (5 max)
IRS-1 (phospho Ser323) Polyclonal Antibody
Human, Mouse, Rat, Monkey
Immunofluorescence, Immunohistochemistry, Western Blot
$485/0.1 mL
$945/2x0.1 mL
$1,385/3x0.1 mL
$1,795/4x0.1 mL
$2,040/5x0.1 mL
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)
Compare (5 max)
Splicing factor 1 (phospho Ser82) Polyclonal Antibody
SF1; BBP; MBBP; ZFM1; ZNF162; D11S636; ZCCHC25
Immunofluorescence, Immunohistochemistry, Western Blot
$485/0.1 mL
$945/2x0.1 mL
$1,385/3x0.1 mL
$1,795/4x0.1 mL
$2,040/5x0.1 mL
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/40000. Not yet tested in other applications.)
Compare (5 max)
Cytokeratin 8 (phospho Ser432) Polyclonal Antibody
KRT8; K8; KO; CK8; CK-8; CYK8; K2C8; CARD2
Immunofluorescence, Immunohistochemistry, Western Blot
$485/0.1 mL
$945/2x0.1 mL
$1,385/3x0.1 mL
$1,795/4x0.1 mL
$2,040/5x0.1 mL
IHC (Immunohiostchemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)
Compare (5 max)
HDAC6 (phospho Ser22) Polyclonal Antibody
Immunofluorescence, Immunohistochemistry, Western Blot
$485/0.1 mL
$945/2x0.1 mL
$1,385/3x0.1 mL
$1,795/4x0.1 mL
$2,040/5x0.1 mL
IHC (Immunohiostchemistry)
(Dilution: Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)
IHC (Immunohistochemistry)
(Dilution: Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)
Compare (5 max)
HMG-17 (phospho Ser29) Polyclonal Antibody
$485/0.1 mL
$945/2x0.1 mL
$1,385/3x0.1 mL
$1,795/4x0.1 mL
$2,040/5x0.1 mL
IHC (Immunohiostchemistry)
(Dilution: Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)
IHC (Immunohistochemistry)
(Dilution: Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)
Compare (5 max)
Huntingtin (phospho Ser421) Polyclonal Antibody
$485/0.1 mL
$945/2x0.1 mL
$1,385/3x0.1 mL
$1,795/4x0.1 mL
$2,040/5x0.1 mL
WB (Western Blot)
(Dilution: Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)
IHC (Immunohistochemistry)
(Dilution: Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)
Compare (5 max)
IP3R-I (phospho Ser1764) Polyclonal Antibody
ITPR1; IP3R; IP3R1; SCA15; SCA16; SCA29; INSP3R1
Immunohistochemistry, Western Blot
$485/0.1 mL
$945/2x0.1 mL
$1,385/3x0.1 mL
$1,795/4x0.1 mL
$2,040/5x0.1 mL
WB (Western Blot)
(Dilution: Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)
IHC (Immunohistochemistry)
(Dilution: Immunohistochemistry: 1/100 - 1/300. ELISA: 1/5000. Not yet tested in other applications.)
Compare (5 max)
TIRAP (phospho Tyr86) Polyclonal Antibody
TIRAP; Mal; wyatt; BACTS1; MyD88-2
Immunohistochemistry, Western Blot
$485/0.1 mL
$945/2x0.1 mL
$1,385/3x0.1 mL
$1,795/4x0.1 mL
$2,040/5x0.1 mL
IHC (Immunohiostchemistry)
(Dilution: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)
IHC (Immunohistochemistry)
(Dilution: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)
Compare (5 max)
DAPK1 (phospho Ser308) Polyclonal Antibody
Immunofluorescence, Immunohistochemistry
$485/0.1 mL
$945/2x0.1 mL
$1,385/3x0.1 mL
$1,795/4x0.1 mL
$2,040/5x0.1 mL
IHC (Immunohiostchemistry)
(Dilution: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)
IHC (Immunohistochemistry)
(Dilution: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)
Compare (5 max)
NMDAepsilon1/2 (phospho Tyr1246/1252) Polyclonal Antibody
GRIN2A; LKS; EPND; FESD; NR2A; GluN2A; NMDAR2A
Immunofluorescence, Immunohistochemistry
$485/0.1 mL
$945/2x0.1 mL
$1,385/3x0.1 mL
$1,795/4x0.1 mL
$2,040/5x0.1 mL
IHC (Immunohistochemistry)
(Dilution: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)
IHC (Immunohistochemisry)
(Dilution: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)
IHC (Immunohiostchemistry)
(Dilution: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)
IHC (Immunohistochemistry)
(Dilution: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)
Compare (5 max)
MAP-4 (phospho Ser696) Polyclonal Antibody
MAP4; MGC8617; DKFZp779A1753
Immunofluorescence, Immunohistochemistry
$485/0.1 mL
$945/2x0.1 mL
$1,385/3x0.1 mL
$1,795/4x0.1 mL
$2,040/5x0.1 mL
WB (Western Blot)
(Dilution: Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)
IF (Immunofluorescence)
(Dilution: Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)
Compare (5 max)
EDG-1 (phospho Thr236) Polyclonal Antibody
S1PR1; EDG1; S1P1; CD363; ECGF1; EDG-1; CHEDG1; D1S3362
Immunofluorescence, Western Blot
$485/0.1 mL
$945/2x0.1 mL
$1,385/3x0.1 mL
$1,795/4x0.1 mL
$2,040/5x0.1 mL
WB (Western Blot)
(Dilution: WB 1:500-2000, ELISA(peptide)1:5000-20000)
WB (Western Blot)
(Dilution: WB 1:500-2000, ELISA(peptide)1:5000-20000)
Compare (5 max)
CaMK2 (Phospho-Thr286) Antibody
$485/0.1 mL
$945/2x0.1 mL
$1,385/3x0.1 mL
$1,795/4x0.1 mL
$2,040/5x0.1 mL
Application Data
(Dilution: IHC-p 1:50-300)
IHC (Immunohistochemistry)
(Dilution: IHC-p 1:50-300)
Compare (5 max)
Phospho-Smad3 (S425) mouse Monoclonal Antibody (1D9)
SMAD3; LDS3; LDS1C; MADH3; JV15-2; HSPC193; HsT17436
$485/0.1 mL
$945/2x0.1 mL
$1,385/3x0.1 mL
$1,795/4x0.1 mL
$2,040/5x0.1 mL
IHC (Immunohiostchemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)
Compare (5 max)
4E-BP1 (phospho Thr69) Polyclonal Antibody
Immunohistochemistry, Western Blot
$485/0.1 mL
$945/2x0.1 mL
$1,385/3x0.1 mL
$1,795/4x0.1 mL
$2,040/5x0.1 mL
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)
Compare (5 max)
AP-1/Jun D (phospho Ser73/100) Polyclonal Antibody
Immunohistochemistry, Western Blot
$485/0.1 mL
$945/2x0.1 mL
$1,385/3x0.1 mL
$1,795/4x0.1 mL
$2,040/5x0.1 mL
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/20000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/20000. Not yet tested in other applications.)
Compare (5 max)
AP-1 (phospho Thr239) Polyclonal Antibody
Immunoprecipitation, Immunohistochemistry, Western Blot
$485/0.1 mL
$945/2x0.1 mL
$1,385/3x0.1 mL
$1,795/4x0.1 mL
$2,040/5x0.1 mL
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/20000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/20000. Not yet tested in other applications.)
Compare (5 max)
ATF-2 (phospho Ser112) Polyclonal Antibody
ATF2; HB16; CREB2; TREB7; CRE-BP1
Immunoprecipitation, Immunohistochemistry, Western Blot
$485/0.1 mL
$945/2x0.1 mL
$1,385/3x0.1 mL
$1,795/4x0.1 mL
$2,040/5x0.1 mL
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/20000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/20000. Not yet tested in other applications.)
Compare (5 max)
ATF-2 (phospho Thr71) Polyclonal Antibody
ATF2; HB16; CREB2; TREB7; CRE-BP1
Immunoprecipitation, Immunohistochemistry, Western Blot
$485/0.1 mL
$945/2x0.1 mL
$1,385/3x0.1 mL
$1,795/4x0.1 mL
$2,040/5x0.1 mL
IHC (Immunohistochemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)
IHC (Immunohiostchemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)
Compare (5 max)
C/EBP beta (phospho Thr235) Polyclonal Antibody
CEBPB; LAP; CRP2; TCF5; IL6DBP; NF-IL6; C/EBP-beta
Immunofluorescence, Immunohistochemistry, Western Blot
$485/0.1 mL
$945/2x0.1 mL
$1,385/3x0.1 mL
$1,795/4x0.1 mL
$2,040/5x0.1 mL
IHC (Immunohiostchemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)
Compare (5 max)
Caspase-6 (phospho Ser257) Polyclonal Antibody
Immunofluorescence, Immunohistochemistry, Western Blot
$485/0.1 mL
$945/2x0.1 mL
$1,385/3x0.1 mL
$1,795/4x0.1 mL
$2,040/5x0.1 mL
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.)
Compare (5 max)
Caveolin-1 (phospho Tyr14) Polyclonal Antibody
CAV1; CGL3; BSCL3; VIP21; MSTP085
$485/0.1 mL
$945/2x0.1 mL
$1,385/3x0.1 mL
$1,795/4x0.1 mL
$2,040/5x0.1 mL
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)
Compare (5 max)
Cdk1/2/3 (phospho Thr14) Polyclonal Antibody
CDK1; CDC2; CDC28A; P34CDC2
Human, Mouse, Rat, Monkey
Immunofluorescence, Western Blot
$485/0.1 mL
$945/2x0.1 mL
$1,385/3x0.1 mL
$1,795/4x0.1 mL
$2,040/5x0.1 mL
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/10000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/10000. Not yet tested in other applications.)
Compare (5 max)
c-Myc (phospho Thr58) Polyclonal Antibody
MYC; MRTL; c-Myc; bHLHe39
Immunoprecipitation, Immunohistochemistry, Western Blot
$485/0.1 mL
$945/2x0.1 mL
$1,385/3x0.1 mL
$1,795/4x0.1 mL
$2,040/5x0.1 mL
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)
Compare (5 max)
Cyclin E1 (phospho Thr395) Polyclonal Antibody
Immunohistochemistry, Western Blot
$485/0.1 mL
$945/2x0.1 mL
$1,385/3x0.1 mL
$1,795/4x0.1 mL
$2,040/5x0.1 mL
WB (Western Blot)
(Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: IF: 1:50-200 Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)
Compare (5 max)
eIF2alpha (phospho Ser51) Polyclonal Antibody
EIF2S1; EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha
Immunofluorescence, Immunohistochemistry, Western Blot
$485/0.1 mL
$945/2x0.1 mL
$1,385/3x0.1 mL
$1,795/4x0.1 mL
$2,040/5x0.1 mL
IHC (Immunohiostchemistry)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)
Compare (5 max)
FoxO4 (phospho Ser197) Polyclonal Antibody
Immunohistochemistry, Western Blot
$485/0.1 mL
$945/2x0.1 mL
$1,385/3x0.1 mL
$1,795/4x0.1 mL
$2,040/5x0.1 mL
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. ELISA: 1/20000. Not yet tested in other applications.)
Compare (5 max)
GATA-1 (phospho Ser310) Polyclonal Antibody
GATA1; GF1; GF-1; NFE1; XLTT; ERYF1; XLANP; XLTDA; GATA-1
Human, Mouse, Rat, Monkey
$485/0.1 mL
$945/2x0.1 mL
$1,385/3x0.1 mL
$1,795/4x0.1 mL
$2,040/5x0.1 mL
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/20000. Not yet tested in other applications.)
Compare (5 max)
HDAC5 (phospho Ser498) Polyclonal Antibody
Immunofluorescence, Immunohistochemistry, Western Blot
$485/0.1 mL
$945/2x0.1 mL
$1,385/3x0.1 mL
$1,795/4x0.1 mL
$2,040/5x0.1 mL
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)
Compare (5 max)
IKKalpha/beta (phospho Ser180/181) Polyclonal Antibody
CHUK; IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha
Immunohistochemistry, Western Blot
$485/0.1 mL
$945/2x0.1 mL
$1,385/3x0.1 mL
$1,795/4x0.1 mL
$2,040/5x0.1 mL
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/20000. Not yet tested in other applications.)
Compare (5 max)
IRS-1 (phospho Ser1101) Polyclonal Antibody
Human, Mouse, Rat, Monkey
Immunohistochemistry, Western Blot
$485/0.1 mL
$945/2x0.1 mL
$1,385/3x0.1 mL
$1,795/4x0.1 mL
$2,040/5x0.1 mL
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. ELISA: 1/10000. Not yet tested in other applications.)
Compare (5 max)
MEF-2D (phospho Ser444) Polyclonal Antibody
Immunohistochemistry, Western Blot
$485/0.1 mL
$945/2x0.1 mL
$1,385/3x0.1 mL
$1,795/4x0.1 mL
$2,040/5x0.1 mL
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/10000. Not yet tested in other applications.)
WB (Western Blot)
(Dilution: Western Blot: 1/500 - 1/2000. Immunohistochemistry: 1/100 - 1/300. Immunoprecipitation: 2-5 ug/mg lysate. ELISA: 1/10000. Not yet tested in other applications.)
Compare (5 max)
MEK-2 (phospho Thr394) Polyclonal Antibody
MAP2K2; CFC4; MEK2; MKK2; MAPKK2; PRKMK2
Immunoprecipitation, Immunohistochemistry, Western Blot
$485/0.1 mL
$945/2x0.1 mL
$1,385/3x0.1 mL
$1,795/4x0.1 mL
$2,040/5x0.1 mL
Application Data
(Dilution: IHC 1:100-200)
Application Data
(Dilution: IHC 1:100-200)
Application Data
(Dilution: IHC 1:100-200)
IHC (Immunohistochemistry)
(Dilution: IHC: 1:100-200)
Compare (5 max)
CaMKIIbeta/gamma/delta (Phospho Thr287) Monoclonal Antibody (4H2)
CAMK2B; CAM2; CAMK2; CAMKB
$485/0.1 mL
$945/2x0.1 mL
$1,385/3x0.1 mL
$1,795/4x0.1 mL
$2,040/5x0.1 mL
Standard Curve (Sample)
Compare (5 max)
Bovine Phospho Tou protein ELISA Kit
$595/48-Strip-Wells
$845/96-Strip-Wells
$1,640/2x96-Strip-Wells
$3,715/5x96-Strip-Wells
$7,005/10x96-Strip-Wells
ICC (Immunocytochemistry)
(Immunocytochemical labeling of Talin phosphorylation relative to F-actin in chick fibroblasts. The cells were labeled with rabbit polyclonal Talin (Ser-425) antibody (TP4171), then the antibody was detected using appropriate secondary antibody (Green). This labeling is compared to F-actin staining (Red, Left). (Image provided by Dr. Gianluca Gallo at Drexel University).)
WB (Western Blot)
(Western blot of rat PC12 cells stimulated with Calyculin A (100nM) for 30 min (lanes 1-4). The blot was treated with lambda phosphatase (lanes 2 & 4), then probed with mouse monoclonal anti-Talin (8D4) (lanes 1 & 2) or rabbit polyclonal anti-Talin (Ser-425) (lanes 3 & 4) antibodies.)
Compare (5 max)
Talin (Ser-425), phospho-specific Antibody
Human, Rat, Mouse, Chicken, Fish
Immunocytochemistry, Western Blot
$505/0.1 mL
$985/2x0.1 mL
$1,440/3x0.1 mL
$1,875/4x0.1 mL
$2,100/5x0.1 mL
What Are Phospho Antibodies?
Protein phosphorylation is a process where a phosphate group is added to certain amino acid residues of a protein – usually serine (S), threonine (T), or tyrosine (Y) - by enzymes called kinases. This process is integral in controlling cellular signaling, cellular growth, and other biological functions.
Our catalog includes a wide range of phospho-specific antibodies that can accurately detect this important marker. They perform strongly in widely-used laboratory applications such as Western blot, flow cytometry, immunohistochemistry, and immunofluorescence microscopy. We value your trust in us and are committed to providing top-quality products and services. All of our antibodies are guaranteed to work for the applications and species indicated on our website & associated product pages.
What Are The Key Applications of Phospho Antibodies?
1. Western Blotting
One of the first steps a researcher can take in utilizing these phospho-specific antibodies, is to check if the antibody works using a technique referred to as “Western blot”. For those unfamiliar, Western Blot aids in showing whether the protein that the antibody recognizes is appearing at the correct/expected size. These phospho-specific antibodies should also be able to detect changes in the target protein’s phosphorylation (on/off state) when cells are stimulated in certain ways.
2. Staining of Fixed Cells (Immunocytochemistry)
Another routine use of these phospho-specific antibodies, is to test if the antibody is able to demonstrate similar performance when used on fixed cells (intact cells that have been preserved) as it did in the Western blot tests. It is an important aspect in many cases to confirm that the antibody works in actual intact cell samples. Ideally, the method used for cellular fixation should be the same as what is used in pathology labs (like using 10% formalin). To check if the antibody works well in tissue sections (FFPE), researchers will often test it on fixed cells that are processed similar to tissue samples.
3. Specificity Tests Using Peptides
In order to make sure that the antibody is only binding to the right target:
Laboratory technicians will mix the antibody with phospho-peptides (short segments of the protein containing the phosphate group modification).
If the antibody signal disappears, it is confirmation that it is binding to the correct phosphorylated location.
A more robust test is to use both the phosphorylated and non-phosphorylated (dephosphorylated) versions of the protein. The antibody should react only with the phosphorylated one.
Another method sometimes utilized is to treat the sample with an enzyme, such as alkaline phosphatase, that specifically removes phosphate groups. If the antibody signal disappears after this, it also confirms specificity.
4. Genetic Confirmation
As a final step, scientists can genetically manipulate the nucleotide sequence and alter the target protein by removing the exact site where phosphorylation happens. If the antibody no longer appears to detect the modified protein, it is strong evidence supporting the antibody being specific for that phosphorylated site.
Why Buy Phospho Antibodies Through Us?
The production laboratory adheres to strict and consistent protocols prior to releasing any of these phospho-specific antibodies:
Standard methods and proper controls in all tests to ensure high quality.
These antibodies are tested and validated in different cell types and species.
High quality control criterion to ensure each batch is consistent, so you will obtain reliable results every time.
FAQ
1. What Are Phospho-Specific Antibodies?
Phospho-specific antibodies are made to detect proteins only when they have a phosphate group linked to a specific amino acid residue. This empowers scientists understand if a protein is "turned on" or active, based on its phosphorylation state.
2. How to Detect Phosphorylated Proteins in a Western Blot?
To find out if a protein is phosphorylated using Western blot:
Use a phospho-specific antibody that binds only to the phosphorylated form of the protein.
You can also use a “regular” antibody for the same amino acid sequence of the protein that the phospho-specific antibody is binding to (but in this case, this antibody will not bind if there is a phosphate group present) in order to compare how much of it is phosphorylated versus how much is non-phosphorylated (or “total” protein, if the “normal” antibody’s epitopes are non-phospho-site-specific).
3. How to Choose the Best Antibody?
Here are some simple tips to help you pick the right antibody:
Know your target
Match your sample characteristics
Confirm the intended use is appropriate
Check “host” and “type”
Check the “quality” of the presented data/images
Appraise whether the available validation meets your needs