Phospho Antibodies

Phospho-specific antibodies’ typical purpose is to enable researchers to detect changes in proteins. They will exclusively bind to the amino acid sequence on a protein that has been phosphorylated (which is both a physical & chemical change) and do not bind to the same amino acid sequence on said protein if it lacks said phosphorylation. This aids in being able to clearly see and understand the data produced from this particular protein modification.

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IHC (Immunohiostchemistry) (Dilution: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)

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NMDAepsilon1/2, Polyclonal Antibody (Cat# AAA294139)

Full Name

NMDAepsilon1/2 (phospho Tyr1246/1252) Polyclonal Antibody

Gene Names

GRIN2A; LKS; EPND; FESD; NR2A; GluN2A; NMDAR2A

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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IHC (Immunohistochemistry) (Dilution: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

IHC (Immunohistochemisry) (Dilution: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

IHC (Immunohiostchemistry) (Dilution: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

IHC (Immunohistochemistry) (Dilution: Immunohistochemistry: 1/100 - 1/300. Immunofluorescence: 1/200 - 1/1000. ELISA: 1/5000. Not yet tested in other applications.)

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MAP-4, Polyclonal Antibody (Cat# AAA294141)

Full Name

MAP-4 (phospho Ser696) Polyclonal Antibody

Gene Names

MAP4; MGC8617; DKFZp779A1753

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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WB (Western Blot) (Dilution: Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)

IF (Immunofluorescence) (Dilution: Immunofluorescence: 1/200 - 1/1000. ELISA: 1/10000. Not yet tested in other applications.)

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EDG-1, Polyclonal Antibody (Cat# AAA294142)

Full Name

EDG-1 (phospho Thr236) Polyclonal Antibody

Gene Names

S1PR1; EDG1; S1P1; CD363; ECGF1; EDG-1; CHEDG1; D1S3362

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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WB (Western Blot) (Dilution: WB 1:500-2000, ELISA(peptide)1:5000-20000)

WB (Western Blot) (Dilution: WB 1:500-2000, ELISA(peptide)1:5000-20000)

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CaMK2, Polyclonal Antibody (Cat# AAA294148)

Full Name

CaMK2 (Phospho-Thr286) Antibody

Gene Names

CAMK2A; CAMKA

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Pricing

$485/0.1 mL $945/2x0.1 mL $1,385/3x0.1 mL $1,795/4x0.1 mL $2,040/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded rat hippocampal region tissue from a model with Alzheimer)

WB (Western Blot) (Western blot analysis of extracts from mouse brain tissue using Tau(Phospho-Ser396) Antibody and the same antibody preincubated with blocking peptide.)

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Tau, Polyclonal Antibody (Cat# AAA301111)

Full Name

Tau (Phospho-Ser396) Antibody

Gene Names

MAPT; TAU; MSTD; PPND; DDPAC; MAPTL; MTBT1; MTBT2; FTDP-17; PPP1R103

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

WB (Western Blot) (Western blot analysis of VEC using p-cPLA2 (S505) antibody.)

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cPLA2, Polyclonal Antibody (Cat# AAA301124)

Full Name

cPLA2 (Phospho-Ser505) Polyclonal Antibody

Gene Names

PLA2G4A; cPLA2; PLA2G4; cPLA2-alpha

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using 4E-BP1(Phospho-Thr45) Antibody (left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from Hela cells using 4E-BP1(Phospho-Thr45) Antibody and the same antibody preincubated with blocking peptide.)

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4E-BP1, Polyclonal Antibody (Cat# AAA301172)

Full Name

4E-BP1 (Phospho-Thr45) Antibody

Gene Names

EIF4EBP1; BP-1; 4EBP1; 4E-BP1; PHAS-I

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi

Pricing

$280/0.05 mL $340/0.1 mL $655/2x0.1 mL $955/3x0.1 mL $1,235/4x0.1 mL $1,330/5x0.1 mL

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IP (Immunoprecipitation) (Detection of human Phospho-MEK1 (T286) by immunoprecipitation (IP). Samples: 50 ug HeLa whole cell lysate (WCL) from mock treated (-) or nocodazole treated (+) cells. 1 mg of HeLa WCL mock treated (-) or nocodazole treated (+) and immunoprecipitated; 20% of the IP was loaded. Antibodies: Affinity purified goat anti-phospho MEK1 (T286) AAA212238 was used for IP (6ug/1mg lysate). To detect total MEK1, Rabbit anti-MEK1 (BL8445) was used for western blot (WB) at 1 ug/ml. Detection: Chemiluminescence with exposure time of 30 seconds.)

IHC (Immunohiostchemistry) (Detection of mouse Phospho-MEK1 (T286) by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified goat anti-phospho MEK1 (T286) (Cat. No. AAA212238 lot 1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)

IHC (Immunohistochemistry) (Detection of human Phospho-MEK1 (T286) by immunohistochemistry. Sample: FFPE section of human breast carcinoma. Antibody: Affinity purified goat anti-phospho MEK1 (T286) (Cat. No. AAA212238 lot 1) used at a dilution of 1:1,000 (1ug/ml). Detection: DAB)

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MEK1, Polyclonal Antibody (Cat# AAA212238)

Full Name

Goat anti-Phospho MEK1 (T286) Antibody, Affinity Purified

Gene Names

MAP2K1; MEK1; MKK1; MAPKK1; PRKMK1

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunoprecipitation

Purity

Antigen Affinity Purified

Pricing

$255/0.01 mg $635/0.1 mg $940/5x0.01 mg $1,230/2x0.1 mg $1,800/3x0.1 mg $2,335/4x0.1 mg $2,630/5x0.1 mg

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of HSP27 (pS15) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of HSP27 (pS15) expression in HeLa Anisomycin-treated (A), MCF7 (B) whole cell lysates.)

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HSP27 (pS15), Polyclonal Antibody (Cat# AAA104661)

Full Name

Anti-HSP27 (pS15) Phospho Antibody

Gene Names

HSPB1; CMT2F; HMN2B; HSP27; HSP28; Hsp25; SRP27; HS.76067; HEL-S-102

Reactivity

Human, Bovine, Monkey

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$240/0.03 mL $275/0.05 mL $345/0.1 mL $475/0.2 mL $925/2x0.2 mL $1,355/3x0.2 mL $1,755/4x0.2 mL $1,920/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of S6K1 (pT421) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of S6K1 (pT421) expression in MDAMB231 insulin-treated (A), Jurkat (B), HeLa (C), NIH3T3 (D), PC12 (E) whole cell lysates.)

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S6K1 (pT421), Polyclonal Antibody (Cat# AAA105101)

Full Name

Anti-S6K1 (pT421) Phospho Antibody

Gene Names

RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha

Reactivity

Human, Mouse, Rat, Bovine, Rabbit

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$240/0.03 mL $275/0.05 mL $345/0.1 mL $475/0.2 mL $925/2x0.2 mL $1,355/3x0.2 mL $1,755/4x0.2 mL $1,920/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of Ezrin (pT567) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of Ezrin (pT567) expression in HeLa (A), A431 (B), SHSY5Y (C) whole cell lysates.)

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Ezrin (pT567), Polyclonal Antibody (Cat# AAA105130)

Full Name

Anti-Ezrin (pT567) Phospho Antibody

Gene Names

EZR; CVL; CVIL; VIL2; HEL-S-105

Reactivity

Human, Mouse, Rat, Bovine, Chicken, Pig, Zebrafish

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$240/0.03 mL $275/0.05 mL $345/0.1 mL $475/0.2 mL $925/2x0.2 mL $1,355/3x0.2 mL $1,755/4x0.2 mL $1,920/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescent analysis of LCK (pY393) staining in MCF7 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)

WB (Western Blot) (Western blot analysis of LCK (pY393) expression in MCF7 TNFa-treated (A), mouse liver (B), rat liver (C) whole cell lysates.)

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LCK (pY393), Polyclonal Antibody (Cat# AAA105246)

Full Name

Anti-LCK (pY393) Phospho Antibody

Gene Names

LCK; LSK; YT16; IMD22; p56lck; pp58lck

Reactivity

Human, Mouse, Rat, Bovine, Chicken, Pig, Sheep

Applications

Immunocytochemistry, Immunofluorescence, Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$240/0.03 mL $275/0.05 mL $345/0.1 mL $475/0.2 mL $925/2x0.2 mL $1,355/3x0.2 mL $1,755/4x0.2 mL $1,920/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescent analysis of ETS1 (pT38) staining in Jurkat cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of ETS1 (pT38) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of ETS1 (pT38) expression in LO2 (A), Jurkat (B), HepG2 (C), NIH3T3 (D), rat liver (E) whole cell lysates.)

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ETS1 (pT38), Polyclonal Antibody (Cat# AAA105258)

Full Name

Anti-ETS1 (pT38) Phospho Antibody

Gene Names

ETS1; p54; ETS-1; EWSR2

Reactivity

Human, Mouse, Rat, Bovine, Chicken, Dog, Pig, Rabbit

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$240/0.03 mL $275/0.05 mL $345/0.1 mL $475/0.2 mL $925/2x0.2 mL $1,355/3x0.2 mL $1,755/4x0.2 mL $1,920/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of IKK beta (pY188) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of IKK beta (pY188) expression in HeLa LPS-treated (A), Raw264.7 LPS-treated (B), PC12 LPS-treated (C) whole cell lysates.)

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IKK beta (pY188), Polyclonal Antibody (Cat# AAA104734)

Full Name

Anti-IKK beta (pY188) Phospho Antibody

Gene Names

IKBKB; IKK2; IKKB; IMD15; NFKBIKB; IKK-beta

Reactivity

Human, Mouse, Rat, Bovine, Pig

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$240/0.03 mL $275/0.05 mL $345/0.1 mL $475/0.2 mL $925/2x0.2 mL $1,355/3x0.2 mL $1,755/4x0.2 mL $1,920/5x0.2 mL

WB (Western Blot)

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Phospho-PDE5A, Polyclonal Antibody (Cat# AAA76028)

Full Name

Phospho-PDE5A Antibody

Gene Names

PDE5A; CN5A; PDE5; CGB-PDE

Reactivity

Bovine, Human, Mouse, Pig, Rat

Applications

Western Blot, Immunoprecipitation, Immunohistochemistry, Immunocytochemistry

Pricing

$625/0.1 mg $1,210/2x0.1 mg $1,765/3x0.1 mg $2,295/4x0.1 mg $2,575/5x0.1 mg

WB (Western Blot)

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Phospho-MERTK, Polyclonal Antibody (Cat# AAA75659)

Full Name

Phospho-MERTK Antibody

Gene Names

Mertk; rdy

Reactivity

Human, Monkey, Mouse, Rat

Applications

Western Blot, Immunohistochemistry, Immunofluorescence, Immunocytochemistry

Pricing

$625/0.1 mg $1,210/2x0.1 mg $1,765/3x0.1 mg $2,295/4x0.1 mg $2,575/5x0.1 mg

WB (Western Blot) (Western blot analysis of extracts from 293 cells. Jurkat cells and NIH/3T3 cells all treated with Paclitaxel, using BCL2 (Phospho-Ser70) antibody.)

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BCL2, Antibody (Cat# AAA108866)

Full Name

BCL2 (Phospho-Ser70) Antibody

Gene Names

BCL2; Bcl-2; PPP1R50

Reactivity

Mouse, Rat

Applications

Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human brain tissue using FER (Phospho-Tyr402) antibody. Western blot analysis of extracts from Jurkat cells, treated with starved (24hours), using FER (Phospho-Tyr402) antibody.)

Application Data (P-peptide-+)

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FER, Antibody (Cat# AAA108988)

Full Name

FER (Phospho-Tyr402) Antibody

Gene Names

FER; TYK3; PPP1R74; p94-Fer

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF405M) $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (AP) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC)

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IF (Immunofluorescence) (Immunofluorescent analysis of MKK4 (pT261) staining in NIH3T3 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of MKK4 (pT261) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of MKK4 (pT261) expression in NIH3T3 PDGF-treated (A) whole cell lysates.)

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MKK4 (pT261), Polyclonal Antibody (Cat# AAA104286)

Full Name

Anti-MKK4 (pT261) Phospho Antibody

Gene Names

MAP2K4; JNKK; MEK4; MKK4; SEK1; SKK1; JNKK1; SERK1; MAPKK4; PRKMK4; SAPKK1; SAPKK-1

Reactivity

Human, Mouse, Rat, Bovine, Zebrafish

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$240/0.03 mL $275/0.05 mL $345/0.1 mL $475/0.2 mL $925/2x0.2 mL $1,355/3x0.2 mL $1,755/4x0.2 mL $1,920/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of PLC gamma 2 (pY753) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of PLC gamma 2 (pY753) expression in Hela TNFa-treated (A), MCF7 (B), Raw264.7 TNFa-treated (C), mouse kidney (D), rat kidney (E) whole cell lysates.)

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PLC gamma 2 (pY753), Polyclonal Antibody (Cat# AAA104531)

Full Name

Anti-PLC gamma 2 (pY753) Phospho Antibody

Gene Names

PLCG2; FCAS3; APLAID; PLC-IV; PLC-gamma-2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$240/0.03 mL $275/0.05 mL $345/0.1 mL $475/0.2 mL $925/2x0.2 mL $1,355/3x0.2 mL $1,755/4x0.2 mL $1,920/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of IKK gamma (pS85) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of IKK gamma (pS85) expression in THP1 LPS-treated (A), Raw264.7 LPS-treated (B), rat brain (C) whole cell lysates.)

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IKK gamma (pS85), Polyclonal Antibody (Cat# AAA104545)

Full Name

Anti-IKK gamma (pS85) Phospho Antibody

Gene Names

IKBKG; IP; IP1; IP2; FIP3; IPD2; NEMO; FIP-3; Fip3p; IMD33; AMCBX1; ZC2HC9; IKK-gamma

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$240/0.03 mL $275/0.05 mL $345/0.1 mL $475/0.2 mL $925/2x0.2 mL $1,355/3x0.2 mL $1,755/4x0.2 mL $1,920/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of DOK2 (pY299) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of DOK2 (pY299) expression in A549 Insulin-treated (A), HepG2 (B) whole cell lysates.)

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DOK2 (pY299), Polyclonal Antibody (Cat# AAA104314)

Full Name

Anti-DOK2 (pY299) Phospho Antibody

Gene Names

DOK2; p56DOK; p56dok-2

Reactivity

Human

Applications

Immunoprecipitation, Immunohistochemistry, Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$240/0.03 mL $275/0.05 mL $345/0.1 mL $475/0.2 mL $925/2x0.2 mL $1,355/3x0.2 mL $1,755/4x0.2 mL $1,920/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescent analysis of S6K1 (pS418) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of S6K1 (pS418) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of S6K1 (pS418) expression in NIH3T3 (A), HeLa (B), KNRK (C) whole cell lysates.)

Compare (5 max)

S6K1 (pS418), Polyclonal Antibody (Cat# AAA104336)

Full Name

Anti-S6K1 (pS418) Phospho Antibody

Gene Names

RPS6KB1; S6K; PS6K; S6K1; STK14A; p70-S6K; p70 S6KA; p70-alpha; S6K-beta-1; p70(S6K)-alpha

Reactivity

Human, Mouse, Rat, Bovine, Chicken, Dog, Pig, Rabbit

Applications

Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$240/0.03 mL $275/0.05 mL $345/0.1 mL $475/0.2 mL $925/2x0.2 mL $1,355/3x0.2 mL $1,755/4x0.2 mL $1,920/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescent analysis of p53 (pS15) staining in PC12 cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of p53 (pS15) staining in human breast cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of p53 (pS15) expression in HeLa UV-treated (A), PC12 (B) whole cell lysates.)

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p53 (pS15), Polyclonal Antibody (Cat# AAA104614)

Full Name

Anti-p53 (pS15) Phospho Antibody

Gene Names

TP53; P53; BCC7; LFS1; TRP53

Reactivity

Human, Rat

Applications

Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$240/0.03 mL $275/0.05 mL $345/0.1 mL $475/0.2 mL $925/2x0.2 mL $1,355/3x0.2 mL $1,755/4x0.2 mL $1,920/5x0.2 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of c-CBL (pY674) staining in human cervical cancer formalin fixed paraffin embedded tissue section. The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0). The section was then incubated with the antibody at room temperature and detected using an HRP conjugated compact polymer system. DAB was used as the chromogen. The section was then counterstained with haematoxylin and mounted with DPX.)

WB (Western Blot) (Western blot analysis of c-CBL (pY674) expression in HeLa EGF-treated (A), mouse liver (B), rat liver (C) whole cell lysates.)

Compare (5 max)

c-CBL (pY674), Polyclonal Antibody (Cat# AAA104619)

Full Name

Anti-c-CBL (pY674) Phospho Antibody

Gene Names

CBL; CBL2; NSLL; C-CBL; RNF55; FRA11B

Reactivity

Human, Mouse, Rat, Chicken

Applications

Immunohistochemistry, Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$240/0.03 mL $275/0.05 mL $345/0.1 mL $475/0.2 mL $925/2x0.2 mL $1,355/3x0.2 mL $1,755/4x0.2 mL $1,920/5x0.2 mL

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IF (Immunofluorescence) (Immunofluorescent analysis of Alpha-adducin (pS726/713) staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).)

WB (Western Blot) (Western blot analysis of Alpha-adducin (pS726/713) expression in HeLa (A), SP2/0 (B), PC12 (C) whole cell lysates.)

Compare (5 max)

Alpha-adducin, Polyclonal Antibody (Cat# AAA106184)

Full Name

Anti-Alpha-adducin (pS726/713) Phospho Antibody

Gene Names

ADD1; ADDA

Reactivity

Human, Mouse, Rat, Porcine

Applications

Immunoprecipitation, Immunocytochemistry, Immunofluorescence, Western Blot

Purity

The antibody was purified by immunogen affinity chromatography.

Pricing

$240/0.03 mL $275/0.05 mL $345/0.1 mL $475/0.2 mL $925/2x0.2 mL $1,355/3x0.2 mL $1,755/4x0.2 mL $1,920/5x0.2 mL

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WB (Western Blot) (P-peptide-+ Western blot analysis of extracts from HepG2 cells, treated with Adriamycin (0.5uM, 5hours), using Ataxin 1 (Phospho-Ser776) antibody. Immunofluorescence analysis of NIH/3T3 cells, using Ataxin 1 (Phospho-Ser776) antibody.)

Application Data

Compare (5 max)

Ataxin 1, Antibody (Cat# AAA111520)

Full Name

Ataxin 1 (Phospho-Ser776) Antibody

Gene Names

ATXN1; ATX1; SCA1; D6S504E

Reactivity

Human, Mouse

Applications

Immunofluorescence, Western Blot

Purity

Affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific phosphopeptide. The antibody against non-phosphopeptide was removed by chromatography using non-phosphopeptide corresponding to the phosphorylation site.

Pricing

$390/0.1 mg $580/0.1 mL (AF568) $580/0.1 mL (AF555) $580/0.1 mL (AF488) $580/0.1 mL (AF350) $580/0.1 mL (FITC) $580/0.1 mL (Biotin) $580/0.1 mL (AF405M) $580/0.1 mL (AF635) $580/0.1 mL (AF610) $580/0.1 mL (AF405S) $580/0.1 mL (AF405L) $580/0.1 mL (AF546) $580/0.1 mL (AF680) $580/0.1 mL (AF647) $580/0.1 mL (AF594) $580/0.1 mL (HRP) $580/0.1 mL (AF750) $710/0.1 mL (PE) $710/0.1 mL (Cy7) $710/0.1 mL (Cy5.5) $710/0.1 mL (Cy5) $710/0.1 mL (Cy3) $710/0.1 mL (APC) $710/0.1 mL (AP)

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WB (Western Blot) (HepG2 cells were subjected to SDS PAGE followed by western blot with AAA249539(Phospho-GSK3B (Ser9) antibody) at dilution of 1:1000)

IF (Immunofluorescence) (Immunofluorescent analysis of HepG2 cells using AAA249539(Phospho-GSK3B (Ser9) antibody) at dilution of 1:50.)

IHC (Immunohistochemistry) (Immunohistochemistry of paraffin-embedded human liver cancer tissue slide using AAA249539(Phospho-GSK3B (Ser9) Antibody) at dilution of 1:100)

Compare (5 max)

Phospho-GSK3B, Monoclonal Antibody (Cat# AAA249539)

Full Name

anti-Phospho-GSK3B (Ser9) antibody

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

>=95% as determined by SDS-PAGE. Protein A+G purification

Pricing

$390/0.1 mg $755/2x0.1 mg $1,105/3x0.1 mg $1,430/4x0.1 mg $1,520/5x0.1 mg

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Jun(Phospho-Ser243) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from sorbitol-treated 293, Serum-treated C2C12 and UV-treated HepG2 cells using c-Jun(Phospho-Ser243) Antibody.)

Compare (5 max)

JUN, Polyclonal Antibody (Cat# AAA243044)

Full Name

Phospho-JUN (Ser243) Antibody

Gene Names

JUN; AP1; AP-1; c-Jun

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF2(Phospho-Thr71 or 53) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from HUVEC cells untreated or treated with Anisomycin using ATF2 (Phospho-Thr71 or 53) Antibody.)

Compare (5 max)

ATF2, Polyclonal Antibody (Cat# AAA243049)

Full Name

Phospho-ATF2 (Thr71 or 53) Antibody

Gene Names

ATF2; HB16; CREB2; TREB7; CRE-BP1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using ATF2(Phospho-Ser112 or 94) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from Huvec cells untreated or treated with Anisomycin using ATF2(Phospho-Ser112 or 94) Antibody.)

Compare (5 max)

ATF2, Polyclonal Antibody (Cat# AAA243050)

Full Name

Phospho-ATF2 (Ser112 or 94) Antibody

Gene Names

ATF2; HB16; CREB2; TREB7; CRE-BP1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using PKD/PKCm(Phospho-Ser738) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from MCF cells untreated or treated with PMA using PKD/PKCm(Phospho-Ser738) Antibody.)

Compare (5 max)

PRKD1, Polyclonal Antibody (Cat# AAA243068)

Full Name

Phospho-PRKD1 (Ser738) Antibody

Gene Names

PRKD1; PKD; PKCM; PRKCM; PKC-MU

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using EGFR(Phospho-Ser1070) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from HUVEC cells untreated or treated with EGF using EGFR(Phospho-Ser1070) Antibody.)

Compare (5 max)

EGFR, Polyclonal Antibody (Cat# AAA243069)

Full Name

Phospho-EGFR (Ser1070) Antibody

Gene Names

EGFR; ERBB; HER1; mENA; ERBB1; PIG61

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using CDC2(Phospho-Thr161) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from Hela cells untreated(lane 1) or treated with UV(lane 2) using CDC2(Phospho-Thr161) Antibody.)

Compare (5 max)

CDK1, Polyclonal Antibody (Cat# AAA243092)

Full Name

Phospho-CDK1 (Thr161) Antibody

Gene Names

CDK1; CDC2; CDC28A; P34CDC2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using cdc25A(Phospho-Ser76) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from Hela cells untreated or treated with TNF-a and CA using cdc25A(Phospho-Ser76) Antibody.)

Compare (5 max)

CDC25A, Polyclonal Antibody (Cat# AAA243093)

Full Name

Phospho-CDC25A (Ser76) Antibody

Gene Names

CDC25A; CDC25A2

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Zap-70(Phospho-Tyr493) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from JK cells untreated or treated with anisomycin using Zap-70(Phospho-Tyr493) Antibody.)

Compare (5 max)

ZAP70, Polyclonal Antibody (Cat# AAA243099)

Full Name

Phospho-ZAP70 (Tyr493) Antibody

Gene Names

ZAP70; SRK; STD; TZK; STCD; ZAP-70

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue using PKCth(Phospho-Ser695) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from 293 cells untreated or treated with PMA using PKCth(Phospho-Ser695) Antibody.)

Compare (5 max)

PRKCQ, Polyclonal Antibody (Cat# AAA243103)

Full Name

Phospho-PRKCQ (Ser695) Antibody

Gene Names

PRKCQ; PRKCT; nPKC-theta

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using SEK1/MKK4(Phospho-Thr261) Antibody.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using SEK1/MKK4(Phospho-Thr261) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

Compare (5 max)

MAP2K4, Polyclonal Antibody (Cat# AAA243105)

Full Name

Phospho-MAP2K4 (Thr261) Antibody

Gene Names

MAP2K4; JNKK; MEK4; MKK4; SEK1; JNKK1; SERK1; MAPKK4; PRKMK4; SAPKK1; SAPKK-1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using MEK1/MEK2(Phospho-Ser217/Ser221) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from Hela cell untreated or treated with UV MEK1/MEK2(Phospho-Ser217/Ser221) Antibody)

Compare (5 max)

MAP2K1, Polyclonal Antibody (Cat# AAA243114)

Full Name

Phospho-MAP2K1 (Ser217/Ser221) Antibody

Gene Names

MAP2K1; MEK1; MKK1; MAPKK1; PRKMK1

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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<< Previous Next >>

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using IkB-e(Phospho-Ser22) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from 293 cells untreated or treated with TNF using IkB-e(Phospho-Ser22) Antibody.)

Compare (5 max)

NFKBIE, Polyclonal Antibody (Cat# AAA243116)

Full Name

Phospho-NFKBIE (Ser22) Antibody

Gene Names

NFKBIE; IKBE

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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<< Previous Next >>

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed MEF cells untreated or treated with IFN using STAT3 (Phospho-Tyr705) Antibody.)

IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human Lung carcinoma tissue using STAT3 (Phospho-Tyr705) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using STAT3 (Phospho-Tyr705) Antibody (left) or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from Hela cells untreated or treated with IFN a using STAT3(Phospho-Tyr705) Antibody.)

Compare (5 max)

STAT3, Polyclonal Antibody (Cat# AAA243353)

Full Name

Phospho-STAT3 (Tyr705) Antibody

Gene Names

STAT3; APRF; HIES

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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<< Previous Next >>

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffinembeddedhuman Lung carcinoma tissue usingAkt (Phospho-Ser473) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from SK-BR-3 cells,treated with insulin and EGF, and pretreated with U0126and LY294002 cells using Akt (Phospho-Ser473) Antibody.)

WB (Western Blot) (Western blot analysis of extracts from 293 cells, treatedwith EGF or calf intestinal phosphatase (CIP), using Akt(Phospho-Ser473) Antibody.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using Akt(Phospho-Ser473) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

Compare (5 max)

AKT1, Polyclonal Antibody (Cat# AAA243355)

Full Name

Phospho-AKT1 (Ser473) Antibody

Gene Names

AKT1; AKT; PKB; RAC; CWS6; PRKBA; PKB-ALPHA; RAC-ALPHA

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of extracts from HepG2 cells, treated with calf intestinal phosphatase (CIP), using HDAC2 (Phospho-Ser394) Antibody.)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells showing nuclear staining using HDAC2(Phospho-Ser394) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using HDAC2(Phospho-Ser394) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from 293 cells untreated or treated with EGF using HDAC2(Phospho-Ser394) Antibody.)

Compare (5 max)

HDAC2, Polyclonal Antibody (Cat# AAA243367)

Full Name

Phospho-HDAC2 (Ser394) Antibody

Gene Names

HDAC2; HD2; RPD3; YAF1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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<< Previous Next >>

WB (Western Blot) (Western blot analysis of extracts from JK cells, treated with G-CSF or calf intestinal phosphatase (CIP), using p56Dok-2 (Phospho-Tyr299) Antibody.)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using p56Dok-2(Phospho-Tyr299) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using p56Dok-2(Phospho-Tyr299) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from Jurkat cells untreated or treated with insulin using p56Dok-2(Phospho-Tyr299) Antibody.)

Compare (5 max)

DOK2, Polyclonal Antibody (Cat# AAA243369)

Full Name

Phospho-DOK2 (Tyr299) Antibody

Gene Names

DOK2; p56DOK; p56dok-2

Reactivity

Human

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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<< Previous Next >>

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin-embedded human lung carcinoma tissue, using eIF2alpha (Phospho-Ser49) Antibody.)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using eIF2a(phospho-Ser49) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using eIF2alpha (Phospho-Ser49) Antibody (left) or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extracts from Hela cells untreated or treated with starvation using eIF2a(phospho-Ser49) Antibody.)

Compare (5 max)

EIF2S1, Polyclonal Antibody (Cat# AAA243374)

Full Name

Phospho-EIF2S1 (Ser49) Antibody

Gene Names

EIF2S1; EIF2; EIF-2; EIF2A; EIF-2A; EIF-2alpha

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin- embedded human lung carcinoma tissue using NFκB-p65 (Phospho-Ser276) antibody.)

IHC (Immunohistochemistry) (Immunohistochemical analysis of paraffin- embedded human breast carcinoma tissue using NFκB-p65 (Phospho-Ser276) antibody.)

IHC (Immunohistochemisry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using NFκB-p65 (Phospho-Ser276) Antibody (left) or the same antibody preincubated with blocking peptide (right).)

WB (Western Blot) (Western blot analysis of extract from Hela cells using NF-κB p65 (phospho-Ser276) antibody. Lane 1: The antibody is not preincubated with blocking peptides. Lane 2: The antibody is preincubated with non- phospho peptide blocking peptides. Lane 3: The antibody is preincubated with phospho peptide blocking peptides.)

IF (Immunofluorescence) (Immunofluorescence staining of methanol-fixed Hela cells using NFκB-p65 (Phospho-Ser276) Antibody.)

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RELA, Polyclonal Antibody (Cat# AAA243384)

Full Name

Phospho-RELA (Ser276) Antibody

Gene Names

RELA; p65; NFKB3

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IF (Immunofluorescence) (Immunofluorescence analysis of NIH/3T3 cells, using 53BP1 (Phospho-Ser25) antibody. The picture on the right is treated with the synthesized peptide.)

IHC (Immunohistochemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using 53BP1 (Phospho-Ser25) antibody. The picture on the right is treated with the synthesized peptide.)

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TP53BP1, Polyclonal Antibody (Cat# AAA243240)

Full Name

Phospho-TP53BP1 (Ser25) Antibody

Gene Names

TP53BP1; p202; 53BP1

Reactivity

Human, Mouse, Rat

Applications

Immunofluorescence, Immunohistochemistry

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from HepG2 cells (Lane 2), using DYN1 (Phospho-Ser778) Antibody. The lane on the left is treated with synthesized peptide.)

WB (Western Blot) (Western blot analysis of extracts from Mouse brain cells, using DYN1 (Phospho-Ser778) antibody. The lane on the right is treated with the synthesized peptide.)

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DNM1, Polyclonal Antibody (Cat# AAA243244)

Full Name

Phospho-DNM1 (Ser778) Antibody

Gene Names

DNM1; DNM

Reactivity

Human, Mouse, Rat

Applications

Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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IHC (Immunohiostchemistry) (Immunohistochemistry analysis of paraffin-embedded human colon carcinoma tissue using WAVE1 (Phospho-Tyr125) antibody. The picture on the right is treated with the synthesized peptide.)

WB (Western Blot) (Western blot analysis of extracts from NIH/3T3 cells, treated with Insulin (0.01U/ml, 15mins), using WAVE1 (Phospho-Tyr125) antibody. The lane on the right is treated with the synthesized peptide.)

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WASF1, Polyclonal Antibody (Cat# AAA243253)

Full Name

Phospho-WASF1 (Tyr125) Antibody

Gene Names

WASF1; WAVE; SCAR1; WAVE1

Reactivity

Human, Mouse

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

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WB (Western Blot) (Western blot analysis of extracts from C6 cells, treated with PMA or calf intestinal phosphatase (CIP), using c-Jun (Phospho-Ser73) Antibody.)

IHC (Immunohiostchemistry) (Immunohistochemical analysis of paraffin-embedded human breast carcinoma tissue using c-Jun(Phospho-Ser73) Antibody(left) or the same antibody preincubated with blocking peptide(right).)

WB (Western Blot) (Western blot analysis of extracts from PC14 cells untreated or treated with EGF using c-Jun(Phospho-Ser73) Antibody.)

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JUN, Polyclonal Antibody (Cat# AAA243256)

Full Name

Phospho-JUN (Ser73) Antibody

Gene Names

JUN; AP1; AP-1; c-Jun

Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Western Blot

Purity

Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatography usin

Pricing

$390/0.1 mL $755/2x0.1 mL $1,105/3x0.1 mL $1,430/4x0.1 mL $1,725/5x0.1 mL

What Are Phospho Antibodies?

Protein phosphorylation is a process where a phosphate group is added to certain amino acid residues of a protein – usually serine (S), threonine (T), or tyrosine (Y) - by enzymes called kinases. This process is integral in controlling cellular signaling, cellular growth, and other biological functions.

Our catalog includes a wide range of phospho-specific antibodies that can accurately detect this important marker. They perform strongly in widely-used laboratory applications such as Western blot, flow cytometry, immunohistochemistry, and immunofluorescence microscopy. We value your trust in us and are committed to providing top-quality products and services. All of our antibodies are guaranteed to work for the applications and species indicated on our website & associated product pages.

What Are The Key Applications of Phospho Antibodies?

1. Western Blotting

One of the first steps a researcher can take in utilizing these phospho-specific antibodies, is to check if the antibody works using a technique referred to as “Western blot”. For those unfamiliar, Western Blot aids in showing whether the protein that the antibody recognizes is appearing at the correct/expected size. These phospho-specific antibodies should also be able to detect changes in the target protein’s phosphorylation (on/off state) when cells are stimulated in certain ways.

2. Staining of Fixed Cells (Immunocytochemistry)

Another routine use of these phospho-specific antibodies, is to test if the antibody is able to demonstrate similar performance when used on fixed cells (intact cells that have been preserved) as it did in the Western blot tests. It is an important aspect in many cases to confirm that the antibody works in actual intact cell samples. Ideally, the method used for cellular fixation should be the same as what is used in pathology labs (like using 10% formalin). To check if the antibody works well in tissue sections (FFPE), researchers will often test it on fixed cells that are processed similar to tissue samples.

3. Specificity Tests Using Peptides

In order to make sure that the antibody is only binding to the right target:

• Laboratory technicians will mix the antibody with phospho-peptides (short segments of the protein containing the phosphate group modification).


• If the antibody signal disappears, it is confirmation that it is binding to the correct phosphorylated location.


• A more robust test is to use both the phosphorylated and non-phosphorylated (dephosphorylated) versions of the protein. The antibody should react only with the phosphorylated one.


• Another method sometimes utilized is to treat the sample with an enzyme, such as alkaline phosphatase, that specifically removes phosphate groups. If the antibody signal disappears after this, it also confirms specificity.

4. Genetic Confirmation

As a final step, scientists can genetically manipulate the nucleotide sequence and alter the target protein by removing the exact site where phosphorylation happens. If the antibody no longer appears to detect the modified protein, it is strong evidence supporting the antibody being specific for that phosphorylated site.

Why Buy Phospho Antibodies Through Us?

• The production laboratory adheres to strict and consistent protocols prior to releasing any of these phospho-specific antibodies:


• Standard methods and proper controls in all tests to ensure high quality.


• These antibodies are tested and validated in different cell types and species.


• High quality control criterion to ensure each batch is consistent, so you will obtain reliable results every time.

FAQ

1. What Are Phospho-Specific Antibodies?

Phospho-specific antibodies are made to detect proteins only when they have a phosphate group linked to a specific amino acid residue. This empowers scientists understand if a protein is "turned on" or active, based on its phosphorylation state.

2. How to Detect Phosphorylated Proteins in a Western Blot?

To find out if a protein is phosphorylated using Western blot:

• Use a phospho-specific antibody that binds only to the phosphorylated form of the protein.


• You can also use a “regular” antibody for the same amino acid sequence of the protein that the phospho-specific antibody is binding to (but in this case, this antibody will not bind if there is a phosphate group present) in order to compare how much of it is phosphorylated versus how much is non-phosphorylated (or “total” protein, if the “normal” antibody’s epitopes are non-phospho-site-specific).

3. How to Choose the Best Antibody?

Here are some simple tips to help you pick the right antibody:

• Know your target


• Match your sample characteristics


• Confirm the intended use is appropriate


• Check “host” and “type”


• Check the “quality” of the presented data/images


• Appraise whether the available validation meets your needs